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Alberto Carlos Botazzo Delbem - One of the best experts on this subject based on the ideXlab platform.
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effect of fluoride casein phosphopeptide amorphous calcium phosphate and Sodium Trimetaphosphate combination treatment on the remineralization of caries lesions an in vitro study
Archives of Oral Biology, 2020Co-Authors: Francyenne Maira Castro Gonçalves, Alberto Carlos Botazzo Delbem, Leonardo Fernandes Gomes, Nayara Gonçalves Emerenciano, Mark L. Cannon, Juliano Pelim Pessan, Giovana Dornelas Azevedo Romero, Marcelle DanelonAbstract:Abstract Objective To evaluate the effects of combination of treatments with fluoridated toothpastes supplemented with Sodium Trimetaphosphate (TMP) and casein phosphopeptide-amorphous calcium phosphate (MI Paste Plus®), on the remineralization of dental enamel. Design Enamel blocks with artificial caries were randomly allocated into six groups (n = 12), according to the toothpastes: 1) without F-TMP-MI Paste Plus® (Placebo); 2) 1100 ppm F (1100 F), 3) MI Paste Plus®, 4) 1100 F + MI Paste Plus® (1100 F-MI Paste Plus®), 5) 1100 F + 3% TMP (1100 F-TMP) and 6) 1100 F-TMP + MI Paste Plus® (1100 F-TMP-MI Paste Plus®). Blocks were treated 2×/day with slurries of toothpastes (1 min). Furthermore, groups 4 and 6 received the application of MI Paste Plus® for 3 min. After pH cycling, the percentage of surface hardness recovery (%SHR); integrated loss of subsurface hardness (ΔKHN); profile analysis and lesion depth subsurface through polarized light microscopy (PLM), confocal laser scanning microscopy (LSCM), scanning electron microscopy (SEM), fluoride (F), calcium (Ca), phosphorus (P) concentrations in the enamel were determined. The data were analyzed by ANOVA (1-criterion) and Student-Newman-Keuls test (p Results 1100 F-TMP-MI Paste Plus® group showed the best results of %SHR, ΔKHN and PLM (p 0.001). 1100 F-TMP-MI Paste Plus® group showed the highest concentration of Ca and P in the enamel (p Conclusion The association of 1100 F-TMP and MI Paste Plus® led to a significant increase in the remineralization of initial carious lesions.
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Combined effect of casein phosphopeptide-amorphous calcium phosphate and Sodium Trimetaphosphate on the prevention of enamel demineralization and dental caries: an in vitro study.
Clinical oral investigations, 2020Co-Authors: Francyenne Maira Castro Gonçalves, Alberto Carlos Botazzo Delbem, Leonardo Fernandes Gomes, Matheus Dos Passos Silva, Mark Cannon, Marcelle DanelonAbstract:OBJECTIVE To evaluate the effect of treatment with fluoridated toothpaste supplemented with a combination of Sodium Trimetaphosphate (TMP) and casein phosphopeptide-amorphous calcium phosphate (MI Paste Plus®) on the demineralization of dental enamel. METHODS Bovine enamel blocks selected by initial surface hardness (SHi) were randomly allocated into six groups (n = 12), according to the test toothpastes: (1) without F-TMP-MI Paste Plus® (Placebo); (2) 1100 ppm F (1100F); (3) MI Paste Plus®; (4) 1100F + MI Paste Plus® (1100F-MI Paste Plus), (5) 1100F + 3 % TMP (1100F-TMP); and (6) 1100F-TMP + MI Paste Plus® (1100F-TMP-MI Paste Plus). Blocks were treated two times per day with slurries of toothpaste (1 min), and groups 4 and 6 received an application of MI Paste Plus (3 min). Next, the samples were subjected to five pH cycles (demineralizing/remineralizing solutions) at 37 °C, to produce subsurface enamel lesions.Thereafter, the blocks were maintained for 2 days in fresh remineralizing solution. After pH cycling, the following were obtained: percentage of surface hardness loss (%SH); integrated loss of subsurface hardness (ΔKHN); profile analysis and lesion depth subsurface through polarized light microscopy (PLM); scanning electron microscopy (SEM); and fluoride (F), calcium (Ca), and phosphorus (P) in the enamel. The data were subjected to ANOVA (1-criterion), followed by the Student-Newman-Keuls test (p 0.001). The 1100F-TMP-MI Paste Plus group showed the highest concentration of Ca and P in the enamel (p < 0.001). CONCLUSION The application of 1100F-TMP-MI Paste Plus promoted a higher inhibitory effect against enamel demineralization. CLINICAL SIGNIFICANCE The combination of treatments with F, TMP, and MI Paste Plus® can be an effective alternative to improve the oral health of individuals, especially those with high activity of dental caries and at high risk for its development.
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Effects of Sodium Trimetaphosphate, Associated or Not with Fluoride, on the Composition and pH of Mixed Biofilms, before and after Exposure to Sucrose.
Caries research, 2020Co-Authors: Thamires Priscila Cavazana, Juliano Pelim Pessan, Ty Hosida, Sampaio C, Douglas Roberto Monteiro, Viviane De Oliveira Zequini Amarante, Alberto Carlos Botazzo DelbemAbstract:The aim of the present study was to evaluate the influence of Sodium Trimetaphosphate (TMP), associated or not with fluoride (F), on the concentrations of F, calcium (Ca), and phosphorus (P) and on the pH of mixed biofilms of Streptococcus mutans and Candida albicans, before and after exposure to sucrose. The biofilms received three treatments (72, 78, and 96 h after the beginning of their formation), at three TMP concentrations (0.25, 0.5, or 1%), with or without F at 500 ppm. Solutions containing 500 and 1,100 ppm F as well as artificial saliva were also tested as controls. Biofilm pH was measured and the concentrations of F, Ca, and P were determined (solid and fluid phases). In a parallel experiment, after the third treatment (96 h), the biofilms were exposed to a 20% sucrose solution to simulate a cariogenic challenge and the pH of the medium, F, Ca, P, and TMP were determined. The data were submitted by two-way ANOVA, followed by Fisher's least significant difference test (p < 0.05). Treatment with TMP and 500 ppm F led to higher F concentration in the biofilm fluid. Although TMP did not affect Ca concentrations, biofilms treated with TMP alone presented higher P concentrations. Treatment with 1% TMP and F led to the highest pH values of the biofilm, both before and after the cariogenic challenge. It was concluded that TMP increases F and P in the biofilm and that its presence promotes an increase in the pH of the medium, even after the cariogenic challenge.
