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Majeti Narsimha Vara Prasad - One of the best experts on this subject based on the ideXlab platform.
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Strychnos Nux-Vomica Root Extract Induces Apoptosis in the Human Multiple Myeloma Cell Line—U266B1
Cell Biochemistry and Biophysics, 2013Co-Authors: Majeti Narsimha Vara PrasadAbstract:Multiple myeloma (MM) is an incurable B cell neoplasm causing significant morbidity and mortality. Despite recent advances in the MM-treatment, the disease still remains incurable; necessitating a search for new therapeutic agents. Therefore, Strychnos Nux - Vomica L. root extract (SN) was screened using the human MM-cell line, U266B1. SN-extract demonstrated anti-proliferative effect in a time- and dose-dependent manner. Morphological studies, cell cycle analysis by FACS indicate apoptosis. Furthermore, assessment of signaling molecules like IL-6 (interleukin-6) and CD-138 ( Sydencan-1) confirmed apoptosis. The anti-proliferative activity of SN-extract is associated with apoptosis, which is likely due to the presence of alkaloids, strychnine and brucine, which have been identified by MALDI-TOF-MS and RP-HPLC analysis
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Strychnos Nux Vomica root extract induces apoptosis in the human multiple myeloma cell line u266b1
Cell Biochemistry and Biophysics, 2013Co-Authors: Majeti Narsimha Vara PrasadAbstract:Multiple myeloma (MM) is an incurable B cell neoplasm causing significant morbidity and mortality. Despite recent advances in the MM-treatment, the disease still remains incurable; necessitating a search for new therapeutic agents. Therefore, Strychnos Nux-Vomica L. root extract (SN) was screened using the human MM-cell line, U266B1. SN-extract demonstrated anti-proliferative effect in a time- and dose-dependent manner. Morphological studies, cell cycle analysis by FACS indicate apoptosis. Furthermore, assessment of signaling molecules like IL-6 (interleukin-6) and CD-138 (Sydencan-1) confirmed apoptosis. The anti-proliferative activity of SN-extract is associated with apoptosis, which is likely due to the presence of alkaloids, strychnine and brucine, which have been identified by MALDI-TOF-MS and RP-HPLC analysis
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anti proliferative and cytotoxic effects of Strychnos Nux Vomica root extract on human multiple myeloma cell line rpmi 8226
Food and Chemical Toxicology, 2009Co-Authors: Madduri Ramanadham, Majeti Narsimha Vara PrasadAbstract:Multiple myeloma (MM) is an incurable hematological malignancy with high incidence in the elderly. The currently used chemotherapeutic drugs show severe side effects, dose-limiting toxicity and development of resistance. In search of novel plant derived anti-cancer agents, Strychnos Nux-Vomica L. (SN) root extract was screened using the human MM-cell line, RPMI 8226. SN-extract exhibited anti-proliferative activity in a dose and time dependent manner. The morphological assessment of SN-extract treated cells showed significant features associated with apoptosis. Cell cycle analysis using flow cytometry of cells stained with propidium iodide revealed accumulation of cells at sub-G0/G1 phase. In addition, disruption of mitochondrial membrane potential and subsequent leakage of mitochondrial cytochrome c was observed in SN-extract treated myeloma cells. The anti-proliferative and cytotoxic activity could be due to the alkaloids strychnine and brucine, which have been identified by LC–mass spectral analysis of the SN-extract in comparison to the reference standards analyzed under identical conditions.
Zhongzhen Zhao - One of the best experts on this subject based on the ideXlab platform.
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alkaloid profiling in crude and processed Strychnos Nux Vomica seeds by matrix assisted laser desorption ionization time of flight mass spectrometry
Journal of Pharmaceutical and Biomedical Analysis, 2007Co-Authors: Wei Wu, Chunfeng Qiao, Zhitao Liang, Hongxi Xu, Zhongzhen ZhaoAbstract:Abstract Direct analysis of alkaloids in the tissues of crude and processed Strychnos Nux-Vomica seeds by MALDI-TOFMS was described. The alkaloid profiles of the herb drugs were obtained without the need of complicated sample preparation to avoid potential damage or change of the active components. Seed tissues that were optimally sliced to a thickness of 10–20 μm from the crude and processed Strychnos Nux-Vomica seeds as well as various parts of tissue such as endosperm and epidermis were analyzed on MALDI target plate after the matrix was directly applied onto the tissue surface. The obtained alkaloid profiles provided valuable information for the differentiation of crude and processed Strychnos Nux-Vomica seeds and for the explanation of the significantly different toxicity. Experimental results indicated that the direct MALDI-TOFMS analysis allowed rapid screening of the alkaloid components in Strychnos Nux-Vomica seeds.
