Stylosanthes Guianensis

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Maria Lucia Carneiro Vieira - One of the best experts on this subject based on the ideXlab platform.

  • identification of Stylosanthes Guianensis varieties using molecular genetic analysis
    Aob Plants, 2012
    Co-Authors: M O Santosgarcia, Maria Lucia Carneiro Vieira, L Chiari, R M S Resende, C T Karia, Maria Imaculada Zucchi, Anete Pereira De Souza
    Abstract:

    Background and aims The botanical classification of Stylosanthes Guianensis is controversial, and few studies have used molecular markers to analyse this species. We used microsatellite markers to study the genetic diversity and population structure of S. Guianensis and compare our results with the current infraspecific botanical classification. Methodology A representative sample from the S. Guianensis Brazilian germplasm collection (150 accessions) was analysed using 20 microsatellite loci. A model-based Bayesian approach implemented in the software STRUCTURE was used to assign accessions into clusters. A dendrogram was constructed based on Roger’s genetic distances. Principal results The number of alleles per locus varied from 2 to 11, with an average of 4.7. The observed (HO) and expected (HE) heterozygosity values varied from 0 to 0.58 (mean of 0.18) and from 0.04 to 0.83 (mean of 0.55), respectively. Nine groups were assembled in STRUCTURE, and these groups were consistent with clusters inferred from the genetic distances and taxonomic varieties described for S. Guianensis. The GST among the nine groups was 0.46. Conclusions The low HO and the GST values observed are in agreement with the outcrossing rate (26 %) estimated for this species. The data indicate a high genetic diversity among and within the botanical varieties and suggest that microsatellite-based information can be combined with classical taxonomy to elucidate infraspecific levels.

  • microparticle bombardment of Stylosanthes Guianensis transformation parameters and expression of a methionine rich 2s albumin gene
    Plant Cell Tissue and Organ Culture, 2006
    Co-Authors: Vera Maria Quecini, C A De Oliveira, Anapaulanegreirosnunes Alves, Francisco J L Aragao, E L Rech, Elionor Rita Pereira De Almeida, E S Gander, Maria Lucia Carneiro Vieira
    Abstract:

    An effective protocol to generate stable transformants of the tropical forage legume Stylosanthes Guianensis (Aubl.) Sw. in a selection-free system was developed. Based on transient reporter gene expression, we have obtained transformation rates of 3.47% using 30-day-old calli as target, 1300 psi helium pressure, 12.5 cm microprojectile flight distance, 10–20 mm distance between macrocarrier membrane and stopping screen and 10–20 mm gap distance between the shock wave generator and the macrocarrier. These parameters were utilized to produce transgenic S. Guianensis plants expressing Be2S1 from Bertholletia excelsa that codes for a methionine-rich storage protein driven by a green-tissue specific promoter, Ats1 from Arabidopsis thaliana. Transgenic plants were identified by a PCR-based high-throughput screen in a selective agent-free system, employing pools of 20–50 regenerating shoots. The integration of the exogenous gene in the host genome was confirmed by Southern blot analysis of PCR-positive plants. The expression of the introduced gene was confirmed in leaf tissue of transgenic plants by Northern and Western blot analyses. Immunoblots of cellular fractions showed that BE2S1 expressed in Stylosanthes is mainly targeted to the vacuoles.

  • factors influencing electroporation mediated gene transfer to Stylosanthes Guianensis aubl sw protoplasts
    Genetics and Molecular Biology, 2002
    Co-Authors: Vera Maria Quecini, C A De Oliveira, Anapaulanegreirosnunes Alves, Maria Lucia Carneiro Vieira
    Abstract:

