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Ching-shwun Lin - One of the best experts on this subject based on the ideXlab platform.
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label retaining and Stem Cell Marker expression in the developing rat urinary bladder
Urology, 2012Co-Authors: Guiting Lin, Lia Banie, Hongxiu Ning, Tom F. Lue, Haiyang Zhang, Xuefeng Qiu, Ching-shwun LinAbstract:Objective To identify potential Stem Cells in urinary bladder by label-retaining Cell (LRC) strategy and immunostaining for putative Stem Cell Markers. Methods Newborn rats were intraperitoneally injected with 5-ethynyl-2-deoxyuridine (EdU) and their bladders harvested at 4 different time points afterward. The bladders were processed for EdU staining and immunofluorescence staining for Stem Cell Markers Lgr5, CD34, SSEA-1, and c-kit. EdU-positive Cells were counted and colocalization with Stem Cell Markers determined. Results At day 1 post-EdU injection, 1804.0 ± 227.7 bladder Cells were labeled in each cross-section. As time increased, fewer bladders remained labeled, dropping to 236.5 ± 53.0 Cells per field. In the 1-day bladders, 27.5% ± 4.9% of the epithelial Cells were labeled as compared to 12.1% ± 2.8% in the detrusor. The labeling rates in these 2 tissue compartments gradually equalized, reaching at approximately 5.5% in the 8-week samples. Distribution of LRC was random, without preferential labeling of basal Cells. Lgr5 and SSEA-1 were detectable in the urothelium, and CD34 and c-kit in the lamina propria and detrusor. Approximately 30%-40% of c-kit–positive Cells were EdU positive. Conclusion Labeling of bladder Cells by EdU occurred randomly, and label retaining was not associated with expression of Lgr5, CD34, or SSEA-1. The strong association between label retaining and c-kit expression appears to relate to interstitial Cells of Cajal, not Stem Cells.
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Label retaining and Stem Cell Marker expression in the developing rat urinary bladder.
Urology, 2011Co-Authors: Haiyang Zhang, Guiting Lin, Lia Banie, Hongxiu Ning, Tom F. Lue, Xuefeng Qiu, Ching-shwun LinAbstract:To identify potential Stem Cells in urinary bladder by label-retaining Cell (LRC) strategy and immunostaining for putative Stem Cell Markers. Newborn rats were intraperitoneally injected with 5-ethynyl-2-deoxyuridine (EdU) and their bladders harvested at 4 different time points afterward. The bladders were processed for EdU staining and immunofluorescence staining for Stem Cell Markers Lgr5, CD34, SSEA-1, and c-kit. EdU-positive Cells were counted and colocalization with Stem Cell Markers determined. At day 1 post-EdU injection, 1804.0 ± 227.7 bladder Cells were labeled in each cross-section. As time increased, fewer bladders remained labeled, dropping to 236.5 ± 53.0 Cells per field. In the 1-day bladders, 27.5% ± 4.9% of the epithelial Cells were labeled as compared to 12.1% ± 2.8% in the detrusor. The labeling rates in these 2 tissue compartments gradually equalized, reaching at approximately 5.5% in the 8-week samples. Distribution of LRC was random, without preferential labeling of basal Cells. Lgr5 and SSEA-1 were detectable in the urothelium, and CD34 and c-kit in the lamina propria and detrusor. Approximately 30%-40% of c-kit-positive Cells were EdU positive. Labeling of bladder Cells by EdU occurred randomly, and label retaining was not associated with expression of Lgr5, CD34, or SSEA-1. The strong association between label retaining and c-kit expression appears to relate to interstitial Cells of Cajal, not Stem Cells. Copyright © 2012 Elsevier Inc. All rights reserved.
