The Experts below are selected from a list of 276 Experts worldwide ranked by ideXlab platform
Xinxin Zhang - One of the best experts on this subject based on the ideXlab platform.
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new Steroid Saponins from dioscorea zingiberensis yam and their medicinal use against i r via anti inflammatory effect
Food & Function, 2021Co-Authors: Zhaowei Xue, Xinxin Zhang, Ming Jin, Yoichiro Ito, Zhen Cao, Xinrui Wang, Jianwei Dou, Yuhong Zhu, Zengjun GuoAbstract:Steroid Saponins are the medicinal compounds and nutrition ingredients of medicine food homology (MFH) Dioscorea zingiberensis C. H. Wright (D. zingiberensis) yam. Our phytochemical investigation of the edible rhizomes resulted in 9 new furostanol Steroid Saponins named dioscins A-I (1-9), together with 11 known Steroid Saponins. Their chemical structures were elucidated based on spectroscopic and chemical analyses. The new dioscins were evaluated for their anti-inflammatory and beneficial effects against cerebral ischemia reperfusion (I/R) injury on RAW264.7 and PC12 cells in vitro, respectively. Dioscins A, B, and G revealed considerable anti-I/R effect through an anti-inflammatory mechanism based on the decreasing concentration of pro-inflammatory (TNF-α and IL-6) and down-regulating the NF-κB expression. The present research demonstrated that daily consumption of this yam plant probably prevented the I/R occurrence via the anti-inflammatory property of Steroid Saponins, and it also enriched the Steroid saponin library, providing the possibility to develop MFH-containing Steroid Saponins into functional foods for maintenance of human health or drugs for the treatment of I/R disease.
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safety investigation on total Steroid Saponins extracts from dioscorea zingiberensis c h wright sub acute and chronic toxicity studies on dogs
Regulatory Toxicology and Pharmacology, 2017Co-Authors: Xinxin Zhang, Hui Zhang, Ming Jin, Nigatu Tadesse, Liang Xian, Sicen Wang, Jun Dang, Yan Zhang, Zengjun Guo, Yoichiro ItoAbstract:Sub-acute and chronic toxic effects of total Steroidal Saponins (TSSN) extracts from Dioscorea zingiberensis C.H. Wright on various internal organs and biochemical indicators have never been studied before and this study is the first of its kind to demonstrate sub-acute and chronic toxicities of TSSN on dogs. Administration of TSSN extracts at doses up to 3000 mg/Kg daily for 14 days, no biochemical and organ changes were observed on the experimental groups of dogs. Further, chronic toxicity study through oral administration of TSSN extracts at the gradual doses of 50, 250 and 500 mg/Kg for 90 days followed by a 2-week recovery assay revealed absence of significant architectural and morphological changes in internal organs which were confirmed through histopathological examination and merely no significant alteration in the biochemical indicators including hematologic and urine analysis and electrocardiogram compared to the control dogs. This toxicological evaluation came across with the finding that the herbal preparation can be considered as nontoxic and animals could tolerate the extracts at doses up to 500 mg/Kg with LD50 greater than 3000 mg/Kg. It may serve as a preliminary scientific evidence for further therapeutic investigations.
