The Experts below are selected from a list of 10155 Experts worldwide ranked by ideXlab platform
Stefano Mantero - One of the best experts on this subject based on the ideXlab platform.
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Electrospun degradable polyesterurethane membranes: potential scaffolds for Skeletal Muscle Tissue engineering.
Biomaterials, 2005Co-Authors: Stefano ManteroAbstract:Skeletal Muscle Tissue engineering represents an attractive approach to overcome problems associated with autologous transfer of Muscle Tissue and provides a valid alternative in Muscle regeneration enhancement. The aim of this study was to investigate the suitability, as scaffold for Skeletal Muscle Tissue engineering, of a known biodegradable block copolymer (DegraPol) processed by electrospinning in the novel form of microfibrous membranes. Scaffolds were characterized with reference to their morphological, degradative and mechanical properties. Subsequently, cell viability, adhesion and differentiation on coated and uncoated DegraPol) slides were investigated using line cells (C2C12 and L6) and primary human satellite cells (HSCs). The membranes exhibited absence of toxic residuals and satisfactory mechanical properties (linear elastic behavior up to 10% deformation, E modulus in the order of magnitude of MPa). A promising cellular response was also found in preliminary experiments: both line cells and HSCs adhered, proliferated and fused on differently coated electrospun membranes. Positive staining for myosin heavy chain expression indicated that differentiation of C2C12 multinucleated cells occurred within the porous elastomeric substrate. Together the results of this study provide significant evidence of the suitability of electrospun DegraPol) membranes as scaffolds for Skeletal Muscle Tissue engineering and that they represent a promising alternative to scaffolds currently used in this field.
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Electrospun degradable polyesterurethane membranes: Potential scaffolds for Skeletal Muscle Tissue engineering
Biomaterials, 2005Co-Authors: S. A. Riboldi, Maurilio Sampaolesi, Peter Neuenschwander, Giulio Cossu, Stefano ManteroAbstract:Skeletal Muscle Tissue engineering represents an attractive approach to overcome problems associated with autologous transfer of Muscle Tissue and provides a valid alternative in Muscle regeneration enhancement. The aim of this study was to investigate the suitability, as scaffold for Skeletal Muscle Tissue engineering, of a known biodegradable block copolymer (DegraPol®) processed by electrospinning in the novel form of microfibrous membranes. Scaffolds were characterized with reference to their morphological, degradative and mechanical properties. Subsequently, cell viability, adhesion and differentiation on coated and uncoated DegraPol®slides were investigated using line cells (C2C12 and L6) and primary human satellite cells (HSCs). The membranes exhibited absence of toxic residuals and satisfactory mechanical properties (linear elastic behavior up to 10% deformation, E modulus in the order of magnitude of MPa). A promising cellular response was also found in preliminary experiments: both line cells and HSCs adhered, proliferated and fused on differently coated electrospun membranes. Positive staining for myosin heavy chain expression indicated that differentiation of C2C12 multinucleated cells occurred within the porous elastomeric substrate. Together the results of this study provide significant evidence of the suitability of electrospun DegraPol®membranes as scaffolds for Skeletal Muscle Tissue engineering and that they represent a promising alternative to scaffolds currently used in this field. © 2004 Elsevier Ltd. All rights reserved.
Adam W. Feinberg - One of the best experts on this subject based on the ideXlab platform.
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Engineering Aligned Skeletal Muscle Tissue Using Decellularized Plant-Derived Scaffolds
ACS Biomaterials Science & Engineering, 2020Co-Authors: Ya-wen Cheng, Daniel J. Shiwarski, Rebecca L. Ball, Kathryn A. Whitehead, Adam W. FeinbergAbstract:In order to achieve organization and function, engineered Tissues require a scaffold that supports cell adhesion, alignment, growth, and differentiation. For Skeletal Muscle Tissue engineering, dec...