    Abstract In order to develop a high-efficiency and reproducible transformation protocol for Stylosanthes Guianensis weassessed the biological and physical parameters affecting plant electroporation protoplasts. Energy input, ascombinations of electric field strengths discharged by different capacitors, electroporation buffer and DNA form wereevaluated. Transformation efficiency was assayed in vivo as transient reporter gene expression, using theGFP-coding gene mgfp5 driven by a CaMV 35S constitutive promoter. Energy input and electric field strength had acritical influence on transgene expression with higher transformation levels being achieved with 250 V.cm -1 discharged by 900 and 1000 µF capacitors. Linear plasmid DNA, the absence of chloride and the presence ofcalcium ions also increased transient gene expression, albeit not significantly. Key words: directgenetransfer,planttransformation,foragelegume, Stylosanthes Guianensis .Received:February4,2000;accepted:March14,2002. Introduction

  • factors influencing electroporation mediated gene transfer to Stylosanthes Guianensis aubl sw protoplasts
    Genetics and Molecular Biology, 2002
    Co-Authors: Vera Maria Quecini, C A De Oliveira, Anapaulanegreirosnunes Alves, Maria Lucia Carneiro Vieira
    Abstract:

    In order to develop a high-efficiency and reproducible transformation protocol for Stylosanthes Guianensis we assessed the biological and physical parameters affecting plant electroporation protoplasts. Energy input, as combinations of electric field strengths discharged by different capacitors, electroporation buffer and DNA form were evaluated. Transformation efficiency was assayed in vivo as transient reporter gene expression, using the GFP-coding gene mgfp5 driven by a CaMV 35S constitutive promoter. Energy input and electric field strength had a critical influence on transgene expression with higher transformation levels being achieved with 250 V.cm-1 discharged by 900 and 1000 mF capacitors. Linear plasmid DNA, the absence of chloride and the presence of calcium ions also increased transient gene expression, albeit not significantly.

  • transient gene expression in electroporated intact tissues of Stylosanthes Guianensis aubl sw
    Scientia Agricola, 2001
    Co-Authors: Vera Maria Quecini, Maria Lucia Carneiro Vieira
    Abstract:

    A transformacao genetica atraves da eletroporacao de protoplastos foi estabelecida para especies vegetais comercialmente importantes. Neste trabalho, fontes de explante, intensidades de campo eletrico, solucoes de eletroporacao, configuracao da molecula de DNA e pre-tratamentos osmoticos foram avaliados para otimizar a expressao transiente do gene reporter em tecidos eletroporados de Stylosanthes Guianensis, uma leguminosa forrageira tropical. Taxas elevadas de transformacao foram obtidas empregando-se explantes cotiledonares e 250 V cm-1 de intensidade de campo eletrico. DNA plasmidial linear, solucao de eletroporacao livre de cloro e pre-tratamento osmotico com 1,6 mol L-1 de manitol favorecerem a expressao transiente do gene reporter, porem nao significativamente. A amplificacao por PCR especifica do transgene foi usada para demonstrar a ocorrencia de transformacao nos tecidos.

Vera Maria Quecini - One of the best experts on this subject based on the ideXlab platform.

  • microparticle bombardment of Stylosanthes Guianensis transformation parameters and expression of a methionine rich 2s albumin gene
    Plant Cell Tissue and Organ Culture, 2006
    Co-Authors: Vera Maria Quecini, C A De Oliveira, Anapaulanegreirosnunes Alves, Francisco J L Aragao, E L Rech, Elionor Rita Pereira De Almeida, E S Gander, Maria Lucia Carneiro Vieira
    Abstract:

    An effective protocol to generate stable transformants of the tropical forage legume Stylosanthes Guianensis (Aubl.) Sw. in a selection-free system was developed. Based on transient reporter gene expression, we have obtained transformation rates of 3.47% using 30-day-old calli as target, 1300 psi helium pressure, 12.5 cm microprojectile flight distance, 10–20 mm distance between macrocarrier membrane and stopping screen and 10–20 mm gap distance between the shock wave generator and the macrocarrier. These parameters were utilized to produce transgenic S. Guianensis plants expressing Be2S1 from Bertholletia excelsa that codes for a methionine-rich storage protein driven by a green-tissue specific promoter, Ats1 from Arabidopsis thaliana. Transgenic plants were identified by a PCR-based high-throughput screen in a selective agent-free system, employing pools of 20–50 regenerating shoots. The integration of the exogenous gene in the host genome was confirmed by Southern blot analysis of PCR-positive plants. The expression of the introduced gene was confirmed in leaf tissue of transgenic plants by Northern and Western blot analyses. Immunoblots of cellular fractions showed that BE2S1 expressed in Stylosanthes is mainly targeted to the vacuoles.

