The Experts below are selected from a list of 4311 Experts worldwide ranked by ideXlab platform
Disheng Yang - One of the best experts on this subject based on the ideXlab platform.
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gelatin chondroitin 6 sulfate hyaluronic acid scaffold seeded with vascular endothelial growth factor 165 modified hair follicle stem cells as a three dimensional Skin Substitute
Stem Cell Research & Therapy, 2014Co-Authors: Renfu Quan, Xuan Zheng, Shichao Xu, Liang Zhang, Disheng YangAbstract:Introduction In the field of Skin tissue engineering, gelatin-chondroitin-6-sulfate-hyaluronic acid (Gel-C6S-HA) stents are a suitable bio Skin Substitute. The purpose was to investigate the effect of genetically-modified hair follicle stem cells (HFSCs), combined with Gel-C6S-HA scaffolds, on the vascularization of tissue-engineered Skin.
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gelatin chondroitin 6 sulfate hyaluronic acid scaffold seeded with vascular endothelial growth factor 165 modified hair follicle stem cells as a three dimensional Skin Substitute
Stem Cell Research & Therapy, 2014Co-Authors: Renfu Quan, Xuan Zheng, Liang Zhang, Disheng YangAbstract:In the field of Skin tissue engineering, gelatin-chondroitin-6-sulfate-hyaluronic acid (Gel-C6S-HA) stents are a suitable bio Skin Substitute. The purpose was to investigate the effect of genetically-modified hair follicle stem cells (HFSCs), combined with Gel-C6S-HA scaffolds, on the vascularization of tissue-engineered Skin. Three-dimensional (3D) Gel-C6S-HA scaffolds were prepared by freeze-drying. Vascular endothelial growth factor (VEGF) 165 gene-modified rat HFSCs (rHFSCs) were inoculated into the scaffolds and cultured for 7 days. Two bilateral full-thickness Skin defects were created on the back of 18 Sprague–Dawley rats. Rats were randomly divided into four groups: Group A, HFSCs transduced with VEGF165 seeded onto Gel-C6S-HA scaffolds; Group B, HFSCs transduced with empty vector seeded onto Gel-C6S-HA scaffolds; Group C, Gel-C6S-HA scaffold only; Group D, Vaseline gauze dressing. These compositions were implanted onto the defects and harvested at 7, 14 and 21 days. Wound healing was assessed and compared among groups according to hematoxylin-eosin staining, CD31 expression, alpha smooth muscle actin (α-SMA) and major histocompatibility complex class I (MHC-I) immunohistochemistry, and microvessel density (MVD) count, to evaluate the new blood vessels. SEM revealed the Gel-C6S-HA scaffold was spongy and 3D, with an average pore diameter of 133.23 ± 43.36 μm. Cells seeded on scaffolds showed good adherent growth after 7 days culture. No significant difference in rHFSC morphology, adherence and proliferative capacity was found before and after transfection (P >0.05). After 14 and 21 days, the highest rate of wound healing was observed in Group A (P <0.05). Histological and immunological examination showed that after 21 days, MVD also reached a maximum in Group A (P <0.05). Therefore, the number of new blood vessels formed within the Skin Substitutes was greatest in Group A, followed by Group B. In Group C, only trace amounts of mature subcutaneous blood vessels were observed, and few subcutaneous tissue cells migrated into the scaffolds. Tissue-engineered Skin constructs, using 3D Gel-C6S-HA scaffolds seeded with VEGF165-modified rHFSCs, resulted in promotion of angiogenesis during wound healing and facilitation of vascularization in Skin Substitutes. This may be a novel approach for tissue-engineered Skin Substitutes.
Renfu Quan - One of the best experts on this subject based on the ideXlab platform.
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gelatin chondroitin 6 sulfate hyaluronic acid scaffold seeded with vascular endothelial growth factor 165 modified hair follicle stem cells as a three dimensional Skin Substitute
Stem Cell Research & Therapy, 2014Co-Authors: Renfu Quan, Xuan Zheng, Shichao Xu, Liang Zhang, Disheng YangAbstract:Introduction In the field of Skin tissue engineering, gelatin-chondroitin-6-sulfate-hyaluronic acid (Gel-C6S-HA) stents are a suitable bio Skin Substitute. The purpose was to investigate the effect of genetically-modified hair follicle stem cells (HFSCs), combined with Gel-C6S-HA scaffolds, on the vascularization of tissue-engineered Skin.
