The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Lazar Mathew - One of the best experts on this subject based on the ideXlab platform.
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aqueous extract of phyllanthus amarus inhibits chromium vi induced toxicity in mda mb 435s cells
Food and Chemical Toxicology, 2010Co-Authors: Gunjan Guha, V Rajkumar, Ashok R Kumar, Lazar MathewAbstract:Abstract Cr(VI) (hexavalent) is a very Strong Oxidant which causes high cytotoxicity through oxidative stress in tissue systems. Its abundance in groundwater and drinking water in several parts of the world has been noted to cause severe toxicity to both flora and fauna. This study evaluated the effects of aqueous extract of Phyllanthus amarus Schum. & Thon. against Cr(VI)-induced oxidative toxicity in vitro in MDA-MB-435S human breast carcinoma cells, along with an estimation of its antiOxidant potential, inhibition of lipid peroxidation and determination of its polyphenolic composition. The extract showed significant ( P and ABTS + ) and Fe +3 , and in inhibiting lipid peroxidation. A distinct decline in Cr(VI)-induced cytotoxicity was noticed in MDA-MB-435S cells with an increase in extract dosage. Furthermore, the extract proved to contain a high content of phenolic compounds which were found to have Strong and significant ( P P. amarus aqueous extract has high antiOxidant potential (by virtue of its phenolic constituents) which simultaneously inhibits Cr(VI)-induced oxidative toxicity to MDA-MB-435S cells.
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aqueous extract of phyllanthus amarus inhibits chromium vi induced toxicity in mda mb 435s cells
Food and Chemical Toxicology, 2010Co-Authors: Gunjan Guha, V Rajkumar, Ashok R Kumar, Lazar MathewAbstract:Cr(VI) (hexavalent) is a very Strong Oxidant which causes high cytotoxicity through oxidative stress in tissue systems. Its abundance in groundwater and drinking water in several parts of the world has been noted to cause severe toxicity to both flora and fauna. This study evaluated the effects of aqueous extract of Phyllanthus amarus Schum. & Thon. against Cr(VI)-induced oxidative toxicity in vitro in MDA-MB-435S human breast carcinoma cells, along with an estimation of its antiOxidant potential, inhibition of lipid peroxidation and determination of its polyphenolic composition. The extract showed significant (P<0.05) potential in scavenging free radicals (DPPH() and ABTS()(+)) and Fe(+3), and in inhibiting lipid peroxidation. A distinct decline in Cr(VI)-induced cytotoxicity was noticed in MDA-MB-435S cells with an increase in extract dosage. Furthermore, the extract proved to contain a high content of phenolic compounds which were found to have Strong and significant (P<0.05) positive correlations to free-radical scavenging potential, lipid peroxidation inhibition capacity and cyto-protective efficiency against Cr(VI)-induced oxidative cellular damage. HPLC analysis identified some of the major phenolic compounds in it. It was concluded that P. amarus aqueous extract has high antiOxidant potential (by virtue of its phenolic constituents) which simultaneously inhibits Cr(VI)-induced oxidative toxicity to MDA-MB-435S cells.
Gunjan Guha - One of the best experts on this subject based on the ideXlab platform.
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aqueous extract of phyllanthus amarus inhibits chromium vi induced toxicity in mda mb 435s cells
Food and Chemical Toxicology, 2010Co-Authors: Gunjan Guha, V Rajkumar, Ashok R Kumar, Lazar MathewAbstract:Abstract Cr(VI) (hexavalent) is a very Strong Oxidant which causes high cytotoxicity through oxidative stress in tissue systems. Its abundance in groundwater and drinking water in several parts of the world has been noted to cause severe toxicity to both flora and fauna. This study evaluated the effects of aqueous extract of Phyllanthus amarus Schum. & Thon. against Cr(VI)-induced oxidative toxicity in vitro in MDA-MB-435S human breast carcinoma cells, along with an estimation of its antiOxidant potential, inhibition of lipid peroxidation and determination of its polyphenolic composition. The extract showed significant ( P and ABTS + ) and Fe +3 , and in inhibiting lipid peroxidation. A distinct decline in Cr(VI)-induced cytotoxicity was noticed in MDA-MB-435S cells with an increase in extract dosage. Furthermore, the extract proved to contain a high content of phenolic compounds which were found to have Strong and significant ( P P. amarus aqueous extract has high antiOxidant potential (by virtue of its phenolic constituents) which simultaneously inhibits Cr(VI)-induced oxidative toxicity to MDA-MB-435S cells.