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mesenchymal Stem Cell Marker stro 1 is a 75kd endothelial antigen
Biochemical and Biophysical Research Communications, 2011Co-Authors: Hongxiu Ning, Guiting Lin, Tom F. Lue, Ching-shwun LinAbstract:Stro-1 is the best-known mesenchymal Stem Cell (MSC) Marker. However, previous studies have observed its expression in the endothelium. In the present study we performed immunofluorescence (IF) staining for Stro-1, using endothelial Marker vWF as reference. In the liver, both proteins were expressed in the endothelium of the central veins and hepatic sinusoids. In the lung, both were expressed in the endothelium of pulmonary blood vessels, but while vWF was absent in the alveolar capillaries, Stro-1 was present. In the kidney, both were expressed in the endothelium of renal arterial branches, but while vWF was strongly expressed in the glomeruli, Stro-1 only scantly. IF staining in cultured endothelial Cells also showed extensive overlaps between Stro-1 and vWF. Western blot analysis with Stro-1 antibody detected a single protein band of 75kd in endothelial Cells but not smooth muscle Cells, fibroblasts, or B Cells. Cancer Cell lines PC3, DU145, MCF7, and K562 were also positive. Adipose-derived Stem Cells (ADSCs) expressed higher levels of Stro-1 when cultured beyond the first passage or when induced to differentiate into endothelial Cells. These data, together with previous studies, indicate that Stro-1 is intrinsically an endothelial antigen, and its expression in MSC is probably an induced event.
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Tissue distribution of mesenchymal Stem Cell Marker Stro-1.
Stem cells and development, 2011Co-Authors: Guiting Lin, Gang Liu, Lia Banie, Guifang Wang, Hongxiu Ning, Tom F. Lue, Ching-shwun LinAbstract:Stro-1 is the best-known mesenchymal Stem Cell Marker. However, despite its bone marrow origin, its localization in bone marrow has never been demonstrated. By immunofluorescence staining, we show here that ∼0.74% of nucleated bone morrow Cells expressed Stro-1. We also found that ∼8.7% of CD34-expressing Cells expressed Stro-1, and more than 20% of Stro-1-expressing Cells did not express CD34. In adipose tissue Stro-1 expression was identified in the endothelium of arterioles and capillaries. Stro-1 was also localized in the endothelium of some but not all adipose tissue veins. Endothelial expression of Stro-1 was also identified in blood vessels in penis and in leg muscles, but not in other tested tissues. In these other tissues, Stro-1 was scantly expressed near but not in blood vessels. These variable and endothelial expression patterns of Stro-1 point to a need to re-examine published data that relied on Stro-1 as a mesenchymal Stem Cell Marker.
Maria Grazia Narducci - One of the best experts on this subject based on the ideXlab platform.
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the tcl1 oncogene defines secondary hair germ Cells differentiation at catagen telogen transition and affects Stem Cell Marker cd34 expression
Oncogene, 2009Co-Authors: Gianluca Ragone, Antonella Bresin, F Piermarini, Cristina Lazzeri, Maria Cristina Picchio, Sumin Kang, Carlo M. Croce, Max D Cooper, Daniele Remotti, Maria Grazia NarducciAbstract:The Tcl1 oncogene defines secondary hair germ Cells differentiation at catagen–telogen transition and affects Stem-Cell Marker CD34 expression
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The Tcl1 oncogene defines secondary hair germ Cells differentiation at catagen–telogen transition and affects Stem-Cell Marker CD34 expression
Oncogene, 2009Co-Authors: Gianluca Ragone, Antonella Bresin, F Piermarini, Cristina Lazzeri, Maria Cristina Picchio, Carlo M. Croce, S. M. Kang, Max D Cooper, Daniele Remotti, Maria Grazia NarducciAbstract:Overexpression of the TCL1 gene family plays a role in the onset of T-Cell leukemias in mice and in humans. The Tcl1 gene is tightly regulated during early embryogenesis in which it participates in embryonic Stem (ES)-Cells proliferation and during lymphoid differentiation. Here, we provide evidences that Tcl1 is also important in mouse hair follicle (HF) and skin homeostasis. We found that Tcl1 ^ −/− adult mice exhibit hair loss, leading to alopecia with extensive skin lesions. By analysing Tcl1 expression in the wild-type (wt) skin through different stages of hair differentiation, we observe high levels in the secondary hair germ (HG) Cells and hair bulges, during early anagen and catagen–telogen transition phases. The loss of Tcl1 does not result in apparent skin morphological defects during embryonic development and at birth, but its absence causes a reduction of proliferation in anagen HFs. Importantly, we show the that absence of Tcl1 induces a significant loss of the Stem-Cell Marker CD34 (but not α6-integrin) expression in the bulge Cells, which is necessary to maintain Stem-Cell characteristics. Therefore, our findings indicate that Tcl1 gene(s) might have important roles in hair formation, by its involvement in cycling and self-renewal of transient amplifying (TA) and Stem-Cell (SC) populations.
