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Wei Ding - One of the best experts on this subject based on the ideXlab platform.

  • Acaricidal Mechanism of Scopoletin Against Tetranychus cinnabarinus.
    Frontiers in Physiology, 2019
    Co-Authors: Hong Zhou, Yongqiang Zhang, Wei Ding
    Abstract:

    : Scopoletin is a promising acaricidal botanical natural compound against Tetranychus cinnabarinus, and its acaricidal mechanism maybe involve calcium overload according to our previous study. To seek potential candidate target genes of calcium overload induced by scopoletin in T. cinnabarinus, RNA-seq was utilized to detect changes in transcription levels. 24 and 48 h after treatment, 70 and 102 differentially expressed genes were obtained, respectively. Target genes included 3 signal transduction genes, 4 cell apoptosis genes, 4 energy metabolism genes, and 2 transcription factor genes. The role of 3 calcium signaling pathway-related genes, namely, G-protein-coupled neuropeptide receptor, Bcl-2 protein and guanylate kinase (designated TcGPCR, TcBAG, and TcGUK, respectively) in the calcium overload were investigated in this study. RT-qPCR detection showed that scopoletin treatment upregulated the expression level of TcGPCR and downregulated the expression level of TcBAG and TcGUK. The result of RNAi indicated that downregulation of TcGPCR decreased susceptibility to scopoletin, and downregulation of TcBAG and TcGUK enhanced susceptibility to scopoletin. Functional expression in Chinese hamster ovary cells showed that scopoletin induced a significant increase in intracellular free calcium [Ca2+]i levels by activating TcGPCR. These results demonstrated that the acaricidal mechanism of scopoletin was via disrupting intracellular Ca2+ homeostasis and calcium signaling pathway mediated by GPCR, BAG, and GUK.

  • Silencing Chitinase Genes Increases Susceptibility of Tetranychus cinnabarinus (Boisduval) to Scopoletin.
    BioMed Research International, 2017
    Co-Authors: Hong Zhou, Yongqiang Zhang, Dan Wang, Wei Ding
    Abstract:

    The carmine spider mite Tetranychus cinnabarinus is a major pest of crop and vegetable plants worldwide. Previous studies have shown that scopoletin is a promising acaricidal compound against Tetranychus cinnabarinus. However, the acaricidal mechanism of scopoletin remains unclear. In the present study, 12 full-length cDNAs of chitinase (CHIT) genes from Tetranychus cinnabarinus (designated TcCHITs) were cloned and characterized. Although TcCHITs were expressed throughout all life stages, their expression levels were significantly upregulated during the larval and nymphal stages. TcCHITs were downregulated 24 h after treatment with scopoletin and upregulated 24 h after treatment with diflubenzuron (DFB, a chitin synthesis inhibitor). Feeding double-stranded RNA effectively silenced TcCHIT transcription in Tetranychus cinnabarinus, thus increasing its susceptibility to scopoletin but reducing that to DFB. Meanwhile, TcCHIT silencing in larvae and adult resulted in an extremely low molting rate (7.3%) and high mortality rate (53.3%), respectively, compared with those in the control group. CHIT genes are closely related to arthropod survival, molting, and development in Tetranychus cinnabarinus, suggesting that acaricidal mechanisms of scopoletin and DFB may occur by inhibition and activation of CHIT gene expression, respectively. TcCHIT constitutes a possible target of scopoletin and DFB in Tetranychus cinnabarinus.

  • Acaricide resistance of Tetranychus cinnabarinus (Acari: Tetraychidae) from mulberry plantations in southwest China
    International Journal of Acarology, 2013
    Co-Authors: Luo Yanjie, Yongguang Liu, Jiang Xiujun, Chai Jianping, Xie Daoyan, Da Aisi, Huang Ping, Wei Ding
    Abstract:

    The present study is the first to investigate resistance levels of Tetranychus cinnabarinus (Boiduval) in mulberry in southwest China. The toxicity and resistance of seven common acaricides, including dichlorvos, phoxim, methomyl, pyridaben, propargite, abamectin and chlorfenapyr, were assayed for seven mulberry field strains of mites along with a laboratory susceptible strain (SS). The results indicated that most field strains exhibited low to moderate levels of resistance. Mengzi and Xiangyun had moderate resistance to phoxim (RR = 31.45 and 26.22, respectively). The Qiaojia, Luliang, Mengzi and Heqing strains showed moderate resistance to chlorfenapyr (RR = 14.98–22.52). Correlation analysis of resistance ratio in seven acaricides indicated that methomyl and abamectin were negatively correlated. The results provide information for acaricide rotations and resistance management of spider mite in mulberry plantations.