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Combined effect of casein phosphopeptide-amorphous calcium phosphate and Sodium Trimetaphosphate on the prevention of enamel demineralization and dental caries: an in vitro study
Clinical Oral Investigations, 2020Co-Authors: Francyenne Maira Castro Gonçalves, Alberto Carlos Botazzo Delbem, Leonardo Fernandes Gomes, Nayara Gonçalves Emerenciano, Matheus Passos Silva, Mark L. Cannon, Marcelle DanelonAbstract:Objective To evaluate the effect of treatment with fluoridated toothpaste supplemented with a combination of Sodium Trimetaphosphate (TMP) and casein phosphopeptide-amorphous calcium phosphate (MI Paste Plus^®) on the demineralization of dental enamel. Methods Bovine enamel blocks selected by initial surface hardness (SHi) were randomly allocated into six groups ( n = 12), according to the test toothpastes: (1) without F-TMP-MI Paste Plus^® (Placebo); (2) 1100 ppm F (1100F); (3) MI Paste Plus^®; (4) 1100F + MI Paste Plus^® (1100F-MI Paste Plus), (5) 1100F + 3 % TMP (1100F-TMP); and (6) 1100F-TMP + MI Paste Plus^® (1100F-TMP-MI Paste Plus). Blocks were treated two times per day with slurries of toothpaste (1 min), and groups 4 and 6 received an application of MI Paste Plus (3 min). Next, the samples were subjected to five pH cycles (demineralizing/remineralizing solutions) at 37 °C, to produce subsurface enamel lesions.Thereafter, the blocks were maintained for 2 days in fresh remineralizing solution. After pH cycling, the following were obtained: percentage of surface hardness loss (%SH); integrated loss of subsurface hardness (ΔKHN); profile analysis and lesion depth subsurface through polarized light microscopy (PLM); scanning electron microscopy (SEM); and fluoride (F), calcium (Ca), and phosphorus (P) in the enamel. The data were subjected to ANOVA (1-criterion), followed by the Student–Newman–Keuls test ( p < 0.001). Results The 1100F-TMP-MI Paste Plus group showed better results for SHR, ΔKHN, and PLM ( p < 0.001). The F concentration was similar among all groups ( p > 0.001). The 1100F-TMP-MI Paste Plus group showed the highest concentration of Ca and P in the enamel ( p < 0.001). Conclusion The application of 1100F-TMP-MI Paste Plus promoted a higher inhibitory effect against enamel demineralization. Clinical significance The combination of treatments with F, TMP, and MI Paste Plus^® can be an effective alternative to improve the oral health of individuals, especially those with high activity of dental caries and at high risk for its development.
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Hydraulic conductance of dentin after treatment with fluoride toothpaste containing Sodium Trimetaphosphate microparticles or nanoparticles
Clinical Oral Investigations, 2020Co-Authors: Carla Oliveira Favretto, Priscila Toninatto Alves Toledo, Alberto Carlos Botazzo Delbem, Denise PedriniAbstract:Objectives To evaluate the hydraulic conductance of dentin after treatment with fluoride toothpastes containing Sodium Trimetaphosphate microparticles (TMPmicro) or nanoparticles (TMPnano). Materials and methods The dentinal tubules of bovine dentin blocks (4 × 4 × 1 mm) were unobstructed for determination of the maximum hydraulic conductance of the dentin. The dentin blocks were randomized into four groups ( n = 15/group) of toothpastes (placebo, 1100 ppm F, and 1100 with 3% TMPmicro or 3% TMPnano) which were applied for 7 days (2×/day) using a brushing machine. The dentin surface (5/group) was analyzed by scanning electron microscopy. The hydraulic conductance post-treatment was measured in the other ten blocks. Thereafter, the same blocks were immersed in citric acid (pH 3.2) for 1 min, and the conductance was determined again. The data were submitted to 2-way ANOVA repeated measures, followed by the Student-Newman-Keuls test ( p < 0.05). Results The percentage conductance reduction post-treatment for the groups were placebo = 1100 ppm F < 1100 TMPnano < 1100 TMPmicro ( p < 0.001). After acid attack, the percentage reduction was placebo < 1100 ppm F < 1100 TMPnano < 1100 TMPmicro ( p < 0.001). The toothpastes containing TMP showed the highest obliteration of dentinal tubules. Conclusions The addition of TMPmicro to fluoride toothpaste produced a greater reduction in hydraulic conductance when compared with 1100 ppm F toothpaste. Clinical relevance The increased capacity of toothpastes containing TMP to reduce hydraulic conductance indicates their potential to reduce symptoms of dentinal hypersensitivity.
Denise Pedrini - One of the best experts on this subject based on the ideXlab platform.
