The Experts below are selected from a list of 22716 Experts worldwide ranked by ideXlab platform
Xun Liao - One of the best experts on this subject based on the ideXlab platform.
-
rapid magnetic solid phase extraction for the selective determination of isoflavones in soymilk using baicalin functionalized magnetic nanoparticles
Journal of Agricultural and Food Chemistry, 2013Co-Authors: Linsen Qing, Ying Xue, Yiming Liu, Jian Liang, Jing Xie, Xun LiaoAbstract:Most protocols of sample preparation for isoflavone determination in soymilk and other liquid Soybean Products involves tedious freeze-drying and time-consuming extraction procedures. We report a facile and rapid magnetic solid-phase extraction (MSPE) of isoflavones from soymilk for subsequent high-performance liquid chromatography electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) analysis. The extraction was based on the selective binding of isoflavones to baicalin-functionalized core-shell magnetic nanoparticles (BMNPs). The proposed MSPE-HPLC-MS/MS analytical method had a linear calibration curve in the concentration range from 0.3 to 80 mg/L isoflavones. With the use of calycosin, an isomer of one of the isoflavones targeted as an internal standard, interday (5 days) precisions of the slope and intercept of the calibration curves were found to be in the range between 2.5% and 3.6% (RSD, n = 5). Six isoflavones, that is, daidzein, glycitein, genistein, daidzin, glycitin, and genistin were detected in commercial soymilk samples and quantified by the proposed analytical method. The results indicated that the method was useful for fast determination of isoflavones in soymilk and other liquid Soybean Products.
-
rapid magnetic solid phase extraction for the selective determination of isoflavones in soymilk using baicalin functionalized magnetic nanoparticles
Journal of Agricultural and Food Chemistry, 2013Co-Authors: Linsen Qing, Ying Xue, Yiming Liu, Jian Liang, Jing Xie, Xun LiaoAbstract:Most protocols of sample preparation for isoflavone determination in soymilk and other liquid Soybean Products involves tedious freeze-drying and time-consuming extraction procedures. We report a facile and rapid magnetic solid-phase extraction (MSPE) of isoflavones from soymilk for subsequent high-performance liquid chromatography electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) analysis. The extraction was based on the selective binding of isoflavones to baicalin-functionalized core–shell magnetic nanoparticles (BMNPs). The proposed MSPE-HPLC-MS/MS analytical method had a linear calibration curve in the concentration range from 0.3 to 80 mg/L isoflavones. With the use of calycosin, an isomer of one of the isoflavones targeted as an internal standard, interday (5 days) precisions of the slope and intercept of the calibration curves were found to be in the range between 2.5% and 3.6% (RSD, n = 5). Six isoflavones, that is, daidzein, glycitein, genistein, daidzin, glycitin, and genistin we...
Peter K Pauls - One of the best experts on this subject based on the ideXlab platform.
-
a versatile Soybean recombinant inbred line population segregating for low linolenic acid and lipoxygenase nulls molecular characterization and utility for soymilk and bioproduct production
2011Co-Authors: Yarmilla Reinprecht, Shunyan Luklabey, Peter K PaulsAbstract:The chapter aims to: (a) review the development of a Soybean recombinant inbred line (RIL) population obtained from the RG10 x OX948 cross that is segregating for low linolenic acid (LLA) and lipoxygenase nulls (3lx), (b) present the molecular characterization of the LLA and 3lx traits and (c) discuss the applicability of the population for soymilk and biocomposite production. The chapter is organized as follows. Section 2 describes development and evaluation of the RG10 x OX948 RIL population, inheritance of LLA and 3lx traits and the development of novel low linolenic acid, lipoxygenase free (LLA.3lx) germplasm. Section 3 describes the molecular characterization of the LLA and 3lx traits derived from RG10 and OX948, respectively. The versatility of the RIL population for different applications, including soymilk and bioproduct production are presented in Section 4, which illustrates that there is potential for utilizing the whole Soybean plant. The conclusion summarizes the results and discusses possible future uses of the RG10 x OX948 RIL population. Problem Oxidation of linolenic acid (LA) is catalyzed by lipoxygenase (LX; linoleate: oxygen oxidoreductase; EC 1.13.11.12) and is associated with off-flavours of Soybean Products. The hydroperoxides that are produced and their breakdown Products impart undesirable grassy-beany and bitter flavours on Soybean Products (Rackis et al., 1979). Most Soybean cultivars contain 70 to 90 g kg-1 LA and approximately 20 g kg-1 of the total seed protein is LX. A possible solution to Soybean stability problems is to genetically eliminate seed LX and reduce the content of LA. A number of Soybean cultivars with low LA content (LLA; < 40 g kg-1 LA) or seed LX nulls (Hammond et al., 1972; Hildebrand & Hymowitz, 1982; Wilcox, 1985; White, 2000) have been developed. It was shown that oil extracted from LLA lines is more stable than oil extracted from conventional Soybean, needs less hydrogenation and consequently, contains less unhealthy trans fatty acids (Mounts et al., 1988), which may reduce the risk of heart disease (Hayakawa et al., 2000). Similarly, protein Products from lx3 Soybean have more favourable flavour profiles (King et al., 1998). It has been suggested that a combination of seed LX nulls with LLA content might lead to even