Toshio Miki - One of the best experts on this subject based on the ideXlab platform.
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Quantitative comparison of Stem Cell Marker-positive Cells in fetal and term human amnion
Journal of reproductive immunology, 2009Co-Authors: Masanori Izumi, Benjamin J. Pazin, Crescenzio Francesco Minervini, Jörg C. Gerlach, Mark A. Ross, Donna B. Stolz, Morris E. Turner, Robert L. Thompson, Toshio MikiAbstract:Abstract Scattered in the amniotic epithelium of the human term placenta are pluripotent Stem Cell Marker-positive Cells. Unlike other parts of the placenta, amniotic epithelial (AE) Cells are derived from pluripotent epiblasts. It is hypothesized that most epiblast-derived fetal AE Cells are positive for Stem Cell Markers at the beginning of pregnancy and that the Stem Cell Marker-positive Cells scattered through the term amnion are remaining, epiblast-like Stem Cells. To test this hypothesis, human fetal amnia from early-stage pregnancies were evaluated for expression of the Stem Cell-specific Cell surface Markers TRA 1-60 and TRA 1-81 and of the pluripotent Stem Cell Marker genes Oct4, Nanog, and Sox2. Whole-mount immunohistochemical analysis demonstrated that a greater proportion of AE Cells in the 17–19 week human fetal amnion are positive for both TRA 1-60 and TRA 1-81 than in the term amnion. Quantitative real-time RT-PCR analysis confirmed that the fetal AE Cells exhibit greater Stem Cell Marker gene expression than those in term placentae. Expression of both Nanog and Sox2 mRNAs were significantly higher in the fetal amnion, while Oct4 mRNA expression was not significantly changed. These differences in abundance of Stem Cell Marker-positive Cells and Stem Cell Marker gene expression together indicate that some Stem Cell Marker-positive Cells are conserved over the course of pregnancy. The results suggest that Stem Cell Marker-positive AE Cells in the term amnion are retained from epiblast-derived fetal AE Cells.
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Identification of Stem Cell Marker-positive Cells by immunofluorescence in term human amnion.
Journal of reproductive immunology, 2007Co-Authors: Toshio Miki, Mark A. Ross, Donna B. Stolz, Keitaro Mitamura, Stephen C. StromAbstract:The placenta contains different populations of Stem/progenitor Cells such as mesenchymal, hematopoietic, trophoblastic and pluripotent Stem Cells. Although some tissue-specific Stem Cells are restricted to particular parts of the placenta, the localization of embryonic Stem Cell-like Cells in term human placenta has not been determined. We have used immunofluorescence staining techniques with antibodies to pluripotent Stem Cell antigens, SSEA-3, SSEA-4, TRA 1-60 and TRA 1-81, and confocal microscopic analysis to identify and localize Stem Cells within the placenta. Stem Cell Marker-positive Cells were found in amnion but not in choriodecidua, tissues known to contain hematopoietic and trophoblastic Stem Cells. Amniotic mesenchymal Cells did not react with these pluripotent Stem Cell Markers, while all amniotic epithelial Cells reacted with at least one antibody. The TRA 1-60 and TRA 1-81 positive Cells were solitary and present throughout the surface of amniotic membrane without a specific pattern of distribution, whereas SSEA-3 was negative and SSEA-4 was weakly positive on all amniotic epithelial Cells. These data suggest that the human amnion contains Stem Cell-like Cells at different states of differentiation. Human term amnion may be useful source of pluripotent Stem Cells for regenerative medicine.
Hongxiu Ning - One of the best experts on this subject based on the ideXlab platform.