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comparative studies on performance of ccc and preparative rp hplc in separation and purification of Steroid Saponins from dioscorea zingiberensis c h wright
Journal of Steroids & Hormonal Science, 2015Co-Authors: Xinxin Zhang, Jinru Liang, Wenji Sun, Jianli Liu, Yongmin Zhang, Yoichiro ItoAbstract:SteroidSaponins from Dioscorea zingiberensis C.H.Wright were separated for the first time using two chromatographic methods for comparison: counter-current chromatography (CCC) coupled with evaporative light scattering detector (ELSD) and preparative reversed phase high-performance liquid chromatography (RP-HPLC) with an ultraviolet detector. Ethyl acetate-n-butanol-methanol-water (4:1:2:4, v/v) was chosen as the two-phase solvent system for CCC, while the acetonitrile-water (25:75 for the first step and15:85 for the second step, v/v) was used as the mobile phase in the preparative RP-HPLC. The following five Steroid Saponins were purified by these two chromatographic methods, in one-step operation by CCC and by two-step operation in preparative RP-HPLC:
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simultaneous quantification of five Steroid Saponins from dioscorea zingiberensis c h wright in rat plasma by hplc ms ms and its application to the pharmacokinetic studies
Steroids, 2015Co-Authors: Xinxin Zhang, Yoichiro Ito, Wenji SunAbstract:A simple, reliable and sensitive high-performance liquid chromatography tandem mass spectrometry method (HPLC-MS/MS) was established for simultaneous analyses of the following 5 Steroid Saponins in rat plasma after the single dose administration of total Steroid Saponins extracted from the rhizome of Dioscorea zingiberensis C.H. Wright for the first time. Protodioscin, huangjiangsu A, zingiberensis new saponin, dioscin, and gracillin were quantified using ginsenoside Rb1 as the internal standard (IS). The plasma samples were pretreated by a single step acetonitrile-mediated protein precipitation. The chromatographic separation was performed on an Inersil ODS-3 C18 column (250mm×4.6mm, 5μm) with the mobile phase composed of acetonitrile and water containing 0.1% formic acid under a gradient elution mode at 0.2mLmin(-1) using a microsplit after the eluent from the HPLC apparatus. The quantification was accomplished on a triple quadrupole tandem mass spectrometer using the multiple reaction monitoring (MRM) in the positive ionization mode. The above five analytes were stable under sample storage and preparation conditions applied in the present study. The linearity, precision, accuracy, and recoveries of the analysis confirmed the requirements for quality-control purposes. After validation, this proposed method was successfully adopted to investigate the pharmacokinetic parameters of these five analytes.
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therapeutic effects of total Steroid saponin extracts from the rhizome of dioscorea zingiberensis c h wright in freund s complete adjuvant induced arthritis in rats
International Immunopharmacology, 2014Co-Authors: Xinxin Zhang, Yoichiro Ito, Jinru Liang, Jianli Liu, Wenji SunAbstract:The aim of our present study is to explore the anti-arthritic potential effect of total Steroid Saponins (TSSNs) extracted from the rhizome of Dioscorea zingiberensis C.H.Wright (DZW) and to investigate the underlying mechanisms. This work was performed using adjuvant-induced arthritis (AIA) rats in vivo and lipopolysaccharide (LPS) simulated 264.7 macrophage cells in vitro. In AIA-induced arthritic rats, TSSN significantly alleviated the arthritic progression through evaluating arthritic score, immune organ indexes, paw swelling, and body weight. This phenomenon was well correlated with significant suppression of the overproduction of inflammation cytokines (IL-1, IL-1β, IL-6, and TNF-α), oxidant stress makers (MDA and NO), eicosanoids (LTB4 and PGE2), and inflammatory enzymes (5-LOX and COX-2) versus the AIA rats without treatment. On the contrary, the release of SOD and IL-10 was profoundly increased. What's more, TSSN could obviously ameliorate the translocation of NF-κB to the nucleus through phosphorylation of the p65 and IκBα in vivo and in vitro. The current findings demonstrated that TSSN could protect the injured ankle joint from further deterioration and exert its satisfactory anti-arthritis properties through anti-inflammatory and anti-oxidant effects via inactivating the NF-κB signal pathway. This research implies that DZW may be a useful therapeutic agent for the treatment of human arthritis.
Wenji Sun - One of the best experts on this subject based on the ideXlab platform.