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gelatin chondroitin 6 sulfate hyaluronic acid scaffold seeded with vascular endothelial growth factor 165 modified hair follicle stem cells as a three dimensional Skin Substitute
Stem Cell Research & Therapy, 2014Co-Authors: Renfu Quan, Xuan Zheng, Liang Zhang, Disheng YangAbstract:In the field of Skin tissue engineering, gelatin-chondroitin-6-sulfate-hyaluronic acid (Gel-C6S-HA) stents are a suitable bio Skin Substitute. The purpose was to investigate the effect of genetically-modified hair follicle stem cells (HFSCs), combined with Gel-C6S-HA scaffolds, on the vascularization of tissue-engineered Skin. Three-dimensional (3D) Gel-C6S-HA scaffolds were prepared by freeze-drying. Vascular endothelial growth factor (VEGF) 165 gene-modified rat HFSCs (rHFSCs) were inoculated into the scaffolds and cultured for 7 days. Two bilateral full-thickness Skin defects were created on the back of 18 Sprague–Dawley rats. Rats were randomly divided into four groups: Group A, HFSCs transduced with VEGF165 seeded onto Gel-C6S-HA scaffolds; Group B, HFSCs transduced with empty vector seeded onto Gel-C6S-HA scaffolds; Group C, Gel-C6S-HA scaffold only; Group D, Vaseline gauze dressing. These compositions were implanted onto the defects and harvested at 7, 14 and 21 days. Wound healing was assessed and compared among groups according to hematoxylin-eosin staining, CD31 expression, alpha smooth muscle actin (α-SMA) and major histocompatibility complex class I (MHC-I) immunohistochemistry, and microvessel density (MVD) count, to evaluate the new blood vessels. SEM revealed the Gel-C6S-HA scaffold was spongy and 3D, with an average pore diameter of 133.23 ± 43.36 μm. Cells seeded on scaffolds showed good adherent growth after 7 days culture. No significant difference in rHFSC morphology, adherence and proliferative capacity was found before and after transfection (P >0.05). After 14 and 21 days, the highest rate of wound healing was observed in Group A (P <0.05). Histological and immunological examination showed that after 21 days, MVD also reached a maximum in Group A (P <0.05). Therefore, the number of new blood vessels formed within the Skin Substitutes was greatest in Group A, followed by Group B. In Group C, only trace amounts of mature subcutaneous blood vessels were observed, and few subcutaneous tissue cells migrated into the scaffolds. Tissue-engineered Skin constructs, using 3D Gel-C6S-HA scaffolds seeded with VEGF165-modified rHFSCs, resulted in promotion of angiogenesis during wound healing and facilitation of vascularization in Skin Substitutes. This may be a novel approach for tissue-engineered Skin Substitutes.
Xuan Zheng - One of the best experts on this subject based on the ideXlab platform.
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gelatin chondroitin 6 sulfate hyaluronic acid scaffold seeded with vascular endothelial growth factor 165 modified hair follicle stem cells as a three dimensional Skin Substitute
Stem Cell Research & Therapy, 2014Co-Authors: Renfu Quan, Xuan Zheng, Shichao Xu, Liang Zhang, Disheng YangAbstract:Introduction In the field of Skin tissue engineering, gelatin-chondroitin-6-sulfate-hyaluronic acid (Gel-C6S-HA) stents are a suitable bio Skin Substitute. The purpose was to investigate the effect of genetically-modified hair follicle stem cells (HFSCs), combined with Gel-C6S-HA scaffolds, on the vascularization of tissue-engineered Skin.
