The Experts below are selected from a list of 306 Experts worldwide ranked by ideXlab platform
Poonam Jethanandani - One of the best experts on this subject based on the ideXlab platform.
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Circulating testosterone levels in Skeletal Fluorosis patients.
Journal of toxicology. Clinical toxicology, 1996Co-Authors: A. K. Susheela, Poonam JethanandaniAbstract:Objective: The present study focusses on serum testosterone concentrations in patients with Skeletal Fluorosis, in order to assess the hormonal status in fluoride toxicity. Methods: Serum testosterones were compared for patients afflicted with Skeletal Fluorosis (n = 30) and healthy males consuming water containing less than 1 ppm fluoride (Control 1, n = 26) and a second category of controls (Control 2, n = 16): individuals living in the same house as the patients and consuming same water as patients but not exhibiting clinical manifestations of Skeletal Fluorosis. Results: Circulating serum testosterones in Skeletal Fluorosis patients were significantly lower than those of Control 1 atp
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circulating testosterone levels in Skeletal Fluorosis patients
Clinical Toxicology, 1996Co-Authors: A. K. Susheela, Poonam JethanandaniAbstract:Objective: The present study focusses on serum testosterone concentrations in patients with Skeletal Fluorosis, in order to assess the hormonal status in fluoride toxicity. Methods: Serum testosterones were compared for patients afflicted with Skeletal Fluorosis (n = 30) and healthy males consuming water containing less than 1 ppm fluoride (Control 1, n = 26) and a second category of controls (Control 2, n = 16): individuals living in the same house as the patients and consuming same water as patients but not exhibiting clinical manifestations of Skeletal Fluorosis. Results: Circulating serum testosterones in Skeletal Fluorosis patients were significantly lower than those of Control 1 atp <0.01. Testosterone concentrations of Control 2 were also lower than those of Control 1 at p <0.05 but were higher than those of the patient group. Conclusion: Decreased testosterone concentrations in Skeletal Fluorosis patients and in males drinking the same water as the patients but with no clinical manifestations of t...
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Serum haptoglobin and C-reactive protein in human Skeletal Fluorosis
Clinical biochemistry, 1994Co-Authors: A. K. Susheela, Poonam JethanandaniAbstract:Circulating levels of haptoglobin and C-reactive protein were studied in patients of Skeletal Fluorosis and compared with two types of controls. The first type of control included normal healthy individuals consuming water containing permissible levels of fluoride (up to 1 mg/L). The second type of control included individuals consuming water contaminated with fluoride (1.2-14.5 mg/L) but not exhibiting clinical manifestations of Skeletal Fluorosis. A significant increase in the levels of haptoglobin (p < 0.01) and C-reactive protein (p < 0.01) as well as a raised erythrocyte sedimentation rate were seen in patients of Skeletal Fluorosis as compared to both types of controls. The present study suggests the possibility of a subclinical inflammatory reaction occurring in patients with Skeletal Fluorosis.
Qun Lou - One of the best experts on this subject based on the ideXlab platform.
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Association between Bone Morphogenetic Protein 2 Gene Polymorphisms and Skeletal Fluorosis of The Brick-tea Type Fluorosis in Tibetans and Kazakhs, China.
International journal of environmental health research, 2021Co-Authors: Qun Lou, Yang Liu, Xiaona Liu, Yanmei Yang, Ning Guo, Wei Huang, Yanhui GaoAbstract:To investigate the potential association between BMP2 single nucleotide polymorphisms (SNPs) and brick-tea-type Skeletal Fluorosis risk in cross-sectional case-control study conducted in Sinkiang and Qinghai, China, a total of 598 individuals, including 308 Tibetans and 290 Kazakhs, were enrolled. Using the standard WS/192-2008 (China), 221 Skeletal Fluorosis cases were diagnosed, including 123 Tibetans and 98 Kazakhs. Logistic regressions 2 analysis did not find the association between SNPs (Rs235764, Rs235739 and Rs996544) and Skeletal Fluorosis. Genetic models, linkage disequilibrium (LD) and haplotype analysis were not found to be associated with risk of Skeletal Fluorosis after adjustment by age and sex (P>0.05).Our data suggested that Rs 235764, Rs 235739 and Rs 996544 were not linked susceptibility for Skeletal Fluorosis in our cross-sectional case-control study.
