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P Morrondo - One of the best experts on this subject based on the ideXlab platform.
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comparison of oestrus ovis metabolic and Somatic Antigens for the immunodiagnosis of the zoonotic myasis oestrosis by immunoenzymatic probes
Immunological Investigations, 2005Co-Authors: R Sanchezandrade, J L Romero, J L Suarez, J Pedreira, P Diaz, M Arias, A Pazsilva, R Panadero, P Diezbanos, P MorrondoAbstract:Control of zoonosis implies reduction of infected animal hosts, and the first measure consists of a suitable and accurate detection test. An experimental study for determining the most appropriate antigen (metabolic or Somatic) to be used in the detection of the oestrosis (Oestrus ovis) zoonotic myasis by means of immunoenzymatic probes was carried out. A flock of 23 uninfected goats was maintained under field conditions to allow their infection in Sassari (Sardinia, Italy). Caprine were bled monthly and serum samples processed by means of an iELISA. After comparing these results to the chronobiology of O. ovis, we proved that the IgG humoral response against the metabolic Antigens increased only during the period of real risk of infestation (when adults fly, from May to September), whereas the absorbances against the Somatic products were positive from the beginning of the study (in January, prior to infection). We concluded that the excretory/secretory products are most useful andsuitable for the immuno...
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comparison of oestrus ovis metabolic and Somatic Antigens for the immunodiagnosis of the zoonotic myasis oestrosis by immunoenzymatic probes
Immunological Investigations, 2005Co-Authors: R Sanchezandrade, J L Romero, J L Suarez, J Pedreira, P Diaz, M Arias, A Pazsilva, R Panadero, P Diezbanos, P MorrondoAbstract:Control of zoonosis implies reduction of infected animal hosts, and the first measure consists of a suitable and accurate detection test. An experimental study for determining the most appropriate antigen (metabolic or Somatic) to be used in the detection of the oestrosis (Oestrus ovis) zoonotic myasis by means of immunoenzymatic probes was carried out. A flock of 23 uninfected goats was maintained under field conditions to allow their infection in Sassari (Sardinia, Italy). Caprine were bled monthly and serum samples processed by means of an iELISA. After comparing these results to the chronobiology of O. ovis, we proved that the IgG humoral response against the metabolic Antigens increased only during the period of real risk of infestation (when adults fly, from May to September), whereas the absorbances against the Somatic products were positive from the beginning of the study (in January, prior to infection). We concluded that the excretory/secretory products are most useful and suitable for the immunodiagnosis of oestrosis in goats, because a direct relation between the development of O. ovis and the IgG humoral response is possible, allowing a more accurate diagnostic.
J B W J Cornelissen - One of the best experts on this subject based on the ideXlab platform.
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comparison of three enzyme immunoassays for diagnosis of dictyocaulus viviparus infection
Veterinary Parasitology, 1993Co-Authors: W A De Leeuw, J B W J CornelissenAbstract:Three enzyme-linked immunosorbent assays (ELISA) that detect antibodies against Dictyocaulus viviparus in cattle were compared for sensitivity, specificity and seroconversion after primary infection. These assays were (i) an indirect ELISA with crude Somatic Antigens from adult D. viviparus (ca-ELISA), (ii) an indirect ELISA wash purified Antigens (sa-ELISA) isolated from Somatic Antigens of adult D. viviparus and (iii) a competition ELISA with antigen purified with anion chromatography in combination with monoclonal antibodies against D. viviparus. Sera from helminth-naive calves and sera from calves monospecifically infected with Ostertagia ostertagi, Cooperia oncophora, Nematodirus helvetianus, Ascaris suum or Fasciola hepatica were used to determine the specificity of the assays. Sera from calves and milk cows experimentally or naturally infected with D. viviparus, and from vaccinated calves, were used to test the sensitivity of the assays and to determine when the ELISAs detected seroconversion. The specificity of the competition and the sa-ELISA was 97%, whereas the specificity of the ca-ELISA was 67%. The sensitivities of the sa-ELISA, the competition ELISA and the ca-ELISA were 97, 73 and 99%, respectively. All three assays detected seroconversion between 4 and 6 weeks after primary infection. None of the assays detected seroconversion in calves receiving lungworm vaccination. We conclude that of these three tests, the sa-ELISA can be used most beneficially to diagnose lungworm disease.
