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Andre Lipski - One of the best experts on this subject based on the ideXlab platform.
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Stenotrophomonas lactitubi sp nov and Stenotrophomonas indicatrix sp nov isolated from surfaces with food contact
International Journal of Systematic and Evolutionary Microbiology, 2018Co-Authors: Mareike Weber, Wiebke Schunemann, Peter Kampfer, Andre LipskiAbstract:Five Gram-stain-negative, rod-shaped, none-spore-forming isolates were obtained from biofilms on different sites of a milking machine in Germany. Another strain with similar morphological characteristics was isolated from dirty dishes. Based on phylogenetic analysis of the 16S rRNA and gyrB genes, all isolates were assigned to the genus Stenotrophomonas , but were divided into three different groups. Chemotaxonomic characterization of the isolates led to the detection of iso-C15 : 0 and anteiso-C15 : 0 as the predominant cellular fatty acids, as well as small amounts of the hydroxyl fatty acids iso-C11 : 0 3-OH, C12 : 0 3-OH and iso-C13 : 0 3-OH. One group could be assigned to the species Stenotrophomonas maltophilia , while the genome sequences of two groups displayed average nucleotide identity values of less than 94 % between each other and the genome sequences of the next related type strains Stenotrophomonas maltophilia ATCC 13637T and Stenotrophomonas rhizophila DSM 14405T. Further phylogenetic, phenotypic and chemotaxonomic analyses enabled the differentiation of these strains from these closely related species. They are therefore considered to represent two novel species, for which the names Stenotrophomonas lactitubi and Stenotrophomonas indicatrix are proposed, with strains M15T (=DSM 104152T=LMG29943T) and WS40T (=DSM28278T=LMG29942T) as type strains.
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characterization of n2o producing xanthomonas like isolates from biofilters as Stenotrophomonas nitritireducens sp nov luteimonas mephitis gen nov sp nov and pseudoxanthomonas broegbernensis gen nov sp nov
International Journal of Systematic and Evolutionary Microbiology, 2000Co-Authors: W Finkmann, Karlheinz Altendorf, Erko Stackebrandt, Andre LipskiAbstract:A group of yellow-pigmented isolates from ammonia-supplied biofilters showed an unusual denitrification reaction. All strains reduced nitrite but not nitrate without production of nitrogen (N2). The only product found was nitrous oxide (N2O). The strains were divided into two clusters and one separate strain by their fatty acid profiles, which were similar to the fatty acid profiles of the genera Xanthomonas and Stenotrophomonas. Analyses of the 165 rDNA sequences showed that these clusters and the separate strain form three independent lines within the Xanthomonas branch of the Proteobacteria. The evolutionary distances of the isolates to members of the related genera Xanthomonas, Stenotrophomonas and Xylella calculated by the 16S rDNA sequences led to the proposal of two new genera and three new species, Stenotrophomonas nitritireducens sp. nov., Luteimonas mephitis gen. nov., sp. nov. and Pseudoxanthomonas broegbernensis gen. nov., sp. nov. The type strains are Stenotrophomonas nitritireducens L2T (= DSM 12575T), Luteimonas mephitis B1953/27.1T (= DSM 12574T) and Pseudoxanthomonas broegbernensis B1616/1T (= DSM 12573T).
Jose L Martinez - One of the best experts on this subject based on the ideXlab platform.
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whole genome sequence of Stenotrophomonas maltophilia d457 a clinical isolate and a model strain
Journal of Bacteriology, 2012Co-Authors: Felipe Lira, Maria Blanca Sanchez, Jose L Martinez, Alvaro Hernandez, Eugeni Belda, Andres Moya, Francisco J SilvaAbstract:Stenotrophomonas maltophilia is an opportunistic pathogen with an environmental origin, and it is an increasingly relevant cause of nosocomial infections. Here we present the whole-genome sequence of S. maltophilia strain D457, a clinical isolate that is being used as a model for studying antibiotic resistance in this bacterial species.
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Stenotrophomonas maltophilia drug resistance
Future Microbiology, 2009Co-Authors: Maria Blanca Sanchez, Alvaro Hernandez, Jose L MartinezAbstract:Stenotrophomonas maltophilia has emerged in recent years as a paradigm of an intrinsically resistant, opportunistic bacterial pathogen with an environmental origin. The recent publication of the sequences of two S. maltophilia genomes has shown that this bacterium contains a large repertoire of antibiotic resistance determinants, probably contributing to its characteristic susceptibility to antibiotics. Among those determinants, the best characterized are a number of multidrug efflux pumps, beta-lactamases and aminoglycoside-inactivating enzymes. Recently, the presence of a gene coding for a Qnr determinant in the genome of S. maltophilia has also been described. Together, these elements confer resistance to several of the drugs currently used for treating infections. Besides these chromosomally encoded determinants, which evolved in S. maltophilia long before the recent human use of antibiotics, this bacterial species is acquiring novel resistance genes by horizontal gene transfer, thereby increasing its resistance. Future studies are required to fully understand the mechanisms of resistance, their regulation and potential crosstalk with S. maltophilia virulence, as well as the population dynamics of the different isolates of this bacterial species.
