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Dalila Aldanaaranda - One of the best experts on this subject based on the ideXlab platform.
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epifluorescencia en la nutricion de larvas de Strombus gigas y Strombus pugilis mesogastropoda strombidae
Revista De Biologia Tropical, 2017Co-Authors: Dalila Aldanaaranda, Victoria PatinosuarezAbstract:In order to increase the scientific knowledge about the ingestion and digestion processes of the microalga Tetraselmis suecica by veliger larvae, we did a comparative analisis of feeding kinetics of two Caribbean conch larvae: Queen conch, Strombus gigas, and Pighting conch, Strombus pugilis. These kinetics have been determined in a direct way by means of epifluorescence microscopyc with this technique the potential nutritive value of different microalgae, can be detected in terms of their ingestion and digestion rates. Given the all to fluorescence of algal cells and transparency of both larvae tissues and shell, the algae within the stomach, their lisis and subsequent degradation are observed. The evolution of the ingestion and digestion processes can be follow in live larvae, where other events like the expulsion of intact algae cells initially ingested by the larvae can be observed. The nutritional effects of T. suecica on veliger larvae are explained infunction of a cromatic scale of four nutritional stages, and by means of temporal and absolute ingestion and digestion indices.
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overview of diets used in larviculture of three caribbean conchs queen conch Strombus gigas milk conch Strombus costatus and fighting conch Strombus pugilis
Aquaculture, 1998Co-Authors: Dalila Aldanaaranda, Victoria Patino SuarezAbstract:Abstract The genus Strombus is widely distributed in the Caribbean. Six species are of commercial importance: S. gigas, S. raninus, S. costatus, S. alatus, S. gallus and S. pugilis. Economically, the Queen conch, S. gigas is the most important and consequently the most widely studied. However, since 1970 a decline of S. gigas populations due to over-fishing has been observed. Many authors have studied S. gigas hatchery rearing techniques in order to address this problem; however, for these hatchery techniques to be successful, an adequate diet must be provided for the larvae. Some information of the nutritional requirements of S. gigas larvae have been reported since 1965, but a nutritionally complete diet is still not available. In this work we summarize the different diets that have been used for S. gigas, S. costatus and S. pugilis larvae rearing. Twenty one different algae species have been used: Amphidinium carteri, Chaetoceros gracilis, Dunaliella tertiolecta, Emillania huxleyi, Heterocapsa pygmacea, Isochrysis (Caicos), Isochrysis (Tahiti), Isochrysis sp., Monochrysis sp., Nannochloris, Nitzchia, Platymonas sp., P. tetraselmis, Prorocentrum minimun, Rhodomonas sp., Skeletonema costatus, Tetraselmis chuii, Tetraselmis sp., T. suecica, Thalassiosira fluviatilis and T. weissflogii. There are other diets that have seldom been studied with Strombus veliger larvae, that could be a potential food source for these gastropods. The type and concentration of algae, larval rearing conditions are summarized along with the results attained in larval growth, metamorphosis, survival, ingestion and digestion rates.
Olivier Gros - One of the best experts on this subject based on the ideXlab platform.
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Development of the planktotrophic veligers and plantigrades of Strombus pugilis (Gastropoda)
Journal of Molluscan Studies, 2015Co-Authors: M. R. Enriquez-diaz, Jean-marie Volland, J. F. Chavez-villegas, D. Aldana-aranda, Olivier GrosAbstract:The organogenesis, histogenesis and growth of larvae of the fighting conch Strombus pugilis (Linne, 1758) were studied over a period of 30 d after hatching in laboratory culture. Early development of S. pugilis was examined by light and scanning electron microscopy. Rearing was conducted at 27 +/- 1 degrees C. Veligers were reared at 200 larvae l(-1) in 4-l containers. Larvae were fed with the microalgae Isochrysis galbana and Nanochloropsis oculata at a concentration of 1,000 cell l(-1). The protoconch at hatching measured 212 +/- 12.14 mu m in length and the shell reached 1,100 +/- 29.11 mu m 29 d after hatching. Development characteristics are described from hatching to settlement. Newly hatched veligers possess two velar lobes, a larval shell consisting of 1.5 whorls, eyespots and a single right tentacle. Late veligers (5-d old larvae) have four velar lobes and two shell whorls and the left tentacle appears. Pediveligers show a functional adult heart at 11 d. Crawling behaviour and settlement were observed from 27 to 31 d. Plantigrades were observed after 29 d, when a functioning proboscis is observed and the velar lobes are lost. This study will facilitate the identification of gastropod larval shells in the plankton and of juveniles in the meiobenthos and will aid aquaculture of Strombus species.
