The Experts below are selected from a list of 315 Experts worldwide ranked by ideXlab platform
Muqing Zhang - One of the best experts on this subject based on the ideXlab platform.
-
photosynthetic characterization and expression profiles of Sugarcane infected by Sugarcane Mosaic Virus scmv
Photosynthesis Research, 2020Co-Authors: Sehrish Akbar, Kai Yu, Miaohong Ruan, Charles A Powell, Baoshan Chen, Muqing ZhangAbstract:: Sugarcane Mosaic Virus (SCMV), belonging to genus PotyVirus, family Potyviridae, is a severe pathogen of several agricultural important crops, mainly Sugarcane. Due to complex nature of Sugarcane, the effect of SCMV pathogenicity on Sugarcane photosynthetic systems remains to be explored. In this study, we investigated the alterations occurring in the photosynthetic system in the Sugarcane genotypes at the cytopathological, physiological and biological, transcriptome and proteome level. We generated the transcriptome assembly of two genotypes (susceptible Badila and resistant B-48) using Saccharum spontaneum L. as a reference genome. RNA-sequencing data revealed the significant upregulation of NAD(P)H, RubisCO, oxygen-evolving complex, chlorophyll a and b binding protein, Psb protein family, PSI reaction center subunit II, and IVgenes in B-48, as compared to its counterparts. Upregulated genes in B-48 are associated with various processes such as stability and assembly of photosystem, protection against photoinhibition and antiviral defense. The expression pattern of differentially abundant genes were further verified at the proteomics level. Overall, differentially expressed genes/proteins (DEGs/DEPs) showed the consistency of expression at both transcriptome and proteome level in B-48 genotype. Comprehensively, these data supported the efficiency of B-48 genotype under Virus infection conditions and provided a better understanding of the expression pattern of photosynthesis-related genes in Sugarcane.
-
field performance of transgenic Sugarcane lines resistant to Sugarcane Mosaic Virus
Frontiers in Plant Science, 2017Co-Authors: Miaohong Ruan, Baoshan Chen, Muqing Zhang, Chuanyu Yang, Rukai ChenAbstract:Sugarcane Mosaic disease is mainly caused by the Sugarcane Mosaic Virus (SCMV), which can significantly reduce stalk yield and sucrose content of Sugarcane in the field. Coat protein mediated protection (CPMP) is an effective strategy to improve Virus resistance. A two-year field study was conducted to compare five independent transgenic Sugarcane lines carrying the SCMV-CP gene (i.e., B2, B36, B38, B48, and B51) with the wild-type parental clone Badila (WT). Agronomic performance, resistance to SCMV infection, and transgene stability were evaluated and compared with the wild-type parental clone Badila (WT) at four experimental locations in China across two successive seasons, i.e. plant cane (PC) and 1st ratoon cane (1R). All transgenic lines derived from Badila had significantly greater tons of cane per hectare (TCH) and tons of sucrose per hectare (TSH) as well as lower SCMV disease incidence than those from Badila in the PC and 1R crops. The transgenic line B48 was highly resistant to SCMV with less than 3% incidence of infection. The recovery phenotype of transgenic line B36 was infected soon after Virus inoculation, but the subsequent leaves showed no symptoms of infection. Most control plants developed symptoms that persisted and spread throughout the plant with more than 50% incidence. B48 recorded an average of 102.72 t/ha, which was 67.2% more than that for Badila. The expression of the transgene was stable over many generations with vegetative propagation. These results show that SCMV-resistant transgenic lines derived from Badila can provide resistant germplasm for Sugarcane breeding and can also be used to study Virus resistance mechanisms. This is the first report on the development and field performance of transgenic Sugarcane plants that are resistant to SCMV infection in China.
Thomas Lubberstedt - One of the best experts on this subject based on the ideXlab platform.
-
Molecular analysis of Sugarcane Mosaic Virus resistance in maize
2020Co-Authors: Christina Ingvardsen, Y. King, Mingliang Xu, Thomas LubberstedtAbstract:The PotyVirus Sugarcane Mosaic Virus (SCMV) is an important pathogen of maize (Zea mays L.), causing chlorosis, stunting and serious yield loss in susceptible cultivars. Three major resistance genes confer resistance to SCMV, one gene located on chromosome 3 and two genes closely linked on chromosome 6. However, the molecular mechanisms underlying the establishment and progression of SCMV infection in maize are poorly understood. A near-isogenic line, F7R, carrying the resistance regions from chromosome 3 (Scmv2) and 6 (Scmvla and 1b) in a susceptible background was developed. Based on F7R, nine isogenic genotypes segregating in these two regions were developed. Testing the nine genotypes for response to SCMV infection showed that the Scmvl and Scmv2 locus interact epistatically and that one resistance region alone was not sufficient for complete resistance against SCMV. When the resistant allele is fixed either at the Scmvl or Scmv2 loci, the susceptible homozygote at the other locus is easily distinguishable from the genotypes carrying one or two resistance alleles. Based on these findings we are currently in the process of map-based gene isolation using large F 2 populations for each of both genome regions. A BAC library has been constructed from the resistance gene donor of F7R, inbred FAP1360A, for physical mapping of the Scmvl and Scmv2 loci and isolation of the respective genes.