  • Action mechanism and biological activity of celangulin to Tetranychus cinnabarinus (Acari: Tetranychidae)
    Systematic & Applied Acarology, 2004
    Co-Authors: Wei Ding, Zhimo Zhao, Huiying Tao, Jin-jun Wang
    Abstract:

    Abstract The insecticidal activity of 2% Celangulin E.C against some populations of Tetranychus cinnabarinus, including a susceptible strain (SS), a fenpropathrin-resistant strain (FeR) and an abamectin-resistant strain (AbR), was studied. Laboratory bioassays indicated that the toxicity of celangulin against the SS strain was highest, its LC50 being 1.3883mg/L, compared with pyridaben (53.8446mg/L) and omethoate (116.0337mg/L), and the toxicity of celangulin against FeR (LC50=1.3825mg/L) was almost the same as that of the SS strain, i.e. there was no cross resistance between celangulin and fenpropathrin. But the toxicity of celangulin against AbR (LC50=6.9203mg/L) was inferior to that against SS, and its LC50 value increased 4.98-fold, which showed that some cross resistance existed between celangulin and abamectin. The effect of celangulin E.C against T. cinnabarinus was higher than pyridaben and omethoate in a potted plant experiment. The dose of the celangulin's active ingredient that was needed to ob...

Haijian Wang - One of the best experts on this subject based on the ideXlab platform.

Guang-lu Shi - One of the best experts on this subject based on the ideXlab platform.

  • Research Article Transcriptome Analysis of the Carmine Spider Mite, Tetranychus cinnabarinus (Boisduval, 1867) (Acari: Tetranychidae), and Its Response to
    2015
    Co-Authors: Xiao-qin Wang, Guang-lu Shi, Bo Peng
    Abstract:

    which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Tetranychus cinnabarinus (Acari: Tetranychidae) is a worldwide polyphagous agricultural pest that has the title of resistance champion among arthropods. We reported previously the identification of the acaricidal compoun

  • Acaricidal Activity of Ethyl Palmitate against Tetranychus cinnabarinus
    Advances in Intelligent and Soft Computing, 2012
    Co-Authors: Dandan Duan, You-nian Wang, Yu-bo Liu, Guang-lu Shi
    Abstract:

    Isolation and identification of bioactive compounds from medicinal plant of Inula flower is a potential approach for the development of new pesticides against the mite of Tetranychus cinnabarinus. Dry Inula Flower was used as the starting material to obtain active substances with acaricidal activity by organic solvent extraction and bioactivity assay verification. The main component of the active substances in fraction 33 was further identified by GC/MS and proven to be ethyl palmitate. In order to clarify the roles of ethyl palmitate playing against mites of Tetranychus cinnabarinus, the enzymatic activities were assayed regarding to four essential enzymes in the process of either nerve conduction or anti-oxidation reaction of the mites, including assays for Ca2+-ATPase, superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD). In addition, the substructures of the ethyl palmitate treated adult female mite of T. cinnabarinus were observed by transmission electron microscopy. In the bioassay, ethyl palmitae showed strong activities against the mites with the corrected mortality rates of more than 90% for samples at 2mg/ml concentration. The activity increases of antioxidant enzymes indicate a certain degree of stimulation of the anti-oxidant mechanisms to response for the ethyl palmitate treatment in vivo. The Ca2+-ATPase plays essential role in the maintenance of nerve cell excitability and conduction. Its enzymatic activity was subject to a certain degree of declination, which might cause the death of the mites due to suppression in nerve conduction. The toxicity effect of the mites by ethyl palmitate was further confirmed by TEM observation. The significant destruction of the skin structures and cellular substructures can directly affect the normal function of the cells, and ultimately lead to the death of the mites. Based on the strong acaricidal activity of ethyl palmitate has against the carmine spider mite, it is valuable to be further investigated for a new type of botanical pesticides development.