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identification and isolation of a specific antigen with diagnostic potential from dictyocaulus viviparus
Veterinary Parasitology, 1991Co-Authors: W A De Leeuw, J B W J CornelissenAbstract:The purpose of the investigation was to isolate and identify a specific antigen of Dictyocaulus viviparus that can be used to diagnose lungworm infections in cattle. Somatic, excretion and secretion Antigens of adult D. viviparus and Somatic Antigens of L3 larvae were examined in an indirect enzymelinked immunosorbent assay (ELISA) to determine whether they cross-reacted with sera collected from calves with mono-infections of Fasciola hepatica, Ostertagia ostertagi, Ascaris suum, or Cooperia oncophora. Serum samples containing antibodies directed against F. hepatica, A. suum, and O. ostertagi cross-reacted with Somatic Antigens of adult D. viviparus; these sera cross-reacted less with excretion and secretion Antigens. When Somatic Antigens of adult D. viviparus were analysed in a Western blot, 1 17-kDa protein that did not react with the heterologous sera was detected. This protein was isolated by ultrafiltration and anion chromatograohy. Sera collected from calves infected with D. viviparus was tested in indirect ELISAs with either Somatic Antigens of adult D. viviparus or with a low molecular antigen fraction of this preparation containing the 17-kDa protein. The extinction values that were measured in both assays correlated well. We conclude that the 17-kDa protein isolated from Somatic Antigens of adult D. viviparus may be useful in developing an improved immunoassay to diagnose lungworm infections in cattle.
K. Aizawa - One of the best experts on this subject based on the ideXlab platform.
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serotyping of bacillus thuringiensis environmental isolates by extracellular heat stable Somatic Antigens
Canadian Journal of Microbiology, 1992Co-Authors: Michio Ohba, K. Ueda, K. AizawaAbstract:A total of 525 Bacillus thuringiensis environmental isolates, belonging to the five flagellar (H) serovars (alesti, sotto, kenyae, aizawai, and morrisoni), were serotyped by extracellular heat-stable Somatic Antigens (HSSAs). The isolates belonging to a given H serovar were assigned to a single HSSA serogroup at a high frequency, 87-100%. This indicates that the extent of HSSA variation within a single H serovar is small in the field populations of these B. thuringiensis serovars.
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Serogrouping of Bacillus thuringiensis by Extracellular Heat-Stable Somatic Antigens
Systematic and Applied Microbiology, 1991Co-Authors: K. Ueda, Michio Ohba, K. AizawaAbstract:Summary Serogrouping of Bacillus thuringiensis strains by extracellular heat-stable Somatic Antigens (HSSAs) was achieved on the basis of the formation of colony-surrounding immunoprecipitin halos on antiserum-agar plates. The type strains of 24 flagellar (H) antigenic serovars of B. thuringiensis were assigned to 16 extracellular HSSA serogroups. The results indicate a lack of correlation between serogroupings of B. thuringiensis based on extracellular HSSAs and H Antigens.
R Sanchezandrade - One of the best experts on this subject based on the ideXlab platform.
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comparison of oestrus ovis metabolic and Somatic Antigens for the immunodiagnosis of the zoonotic myasis oestrosis by immunoenzymatic probes
Immunological Investigations, 2005Co-Authors: R Sanchezandrade, J L Romero, J L Suarez, J Pedreira, P Diaz, M Arias, A Pazsilva, R Panadero, P Diezbanos, P MorrondoAbstract:Control of zoonosis implies reduction of infected animal hosts, and the first measure consists of a suitable and accurate detection test. An experimental study for determining the most appropriate antigen (metabolic or Somatic) to be used in the detection of the oestrosis (Oestrus ovis) zoonotic myasis by means of immunoenzymatic probes was carried out. A flock of 23 uninfected goats was maintained under field conditions to allow their infection in Sassari (Sardinia, Italy). Caprine were bled monthly and serum samples processed by means of an iELISA. After comparing these results to the chronobiology of O. ovis, we proved that the IgG humoral response against the metabolic Antigens increased only during the period of real risk of infestation (when adults fly, from May to September), whereas the absorbances against the Somatic products were positive from the beginning of the study (in January, prior to infection). We concluded that the excretory/secretory products are most useful andsuitable for the immuno...