  • factors influencing electroporation mediated gene transfer to Stylosanthes Guianensis aubl sw protoplasts
    Genetics and Molecular Biology, 2002
    Co-Authors: Vera Maria Quecini, C A De Oliveira, Anapaulanegreirosnunes Alves, Maria Lucia Carneiro Vieira
    Abstract:

    Abstract In order to develop a high-efficiency and reproducible transformation protocol for Stylosanthes Guianensis weassessed the biological and physical parameters affecting plant electroporation protoplasts. Energy input, ascombinations of electric field strengths discharged by different capacitors, electroporation buffer and DNA form wereevaluated. Transformation efficiency was assayed in vivo as transient reporter gene expression, using theGFP-coding gene mgfp5 driven by a CaMV 35S constitutive promoter. Energy input and electric field strength had acritical influence on transgene expression with higher transformation levels being achieved with 250 V.cm -1 discharged by 900 and 1000 µF capacitors. Linear plasmid DNA, the absence of chloride and the presence ofcalcium ions also increased transient gene expression, albeit not significantly. Key words: directgenetransfer,planttransformation,foragelegume, Stylosanthes Guianensis .Received:February4,2000;accepted:March14,2002. Introduction

  • factors influencing electroporation mediated gene transfer to Stylosanthes Guianensis aubl sw protoplasts
    Genetics and Molecular Biology, 2002
    Co-Authors: Vera Maria Quecini, C A De Oliveira, Anapaulanegreirosnunes Alves, Maria Lucia Carneiro Vieira
    Abstract:

    In order to develop a high-efficiency and reproducible transformation protocol for Stylosanthes Guianensis we assessed the biological and physical parameters affecting plant electroporation protoplasts. Energy input, as combinations of electric field strengths discharged by different capacitors, electroporation buffer and DNA form were evaluated. Transformation efficiency was assayed in vivo as transient reporter gene expression, using the GFP-coding gene mgfp5 driven by a CaMV 35S constitutive promoter. Energy input and electric field strength had a critical influence on transgene expression with higher transformation levels being achieved with 250 V.cm-1 discharged by 900 and 1000 mF capacitors. Linear plasmid DNA, the absence of chloride and the presence of calcium ions also increased transient gene expression, albeit not significantly.

  • transient gene expression in electroporated intact tissues of Stylosanthes Guianensis aubl sw
    Scientia Agricola, 2001
    Co-Authors: Vera Maria Quecini, Maria Lucia Carneiro Vieira
    Abstract:

    A transformacao genetica atraves da eletroporacao de protoplastos foi estabelecida para especies vegetais comercialmente importantes. Neste trabalho, fontes de explante, intensidades de campo eletrico, solucoes de eletroporacao, configuracao da molecula de DNA e pre-tratamentos osmoticos foram avaliados para otimizar a expressao transiente do gene reporter em tecidos eletroporados de Stylosanthes Guianensis, uma leguminosa forrageira tropical. Taxas elevadas de transformacao foram obtidas empregando-se explantes cotiledonares e 250 V cm-1 de intensidade de campo eletrico. DNA plasmidial linear, solucao de eletroporacao livre de cloro e pre-tratamento osmotico com 1,6 mol L-1 de manitol favorecerem a expressao transiente do gene reporter, porem nao significativamente. A amplificacao por PCR especifica do transgene foi usada para demonstrar a ocorrencia de transformacao nos tecidos.

Guodao Liu - One of the best experts on this subject based on the ideXlab platform.