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FRZB1 rs2242070 polymorphisms is associated with brick tea type Skeletal Fluorosis in Kazakhs, but not in Tibetans, China.
Archives of toxicology, 2018Co-Authors: Yanmei Yang, Yang Liu, Xiaona Liu, Yanru Chu, Yumei Fan, Qiaoshi Zhao, Huazhu Yan, Simeng Huo, Limei Wang, Qun LouAbstract:Skeletal Fluorosis is a metabolic bone and joint disease caused by excessive accumulation of fluoride in the bones. Compared with Kazakhs, Tibetans are more likely to develop moderate and severe brick tea type Skeletal Fluorosis, although they have similar fluoride exposure. Single nucleotide polymorphisms (SNPs) in frizzled-related protein (FRZB) have been associated with osteoarthritis, but their association with the risk of Skeletal Fluorosis has not been reported. In this paper, we investigated the association of three SNPs (rs7775, rs2242070 and rs9288087) in FRZB1with brick tea type Skeletal Fluorosis risk in a cross-sectional case–control study conducted in Sinkiang and Qinghai, China. A total of 598 individuals, including 308 Tibetans and 290 Kazakhs, were enrolled in this study, in which cases and controls were 221 and 377, respectively. The Skeletal Fluorosis was diagnosed according to the Chinese diagnostic criteria of endemic Skeletal Fluorosis (WS192-2008). The fluoride content in tea water or urine was detected using the fluoride ion electrode. SNPs were assessed using the Sequenom MassARRAY system. Binary logistic regressions found evidence of association with rs2242070 AA genotype in only Kazakh participants [odds ratio (OR) 0.417, 95% CI 0.216–0.807, p = 0.009], but not in Tibetans. When stratified by age, this protective effect of AA genotype in rs2242070 was pronounced in Kazakh participants aged 46–65 (OR 0.321, 95% CI 0.135–0.764, p = 0.010). This protective association with AA genotype in rs2242070 in Kazakhs also appeared to be stronger with tea fluoride intake > 3.5 mg/day (OR 0.396, 95% CI 0.182–0.864, p = 0.020). Our data suggest there might be differential genetic influence on Skeletal Fluorosis risk in Kazakh and Tibetan participants and that this difference might be modified by tea fluoride intake.
Yang Liu - One of the best experts on this subject based on the ideXlab platform.
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Association between Bone Morphogenetic Protein 2 Gene Polymorphisms and Skeletal Fluorosis of The Brick-tea Type Fluorosis in Tibetans and Kazakhs, China.
International journal of environmental health research, 2021Co-Authors: Qun Lou, Yang Liu, Xiaona Liu, Yanmei Yang, Ning Guo, Wei Huang, Yanhui GaoAbstract:To investigate the potential association between BMP2 single nucleotide polymorphisms (SNPs) and brick-tea-type Skeletal Fluorosis risk in cross-sectional case-control study conducted in Sinkiang and Qinghai, China, a total of 598 individuals, including 308 Tibetans and 290 Kazakhs, were enrolled. Using the standard WS/192-2008 (China), 221 Skeletal Fluorosis cases were diagnosed, including 123 Tibetans and 98 Kazakhs. Logistic regressions 2 analysis did not find the association between SNPs (Rs235764, Rs235739 and Rs996544) and Skeletal Fluorosis. Genetic models, linkage disequilibrium (LD) and haplotype analysis were not found to be associated with risk of Skeletal Fluorosis after adjustment by age and sex (P>0.05).Our data suggested that Rs 235764, Rs 235739 and Rs 996544 were not linked susceptibility for Skeletal Fluorosis in our cross-sectional case-control study.
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Association between ALOX15 gene polymorphism and brick-tea type Skeletal Fluorosis in Tibetans, Kazaks and Han, China.