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Hydraulic conductance of dentin after treatment with fluoride toothpaste containing Sodium Trimetaphosphate microparticles or nanoparticles
Clinical Oral Investigations, 2020Co-Authors: Carla Oliveira Favretto, Priscila Toninatto Alves Toledo, Alberto Carlos Botazzo Delbem, Denise PedriniAbstract:Objectives To evaluate the hydraulic conductance of dentin after treatment with fluoride toothpastes containing Sodium Trimetaphosphate microparticles (TMPmicro) or nanoparticles (TMPnano). Materials and methods The dentinal tubules of bovine dentin blocks (4 × 4 × 1 mm) were unobstructed for determination of the maximum hydraulic conductance of the dentin. The dentin blocks were randomized into four groups ( n = 15/group) of toothpastes (placebo, 1100 ppm F, and 1100 with 3% TMPmicro or 3% TMPnano) which were applied for 7 days (2×/day) using a brushing machine. The dentin surface (5/group) was analyzed by scanning electron microscopy. The hydraulic conductance post-treatment was measured in the other ten blocks. Thereafter, the same blocks were immersed in citric acid (pH 3.2) for 1 min, and the conductance was determined again. The data were submitted to 2-way ANOVA repeated measures, followed by the Student-Newman-Keuls test ( p < 0.05). Results The percentage conductance reduction post-treatment for the groups were placebo = 1100 ppm F < 1100 TMPnano < 1100 TMPmicro ( p < 0.001). After acid attack, the percentage reduction was placebo < 1100 ppm F < 1100 TMPnano < 1100 TMPmicro ( p < 0.001). The toothpastes containing TMP showed the highest obliteration of dentinal tubules. Conclusions The addition of TMPmicro to fluoride toothpaste produced a greater reduction in hydraulic conductance when compared with 1100 ppm F toothpaste. Clinical relevance The increased capacity of toothpastes containing TMP to reduce hydraulic conductance indicates their potential to reduce symptoms of dentinal hypersensitivity.
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Incorporation of chlorhexidine and nano-sized Sodium Trimetaphosphate into a glass-ionomer cement: Effect on mechanical and microbiological properties and inhibition of enamel demineralization
Journal of dentistry, 2019Co-Authors: Marjully Eduardo Rodrigues Da Silva, Denise Pedrini, Alberto Carlos Botazzo Delbem, Marcelle Danelon, José Antonio Santos Souza, Emerson R Camargo, Dinah Fressato Silva, Jesse Augusto Pereira, Cristiane DuqueAbstract:Abstract Objective To evaluate the antimicrobial/antibiofilm and mechanical properties, and the effect on enamel demineralization of a resin-modified GIC (RMGIC) containing CHX and nano-sized Sodium Trimetaphosphate (TMP). Methods RMGIC was associated with CHX (1.25 or 2.5%) and/or TMP (7 or 14%). Antimicrobial and antibiofilm activity were assessed using agar diffusion test and evaluation of biofilm metabolism, respectively. In addition, fluoride (F) and TMP releases as well as the diametral tensile (DTS) and compressive (CS) strength were determined. The percentage of mineral loss (%SH), integrated loss of subsurface hardness (ΔKHN) and enamel F concentrations were also evaluated. Results RMGICs containing CHX associated or not with TMP presented higher inhibition zones and effect on S. mutans biofilm. A reduction on CS was observed only for RMGIC + 2.5%CHX and on DTS for RMGIC + 2.5%CHX + 14%TMP. The highest F and TMP releases and lowest %SH and ΔKHN values were detected for RMGIC + 1.25%CHX + 14%TMP and RMGIC + 2.5%CHX + 14%TMP. Higher enamel F concentrations were observed for TMP groups. Conclusion 1.25%CHX and 14%TMP increased antimicrobial/antibiofilm action and the ability to prevent enamel demineralization, with minimal effect on the mechanical properties of RMGIC. Clinical significance RMGIC containing CHX and TMP is an alternative material for patients at high risk for dental caries and can be indicated for low-stress regions or provisional restorations.
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In vitro enamel remineralization capacity of composite resins containing Sodium Trimetaphosphate and fluoride
Clinical Oral Investigations, 2015Co-Authors: Adelisa Rodolfo Ferreira Tiveron, Kikue Takebayashi Sassaki, Alberto Carlos Botazzo Delbem, Gabriel Gaban, Denise PedriniAbstract:Objectives This study evaluated the in vitro enamel remineralization capacity of experimental composite resins containing Sodium Trimetaphosphate (TMP) combined or not with fluoride (F). Materials and methods Bovine enamel slabs were selected upon analysis of initial surface hardness (SH_1) and after induction of artificial carious lesions (SH_2). Experimental resins were as follows: resin C (control—no Sodium fluoride (NaF) or TMP), resin F (with 1.6 % NaF), resin TMP (with 14.1 % TMP), and resin TMP/F (with NaF and TMP). Resin samples were made and attached to enamel slabs ( n = 12 slabs per material). Those specimens (resin/enamel slab) were subjected to pH cycling to promote remineralization, and then final surface hardness (SH_3) was measured to calculate the percentage of surface hardness recovery (%SH). The integrated recovery of subsurface hardness (ΔKHN) and F concentration in enamel were also determined. Data was analyzed by ANOVA and Student–Newman–Keuls test ( p
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in vitro enamel remineralization capacity of composite resins containing Sodium Trimetaphosphate and fluoride
Clinical Oral Investigations, 2015Co-Authors: Adelisa Rodolfo Ferreira Tiveron, Kikue Takebayashi Sassaki, Alberto Carlos Botazzo Delbem, Gabriel Gaban, Denise PedriniAbstract:This study evaluated the in vitro enamel remineralization capacity of experimental composite resins containing Sodium Trimetaphosphate (TMP) combined or not with fluoride (F). Bovine enamel slabs were selected upon analysis of initial surface hardness (SH1) and after induction of artificial carious lesions (SH2). Experimental resins were as follows: resin C (control—no Sodium fluoride (NaF) or TMP), resin F (with 1.6 % NaF), resin TMP (with 14.1 % TMP), and resin TMP/F (with NaF and TMP). Resin samples were made and attached to enamel slabs (n = 12 slabs per material). Those specimens (resin/enamel slab) were subjected to pH cycling to promote remineralization, and then final surface hardness (SH3) was measured to calculate the percentage of surface hardness recovery (%SH). The integrated recovery of subsurface hardness (ΔKHN) and F concentration in enamel were also determined. Data was analyzed by ANOVA and Student–Newman–Keuls test (p < 0.05). Resins F and TMP/F showed similar SH3 values (p = 0.478) and %SH (p = 0.336) and differed significantly from the other resins (p < 0.001). Considering ΔKHN values, resin TMP/F presented the lowest area of lesion (p < 0.001). The presence of F on enamel was different among the fluoridated resins (p = 0.042), but higher than in the other resins (p < 0.001). The addition of TMP to a fluoridated composite resin enhanced its capacity for remineralization of enamel in vitro. The combination of two agents with action on enamel favored remineralization, suggesting that composite resins containing Sodium Trimetaphosphate and fluoride could be indicated for clinical procedures in situations with higher cariogenic challenges.
