The Experts below are selected from a list of 288 Experts worldwide ranked by ideXlab platform
Zhong Quan Wang - One of the best experts on this subject based on the ideXlab platform.
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Large-scale survey of a neglected agent of sparganosis Spirometra erinaceieuropaei (Cestoda: Diphyllobothriidae) in wild frogs in China.
PLOS Neglected Tropical Diseases, 2020Co-Authors: Xi Zhang, Peng Jiang, Xiu Hong, Shu Chuan Zhao, Zhong Quan WangAbstract:Background In China, frogs play an understudied role in the spread of human sparganosis (caused by the larval form of Spirometra). However, our knowledge about the prevalence of Sparganum infection in frogs remains fragmented, and the taxonomic identification of the parasite is still controversial. Methodology/Principal findings The prevalence of Sparganum infection in wild frogs was surveyed at 145 geographical locations from 28 of the 34 provinces/autonomous regions/municipalities in China for six years. The collected Sparganum isolates from the different locations were subjected to molecular identification by a multiplex PCR assay and then were analysed with clustering analysis. In the survey, Sparganum infection was found in 8 out of 13 of the collected frog species, and the most frequently infected species was Pelophylax nigromaculatus (the infection rate was up to 14.07%). Infected frogs were found in 80 of the 145 surveyed locations. The Sparganum infection rates in the wild frogs in several regions of China were still high (above 10%), especially in South and Southwest China. A total of 72 spargana were selected for molecular identification, and the clustering analysis showed that sequences from the Chinese isolates were very similar to those identified as from Spirometra erinaceieuropaei. However, the taxonomy of the genus remains confused and further analysis is required. Conclusions Eating wild frogs is associated with considerable health risks in China. Several traditional Chinese folk remedies may increase the risk of infection. The Sparganum isolates in China are most likely from S. erinaceieuropaei, but new studies, especially comprehensive morphological analyses, are needed in the future.
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development of est derived microsatellite markers to investigate the population structure of Sparganum the causative agent of zoonotic sparganosis
Parasitology, 2019Co-Authors: Xi Zhang, Peng Jiang, Xiu Hong, Jiang Yang Duan, Zi Yang Hong, Zhong Quan WangAbstract:: The plerocercoid (Sparganum) of Spirometra erinaceieuropaei is the main aetiological agent of human sparganosis. To improve the current knowledge on S. erinaceieuropaei evolution, we performed multi-locus microsatellite typing of Sparganum isolates from China for the first time. All available expressed sequence tag (EST) sequences for the Spirometra were downloaded from the GenBank. The identification and localization of microsatellites in ESTs was accomplished by MISA. Based on the selected microsatellites, the genetic structure of 64 Sparganum isolates collected from 11 geographical locations in southwest China were investigated through principal component analysis, STRUCTURE analysis and neighbour-joining clustering. A total of 522 non-redundant ESTs containing 915 simple sequence repeats were identified from 12 481 ESTs screened. Five primer pairs were finally selected. Using these loci, a total of 12 alleles were detected in 64 Sparganum isolates. Little variability was observed within each of geographical population, especially among isolates derived from Kunming of Yunnan (YN-KM) province. Both STRUCTURE analysis and the clustering analysis supported that two genotypes existed among the Sparganum isolates from southwest China. In conclusion, five microsatellite markers were successfully developed, and Sparganum population was observed to harbour low genetic variation, further investigation with deeper sampling was needed to elucidate the population structure.
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The phylogenetic diversity of Spirometra erinaceieuropaei isolates from southwest China revealed by multi genes.