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comparative studies on performance of ccc and preparative rp hplc in separation and purification of Steroid Saponins from dioscorea zingiberensis c h wright
Journal of Steroids & Hormonal Science, 2015Co-Authors: Xinxin Zhang, Jinru Liang, Wenji Sun, Jianli Liu, Yongmin Zhang, Yoichiro ItoAbstract:SteroidSaponins from Dioscorea zingiberensis C.H.Wright were separated for the first time using two chromatographic methods for comparison: counter-current chromatography (CCC) coupled with evaporative light scattering detector (ELSD) and preparative reversed phase high-performance liquid chromatography (RP-HPLC) with an ultraviolet detector. Ethyl acetate-n-butanol-methanol-water (4:1:2:4, v/v) was chosen as the two-phase solvent system for CCC, while the acetonitrile-water (25:75 for the first step and15:85 for the second step, v/v) was used as the mobile phase in the preparative RP-HPLC. The following five Steroid Saponins were purified by these two chromatographic methods, in one-step operation by CCC and by two-step operation in preparative RP-HPLC:
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simultaneous quantification of five Steroid Saponins from dioscorea zingiberensis c h wright in rat plasma by hplc ms ms and its application to the pharmacokinetic studies
Steroids, 2015Co-Authors: Xinxin Zhang, Yoichiro Ito, Wenji SunAbstract:A simple, reliable and sensitive high-performance liquid chromatography tandem mass spectrometry method (HPLC-MS/MS) was established for simultaneous analyses of the following 5 Steroid Saponins in rat plasma after the single dose administration of total Steroid Saponins extracted from the rhizome of Dioscorea zingiberensis C.H. Wright for the first time. Protodioscin, huangjiangsu A, zingiberensis new saponin, dioscin, and gracillin were quantified using ginsenoside Rb1 as the internal standard (IS). The plasma samples were pretreated by a single step acetonitrile-mediated protein precipitation. The chromatographic separation was performed on an Inersil ODS-3 C18 column (250mm×4.6mm, 5μm) with the mobile phase composed of acetonitrile and water containing 0.1% formic acid under a gradient elution mode at 0.2mLmin(-1) using a microsplit after the eluent from the HPLC apparatus. The quantification was accomplished on a triple quadrupole tandem mass spectrometer using the multiple reaction monitoring (MRM) in the positive ionization mode. The above five analytes were stable under sample storage and preparation conditions applied in the present study. The linearity, precision, accuracy, and recoveries of the analysis confirmed the requirements for quality-control purposes. After validation, this proposed method was successfully adopted to investigate the pharmacokinetic parameters of these five analytes.
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therapeutic effects of total Steroid saponin extracts from the rhizome of dioscorea zingiberensis c h wright in freund s complete adjuvant induced arthritis in rats
International Immunopharmacology, 2014Co-Authors: Xinxin Zhang, Yoichiro Ito, Jinru Liang, Jianli Liu, Wenji SunAbstract:The aim of our present study is to explore the anti-arthritic potential effect of total Steroid Saponins (TSSNs) extracted from the rhizome of Dioscorea zingiberensis C.H.Wright (DZW) and to investigate the underlying mechanisms. This work was performed using adjuvant-induced arthritis (AIA) rats in vivo and lipopolysaccharide (LPS) simulated 264.7 macrophage cells in vitro. In AIA-induced arthritic rats, TSSN significantly alleviated the arthritic progression through evaluating arthritic score, immune organ indexes, paw swelling, and body weight. This phenomenon was well correlated with significant suppression of the overproduction of inflammation cytokines (IL-1, IL-1β, IL-6, and TNF-α), oxidant stress makers (MDA and NO), eicosanoids (LTB4 and PGE2), and inflammatory enzymes (5-LOX and COX-2) versus the AIA rats without treatment. On the contrary, the release of SOD and IL-10 was profoundly increased. What's more, TSSN could obviously ameliorate the translocation of NF-κB to the nucleus through phosphorylation of the p65 and IκBα in vivo and in vitro. The current findings demonstrated that TSSN could protect the injured ankle joint from further deterioration and exert its satisfactory anti-arthritis properties through anti-inflammatory and anti-oxidant effects via inactivating the NF-κB signal pathway. This research implies that DZW may be a useful therapeutic agent for the treatment of human arthritis.
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neuroprotection of total Steroid Saponins from dioscorea zingiberensis against transient focal cerebral ischemia reperfusion injury in rats via anti inflammatory and antiapoptotic effects
Planta Medica, 2014Co-Authors: Xinxin Zhang, Yoichiro Ito, Lin Chen, Jianli Liu, Wenji SunAbstract:Total Steroid Saponins isolated from Dioscorea zingiberensis have shown a variety of beneficial bioactivities. However, there are no reports about their neuroprotective effects, until now. Therefore, in the present study, we explored the neuroprotective effects of the total Steroid Saponins from D. zingiberensis on rats against transient focal cerebral ischemia-reperfusion and their underlying mechanisms. Healthy adult Sprague-Dawley rats were randomly assigned to six groups. After pretreatment with D. zingiberensis total Steroid Saponins intragastrically for six days, the rats were subjected to an ischemia injury by surgery on the middle cerebral artery occlusion for 90 min. Compared to the ischemia-reperfusion group, the D. zingiberensis total Steroid saponin group of rats, especially those given a 30-mg/kg dose, showed not only a marked reduction in neurological deficit scores, cerebral infarct volume, and brain edema, but also an increase in neuron survival (Nissl bodies) in the hippocampal cornuammons 1 and cortex hemisphere of the ipsilateral ischemia. At the same time, the inflammatory cytokines in serum induced by the middle cerebral artery occlusion were significantly decreased by the preadministration of D. zingiberensis total Steroid Saponins. Furthermore, the increase of caspase-3 was evidently reduced in the hippocampal cornuammons 1 and cortex of the hemisphere injured brain. Finally, the downregulating antiapoptotic Bcl-2 and upregulating proapoptotic Bax proteins were obviously suppressed. Taken together, the current findings suggest that D. zingiberensis total Steroid Saponins played a potential neuroprotective role against a severe injury induced by transient focal cerebral ischemic reperfusion in a rat experimental model, and this role may be mediated by its anti-inflammatory and antiapoptotic actions.