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the biocide triclosan selects Stenotrophomonas maltophilia mutants that overproduce the smedef multidrug efflux pump
Antimicrobial Agents and Chemotherapy, 2005Co-Authors: Patricia Sanchez, Eduardo Moreno, Jose L MartinezAbstract:The possibility that triclosan selects Stenotrophomonas maltophilia mutants overexpressing the multidrug resistance pump SmeDEF is analyzed. Five out of 12 triclosan-selected mutants were less susceptible to antibiotics than the wild-type strain and overproduced SmeDEF. Results are discussed in relation to current debates on the potential selection of antibiotic-resistant bacteria by household biocides.
Maria Blanca Sanchez - One of the best experts on this subject based on the ideXlab platform.
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antibiotic resistance in the opportunistic pathogen Stenotrophomonas maltophilia
Frontiers in Microbiology, 2015Co-Authors: Maria Blanca SanchezAbstract:Stenotrophomonas maltophilia is an environmental bacterium found in the soil, associated with plants and animals, and in aquatic environments. It is also an opportunistic pathogen now causing an increasing number of nosocomial infections. The treatment of S. maltophilia is quite difficult given its intrinsic resistance to a number of antibiotics, and because it is able to acquire new resistances via horizontal gene transfer and mutations. Certainly, strains resistant to quinolones, cotrimoxale and/or cephalosporins - antibiotics commonly used to treat S. maltophilia infections - have emerged. The increasing number of available S. maltophilia genomes has allowed the identification and annotation of a large number of antimicrobial and heavy metal resistance genes. Most encode inactivating enzymes and efflux pumps, but information on their role in intrinsic and acquired resistance is limited. Non-typical antibiotic resistance mechanisms that also form part of the intrinsic resistome have been identified via mutant library screening. These include non-typical antibiotic resistance genes, such as bacterial metabolism genes, and non-inheritable resistant phenotypes, such as biofilm formation and persistence. Their relationships with resistance are complex and require further study.
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whole genome sequence of Stenotrophomonas maltophilia d457 a clinical isolate and a model strain
Journal of Bacteriology, 2012Co-Authors: Felipe Lira, Maria Blanca Sanchez, Jose L Martinez, Alvaro Hernandez, Eugeni Belda, Andres Moya, Francisco J SilvaAbstract:Stenotrophomonas maltophilia is an opportunistic pathogen with an environmental origin, and it is an increasingly relevant cause of nosocomial infections. Here we present the whole-genome sequence of S. maltophilia strain D457, a clinical isolate that is being used as a model for studying antibiotic resistance in this bacterial species.
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Stenotrophomonas maltophilia drug resistance
Future Microbiology, 2009Co-Authors: Maria Blanca Sanchez, Alvaro Hernandez, Jose L MartinezAbstract:Stenotrophomonas maltophilia has emerged in recent years as a paradigm of an intrinsically resistant, opportunistic bacterial pathogen with an environmental origin. The recent publication of the sequences of two S. maltophilia genomes has shown that this bacterium contains a large repertoire of antibiotic resistance determinants, probably contributing to its characteristic susceptibility to antibiotics. Among those determinants, the best characterized are a number of multidrug efflux pumps, beta-lactamases and aminoglycoside-inactivating enzymes. Recently, the presence of a gene coding for a Qnr determinant in the genome of S. maltophilia has also been described. Together, these elements confer resistance to several of the drugs currently used for treating infections. Besides these chromosomally encoded determinants, which evolved in S. maltophilia long before the recent human use of antibiotics, this bacterial species is acquiring novel resistance genes by horizontal gene transfer, thereby increasing its resistance. Future studies are required to fully understand the mechanisms of resistance, their regulation and potential crosstalk with S. maltophilia virulence, as well as the population dynamics of the different isolates of this bacterial species.
Gabriele Berg - One of the best experts on this subject based on the ideXlab platform.