Acta Tropica, 2016Co-Authors: Xi Zhang, Peng Jiang, Han Wang, Yun Lu Zhang, Zhong Quan WangAbstract:The larval plerocercoid of Spirometra erinaceieuropaei can parasitize humans, causing a serious food borne parasitic zoonosis known as sparganosis. Sparganosis have increased in China in recent years. In this study, the prevalence of Sparganum infection in wild frogs in 9 geographical areas in southwest China was firstly investigated. Of 276 caught frogs, 55 frogs were found to be infected with Sparganum. Then, the population genetic structure of these Sparganum isolates was explored based on four molecular markers (cytb, cox1, rrnS and 28S rDNA D1). Highly genetic diversity and the genetic differentiation among Sparganum isolates from different sites were revealed in the DNA polymorphism analyses. Both the phylogenetic inference and the analysis of the median-joining network supported two clades in the southwest S. erinaceieuropaei population. However, none demographic population expansion of the southwest S. erinaceieuropaei population was observed in the neutrality test, mismatch distribution analysis and Bayesian skyline plot analysis. Finally, the phylogenetic diversity of S. erinaceieuropaei from eastern, central, southern and southwest China was analyzed, the result suggested that Chinese S. erinaceieuropaei population should be divided into two groups (Group I and Group II), and they started to divergence in the middle Pliocene.
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Analysis of structure, function and epitopes of Spirometra erinaceieuropaei casein kinase I.
Tropical Biomedicine, 2015Co-Authors: Zhong Quan Wang, Zi Fang Zhang, Peng Jiang, Xi Zhang, G Y ZhangAbstract:: Spirometra erinaceieuropaei casein kinase I (SeCKI) was analyzed using bioinformatical methods to predict its structure and function based on the deduced amino acid sequence from full length cDNA sequence of SeCKI gene with online sites and software programs. The longest open reading frame contains 448 amino acids, 50 kDa and theoretical pI of 4.73, with a complete tubulin domain, a SMART tubulin_C domain and a low complexity region. SeCKI has no signal sequence and no transmembrane domain, but is predicted to be located extracellularly. The secondary structure of SeCKI contains 12 α-helixes, 11 β-strands and 22 coils. SeCKI had 19 potential antigenic epitopes and 25 HLA-I restricted epitopes. Based on phylogenetic analysis of SeCKI sequence, S. erinaceieuropaei has the closest evolutionary status with Hymenolepis microstoma. Information from this study could provide important insights into the identification of diagnostic antigens and molecular targets of anti-Sparganum drugs.
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Serodiagnosis of sparganosis by ELISA using recombinant cysteine protease of Spirometra erinaceieuropaei spargana
Parasitology Research, 2014Co-Authors: Xi Zhang, Peng Jiang, Jian Zhou, Rui Zhe He, Zhong Quan WangAbstract:The Spirometra erinaceieuropaei cysteine protease (SeCP) gene encoding a 36 kDa protein was expressed in Escherichia coli, and the potential of recombinant SeCP protein (rSeCP) as an antigen for the serodiagnosis of sparganosis was investigated by ELISA and compared with those of ELISA with Sparganum excretory–secretory (ES) antigens. The sensitivity of rSeCP-ELISA and ES-ELISA was 96.67 % (29/30) and 100 % (30/30) respectively, for the detection of anti-Sparganum IgG antibodies in sera of the experimentally infected mice (P > 0.05), and the specificities of both ELISA were 100 % (77/77). In heavily, moderately, and lightly infected mice (five, three, and one larvae per mouse), anti-Sparganum antibodies were firstly detected by rSeCP-ELISA at 10–12 days post-infection (dpi), respectively, and then continued to increase with a detection rate of 100 % at 14–22 dpi. In three groups of infected mice, the anti-Sparganum antibody levels at different times after infection were statistically different (P < 0.05). The results showed that the rSeCP might be a potential candidate antigen for early and specific serodiagnosis of sparganosis. But, it needs to be further evaluated with sera of the patients with sparganosis and other helminthiasis.
Jungwon Ju - One of the best experts on this subject based on the ideXlab platform.