International journal of environmental health research, 2019Co-Authors: Yanru Chu, Yang Liu, Limei Wang, Ning Guo, Wei Huang, Lou Qun, Jian Wang, Meichen Zhang, Fanshuo YinAbstract:To evaluate the association between ALOX15 gene polymorphism and Skeletal Fluorosis (SF), a case-control study was conducted. A total of 1023 individuals, including 308 Tibetans, 290 Kazaks and 425...
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FRZB1 rs2242070 polymorphisms is associated with brick tea type Skeletal Fluorosis in Kazakhs, but not in Tibetans, China.
Archives of toxicology, 2018Co-Authors: Yanmei Yang, Yang Liu, Xiaona Liu, Yanru Chu, Yumei Fan, Qiaoshi Zhao, Huazhu Yan, Simeng Huo, Limei Wang, Qun LouAbstract:Skeletal Fluorosis is a metabolic bone and joint disease caused by excessive accumulation of fluoride in the bones. Compared with Kazakhs, Tibetans are more likely to develop moderate and severe brick tea type Skeletal Fluorosis, although they have similar fluoride exposure. Single nucleotide polymorphisms (SNPs) in frizzled-related protein (FRZB) have been associated with osteoarthritis, but their association with the risk of Skeletal Fluorosis has not been reported. In this paper, we investigated the association of three SNPs (rs7775, rs2242070 and rs9288087) in FRZB1with brick tea type Skeletal Fluorosis risk in a cross-sectional case–control study conducted in Sinkiang and Qinghai, China. A total of 598 individuals, including 308 Tibetans and 290 Kazakhs, were enrolled in this study, in which cases and controls were 221 and 377, respectively. The Skeletal Fluorosis was diagnosed according to the Chinese diagnostic criteria of endemic Skeletal Fluorosis (WS192-2008). The fluoride content in tea water or urine was detected using the fluoride ion electrode. SNPs were assessed using the Sequenom MassARRAY system. Binary logistic regressions found evidence of association with rs2242070 AA genotype in only Kazakh participants [odds ratio (OR) 0.417, 95% CI 0.216–0.807, p = 0.009], but not in Tibetans. When stratified by age, this protective effect of AA genotype in rs2242070 was pronounced in Kazakh participants aged 46–65 (OR 0.321, 95% CI 0.135–0.764, p = 0.010). This protective association with AA genotype in rs2242070 in Kazakhs also appeared to be stronger with tea fluoride intake > 3.5 mg/day (OR 0.396, 95% CI 0.182–0.864, p = 0.020). Our data suggest there might be differential genetic influence on Skeletal Fluorosis risk in Kazakh and Tibetan participants and that this difference might be modified by tea fluoride intake.
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Matrix Metallopeptidase-2 Gene rs2287074 Polymorphism is Associated with Brick Tea Skeletal Fluorosis in Tibetans and Kazaks, China.
Scientific reports, 2017Co-Authors: Junrui Pei, Yang Liu, Xiaona Liu, Yanru Chu, Qing Yang, Wei Jiang, Fuxun Chen, Gottfried M. DarkoAbstract:Brick tea Skeletal Fluorosis is still a public health issue in the north-western area of China. However its pathogenesis remains unknown. Our previous study reveals that the severity of Skeletal Fluorosis in Tibetans is more serious than that in Kazaks, although they have similar fluoride exposure, suggesting the onset of brick tea type Skeletal Fluorosis might be genetically influenced. Here we show that MMP-2 rs2287074 SNP (G/A), but not rs243865, was associated with Brick tea type Fluorosis in Tibetans and Kazaks, China. The trend test reveals a decline in probability for Skeletal Fluorosis with increasing number of A alleles in Tibetans. After controlling potential confounders, AA genotype had about 80 percent lower probability of developing Skeletal Fluorosis than GG genotype in Tibetans (odds ratio = 0.174, 95% CI: 0.053, 0.575), and approximately 53 percent lower probability in Kazaks (odds ratio = 0.462, 95% CI: 0.214, 0.996). A meta-analysis shows that the AA genotype had approximately 63 percent lower odds (odds ratio = 0.373, 95% CI: 0.202, 0.689) compared with GG genotype within the two ethnicities. A significant correlation was also found between the genotype of MMP2 rs2287074 and Skeletal Fluorosis severity. Therefore, the A allele of MMP2 rs2287074 could be a protective factor for brick tea Skeletal Fluorosis.