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comparison of oestrus ovis metabolic and Somatic Antigens for the immunodiagnosis of the zoonotic myasis oestrosis by immunoenzymatic probes
Immunological Investigations, 2005Co-Authors: R Sanchezandrade, J L Romero, J L Suarez, J Pedreira, P Diaz, M Arias, A Pazsilva, R Panadero, P Diezbanos, P MorrondoAbstract:Control of zoonosis implies reduction of infected animal hosts, and the first measure consists of a suitable and accurate detection test. An experimental study for determining the most appropriate antigen (metabolic or Somatic) to be used in the detection of the oestrosis (Oestrus ovis) zoonotic myasis by means of immunoenzymatic probes was carried out. A flock of 23 uninfected goats was maintained under field conditions to allow their infection in Sassari (Sardinia, Italy). Caprine were bled monthly and serum samples processed by means of an iELISA. After comparing these results to the chronobiology of O. ovis, we proved that the IgG humoral response against the metabolic Antigens increased only during the period of real risk of infestation (when adults fly, from May to September), whereas the absorbances against the Somatic products were positive from the beginning of the study (in January, prior to infection). We concluded that the excretory/secretory products are most useful and suitable for the immunodiagnosis of oestrosis in goats, because a direct relation between the development of O. ovis and the IgG humoral response is possible, allowing a more accurate diagnostic.
W A De Leeuw - One of the best experts on this subject based on the ideXlab platform.
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comparison of three enzyme immunoassays for diagnosis of dictyocaulus viviparus infection
Veterinary Parasitology, 1993Co-Authors: W A De Leeuw, J B W J CornelissenAbstract:Three enzyme-linked immunosorbent assays (ELISA) that detect antibodies against Dictyocaulus viviparus in cattle were compared for sensitivity, specificity and seroconversion after primary infection. These assays were (i) an indirect ELISA with crude Somatic Antigens from adult D. viviparus (ca-ELISA), (ii) an indirect ELISA wash purified Antigens (sa-ELISA) isolated from Somatic Antigens of adult D. viviparus and (iii) a competition ELISA with antigen purified with anion chromatography in combination with monoclonal antibodies against D. viviparus. Sera from helminth-naive calves and sera from calves monospecifically infected with Ostertagia ostertagi, Cooperia oncophora, Nematodirus helvetianus, Ascaris suum or Fasciola hepatica were used to determine the specificity of the assays. Sera from calves and milk cows experimentally or naturally infected with D. viviparus, and from vaccinated calves, were used to test the sensitivity of the assays and to determine when the ELISAs detected seroconversion. The specificity of the competition and the sa-ELISA was 97%, whereas the specificity of the ca-ELISA was 67%. The sensitivities of the sa-ELISA, the competition ELISA and the ca-ELISA were 97, 73 and 99%, respectively. All three assays detected seroconversion between 4 and 6 weeks after primary infection. None of the assays detected seroconversion in calves receiving lungworm vaccination. We conclude that of these three tests, the sa-ELISA can be used most beneficially to diagnose lungworm disease.
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identification and isolation of a specific antigen with diagnostic potential from dictyocaulus viviparus
Veterinary Parasitology, 1991Co-Authors: W A De Leeuw, J B W J CornelissenAbstract:The purpose of the investigation was to isolate and identify a specific antigen of Dictyocaulus viviparus that can be used to diagnose lungworm infections in cattle. Somatic, excretion and secretion Antigens of adult D. viviparus and Somatic Antigens of L3 larvae were examined in an indirect enzymelinked immunosorbent assay (ELISA) to determine whether they cross-reacted with sera collected from calves with mono-infections of Fasciola hepatica, Ostertagia ostertagi, Ascaris suum, or Cooperia oncophora. Serum samples containing antibodies directed against F. hepatica, A. suum, and O. ostertagi cross-reacted with Somatic Antigens of adult D. viviparus; these sera cross-reacted less with excretion and secretion Antigens. When Somatic Antigens of adult D. viviparus were analysed in a Western blot, 1 17-kDa protein that did not react with the heterologous sera was detected. This protein was isolated by ultrafiltration and anion chromatograohy. Sera collected from calves infected with D. viviparus was tested in indirect ELISAs with either Somatic Antigens of adult D. viviparus or with a low molecular antigen fraction of this preparation containing the 17-kDa protein. The extinction values that were measured in both assays correlated well. We conclude that the 17-kDa protein isolated from Somatic Antigens of adult D. viviparus may be useful in developing an improved immunoassay to diagnose lungworm infections in cattle.