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neuroprotective effects of total Steroid Saponins on cerebral ischemia injuries in an animal model of focal ischemia reperfusion
Planta Medica, 2014Co-Authors: Xinxin Zhang, Yoichiro Ito, Lin Chen, Jianli Liu, Xuan Dang, Wenji SunAbstract:Total Steroid Saponins isolated from Dioscorea zingiberensis are a unique traditional Chinese medicine known for its potential usage in various types of diseases. However, there is little evidence about its neuroprotective effect in transient focal ischemia-reperfusion cerebral injury. Therefore, the current study was carried out to investigate the effect of total Steroid Saponins on neuroprotection and its potential mechanisms in the rat ischemia-reperfusion model by middle cerebral artery occlusion for 90 min. The rats were each treated with total Steroid Saponins (30 mg/kg, 10 mg/kg, and 3 mg/kg) or nimodipine (20 mg/kg) daily for 6 days before middle cerebral artery occlusion. Then, the neurological deficit score, cerebral infarct volume, and brain water content were measured at 24 h after reperfusion. Meanwhile, the histopathological changes and AQP-4 protein activities were examined in hippocampal CA1 and the cortex of ipsilateral ischemic cerebral hemisphere by hematoxylin-eosin staining and immunohistochemistry, respectively. The indices of oxidative stress in the serum were also obtained, and NF- κ B and ERK 1/2 protein expressions in the injured brain were evaluated by Western blotting. The results indicated that the pre-treatment with these drugs not only significantly reduced cerebral infarct volume, brain water content and improved neurological deficit score, but also restored neuronal morphology and decreased the AQP-4 positive cells in CA1 and the cortex. Moreover, it markedly restored the level of oxidant stress markers (CAT, SOD, MDA, NO and iNOS) to their normal range in serum. In addition, the increased NF- κ B and ERK 1/2 protein expressions were alleviated as compared with the ischemia-reperfusion group. These findings demonstrate that total Steroid Saponins exhibit promising neuroprotection effects against the transient focal ischemia-reperfusion cerebral injury in the rat experimental model and the underlying mechanisms might be mediated through inhibition of anti-edema as well as anti-oxidative effects by inactivation of NF- κ B and ERK 1/2 signalling pathway.
Lisheng Ding - One of the best experts on this subject based on the ideXlab platform.
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esi qqtof ms ms and apci it ms ms analysis of Steroid Saponins from the rhizomes of dioscorea panthaica
Journal of Mass Spectrometry, 2006Co-Authors: Yan Zhou, Lisheng DingAbstract:Using high-resolution quadrupole time-of-flight mass spectrometry along with an electrospray ionization source (ESI-QqTOF-MS), accurate molecular weights of 13 Steroid Saponins extracted from the rhizomes of Dioscorea panthaica were acquired and the corresponding molecular formulae obtained. In order to elucidate the fragmentation pathways of Steroid Saponins in D. panthaica, 10 authentic samples were investigated using ESI-QqTOF-MS/MS. In addition, atmospheric pressure chemical ionization mass spectrometry combined with ion trap tandem mass spectrometry (APCI-IT-MS/MS) was used to analyze the structures of 13 Steroid Saponins in D. panthaica. Through the analysis of their tandem mass data, diagnostic fragment ions of the spirostanol and furostanol Steroid Saponins in D. panthaica were detected as m/z 271.2056 and 253.1951. In addition, four pairs of isomers were detected and the possible structures of four unknown Steroid Saponins in D. panthaica speculated. ESI-TOF and APCI-MS(n) have proved to be effective tools for research on fragmentation mechanism of Steroid Saponins and the rapid determination of native Steroid Saponins in extract mixture, thereby avoiding tedious derivation and separation steps.