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Relationship between Rs1801726 polymorphism of calcium sensing receptor gene and Skeletal Fluorosis
2016Co-Authors: Yang Liu, Liting Song, Yanru Chu, Qing Yang, Wei Jiang, Ying Bao, Fuxun ChenAbstract:Objective To investigate the relationship between single nucleotide polymorphism of the calcium sensing receptor gene (CaSR) Rs1801726 and Skeletal Fluorosis. Methods From 2012 to 2013, the Tibetan living area in Guoluo of Qinghai Province, the Mongolian, Russian living area in Hulun Buir City of the Inner Mongolia Autonomous Region and the Kazakh living area in Altay of Xinjiang Uygur Autonomous Region, two counties were chosen in each area and 3 to 4 drinking brick-tea type Fluorosis districts were chosen as the investigated districts in the counties. Totally 347 patients with Skeletal Fluorosis diagnosed by X-ray were selected as Skeletal Fluorosis group, 1 067 people without Skeletal Fluorosis as non Skeletal Fluorosis group. Tibean, Kazak, Mongolia, Han, Russian were 308, 290, 261, 425, 130 people, respectively. A questionnaire survey of endemic Fluorosis was conducted, the survey contents included general characteristics, average daily brick tea intake; 5 ml venous blood was collected, the single nucleotide polymorphism of Rs1801726 was detected by mass spectrometry; drinking water, brick tea and urine samples were collected and analyzed; forearm, lumbar spine and pelvis of subjects were examined by Portable DR. Water fluoride, brick tea fluorine and urinary fluoride were detected by ion selective electrode (WS/T 89-2006). X-ray diagnosis of Skeletal Fluorosis was based on "Endemic Skeletal Fluorosis Diagnostic Criteria" (WS/T 192-2007), and epidemiological and clinical descriptive information were combined. Results By the Hardy-Weinberg balance test, the CaSR Rs1801726 gene loci genotype was representative in the group of Skeletal Fluorosis, the group of non Skeletal Fluorosis and each nationality (all P > 0.05). The difference of CaSR gene Rs1801726 genotype (CC, CG) in the group of Skeletal Fluorosis and the group of non Skeletal Fluorosis was not statistically significant [crude OR value was 0.919 (0.509-1.658) and the adjusted OR value was 0.979 (0.521-1.840)] with binary Logistic regression analysis. The difference of CaSR gene Rs1801726 genotype (CC, CG) in the group of Skeletal Fluorosis and the group of non Skeletal Fluorosis in different nationality was not statistically significant [Tibetan: crude OR value was 1.512 (0.210-10.882) and the adjusted OR value was 1.700 (0.224-12.908); Kazak: crude OR value was 1.517 (0.616-3.734) and the adjusted OR value was 1.444 (0.568-3.671); Mongolia: crude OR value was 1.000 (0.202-4.949) and the adjusted OR value was 1.036 (0.189-5.671); Han: crude OR value was 0.369 (0.048-2.825) and the adjusted OR value was 0.301 (0.036-2.551); Russian: crude OR value was 0.892 (0.105-7.582) and the adjusted OR value was 0.555 (0.060-5.098)] with binary Logistic regression analysis. Conclusion In this study, the polymorphism of CaSR Rs1801726 single nucleotide polymorphism is not related to the prevalence of Fluorosis in different ethnic groups in our country. Key words: Receptors, calcium-sensing; Polymorphism, single nucleotide; Fluorosis, dental
Yanru Chu - One of the best experts on this subject based on the ideXlab platform.