  • a root associated purple acid phosphatase sgpap23 mediates extracellular phytate p utilization in Stylosanthes Guianensis
    Plant Cell and Environment, 2018
    Co-Authors: Pandao Liu, Zefei Cai, Zhijian Chen, Xipeng Ding, Cuiyue Liang, Guodao Liu, Jiang Tian
    Abstract:

    As a major component of soil organic phosphorus (P), phytate-P is unavailable to plants unless hydrolysed by phytase to release inorganic phosphate. However, knowledge on natural variation in root-associated phytase activity and its underlying molecular mechanisms in plants remains fragmentary. In this study, variations in root internal and associated phytase activity were observed among 39 genotypes of Stylosanthes Guianensis (Stylo), which is well adapted to acid soils. Furthermore, TPRC2001-1, the genotype with the highest root-associated phytase activity, was more capable of utilizing extracellular phytate-P than Fine-stem, the genotype with the lowest root-associated phytase activity. After protein liquid chromatography-tandem mass spectrometry analysis, a purple acid phosphatase (PAP), SgPAP23, was identified and cloned from TPRC2001-1. SgPAP23 exhibited high activity against phytate-P and was mainly localized on the plasma membrane. Furthermore, SgPAP23 overexpression resulted in significant increases of root-associated phytase activity and thus facilitated extracellular phytate-P utilization in both bean (Phaseolus vulgaris) hairy roots and Arabidopsis thaliana. The results herein support the conclusion that SgPAP23 is a primary contributor to the superior extracellular phytate-P utilization in stylo and thus is used to develop cultivars with efficient extracellular phytate-P utilization.

  • development and characterization of expressed sequence tag derived simple sequence repeat markers in tropical forage legume Stylosanthes Guianensis aubl sw
    Molecular Breeding, 2015
    Co-Authors: Xipeng Ding, Guodao Liu, Qinglin Jia, Xiaoyan Luo, Long Zhang, Hanqing Cong, Changjun Bai
    Abstract:

    Most species of Stylosanthes (stylo) genus are important tropical pasture legumes with very versatile, widely adapted, and productive characteristics. These legumes are commercially used in various agricultural systems in many tropical and subtropical regions. However, the few molecular markers for the stylo species limit their genetic improvement. In this study, 36,558 expressed sequence tags (ESTs) have been de novo assembled using Illumina paired-end sequencing in Stylosanthes Guianensis (Aubl.) Sw. to develop simple sequence repeat (SSR) markers. We searched these ESTs for SSRs and identified 4115 SSR loci from 3643 ESTs (9.96 %). Dinucleotide and trinucleotide repeat motifs were the most abundant types (30.50 and 50.33 %, respectively), whereas tetranucleotide, pentanucleotide, and hexanucleotide motifs represented <10 % of all SSRs. The motif AG/CT was the most abundant, accounting for 21.7 % of all SSRs. Moreover, 2008 SSR markers were developed using 1873 SSR-containing unigenes. A total of 115 EST-SSR markers located in the coding region were amplified using polymerase chain reaction to detect 29 S. Guianensis accessions. Of these 115 markers, 96 produced reliable bands with expected sizes, and 81 markers were polymorphic, with 2–6 alleles among the 29 accessions. Analysis of the genetic diversity of all 29 accessions revealed similarity coefficients that ranged from 0.528 to 0.983. The EST-SSR markers developed in this study represent the first large-scale development of SSR markers for S. Guianensis. These SSR markers will provide a valuable resource for genetic diversity studies, cultivar fingerprinting, construction of genetic maps, identification of quantitative trait loci for important traits, and molecular marker-assisted selection breeding in stylo species.

  • malate synthesis and secretion mediated by a manganese enhanced malate dehydrogenase confers superior manganese tolerance in Stylosanthes Guianensis
    Plant Physiology, 2015
    Co-Authors: Zhijian Chen, Pandao Liu, Guodao Liu, Jiang Tian, Lili Sun, Hong Liao
    Abstract:

    Manganese (Mn) toxicity is a major constraint limiting plant growth on acidic soils. Superior Mn tolerance in Stylosanthes spp. has been well documented, but its molecular mechanisms remain largely unknown. In this study, superior Mn tolerance in Stylosanthes Guianensis was confirmed, as reflected by a high Mn toxicity threshold. Furthermore, genetic variation of Mn tolerance was evaluated using two S. Guianensis genotypes, which revealed that the Fine-stem genotype had higher Mn tolerance than the TPRC2001-1 genotype, as exhibited through less reduction in dry weight under excess Mn, and accompanied by lower internal Mn concentrations. Interestingly, Mn-stimulated increases in malate concentrations and exudation rates were observed only in the Fine-stem genotype. Proteomic analysis of Fine-stem roots revealed that S. Guianensis Malate Dehydrogenase1 (SgMDH1) accumulated in response to Mn toxicity. Western-blot and quantitative PCR analyses showed that Mn toxicity resulted in increased SgMDH1 accumulation only in Fine-stem roots, but not in TPRC2001-1. The function of SgMDH1-mediated malate synthesis was verified through in vitro biochemical analysis of SgMDH1 activities against oxaloacetate, as well as in vivo increased malate concentrations in yeast (Saccharomyces cerevisiae), soybean (Glycine max) hairy roots, and Arabidopsis (Arabidopsis thaliana) with SgMDH1 overexpression. Furthermore, SgMDH1 overexpression conferred Mn tolerance in Arabidopsis, which was accompanied by increased malate exudation and reduced plant Mn concentrations, suggesting that secreted malate could alleviate Mn toxicity in plants. Taken together, we conclude that the superior Mn tolerance of S. Guianensis is achieved by coordination of internal and external Mn detoxification through malate synthesis and exudation, which is regulated by SgMDH1 at both transcription and protein levels.

  • malate synthesis and secretion mediated by a manganese enhanced malate dehydrogenase confers superior manganese tolerance in Stylosanthes Guianensis 1
    2015
    Co-Authors: Zhijian Chen, Pandao Liu, Guodao Liu, Jiang Tian, Lili Sun, Hong Liao
    Abstract:

    Manganese (Mn) toxicity is a major constraint limiting plant growth on acidic soils. Superior Mn tolerance in Stylosanthes spp. has been well documented, but its molecular mechanisms remain largely unknown. In this study, superior Mn tolerance in Stylosanthes Guianensis was confirmed, as reflected by a high Mn toxicity threshold. Furthermore, genetic variation of Mn tolerance was evaluated using two S. Guianensis genotypes, which revealed that the Fine-stem genotype had higher Mn tolerance than the TPRC2001-1 genotype, as exhibited through less reduction in dry weight under excess Mn, and accompanied by lower internal Mn concentrations. Interestingly, Mn-stimulated increases in malate concentrations and exudation rates were observed only in the Fine-stem genotype. Proteomic analysis of Fine-stem roots revealed that S. Guianensis Malate Dehydrogenase1 (SgMDH1) accumulated in response to Mn toxicity. Western-blot and quantitative PCR analyses showed that Mn toxicity resulted in increased SgMDH1 accumulation only in Fine-stem roots, but not in TPRC2001-1. The function of SgMDH1-mediated malate synthesis was verified through in vitro biochemical analysis of SgMDH1 activities against oxaloacetate, as well as in vivo increased malate concentrations in yeast (Saccharomyces cerevisiae), soybean (Glycine max) hairy roots, and Arabidopsis (Arabidopsis thaliana) with SgMDH1 overexpression. Furthermore, SgMDH1 overexpression conferred Mn tolerance in Arabidopsis, which was accompanied by increased malate exudation and reduced plant Mn concentrations, suggesting that secreted malate could alleviate Mn toxicity in plants. Taken together, we conclude that the superior Mn tolerance of S. Guianensis is achieved by coordination of internal and external Mn detoxification through malate synthesis and exudation, which is regulated by SgMDH1 at both transcription and protein levels. Manganese (Mn) is an essential micronutrient for plant growth, and is a constitutive element in the Mn cluster of the oxygen-evolving complex in PSII, which is involved in the water-splitting process to oxidize

Zhijian Chen - One of the best experts on this subject based on the ideXlab platform.