  • Acaricidal Activity of the Oleic Acid Methyl Ester from Pharbitis purpurea Seeds against Tetranychus cinnabarinus
    Advances in Intelligent and Soft Computing, 2012
    Co-Authors: Lei Zhao, You-nian Wang, Yu-bo Liu, Guang-lu Shi, Bao-sheng Tong
    Abstract:

    Acaricidal activities of the extraction of Pharbitis purpurea seeds against Tetranychus cinnabarinus were determined and fraction 8 was confirmed to be the active components. GC-MS analysis showed that fraction 8 was mainly composed of methyl oleate. Effects of the methyl oleate against Tetranychus cinnabarinus were investigated by slide-dip method and leaf-dip method. Effects of methyl oleate on the organization of Tetranychus cinnabarinus were observed by transmission electron microscope (TEM). The results revealed that the methyl oleate showed killing effects on Tetranychus cinnabarinus and inhibitory effects on the growth of the eggs. After treated with 5 mg/mL methyl oleate for 24 h, the corrected mortalities of Tetranychus cinnabarinus and eggs were 73.68% and 76.16 %, respectively, and the LC50 values were 1.1671 mg/mL and 0.9533 mg/mL, respectively. The inhibitory rates of eggs of Tetranychus cinnabarinus were 78.46%. TEM observation showed that the skin structure, muscle fiber, nuclear membrane, mitochondria, and endoplasmic reticulum of mites were damaged to varying degrees by the methyl oleate. Thus, the methyl oleate from the extraction of Pharbitis purpurea seeds showed a high acaricidal activity, and provided a theoretical basis for the development of botanical acaricides.

  • Insecticidal Activity of Juglans regia Extracts against Tetranychus cinnabarinus and Their Effects on Relative Enzymes Activity in Tetranychus cinnabarinus
    Advances in Intelligent and Soft Computing, 2012
    Co-Authors: You-nian Wang, Yu-bo Liu, Haixiang Wang, Guang-lu Shi
    Abstract:

    The experiment was conducted to determine the insecticidal activity of three different extracts from walnut green husk against Tetranychus cinnabarinus. The results showed that the female insect mortality was 83.44 % at 24 h after exposed in the petroleum ether extract(1 mg·mL-1) from walnut green husk, which was significantly higher than that in the chloroform and methanol extracts (P < 0.05) . After parted and purified by extracting and chromatography, it was found that the acaricidal activity of petroleum ether part from crude extracts was significantly more than the methanol part in the concentration of 1 mg·mL-1, and the female insect mortality reached 84.39 % at 24 h after exposed in the petroleum ether part. There were 19 parts gained from petroleum ether part by chromatography, and the insecticidal activity of 4 parts (6th, 7th, 13th, 17th parts) was higher than other parts, which mortality to female insects was more than 60 %. And the purity of 6th part has been above 90 % by HPLC. After detailed gradient detection, it was found that the extraction rate of 6th part from crude extracts was 6.34% in the concentration of 10mg·mL-1, and the female insect mortality was 98.45 %. On the other hand, the effect of 6th part on relative enzymes activity in Tetranychus cinnabarinus was also researched by colorimetric method. The results showed that the activity of AchE and Na+, K+-ATPase in Tetranychus cinnabarinus were 0.47 and 0.62 times as the control after the exposure in the concentration of 10 mg·mL-1 at 8 hours.