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aqueous extract of phyllanthus amarus inhibits chromium vi induced toxicity in mda mb 435s cells
Food and Chemical Toxicology, 2010Co-Authors: Gunjan Guha, V Rajkumar, Ashok R Kumar, Lazar MathewAbstract:Cr(VI) (hexavalent) is a very Strong Oxidant which causes high cytotoxicity through oxidative stress in tissue systems. Its abundance in groundwater and drinking water in several parts of the world has been noted to cause severe toxicity to both flora and fauna. This study evaluated the effects of aqueous extract of Phyllanthus amarus Schum. & Thon. against Cr(VI)-induced oxidative toxicity in vitro in MDA-MB-435S human breast carcinoma cells, along with an estimation of its antiOxidant potential, inhibition of lipid peroxidation and determination of its polyphenolic composition. The extract showed significant (P<0.05) potential in scavenging free radicals (DPPH() and ABTS()(+)) and Fe(+3), and in inhibiting lipid peroxidation. A distinct decline in Cr(VI)-induced cytotoxicity was noticed in MDA-MB-435S cells with an increase in extract dosage. Furthermore, the extract proved to contain a high content of phenolic compounds which were found to have Strong and significant (P<0.05) positive correlations to free-radical scavenging potential, lipid peroxidation inhibition capacity and cyto-protective efficiency against Cr(VI)-induced oxidative cellular damage. HPLC analysis identified some of the major phenolic compounds in it. It was concluded that P. amarus aqueous extract has high antiOxidant potential (by virtue of its phenolic constituents) which simultaneously inhibits Cr(VI)-induced oxidative toxicity to MDA-MB-435S cells.
V Rajkumar - One of the best experts on this subject based on the ideXlab platform.
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aqueous extract of phyllanthus amarus inhibits chromium vi induced toxicity in mda mb 435s cells
Food and Chemical Toxicology, 2010Co-Authors: Gunjan Guha, V Rajkumar, Ashok R Kumar, Lazar MathewAbstract:Abstract Cr(VI) (hexavalent) is a very Strong Oxidant which causes high cytotoxicity through oxidative stress in tissue systems. Its abundance in groundwater and drinking water in several parts of the world has been noted to cause severe toxicity to both flora and fauna. This study evaluated the effects of aqueous extract of Phyllanthus amarus Schum. & Thon. against Cr(VI)-induced oxidative toxicity in vitro in MDA-MB-435S human breast carcinoma cells, along with an estimation of its antiOxidant potential, inhibition of lipid peroxidation and determination of its polyphenolic composition. The extract showed significant ( P and ABTS + ) and Fe +3 , and in inhibiting lipid peroxidation. A distinct decline in Cr(VI)-induced cytotoxicity was noticed in MDA-MB-435S cells with an increase in extract dosage. Furthermore, the extract proved to contain a high content of phenolic compounds which were found to have Strong and significant ( P P. amarus aqueous extract has high antiOxidant potential (by virtue of its phenolic constituents) which simultaneously inhibits Cr(VI)-induced oxidative toxicity to MDA-MB-435S cells.