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label retaining and Stem Cell Marker expression in the developing rat urinary bladder
Urology, 2012Co-Authors: Guiting Lin, Lia Banie, Hongxiu Ning, Tom F. Lue, Haiyang Zhang, Xuefeng Qiu, Ching-shwun LinAbstract:Objective To identify potential Stem Cells in urinary bladder by label-retaining Cell (LRC) strategy and immunostaining for putative Stem Cell Markers. Methods Newborn rats were intraperitoneally injected with 5-ethynyl-2-deoxyuridine (EdU) and their bladders harvested at 4 different time points afterward. The bladders were processed for EdU staining and immunofluorescence staining for Stem Cell Markers Lgr5, CD34, SSEA-1, and c-kit. EdU-positive Cells were counted and colocalization with Stem Cell Markers determined. Results At day 1 post-EdU injection, 1804.0 ± 227.7 bladder Cells were labeled in each cross-section. As time increased, fewer bladders remained labeled, dropping to 236.5 ± 53.0 Cells per field. In the 1-day bladders, 27.5% ± 4.9% of the epithelial Cells were labeled as compared to 12.1% ± 2.8% in the detrusor. The labeling rates in these 2 tissue compartments gradually equalized, reaching at approximately 5.5% in the 8-week samples. Distribution of LRC was random, without preferential labeling of basal Cells. Lgr5 and SSEA-1 were detectable in the urothelium, and CD34 and c-kit in the lamina propria and detrusor. Approximately 30%-40% of c-kit–positive Cells were EdU positive. Conclusion Labeling of bladder Cells by EdU occurred randomly, and label retaining was not associated with expression of Lgr5, CD34, or SSEA-1. The strong association between label retaining and c-kit expression appears to relate to interstitial Cells of Cajal, not Stem Cells.
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Label retaining and Stem Cell Marker expression in the developing rat urinary bladder.
Urology, 2011Co-Authors: Haiyang Zhang, Guiting Lin, Lia Banie, Hongxiu Ning, Tom F. Lue, Xuefeng Qiu, Ching-shwun LinAbstract:To identify potential Stem Cells in urinary bladder by label-retaining Cell (LRC) strategy and immunostaining for putative Stem Cell Markers. Newborn rats were intraperitoneally injected with 5-ethynyl-2-deoxyuridine (EdU) and their bladders harvested at 4 different time points afterward. The bladders were processed for EdU staining and immunofluorescence staining for Stem Cell Markers Lgr5, CD34, SSEA-1, and c-kit. EdU-positive Cells were counted and colocalization with Stem Cell Markers determined. At day 1 post-EdU injection, 1804.0 ± 227.7 bladder Cells were labeled in each cross-section. As time increased, fewer bladders remained labeled, dropping to 236.5 ± 53.0 Cells per field. In the 1-day bladders, 27.5% ± 4.9% of the epithelial Cells were labeled as compared to 12.1% ± 2.8% in the detrusor. The labeling rates in these 2 tissue compartments gradually equalized, reaching at approximately 5.5% in the 8-week samples. Distribution of LRC was random, without preferential labeling of basal Cells. Lgr5 and SSEA-1 were detectable in the urothelium, and CD34 and c-kit in the lamina propria and detrusor. Approximately 30%-40% of c-kit-positive Cells were EdU positive. Labeling of bladder Cells by EdU occurred randomly, and label retaining was not associated with expression of Lgr5, CD34, or SSEA-1. The strong association between label retaining and c-kit expression appears to relate to interstitial Cells of Cajal, not Stem Cells. Copyright © 2012 Elsevier Inc. All rights reserved.