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Association between ALOX15 gene polymorphism and brick-tea type Skeletal Fluorosis in Tibetans, Kazaks and Han, China.
International journal of environmental health research, 2019Co-Authors: Yanru Chu, Yang Liu, Limei Wang, Ning Guo, Wei Huang, Lou Qun, Jian Wang, Meichen Zhang, Fanshuo YinAbstract:To evaluate the association between ALOX15 gene polymorphism and Skeletal Fluorosis (SF), a case-control study was conducted. A total of 1023 individuals, including 308 Tibetans, 290 Kazaks and 425...
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FRZB1 rs2242070 polymorphisms is associated with brick tea type Skeletal Fluorosis in Kazakhs, but not in Tibetans, China.
Archives of toxicology, 2018Co-Authors: Yanmei Yang, Yang Liu, Xiaona Liu, Yanru Chu, Yumei Fan, Qiaoshi Zhao, Huazhu Yan, Simeng Huo, Limei Wang, Qun LouAbstract:Skeletal Fluorosis is a metabolic bone and joint disease caused by excessive accumulation of fluoride in the bones. Compared with Kazakhs, Tibetans are more likely to develop moderate and severe brick tea type Skeletal Fluorosis, although they have similar fluoride exposure. Single nucleotide polymorphisms (SNPs) in frizzled-related protein (FRZB) have been associated with osteoarthritis, but their association with the risk of Skeletal Fluorosis has not been reported. In this paper, we investigated the association of three SNPs (rs7775, rs2242070 and rs9288087) in FRZB1with brick tea type Skeletal Fluorosis risk in a cross-sectional case–control study conducted in Sinkiang and Qinghai, China. A total of 598 individuals, including 308 Tibetans and 290 Kazakhs, were enrolled in this study, in which cases and controls were 221 and 377, respectively. The Skeletal Fluorosis was diagnosed according to the Chinese diagnostic criteria of endemic Skeletal Fluorosis (WS192-2008). The fluoride content in tea water or urine was detected using the fluoride ion electrode. SNPs were assessed using the Sequenom MassARRAY system. Binary logistic regressions found evidence of association with rs2242070 AA genotype in only Kazakh participants [odds ratio (OR) 0.417, 95% CI 0.216–0.807, p = 0.009], but not in Tibetans. When stratified by age, this protective effect of AA genotype in rs2242070 was pronounced in Kazakh participants aged 46–65 (OR 0.321, 95% CI 0.135–0.764, p = 0.010). This protective association with AA genotype in rs2242070 in Kazakhs also appeared to be stronger with tea fluoride intake > 3.5 mg/day (OR 0.396, 95% CI 0.182–0.864, p = 0.020). Our data suggest there might be differential genetic influence on Skeletal Fluorosis risk in Kazakh and Tibetan participants and that this difference might be modified by tea fluoride intake.
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Matrix Metallopeptidase-2 Gene rs2287074 Polymorphism is Associated with Brick Tea Skeletal Fluorosis in Tibetans and Kazaks, China.
Scientific reports, 2017Co-Authors: Junrui Pei, Yang Liu, Xiaona Liu, Yanru Chu, Qing Yang, Wei Jiang, Fuxun Chen, Gottfried M. DarkoAbstract:Brick tea Skeletal Fluorosis is still a public health issue in the north-western area of China. However its pathogenesis remains unknown. Our previous study reveals that the severity of Skeletal Fluorosis in Tibetans is more serious than that in Kazaks, although they have similar fluoride exposure, suggesting the onset of brick tea type Skeletal Fluorosis might be genetically influenced. Here we show that MMP-2 rs2287074 SNP (G/A), but not rs243865, was associated with Brick tea type Fluorosis in Tibetans and Kazaks, China. The trend test reveals a decline in probability for Skeletal Fluorosis with increasing number of A alleles in Tibetans. After controlling potential confounders, AA genotype had about 80 percent lower probability of developing Skeletal Fluorosis than GG genotype in Tibetans (odds ratio = 0.174, 95% CI: 0.053, 0.575), and approximately 53 percent lower probability in Kazaks (odds ratio = 0.462, 95% CI: 0.214, 0.996). A meta-analysis shows that the AA genotype had approximately 63 percent lower odds (odds ratio = 0.373, 95% CI: 0.202, 0.689) compared with GG genotype within the two ethnicities. A significant correlation was also found between the genotype of MMP2 rs2287074 and Skeletal Fluorosis severity. Therefore, the A allele of MMP2 rs2287074 could be a protective factor for brick tea Skeletal Fluorosis.