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esi qqtof ms ms and apci it ms ms analysis of Steroid Saponins from the rhizomes of dioscorea panthaica
Journal of Mass Spectrometry, 2006Co-Authors: Rui Li, Yan Zhou, Zhijun Wu, Lisheng DingAbstract:Using high-resolution quadrupole time-of-flight mass spectrometry along with an electrospray ionization source (ESI-QqTOF-MS), accurate molecular weights of 13 Steroid Saponins extracted from the rhizomes of Dioscorea panthaica were acquired and the corresponding molecular formulae obtained. In order to elucidate the fragmentation pathways of Steroid Saponins in D. panthaica, 10 authentic samples were investigated using ESI-QqTOF-MS/MS. In addition, atmospheric pressure chemical ionization mass spectrometry combined with ion trap tandem mass spectrometry (APCI-IT-MS/MS) was used to analyze the structures of 13 Steroid Saponins in D. panthaica. Through the analysis of their tandem mass data, diagnostic fragment ions of the spirostanol and furostanol Steroid Saponins in D. panthaica were detected as m/z 271.2056 and 253.1951. In addition, four pairs of isomers were detected and the possible structures of four unknown Steroid Saponins in D. panthaica speculated. ESI-TOF and APCI-MSn have proved to be effective tools for research on fragmentation mechanism of Steroid Saponins and the rapid determination of native Steroid Saponins in extract mixture, thereby avoiding tedious derivation and separation steps. Copyright (c) 2006 John Wiley & Sons, Ltd.
Yoichiro Ito - One of the best experts on this subject based on the ideXlab platform.
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new Steroid Saponins from dioscorea zingiberensis yam and their medicinal use against i r via anti inflammatory effect
Food & Function, 2021Co-Authors: Zhaowei Xue, Xinxin Zhang, Ming Jin, Yoichiro Ito, Zhen Cao, Xinrui Wang, Jianwei Dou, Yuhong Zhu, Zengjun GuoAbstract:Steroid Saponins are the medicinal compounds and nutrition ingredients of medicine food homology (MFH) Dioscorea zingiberensis C. H. Wright (D. zingiberensis) yam. Our phytochemical investigation of the edible rhizomes resulted in 9 new furostanol Steroid Saponins named dioscins A-I (1-9), together with 11 known Steroid Saponins. Their chemical structures were elucidated based on spectroscopic and chemical analyses. The new dioscins were evaluated for their anti-inflammatory and beneficial effects against cerebral ischemia reperfusion (I/R) injury on RAW264.7 and PC12 cells in vitro, respectively. Dioscins A, B, and G revealed considerable anti-I/R effect through an anti-inflammatory mechanism based on the decreasing concentration of pro-inflammatory (TNF-α and IL-6) and down-regulating the NF-κB expression. The present research demonstrated that daily consumption of this yam plant probably prevented the I/R occurrence via the anti-inflammatory property of Steroid Saponins, and it also enriched the Steroid saponin library, providing the possibility to develop MFH-containing Steroid Saponins into functional foods for maintenance of human health or drugs for the treatment of I/R disease.
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safety investigation on total Steroid Saponins extracts from dioscorea zingiberensis c h wright sub acute and chronic toxicity studies on dogs
Regulatory Toxicology and Pharmacology, 2017Co-Authors: Xinxin Zhang, Hui Zhang, Ming Jin, Nigatu Tadesse, Liang Xian, Sicen Wang, Jun Dang, Yan Zhang, Zengjun Guo, Yoichiro ItoAbstract:Sub-acute and chronic toxic effects of total Steroidal Saponins (TSSN) extracts from Dioscorea zingiberensis C.H. Wright on various internal organs and biochemical indicators have never been studied before and this study is the first of its kind to demonstrate sub-acute and chronic toxicities of TSSN on dogs. Administration of TSSN extracts at doses up to 3000 mg/Kg daily for 14 days, no biochemical and organ changes were observed on the experimental groups of dogs. Further, chronic toxicity study through oral administration of TSSN extracts at the gradual doses of 50, 250 and 500 mg/Kg for 90 days followed by a 2-week recovery assay revealed absence of significant architectural and morphological changes in internal organs which were confirmed through histopathological examination and merely no significant alteration in the biochemical indicators including hematologic and urine analysis and electrocardiogram compared to the control dogs. This toxicological evaluation came across with the finding that the herbal preparation can be considered as nontoxic and animals could tolerate the extracts at doses up to 500 mg/Kg with LD50 greater than 3000 mg/Kg. It may serve as a preliminary scientific evidence for further therapeutic investigations.