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engineered Skeletal Muscle Tissue for soft robotics fabrication strategies current applications and future challenges
Wiley Interdisciplinary Reviews-nanomedicine and Nanobiotechnology, 2014Co-Authors: R M Duffy, Adam W. FeinbergAbstract:Skeletal Muscle is a scalable actuator system used throughout nature from the millimeter to meter length scales and over a wide range of frequencies and force regimes. This adaptability has spurred interest in using engineered Skeletal Muscle to power soft robotics devices and in biotechnology and medical applications. However, the challenges to doing this are similar to those facing the Tissue engineering and regenerative medicine fields; specifically, how do we translate our understanding of myogenesis in vivo to the engineering of Muscle constructs in vitro to achieve functional integration with devices. To do this researchers are developing a number of ways to engineer the cellular microenvironment to guide Skeletal Muscle Tissue formation. This includes understanding the role of substrate stiffness and the mechanical environment, engineering the spatial organization of biochemical and physical cues to guide Muscle alignment, and developing bioreactors for mechanical and electrical conditioning. Examples of engineered Skeletal Muscle that can potentially be used in soft robotics include 2D cantilever-based Skeletal Muscle actuators and 3D Skeletal Muscle Tissues engineered using scaffolds or directed self-organization. Integration into devices has led to basic Muscle-powered devices such as grippers and pumps as well as more sophisticated Muscle-powered soft robots that walk and swim. Looking forward, current, and future challenges include identifying the best source of Muscle precursor cells to expand and differentiate into myotubes, replacing cardiomyocytes with Skeletal Muscle Tissue as the bio-actuator of choice for soft robots, and vascularization and innervation to enable control and nourishment of larger Muscle Tissue constructs. For further resources related to this article, please visit the WIREs website. Conflict of interest: The authors have declared no conflicts of interest for this article.
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Engineered Skeletal Muscle Tissue for soft robotics: fabrication strategies, current applications, and future challenges
Wiley Interdiscip Rev Nanomed Nanobiotechnol, 2014Co-Authors: R M Duffy, Adam W. FeinbergAbstract:Skeletal Muscle is a scalable actuator system used throughout nature from the millimeter to meter length scales and over a wide range of frequencies and force regimes. This adaptability has spurred interest in using engineered Skeletal Muscle to power soft robotics devices and in biotechnology and medical applications. However, the challenges to doing this are similar to those facing the Tissue engineering and regenerative medicine fields; specifically, how do we translate our understanding of myogenesis in vivo to the engineering of Muscle constructs in vitro to achieve functional integration with devices. To do this researchers are developing a number of ways to engineer the cellular microenvironment to guide Skeletal Muscle Tissue formation. This includes understanding the role of substrate stiffness and the mechanical environment, engineering the spatial organization of biochemical and physical cues to guide Muscle alignment, and developing bioreactors for mechanical and electrical conditioning. Examples of engineered Skeletal Muscle that can potentially be used in soft robotics include 2D cantilever-based Skeletal Muscle actuators and 3D Skeletal Muscle Tissues engineered using scaffolds or directed self-organization. Integration into devices has led to basic Muscle-powered devices such as grippers and pumps as well as more sophisticated Muscle-powered soft robots that walk and swim. Looking forward, current, and future challenges include identifying the best source of Muscle precursor cells to expand and differentiate into myotubes, replacing cardiomyocytes with Skeletal Muscle Tissue as the bio-actuator of choice for soft robots, and vascularization and innervation to enable control and nourishment of larger Muscle Tissue constructs.
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Engineered Skeletal Muscle Tissue for soft robotics: fabrication strategies, current applications, and future challenges. - PubMed - NCBI
Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology, 2013Co-Authors: R M Duffy, Adam W. FeinbergAbstract:Skeletal Muscle is a scalable actuator system used throughout nature from the millimeter to meter length scales and over a wide range of frequencies and force regimes. This adaptability has spurred interest in using engineered Skeletal Muscle to power soft robotics devices and in biotechnology and medical applications. However, the challenges to doing this are similar to those facing the Tissue engineering and regenerative medicine fields; specifically, how do we translate our understanding of myogenesis in vivo to the engineering of Muscle constructs in vitro to achieve functional integration with devices. To do this researchers are developing a number of ways to engineer the cellular microenvironment to guide Skeletal Muscle Tissue formation. This includes understanding the role of substrate stiffness and the mechanical environment, engineering the spatial organization of biochemical and physical cues to guide Muscle alignment, and developing bioreactors for mechanical and electrical conditioning. Examples of engineered Skeletal Muscle that can potentially be used in soft robotics include 2D cantilever-based Skeletal Muscle actuators and 3D Skeletal Muscle Tissues engineered using scaffolds or directed self-organization. Integration into devices has led to basic Muscle-powered devices such as grippers and pumps as well as more sophisticated Muscle-powered soft robots that walk and swim. Looking forward, current, and future challenges include identifying the best source of Muscle precursor cells to expand and differentiate into myotubes, replacing cardiomyocytes with Skeletal Muscle Tissue as the bio-actuator of choice for soft robots, and vascularization and innervation to enable control and nourishment of larger Muscle Tissue constructs.