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Development of the planktotrophic veligers and plantigrades of Strombus pugilis (Gastropoda)
Journal of Molluscan Studies, 2015Co-Authors: M. R. Enriquez-diaz, Jean-marie Volland, J. F. Chavez-villegas, D. Aldana-aranda, Olivier GrosAbstract:The organogenesis, histogenesis and growth of larvae of the fighting conch Strombus pugilis (Linne´ , 1758) were studied over a period of 30 d after hatching in laboratory culture. Early development of S. pugilis was examined by light and scanning electron microscopy. Rearing was conducted at 27+ 1 8C. Veligers were reared at 200 larvae l 21 in 4-l containers. Larvae were fed with the microalgae Isochrysis galbana and Nanochloropsis oculata at a concentration of 1,000 cell l 21 . The protoconch at hatching measured 212+ 12.14 mm in length and the shell reached 1,100+ 29.11 m m2 9 d after hatching. Development characteristics are described from hatching to settlement. Newly hatched veligers possess two velar lobes, a larval shell consisting of 1.5 whorls, eyespots and a single right tentacle. Late veligers (5-d old larvae) have four velar lobes and two shell whorls and the left tentacle appears. Pediveligers show a functional adult heart at 11 d. Crawling behaviour and settlement were observed from 27 to 31 d. Plantigrades were observed after 29 d, when a functioning proboscis is observed and the velar lobes are lost. This study will facilitate the identification of gastropod larval shells in the plankton and of juveniles in the meiobenthos and will aid aquaculture of Strombus species.
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Cytochemical Investigation of the Digestive Gland of Two Strombidae Species (Strombus gigas and Strombus pugilis) in Relation to the Nutrition
Microscopy Research and Technique, 2012Co-Authors: Jean-marie Volland, Olivier GrosAbstract:Strombus gigas and Strombus pugilis are threatened species and aquaculture represents a good alternative solution to the fishing. In this study, we highlighted the intracellular digestion process in the digestive gland of two Strombidae species, S. gigas and Strombus pugilis, by the cytochemical characterization of two lysosomal enzymes: acid phosphatase and arylsulfatase. In order to check the efficiency of artificial food digestion, we onducted the characterization on freshly collected, tarved and artificially fed individuals of S. pugilis. TEM observations of digestive gland sections from freshly collected individuals of both species revealed the resence of acid phosphatase and arylsulfatase activity mostly located in the apical third of digestive cells. Both enzymes were also detected in artificially fed individuals. In response to the starvation, acid phosphatase is not produced anymore by digestive cells, while arylsulfatase is still present. To our knowledge, this is the first cytochemical validation of intracellular digestion of artificial food in Strombidae. This study highlights the intracellular digestion of artificial food developed for Strombidae aquaculture. Moreover, we have shown that the lysosomal activity could be used as a feed index.
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Insight of EDX Analysis and EFTEM: Are Spherocrystals Located in Strombidae Digestive Gland Implied in Detoxification of Trace Metals ?