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Relationship between Rs1801726 polymorphism of calcium sensing receptor gene and Skeletal Fluorosis
2016Co-Authors: Yang Liu, Liting Song, Yanru Chu, Qing Yang, Wei Jiang, Ying Bao, Fuxun ChenAbstract:Objective To investigate the relationship between single nucleotide polymorphism of the calcium sensing receptor gene (CaSR) Rs1801726 and Skeletal Fluorosis. Methods From 2012 to 2013, the Tibetan living area in Guoluo of Qinghai Province, the Mongolian, Russian living area in Hulun Buir City of the Inner Mongolia Autonomous Region and the Kazakh living area in Altay of Xinjiang Uygur Autonomous Region, two counties were chosen in each area and 3 to 4 drinking brick-tea type Fluorosis districts were chosen as the investigated districts in the counties. Totally 347 patients with Skeletal Fluorosis diagnosed by X-ray were selected as Skeletal Fluorosis group, 1 067 people without Skeletal Fluorosis as non Skeletal Fluorosis group. Tibean, Kazak, Mongolia, Han, Russian were 308, 290, 261, 425, 130 people, respectively. A questionnaire survey of endemic Fluorosis was conducted, the survey contents included general characteristics, average daily brick tea intake; 5 ml venous blood was collected, the single nucleotide polymorphism of Rs1801726 was detected by mass spectrometry; drinking water, brick tea and urine samples were collected and analyzed; forearm, lumbar spine and pelvis of subjects were examined by Portable DR. Water fluoride, brick tea fluorine and urinary fluoride were detected by ion selective electrode (WS/T 89-2006). X-ray diagnosis of Skeletal Fluorosis was based on "Endemic Skeletal Fluorosis Diagnostic Criteria" (WS/T 192-2007), and epidemiological and clinical descriptive information were combined. Results By the Hardy-Weinberg balance test, the CaSR Rs1801726 gene loci genotype was representative in the group of Skeletal Fluorosis, the group of non Skeletal Fluorosis and each nationality (all P > 0.05). The difference of CaSR gene Rs1801726 genotype (CC, CG) in the group of Skeletal Fluorosis and the group of non Skeletal Fluorosis was not statistically significant [crude OR value was 0.919 (0.509-1.658) and the adjusted OR value was 0.979 (0.521-1.840)] with binary Logistic regression analysis. The difference of CaSR gene Rs1801726 genotype (CC, CG) in the group of Skeletal Fluorosis and the group of non Skeletal Fluorosis in different nationality was not statistically significant [Tibetan: crude OR value was 1.512 (0.210-10.882) and the adjusted OR value was 1.700 (0.224-12.908); Kazak: crude OR value was 1.517 (0.616-3.734) and the adjusted OR value was 1.444 (0.568-3.671); Mongolia: crude OR value was 1.000 (0.202-4.949) and the adjusted OR value was 1.036 (0.189-5.671); Han: crude OR value was 0.369 (0.048-2.825) and the adjusted OR value was 0.301 (0.036-2.551); Russian: crude OR value was 0.892 (0.105-7.582) and the adjusted OR value was 0.555 (0.060-5.098)] with binary Logistic regression analysis. Conclusion In this study, the polymorphism of CaSR Rs1801726 single nucleotide polymorphism is not related to the prevalence of Fluorosis in different ethnic groups in our country. Key words: Receptors, calcium-sensing; Polymorphism, single nucleotide; Fluorosis, dental
Xiaona Liu - One of the best experts on this subject based on the ideXlab platform.