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seropositivity and identification of paramyosin for sparganosis in the kangwon and incheon provinces of the republic of korea
Journal of Helminthology, 2017Co-Authors: Jungwon Ju, Hyewon Yang, Miyeoun ParkAbstract:Sparganosis is one of the top three tissue-dwelling heterologous helminthic diseases, along with cysticercosis and paragonimiasis, in Korea. Due to a lack of effective early diagnosis and treatment methods, this parasitic disease is regarded as a public health threat. This study evaluated reactivity, against Sparganum extracts, of sera from inhabitants of Cheorwon-gun, Goseong-gun and Ongjin-gun in Korea. The sera from 836 subjects were subjected to enzyme-linked immunosorbent assay and immunoblot analysis. The sera from 18 (5.8%) and 15 (5.1%) inhabitants in Cheorwon-gun ( n = 312) and Goseong-gun ( n = 294), respectively, exhibited highly positive reactions to the Sparganum antigen, whereas only two (0.9%) inhabitants in Ongjin-gun ( n = 230) showed positivity. We sought antigenic proteins for serodiagnosis of positive sera by immunoproteomic approaches. Total Sparganum lysates were separated by two-dimensional electrophoresis and then subjected to immunoblot analysis with mixed sparganosis-positive sera. We found seven antigenic spots and identified paramyosin as an antigenic protein by liquid chromatography–mass spectrometry. By two-dimensional (2D)-based mass analysis and immunoblotting against sparganosis-positive sera, paramyosin was identified as a candidate antigen for serodiagnosis of sparganosis.
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Transcriptome sequencing and analysis of the zoonotic parasite Spirometra erinacei spargana (plerocercoids)
Parasites & Vectors, 2014Co-Authors: Hyewon Yang, Jungwon JuAbstract:Background Although spargana, which are the plerocercoids of Spirometra erinacei , are of biological and clinical importance, expressed sequence tags (ESTs) from this parasite have not been explored. To understand molecular and biological features of this parasite, Sparganum ESTs were examined by large-scale EST sequencing and multiple bioinformatics tools. Methods Total RNA was isolated from spargana and then ESTs were generated, assembled and sequenced. Many biological aspects of spargana were investigated using multi-step bioinformatics tools. Results A total of 5,634 ESTs were collected from spargana. After clustering and assembly, the functions of 1,794 Sparganum Assembled ESTs (SpAEs) including 934 contigs and 860 singletons were analyzed. A total of 1,351 (75%) SpAEs were annotated using a hybrid of BLASTX and InterProScan. Of these genes, 1,041 (58%) SpAEs had high similarity to tapeworms. In the context of the biology of Sparganum, our analyses reveal: (i) a highly expressed fibronectin 1, a ubiquitous and abundant glycoprotein; (ii) up-regulation of enzymes related with glycolysis pathway; (iii) most frequent domains of protein kinase and RNA recognition motif domain; (iv) a set of helminth-parasitic and spargana-specific genes that may offer a number of antigen candidates. Conclusions Our transcriptomic analysis of S. erinacei spargana demonstrates biological aspects of a parasite that invades and travels through subcutaneous tissue in intermediate hosts. Future studies should include comparative analyses using combinations of transcriptome and proteome data collected from the entire life cycle of S. erinacei .
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spiroestdb a transcriptome database and online tool for Sparganum expressed sequences tags
BMC Research Notes, 2012Co-Authors: Hyewon Yang, Junhyung Park, Hongseog Park, Jungwon JuAbstract:Background Sparganum (plerocercoid of Spirometra erinacei) is a parasite that possesses the remarkable ability to survive by successfully modifying its physiology and morphology to suit various hosts and can be found in various tissues, even the nervous system. However, surprisingly little is known about the molecular function of genes that are expressed during the course of the parasite life cycle. To begin to decipher the molecular processes underlying gene function, we constructed a database of expressed sequence tags (ESTs) generated from Sparganum.