-
an atypical thioredoxin imparts early resistance to Sugarcane Mosaic Virus in maize
Molecular Plant, 2017Co-Authors: Yangqing Gong, Thomas Lubberstedt, Lu Jiang, Qin Yang, Jianrong Ye, Jianyu Wu, Mingliang XuAbstract:Abstract Sugarcane Mosaic Virus (SCMV) causes substantial losses of grain yield and forage biomass in susceptible maize worldwide. A major quantitative trait locus, Scmv1 , has been identified to impart strong resistance to SCMV at the early infection stage. Here, we demonstrate that ZmTrxh , encoding an atypical h-type thioredoxin, is the causal gene at Scmv1 , and that its transcript abundance correlated strongly with maize resistance to SCMV. ZmTrxh alleles, whether they are resistant or susceptible, share the identical coding/proximal promoter regions, but vary in the upstream regulatory regions. ZmTrxh lacks two canonical cysteines in the thioredoxin active-site motif and exists uniquely in the maize genome. Because of this, ZmTrxh is unable to reduce disulfide bridges but possesses a strong molecular chaperone-like activity. ZmTrxh is dispersed in maize cytoplasm to suppress SCMV viral RNA accumulation. Moreover, ZmTrxh -mediated maize resistance to SCMV showed no obvious correlation with the salicylic acid- and jasmonic acid-related defense signaling pathways. Taken together, our results indicate that ZmTrxh exhibits a distinct defense profile in maize resistance to SCMV, differing from previously characterized dominant or recessive potyVirus resistance genes.
-
the pic19 nbs lrr gene family members are closely linked to scmv1 but not involved in maize resistance to Sugarcane Mosaic Virus
Genome, 2008Co-Authors: Lu Jiangl Jiang, Christina Ingvardsen, Thomas Lubberstedt, Mingliang Xum L XuAbstract:Sugarcane Mosaic Virus (SCMV) is the causal pathogen for a severe Mosaic Virus disease of maize worldwide. In our previous research, the maize resistance gene analog (RGA) Pic19 and its three cogna...
-
analysis of Sugarcane Mosaic Virus resistance in maize in an isogenic dihybrid crossing scheme and implications for breeding potyVirus resistant maize hybrids
Genome, 2006Co-Authors: Yongzhong Xingy Xing, Christina Ingvardsen, Raphael Salomonr Salomon, Thomas LubberstedtAbstract:The gene action of 2 Sugarcane Mosaic Virus (SCMV) resistance loci in maize, Scmv1 and Scmv2, was evaluated for potyVirus resistance in an isogenic background. All 4 homozygous and 5 heterozygous isogenic genotypes were produced for introgressions of the resistant donor (FAP1360A) alleles at both loci into the susceptible parent (F7) genetic background using simple sequence repeat markers. For SCMV and maize dwarf Mosaic Virus (MDMV), Virus symptoms appeared rapidly in the 3 homozygous genotypes, with susceptibility alleles fixed at 1 or both loci. Although the 9 isogenic genotypes revealed a high level of resistance to Zea Mosaic Virus (ZeMV), the same 3 homozygous genotypes were only partially resistant. This indicates that 1 resistance gene alone is not sufficient for complete resistance against SCMV, MDMV, and ZeMV. Scmv1 showed strong early and complete dominant gene action to SCMV, but it gradually became partially dominant. Scmv2 was not detected at the beginning, showing dominant gene action initi...
-
development of rga caps markers and genetic mapping of candidate genes for Sugarcane Mosaic Virus resistance in maize
Theoretical and Applied Genetics, 2002Co-Authors: Marcel Quint, Albrecht E Melchinger, Mingliang Xu, Renata Mihaljevic, C M Dussle, Thomas LubberstedtAbstract:Three previously published resistance gene analogues (RGAs), pic13, pic21 and pic19, were mapped in relation to Sugarcane Mosaic Virus (SCMV) resistance genes (Scmv1, Scmv2) in maize. We cloned these RGAs from six inbreds including three SCMV-resistant lines (D21, D32, FAP1360A) and three SCMV-susceptible lines (D145, D408, F7). Pairwise sequence alignments among the six inbreds revealed a frequency of one single nucleotide polymorphism (SNP) per 33 bp for the three RGAs, indicating a high degree of polymorphism and a high probability of success in converting RGAs into codominant cleaved amplified polymorphic sequence (CAPS) markers compared to other sequences. SNPs were used to develop CAPS markers for mapping of the three RGAs in relation to Scmv1 (chromosome 6) and Scmv2 (chromosome 3), and for pedigree analyses of resistant inbred lines. By genetic mapping pic21 was shown to be different from Scmv2, whereas pic19 and pic13 are still candidates for Scmv1 and Scmv2, respectively, due to genetic mapping and consistent restriction patterns of ancestral lines.