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In vitro enamel remineralization capacity of composite resins containing Sodium Trimetaphosphate and fluoride
Clinical oral investigations, 2015Co-Authors: Adelisa Rodolfo Ferreira Tiveron, Kikue Takebayashi Sassaki, Alberto Carlos Botazzo Delbem, Gabriel Gaban, Denise PedriniAbstract:This study evaluated the in vitro enamel remineralization capacity of experimental composite resins containing Sodium Trimetaphosphate (TMP) combined or not with fluoride (F). Bovine enamel slabs were selected upon analysis of initial surface hardness (SH1) and after induction of artificial carious lesions (SH2). Experimental resins were as follows: resin C (control—no Sodium fluoride (NaF) or TMP), resin F (with 1.6 % NaF), resin TMP (with 14.1 % TMP), and resin TMP/F (with NaF and TMP). Resin samples were made and attached to enamel slabs (n = 12 slabs per material). Those specimens (resin/enamel slab) were subjected to pH cycling to promote remineralization, and then final surface hardness (SH3) was measured to calculate the percentage of surface hardness recovery (%SH). The integrated recovery of subsurface hardness (ΔKHN) and F concentration in enamel were also determined. Data was analyzed by ANOVA and Student–Newman–Keuls test (p
Juliano Pelim Pessan - One of the best experts on this subject based on the ideXlab platform.
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effect of fluoride casein phosphopeptide amorphous calcium phosphate and Sodium Trimetaphosphate combination treatment on the remineralization of caries lesions an in vitro study
Archives of Oral Biology, 2020Co-Authors: Francyenne Maira Castro Gonçalves, Alberto Carlos Botazzo Delbem, Leonardo Fernandes Gomes, Nayara Gonçalves Emerenciano, Mark L. Cannon, Juliano Pelim Pessan, Giovana Dornelas Azevedo Romero, Marcelle DanelonAbstract:Abstract Objective To evaluate the effects of combination of treatments with fluoridated toothpastes supplemented with Sodium Trimetaphosphate (TMP) and casein phosphopeptide-amorphous calcium phosphate (MI Paste Plus®), on the remineralization of dental enamel. Design Enamel blocks with artificial caries were randomly allocated into six groups (n = 12), according to the toothpastes: 1) without F-TMP-MI Paste Plus® (Placebo); 2) 1100 ppm F (1100 F), 3) MI Paste Plus®, 4) 1100 F + MI Paste Plus® (1100 F-MI Paste Plus®), 5) 1100 F + 3% TMP (1100 F-TMP) and 6) 1100 F-TMP + MI Paste Plus® (1100 F-TMP-MI Paste Plus®). Blocks were treated 2×/day with slurries of toothpastes (1 min). Furthermore, groups 4 and 6 received the application of MI Paste Plus® for 3 min. After pH cycling, the percentage of surface hardness recovery (%SHR); integrated loss of subsurface hardness (ΔKHN); profile analysis and lesion depth subsurface through polarized light microscopy (PLM), confocal laser scanning microscopy (LSCM), scanning electron microscopy (SEM), fluoride (F), calcium (Ca), phosphorus (P) concentrations in the enamel were determined. The data were analyzed by ANOVA (1-criterion) and Student-Newman-Keuls test (p Results 1100 F-TMP-MI Paste Plus® group showed the best results of %SHR, ΔKHN and PLM (p 0.001). 1100 F-TMP-MI Paste Plus® group showed the highest concentration of Ca and P in the enamel (p Conclusion The association of 1100 F-TMP and MI Paste Plus® led to a significant increase in the remineralization of initial carious lesions.
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Effects of Sodium Trimetaphosphate, Associated or Not with Fluoride, on the Composition and pH of Mixed Biofilms, before and after Exposure to Sucrose.
Caries research, 2020Co-Authors: Thamires Priscila Cavazana, Juliano Pelim Pessan, Ty Hosida, Sampaio C, Douglas Roberto Monteiro, Viviane De Oliveira Zequini Amarante, Alberto Carlos Botazzo DelbemAbstract:The aim of the present study was to evaluate the influence of Sodium Trimetaphosphate (TMP), associated or not with fluoride (F), on the concentrations of F, calcium (Ca), and phosphorus (P) and on the pH of mixed biofilms of Streptococcus mutans and Candida albicans, before and after exposure to sucrose. The biofilms received three treatments (72, 78, and 96 h after the beginning of their formation), at three TMP concentrations (0.25, 0.5, or 1%), with or without F at 500 ppm. Solutions containing 500 and 1,100 ppm F as well as artificial saliva were also tested as controls. Biofilm pH was measured and the concentrations of F, Ca, and P were determined (solid and fluid phases). In a parallel experiment, after the third treatment (96 h), the biofilms were exposed to a 20% sucrose solution to simulate a cariogenic challenge and the pH of the medium, F, Ca, P, and TMP were determined. The data were submitted by two-way ANOVA, followed by Fisher's least significant difference test (p < 0.05). Treatment with TMP and 500 ppm F led to higher F concentration in the biofilm fluid. Although TMP did not affect Ca concentrations, biofilms treated with TMP alone presented higher P concentrations. Treatment with 1% TMP and F led to the highest pH values of the biofilm, both before and after the cariogenic challenge. It was concluded that TMP increases F and P in the biofilm and that its presence promotes an increase in the pH of the medium, even after the cariogenic challenge.