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SpiroESTdb: a transcriptome database and online tool for Sparganum expressed sequences tags
BMC Research Notes, 2012Co-Authors: Hyewon Yang, Junhyung Park, Hongseog Park, Jungwon JuAbstract:Background Sparganum (plerocercoid of Spirometra erinacei ) is a parasite that possesses the remarkable ability to survive by successfully modifying its physiology and morphology to suit various hosts and can be found in various tissues, even the nervous system. However, surprisingly little is known about the molecular function of genes that are expressed during the course of the parasite life cycle. To begin to decipher the molecular processes underlying gene function, we constructed a database of expressed sequence tags (ESTs) generated from Sparganum. Findings SpiroESTdb is a web-based information resource that is built upon the annotation and curation of 5,655 ESTs data. SpiroESTdb provides an integrated platform for expressed sequence data, expression dynamics, functional genes, genetic markers including single nucleotide polymorphisms and tandem repeats, gene ontology and KEGG pathway information. Moreover, SpiroESTdb supports easy access to gene pages, such as (i) curation and query forms, (ii) in silico expression profiling and (iii) BLAST search tools. Comprehensive descriptions of the Sparganum content of all sequenced data are available, including summary reports. The contents of SpiroESTdb can be viewed and downloaded from the web ( http://pathod.cdc.go.kr/spiroestdb ). Conclusions This integrative web-based database of sequence data, functional annotations and expression profiling data will serve as a useful tool to help understand and expand the characterization of parasitic infections. It can also be used to identify potential industrial drug targets and vaccine candidate genes.
Peng Jiang - One of the best experts on this subject based on the ideXlab platform.
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Large-scale survey of a neglected agent of sparganosis Spirometra erinaceieuropaei (Cestoda: Diphyllobothriidae) in wild frogs in China.
PLOS Neglected Tropical Diseases, 2020Co-Authors: Xi Zhang, Peng Jiang, Xiu Hong, Shu Chuan Zhao, Zhong Quan WangAbstract:Background In China, frogs play an understudied role in the spread of human sparganosis (caused by the larval form of Spirometra). However, our knowledge about the prevalence of Sparganum infection in frogs remains fragmented, and the taxonomic identification of the parasite is still controversial. Methodology/Principal findings The prevalence of Sparganum infection in wild frogs was surveyed at 145 geographical locations from 28 of the 34 provinces/autonomous regions/municipalities in China for six years. The collected Sparganum isolates from the different locations were subjected to molecular identification by a multiplex PCR assay and then were analysed with clustering analysis. In the survey, Sparganum infection was found in 8 out of 13 of the collected frog species, and the most frequently infected species was Pelophylax nigromaculatus (the infection rate was up to 14.07%). Infected frogs were found in 80 of the 145 surveyed locations. The Sparganum infection rates in the wild frogs in several regions of China were still high (above 10%), especially in South and Southwest China. A total of 72 spargana were selected for molecular identification, and the clustering analysis showed that sequences from the Chinese isolates were very similar to those identified as from Spirometra erinaceieuropaei. However, the taxonomy of the genus remains confused and further analysis is required. Conclusions Eating wild frogs is associated with considerable health risks in China. Several traditional Chinese folk remedies may increase the risk of infection. The Sparganum isolates in China are most likely from S. erinaceieuropaei, but new studies, especially comprehensive morphological analyses, are needed in the future.