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aqueous extract of phyllanthus amarus inhibits chromium vi induced toxicity in mda mb 435s cells
Food and Chemical Toxicology, 2010Co-Authors: Gunjan Guha, V Rajkumar, Ashok R Kumar, Lazar MathewAbstract:Cr(VI) (hexavalent) is a very Strong Oxidant which causes high cytotoxicity through oxidative stress in tissue systems. Its abundance in groundwater and drinking water in several parts of the world has been noted to cause severe toxicity to both flora and fauna. This study evaluated the effects of aqueous extract of Phyllanthus amarus Schum. & Thon. against Cr(VI)-induced oxidative toxicity in vitro in MDA-MB-435S human breast carcinoma cells, along with an estimation of its antiOxidant potential, inhibition of lipid peroxidation and determination of its polyphenolic composition. The extract showed significant (P<0.05) potential in scavenging free radicals (DPPH() and ABTS()(+)) and Fe(+3), and in inhibiting lipid peroxidation. A distinct decline in Cr(VI)-induced cytotoxicity was noticed in MDA-MB-435S cells with an increase in extract dosage. Furthermore, the extract proved to contain a high content of phenolic compounds which were found to have Strong and significant (P<0.05) positive correlations to free-radical scavenging potential, lipid peroxidation inhibition capacity and cyto-protective efficiency against Cr(VI)-induced oxidative cellular damage. HPLC analysis identified some of the major phenolic compounds in it. It was concluded that P. amarus aqueous extract has high antiOxidant potential (by virtue of its phenolic constituents) which simultaneously inhibits Cr(VI)-induced oxidative toxicity to MDA-MB-435S cells.
Ashok R Kumar - One of the best experts on this subject based on the ideXlab platform.
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aqueous extract of phyllanthus amarus inhibits chromium vi induced toxicity in mda mb 435s cells
Food and Chemical Toxicology, 2010Co-Authors: Gunjan Guha, V Rajkumar, Ashok R Kumar, Lazar MathewAbstract:Abstract Cr(VI) (hexavalent) is a very Strong Oxidant which causes high cytotoxicity through oxidative stress in tissue systems. Its abundance in groundwater and drinking water in several parts of the world has been noted to cause severe toxicity to both flora and fauna. This study evaluated the effects of aqueous extract of Phyllanthus amarus Schum. & Thon. against Cr(VI)-induced oxidative toxicity in vitro in MDA-MB-435S human breast carcinoma cells, along with an estimation of its antiOxidant potential, inhibition of lipid peroxidation and determination of its polyphenolic composition. The extract showed significant ( P and ABTS + ) and Fe +3 , and in inhibiting lipid peroxidation. A distinct decline in Cr(VI)-induced cytotoxicity was noticed in MDA-MB-435S cells with an increase in extract dosage. Furthermore, the extract proved to contain a high content of phenolic compounds which were found to have Strong and significant ( P P. amarus aqueous extract has high antiOxidant potential (by virtue of its phenolic constituents) which simultaneously inhibits Cr(VI)-induced oxidative toxicity to MDA-MB-435S cells.
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aqueous extract of phyllanthus amarus inhibits chromium vi induced toxicity in mda mb 435s cells
Food and Chemical Toxicology, 2010Co-Authors: Gunjan Guha, V Rajkumar, Ashok R Kumar, Lazar MathewAbstract:Cr(VI) (hexavalent) is a very Strong Oxidant which causes high cytotoxicity through oxidative stress in tissue systems. Its abundance in groundwater and drinking water in several parts of the world has been noted to cause severe toxicity to both flora and fauna. This study evaluated the effects of aqueous extract of Phyllanthus amarus Schum. & Thon. against Cr(VI)-induced oxidative toxicity in vitro in MDA-MB-435S human breast carcinoma cells, along with an estimation of its antiOxidant potential, inhibition of lipid peroxidation and determination of its polyphenolic composition. The extract showed significant (P<0.05) potential in scavenging free radicals (DPPH() and ABTS()(+)) and Fe(+3), and in inhibiting lipid peroxidation. A distinct decline in Cr(VI)-induced cytotoxicity was noticed in MDA-MB-435S cells with an increase in extract dosage. Furthermore, the extract proved to contain a high content of phenolic compounds which were found to have Strong and significant (P<0.05) positive correlations to free-radical scavenging potential, lipid peroxidation inhibition capacity and cyto-protective efficiency against Cr(VI)-induced oxidative cellular damage. HPLC analysis identified some of the major phenolic compounds in it. It was concluded that P. amarus aqueous extract has high antiOxidant potential (by virtue of its phenolic constituents) which simultaneously inhibits Cr(VI)-induced oxidative toxicity to MDA-MB-435S cells.