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Association between Bone Morphogenetic Protein 2 Gene Polymorphisms and Skeletal Fluorosis of The Brick-tea Type Fluorosis in Tibetans and Kazakhs, China.
International journal of environmental health research, 2021Co-Authors: Qun Lou, Yang Liu, Xiaona Liu, Yanmei Yang, Ning Guo, Wei Huang, Yanhui GaoAbstract:To investigate the potential association between BMP2 single nucleotide polymorphisms (SNPs) and brick-tea-type Skeletal Fluorosis risk in cross-sectional case-control study conducted in Sinkiang and Qinghai, China, a total of 598 individuals, including 308 Tibetans and 290 Kazakhs, were enrolled. Using the standard WS/192-2008 (China), 221 Skeletal Fluorosis cases were diagnosed, including 123 Tibetans and 98 Kazakhs. Logistic regressions 2 analysis did not find the association between SNPs (Rs235764, Rs235739 and Rs996544) and Skeletal Fluorosis. Genetic models, linkage disequilibrium (LD) and haplotype analysis were not found to be associated with risk of Skeletal Fluorosis after adjustment by age and sex (P>0.05).Our data suggested that Rs 235764, Rs 235739 and Rs 996544 were not linked susceptibility for Skeletal Fluorosis in our cross-sectional case-control study.
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FRZB1 rs2242070 polymorphisms is associated with brick tea type Skeletal Fluorosis in Kazakhs, but not in Tibetans, China.
Archives of toxicology, 2018Co-Authors: Yanmei Yang, Yang Liu, Xiaona Liu, Yanru Chu, Yumei Fan, Qiaoshi Zhao, Huazhu Yan, Simeng Huo, Limei Wang, Qun LouAbstract:Skeletal Fluorosis is a metabolic bone and joint disease caused by excessive accumulation of fluoride in the bones. Compared with Kazakhs, Tibetans are more likely to develop moderate and severe brick tea type Skeletal Fluorosis, although they have similar fluoride exposure. Single nucleotide polymorphisms (SNPs) in frizzled-related protein (FRZB) have been associated with osteoarthritis, but their association with the risk of Skeletal Fluorosis has not been reported. In this paper, we investigated the association of three SNPs (rs7775, rs2242070 and rs9288087) in FRZB1with brick tea type Skeletal Fluorosis risk in a cross-sectional case–control study conducted in Sinkiang and Qinghai, China. A total of 598 individuals, including 308 Tibetans and 290 Kazakhs, were enrolled in this study, in which cases and controls were 221 and 377, respectively. The Skeletal Fluorosis was diagnosed according to the Chinese diagnostic criteria of endemic Skeletal Fluorosis (WS192-2008). The fluoride content in tea water or urine was detected using the fluoride ion electrode. SNPs were assessed using the Sequenom MassARRAY system. Binary logistic regressions found evidence of association with rs2242070 AA genotype in only Kazakh participants [odds ratio (OR) 0.417, 95% CI 0.216–0.807, p = 0.009], but not in Tibetans. When stratified by age, this protective effect of AA genotype in rs2242070 was pronounced in Kazakh participants aged 46–65 (OR 0.321, 95% CI 0.135–0.764, p = 0.010). This protective association with AA genotype in rs2242070 in Kazakhs also appeared to be stronger with tea fluoride intake > 3.5 mg/day (OR 0.396, 95% CI 0.182–0.864, p = 0.020). Our data suggest there might be differential genetic influence on Skeletal Fluorosis risk in Kazakh and Tibetan participants and that this difference might be modified by tea fluoride intake.
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Matrix Metallopeptidase-2 Gene rs2287074 Polymorphism is Associated with Brick Tea Skeletal Fluorosis in Tibetans and Kazaks, China.