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gelatin chondroitin 6 sulfate hyaluronic acid scaffold seeded with vascular endothelial growth factor 165 modified hair follicle stem cells as a three dimensional Skin Substitute
Stem Cell Research & Therapy, 2014Co-Authors: Renfu Quan, Xuan Zheng, Liang Zhang, Disheng YangAbstract:In the field of Skin tissue engineering, gelatin-chondroitin-6-sulfate-hyaluronic acid (Gel-C6S-HA) stents are a suitable bio Skin Substitute. The purpose was to investigate the effect of genetically-modified hair follicle stem cells (HFSCs), combined with Gel-C6S-HA scaffolds, on the vascularization of tissue-engineered Skin. Three-dimensional (3D) Gel-C6S-HA scaffolds were prepared by freeze-drying. Vascular endothelial growth factor (VEGF) 165 gene-modified rat HFSCs (rHFSCs) were inoculated into the scaffolds and cultured for 7 days. Two bilateral full-thickness Skin defects were created on the back of 18 Sprague–Dawley rats. Rats were randomly divided into four groups: Group A, HFSCs transduced with VEGF165 seeded onto Gel-C6S-HA scaffolds; Group B, HFSCs transduced with empty vector seeded onto Gel-C6S-HA scaffolds; Group C, Gel-C6S-HA scaffold only; Group D, Vaseline gauze dressing. These compositions were implanted onto the defects and harvested at 7, 14 and 21 days. Wound healing was assessed and compared among groups according to hematoxylin-eosin staining, CD31 expression, alpha smooth muscle actin (α-SMA) and major histocompatibility complex class I (MHC-I) immunohistochemistry, and microvessel density (MVD) count, to evaluate the new blood vessels. SEM revealed the Gel-C6S-HA scaffold was spongy and 3D, with an average pore diameter of 133.23 ± 43.36 μm. Cells seeded on scaffolds showed good adherent growth after 7 days culture. No significant difference in rHFSC morphology, adherence and proliferative capacity was found before and after transfection (P >0.05). After 14 and 21 days, the highest rate of wound healing was observed in Group A (P <0.05). Histological and immunological examination showed that after 21 days, MVD also reached a maximum in Group A (P <0.05). Therefore, the number of new blood vessels formed within the Skin Substitutes was greatest in Group A, followed by Group B. In Group C, only trace amounts of mature subcutaneous blood vessels were observed, and few subcutaneous tissue cells migrated into the scaffolds. Tissue-engineered Skin constructs, using 3D Gel-C6S-HA scaffolds seeded with VEGF165-modified rHFSCs, resulted in promotion of angiogenesis during wound healing and facilitation of vascularization in Skin Substitutes. This may be a novel approach for tissue-engineered Skin Substitutes.
Liang Zhang - One of the best experts on this subject based on the ideXlab platform.
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gelatin chondroitin 6 sulfate hyaluronic acid scaffold seeded with vascular endothelial growth factor 165 modified hair follicle stem cells as a three dimensional Skin Substitute
Stem Cell Research & Therapy, 2014Co-Authors: Renfu Quan, Xuan Zheng, Shichao Xu, Liang Zhang, Disheng YangAbstract:Introduction In the field of Skin tissue engineering, gelatin-chondroitin-6-sulfate-hyaluronic acid (Gel-C6S-HA) stents are a suitable bio Skin Substitute. The purpose was to investigate the effect of genetically-modified hair follicle stem cells (HFSCs), combined with Gel-C6S-HA scaffolds, on the vascularization of tissue-engineered Skin.
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gelatin chondroitin 6 sulfate hyaluronic acid scaffold seeded with vascular endothelial growth factor 165 modified hair follicle stem cells as a three dimensional Skin Substitute
Stem Cell Research & Therapy, 2014Co-Authors: Renfu Quan, Xuan Zheng, Liang Zhang, Disheng YangAbstract:In the field of Skin tissue engineering, gelatin-chondroitin-6-sulfate-hyaluronic acid (Gel-C6S-HA) stents are a suitable bio Skin Substitute. The purpose was to investigate the effect of genetically-modified hair follicle stem cells (HFSCs), combined with Gel-C6S-HA scaffolds, on the vascularization of tissue-engineered Skin. Three-dimensional (3D) Gel-C6S-HA scaffolds were prepared by freeze-drying. Vascular endothelial growth factor (VEGF) 165 gene-modified rat HFSCs (rHFSCs) were inoculated into the scaffolds and cultured for 7 days. Two bilateral full-thickness Skin defects were created on the back of 18 Sprague–Dawley rats. Rats were randomly divided into four groups: Group A, HFSCs