  • Acaricidal Activity of Stigmasterol from Inula Britannica against Tetranychus cinnabarinus
    Advances in Intelligent and Soft Computing, 2012
    Co-Authors: Jun Cheng, Dandan Duan, You-nian Wang, Yu-bo Liu, Guang-lu Shi
    Abstract:

    The activity of fraction 12 identified as stigmasterol by GC/MS from the petroleum ether extract of Inula britannica was evaluated against Tetranychus cinnabarinus in laboratory. The bioactivities and mechanism of stigmasterol against Tetranychus cinnabarinus was further investigated. Superoxide dismutase, peroxidase and catalase in T. cinnabarinus treated with stigmasterol were examined through biochemical methods. The ultrastructure of the treated mites was observed by using transmission electron microscope (TEM). The stigmasterol showed lethal effects on the mite adults, and corrected mortality was over 50% with the concentration of 2 mg/ml. Superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in treated mites were enhanced in activity. The results showed that stigmasterol can destroy the balance between oxidation and anti-oxidation. Glutathione-S-transferase (GSTs) activity was strongly induced after treatment. The results indicated that stigmasterol was perhaps attributing to over excitation and dead of treated mites. TEM observations revealed that the treated mites suffered from the ruptures of cuticle, muscular filament, nuclear membrane, mitochondria and endoplasmic reticulum. Thus, stigmasterol appears a strong acaricidal activity against Tetranychus cinnabarinus, can be exploited as new resources for developing potential botanical acaricides.

Zhao Zhimo - One of the best experts on this subject based on the ideXlab platform.

  • Resistance selection and relative fitness of Tetranychus cinnabarinus(Boisduval)to abamectin
    Journal of Plant Protection, 2004
    Co-Authors: Zhao Zhimo
    Abstract:

    Through resistance selection,the life table of abamectin-resistant and susceptiblestrains of Tetranychus cinnabarinus on cotton and cowpea were established in laboratory to eval-uate the relative fitness of two strains.The results showed that after 42 generations selection,T.cinnabarinus developed 8.7-fold resistance to abamectin-EC.Compared with the relativesusceptible strain,the duration of nymph of the resistant strain shortened 0.55d,and the gen-eration time extended 0.88d when fed on cotton.If fed on cowpea,the duration of egg short-ened by 1.71d,nymph stage elongated 0.41 d,the generation time shortened 2.06d,and thefecundity decreased 84.29 eggs per female.The relative fitness of the abamectin-resistantstrains fed on cotton and cowpea were 0.89 and 0.58,respectively.The results also revealedthat the abamectin-resistant strain of T.cinnabarinus had fitness cost.

  • ESTMATION OF REALIZED HERITABILITY OF RESISTANCE TO FENPROPATHRIN,ABAMECTIN,PYRIDABEN AND THEIR MIXTURES IN ACARICIDE-SELECTED STRAINS OF Tetranychus cinnabarinus (BOIDUVAL)
    Insect Science, 2003
    Co-Authors: Zhao Zhimo, Deng Xin‐ping, Wang Jinjun, Liu Huai
    Abstract:

    Threshold trait analysis was used to estimate realized heritability (h 2) of re sistance to five acaricides (three single acaricide and two mixtures) and resistance risk in Tetranychus cinnabarinus (Boiduval). Tetranychus cinnabarinus collected from the field of Beibei, Chongqing reared more than 60 generations under pesticide free conditions and considered s usceptible. Successively selected for about 30 generations, the strain had a 65.55-, 5.82 - , 1.23-, 5.20- and 1.42-fold increase in resistance to fenpropathrin, abam ectin, pyridaben, pyridab en-abamectin (pyridaben: abamectin=7.4:0.1, m/m) and fenpropathrin-abamectin (fenpropathri n: abamectin=8.9:0.1, m/m), respectively. The realized heritability of resista nce t o fenpropathrin, abamectin, pyridaben, pyridaben-abamectin (pyridaben: abamectin=7.4:0.1, m/m) and fenpropathrin-abamectin (fenpropathrin: abamectin=8.9:0.1, m/m) is 0.2167, 0 .0967, 0.0130, 0.0800 and 0.0172, respectively. Under the selected condition, a 10-fold incr ease in resistance would be expected 15 generations for fenpropathrin, 34 generations for abamectin , 333 generations for pyridaben, 42 generations for pyridaben-abamectin (pyridaben: a bamectin=7.4:0.1, m/m) and 200 generations for fenpropathrin-abametcin (fenpropathrin: abamectin= 8.9:0.1, m/m). The highest resistance risk of the five acaricides in Tetranychus cinnabarinus was fenpropathrin, then abamection, pyridaben-abamectin (pyridaben: abamectin=7.4:0.1, m/m), fen propathrin- abamectin (fenpropathrin: abamectin=8.9:0.1, m/m) and that to py ridaben was the lowest. The mixture of pyridaben and abamectin is not useful in delaying development of resistance in the pest to the two single acaricide while the mixture of fenpropathrin and abamectin could do it.

