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Alberto Carlos Botazzo Delbem - One of the best experts on this subject based on the ideXlab platform.
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protective effect of fluoride varnish containing Trimetaphosphate against dentin erosion and erosion abrasion an in vitro study
Caries Research, 2020Co-Authors: Marcelle Danelon, Juliano Pelim Pessan, M J Moretto, Cleide Cristina Rodrigues Martinhon, Kamila Miranda Prado, Jessica Paula Ramos, Nayara Goncalves Emerenciano, Alberto Carlos Botazzo DelbemAbstract:This in vitro study evaluated the protective effect of fluoride varnishes containing sodium Trimetaphosphate (TMP) against dentin erosion and abrasion. Specimens of coronal dentin were divided into: placebo, 2.5% NaF, 5% NaF, 2.5% NaF + 5% TMP, and 5% NaF + 5% TMP groups (n =24/group). After single application of the varnishes, the samples were immersed in citric acid (0.05 mol/L, pH = 3.2, 5 min) followed or not by brushing, and the dentin wear was assessed after 5 days. Varnishes containing fluoride + TMP led to the lowest wear. TMP varnishes showed a superior effect against dentin erosive wear.
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Activity of sodium Trimetaphosphate, associated or not with fluoride, on dual-species biofilms.
Biofouling, 2019Co-Authors: Thamires Priscila Cavazana, Juliano Pelim Pessan, Ty Hosida, Sampaio C, Douglas Roberto Monteiro, Alberto Carlos Botazzo DelbemAbstract:This study aimed to evaluate the effect of sodium Trimetaphosphate (TMP), either with fluoride (F) or without fluoride, on dual-species biofilms of Streptococcus mutans and Candida albicans. The 72...
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dentinal tubule obliteration using toothpastes containing sodium Trimetaphosphate microparticles or nanoparticles
Clinical Oral Investigations, 2018Co-Authors: Carla Oliveira Favretto, Alberto Carlos Botazzo Delbem, João Carlos Silos Moraes, Emerson R Camargo, Priscila Toninatto Alves De Toledo, Denise PedriniAbstract:This in vitro study evaluated the effect of microparticles (TMPmicro) or nanoparticles (TMPnano) TMP associated with fluoride (F) in toothpaste formulations on the obliteration of dentinal tubules (DT). The dentinal tubules of bovine dentin blocks were unobstructed by immersion in 37% phosphoric acid solution. Blocks (n = 20/group) underwent mechanical brushing (2×/day) during 7 days with toothpastes: placebo, 1100 ppm F, and 1100 with 3% TMPmicro or 3% TMPnano. After that, ten blocks of each group were immersed in citric acid (pH 3.2) for 1 min. The number, diameter and area of unobstructed DT, atomic % of chemical elements from precipitates, and the mineral concentration were quantified. Data were submitted to two-way ANOVA, followed by Student-Newman-Keuls test (P < 0.05). Toothpastes containing TMPmicro and TMPnano promoted greater obliteration of DT and greater mineral concentration compared to other groups (P < 0.05). Placebo and 1100 ppm F group presented similar obliteration but 1100 ppm F group promoted higher mineral concentration. Higher Ca/P ratios were observed in groups treated with TMP; the acid challenge reduced Ca/P ratio for all groups. The atomic % of C and N was significantly lower for TMP toothpastes before acid challenge, but increased afterwards. It was concluded that the addition of TMPmicro or TMPnano to F toothpastes produced greater obliteration of dentinal tubules and higher mineral concentration when compared with 1100 ppm F. By the addition of TMP, fluoride toothpastes may occlude the dentinal tubules, with potential to reduce dentin hypersensitivity.
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fluoride toothpastes containing micrometric or nano sized sodium Trimetaphosphate reduce enamel erosion in vitro
Acta Odontologica Scandinavica, 2018Co-Authors: Marcelle Danelon, Juliano Pelim Pessan, Emerson R Camargo, Vinicius Dos Santos, E K Chiba, Luhana Santos Gonzales Garcia, Alberto Carlos Botazzo DelbemAbstract:Objective: To evaluate the effect of fluoride toothpastes supplemented with micrometric or nano-sized sodium Trimetaphosphate (TMP or TMPnano, respectively) on enamel erosion in vitro, as well as t...