transduced with VEGF165 seeded onto Gel-C6S-HA scaffolds; Group B, HFSCs transduced with empty vector seeded onto Gel-C6S-HA scaffolds; Group C, Gel-C6S-HA scaffold only; Group D, Vaseline gauze dressing. These compositions were implanted onto the defects and harvested at 7, 14 and 21 days. Wound healing was assessed and compared among groups according to hematoxylin-eosin staining, CD31 expression, alpha smooth muscle actin (α-SMA) and major histocompatibility complex class I (MHC-I) immunohistochemistry, and microvessel density (MVD) count, to evaluate the new blood vessels. SEM revealed the Gel-C6S-HA scaffold was spongy and 3D, with an average pore diameter of 133.23 ± 43.36 μm. Cells seeded on scaffolds showed good adherent growth after 7 days culture. No significant difference in rHFSC morphology, adherence and proliferative capacity was found before and after transfection (P >0.05). After 14 and 21 days, the highest rate of wound healing was observed in Group A (P <0.05). Histological and immunological examination showed that after 21 days, MVD also reached a maximum in Group A (P <0.05). Therefore, the number of new blood vessels formed within the Skin Substitutes was greatest in Group A, followed by Group B. In Group C, only trace amounts of mature subcutaneous blood vessels were observed, and few subcutaneous tissue cells migrated into the scaffolds. Tissue-engineered Skin constructs, using 3D Gel-C6S-HA scaffolds seeded with VEGF165-modified rHFSCs, resulted in promotion of angiogenesis during wound healing and facilitation of vascularization in Skin Substitutes. This may be a novel approach for tissue-engineered Skin Substitutes.
Thomas Biedermann - One of the best experts on this subject based on the ideXlab platform.
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bioengineering a prevascularized human tri layered Skin Substitute containing a hypodermis
Acta Biomaterialia, 2021Co-Authors: Jakub Zimoch, Dominika Zielinska, Katarzyna Michalakmicka, Dominic Rutsche, Roland Boni, Thomas BiedermannAbstract:Abstract Severe injuries to Skin including hypodermis require full-thickness Skin replacement. Here, we bioengineered a tri-layered human Skin Substitute (TLSS) containing the epidermis, dermis, and hypodermis. The hypodermal layer was generated by differentiation of human adipose stem cells (ASC) in a collagen type I hydrogel and combined with a prevascularized dermis consisting of human dermal microvascular endothelial cells and fibroblasts, which arranged into a dense vascular network. Subsequently, keratinocytes were seeded on top to generate the epidermal layer of the TLSS. The differentiation of ASC into adipocytes was confirmed in vitro on the mRNA level by the presence of adiponectin, as well as by the expression of perilipin and FABP-4 proteins. Moreover, functional characteristics of the hypodermis in vitro and in vivo were evaluated by Oil Red O, BODIPY, and AdipoRed stainings visualizing intracellular lipid droplets. Further, we demonstrated that both undifferentiated ASC and mature adipocytes present in the hypodermis influenced the keratinocyte maturation and homeostasis in the Skin Substitutes after transplantation. In particular, an enhanced secretion of TGF-β1 by these cells affected the epidermal morphogenesis as assessed by the expression of key proteins involved in the epidermal differentiation including cytokeratin 1, 10, 19 and cornified envelope formation such as involucrin. Here, we propose a novel functional hypodermal-dermo-epidermal tri-layered Skin Substitute containing blood capillaries that efficiently promote regeneration of Skin defects. Statement of significance The main objective of this study was to develop and assess the usefulness of a tri-layered human prevascularized Skin Substitute (TLSS) containing an epidermis, dermis, and hypodermis. The bioengineered hypodermis was generated from human adipose mesenchymal stem cells (ASC) and combined with a prevascularized dermis and epidermis. The TLSS represents an exceptional model for studying the role of cell-cell and cell-matrix interactions in vitro and in vivo. In particular, we observed that enhanced secretion of TGF-β1 in the hypodermis exerted a profound impact on fibroblast and keratinocyte differentiation, as well as epidermal barrier formation and homeostasis. Therefore, improved understanding of the cell-cell interactions in such a physiological Skin model is essential to gain insights into different aspects of wound healing.