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mesenchymal Stem Cell Marker stro 1 is a 75kd endothelial antigen
Biochemical and Biophysical Research Communications, 2011Co-Authors: Hongxiu Ning, Guiting Lin, Tom F. Lue, Ching-shwun LinAbstract:Stro-1 is the best-known mesenchymal Stem Cell (MSC) Marker. However, previous studies have observed its expression in the endothelium. In the present study we performed immunofluorescence (IF) staining for Stro-1, using endothelial Marker vWF as reference. In the liver, both proteins were expressed in the endothelium of the central veins and hepatic sinusoids. In the lung, both were expressed in the endothelium of pulmonary blood vessels, but while vWF was absent in the alveolar capillaries, Stro-1 was present. In the kidney, both were expressed in the endothelium of renal arterial branches, but while vWF was strongly expressed in the glomeruli, Stro-1 only scantly. IF staining in cultured endothelial Cells also showed extensive overlaps between Stro-1 and vWF. Western blot analysis with Stro-1 antibody detected a single protein band of 75kd in endothelial Cells but not smooth muscle Cells, fibroblasts, or B Cells. Cancer Cell lines PC3, DU145, MCF7, and K562 were also positive. Adipose-derived Stem Cells (ADSCs) expressed higher levels of Stro-1 when cultured beyond the first passage or when induced to differentiate into endothelial Cells. These data, together with previous studies, indicate that Stro-1 is intrinsically an endothelial antigen, and its expression in MSC is probably an induced event.
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Tissue distribution of mesenchymal Stem Cell Marker Stro-1.
Stem cells and development, 2011Co-Authors: Guiting Lin, Gang Liu, Lia Banie, Guifang Wang, Hongxiu Ning, Tom F. Lue, Ching-shwun LinAbstract:Stro-1 is the best-known mesenchymal Stem Cell Marker. However, despite its bone marrow origin, its localization in bone marrow has never been demonstrated. By immunofluorescence staining, we show here that ∼0.74% of nucleated bone morrow Cells expressed Stro-1. We also found that ∼8.7% of CD34-expressing Cells expressed Stro-1, and more than 20% of Stro-1-expressing Cells did not express CD34. In adipose tissue Stro-1 expression was identified in the endothelium of arterioles and capillaries. Stro-1 was also localized in the endothelium of some but not all adipose tissue veins. Endothelial expression of Stro-1 was also identified in blood vessels in penis and in leg muscles, but not in other tested tissues. In these other tissues, Stro-1 was scantly expressed near but not in blood vessels. These variable and endothelial expression patterns of Stro-1 point to a need to re-examine published data that relied on Stro-1 as a mesenchymal Stem Cell Marker.
Guiting Lin - One of the best experts on this subject based on the ideXlab platform.
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label retaining and Stem Cell Marker expression in the developing rat urinary bladder
Urology, 2012Co-Authors: Guiting Lin, Lia Banie, Hongxiu Ning, Tom F. Lue, Haiyang Zhang, Xuefeng Qiu, Ching-shwun LinAbstract:Objective To identify potential Stem Cells in urinary bladder by label-retaining Cell (LRC) strategy and immunostaining for putative Stem Cell Markers. Methods Newborn rats were intraperitoneally injected with 5-ethynyl-2-deoxyuridine (EdU) and their bladders harvested at 4 different time points afterward. The bladders were processed for EdU staining and immunofluorescence staining for Stem Cell Markers Lgr5, CD34, SSEA-1, and c-kit. EdU-positive Cells were counted and colocalization with Stem Cell Markers determined. Results At day 1 post-EdU injection, 1804.0 ± 227.7 bladder Cells were labeled in each cross-section. As time increased, fewer bladders remained labeled, dropping to 236.5 ± 53.0 Cells per field. In the 1-day bladders, 27.5% ± 4.9% of the epithelial Cells were labeled as compared to 12.1% ± 2.8% in the detrusor. The labeling rates in these 2 tissue compartments gradually equalized, reaching at approximately 5.5% in the 8-week samples. Distribution of LRC was random, without preferential labeling of basal Cells. Lgr5 and SSEA-1 were detectable in the urothelium, and CD34 and c-kit in the lamina propria and detrusor. Approximately 30%-40% of c-kit–positive Cells were EdU positive. Conclusion Labeling of bladder Cells by EdU occurred randomly, and label retaining was not associated with expression of Lgr5, CD34, or SSEA-1. The strong association between label retaining and c-kit expression appears to relate to interstitial Cells of Cajal, not Stem Cells.
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Label retaining and Stem Cell Marker expression in the developing rat urinary bladder.