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development of est derived microsatellite markers to investigate the population structure of Sparganum the causative agent of zoonotic sparganosis
Parasitology, 2019Co-Authors: Xi Zhang, Peng Jiang, Xiu Hong, Jiang Yang Duan, Zi Yang Hong, Zhong Quan WangAbstract:: The plerocercoid (Sparganum) of Spirometra erinaceieuropaei is the main aetiological agent of human sparganosis. To improve the current knowledge on S. erinaceieuropaei evolution, we performed multi-locus microsatellite typing of Sparganum isolates from China for the first time. All available expressed sequence tag (EST) sequences for the Spirometra were downloaded from the GenBank. The identification and localization of microsatellites in ESTs was accomplished by MISA. Based on the selected microsatellites, the genetic structure of 64 Sparganum isolates collected from 11 geographical locations in southwest China were investigated through principal component analysis, STRUCTURE analysis and neighbour-joining clustering. A total of 522 non-redundant ESTs containing 915 simple sequence repeats were identified from 12 481 ESTs screened. Five primer pairs were finally selected. Using these loci, a total of 12 alleles were detected in 64 Sparganum isolates. Little variability was observed within each of geographical population, especially among isolates derived from Kunming of Yunnan (YN-KM) province. Both STRUCTURE analysis and the clustering analysis supported that two genotypes existed among the Sparganum isolates from southwest China. In conclusion, five microsatellite markers were successfully developed, and Sparganum population was observed to harbour low genetic variation, further investigation with deeper sampling was needed to elucidate the population structure.
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The phylogenetic diversity of Spirometra erinaceieuropaei isolates from southwest China revealed by multi genes.
Acta Tropica, 2016Co-Authors: Xi Zhang, Peng Jiang, Han Wang, Yun Lu Zhang, Zhong Quan WangAbstract:The larval plerocercoid of Spirometra erinaceieuropaei can parasitize humans, causing a serious food borne parasitic zoonosis known as sparganosis. Sparganosis have increased in China in recent years. In this study, the prevalence of Sparganum infection in wild frogs in 9 geographical areas in southwest China was firstly investigated. Of 276 caught frogs, 55 frogs were found to be infected with Sparganum. Then, the population genetic structure of these Sparganum isolates was explored based on four molecular markers (cytb, cox1, rrnS and 28S rDNA D1). Highly genetic diversity and the genetic differentiation among Sparganum isolates from different sites were revealed in the DNA polymorphism analyses. Both the phylogenetic inference and the analysis of the median-joining network supported two clades in the southwest S. erinaceieuropaei population. However, none demographic population expansion of the southwest S. erinaceieuropaei population was observed in the neutrality test, mismatch distribution analysis and Bayesian skyline plot analysis. Finally, the phylogenetic diversity of S. erinaceieuropaei from eastern, central, southern and southwest China was analyzed, the result suggested that Chinese S. erinaceieuropaei population should be divided into two groups (Group I and Group II), and they started to divergence in the middle Pliocene.
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levels of Sparganum infections and phylogenetic analysis of the tapeworm spirometra erinaceieuropaei Sparganum in wild frogs from henan province in central china
Journal of Helminthology, 2015Co-Authors: X Zhang, Peng Jiang, Z Q WangAbstract:Sparganosis is a serious food-borne parasitic zoonosis caused by infection with Spirometra spargana. The prevalence of Sparganum infection in wild frogs ( Rana nigromaculata , R. limmochari , R. temporaria and Bufo gargarizans ) was investigated in Henan Province of central China during 2008–2012. Of 3482 caught wild frogs, 565 (16.23%) were found to be infected with plerocercoids (spargana) of the genus Spirometra . Spargana were found in 14.85% (320/2155) of R. nigromaculata , 20.82% (233/1119) of R. limmochari and 10.91% (12/110) of R. temporaria frogs. However, no Sparganum was found in B. gargarizans . To investigate the phylogenetic position of collected spargana, three mitochondrial DNA (mtDNA) regions, namely cytochrome c oxidase subunits 1 and 3 ( cox 1 and cox 3), and NADH dehydrogenase subunit 4 ( nad 4), were amplified, sequenced and analysed. Sequences of cox 1, cox 3 and p nad 4 were 417, 390 and 578 bp in length, respectively. The base composition of cox 1, cox 3 and p nad 4 were generally AT rich with a mean of 63.5%, 68.3% and 67% AT, respectively. Phylogenetic analysis showed that all the Sparganum isolates in Henan Province represented Spirometra erinaceieuropaei and were a well-supported clade. These findings demonstrated clearly the usefulness of the three mtDNA sequences for molecular identification and population genetics studies of S. erinaceieuropaei spargana of human and animal health significance.