Mengjiy Wang - One of the best experts on this subject based on the ideXlab platform.
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grooved plga films incorporated with rgd yigsr peptides for potential application on Skeletal Muscle Tissue engineering
Colloids and Surfaces B: Biointerfaces, 2013Co-Authors: Peng Yuan Wang, Tsung Han Wu, Weibor Tsai, Mengjiy WangAbstract:Abstract Alignment of myocytes or myotubes is critical for Skeletal Muscle Tissue engineering. In this study, grooved PLGA films (800 nm in width of ridge/groove and 600 nm in depth) incorporated with RGD or YIGSR peptides were fabricated to evaluate its efficacy for Skeletal Muscle Tissue engineering. The growth and differentiation of C2C12 myoblasts were enhanced by the presentation of RGD or YIGSR compared with the untreated PLGA control. On the other hand, cell morphology was guided by the grooved structure, i.e. alignment of myoblasts and myotubes with the direction of grooves. This study elucidates the effects of both surface biochemical and topographic cues on the proliferation and differentiation of C2C12 myoblasts on biodegradable polymer films. Combination of surface topography and peptide presentation has a great potential in designing scaffolds for Skeletal Muscle Tissue engineering.
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Grooved PLGA films incorporated with RGD/YIGSR peptides for potential application on Skeletal Muscle Tissue engineering.
Colloids and Surfaces B: Biointerfaces, 2013Co-Authors: Peng Yuan Wang, Tsung Han Wu, Weibor Tsai, Mengjiy WangAbstract:Abstract Alignment of myocytes or myotubes is critical for Skeletal Muscle Tissue engineering. In this study, grooved PLGA films (800 nm in width of ridge/groove and 600 nm in depth) incorporated with RGD or YIGSR peptides were fabricated to evaluate its efficacy for Skeletal Muscle Tissue engineering. The growth and differentiation of C2C12 myoblasts were enhanced by the presentation of RGD or YIGSR compared with the untreated PLGA control. On the other hand, cell morphology was guided by the grooved structure, i.e. alignment of myoblasts and myotubes with the direction of grooves. This study elucidates the effects of both surface biochemical and topographic cues on the proliferation and differentiation of C2C12 myoblasts on biodegradable polymer films. Combination of surface topography and peptide presentation has a great potential in designing scaffolds for Skeletal Muscle Tissue engineering.
Masamichi Kamihira - One of the best experts on this subject based on the ideXlab platform.
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Functional evaluation of artificial Skeletal Muscle Tissue constructs fabricated by a magnetic force-based Tissue engineering technique
Tissue Engineering Part A, 2020Co-Authors: Yasunori Yamamoto, Hideaki Fujita, Eiji Nagamori, Yoshinori Kawabe, Masamichi KamihiraAbstract:Skeletal Muscle Tissue engineering is currently applied in a variety of research fields, including regenerative medicine, drug screening, and bioactuator development, all of which require the fabrication of biomimic and functional Skeletal Muscle Tissues. In the present study, magnetite cationic liposomes were used to magnetically label C2C12 myoblast cells for the construction of three-dimensional artificial Skeletal Muscle Tissues by an applied magnetic force. Skeletal Muscle functions, such as biochemical and contractile properties, were evaluated for the artificial Tissue constructs. Histological studies revealed that elongated and multinucleated myotubes were observed within the Tissue. Expression of Muscle-specific markers, such as myogenin, myosin heavy chain and tropomyosin, were detected in the Tissue constructs by western blot analysis. Further, creatine kinase activity increased during differentiation. In response to electric pulses, the artificial Tissue constructs contracted to generate a phy...
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Enhancement of contractile force generation of artificial Skeletal Muscle Tissues by mild and transient heat treatment.