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Activity of Sodium Trimetaphosphate, associated or not with fluoride, on dual-species biofilms.
Biofouling, 2019Co-Authors: Thamires Priscila Cavazana, Juliano Pelim Pessan, Ty Hosida, Sampaio C, Douglas Roberto Monteiro, Alberto Carlos Botazzo DelbemAbstract:This study aimed to evaluate the effect of Sodium Trimetaphosphate (TMP), either with fluoride (F) or without fluoride, on dual-species biofilms of Streptococcus mutans and Candida albicans. The 72...
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In situ effect of fluoride toothpaste supplemented with nano-sized Sodium Trimetaphosphate on enamel demineralization prevention and biofilm composition.
Archives of oral biology, 2018Co-Authors: Alberto Carlos Botazzo Delbem, Juliano Pelim Pessan, Emerson R Camargo, Gabriel Pereira Nunes, Francisco Nunes De Souza Neto, Marcelle DanelonAbstract:Abstract Objective To evaluate the effect of a fluoride toothpaste containing nano-sized Sodium Trimetaphosphate (TMPnano) on enamel demineralization in situ and composition of the biofilm. Design This crossover double-blind study consisted of four phases (seven days each) and 12 volunteers who wore oral appliances containing four enamel bovine blocks. The cariogenic challenge was performed by 30% sucrose solution (6x/day). The toothpaste treatments (3x/day) were as follows: no F/TMP/TMPnano (Placebo), 1100 ppm F (1100F), 1100F plus 3% micrometric or nano-sized TMP (1100F/TMP; 1100F/TMPnano). Percentage of surface hardness loss (%SH), and integrated loss of subsurface hardness (ΔKHN), as well as enamel calcium (Ca), phosphorus (P), and fluoride (F) were determined. Moreover, biofilm formed on the blocks were analyzed for F, Ca, P, and insoluble extracellular polysaccharide (EPS) concentrations. Data were analyzed using one-way ANOVA, repeated measures followed by Fisher LSD test (p Results 1100F/TMPnano promoted the lowest %SH and ΔKHN among all groups (p Conclusion 1100F/TMPnano promoted a greater protective effect against enamel demineralization and significantly affected the composition of biofilm formed in situ when compared to 1100F toothpaste.
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fluoride toothpastes containing micrometric or nano sized Sodium Trimetaphosphate reduce enamel erosion in vitro
Acta Odontologica Scandinavica, 2018Co-Authors: Marcelle Danelon, Juliano Pelim Pessan, Emerson R Camargo, Vinícius Dos Santos, Erika Kiyoko Chiba, Luhana Santos Gonzales Garcia, Alberto Carlos Botazzo DelbemAbstract:AbstractObjective: To evaluate the effect of fluoride toothpastes supplemented with micrometric or nano-sized Sodium Trimetaphosphate (TMP or TMPnano, respectively) on enamel erosion in vitro, as well as the influence of salivary acquired pellicle and saliva.Material and methods: Bovine enamel blocks (n = 120) were randomly assigned into the following experimental toothpastes: no F/TMP/TMPnano (Placebo); 1100 ppm F (1100 ppm F); 1100 ppm F plus 3% TMP or 3% TMPnano (1100 TMP or 1100 TMPnano, respectively) and 5000 ppm F (5000 ppm F). Erosive challenge was performed by immersion of the blocks in citric acid for 5 min, followed by 2 h immersion in human or artificial saliva, 4×/day, during 5 days. After each erosive challenge, blocks were exposed to slurries of the toothpastes. Enamel erosion (µm), surface hardness (SHf) and cross-sectional hardness (ΔKHN) were analyzed as response variables and the data were submitted to two-way ANOVA, followed by the Student–Newman–Keuls test (p < .05).Results: 1100 TMPna...
Marcelle Danelon - One of the best experts on this subject based on the ideXlab platform.
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effect of fluoride casein phosphopeptide amorphous calcium phosphate and Sodium Trimetaphosphate combination treatment on the remineralization of caries lesions an in vitro study
Archives of Oral Biology, 2020Co-Authors: Francyenne Maira Castro Gonçalves, Alberto Carlos Botazzo Delbem, Leonardo Fernandes Gomes, Nayara Gonçalves Emerenciano, Mark L. Cannon, Juliano Pelim Pessan, Giovana Dornelas Azevedo Romero, Marcelle DanelonAbstract:Abstract Objective To evaluate the effects of combination of treatments with fluoridated toothpastes supplemented with Sodium Trimetaphosphate (TMP) and casein phosphopeptide-amorphous calcium phosphate (MI Paste Plus®), on the remineralization of dental enamel. Design Enamel blocks with artificial caries were randomly allocated into six groups (n = 12), according to the toothpastes: 1) without F-TMP-MI Paste Plus® (Placebo); 2) 1100 ppm F (1100 F), 3) MI Paste Plus®, 4) 1100 F + MI Paste Plus® (1100 F-MI Paste Plus®), 5) 1100 F + 3% TMP (1100 F-TMP) and 6) 1100 F-TMP + MI Paste Plus® (1100 F-TMP-MI Paste Plus®). Blocks were treated 2×/day with slurries of toothpastes (1 min). Furthermore, groups 4 and 6 received the application of MI Paste Plus® for 3 min. After pH cycling, the percentage of surface hardness recovery (%SHR); integrated loss of subsurface hardness (ΔKHN); profile analysis and lesion depth subsurface through polarized light microscopy (PLM), confocal laser scanning microscopy (LSCM), scanning electron microscopy (SEM), fluoride (F), calcium (Ca), phosphorus (P) concentrations in the enamel were determined. The data were analyzed by ANOVA (1-criterion) and Student-Newman-Keuls test (p Results 1100 F-TMP-MI Paste Plus® group showed the best results of %SHR, ΔKHN and PLM (p 0.001). 1100 F-TMP-MI Paste Plus® group showed the highest concentration of Ca and P in the enamel (p Conclusion The association of 1100 F-TMP and MI Paste Plus® led to a significant increase in the remineralization of initial carious lesions.