-
seed and agronomic qtl in low linolenic acid lipoxygenase free Soybean glycine max l merrill germplasm
Genome, 2006Co-Authors: Yarmilla Reinprecht, Vaino Poysa, Istvan Rajcan, Gary R Ablett, Peter K PaulsAbstract:Linolenic acid and seed lipoxygenases are associated with off flavours in Soybean Products. F5 recombinant inbred lines (RILs) from a cross between a low linolenic acid line (RG10) and a seed lipoxygenase-free line (OX948) were genotyped for simple sequence repeats (SSR), random amplified polymorphic DNA (RAPD), sequence-tagged sites (STS), and cleaved amplified polymorphic sequence (CAPS) markers and evaluated for seed and agronomic traits at 3 Ontario locations in 2 years. One hundred twenty markers covering 1247.5 cM were mapped to 18 linkage groups (LGs) in the Soybean composite genetic map. Seed lipoxygenases L-1 and L-2 mapped as single major genes to the same location on LG G13-F. L-3 mapped to LG G11-E. This is the first report of a map position for L-3. A major quantitative trait locus (QTL) associated with reduced linolenic acid content was identified on LG G3-B2. QTLs for 12 additional seed and agronomic traits were detected. Linolenic acid content, linoleic acid content, yield, seed mass, prot...
-
seed and agronomic qtl in low linolenic acid lipoxygenase free Soybean glycine max l merrill germplasm
Genome, 2006Co-Authors: Yarmilla Reinprecht, Vaino Poysa, Istvan Rajcan, Gary R Ablett, Peter K PaulsAbstract:Linolenic acid and seed lipoxygenases are associated with off flavours in Soybean Products. F5 recombinant inbred lines (RILs) from a cross between a low linolenic acid line (RG10) and a seed lipox...
Paola Vitaglione - One of the best experts on this subject based on the ideXlab platform.
-
the quantification of free amadori compounds and amino acids allows to model the bound maillard reaction Products formation in Soybean Products
Food Chemistry, 2018Co-Authors: Antonio Dario Troise, Vincenzo Fogliano, Markus Wiltafsky, Paola VitaglioneAbstract:The quantification of protein bound Maillard reaction Products (MRPs) is still a challenge in food chemistry. Protein hydrolysis is the bottleneck step: it is time consuming and the protein degradation is not always complete. In this study, the quantitation of free amino acids and Amadori Products (APs) was compared to the percentage of blocked lysine by using chemometric tools. Eighty thermally treated Soybean samples were analyzed by mass spectrometry to measure the concentration of free amino acids, free APs and the protein-bound markers of the Maillard reaction (furosine, Ne-(carboxymethyl)-l-lysine, Ne-(carboxyethyl)-l-lysine, total lysine). Results demonstrated that Discriminant Analysis (DA) and Correlated Component Regression (CCR) correctly estimated the percent of blocked lysine in a validation and prediction set. These findings indicate that the measure of free markers reflects the extent of protein damage in Soybean samples and it suggests the possibility to obtain rapid information on the quality of the industrial processes.
Yiming Liu - One of the best experts on this subject based on the ideXlab platform.
-
rapid magnetic solid phase extraction for the selective determination of isoflavones in soymilk using baicalin functionalized magnetic nanoparticles
Journal of Agricultural and Food Chemistry, 2013Co-Authors: Linsen Qing, Ying Xue, Yiming Liu, Jian Liang, Jing Xie, Xun LiaoAbstract:Most protocols of sample preparation for isoflavone determination in soymilk and other liquid Soybean Products involves tedious freeze-drying and time-consuming extraction procedures. We report a facile and rapid magnetic solid-phase extraction (MSPE) of isoflavones from soymilk for subsequent high-performance liquid chromatography electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) analysis. The extraction was based on the selective binding of isoflavones to baicalin-functionalized core-shell magnetic nanoparticles (BMNPs). The proposed MSPE-HPLC-MS/MS analytical method had a linear calibration curve in the concentration range from 0.3 to 80 mg/L isoflavones. With the use of calycosin, an isomer of one of the isoflavones targeted as an internal standard, interday (5 days) precisions of the slope and intercept of the calibration curves were found to be in the range between 2.5% and 3.6% (RSD, n = 5). Six isoflavones, that is, daidzein, glycitein, genistein, daidzin, glycitin, and genistin were detected in commercial soymilk samples and quantified by the proposed analytical method. The results indicated that the method was useful for fast determination of isoflavones in soymilk and other liquid Soybean Products.