Microscopy Research and Technique, 2011Co-Authors: Jean-marie Volland, D. Aldana-aranda, Jean-pierre Lechaire, Ghislaine Frebourg, Gaëlle Ramdine, Olivier GrosAbstract:Digestive tubules of Strombidae are composed by three cell types: digestive cells, vacuolated cells, and crypt cells. The last one is characterized by the presence of intracellular granules identified as spherocrystals. Such structures are known to occur in basophilic cells of gastropod digestive gland, where they are supposed to be involved in the regulation of some minerals and in detoxification. In this study, energy-dispersive X-ray analysis (EDX) and energy filtered transmission electron microscopy (EFTEM) were used to determine the elemental content of spherocrystals in two Strombidae, Strombus gigas and Strombus pugilis. In freshly collected individuals of both species, the following elements were detected: Ca, Fe, Mg, P, and Zn. Aluminum and Mn were also detected in S. gigas. Their presence in spherocrystals indicates that, in Strombidae, spherocrystals are involved in the regulation of minerals and essential trace metals. In order to answer the question ''are spherocrystals involved in nonessential trace metals scavenging?,'' artificial cadmium and lead exposure by both waterborne and dietary pathways was applied to S. pugilis. No evidence of cadmium (Cd(NO3)2) or lead (Pb(NO3)2) provided by food was found in spherocrystals. Cadmium provided in water (Cd(NO3)2 and CdCl2) causes structural modifications of the digestive gland; however, this element was not trapped in spherocrystals. These results suggest that spherocrystals are not involved in detoxification of such nonessential trace metals.
Victoria Patino Suarez - One of the best experts on this subject based on the ideXlab platform.
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overview of diets used in larviculture of three caribbean conchs queen conch Strombus gigas milk conch Strombus costatus and fighting conch Strombus pugilis
Aquaculture, 1998Co-Authors: Dalila Aldanaaranda, Victoria Patino SuarezAbstract:Abstract The genus Strombus is widely distributed in the Caribbean. Six species are of commercial importance: S. gigas, S. raninus, S. costatus, S. alatus, S. gallus and S. pugilis. Economically, the Queen conch, S. gigas is the most important and consequently the most widely studied. However, since 1970 a decline of S. gigas populations due to over-fishing has been observed. Many authors have studied S. gigas hatchery rearing techniques in order to address this problem; however, for these hatchery techniques to be successful, an adequate diet must be provided for the larvae. Some information of the nutritional requirements of S. gigas larvae have been reported since 1965, but a nutritionally complete diet is still not available. In this work we summarize the different diets that have been used for S. gigas, S. costatus and S. pugilis larvae rearing. Twenty one different algae species have been used: Amphidinium carteri, Chaetoceros gracilis, Dunaliella tertiolecta, Emillania huxleyi, Heterocapsa pygmacea, Isochrysis (Caicos), Isochrysis (Tahiti), Isochrysis sp., Monochrysis sp., Nannochloris, Nitzchia, Platymonas sp., P. tetraselmis, Prorocentrum minimun, Rhodomonas sp., Skeletonema costatus, Tetraselmis chuii, Tetraselmis sp., T. suecica, Thalassiosira fluviatilis and T. weissflogii. There are other diets that have seldom been studied with Strombus veliger larvae, that could be a potential food source for these gastropods. The type and concentration of algae, larval rearing conditions are summarized along with the results attained in larval growth, metamorphosis, survival, ingestion and digestion rates.
A H Heuer - One of the best experts on this subject based on the ideXlab platform.
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tissue regeneration in the shell of the giant queen conch Strombus gigas
Chemistry of Materials, 2004Co-Authors: Dong Mei Zhang, A H HeuerAbstract:The crossed-lamellar microarchitecture (microstructure) of the shell of Strombus gigas, the giant Queen conch native to Caribbean habitats, is the most common of the several shell microarchitecture...