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Combined effect of casein phosphopeptide-amorphous calcium phosphate and Sodium Trimetaphosphate on the prevention of enamel demineralization and dental caries: an in vitro study
Clinical Oral Investigations, 2020Co-Authors: Francyenne Maira Castro Gonçalves, Alberto Carlos Botazzo Delbem, Leonardo Fernandes Gomes, Nayara Gonçalves Emerenciano, Matheus Passos Silva, Mark L. Cannon, Marcelle DanelonAbstract:Objective To evaluate the effect of treatment with fluoridated toothpaste supplemented with a combination of Sodium Trimetaphosphate (TMP) and casein phosphopeptide-amorphous calcium phosphate (MI Paste Plus^®) on the demineralization of dental enamel. Methods Bovine enamel blocks selected by initial surface hardness (SHi) were randomly allocated into six groups ( n = 12), according to the test toothpastes: (1) without F-TMP-MI Paste Plus^® (Placebo); (2) 1100 ppm F (1100F); (3) MI Paste Plus^®; (4) 1100F + MI Paste Plus^® (1100F-MI Paste Plus), (5) 1100F + 3 % TMP (1100F-TMP); and (6) 1100F-TMP + MI Paste Plus^® (1100F-TMP-MI Paste Plus). Blocks were treated two times per day with slurries of toothpaste (1 min), and groups 4 and 6 received an application of MI Paste Plus (3 min). Next, the samples were subjected to five pH cycles (demineralizing/remineralizing solutions) at 37 °C, to produce subsurface enamel lesions.Thereafter, the blocks were maintained for 2 days in fresh remineralizing solution. After pH cycling, the following were obtained: percentage of surface hardness loss (%SH); integrated loss of subsurface hardness (ΔKHN); profile analysis and lesion depth subsurface through polarized light microscopy (PLM); scanning electron microscopy (SEM); and fluoride (F), calcium (Ca), and phosphorus (P) in the enamel. The data were subjected to ANOVA (1-criterion), followed by the Student–Newman–Keuls test ( p < 0.001). Results The 1100F-TMP-MI Paste Plus group showed better results for SHR, ΔKHN, and PLM ( p < 0.001). The F concentration was similar among all groups ( p > 0.001). The 1100F-TMP-MI Paste Plus group showed the highest concentration of Ca and P in the enamel ( p < 0.001). Conclusion The application of 1100F-TMP-MI Paste Plus promoted a higher inhibitory effect against enamel demineralization. Clinical significance The combination of treatments with F, TMP, and MI Paste Plus^® can be an effective alternative to improve the oral health of individuals, especially those with high activity of dental caries and at high risk for its development.
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Combined effect of casein phosphopeptide-amorphous calcium phosphate and Sodium Trimetaphosphate on the prevention of enamel demineralization and dental caries: an in vitro study.
Clinical oral investigations, 2020Co-Authors: Francyenne Maira Castro Gonçalves, Alberto Carlos Botazzo Delbem, Leonardo Fernandes Gomes, Matheus Dos Passos Silva, Mark Cannon, Marcelle DanelonAbstract:OBJECTIVE To evaluate the effect of treatment with fluoridated toothpaste supplemented with a combination of Sodium Trimetaphosphate (TMP) and casein phosphopeptide-amorphous calcium phosphate (MI Paste Plus®) on the demineralization of dental enamel. METHODS Bovine enamel blocks selected by initial surface hardness (SHi) were randomly allocated into six groups (n = 12), according to the test toothpastes: (1) without F-TMP-MI Paste Plus® (Placebo); (2) 1100 ppm F (1100F); (3) MI Paste Plus®; (4) 1100F + MI Paste Plus® (1100F-MI Paste Plus), (5) 1100F + 3 % TMP (1100F-TMP); and (6) 1100F-TMP + MI Paste Plus® (1100F-TMP-MI Paste Plus). Blocks were treated two times per day with slurries of toothpaste (1 min), and groups 4 and 6 received an application of MI Paste Plus (3 min). Next, the samples were subjected to five pH cycles (demineralizing/remineralizing solutions) at 37 °C, to produce subsurface enamel lesions.Thereafter, the blocks were maintained for 2 days in fresh remineralizing solution. After pH cycling, the following were obtained: percentage of surface hardness loss (%SH); integrated loss of subsurface hardness (ΔKHN); profile analysis and lesion depth subsurface through polarized light microscopy (PLM); scanning electron microscopy (SEM); and fluoride (F), calcium (Ca), and phosphorus (P) in the enamel. The data were subjected to ANOVA (1-criterion), followed by the Student-Newman-Keuls test (p 0.001). The 1100F-TMP-MI Paste Plus group showed the highest concentration of Ca and P in the enamel (p < 0.001). CONCLUSION The application of 1100F-TMP-MI Paste Plus promoted a higher inhibitory effect against enamel demineralization. CLINICAL SIGNIFICANCE The combination of treatments with F, TMP, and MI Paste Plus® can be an effective alternative to improve the oral health of individuals, especially those with high activity of dental caries and at high risk for its development.