-
rapid magnetic solid phase extraction for the selective determination of isoflavones in soymilk using baicalin functionalized magnetic nanoparticles
Journal of Agricultural and Food Chemistry, 2013Co-Authors: Linsen Qing, Ying Xue, Yiming Liu, Jian Liang, Jing Xie, Xun LiaoAbstract:Most protocols of sample preparation for isoflavone determination in soymilk and other liquid Soybean Products involves tedious freeze-drying and time-consuming extraction procedures. We report a facile and rapid magnetic solid-phase extraction (MSPE) of isoflavones from soymilk for subsequent high-performance liquid chromatography electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) analysis. The extraction was based on the selective binding of isoflavones to baicalin-functionalized core–shell magnetic nanoparticles (BMNPs). The proposed MSPE-HPLC-MS/MS analytical method had a linear calibration curve in the concentration range from 0.3 to 80 mg/L isoflavones. With the use of calycosin, an isomer of one of the isoflavones targeted as an internal standard, interday (5 days) precisions of the slope and intercept of the calibration curves were found to be in the range between 2.5% and 3.6% (RSD, n = 5). Six isoflavones, that is, daidzein, glycitein, genistein, daidzin, glycitin, and genistin we...
Linsen Qing - One of the best experts on this subject based on the ideXlab platform.
-
rapid magnetic solid phase extraction for the selective determination of isoflavones in soymilk using baicalin functionalized magnetic nanoparticles
Journal of Agricultural and Food Chemistry, 2013Co-Authors: Linsen Qing, Ying Xue, Yiming Liu, Jian Liang, Jing Xie, Xun LiaoAbstract:Most protocols of sample preparation for isoflavone determination in soymilk and other liquid Soybean Products involves tedious freeze-drying and time-consuming extraction procedures. We report a facile and rapid magnetic solid-phase extraction (MSPE) of isoflavones from soymilk for subsequent high-performance liquid chromatography electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) analysis. The extraction was based on the selective binding of isoflavones to baicalin-functionalized core-shell magnetic nanoparticles (BMNPs). The proposed MSPE-HPLC-MS/MS analytical method had a linear calibration curve in the concentration range from 0.3 to 80 mg/L isoflavones. With the use of calycosin, an isomer of one of the isoflavones targeted as an internal standard, interday (5 days) precisions of the slope and intercept of the calibration curves were found to be in the range between 2.5% and 3.6% (RSD, n = 5). Six isoflavones, that is, daidzein, glycitein, genistein, daidzin, glycitin, and genistin were detected in commercial soymilk samples and quantified by the proposed analytical method. The results indicated that the method was useful for fast determination of isoflavones in soymilk and other liquid Soybean Products.
-
rapid magnetic solid phase extraction for the selective determination of isoflavones in soymilk using baicalin functionalized magnetic nanoparticles
Journal of Agricultural and Food Chemistry, 2013Co-Authors: Linsen Qing, Ying Xue, Yiming Liu, Jian Liang, Jing Xie, Xun LiaoAbstract:Most protocols of sample preparation for isoflavone determination in soymilk and other liquid Soybean Products involves tedious freeze-drying and time-consuming extraction procedures. We report a facile and rapid magnetic solid-phase extraction (MSPE) of isoflavones from soymilk for subsequent high-performance liquid chromatography electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) analysis. The extraction was based on the selective binding of isoflavones to baicalin-functionalized core–shell magnetic nanoparticles (BMNPs). The proposed MSPE-HPLC-MS/MS analytical method had a linear calibration curve in the concentration range from 0.3 to 80 mg/L isoflavones. With the use of calycosin, an isomer of one of the isoflavones targeted as an internal standard, interday (5 days) precisions of the slope and intercept of the calibration curves were found to be in the range between 2.5% and 3.6% (RSD, n = 5). Six isoflavones, that is, daidzein, glycitein, genistein, daidzin, glycitin, and genistin we...