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comparative studies on performance of ccc and preparative rp hplc in separation and purification of Steroid Saponins from dioscorea zingiberensis c h wright
Journal of Steroids & Hormonal Science, 2015Co-Authors: Xinxin Zhang, Jinru Liang, Wenji Sun, Jianli Liu, Yongmin Zhang, Yoichiro ItoAbstract:SteroidSaponins from Dioscorea zingiberensis C.H.Wright were separated for the first time using two chromatographic methods for comparison: counter-current chromatography (CCC) coupled with evaporative light scattering detector (ELSD) and preparative reversed phase high-performance liquid chromatography (RP-HPLC) with an ultraviolet detector. Ethyl acetate-n-butanol-methanol-water (4:1:2:4, v/v) was chosen as the two-phase solvent system for CCC, while the acetonitrile-water (25:75 for the first step and15:85 for the second step, v/v) was used as the mobile phase in the preparative RP-HPLC. The following five Steroid Saponins were purified by these two chromatographic methods, in one-step operation by CCC and by two-step operation in preparative RP-HPLC:
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simultaneous quantification of five Steroid Saponins from dioscorea zingiberensis c h wright in rat plasma by hplc ms ms and its application to the pharmacokinetic studies
Steroids, 2015Co-Authors: Xinxin Zhang, Yoichiro Ito, Wenji SunAbstract:A simple, reliable and sensitive high-performance liquid chromatography tandem mass spectrometry method (HPLC-MS/MS) was established for simultaneous analyses of the following 5 Steroid Saponins in rat plasma after the single dose administration of total Steroid Saponins extracted from the rhizome of Dioscorea zingiberensis C.H. Wright for the first time. Protodioscin, huangjiangsu A, zingiberensis new saponin, dioscin, and gracillin were quantified using ginsenoside Rb1 as the internal standard (IS). The plasma samples were pretreated by a single step acetonitrile-mediated protein precipitation. The chromatographic separation was performed on an Inersil ODS-3 C18 column (250mm×4.6mm, 5μm) with the mobile phase composed of acetonitrile and water containing 0.1% formic acid under a gradient elution mode at 0.2mLmin(-1) using a microsplit after the eluent from the HPLC apparatus. The quantification was accomplished on a triple quadrupole tandem mass spectrometer using the multiple reaction monitoring (MRM) in the positive ionization mode. The above five analytes were stable under sample storage and preparation conditions applied in the present study. The linearity, precision, accuracy, and recoveries of the analysis confirmed the requirements for quality-control purposes. After validation, this proposed method was successfully adopted to investigate the pharmacokinetic parameters of these five analytes.
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therapeutic effects of total Steroid saponin extracts from the rhizome of dioscorea zingiberensis c h wright in freund s complete adjuvant induced arthritis in rats
International Immunopharmacology, 2014Co-Authors: Xinxin Zhang, Yoichiro Ito, Jinru Liang, Jianli Liu, Wenji SunAbstract:The aim of our present study is to explore the anti-arthritic potential effect of total Steroid Saponins (TSSNs) extracted from the rhizome of Dioscorea zingiberensis C.H.Wright (DZW) and to investigate the underlying mechanisms. This work was performed using adjuvant-induced arthritis (AIA) rats in vivo and lipopolysaccharide (LPS) simulated 264.7 macrophage cells in vitro. In AIA-induced arthritic rats, TSSN significantly alleviated the arthritic progression through evaluating arthritic score, immune organ indexes, paw swelling, and body weight. This phenomenon was well correlated with significant suppression of the overproduction of inflammation cytokines (IL-1, IL-1β, IL-6, and TNF-α), oxidant stress makers (MDA and NO), eicosanoids (LTB4 and PGE2), and inflammatory enzymes (5-LOX and COX-2) versus the AIA rats without treatment. On the contrary, the release of SOD and IL-10 was profoundly increased. What's more, TSSN could obviously ameliorate the translocation of NF-κB to the nucleus through phosphorylation of the p65 and IκBα in vivo and in vitro. The current findings demonstrated that TSSN could protect the injured ankle joint from further deterioration and exert its satisfactory anti-arthritis properties through anti-inflammatory and anti-oxidant effects via inactivating the NF-κB signal pathway. This research implies that DZW may be a useful therapeutic agent for the treatment of human arthritis.