  • Study on Resistance Selection and Activity of Detoxifieation Enzyme in Tetranychus cinnabarinus (Boiduval)
    Chinese Journal of Pesticide Science, 2003
    Co-Authors: Tan Shi-lu, Zhao Zhimo
    Abstract:

    Fenpropathrin and abamectin were used to select the resistance in Tetranychus cinnabarinus in (laboratory.) After 42 and 40 generations selection with abamectin and fenpropathrin, Tetranychus (cinnabarinus) developed 8.7 and 68.5-fold resistance to the two acaricides respectively. The major resistant mechanism to abamectin was the increasing of the activity of carboxylesterases (CarE), glutathion (S-(transferase)) (GSTs) and mixed function oxidase (MFO), and the activity in resistant strain developed 2.7, (3.4 and )1.4-fold contrasted to susceptible strain respectively. The activity of GSTs in the strain (selected) with fenpropathrin developed 2.8 fold contrast to susceptible strain, which meant the resistance was related with the increasing of the activity of GSTs in resistant strain. The result of the kinetic determination of CarE showed that the structure of CarE in the strain selected with abamectin has been changed.

  • ESTMATION OF REALIZED HERITABILITY OF RESISTANCE TO FENPROPATHRIN, ABAMECTIN, PYRIDABEN AND THEIR MIXTURES IN ACARICIDE‐SELECTED STRAINS OF Tetranychus cinnabarinus (BOIDUVAL)
    Insect Science, 2003
    Co-Authors: He Lin, Zhao Zhimo, Wang Jinjun, Deng Xin‐ping, Liu Huai
    Abstract:

    Threshold trait analysis was used to estimate realized heritability (h 2) of re sistance to five acaricides (three single acaricide and two mixtures) and resistance risk in Tetranychus cinnabarinus (Boiduval). Tetranychus cinnabarinus collected from the field of Beibei, Chongqing reared more than 60 generations under pesticide free conditions and considered s usceptible. Successively selected for about 30 generations, the strain had a 65.55-, 5.82 - , 1.23-, 5.20- and 1.42-fold increase in resistance to fenpropathrin, abam ectin, pyridaben, pyridab en-abamectin (pyridaben: abamectin=7.4:0.1, m/m) and fenpropathrin-abamectin (fenpropathri n: abamectin=8.9:0.1, m/m), respectively. The realized heritability of resista nce t o fenpropathrin, abamectin, pyridaben, pyridaben-abamectin (pyridaben: abamectin=7.4:0.1, m/m) and fenpropathrin-abamectin (fenpropathrin: abamectin=8.9:0.1, m/m) is 0.2167, 0 .0967, 0.0130, 0.0800 and 0.0172, respectively. Under the selected condition, a 10-fold incr ease in resistance would be expected 15 generations for fenpropathrin, 34 generations for abamectin , 333 generations for pyridaben, 42 generations for pyridaben-abamectin (pyridaben: a bamectin=7.4:0.1, m/m) and 200 generations for fenpropathrin-abametcin (fenpropathrin: abamectin= 8.9:0.1, m/m). The highest resistance risk of the five acaricides in Tetranychus cinnabarinus was fenpropathrin, then abamection, pyridaben-abamectin (pyridaben: abamectin=7.4:0.1, m/m), fen propathrin- abamectin (fenpropathrin: abamectin=8.9:0.1, m/m) and that to py ridaben was the lowest. The mixture of pyridaben and abamectin is not useful in delaying development of resistance in the pest to the two single acaricide while the mixture of fenpropathrin and abamectin could do it.

Zhimo Zhao - One of the best experts on this subject based on the ideXlab platform.