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toothpaste with nanosized Trimetaphosphate reduces enamel demineralization
JDR clinical and translational research, 2017Co-Authors: Juliano Pelim Pessan, José Antonio Santos Souza, Emerson R Camargo, Mdb Souza, Carolina Simonetti Lodi, F Souza N Neto, Alberto Carlos Botazzo DelbemAbstract:This double-blind crossover study assessed the effects of a low-fluoride (low-F) dentifrice containing nanosized sodium Trimetaphosphate (TMP) on enamel demineralization in situ. Nineteen subjects wore palatal appliances containing 4 blocks of bovine enamel and were randomly assigned to brush their teeth with placebo (without F/TMP), 250-ppm F (250F), 250F plus 0.05% nanosized TMP (250F-TMPnano), and 1,100-ppm F (1,100F) dentifrices during 7 d, under cariogenic challenge. Enamel surface hardness and cross-sectional hardness (ΔKHN [Knoop hardness number]), as well as F, calcium (Ca), and phosphorus (P) concentrations, were determined. Also, biofilm that formed on the blocks was analyzed for F, Ca, P, and insoluble extracellular polysaccharide concentrations. Data were submitted to analysis-of-variance models and Student-Newman-Keuls test ( P < 0.05). The 250F-TMPnano dentifrice promoted the lowest ΔKHN among all groups ( P < 0.001), while the percentage of surface hardness loss was similar to 1,100F. Also, similar F, Ca, and P concentrations in enamel were observed for 1,100F and 250F-TMPnano. In the biofilm, the highest F content was observed for 1,100F; Ca content was similar between 1,100F and 250F-TMPnano; and P content was similar among all groups. Similar extracellular polysaccharide values were observed for 250F-TMPnano and 1,100F ( P < 0.001), ionic activity of CaHPO40, CaF+, and HF0 ( P < 0.05) and degree of saturation of hydroxyapatite and CaF2 ( P < 0.05). It was concluded that the protective effect of 250F-TMPnano dentifrice was similar to a conventional dentifrice for most of the variables studied, having a more pronounced effect on the subsurface lesion when compared with the conventional toothpaste (1,100F). Knowledge Transfer Statement: Although toothpastes containing ≥1,000-ppm fluoride are more effective than low-fluoride formulations against dental caries, their early use can lead to side effects. This has prompted intensive research on alternatives to increase the anticaries effect of low-fluoride toothpastes. The present in situ study demonstrated that the addition of sodium Trimetaphosphate nanoparticles to toothpastes containing 250-ppm fluoride significantly enhances the protective effect of this formulation against enamel demineralization to levels comparable to a 1,100-ppm fluoride toothpaste in terms of most of the variables studied. Most important, this formulation promoted the lowest loss of subsurface hardness among all groups, suggesting that caries lesions would take longer to develop under clinical conditions when compared with a conventional (1,100-fluoride) toothpaste.
Scott D. Taylor - One of the best experts on this subject based on the ideXlab platform.
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synthesis of nucleoside 5 o tetraphosphates from activated Trimetaphosphate and nucleoside 5 o monophosphates
Current protocols in human genetics, 2018Co-Authors: Samy Mohamady, Scott D. TaylorAbstract:This article describes a straight-forward chemical method for the synthesis of nucleoside-5'-O-tetraphosphates, such as cytosine-, guanosine-, adenosine-, and uridine-5'-O-tetraphosphates, starting from the corresponding nucleoside monophosphates and Trimetaphosphate, a readily available and inexpensive starting material. The procedure involves reacting the tri(tetrabutylammonium) salt of Trimetaphosphate with mesitylenesulfonyl chloride and N-methylimidazole. The resulting activated cyclic Trimetaphosphate is reacted with the tetrabutylammonium salts of nucleoside monophosphates. After quenching the reaction with buffer and high-performance liquid chromatography purification, the desired nucleoside-5'-O-tetraphosphates were obtained in yields of 84% to 86%. © 2018 by John Wiley & Sons, Inc.