  • a root associated purple acid phosphatase sgpap23 mediates extracellular phytate p utilization in Stylosanthes Guianensis
    Plant Cell and Environment, 2018
    Co-Authors: Pandao Liu, Zefei Cai, Zhijian Chen, Xipeng Ding, Cuiyue Liang, Guodao Liu, Jiang Tian
    Abstract:

    As a major component of soil organic phosphorus (P), phytate-P is unavailable to plants unless hydrolysed by phytase to release inorganic phosphate. However, knowledge on natural variation in root-associated phytase activity and its underlying molecular mechanisms in plants remains fragmentary. In this study, variations in root internal and associated phytase activity were observed among 39 genotypes of Stylosanthes Guianensis (Stylo), which is well adapted to acid soils. Furthermore, TPRC2001-1, the genotype with the highest root-associated phytase activity, was more capable of utilizing extracellular phytate-P than Fine-stem, the genotype with the lowest root-associated phytase activity. After protein liquid chromatography-tandem mass spectrometry analysis, a purple acid phosphatase (PAP), SgPAP23, was identified and cloned from TPRC2001-1. SgPAP23 exhibited high activity against phytate-P and was mainly localized on the plasma membrane. Furthermore, SgPAP23 overexpression resulted in significant increases of root-associated phytase activity and thus facilitated extracellular phytate-P utilization in both bean (Phaseolus vulgaris) hairy roots and Arabidopsis thaliana. The results herein support the conclusion that SgPAP23 is a primary contributor to the superior extracellular phytate-P utilization in stylo and thus is used to develop cultivars with efficient extracellular phytate-P utilization.

  • malate synthesis and secretion mediated by a manganese enhanced malate dehydrogenase confers superior manganese tolerance in Stylosanthes Guianensis
    Plant Physiology, 2015
    Co-Authors: Zhijian Chen, Pandao Liu, Guodao Liu, Jiang Tian, Lili Sun, Hong Liao
    Abstract:

    Manganese (Mn) toxicity is a major constraint limiting plant growth on acidic soils. Superior Mn tolerance in Stylosanthes spp. has been well documented, but its molecular mechanisms remain largely unknown. In this study, superior Mn tolerance in Stylosanthes Guianensis was confirmed, as reflected by a high Mn toxicity threshold. Furthermore, genetic variation of Mn tolerance was evaluated using two S. Guianensis genotypes, which revealed that the Fine-stem genotype had higher Mn tolerance than the TPRC2001-1 genotype, as exhibited through less reduction in dry weight under excess Mn, and accompanied by lower internal Mn concentrations. Interestingly, Mn-stimulated increases in malate concentrations and exudation rates were observed only in the Fine-stem genotype. Proteomic analysis of Fine-stem roots revealed that S. Guianensis Malate Dehydrogenase1 (SgMDH1) accumulated in response to Mn toxicity. Western-blot and quantitative PCR analyses showed that Mn toxicity resulted in increased SgMDH1 accumulation only in Fine-stem roots, but not in TPRC2001-1. The function of SgMDH1-mediated malate synthesis was verified through in vitro biochemical analysis of SgMDH1 activities against oxaloacetate, as well as in vivo increased malate concentrations in yeast (Saccharomyces cerevisiae), soybean (Glycine max) hairy roots, and Arabidopsis (Arabidopsis thaliana) with SgMDH1 overexpression. Furthermore, SgMDH1 overexpression conferred Mn tolerance in Arabidopsis, which was accompanied by increased malate exudation and reduced plant Mn concentrations, suggesting that secreted malate could alleviate Mn toxicity in plants. Taken together, we conclude that the superior Mn tolerance of S. Guianensis is achieved by coordination of internal and external Mn detoxification through malate synthesis and exudation, which is regulated by SgMDH1 at both transcription and protein levels.