Jinru Liang - One of the best experts on this subject based on the ideXlab platform.
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comparative studies on performance of ccc and preparative rp hplc in separation and purification of Steroid Saponins from dioscorea zingiberensis c h wright
Journal of Steroids & Hormonal Science, 2015Co-Authors: Xinxin Zhang, Jinru Liang, Wenji Sun, Jianli Liu, Yongmin Zhang, Yoichiro ItoAbstract:SteroidSaponins from Dioscorea zingiberensis C.H.Wright were separated for the first time using two chromatographic methods for comparison: counter-current chromatography (CCC) coupled with evaporative light scattering detector (ELSD) and preparative reversed phase high-performance liquid chromatography (RP-HPLC) with an ultraviolet detector. Ethyl acetate-n-butanol-methanol-water (4:1:2:4, v/v) was chosen as the two-phase solvent system for CCC, while the acetonitrile-water (25:75 for the first step and15:85 for the second step, v/v) was used as the mobile phase in the preparative RP-HPLC. The following five Steroid Saponins were purified by these two chromatographic methods, in one-step operation by CCC and by two-step operation in preparative RP-HPLC:
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therapeutic effects of total Steroid saponin extracts from the rhizome of dioscorea zingiberensis c h wright in freund s complete adjuvant induced arthritis in rats
International Immunopharmacology, 2014Co-Authors: Xinxin Zhang, Yoichiro Ito, Jinru Liang, Jianli Liu, Wenji SunAbstract:The aim of our present study is to explore the anti-arthritic potential effect of total Steroid Saponins (TSSNs) extracted from the rhizome of Dioscorea zingiberensis C.H.Wright (DZW) and to investigate the underlying mechanisms. This work was performed using adjuvant-induced arthritis (AIA) rats in vivo and lipopolysaccharide (LPS) simulated 264.7 macrophage cells in vitro. In AIA-induced arthritic rats, TSSN significantly alleviated the arthritic progression through evaluating arthritic score, immune organ indexes, paw swelling, and body weight. This phenomenon was well correlated with significant suppression of the overproduction of inflammation cytokines (IL-1, IL-1β, IL-6, and TNF-α), oxidant stress makers (MDA and NO), eicosanoids (LTB4 and PGE2), and inflammatory enzymes (5-LOX and COX-2) versus the AIA rats without treatment. On the contrary, the release of SOD and IL-10 was profoundly increased. What's more, TSSN could obviously ameliorate the translocation of NF-κB to the nucleus through phosphorylation of the p65 and IκBα in vivo and in vitro. The current findings demonstrated that TSSN could protect the injured ankle joint from further deterioration and exert its satisfactory anti-arthritis properties through anti-inflammatory and anti-oxidant effects via inactivating the NF-κB signal pathway. This research implies that DZW may be a useful therapeutic agent for the treatment of human arthritis.