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one flask synthesis of 2 3 cyclic nucleoside monophosphates from unprotected nucleosides using activated cyclic Trimetaphosphate
Tetrahedron Letters, 2016Co-Authors: Samy Mohamady, Scott D. TaylorAbstract:The 2′,3′-cyclic nucleoside monophosphates (2′,3′-cNMPs) of adenosine, guanosine, cytidine, and uridine were prepared in good yield in a one pot reaction from unprotected ribonucleosides and activated Trimetaphosphate in the presence of N-methylimidazole, 4-nitroimidazole and MgCl2.
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synthesis of nucleoside triphosphates from 2 3 protected nucleosides using Trimetaphosphate
Organic Letters, 2016Co-Authors: Samy Mohamady, Scott D. TaylorAbstract:Chemists have been attempting to triphosphorylate nucleosides and other alcohols using Trimetaphosphate (TriMP) since the 1960s. However, this route appears to have been abandoned due to poor yields. The first practical syntheses of nucleoside triphosphates (NTPs) are reported using TriMP as the key reagent. This was achieved by reacting the tetrabutylammonium salt of TriMP with mesitylenesulfonyl chloride in the presence of DABCO in pyridine followed by the addition of an appropriately protected nucleoside and phthalimide. Quenching the reaction with aqueous buffer followed by hydrolysis of the OH protecting groups gave the NTPs in good yield.
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Synthesis of Nucleoside Triphosphates from 2′-3′-Protected Nucleosides Using Trimetaphosphate
2016Co-Authors: Samy Mohamady, Scott D. TaylorAbstract:Chemists have been attempting to triphosphorylate nucleosides and other alcohols using Trimetaphosphate (TriMP) since the 1960s. However, this route appears to have been abandoned due to poor yields. The first practical syntheses of nucleoside triphosphates (NTPs) are reported using TriMP as the key reagent. This was achieved by reacting the tetrabutylammonium salt of TriMP with mesitylenesulfonyl chloride in the presence of DABCO in pyridine followed by the addition of an appropriately protected nucleoside and phthalimide. Quenching the reaction with aqueous buffer followed by hydrolysis of the OH protecting groups gave the NTPs in good yield
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synthesis of nucleoside 5 tetraphosphates containing terminal fluorescent labels via activated cyclic Trimetaphosphate
Journal of Organic Chemistry, 2014Co-Authors: Samy Mohamady, Scott D. TaylorAbstract:2'-Deoxynucleotide 5'-tetraphosphates in which a fluorescent label is attached to the terminal phosphate are used as key reagents in high-throughput DNA sequencing techniques and in single nucleotide polymorphism typing assays. We demonstrate that this class of compounds can be prepared by reacting fluorophores such as 7-hydroxy-4-methylcoumarin, methylfluorescein, fluorescein and resorufin with an activated form of cyclic Trimetaphosphate to give intermediate 11. Reaction of 11 with 2'-deoxynucleoside 5'-monophosphates or a nucleoside 5'-monophosphate gave the target compounds in good yield.
Samy Mohamady - One of the best experts on this subject based on the ideXlab platform.
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synthesis of nucleoside 5 o tetraphosphates from activated Trimetaphosphate and nucleoside 5 o monophosphates
Current protocols in human genetics, 2018Co-Authors: Samy Mohamady, Scott D. TaylorAbstract:This article describes a straight-forward chemical method for the synthesis of nucleoside-5'-O-tetraphosphates, such as cytosine-, guanosine-, adenosine-, and uridine-5'-O-tetraphosphates, starting from the corresponding nucleoside monophosphates and Trimetaphosphate, a readily available and inexpensive starting material. The procedure involves reacting the tri(tetrabutylammonium) salt of Trimetaphosphate with mesitylenesulfonyl chloride and N-methylimidazole. The resulting activated cyclic Trimetaphosphate is reacted with the tetrabutylammonium salts of nucleoside monophosphates. After quenching the reaction with buffer and high-performance liquid chromatography purification, the desired nucleoside-5'-O-tetraphosphates were obtained in yields of 84% to 86%. © 2018 by John Wiley & Sons, Inc.