J. L. Dale - One of the best experts on this subject based on the ideXlab platform.
-
Transient expression of the coat protein of Sugarcane Mosaic Virus in Sugarcane protoplasts and expression inEscherichia coli
Archives of Virology, 1992Co-Authors: G. R. Smith, D. D. Shukla, R. Ford, M. J. Frenkel, J. L. DaleAbstract:The coat protein (CP) of strain SC of Sugarcane Mosaic Virus (SCMV-SC) was expressed transiently in Sugarcane protoplasts after electroporation with one of two plasmids encoding the CP gene. The CP gene was fused with either the cauliflower Mosaic Virus 35S promoter or the synthetic monocotyledon promoter “Emu”. The coat protein gene was also inducibly expressed in Escherichia coli when fused to the trc promoter. The protein expressed in both systems had the same electrophoretic mobility and antigenic specificity as purified SCMV-SC coat protein. Transient expression of the 35S-CP gene in protoplasts could only be demonstrated in Western blots developed with the chemiluminescence enzyme substrate luminol.
-
transient expression of the coat protein of Sugarcane Mosaic Virus in Sugarcane protoplasts and expression in escherichia coli
Archives of Virology, 1992Co-Authors: G. R. Smith, D. D. Shukla, R E Ford, M. J. Frenkel, J. L. DaleAbstract:The coat protein (CP) of strain SC of Sugarcane Mosaic Virus (SCMV-SC) was expressed transiently in Sugarcane protoplasts after electroporation with one of two plasmids encoding the CP gene. The CP gene was fused with either the cauliflower Mosaic Virus 35S promoter or the synthetic monocotyledon promoter “Emu”. The coat protein gene was also inducibly expressed inEscherichia coli when fused to the trc promoter. The protein expressed in both systems had the same electrophoretic mobility and antigenic specificity as purified SCMV-SC coat protein. Transient expression of the 35S-CP gene in protoplasts could only be demonstrated in Western blots developed with the chemiluminescence enzyme substrate luminol.
Liuji Wu - One of the best experts on this subject based on the ideXlab platform.
-
phosphoproteomic analysis of the resistant and susceptible genotypes of maize infected with Sugarcane Mosaic Virus
Amino Acids, 2015Co-Authors: Liuji Wu, Shunxi Wang, Yanhui Chen, Jianyu Wu, Rui Wang, Liancheng Wu, Huimin Zhang, Xiuli HuAbstract:Protein phosphorylation plays a pivotal role in the regulation of many cellular events. No information is yet available, however, on protein phosphorylation in plants in response to Virus infection. In this study, we characterized phosphoproteomes of resistant and susceptible genotypes of maize (Zea mays L.) in response to Sugarcane Mosaic Virus (SCMV) infection. Based on isotope tags for relative and absolute quantification technology, TiO2 enrichment method and LC–MS/MS analysis, we identified 65 and 59 phosphoproteins respectively, whose phosphorylation level regulated significantly in susceptible and resistant plants. Some identified phosphoproteins were shared by both genotypes, suggesting a partial overlapping of the responsive pathways to Virus infection. While several phosphoproteins are well-known pathogen response phosphoproteins, Virus infection differentially regulates most other phosphoproteins, which has not been reported in literature. Changes in protein phosphorylation status indicated that response to SCMV infection encompass a reformatting of major cellular processes. Our data provide new valuable insights into plant-Virus interactions.
-
Sugarcane Mosaic Virus long history but still a threat to industry
Crop Protection, 2012Co-Authors: Liuji Wu, Xiaofeng Zu, Shunxi Wang, Yanhui ChenAbstract:Abstract Sugarcane Mosaic Virus (SCMV) infects maize, sorghum, Sugarcane and other poaceous species throughout the world. SCMV is an important Virus pathogen, especially in European and Chinese maize production, causing serious losses in grain and forage yields in susceptible cultivars. Like other potyViruses, SCMV is a positive-sense single-stranded RNA Virus with a genome size of approximately 10 kb in length. SCMV is naturally transmitted by aphids in a non-persistent manner. Control of the aphid vectors is not effective because of the non-persistent mode of Virus transmission. Therefore, cultivation of resistant maize varieties is the preferred way to control SCMV infections. The high incidence of co-infection and the occurrence of new strains or genome variations indicate that SCMV will continue to be a threat to industry. Aspects concerning Virus structure and genome organization, geographic distribution, diagnosis and strain characterization, and genetic variation are reviewed. Special emphasis is placed on the control of SCMV disease.