  • malate synthesis and secretion mediated by a manganese enhanced malate dehydrogenase confers superior manganese tolerance in Stylosanthes Guianensis 1
    2015
    Co-Authors: Zhijian Chen, Pandao Liu, Guodao Liu, Jiang Tian, Lili Sun, Hong Liao
    Abstract:

    Manganese (Mn) toxicity is a major constraint limiting plant growth on acidic soils. Superior Mn tolerance in Stylosanthes spp. has been well documented, but its molecular mechanisms remain largely unknown. In this study, superior Mn tolerance in Stylosanthes Guianensis was confirmed, as reflected by a high Mn toxicity threshold. Furthermore, genetic variation of Mn tolerance was evaluated using two S. Guianensis genotypes, which revealed that the Fine-stem genotype had higher Mn tolerance than the TPRC2001-1 genotype, as exhibited through less reduction in dry weight under excess Mn, and accompanied by lower internal Mn concentrations. Interestingly, Mn-stimulated increases in malate concentrations and exudation rates were observed only in the Fine-stem genotype. Proteomic analysis of Fine-stem roots revealed that S. Guianensis Malate Dehydrogenase1 (SgMDH1) accumulated in response to Mn toxicity. Western-blot and quantitative PCR analyses showed that Mn toxicity resulted in increased SgMDH1 accumulation only in Fine-stem roots, but not in TPRC2001-1. The function of SgMDH1-mediated malate synthesis was verified through in vitro biochemical analysis of SgMDH1 activities against oxaloacetate, as well as in vivo increased malate concentrations in yeast (Saccharomyces cerevisiae), soybean (Glycine max) hairy roots, and Arabidopsis (Arabidopsis thaliana) with SgMDH1 overexpression. Furthermore, SgMDH1 overexpression conferred Mn tolerance in Arabidopsis, which was accompanied by increased malate exudation and reduced plant Mn concentrations, suggesting that secreted malate could alleviate Mn toxicity in plants. Taken together, we conclude that the superior Mn tolerance of S. Guianensis is achieved by coordination of internal and external Mn detoxification through malate synthesis and exudation, which is regulated by SgMDH1 at both transcription and protein levels. Manganese (Mn) is an essential micronutrient for plant growth, and is a constitutive element in the Mn cluster of the oxygen-evolving complex in PSII, which is involved in the water-splitting process to oxidize

Jiang Tian - One of the best experts on this subject based on the ideXlab platform.

  • a root associated purple acid phosphatase sgpap23 mediates extracellular phytate p utilization in Stylosanthes Guianensis
    Plant Cell and Environment, 2018
    Co-Authors: Pandao Liu, Zefei Cai, Zhijian Chen, Xipeng Ding, Cuiyue Liang, Guodao Liu, Jiang Tian
    Abstract:

    As a major component of soil organic phosphorus (P), phytate-P is unavailable to plants unless hydrolysed by phytase to release inorganic phosphate. However, knowledge on natural variation in root-associated phytase activity and its underlying molecular mechanisms in plants remains fragmentary. In this study, variations in root internal and associated phytase activity were observed among 39 genotypes of Stylosanthes Guianensis (Stylo), which is well adapted to acid soils. Furthermore, TPRC2001-1, the genotype with the highest root-associated phytase activity, was more capable of utilizing extracellular phytate-P than Fine-stem, the genotype with the lowest root-associated phytase activity. After protein liquid chromatography-tandem mass spectrometry analysis, a purple acid phosphatase (PAP), SgPAP23, was identified and cloned from TPRC2001-1. SgPAP23 exhibited high activity against phytate-P and was mainly localized on the plasma membrane. Furthermore, SgPAP23 overexpression resulted in significant increases of root-associated phytase activity and thus facilitated extracellular phytate-P utilization in both bean (Phaseolus vulgaris) hairy roots and Arabidopsis thaliana. The results herein support the conclusion that SgPAP23 is a primary contributor to the superior extracellular phytate-P utilization in stylo and thus is used to develop cultivars with efficient extracellular phytate-P utilization.