Scientific reports, 2017Co-Authors: Junrui Pei, Yang Liu, Xiaona Liu, Yanru Chu, Qing Yang, Wei Jiang, Fuxun Chen, Gottfried M. DarkoAbstract:Brick tea Skeletal Fluorosis is still a public health issue in the north-western area of China. However its pathogenesis remains unknown. Our previous study reveals that the severity of Skeletal Fluorosis in Tibetans is more serious than that in Kazaks, although they have similar fluoride exposure, suggesting the onset of brick tea type Skeletal Fluorosis might be genetically influenced. Here we show that MMP-2 rs2287074 SNP (G/A), but not rs243865, was associated with Brick tea type Fluorosis in Tibetans and Kazaks, China. The trend test reveals a decline in probability for Skeletal Fluorosis with increasing number of A alleles in Tibetans. After controlling potential confounders, AA genotype had about 80 percent lower probability of developing Skeletal Fluorosis than GG genotype in Tibetans (odds ratio = 0.174, 95% CI: 0.053, 0.575), and approximately 53 percent lower probability in Kazaks (odds ratio = 0.462, 95% CI: 0.214, 0.996). A meta-analysis shows that the AA genotype had approximately 63 percent lower odds (odds ratio = 0.373, 95% CI: 0.202, 0.689) compared with GG genotype within the two ethnicities. A significant correlation was also found between the genotype of MMP2 rs2287074 and Skeletal Fluorosis severity. Therefore, the A allele of MMP2 rs2287074 could be a protective factor for brick tea Skeletal Fluorosis.
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Role of global histone H3 acetylation in patients with Skeletal Fluorosis
2016Co-Authors: Zhipeng Fan, Jing Sun, Yang Liu, Xiaona Liu, Dianjun Sun, Yanhui GaoAbstract:Objective To explore the relationships between different levels of fluoride, the global histone H3 acetylation in peripheral blood mononuclear cells (PBMC) and the condition of Skeletal Fluorosis, which might be helpful in explaining the mechanism of Skeletal Fluorosis. Methods In 2012, two counties were chosen in each area, the Tibetan living area in Guoluo of Qinghai Province and the Kazakh living area in Altay of Xinjiang Uygur Autonomous Region and 3 to 4 drinking brick-tea type Fluorosis districts were chosen as the investigated districts in the counties. Totally 59 patients with Skeletal Fluorosis in the typical brick-tea type Fluorosis districts were selected, and 59 healthy controls were selected based on age (difference ≤3 years old) and nationality. The adults were investigated by questionnaire, brick-tea water (or buttered tea), urine and 2 ml peripheral blood samples were collected, and their Skeletal Fluorosis was diagnosed according to the national criteria. The fluoride concentrations in brick-tea water (or Buttered tea) and urine were detected by fluoride ion selective electrode. The global histone H3 acetylation was quantified using colorimetric methord. Results The global histone H3 acetylation in PBMC between female and male population (female: 20.05 mg/g and male: 19.94 mg/g) was not significantly different (U = 1 664.5,P > 0.05). The global histone H3 acetylation in PBMC between Tibetans (17.73 mg/g) and Kazakans (20.49 mg/g) was not significantly different (U =-0.902,P > 0.05). However, global histone H3 acetylation and age was positively correlated (r =-0.213,P 3.25-8.05, > 8.05-12.33, > 12.33 mg) and global histone H3 acetylation in PBMC (21.12, 20.05, 19.94 and 35.04 mg/g,H = 1.706,P > 0.05). There was also no significant difference between urine fluoride (4 group: 0-1.69,> 1.69-2.64, > 2.64-4.00, > 4.00 mg/L) and global histone H3 acetylation in PBMC (24.42, 18.13, 19.80 and 28.90 mg/g,H = 5.928,P > 0.05). Global histone H3 acetylation in PBMC from Fluorosis patients (21.69 mg/g) and control healthy subjects was similar (19.43 mg/g,Z =-1.39,P > 0.05). The difference among the different conditions of Skeletal Fluorosis (normal group, mild group and moderate group) was not significant (median: 19.43, 21.32 and 35.95 mg/g,H = 1.692,P > 0.05). Conclusions High fluoride exposure maybe not the factors influencing the global histone H3 acetylation in PBMC. Key words: Fluorosis, skeleton; Histone; Acetylation