Urology, 2011Co-Authors: Haiyang Zhang, Guiting Lin, Lia Banie, Hongxiu Ning, Tom F. Lue, Xuefeng Qiu, Ching-shwun LinAbstract:To identify potential Stem Cells in urinary bladder by label-retaining Cell (LRC) strategy and immunostaining for putative Stem Cell Markers. Newborn rats were intraperitoneally injected with 5-ethynyl-2-deoxyuridine (EdU) and their bladders harvested at 4 different time points afterward. The bladders were processed for EdU staining and immunofluorescence staining for Stem Cell Markers Lgr5, CD34, SSEA-1, and c-kit. EdU-positive Cells were counted and colocalization with Stem Cell Markers determined. At day 1 post-EdU injection, 1804.0 ± 227.7 bladder Cells were labeled in each cross-section. As time increased, fewer bladders remained labeled, dropping to 236.5 ± 53.0 Cells per field. In the 1-day bladders, 27.5% ± 4.9% of the epithelial Cells were labeled as compared to 12.1% ± 2.8% in the detrusor. The labeling rates in these 2 tissue compartments gradually equalized, reaching at approximately 5.5% in the 8-week samples. Distribution of LRC was random, without preferential labeling of basal Cells. Lgr5 and SSEA-1 were detectable in the urothelium, and CD34 and c-kit in the lamina propria and detrusor. Approximately 30%-40% of c-kit-positive Cells were EdU positive. Labeling of bladder Cells by EdU occurred randomly, and label retaining was not associated with expression of Lgr5, CD34, or SSEA-1. The strong association between label retaining and c-kit expression appears to relate to interstitial Cells of Cajal, not Stem Cells. Copyright © 2012 Elsevier Inc. All rights reserved.
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mesenchymal Stem Cell Marker stro 1 is a 75kd endothelial antigen
Biochemical and Biophysical Research Communications, 2011Co-Authors: Hongxiu Ning, Guiting Lin, Tom F. Lue, Ching-shwun LinAbstract:Stro-1 is the best-known mesenchymal Stem Cell (MSC) Marker. However, previous studies have observed its expression in the endothelium. In the present study we performed immunofluorescence (IF) staining for Stro-1, using endothelial Marker vWF as reference. In the liver, both proteins were expressed in the endothelium of the central veins and hepatic sinusoids. In the lung, both were expressed in the endothelium of pulmonary blood vessels, but while vWF was absent in the alveolar capillaries, Stro-1 was present. In the kidney, both were expressed in the endothelium of renal arterial branches, but while vWF was strongly expressed in the glomeruli, Stro-1 only scantly. IF staining in cultured endothelial Cells also showed extensive overlaps between Stro-1 and vWF. Western blot analysis with Stro-1 antibody detected a single protein band of 75kd in endothelial Cells but not smooth muscle Cells, fibroblasts, or B Cells. Cancer Cell lines PC3, DU145, MCF7, and K562 were also positive. Adipose-derived Stem Cells (ADSCs) expressed higher levels of Stro-1 when cultured beyond the first passage or when induced to differentiate into endothelial Cells. These data, together with previous studies, indicate that Stro-1 is intrinsically an endothelial antigen, and its expression in MSC is probably an induced event.
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Tissue distribution of mesenchymal Stem Cell Marker Stro-1.
Stem cells and development, 2011Co-Authors: Guiting Lin, Gang Liu, Lia Banie, Guifang Wang, Hongxiu Ning, Tom F. Lue, Ching-shwun LinAbstract:Stro-1 is the best-known mesenchymal Stem Cell Marker. However, despite its bone marrow origin, its localization in bone marrow has never been demonstrated. By immunofluorescence staining, we show here that ∼0.74% of nucleated bone morrow Cells expressed Stro-1. We also found that ∼8.7% of CD34-expressing Cells expressed Stro-1, and more than 20% of Stro-1-expressing Cells did not express CD34. In adipose tissue Stro-1 expression was identified in the endothelium of arterioles and capillaries. Stro-1 was also localized in the endothelium of some but not all adipose tissue veins. Endothelial expression of Stro-1 was also identified in blood vessels in penis and in leg muscles, but not in other tested tissues. In these other tissues, Stro-1 was scantly expressed near but not in blood vessels. These variable and endothelial expression patterns of Stro-1 point to a need to re-examine published data that relied on Stro-1 as a mesenchymal Stem Cell Marker.