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Analysis of structure, function and epitopes of Spirometra erinaceieuropaei casein kinase I.
Tropical Biomedicine, 2015Co-Authors: Zhong Quan Wang, Zi Fang Zhang, Peng Jiang, Xi Zhang, G Y ZhangAbstract:: Spirometra erinaceieuropaei casein kinase I (SeCKI) was analyzed using bioinformatical methods to predict its structure and function based on the deduced amino acid sequence from full length cDNA sequence of SeCKI gene with online sites and software programs. The longest open reading frame contains 448 amino acids, 50 kDa and theoretical pI of 4.73, with a complete tubulin domain, a SMART tubulin_C domain and a low complexity region. SeCKI has no signal sequence and no transmembrane domain, but is predicted to be located extracellularly. The secondary structure of SeCKI contains 12 α-helixes, 11 β-strands and 22 coils. SeCKI had 19 potential antigenic epitopes and 25 HLA-I restricted epitopes. Based on phylogenetic analysis of SeCKI sequence, S. erinaceieuropaei has the closest evolutionary status with Hymenolepis microstoma. Information from this study could provide important insights into the identification of diagnostic antigens and molecular targets of anti-Sparganum drugs.
Hyewon Yang - One of the best experts on this subject based on the ideXlab platform.
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seropositivity and identification of paramyosin for sparganosis in the kangwon and incheon provinces of the republic of korea
Journal of Helminthology, 2017Co-Authors: Jungwon Ju, Hyewon Yang, Miyeoun ParkAbstract:Sparganosis is one of the top three tissue-dwelling heterologous helminthic diseases, along with cysticercosis and paragonimiasis, in Korea. Due to a lack of effective early diagnosis and treatment methods, this parasitic disease is regarded as a public health threat. This study evaluated reactivity, against Sparganum extracts, of sera from inhabitants of Cheorwon-gun, Goseong-gun and Ongjin-gun in Korea. The sera from 836 subjects were subjected to enzyme-linked immunosorbent assay and immunoblot analysis. The sera from 18 (5.8%) and 15 (5.1%) inhabitants in Cheorwon-gun ( n = 312) and Goseong-gun ( n = 294), respectively, exhibited highly positive reactions to the Sparganum antigen, whereas only two (0.9%) inhabitants in Ongjin-gun ( n = 230) showed positivity. We sought antigenic proteins for serodiagnosis of positive sera by immunoproteomic approaches. Total Sparganum lysates were separated by two-dimensional electrophoresis and then subjected to immunoblot analysis with mixed sparganosis-positive sera. We found seven antigenic spots and identified paramyosin as an antigenic protein by liquid chromatography–mass spectrometry. By two-dimensional (2D)-based mass analysis and immunoblotting against sparganosis-positive sera, paramyosin was identified as a candidate antigen for serodiagnosis of sparganosis.
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Transcriptome sequencing and analysis of the zoonotic parasite Spirometra erinacei spargana (plerocercoids)
Parasites & Vectors, 2014Co-Authors: Hyewon Yang, Jungwon JuAbstract:Background Although spargana, which are the plerocercoids of Spirometra erinacei , are of biological and clinical importance, expressed sequence tags (ESTs) from this parasite have not been explored. To understand molecular and biological features of this parasite, Sparganum ESTs were examined by large-scale EST sequencing and multiple bioinformatics tools. Methods Total RNA was isolated from spargana and then ESTs were generated, assembled and sequenced. Many biological aspects of spargana were investigated using multi-step bioinformatics tools. Results A total of 5,634 ESTs were collected from spargana. After clustering and assembly, the functions of 1,794 Sparganum Assembled ESTs (SpAEs) including 934 contigs and 860 singletons were analyzed. A total of 1,351 (75%) SpAEs were annotated using a hybrid of BLASTX and InterProScan. Of these genes, 1,041 (58%) SpAEs had high similarity to tapeworms. In the context of the biology of Sparganum, our analyses reveal: (i) a highly expressed fibronectin 1, a ubiquitous and abundant glycoprotein; (ii) up-regulation of enzymes related with glycolysis pathway; (iii) most frequent domains of protein kinase and RNA recognition motif domain; (iv) a set of helminth-parasitic and spargana-specific genes that may offer a number of antigen candidates. Conclusions Our transcriptomic analysis of S. erinacei spargana demonstrates biological aspects of a parasite that invades and travels through subcutaneous tissue in intermediate hosts. Future studies should include comparative analyses using combinations of transcriptome and proteome data collected from the entire life cycle of S. erinacei .