  • malate synthesis and secretion mediated by a manganese enhanced malate dehydrogenase confers superior manganese tolerance in Stylosanthes Guianensis
    Plant Physiology, 2015
    Co-Authors: Zhijian Chen, Pandao Liu, Guodao Liu, Jiang Tian, Lili Sun, Hong Liao
    Abstract:

    Manganese (Mn) toxicity is a major constraint limiting plant growth on acidic soils. Superior Mn tolerance in Stylosanthes spp. has been well documented, but its molecular mechanisms remain largely unknown. In this study, superior Mn tolerance in Stylosanthes Guianensis was confirmed, as reflected by a high Mn toxicity threshold. Furthermore, genetic variation of Mn tolerance was evaluated using two S. Guianensis genotypes, which revealed that the Fine-stem genotype had higher Mn tolerance than the TPRC2001-1 genotype, as exhibited through less reduction in dry weight under excess Mn, and accompanied by lower internal Mn concentrations. Interestingly, Mn-stimulated increases in malate concentrations and exudation rates were observed only in the Fine-stem genotype. Proteomic analysis of Fine-stem roots revealed that S. Guianensis Malate Dehydrogenase1 (SgMDH1) accumulated in response to Mn toxicity. Western-blot and quantitative PCR analyses showed that Mn toxicity resulted in increased SgMDH1 accumulation only in Fine-stem roots, but not in TPRC2001-1. The function of SgMDH1-mediated malate synthesis was verified through in vitro biochemical analysis of SgMDH1 activities against oxaloacetate, as well as in vivo increased malate concentrations in yeast (Saccharomyces cerevisiae), soybean (Glycine max) hairy roots, and Arabidopsis (Arabidopsis thaliana) with SgMDH1 overexpression. Furthermore, SgMDH1 overexpression conferred Mn tolerance in Arabidopsis, which was accompanied by increased malate exudation and reduced plant Mn concentrations, suggesting that secreted malate could alleviate Mn toxicity in plants. Taken together, we conclude that the superior Mn tolerance of S. Guianensis is achieved by coordination of internal and external Mn detoxification through malate synthesis and exudation, which is regulated by SgMDH1 at both transcription and protein levels.

  • malate synthesis and secretion mediated by a manganese enhanced malate dehydrogenase confers superior manganese tolerance in Stylosanthes Guianensis 1
    2015
    Co-Authors: Zhijian Chen, Pandao Liu, Guodao Liu, Jiang Tian, Lili Sun, Hong Liao
    Abstract:

    Manganese (Mn) toxicity is a major constraint limiting plant growth on acidic soils. Superior Mn tolerance in Stylosanthes spp. has been well documented, but its molecular mechanisms remain largely unknown. In this study, superior Mn tolerance in Stylosanthes Guianensis was confirmed, as reflected by a high Mn toxicity threshold. Furthermore, genetic variation of Mn tolerance was evaluated using two S. Guianensis genotypes, which revealed that the Fine-stem genotype had higher Mn tolerance than the TPRC2001-1 genotype, as exhibited through less reduction in dry weight under excess Mn, and accompanied by lower internal Mn concentrations. Interestingly, Mn-stimulated increases in malate concentrations and exudation rates were observed only in the Fine-stem genotype. Proteomic analysis of Fine-stem roots revealed that S. Guianensis Malate Dehydrogenase1 (SgMDH1) accumulated in response to Mn toxicity. Western-blot and quantitative PCR analyses showed that Mn toxicity resulted in increased SgMDH1 accumulation only in Fine-stem roots, but not in TPRC2001-1. The function of SgMDH1-mediated malate synthesis was verified through in vitro biochemical analysis of SgMDH1 activities against oxaloacetate, as well as in vivo increased malate concentrations in yeast (Saccharomyces cerevisiae), soybean (Glycine max) hairy roots, and Arabidopsis (Arabidopsis thaliana) with SgMDH1 overexpression. Furthermore, SgMDH1 overexpression conferred Mn tolerance in Arabidopsis, which was accompanied by increased malate exudation and reduced plant Mn concentrations, suggesting that secreted malate could alleviate Mn toxicity in plants. Taken together, we conclude that the superior Mn tolerance of S. Guianensis is achieved by coordination of internal and external Mn detoxification through malate synthesis and exudation, which is regulated by SgMDH1 at both transcription and protein levels. Manganese (Mn) is an essential micronutrient for plant growth, and is a constitutive element in the Mn cluster of the oxygen-evolving complex in PSII, which is involved in the water-splitting process to oxidize

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