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therapeutic effects of total Steroid saponin extracts from the rhizome of dioscorea zingiberensis c h wright in freund s complete adjuvant induced arthritis in rats
International Immunopharmacology, 2014Co-Authors: Xinxin Zhang, Jinru Liang, Jiao HeAbstract:The aim of our present study is to explore the anti-arthritic potential effect of total Steroid Saponins (TSSNs) extracted from the rhizome of Dioscorea zingiberensis C.H.Wright (DZW) and to investigate the underlying mechanisms. This work was performed using adjuvant-induced arthritis (AIA) rats in vivo and lipopolysaccharide (LPS) simulated 264.7 macrophage cells in vitro. In AIA-induced arthritic rats, TSSN significantly alleviated the arthritic progression through evaluating arthritic score, immune organ indexes, paw swelling, and body weight. This phenomenon was well correlated with significant suppression of the overproduction of inflammation cytokines (IL-1, IL-1β, IL-6, and TNF-α), oxidant stress makers (MDA and NO), eicosanoids (LTB4 and PGE2), and inflammatory enzymes (5-LOX and COX-2) versus the AIA rats without treatment. On the contrary, the release of SOD and IL-10 was profoundly increased. What's more, TSSN could obviously ameliorate the translocation of NF-κB to the nucleus through phosphorylation of the p65 and IκBα in vivo and in vitro. The current findings demonstrated that TSSN could protect the injured ankle joint from further deterioration and exert its satisfactory anti-arthritis properties through anti-inflammatory and anti-oxidant effects via inactivating the NF-κB signal pathway. This research implies that DZW may be a useful therapeutic agent for the treatment of human arthritis.
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quality control and identification of Steroid Saponins from dioscorea zingiberensis c h wright by fingerprint with hplc elsd and hplc esi quadrupole time of fight tandem mass spectrometry
Journal of Pharmaceutical and Biomedical Analysis, 2014Co-Authors: Xinxin Zhang, Jinru Liang, Ye ZhaoAbstract:Abstract In this study, a fingerprint of Steroid Saponins, the major bioactive constituents in the crude extracts from Dioscorea zingiberensis C. H. Wright (DZW), has been established for the first time by combined use of the following two methods: high-performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD) and the simultaneous characterization of the Steroid Saponins by high-performance liquid chromatography coupled with electrospray ionization-mass spectrometry and quadrupole tandem time-of-fight mass analyzers detection (HPLC-ESI-Q/TOF). All HPLC analyses were carried out on a Welchrom C 18 column (250 mm × 4.6 mm I.D., 5 μm) with a mobile phase composed of water and acetonitrile under gradient elution. There were 68 common characteristic peaks in the fingerprints, in which 12 of them were confirmed by comparing their mass spectra and retention times with those of the reference compounds. In order to identify other unknown peaks, their fragmentation behaviors characteristic of the major groups of Steroid Saponins from DZW with six types of aglycone skeletons were discussed in detail, and possible MS/MS fragmentation pathways were proposed for aiding the structural identification of these components. According to the summarized fragmentation patterns, these peaks were tentatively assigned by matching their empirical molecular formula with those of the published compounds, or by elucidating their quasi-molecular ions and fragment ions referring to available literature information when the reference standards were unavailable. As a result, 22 new Steroid Saponins were found in DZW for the first time. In addition, the quantitative analysis of the nine (except for the reference compounds A , B , and C ) known peaks was accomplished at the same time which indicated that there was a great variability in the amount of these active compounds in different batches in the crude extracts. This approach could demonstrate that the fingerprint could be considered to be a suitable tool to comprehensively improve the quality control of DZW. The identification and structural elucidation of the peaks in the fingerprint may provide important experimental data for further pharmacological and clinical researches.
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study on hplc elsd fingerprint of total Steroid Saponins in herbs of dioscorea zingiberensis
China journal of Chinese materia medica, 2013Co-Authors: Xinxin Zhang, Jinru Liang, Ye Zhao, Wenji SunAbstract:To establish a HPLC-ELSD fingerprint for total Steroid Saponins in herbs of Dioscorea zingiberensis. Welchrom C,8 (4. 6 mm x 250 mm,5 microm) chromatographic column was adopted and eluted with the mobile phase of acetonitrile (A)-water (B) at the flow rate of 1.0 mL min-1. The column temperature was room temperature. The ELSD conditions were as follows: the temperature of drift tube was 90.0 degreeC, the flow rate of carrier gas (N2) was 2. 8 L min-1, and the injection volume was 10 microL. After the detection of 10 batches of samples,the common mode of HPLC-ELSD fingerprint for total Steroid Saponins in herbs of D. zingiberensis was established for the first time,and 25 common peaks were determined. Among them, 10 peaks were identified by comparing with reference substances. The similarities of 10 batches of herbs were evaluated in the common mode. All of them were higher than 0. 80. This method is so accurate, reliable and highly repeatable that it can provide scientific basis for evaluating and controlling the quality of total Steroid Saponins in herbs of D. zingiberensis.