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Incorporation of chlorhexidine and nano-sized Sodium Trimetaphosphate into a glass-ionomer cement: Effect on mechanical and microbiological properties and inhibition of enamel demineralization
Journal of dentistry, 2019Co-Authors: Marjully Eduardo Rodrigues Da Silva, Denise Pedrini, Alberto Carlos Botazzo Delbem, Marcelle Danelon, José Antonio Santos Souza, Emerson R Camargo, Dinah Fressato Silva, Jesse Augusto Pereira, Cristiane DuqueAbstract:Abstract Objective To evaluate the antimicrobial/antibiofilm and mechanical properties, and the effect on enamel demineralization of a resin-modified GIC (RMGIC) containing CHX and nano-sized Sodium Trimetaphosphate (TMP). Methods RMGIC was associated with CHX (1.25 or 2.5%) and/or TMP (7 or 14%). Antimicrobial and antibiofilm activity were assessed using agar diffusion test and evaluation of biofilm metabolism, respectively. In addition, fluoride (F) and TMP releases as well as the diametral tensile (DTS) and compressive (CS) strength were determined. The percentage of mineral loss (%SH), integrated loss of subsurface hardness (ΔKHN) and enamel F concentrations were also evaluated. Results RMGICs containing CHX associated or not with TMP presented higher inhibition zones and effect on S. mutans biofilm. A reduction on CS was observed only for RMGIC + 2.5%CHX and on DTS for RMGIC + 2.5%CHX + 14%TMP. The highest F and TMP releases and lowest %SH and ΔKHN values were detected for RMGIC + 1.25%CHX + 14%TMP and RMGIC + 2.5%CHX + 14%TMP. Higher enamel F concentrations were observed for TMP groups. Conclusion 1.25%CHX and 14%TMP increased antimicrobial/antibiofilm action and the ability to prevent enamel demineralization, with minimal effect on the mechanical properties of RMGIC. Clinical significance RMGIC containing CHX and TMP is an alternative material for patients at high risk for dental caries and can be indicated for low-stress regions or provisional restorations.
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In situ effect of fluoride toothpaste supplemented with nano-sized Sodium Trimetaphosphate on enamel demineralization prevention and biofilm composition.
Archives of oral biology, 2018Co-Authors: Alberto Carlos Botazzo Delbem, Juliano Pelim Pessan, Emerson R Camargo, Gabriel Pereira Nunes, Francisco Nunes De Souza Neto, Marcelle DanelonAbstract:Abstract Objective To evaluate the effect of a fluoride toothpaste containing nano-sized Sodium Trimetaphosphate (TMPnano) on enamel demineralization in situ and composition of the biofilm. Design This crossover double-blind study consisted of four phases (seven days each) and 12 volunteers who wore oral appliances containing four enamel bovine blocks. The cariogenic challenge was performed by 30% sucrose solution (6x/day). The toothpaste treatments (3x/day) were as follows: no F/TMP/TMPnano (Placebo), 1100 ppm F (1100F), 1100F plus 3% micrometric or nano-sized TMP (1100F/TMP; 1100F/TMPnano). Percentage of surface hardness loss (%SH), and integrated loss of subsurface hardness (ΔKHN), as well as enamel calcium (Ca), phosphorus (P), and fluoride (F) were determined. Moreover, biofilm formed on the blocks were analyzed for F, Ca, P, and insoluble extracellular polysaccharide (EPS) concentrations. Data were analyzed using one-way ANOVA, repeated measures followed by Fisher LSD test (p Results 1100F/TMPnano promoted the lowest %SH and ΔKHN among all groups (p Conclusion 1100F/TMPnano promoted a greater protective effect against enamel demineralization and significantly affected the composition of biofilm formed in situ when compared to 1100F toothpaste.
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In vitro enamel remineralization capacity of composite resins containing Sodium Trimetaphosphate and fluoride
Clinical Oral Investigations, 2015Co-Authors: Adelisa Rodolfo Ferreira Tiveron, Kikue Takebayashi Sassaki, Alberto Carlos Botazzo Delbem, Gabriel Gaban, Denise PedriniAbstract:Objectives This study evaluated the in vitro enamel remineralization capacity of experimental composite resins containing Sodium Trimetaphosphate (TMP) combined or not with fluoride (F). Materials and methods Bovine enamel slabs were selected upon analysis of initial surface hardness (SH_1) and after induction of artificial carious lesions (SH_2). Experimental resins were as follows: resin C (control—no Sodium fluoride (NaF) or TMP), resin F (with 1.6 % NaF), resin TMP (with 14.1 % TMP), and resin TMP/F (with NaF and TMP). Resin samples were made and attached to enamel slabs ( n = 12 slabs per material). Those specimens (resin/enamel slab) were subjected to pH cycling to promote remineralization, and then final surface hardness (SH_3) was measured to calculate the percentage of surface hardness recovery (%SH). The integrated recovery of subsurface hardness (ΔKHN) and F concentration in enamel were also determined. Data was analyzed by ANOVA and Student–Newman–Keuls test ( p
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in vitro enamel remineralization capacity of composite resins containing Sodium Trimetaphosphate and fluoride
Clinical Oral Investigations, 2015Co-Authors: Adelisa Rodolfo Ferreira Tiveron, Kikue Takebayashi Sassaki, Alberto Carlos Botazzo Delbem, Gabriel Gaban, Denise PedriniAbstract:This study evaluated the in vitro enamel remineralization capacity of experimental composite resins containing Sodium Trimetaphosphate (TMP) combined or not with fluoride (F). Bovine enamel slabs were selected upon analysis of initial surface hardness (SH1) and after induction of artificial carious lesions (SH2). Experimental resins were as follows: resin C (control—no Sodium fluoride (NaF) or TMP), resin F (with 1.6 % NaF), resin TMP (with 14.1 % TMP), and resin TMP/F (with NaF and TMP). Resin samples were made and attached to enamel slabs (n = 12 slabs per material). Those specimens (resin/enamel slab) were subjected to pH cycling to promote remineralization, and then final surface hardness (SH3) was measured to calculate the percentage of surface hardness recovery (%SH). The integrated recovery of subsurface hardness (ΔKHN) and F concentration in enamel were also determined. Data was analyzed by ANOVA and Student–Newman–Keuls test (p < 0.05). Resins F and TMP/F showed similar SH3 values (p = 0.478) and %SH (p = 0.336) and differed significantly from the other resins (p < 0.001). Considering ΔKHN values, resin TMP/F presented the lowest area of lesion (p < 0.001). The presence of F on enamel was different among the fluoridated resins (p = 0.042), but higher than in the other resins (p < 0.001). The addition of TMP to a fluoridated composite resin enhanced its capacity for remineralization of enamel in vitro. The combination of two agents with action on enamel favored remineralization, suggesting that composite resins containing Sodium Trimetaphosphate and fluoride could be indicated for clinical procedures in situations with higher cariogenic challenges.