-
Sugarcane Mosaic Virus – Long history but still a threat to industry
Crop Protection, 2012Co-Authors: Liuji Wu, Xiaofeng Zu, Shunxi Wang, Yanhui ChenAbstract:Abstract Sugarcane Mosaic Virus (SCMV) infects maize, sorghum, Sugarcane and other poaceous species throughout the world. SCMV is an important Virus pathogen, especially in European and Chinese maize production, causing serious losses in grain and forage yields in susceptible cultivars. Like other potyViruses, SCMV is a positive-sense single-stranded RNA Virus with a genome size of approximately 10 kb in length. SCMV is naturally transmitted by aphids in a non-persistent manner. Control of the aphid vectors is not effective because of the non-persistent mode of Virus transmission. Therefore, cultivation of resistant maize varieties is the preferred way to control SCMV infections. The high incidence of co-infection and the occurrence of new strains or genome variations indicate that SCMV will continue to be a threat to industry. Aspects concerning Virus structure and genome organization, geographic distribution, diagnosis and strain characterization, and genetic variation are reviewed. Special emphasis is placed on the control of SCMV disease.
Miaohong Ruan - One of the best experts on this subject based on the ideXlab platform.
-
photosynthetic characterization and expression profiles of Sugarcane infected by Sugarcane Mosaic Virus scmv
Photosynthesis Research, 2020Co-Authors: Sehrish Akbar, Kai Yu, Miaohong Ruan, Charles A Powell, Baoshan Chen, Muqing ZhangAbstract:: Sugarcane Mosaic Virus (SCMV), belonging to genus PotyVirus, family Potyviridae, is a severe pathogen of several agricultural important crops, mainly Sugarcane. Due to complex nature of Sugarcane, the effect of SCMV pathogenicity on Sugarcane photosynthetic systems remains to be explored. In this study, we investigated the alterations occurring in the photosynthetic system in the Sugarcane genotypes at the cytopathological, physiological and biological, transcriptome and proteome level. We generated the transcriptome assembly of two genotypes (susceptible Badila and resistant B-48) using Saccharum spontaneum L. as a reference genome. RNA-sequencing data revealed the significant upregulation of NAD(P)H, RubisCO, oxygen-evolving complex, chlorophyll a and b binding protein, Psb protein family, PSI reaction center subunit II, and IVgenes in B-48, as compared to its counterparts. Upregulated genes in B-48 are associated with various processes such as stability and assembly of photosystem, protection against photoinhibition and antiviral defense. The expression pattern of differentially abundant genes were further verified at the proteomics level. Overall, differentially expressed genes/proteins (DEGs/DEPs) showed the consistency of expression at both transcriptome and proteome level in B-48 genotype. Comprehensively, these data supported the efficiency of B-48 genotype under Virus infection conditions and provided a better understanding of the expression pattern of photosynthesis-related genes in Sugarcane.
-
field performance of transgenic Sugarcane lines resistant to Sugarcane Mosaic Virus
Frontiers in Plant Science, 2017Co-Authors: Miaohong Ruan, Baoshan Chen, Muqing Zhang, Chuanyu Yang, Rukai ChenAbstract:Sugarcane Mosaic disease is mainly caused by the Sugarcane Mosaic Virus (SCMV), which can significantly reduce stalk yield and sucrose content of Sugarcane in the field. Coat protein mediated protection (CPMP) is an effective strategy to improve Virus resistance. A two-year field study was conducted to compare five independent transgenic Sugarcane lines carrying the SCMV-CP gene (i.e., B2, B36, B38, B48, and B51) with the wild-type parental clone Badila (WT). Agronomic performance, resistance to SCMV infection, and transgene stability were evaluated and compared with the wild-type parental clone Badila (WT) at four experimental locations in China across two successive seasons, i.e. plant cane (PC) and 1st ratoon cane (1R). All transgenic lines derived from Badila had significantly greater tons of cane per hectare (TCH) and tons of sucrose per hectare (TSH) as well as lower SCMV disease incidence than those from Badila in the PC and 1R crops. The transgenic line B48 was highly resistant to SCMV with less than 3% incidence of infection. The recovery phenotype of transgenic line B36 was infected soon after Virus inoculation, but the subsequent leaves showed no symptoms of infection. Most control plants developed symptoms that persisted and spread throughout the plant with more than 50% incidence. B48 recorded an average of 102.72 t/ha, which was 67.2% more than that for Badila. The expression of the transgene was stable over many generations with vegetative propagation. These results show that SCMV-resistant transgenic lines derived from Badila can provide resistant germplasm for Sugarcane breeding and can also be used to study Virus resistance mechanisms. This is the first report on the development and field performance of transgenic Sugarcane plants that are resistant to SCMV infection in China.