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bio engineering a prevascularized human tri layered Skin Substitute containing a hypodermis
Social Science Research Network, 2021Co-Authors: Jakub Zimoch, Thomas Biedermann, Dominika Zielinska, Katarzyna Michalakmicka, Dominic Rutsche, Roland Boni, Agnes S KlarAbstract:Severe injuries to Skin including hypodermis require full-thickness Skin replacement. Here, we bio-engineered a tri-layered human Skin Substitute (TLSS) containing the epidermis, dermis, and hypodermis. The hypodermal layer was generated by differentiation of human adipose stem cells (ASC) in a collagen type I hydrogel and combined with a prevascularized dermis consisting of human dermal microvascular endothelial cells and fibroblasts, which arranged into a dense vascular network. Subsequently, keratinocytes were seeded on top to generate the epidermal layer of the TLSS. The differentiation of ASC into adipocytes was confirmed in vitro on the mRNA level by the presence of adiponectin, and by the expression of perilipin and FABP-4 proteins. Moreover, functional characteristics of the hypodermis in vitro and in vivo were evaluated by Oil Red, BODIPY, and AdipoRed stainings visualizing intracellular lipid droplets. Further, the enhanced secretion of TGF-β1 by hypodermal layer affected the epidermal morphogenesis as assessed by the expression of key proteins involved in the epidermal differentiation - cytokeratin 1, 10, 19, markers for cornified envelope formation -involucrin, and components of dermo-epidermal junction – laminin 332 in vivo. Here, we propose a novel functional hypodermal-dermo-epidermal tri-layered Skin Substitute containing blood capillaries that efficiently promote regeneration of Skin defects.
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The Effect of Wound Dressings on a Bio-Engineered Human Dermo-Epidermal Skin Substitute in a Rat Model.
Journal of burn care & research : official publication of the American Burn Association, 2017Co-Authors: Martina Hüging, Thomas Biedermann, Monia Sobrio, Sarah A. Meyer, Sophie Böttcher-haberzeth, Edith Manuel, Maya Horst, Sally Hynes, Ernst Reichmann, Clemens SchiestlAbstract:Autologous bio-engineered dermo-epidermal Skin Substitutes are a promising treatment for large Skin defects such as burns. For their successful clinical application, the graft dressing must protect and support the keratinocyte layer and, in many cases, possess antimicrobial properties. However, silver in many antimicrobial dressings may inhibit keratinocyte growth and differentiation. The purpose of our study was to evaluate the effect of various wound dressings on the healing of a human hydrogel-based dermo-epidermal Skin Substitute in preparation for the first-in-human clinical trials. Human dermo-epidermal Skin Substitutes approved for clinical trials were produced under good manufacturing practice conditions, transplanted onto immuno-incompetent rats, and dressed with either Vaseline Gauze™ (Kendall Medtronic, Minneapolis, USA), Suprathel (PolyMedics Innovations GmbH, Denkendorf, Germany), Urgotul SSD (Urgo Medical, Shepshed, United Kingdom), Mepilex AG (Molnlycke Health Care, Gothenburg, Sweden), or Acticoat™ (Smith&Nephew, Baar, Switzerland). Grafts were assessed clinically for take, epithelialization, and infection at 10 and 21 days post-transplantation, and histologically at 21 days. There were three subjects each in the Vaseline Gauze™ and Suprathel groups, and four subjects each in the Urgotul SSD, Mepilex AG, and Acticoat™ groups. For all samples, the take rate was 100% and the expected keratinocyte number, epithelialization and epidermal stratification were observed. All of the dressings in the current study were well tolerated by our human dermo-epidermal Skin Substitute. The tolerance of the silver-based dressings is particularly relevant given the high risk of bacterial contamination with large Skin defects, and provides pivotal information as we embark on clinical trials for this novel Skin Substitute.
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tissue engineering of Skin for wound coverage
European Journal of Pediatric Surgery, 2013Co-Authors: Thomas Biedermann, Sophie Boettcherhaberzeth, Ernst ReichmannAbstract:Over the past few decades, important milestones have been reached in the field of Skin tissue engineering, bringing the ultimate goal of fabricating an autologous dermoepidermal Skin Substitute with all its cellular components and Skin appendages closer to reality. Yet, scientific progress alone is not enough, clinical demands must be addressed and commercial interests need to be fulfilled. This review gives an overview of commercially available Skin Substitutes for Skin replacement therapies and an insight into the recent development of an autologous full-thickness Skin Substitute that can readily be transplanted in large quantities onto the patient.