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spiroestdb a transcriptome database and online tool for Sparganum expressed sequences tags
BMC Research Notes, 2012Co-Authors: Hyewon Yang, Junhyung Park, Hongseog Park, Jungwon JuAbstract:Background Sparganum (plerocercoid of Spirometra erinacei) is a parasite that possesses the remarkable ability to survive by successfully modifying its physiology and morphology to suit various hosts and can be found in various tissues, even the nervous system. However, surprisingly little is known about the molecular function of genes that are expressed during the course of the parasite life cycle. To begin to decipher the molecular processes underlying gene function, we constructed a database of expressed sequence tags (ESTs) generated from Sparganum.
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SpiroESTdb: a transcriptome database and online tool for Sparganum expressed sequences tags
BMC Research Notes, 2012Co-Authors: Hyewon Yang, Junhyung Park, Hongseog Park, Jungwon JuAbstract:Background Sparganum (plerocercoid of Spirometra erinacei ) is a parasite that possesses the remarkable ability to survive by successfully modifying its physiology and morphology to suit various hosts and can be found in various tissues, even the nervous system. However, surprisingly little is known about the molecular function of genes that are expressed during the course of the parasite life cycle. To begin to decipher the molecular processes underlying gene function, we constructed a database of expressed sequence tags (ESTs) generated from Sparganum. Findings SpiroESTdb is a web-based information resource that is built upon the annotation and curation of 5,655 ESTs data. SpiroESTdb provides an integrated platform for expressed sequence data, expression dynamics, functional genes, genetic markers including single nucleotide polymorphisms and tandem repeats, gene ontology and KEGG pathway information. Moreover, SpiroESTdb supports easy access to gene pages, such as (i) curation and query forms, (ii) in silico expression profiling and (iii) BLAST search tools. Comprehensive descriptions of the Sparganum content of all sequenced data are available, including summary reports. The contents of SpiroESTdb can be viewed and downloaded from the web ( http://pathod.cdc.go.kr/spiroestdb ). Conclusions This integrative web-based database of sequence data, functional annotations and expression profiling data will serve as a useful tool to help understand and expand the characterization of parasitic infections. It can also be used to identify potential industrial drug targets and vaccine candidate genes.
Oscar Noya - One of the best experts on this subject based on the ideXlab platform.
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phylogenetic identification of Sparganum proliferum as a pseudophyllidean cestode by the sequence analyses on mitochondrial coi and nuclear sdhb genes
Parasitology International, 2001Co-Authors: Hiroko Miyadera, Oscar Noya, Belkisyolealarcon De Noya, Akatsuki Kokaze, Kiyoshi Kita, Rikuo Machinami, Munehiro Okamoto, Toshiaki Kuramochi, Somei KojimaAbstract:Abstract Sparganum proliferum is a larval cestode for which the adult stage is unknown. It is characterized by the continuous branching and budding when parasitized to humans, and causes fatal human sparganosis. However, the biological features of S. proliferum, including its taxonomic status, still remain obscure. Our previous investigation suggested that S. proliferum might be phylogenetically distinct from Spirometra erinaceieuropaei, by the analysis on mitochondrial NADH dehydrogenase subunit 3 (ND3) gene. However, mitochondrial DNA sequence in Platyhelminth is known to have heteroplasmy within a species. Therefore, in the present study, we have investigated the complete nucleotide sequences of mitochondrial cytochrome c oxidase subunit I (COI) gene and the partial nucleotide sequences of nuclear coded succinate dehydrogenase iron–sulfur protein subunit gene (sdhB). The results clearly demonstrated that S. proliferum is a distinct species from S. erinaceieuropaei, and that S. proliferum belongs to the order Pseudophyllidea.