Yasukazu Ejima - One of the best experts on this subject based on the ideXlab platform.
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novel absorbance peak of gentisic acid following the oxidation reaction
PLOS ONE, 2020Co-Authors: Sho Hosokawa, Kenichi Shukuya, Keisuke Sogabe, Yasukazu Ejima, Tatsuya Morinishi, Eiichiro Hirakawa, Tatsuo Shimosawa, Hiroyuki Ohsaki, Yasunori TokuharaAbstract:: Gentisic acid (GA), a metabolite of acetylsalicylic acid (ASA), and homogentisic acid (HGA), which is excreted at high levels in alkaptonuria, are divalent phenolic acids with very similar structures. Urine containing HGA is dark brown in color due to its oxidation. We recently reported a new oxidation method of HGA involving the addition of sodium hydroxide (NaOH) with sodium hypochlorite pentahydrate (NaOCl·5H2O), which is a Strong Oxidant. In the present study, we attempted to oxidize GA, which has a similar structure to HGA, using our method. We herein observed color changes in GA solution and analyzed the absorption spectra of GA after the addition of NaOH with NaOCl·5H2O. We also examined the oxidation reaction of GA using a liquid chromatography time-of-flight mass spectrometer (LC/TOF-MS). The results obtained indicated that GA solution had a unique absorption spectrum with a peak at approximately 500 nm through an oxidation reaction following the addition of NaOH with NaOCl·5H2O. This spectrophotometric method enables GA to be detected in sample solutions without expensive analytical instruments or a complex method.
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absorbance measurements of oxidation of homogentisic acid accelerated by the addition of alkaline solution with sodium hypochlorite pentahydrate
Scientific Reports, 2018Co-Authors: Yasunori Tokuhara, Sho Hosokawa, Kenichi Shukuya, Keisuke Sogabe, Yasukazu Ejima, Tatsuya Morinishi, Eiichiro Hirakawa, Hiroyuki Ohsaki, Masami Tanaka, Yutaka YatomiAbstract:The urine of patients with alkaptonuria turns dark brown due to the oxidation of homogentisic acid (HGA) to benzoquinone acetic acid (BQA), and this is accelerated by the addition of alkali. We recently reported that alkaptonuric urine and HGA after the addition of alkali showed characteristic peaks at 406 and 430 nm. In order to improve the sensitivity of our spectrometric method for the detection of HGA, we accelerated the oxidation of HGA to BQA using sodium hypochlorite pentahydrate (NaOCl·5H2O), which is a Strong Oxidant. In the present study, we measured the absorption spectra of alkaptonuric urine and HGA solution after the addition of sodium hydroxide (NaOH) or NaOH with NaOCl·5H2O and analyzed the oxidation reaction of HGA after alkalization using a liquid chromatography time-of-flight mass spectrometer (LC/TOF-MS) and nuclear magnetic resonance (NMR) spectrometry. We accelerated the oxidation of HGA to BQA by adding NaOH with NaOCl·5H2O, and this absorbance measurement was useful for more sensitively observing the oxidation of HGA than LC/TOF-MS and NMR spectroscopy. This quick and easy screening method may be suitable for the diagnosis of alkaptonuria.
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practical oxidative dearomatization of phenols with sodium hypochlorite pentahydrate
ChemInform, 2015Co-Authors: Muhammet Uyanik, Yasukazu Ejima, Niiha Sasakura, Mitsuyoshi Kuwahata, Kazuaki IshiharaAbstract:First time, the title reaction proceeds in the presence of sodium hypochlorite pentahydrate as an inexpensive, Strong Oxidant.
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practical oxidative dearomatization of phenols with sodium hypochlorite pentahydrate
Chemistry Letters, 2015Co-Authors: Muhammet Uyanik, Yasukazu Ejima, Niiha Sasakura, Mitsuyoshi Kuwahata, Kazuaki IshiharaAbstract:A highly efficient and practical oxidative dearomatization of phenols using sodium hypochlorite pentahydrate as an inexpensive, Strong Oxidant is reported for the first time. The oxidation reaction...