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Alkaloid profiling in crude and processed Strychnos Nux-Vomica seeds by matrix-assisted laser desorption/ionization-time of flight mass spectrometry
Journal of Pharmaceutical and Biomedical Analysis, 2007Co-Authors: Wei Wu, Chunfeng Qiao, Zhitao Liang, Hongxi Xu, Zhongzhen ZhaoAbstract:Abstract Direct analysis of alkaloids in the tissues of crude and processed Strychnos Nux-Vomica seeds by MALDI-TOFMS was described. The alkaloid profiles of the herb drugs were obtained without the need of complicated sample preparation to avoid potential damage or change of the active components. Seed tissues that were optimally sliced to a thickness of 10–20 μm from the crude and processed Strychnos Nux-Vomica seeds as well as various parts of tissue such as endosperm and epidermis were analyzed on MALDI target plate after the matrix was directly applied onto the tissue surface. The obtained alkaloid profiles provided valuable information for the differentiation of crude and processed Strychnos Nux-Vomica seeds and for the explanation of the significantly different toxicity. Experimental results indicated that the direct MALDI-TOFMS analysis allowed rapid screening of the alkaloid components in Strychnos Nux-Vomica seeds.
S Chaurasia - One of the best experts on this subject based on the ideXlab platform.
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interaction of Strychnos Nux Vomica products and iron with reference to lipid peroxidation
Phytomedicine, 2000Co-Authors: Yamini B Tripathi, S ChaurasiaAbstract:Summary In this paper, it has been investigated that strychnine, the major active principle in the alcoholic extract of the seeds of Strychnos Nux-Vomica, is responsible for its antilipid peroxidative property. The mechanism of action of this drug is through the chelation of the free iron in the system. It has also been observed that strychnine does not have any pro-oxidant-property, because it does not convert Fe3+ to Fe2+ and vice versa in the reaction system, as has been observed with several other antioxidants.
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studies on the inhibitory effect of Strychnos Nux Vomica alcohol extract on iron induced lipid peroxidation
Phytomedicine, 1996Co-Authors: Yamini B Tripathi, S ChaurasiaAbstract:Summary This report investigates the antioxidant property of Strychnos Nux Vomica Linn, alcohol extract on FeS04-induced lipid peroxidation in vitro. The results have been compared to those of vitamin E, parabenzoquinone, Tris, mannitol and EDTA. Tris and mannitol, the standard hydroxyl trappers failed to block this process. Interestingly EDTA, a strong metal chelator, significantly blocks the process of lipid peroxidation. S. Nux Vomica inhibits the lipid peroxidation in the dose dependent manner. The inhibition of lipid peroxidation was reversed by adding high concentration of Fe2+. This suggests the mechanism of action of S. Nux Vomica through the chelation of Fe++/Fe+++ ion in the system and not by trapping the hydroxyl radicals. This extract significantly maintains the hepatic glutathione content also in the time and dose dependent manner even in the presence of FeSO4, which is not the case with EDTA. It also inhibits the process of lipid peroxidation once induced.
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effect of Strychnos Nux Vomica alcohol extract on lipid peroxidation in rat liver
International Journal of Pharmacognosy, 1996Co-Authors: Yamini B Tripathi, S ChaurasiaAbstract:This report investigates the antioxidant properties of the purified Strychnos Nux Vomica L. seed extract for protection against non-enzymatic formation of lipid peroxides and on reduced glutathione (GSH) levels in rat liver homogenate. Results were compared with the natural antioxidant vitamin E. For the first time it could be reported that this drug protects against cumene hydroperoxide (CHP) induced lipid peroxidation in a dose dependent manner. It also inhibits the process of the lipid peroxidation, once induced. The drug significantly maintains the hepatic content of glutathione in a dose and time dependent manner, even in the presence of the above toxin (CHP). Thus it appears that S. Nux Vomica is a potent antioxidant and the mechanism of action could be through the scavenging of free radicals.
Michel Frederich - One of the best experts on this subject based on the ideXlab platform.
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dimeric bisindole alkaloids from the stem bark of Strychnos Nux Vomica l
Phytochemistry, 2013Co-Authors: Mariecaroline Jonville, Monique Tits, Luc Angenot, Georges Dive, Joanne Bero, Evelyne Ollivier, Michel FrederichAbstract:Strychnos Nux-Vomica L. (Loganiaceae) is famous for its monomeric alkaloid content, such as strychnine, a convulsant poison. The stem bark of the tree is traditionally used to treat intermittent fever in South East Asia. In various studies, it appeared that dimeric indolo-monoterpenic alkaloids possess a promising activity on Plasmodium falciparum. Three bisindolomonoterpenic alkaloids together with strychnochrysine, previously identified in the root bark of S. Nux-Vomica, were isolated from the stem bark. The structures of these compounds were established using NMR spectroscopy and mass spectrometry. Stereochemistry of the compounds was confirmed by molecular modelling. This then allowed the structural determination of strychnoflavine, a coloured bisindole alkaloid previously isolated from the root bark of the tree. Moreover, the conformational inversion in alkaloids possessing an ether bond in the strychnane moiety could be easily predicted by specific δ 13 C NMR values. These longicaudatine-type alkaloids were found to display in vitro antiplasmodial activity against a chloroquine resistant strain and a chloroquine sensitive strain. The most interesting was strychnochrysine showing an IC 50 value at around 10 μM. © 2012 Elsevier Ltd. All rights reserved.