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Structural basis for the fracture toughness of the shell of the conch Strombus gigas
Nature, 2000Co-Authors: S. Kamat, Roberto Ballarini, A H HeuerAbstract:Natural composite materials are renowned for their mechanical strength and toughness: despite being highly mineralized, with the organic component constituting not more than a few per cent of the composite material, the fracture toughness exceeds that of single crystals of the pure mineral by two to three orders of magnitude. The judicious placement of the organic matrix, relative to the mineral phase, and the hierarchical structural architecture extending over several distinct length scales both play crucial roles in the mechanical response of natural composites to external loads. Here we use transmission electron microscopy studies and beam bending experiments to show that the resistance of the shell of the conch Strombus gigas to catastrophic fracture can be understood quantitatively by invoking two energy-dissipating mechanisms: multiple microcracking in the outer layers at low mechanical loads, and crack bridging in the shell's tougher middle layers at higher loads. Both mechanisms are intimately associated with the so-called crossed lamellar microarchitecture of the shell, which provides for 'channel' cracking in the outer layers and uncracked structural features that bridge crack surfaces, thereby significantly increasing the work of fracture, and hence the toughness, of the material. Despite a high mineral content of about 99% (by volume) of aragonite, the shell of Strombus gigas can thus be considered a 'ceramic plywood' and can guide the biomimetic design of tough, lightweight structures.
Zaidi Che Cob - One of the best experts on this subject based on the ideXlab platform.
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Description and evaluation of imposex in Strombus canarium Linnaeus, 1758 (Gastropoda, Strombidae): a potential bio-indicator of tributyltin pollution.
Environmental monitoring and assessment, 2010Co-Authors: Zaidi Che Cob, Aziz Arshad, Japar Sidik Bujang, Mazlan Abd GhaffarAbstract:Strombus canarium Linnaeus, 1758 is an important gastropod species within the study area and was traditionally collected for food by the locals. The objective of the present study is to assess the incidence of imposex and its severity in this species. Adult conchs were sampled during their main reproductive period, from October 2005 to January 2006, at Sungai Pulai estuary, Johor Straits, Malaysia. A total of 32.81% of adult females showed imposex characteristics, with varying degrees of severity though. The relative penis size (RPS) index ranged from 1.74 to 33.29 (mean = 13.40 ± 2.27, n = 21), while the relative penis length (RPL) index ranged from 6.28 to 55.19 (mean = 25.83 ± 3.33, n = 21). The use of vas deferens sequence (VDS) index was however cannot be applied as the presence of egg groove obscured any vas deferens development in affected females. Sequence of imposex (male penis) development in female conch, from merely a small stump to an advance male penis homologous was therefore carefully analyzed and described, and an alternative imposex classification scheme was proposed. S. canarium can be a good indicator for monitoring of organotin pollution within the study area. However, more studies are needed in order to further develop and test its validity and application, such as its correlation with levels of pollutant within the tissues and the environment, as well as its application on other Strombus species.
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metamorphosis induction of the dog conch Strombus canarium gastropoda strombidae using cues associated with conch nursery habitat
Journal of Applied Sciences, 2010Co-Authors: Zaidi Che Cob, Aziz Arshad, Japar Sidik Bujang, Wan Mohd. Lotfi Wan Muda, Mazlan Abd GhaffarAbstract:Strombus canarium is a commercially important gastropod that has great potential for advancement into aquaculture. In this study, the metamorphosis response of Strombus canarium larvae to various metamorphosis cues associated with conch nursery habitat and to KCl and GABA, were tested. Bioassays were run as static, no choice experiment and adopting a continuous exposure approach. Strombus canarium larvae showed strong metamorphosis responses when sediment (i.e., conch nursery habitat sediment/SD-NU) and detrital substrata (i.e., Thalassia detritus leachate/T-LC) from their nursery habitat were used (p<0.05). There was no metamorphosis in treatments using sterilized conch nursery habitat sediment (SD-ST) and sediment taken from outside conch nursery habitat (SD-OT). Experiments using fresh macrophyte blades of Enhalus acoroides (EA), Thalassia hemprichii (TH), Halophila ovalis (HA) and Ulva (UL) and adult conditioned seawater (SD-SW) also showed negative respond. Conch larvae demonstrate active habitat selection during metamorphosis and no spontaneous metamorphosis was observed. Settlement in S. canarium is associative in nature where epibionts associated with conch nursery habitat could be the cue for the metamorphosis. However, the specific epibionts/inducers and mechanisms underlining the process were not studied and therefore are subjected to more detailed investigation. The use of KCl was comparable with treatments using natural inducers (SD-NU and T-LC), thus was suggested for application in hatchery spat production of the species.