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one flask synthesis of 2 3 cyclic nucleoside monophosphates from unprotected nucleosides using activated cyclic Trimetaphosphate
Tetrahedron Letters, 2016Co-Authors: Samy Mohamady, Scott D. TaylorAbstract:The 2′,3′-cyclic nucleoside monophosphates (2′,3′-cNMPs) of adenosine, guanosine, cytidine, and uridine were prepared in good yield in a one pot reaction from unprotected ribonucleosides and activated Trimetaphosphate in the presence of N-methylimidazole, 4-nitroimidazole and MgCl2.
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synthesis of nucleoside triphosphates from 2 3 protected nucleosides using Trimetaphosphate
Organic Letters, 2016Co-Authors: Samy Mohamady, Scott D. TaylorAbstract:Chemists have been attempting to triphosphorylate nucleosides and other alcohols using Trimetaphosphate (TriMP) since the 1960s. However, this route appears to have been abandoned due to poor yields. The first practical syntheses of nucleoside triphosphates (NTPs) are reported using TriMP as the key reagent. This was achieved by reacting the tetrabutylammonium salt of TriMP with mesitylenesulfonyl chloride in the presence of DABCO in pyridine followed by the addition of an appropriately protected nucleoside and phthalimide. Quenching the reaction with aqueous buffer followed by hydrolysis of the OH protecting groups gave the NTPs in good yield.
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Synthesis of Nucleoside Triphosphates from 2′-3′-Protected Nucleosides Using Trimetaphosphate
2016Co-Authors: Samy Mohamady, Scott D. TaylorAbstract:Chemists have been attempting to triphosphorylate nucleosides and other alcohols using Trimetaphosphate (TriMP) since the 1960s. However, this route appears to have been abandoned due to poor yields. The first practical syntheses of nucleoside triphosphates (NTPs) are reported using TriMP as the key reagent. This was achieved by reacting the tetrabutylammonium salt of TriMP with mesitylenesulfonyl chloride in the presence of DABCO in pyridine followed by the addition of an appropriately protected nucleoside and phthalimide. Quenching the reaction with aqueous buffer followed by hydrolysis of the OH protecting groups gave the NTPs in good yield
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synthesis of nucleoside 5 tetraphosphates containing terminal fluorescent labels via activated cyclic Trimetaphosphate
Journal of Organic Chemistry, 2014Co-Authors: Samy Mohamady, Scott D. TaylorAbstract:2'-Deoxynucleotide 5'-tetraphosphates in which a fluorescent label is attached to the terminal phosphate are used as key reagents in high-throughput DNA sequencing techniques and in single nucleotide polymorphism typing assays. We demonstrate that this class of compounds can be prepared by reacting fluorophores such as 7-hydroxy-4-methylcoumarin, methylfluorescein, fluorescein and resorufin with an activated form of cyclic Trimetaphosphate to give intermediate 11. Reaction of 11 with 2'-deoxynucleoside 5'-monophosphates or a nucleoside 5'-monophosphate gave the target compounds in good yield.
Juliano Pelim Pessan - One of the best experts on this subject based on the ideXlab platform.
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protective effect of fluoride varnish containing Trimetaphosphate against dentin erosion and erosion abrasion an in vitro study
Caries Research, 2020Co-Authors: Marcelle Danelon, Juliano Pelim Pessan, M J Moretto, Cleide Cristina Rodrigues Martinhon, Kamila Miranda Prado, Jessica Paula Ramos, Nayara Goncalves Emerenciano, Alberto Carlos Botazzo DelbemAbstract:This in vitro study evaluated the protective effect of fluoride varnishes containing sodium Trimetaphosphate (TMP) against dentin erosion and abrasion. Specimens of coronal dentin were divided into: placebo, 2.5% NaF, 5% NaF, 2.5% NaF + 5% TMP, and 5% NaF + 5% TMP groups (n =24/group). After single application of the varnishes, the samples were immersed in citric acid (0.05 mol/L, pH = 3.2, 5 min) followed or not by brushing, and the dentin wear was assessed after 5 days. Varnishes containing fluoride + TMP led to the lowest wear. TMP varnishes showed a superior effect against dentin erosive wear.