Current Pharmaceutical Biotechnology, 2020Co-Authors: Masanori Sato, Yoshinori Kawabe, Kazushi Ikeda, Shota Kanno, Masamichi KamihiraAbstract:Artificial Skeletal Muscle Tissues composed of cells are expected to be used for applications of regenerative medicine and drug screening. Generally, however, the physical forces generated by Tissue-engineered Skeletal Muscle are lower than those of Skeletal Muscle Tissues found in the body. Local hyperthermia is used for many diseases including Muscle injuries. It was recently reported that mild heat treatment improved Skeletal Muscle functions. In this study, we investigated the effects of mild heat treatment on the Tissue-engineered Skeletal Muscle Tissues in vitro. We used magnetite cationic liposomes to label C2C12 myoblast cells magnetically, and constructed densely packed artificial Skeletal Muscle Tissues by using magnetic force. Cell culture at 39°C promoted the differentiation of myoblast cells into myotubes. Moreover, the mild and transient heat treatment improved the contractile properties of artificial Skeletal Muscle Tissue constructs. These findings indicate that the culture method using heat treatment is a useful approach to enhance functions of artificial Skeletal Muscle Tissue.
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Functional evaluation of artificial Skeletal Muscle Tissue constructs fabricated by a magnetic force-based Tissue engineering technique.
Tissue engineering. Part A, 2010Co-Authors: Yasunori Yamamoto, Hideaki Fujita, Eiji Nagamori, Yoshinori Kawabe, Masamichi KamihiraAbstract:Skeletal Muscle Tissue engineering is currently applied in a variety of research fields, including regenerative medicine, drug screening, and bioactuator development, all of which require the fabrication of biomimic and functional Skeletal Muscle Tissues. In the present study, magnetite cationic liposomes were used to magnetically label C2C12 myoblast cells for the construction of three-dimensional artificial Skeletal Muscle Tissues by an applied magnetic force. Skeletal Muscle functions, such as biochemical and contractile properties, were evaluated for the artificial Tissue constructs. Histological studies revealed that elongated and multinucleated myotubes were observed within the Tissue. Expression of Muscle-specific markers, such as myogenin, myosin heavy chain and tropomyosin, were detected in the Tissue constructs by western blot analysis. Further, creatine kinase activity increased during differentiation. In response to electric pulses, the artificial Tissue constructs contracted to generate a physical force (the maximum twitch force, 33.2 μN [1.06 mN/mm2]). Rheobase and chronaxie of the Tissue were determined as 4.45 V and 0.72 ms, respectively. These results indicate that the artificial Skeletal Muscle Tissue constructs fabricated in this study were physiologically functional and the data obtained for the evaluation of their functional properties may provide useful information for future Skeletal Muscle Tissue engineering studies.
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Fabrication of scaffold-free contractile Skeletal Muscle Tissue using magnetite-incorporated myogenic C2C12 cells.
Journal of Tissue Engineering and Regenerative Medicine, 2010Co-Authors: Hideaki Fujita, Kazunori Shimizu, Yasunori Yamamoto, Masamichi Kamihira, Eiji NagamoriAbstract:We have fabricated a functional Skeletal Muscle Tissue using magnetite-incorporated myogenic cell line C2C12 and a magnetic field. Magnetite-incorporated C2C12 cells were patterned linearly on a monolayer of fibroblast NIH3T3 cells, using a magnetic field concentrator. After induction of differentiation, the C2C12 cells fused and formed multi-nucleated myotubes. The 3T3 layer became detached in a sheet-like manner after cultivation in differentiation medium for 5–8 days. When two separate collagen films were placed on a culture dish as tendon structures, a cylindrical construct was formed. Histological observation of the fabricated cylindrical Tissue revealed the presence of multinucleate cells within it. Immunofluorescence staining of the construct showed the presence of sarcomere structures within the construct. Western blot analysis showed that Muscle proteins were expressed in the construct. When the construct was stimulated with electric pulses, it exhibited active tension of approximately 1 µN. These results demonstrate that functional Skeletal Muscle Tissue was formed through magnetic force-based Tissue engineering. This is the first report of fabrication of Skeletal Muscle Tissue with active tension-generating capability using magnetic force-based Tissue engineering. The scaffold-free Skeletal Muscle Tissue engineering technique presented in this study will be useful for regenerative medicine, drug screening or use as a bio-actuator. Copyright © 2010 John Wiley & Sons, Ltd.