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metabolomic analysis of Strychnos Nux Vomica Strychnos icaja and Strychnos ignatii extracts by 1h nuclear magnetic resonance spectrometry and multivariate analysis techniques
Phytochemistry, 2004Co-Authors: Michel Frederich, Luc Angenot, Young Hae Choi, Goetz Harnischfeger, Alfons W M Lefeber, Robert VerpoorteAbstract:Abstract 1 H Nuclear magnetic resonance spectrometry and multivariate analysis techniques were applied for the metabolic profiling of three Strychnos species: Strychnos Nux-Vomica (seeds, stem bark, root bark), Strychnos ignatii (seeds), and Strychnos icaja (leaves, stem bark, root bark, collar bark). The principal component analysis (PCA) of the 1 H NMR spectra showed a clear discrimination between all samples, using the three first components. The key compounds responsible for the discrimination were brucine, loganin, fatty acids, and Strychnos icaja alkaloids such as icajine and sungucine. The method was then applied to the classification of several “false angostura” samples. These samples were, as expected, identified as S. Nux-Vomica by PCA, but could not be clearly discriminated as root bark or stem bark samples after further statistical analysis.
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quantitative analysis of strychnine and brucine in Strychnos Nux Vomica using 1h nmr
Planta Medica, 2003Co-Authors: Michel Frederich, Young Hae Choi, Robert VerpoorteAbstract:A quantitative analysis using 1 H-NMR (Q-NMR) has been developed for the determination of strychnine and brucine in Strychnos Nux-Vomica seeds and stems. The advantages of the method are that no reference alkaloids are needed for calibration curves, the quantification could be directly realized on a crude extract, strychnine and brucine could easily be distinguished, an overall profile of the preparation (including non alkaloid compounds) could be directly obtained, and a very significant time-gain could be achieved, in comparison to conventional HPLC methods, for instance.
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strychnochrysine a new bisindole alkaloid from the roots of Strychnos Nux Vomica
Journal of Natural Products, 1998Co-Authors: Rose Gadi Biala, Monique Tits, Jacques Penelle, Michel Frederich, Viviane Brandt, Christelle Prosperi, Gabriel Llabres, Luc AngenotAbstract:The reinvestigation of Strychnos Nux-Vomica resulted in the isolation of a colored monoquaternary bisindole alkaloid from the roots. The structure of this new orange substance, strychnochrysine (1), was defined by detailed spectroscopic methods.
Wei Wu - One of the best experts on this subject based on the ideXlab platform.
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alkaloid profiling in crude and processed Strychnos Nux Vomica seeds by matrix assisted laser desorption ionization time of flight mass spectrometry
Journal of Pharmaceutical and Biomedical Analysis, 2007Co-Authors: Wei Wu, Chunfeng Qiao, Zhitao Liang, Hongxi Xu, Zhongzhen ZhaoAbstract:Abstract Direct analysis of alkaloids in the tissues of crude and processed Strychnos Nux-Vomica seeds by MALDI-TOFMS was described. The alkaloid profiles of the herb drugs were obtained without the need of complicated sample preparation to avoid potential damage or change of the active components. Seed tissues that were optimally sliced to a thickness of 10–20 μm from the crude and processed Strychnos Nux-Vomica seeds as well as various parts of tissue such as endosperm and epidermis were analyzed on MALDI target plate after the matrix was directly applied onto the tissue surface. The obtained alkaloid profiles provided valuable information for the differentiation of crude and processed Strychnos Nux-Vomica seeds and for the explanation of the significantly different toxicity. Experimental results indicated that the direct MALDI-TOFMS analysis allowed rapid screening of the alkaloid components in Strychnos Nux-Vomica seeds.
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Alkaloid profiling in crude and processed Strychnos Nux-Vomica seeds by matrix-assisted laser desorption/ionization-time of flight mass spectrometry
Journal of Pharmaceutical and Biomedical Analysis, 2007Co-Authors: Wei Wu, Chunfeng Qiao, Zhitao Liang, Hongxi Xu, Zhongzhen ZhaoAbstract:Abstract Direct analysis of alkaloids in the tissues of crude and processed Strychnos Nux-Vomica seeds by MALDI-TOFMS was described. The alkaloid profiles of the herb drugs were obtained without the need of complicated sample preparation to avoid potential damage or change of the active components. Seed tissues that were optimally sliced to a thickness of 10–20 μm from the crude and processed Strychnos Nux-Vomica seeds as well as various parts of tissue such as endosperm and epidermis were analyzed on MALDI target plate after the matrix was directly applied onto the tissue surface. The obtained alkaloid profiles provided valuable information for the differentiation of crude and processed Strychnos Nux-Vomica seeds and for the explanation of the significantly different toxicity. Experimental results indicated that the direct MALDI-TOFMS analysis allowed rapid screening of the alkaloid components in Strychnos Nux-Vomica seeds.