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Activity of sodium Trimetaphosphate, associated or not with fluoride, on dual-species biofilms.
Biofouling, 2019Co-Authors: Thamires Priscila Cavazana, Juliano Pelim Pessan, Ty Hosida, Sampaio C, Douglas Roberto Monteiro, Alberto Carlos Botazzo DelbemAbstract:This study aimed to evaluate the effect of sodium Trimetaphosphate (TMP), either with fluoride (F) or without fluoride, on dual-species biofilms of Streptococcus mutans and Candida albicans. The 72...
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fluoride toothpastes containing micrometric or nano sized sodium Trimetaphosphate reduce enamel erosion in vitro
Acta Odontologica Scandinavica, 2018Co-Authors: Marcelle Danelon, Juliano Pelim Pessan, Emerson R Camargo, Vinicius Dos Santos, E K Chiba, Luhana Santos Gonzales Garcia, Alberto Carlos Botazzo DelbemAbstract:Objective: To evaluate the effect of fluoride toothpastes supplemented with micrometric or nano-sized sodium Trimetaphosphate (TMP or TMPnano, respectively) on enamel erosion in vitro, as well as t...
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toothpaste with nanosized Trimetaphosphate reduces enamel demineralization
JDR clinical and translational research, 2017Co-Authors: Juliano Pelim Pessan, José Antonio Santos Souza, Emerson R Camargo, Mdb Souza, Carolina Simonetti Lodi, F Souza N Neto, Alberto Carlos Botazzo DelbemAbstract:This double-blind crossover study assessed the effects of a low-fluoride (low-F) dentifrice containing nanosized sodium Trimetaphosphate (TMP) on enamel demineralization in situ. Nineteen subjects wore palatal appliances containing 4 blocks of bovine enamel and were randomly assigned to brush their teeth with placebo (without F/TMP), 250-ppm F (250F), 250F plus 0.05% nanosized TMP (250F-TMPnano), and 1,100-ppm F (1,100F) dentifrices during 7 d, under cariogenic challenge. Enamel surface hardness and cross-sectional hardness (ΔKHN [Knoop hardness number]), as well as F, calcium (Ca), and phosphorus (P) concentrations, were determined. Also, biofilm that formed on the blocks was analyzed for F, Ca, P, and insoluble extracellular polysaccharide concentrations. Data were submitted to analysis-of-variance models and Student-Newman-Keuls test ( P < 0.05). The 250F-TMPnano dentifrice promoted the lowest ΔKHN among all groups ( P < 0.001), while the percentage of surface hardness loss was similar to 1,100F. Also, similar F, Ca, and P concentrations in enamel were observed for 1,100F and 250F-TMPnano. In the biofilm, the highest F content was observed for 1,100F; Ca content was similar between 1,100F and 250F-TMPnano; and P content was similar among all groups. Similar extracellular polysaccharide values were observed for 250F-TMPnano and 1,100F ( P < 0.001), ionic activity of CaHPO40, CaF+, and HF0 ( P < 0.05) and degree of saturation of hydroxyapatite and CaF2 ( P < 0.05). It was concluded that the protective effect of 250F-TMPnano dentifrice was similar to a conventional dentifrice for most of the variables studied, having a more pronounced effect on the subsurface lesion when compared with the conventional toothpaste (1,100F). Knowledge Transfer Statement: Although toothpastes containing ≥1,000-ppm fluoride are more effective than low-fluoride formulations against dental caries, their early use can lead to side effects. This has prompted intensive research on alternatives to increase the anticaries effect of low-fluoride toothpastes. The present in situ study demonstrated that the addition of sodium Trimetaphosphate nanoparticles to toothpastes containing 250-ppm fluoride significantly enhances the protective effect of this formulation against enamel demineralization to levels comparable to a 1,100-ppm fluoride toothpaste in terms of most of the variables studied. Most important, this formulation promoted the lowest loss of subsurface hardness among all groups, suggesting that caries lesions would take longer to develop under clinical conditions when compared with a conventional (1,100-fluoride) toothpaste.