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root microbe systems the effect and mode of interaction of stress protecting agent spa Stenotrophomonas rhizophila dsm14405t
Frontiers in Plant Science, 2013Co-Authors: Peyman Alavi, Margaret R Starcher, Christin Zachow, Henry Muller, Gabriele BergAbstract:Stenotrophomonas rhizophila has great potential for applications in biotechnology and biological control due to its ability to both promote plant growth and protect roots against biotic and a-biotic stresses, yet little is known about the mode of interactions in the root-environment system. We studied mechanisms associated with osmotic stress using transcriptomic and microscopic approaches. In response to salt and root extracts, the transcriptome of S. rhizophila DSM14405T changed drastically. We found a notably similar response for several functional gene groups responsible for general stress protection, energy production, and cell motility. However, unique changes in the transcriptome were also observed: the negative regulation of flagella-coding genes together with the up-regulation of the genes responsible for biofilm formation and alginate biosynthesis were identified as a single mechanism of S. rhizophila DSM14405T against salt shock. However, production and excretion of glucosylglycerol (GG) were found as a remarkable mechanism for the stress protection of this Stenotrophomonas strain. For S. rhizophila treated with root exudates, the shift from the planktonic lifestyle to a sessile one was measured as expressed in the down-regulation of flagellar-driven motility. These findings fit well with the observed positive regulation of host colonization genes and microscopic images that show different colonization patterns of oilseed rape roots. Spermidine, described as a plant growth regulator, was also newly identified as a protector against stress. Overall, we identified mechanisms of Stenotrophomonas to protect roots against osmotic stress in the environment. In addition to both the changes in life style and energy metabolism, phytohormons and osmoprotectants were also found to play a key role in stress protection.
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the versatility and adaptation of bacteria from the genus Stenotrophomonas
Nature Reviews Microbiology, 2009Co-Authors: Robert P Ryan, Lisa Crossman, Matthew B Avison, Sebastien Monchy, Massimiliano Cardinale, Safiyh Taghavi, Gabriele Berg, Daniel Van Der Lelie, Maxwell J DowAbstract:The genus Stenotrophomonas comprises at least eight species. These bacteria are found throughout the environment, particularly in close association with plants. Strains of the most predominant species, Stenotrophomonas maltophilia, have an extraordinary range of activities that include beneficial effects for plant growth and health, the breakdown of natural and man-made pollutants that are central to bioremediation and phytoremediation strategies and the production of biomolecules of economic value, as well as detrimental effects, such as multidrug resistance, in human pathogenic strains. Here, we discuss the versatility of the bacteria in the genus Stenotrophomonas and the insight that comparative genomic analysis of clinical and endophytic isolates of S. maltophilia has brought to our understanding of the adaptation of this genus to various niches.
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the rhizosphere as a reservoir for opportunistic human pathogenic bacteria
Environmental Microbiology, 2005Co-Authors: Gabriele Berg, Leo Eberl, Anton HartmannAbstract:Summary During the last years, the number of human infections caused by opportunistic pathogens has increased dramatically. One natural reservoir of opportunistic pathogens is the rhizosphere, the zone around roots that is influenced by the plant. Due to a high content of nutrients, this habitat is a ‘microbial hot-spot’, where bacterial abundances including those with strong antagonistic traits are enhanced. Various bacterial genera, including Burkholderia , Enterobacter , Herbaspirillum , Ochrobactrum , Pseudomonas , Ralstonia , Staphylococcus and Stenotrophomonas , contain root-associated strains that can encounter bivalent interactions with both plant and human hosts. Mechanisms responsible for colonization of the rhizosphere and antagonistic activity against plant pathogens are similar to those responsible for colonization of human organs and tissues, and pathogenicity. Multiple resistances against antibiotics are not only found with clinical strains but also with strains isolated from the rhizosphere. High competition, the occurrence of diverse antibiotics in the rhizosphere, and enhanced horizontal gene transfer rates in this microenvironment appear to contribute to the high levels of natural resistances. While opportunistic bacteria from the rhizosphere have some properties in common, each of these emerging pathogens has its own features, which are discussed in detail for Burkholderia , Ochrobactrum and Stenotrophomonas .
Guanlin Xie - One of the best experts on this subject based on the ideXlab platform.
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genome sequence of Stenotrophomonas maltophilia rr 10 isolated as an endophyte from rice root
Journal of Bacteriology, 2012Co-Authors: Bo Zhu, He Liu, Wenxiao Tian, Xiaoying Fan, Xueping Zhou, Gulei Jin, Guanlin XieAbstract:Stenotrophomonas maltophilia is an endophyte which plays important roles in agricultural production as a plant growth-promoting bacterium. Here, we present the draft genome sequence of strain RR-10, which was isolated from a rice root in a rice field of China.