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In vitro enamel remineralization capacity of composite resins containing Sodium Trimetaphosphate and fluoride
Clinical oral investigations, 2015Co-Authors: Adelisa Rodolfo Ferreira Tiveron, Kikue Takebayashi Sassaki, Alberto Carlos Botazzo Delbem, Gabriel Gaban, Denise PedriniAbstract:This study evaluated the in vitro enamel remineralization capacity of experimental composite resins containing Sodium Trimetaphosphate (TMP) combined or not with fluoride (F). Bovine enamel slabs were selected upon analysis of initial surface hardness (SH1) and after induction of artificial carious lesions (SH2). Experimental resins were as follows: resin C (control—no Sodium fluoride (NaF) or TMP), resin F (with 1.6 % NaF), resin TMP (with 14.1 % TMP), and resin TMP/F (with NaF and TMP). Resin samples were made and attached to enamel slabs (n = 12 slabs per material). Those specimens (resin/enamel slab) were subjected to pH cycling to promote remineralization, and then final surface hardness (SH3) was measured to calculate the percentage of surface hardness recovery (%SH). The integrated recovery of subsurface hardness (ΔKHN) and F concentration in enamel were also determined. Data was analyzed by ANOVA and Student–Newman–Keuls test (p
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Effect of fluoride gels supplemented with Sodium Trimetaphosphate in reducing demineralization
Clinical Oral Investigations, 2014Co-Authors: Marcelle Danelon, Eliana Mitsue Takeshita, Ligia Carla Peixoto, Kikue Takebayashi Sassaki, Alberto Carlos Botazzo DelbemAbstract:Objectives The objective of this study was to evaluate the in vitro effect of low-fluoride (F) gels supplemented with Sodium Trimetaphosphate (TMP) on enamel demineralization. Materials and methods Bovine enamel blocks ( n = 160) were selected based on surface hardness (SH) and divided into eight treatment groups ( n = 20 per group): no F or TMP (placebo), 3 % TMP (3 %TMP), 5 % TMP (5 %TMP), 4,500 μg F/g (4,500), 4,500 μg F/g + 3 % TMP (4,500 3 %TMP), 4,500 μg F/g + 5 % TMP (4,500 5 %TMP), 9,000 μg F/g (9,000), and 12,300 μg F/g (acid gel). Blocks were subjected to demineralization/remineralization cycling for 5 days. Subsequently, surface hardness (SH_1) and integrated loss of subsurface hardness (ΔKHN) were assessed, and the concentrations of loosely bound (CaF_2-like) and firmly bound (FA-like) formed and retained F were determined. Results The 4,500 5 %TMP and acid gel groups showed similar results and had the lowest mineral loss (SH_1 and ∆KHN). The acid gel group had the highest concentration of CaF_2-like F, but the formation and retention of FA-like F was greater in the 4,500 5 %TMP group than in the acid gel group ( p
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effect of fluoride varnish supplemented with Sodium Trimetaphosphate on enamel erosion and abrasion
American Journal of Dentistry, 2013Co-Authors: M. M. Manarelli, Juliano Pelim Pessan, Kikue Takebayashi Sassaki, M J Moretto, Cleide Cristina Rodrigues Martinhon, Alberto Carlos Botazzo DelbemAbstract:PURPOSE: To assess in vitro the effect on enamel erosion (ERO) and erosion followed by abrasion (ERO+ABR) of varnishes with different fluoride concentrations, supplemented or not with Sodium Trimetaphosphate (TMP). METHODS: Bovine enamel blocks were randomly divided into six groups according to the type of varnish used: placebo (no F), NaF 5%, NaF 2.5%, NaF 2.5% plus TMP 3.5%, NaF 2.5% plus TMP 5%, NaF 2.5% plus TMP 10%. Varnishes were tested for ERO and ERO+ABR, separately for 3 and 5 days. ERO was done by immersion in Sprite Zero (5 minutes, 4x/day), while ERO+ABR was performed by brushing for 15 seconds after each erosive challenge. Enamel wear (microm) and cross-sectional hardness (AKHN) were assessed after the experimental periods. Data were analyzed by ANOVA, Tukey's test and Pearson's correlation coefficient (P 0.05). Greater wear was observed after 5 days of ERO and ERO+ABR when compared with 3 days (P< 0.05). Positive and significant correlations were found between enamel wear and AKHN. No dose-response relationship was found between TMP concentration and wear and hardness. It was concluded that fluoride varnishes supplemented with TMP had a higher protective effect against ERO and ERO+ABR, which was associated with a reduction in enamel softening.