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fluoride varnishes containing sodium Trimetaphosphate reduce enamel demineralization in vitro
Acta Odontologica Scandinavica, 2017Co-Authors: M. M. Manarelli, Alberto Carlos Botazzo Delbem, Felipe Ricardo Nunes De Moraes, Robson Frederico Cunha, Liliana Carolina Baezquintero, Juliano Pelim PessanAbstract:AbstractObjective: This study evaluated the effects of fluoride varnishes containing sodium Trimetaphosphate (TMP) on bovine enamel demineralization in vitro.Material and methods: Enamel bovine discs were randomly assigned into six groups (n = 20/group): placebo, 2.5% NaF, 2.5% NaF/5% TMP, 5% NaF, 5% NaF/5% TMP, and a commercial formulation (Duraphat, 5% NaF). Varnishes were applied on all enamel discs and kept for 6 h. Loosely and firmly bound fluoride formed on/in enamel after treatment were analyzed in 10 discs from each group. The other 10 discs were subjected to a pH-cycling regimen for 7 days, and analyzed for surface (SH) and cross-sectional hardness (ΔKHN), as well as for loosely and firmly bound fluoride in/on enamel. Data were analyzed by analysis of variance (ANOVA) followed by Student–Newman–Keuls’ test (p < .05).Results: The lowest SH change and ΔKHN were observed for the 5%NaF/5%TMP varnish, which was significantly different from all the other groups. Both fluoridated varnishes containing TM...
Nidhi Bansal - One of the best experts on this subject based on the ideXlab platform.
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rheological behavior emulsifying properties and structural characterization of phosphorylated fish gelatin
Food Chemistry, 2018Co-Authors: Tao Huang, Zong Cai Tu, Xinchen Shangguan, Hui Wang, Nidhi BansalAbstract:Rheological, microstructural and emulsifying properties of fish gelatin phosphorylated using sodium Trimetaphosphate (STMP) were studied. Phosphorylation was carried out at 50 °C for 0, 0.5, 1 or 2 h. Rheological behaviors indicated that phosphorylation decreased gelation rate constant (k ) and apparent viscosity of gelatin solutions. Phosphorylation time was inversely proportional to tan δ; gelling and melting points of fish gelatin gels; however gel properties could be improved by short time of phosphorylation. Scanning electron microscopy and atomic force microscopy revealed that longer time of phosphorylation resulted in looser gel network with more aggregation. Longer phosphorylation time could stabilize fish gelatin emulsions, and endowed emulsions with smaller particle size and lower coefficient viscosity, but higher ζ-potential values. These results suggested that phosphorylation could be applied to obtain fish gelatin with varying functional properties suitable for numerous industrial applications.
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Rheological behavior, emulsifying properties and structural characterization of phosphorylated fish gelatin
Food Chemistry, 2018Co-Authors: Tao Huang, Zong Cai Tu, Xiao Mei Sha, Xinchen Shangguan, Hui Wang, Nidhi BansalAbstract:Rheological, microstructural and emulsifying properties of fish gelatin phosphorylated using sodium Trimetaphosphate (STMP) were studied. Phosphorylation was carried out at 50 °C for 0, 0.5, 1 or 2 h. Rheological behaviors indicated that phosphorylation decreased gelation rate constant (kgel) and apparent viscosity of gelatin solutions. Phosphorylation time was inversely proportional to tan δ; gelling and melting points of fish gelatin gels; however gel properties could be improved by short time of phosphorylation. Scanning electron microscopy and atomic force microscopy revealed that longer time of phosphorylation resulted in looser gel network with more aggregation. Longer phosphorylation time could stabilize fish gelatin emulsions, and endowed emulsions with smaller particle size and lower coefficient viscosity, but higher ζ-potential values. These results suggested that phosphorylation could be applied to obtain fish gelatin with varying functional properties suitable for numerous industrial applications.