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Skeletal Muscle Tissue engineering using functional magnetite nanoparticles
2009 International Symposium on Micro-NanoMechatronics and Human Science, 2009Co-Authors: Hirokazu Akiyama, Yasunori Yamamoto, Yoshinori Kawabe, Masamichi KamihiraAbstract:Skeletal muscular Tissues were constructed using magnetic force-based Tissue engineering (Mag-TE) techniques. Mouse myoblast C2C12 cells labeled with magnetite cationic liposomes (MCLs) were seeded into a well of 24-well ultra-low cell attachment culture plates. When a magnet was positioned underneath the well, cells accumulated evenly onto the culture surface and formed a multilayered cell sheet. Furthermore, because an angiogenic potential of transplants is considered to be important for the long-term maintenance of cell survival and Tissue functions, a vascular endothelial growth factor (VEGF) gene-modified C2C12 (C2C12/VEGF) cell sheets were also fabricated by the Mag-TE technique. The secretion level of C2C12/VEGF sheets was 3.0 ng/day, indicating that VEGF gene-expressing cell sheets were successfully fabricated. Since the shape of artificial Tissue constructs can be controlled by magnetic force, a cellular string-like assembly was formed by placing a linear-shaped magnetic field concentrator with a magnet. These cellular sheets and strings shrank and did not maintain their shapes for an additional in vitro culture period during myogenic differentiation. On the other hand, when a silicone plug was positioned at the center of well during the fabrication of cell sheets, the cell sheets shrank and formed a ring-like assembly around the plug. After 6-d cultivation of cell rings in differentiation medium, the C2C12 cells differentiated to form multinucleated myotubes. Thus, these procedures can provide a novel strategy for Skeletal muscular Tissue engineering.
Peng Yuan Wang - One of the best experts on this subject based on the ideXlab platform.
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grooved plga films incorporated with rgd yigsr peptides for potential application on Skeletal Muscle Tissue engineering
Colloids and Surfaces B: Biointerfaces, 2013Co-Authors: Peng Yuan Wang, Tsung Han Wu, Weibor Tsai, Mengjiy WangAbstract:Abstract Alignment of myocytes or myotubes is critical for Skeletal Muscle Tissue engineering. In this study, grooved PLGA films (800 nm in width of ridge/groove and 600 nm in depth) incorporated with RGD or YIGSR peptides were fabricated to evaluate its efficacy for Skeletal Muscle Tissue engineering. The growth and differentiation of C2C12 myoblasts were enhanced by the presentation of RGD or YIGSR compared with the untreated PLGA control. On the other hand, cell morphology was guided by the grooved structure, i.e. alignment of myoblasts and myotubes with the direction of grooves. This study elucidates the effects of both surface biochemical and topographic cues on the proliferation and differentiation of C2C12 myoblasts on biodegradable polymer films. Combination of surface topography and peptide presentation has a great potential in designing scaffolds for Skeletal Muscle Tissue engineering.
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Grooved PLGA films incorporated with RGD/YIGSR peptides for potential application on Skeletal Muscle Tissue engineering.
Colloids and Surfaces B: Biointerfaces, 2013Co-Authors: Peng Yuan Wang, Tsung Han Wu, Weibor Tsai, Mengjiy WangAbstract:Abstract Alignment of myocytes or myotubes is critical for Skeletal Muscle Tissue engineering. In this study, grooved PLGA films (800 nm in width of ridge/groove and 600 nm in depth) incorporated with RGD or YIGSR peptides were fabricated to evaluate its efficacy for Skeletal Muscle Tissue engineering. The growth and differentiation of C2C12 myoblasts were enhanced by the presentation of RGD or YIGSR compared with the untreated PLGA control. On the other hand, cell morphology was guided by the grooved structure, i.e. alignment of myoblasts and myotubes with the direction of grooves. This study elucidates the effects of both surface biochemical and topographic cues on the proliferation and differentiation of C2C12 myoblasts on biodegradable polymer films. Combination of surface topography and peptide presentation has a great potential in designing scaffolds for Skeletal Muscle Tissue engineering.