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Metamorphosis induction of the dog conch Strombus canarium (Gastropoda: Strombidae) using cues associated with conch nursery habitat.
Journal of Applied Sciences, 2010Co-Authors: Zaidi Che Cob, Aziz Arshad, Japar Sidik Bujang, Wan Mohd. Lotfi Wan Muda, Mazlan Abd GhaffarAbstract:Strombus canarium is a commercially important gastropod that has great potential for advancement into aquaculture. In this study, the metamorphosis response of Strombus canarium larvae to various metamorphosis cues associated with conch nursery habitat and to KCl and GABA, were tested. Bioassays were run as static, no choice experiment and adopting a continuous exposure approach. Strombus canarium larvae showed strong metamorphosis responses when sediment (i.e., conch nursery habitat sediment/SD-NU) and detrital substrata (i.e., Thalassia detritus leachate/T-LC) from their nursery habitat were used (p
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species description and distribution of Strombus mollusca strombidae in johor straits and its surrounding areas
Sains Malaysiana, 2009Co-Authors: Zaidi Che Cob, Aziz Arshad, Japar Sidik Bujang, Mazlan Abd GhaffarAbstract:A total of 230 individuals of Strombus were sampled at various locations along the Johor Straits, Malaysia. There were four species of Strombus present in the study areas i.e. Strombus canarium Linnaeus, 1758; Strombus urceus Linnaeus, 1758; Strombus marginatus subspecies succinctus Linnaeus, 1767; Strombus marginatus subspecies robustus Sowerby, 1874; and Strombus vittatus subspecies vittatus Linnaeus, 1758. Strombus canarium was the most common, widely distributed and most abundant, followed by S. urceus, while the others were only rarely found. Among the species Strombus marginatus and Strombus vittatus were two new distribution records for the Johor Straits. Since all Strombus were traditionally harvested and consumed by the locals since long ago, further studies are needed particularly regarding the population dynamics and fishery of the harvested species.
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Development and growth of larvae of the dog conch, Strombus canarium (Mollusca: Gastropoda), in the laboratory
Zoological Studies, 2009Co-Authors: Zaidi Che Cob, Aziz Arshad, Japar Sidik Bujang, Mazlan Abd Ghaffar, Wan Mohd. Lotfi Wan MudaAbstract:Development and growth of larvae of the dog conch, Strombus canarium (Mollusca: Gastropoda), in the laboratory. Zoological Studies 48(1): 1-11. Strombus canarium egg masses used in this study were collected while still underneath spawning females, and embryonic and post-hatching larval development was observed in the laboratory. Strombus canarium larvae were reared at 200 larvae/L in 2-L containers with 0.22 μm filtered seawater medium at a salinity of 30 ± 1 PSU, and fed a single algal species, lsochrysis galbana, at 1000 cells/ml. Fecundity was estimated at 48,745 + 877 to 93,643 + 1685 (n = 10) eggs/egg mass. The incubation time was between 110.4 and 134.4 h (mean 122.43 + 3.07 h, n = 7) at 29 ± 1°C, with a high hatching rate (mean 93.34% ± 1.68% hatched, n = 3). The larvae have 2 velar lobes and 1.5 shell whorls at the time of hatching, with an average shell length of 216.77 ± 5.72 μm (n = 10). Based on prominent larval characters and visible morphological features, S. canarium larvae can be assigned to 4 different development stages, i.e. stages I, II, III, and IV, which are described in this paper. The larvae reached metamorphic competence at 17-23 d post-hatching, and only metamorphosed when settlement cues (sediments from its natural habitat and 15 mM KCI) were introduced. They showed a short period of metamorphic competence, and no spontaneous metamorphosis was observed. Morphological changes and larval behaviors at the onset and during metamorphosis are also described. http://zoolstud.sinica.edu.tw/Journals/48.1/1.pdf