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phylogenetic identification of Sparganum proliferum as a pseudophyllidean cestode
Parasitology International, 1997Co-Authors: Akatsuki Kokaze, Oscar Noya, Belkisyolealarcon De Noya, Hiroko Miyadera, Kiyoshi Kita, Rikuo Machinami, Munehiro Okamoto, Toshihiro Horii, Somei KojimaAbstract:Abstract Sparganum proliferum is characterized by continuous branching and budding, the resulting progeny invading all tissues of the human body, causing fatal sparganosis. Its life cycle, definitive hosts and the route of infection to humans have not yet been disclosed. Because its morphology is similar to Spirometra erinacei, the phylogeny of S. proliferum has been thought to be identical to or closely related to S. erinacei. However, the taxonomy of S. proliferum has not been established up to present due to the lack of definitive observations. In order to clarify the phylogenetic relationship between S. proliferum and S. erinacei, nucleotide sequences of mitochondrial NADH dehydrogenase subunit 3 gene (ND3) and four mitochondrial tRNA coding genes of S. proliferum and other pseudophyllidean cestodes were analyzed. The sequences of S. proliferum showed high similarity to those of S. erinacei, although they were clearly different from each other, indicating that the phylogeny of S. proliferum and S. erinacei is distinct. This is the first report showing the phylogenetic relationship among S. proliferum and other pseudophyllidean cestodes at the DNA sequence level.
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maintenance of Sparganum proliferum in vitro and in experimental animals
International Journal for Parasitology, 1992Co-Authors: Alarcon B De Noya, Jaime R Torres, Oscar NoyaAbstract:Abstract Successful in vitro and in vivo maintenance of Sparganum proliferum is described for the first time. Various experimental animals including hamsters, mice and a monkey were evaluated. Albino mice inoculated either subcutaneously or intraperitoneally allowed the survival and multiplication of larvae for as long as 72 weeks. Intensity of infection was proportional to the length of exposure; however, the number of larvae collected from inoculated animals varied widely when infection lasted for 6 or more months. Inoculation of single larval segments appears as effective as that of complete larvae. Although Minimal Essential Medium allowed the survival of S. proliferum for as long as 14 weeks, growth was observed only during the first 4 weeks of culturing. Despite initial in vitro growth of larvae, neither differentiation into a more developed stage nor multiplication was obtained.
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Sparganum proliferum an overview of its structure and ultrastructure
International Journal for Parasitology, 1992Co-Authors: Oscar Noya, Belkisyolealarcon De Noya, Hector Arrechedera, Jaime R Torres, Carlos ArguelloAbstract:Abstract Noya O. , Alarcon De Noya B. , Arrechedera H. , Torres J. and Arguello C. 1992. Sparganum proliferum : an overview of its structure and ultrastructure. International Journal for Parasitology 22 : 631–640. A detailed study of the structure and ultrastructure of Sparganum proliferum was made possible for the first time thanks to the successful in vitro and in vivo maintenance of this rare parasite. Although S. proliferum exhibits many of the classical tegumental and parenchymal structures previously described for other larval cestodes, these are either arranged in a distinct fashion or, in some cases, may be completely different. Among the latter and of special interest are the single or multiple parenchymal cavities, surrounded by tegument, which in some instances appear to act as a primitive digestive tract.