  • Relative fitness of Tetranychus cinnabarinus resistant strains at different temperatures
    Ying yong sheng tai xue bao = The journal of applied ecology, 2008
    Co-Authors: Chuan-hua Xue, Zhimo Zhao, Jin-jun Wang
    Abstract:

    Based on resistance selection, the life tables of abameetin-resistant (AbR), fenpropathrin-resistant (FeR), and susceptible (S) strains of Tetranychus cinnabarinus at 15 degrees C, 20 degrees C, 25 C, 28 degrees C, 31 degrees C, 34 degrees C, and 36 degrees C were constructed, and the development duration, fertility, and intrinsic rate of increase (r(m)) of the strains were compared, aimed to understand the relative fitness of T. cinnabarinus resistant strains at different temperatures. The results indicated that compared with S strain, AbR and FeR strains had longer development duration at 15 degrees C but lower fertility at 20 degrees C-28 degrees C, and their relative fitness (represented by r(m)) was smaller at 15 degrees C but similar at 20 degrees C - 31 degrees C. For AbR and FeR strains, the fitness cost was moderate at 15 degrees C but lower at 20 degrees C - 31 degrees C. However, at high temperatures ( > or =34 degrees C), FeR strain possessed remarkable fitness cost while AbR strain showed better fitness.

  • Action mechanism and biological activity of celangulin to Tetranychus cinnabarinus (Acari: Tetranychidae)
    Systematic & Applied Acarology, 2004
    Co-Authors: Wei Ding, Zhimo Zhao, Huiying Tao, Jin-jun Wang
    Abstract:

    Abstract The insecticidal activity of 2% Celangulin E.C against some populations of Tetranychus cinnabarinus, including a susceptible strain (SS), a fenpropathrin-resistant strain (FeR) and an abamectin-resistant strain (AbR), was studied. Laboratory bioassays indicated that the toxicity of celangulin against the SS strain was highest, its LC50 being 1.3883mg/L, compared with pyridaben (53.8446mg/L) and omethoate (116.0337mg/L), and the toxicity of celangulin against FeR (LC50=1.3825mg/L) was almost the same as that of the SS strain, i.e. there was no cross resistance between celangulin and fenpropathrin. But the toxicity of celangulin against AbR (LC50=6.9203mg/L) was inferior to that against SS, and its LC50 value increased 4.98-fold, which showed that some cross resistance existed between celangulin and abamectin. The effect of celangulin E.C against T. cinnabarinus was higher than pyridaben and omethoate in a potted plant experiment. The dose of the celangulin's active ingredient that was needed to ob...

  • Resistance Selection of Tetranychus cinnabarinus to Three Acaricides and Its Management Strategy
    Agricultural Sciences in China, 2003
    Co-Authors: Zhimo Zhao, Xin-ping Deng, Jin-jun Wang, Huai Liu, Ying-hong Liu
    Abstract:

    The effects of retarding resistance evolution of mixing and sequencing acaricides were studied based on the resistance selection of three acaricides to Tetranychus cinnabarinus in the laboratory. Tetranychus cinnabarinus was continuously selected with fenpropathrin, abamectin and pyridaban for 28, 30 and 31 generations, respectively, and resistance factor increased to 65.6, 7.2 and 1.2 time, accordingly. Results of cross-resistance determination suggested that there were no cross-resistance existed among the three acaricides. Analysis of resistance selection and estimation of realized heritability (h^2) of resistance to three single and two mixed acaricides showed that a mixture of fenpropathrin+abamectin would slow resistance development compared with a sequence of fenpropathrin followed by abamectin while the mixture of pyridaben+abamectin and the sequence of pyridaben followed by abamectin would effectively retard resistance evolution.

  • Pesticide resistance of Tetranychus cinnabarinus (Acari: Tetranychidae) in China: a review
    Systematic & Applied Acarology, 1998
    Co-Authors: Fengying Guo, Zhi-qiang Zhang, Zhimo Zhao
    Abstract:

    This paper reviews the development of pesticide resistance in Tetranychus cinnabarinus (Boisduval) and studies on the genetics and management of pesticide resistance in China. This mite has developed resistance to at least 25 pesticides in China. It first developed resistance to parathion and demeton. As early as 1962, the level of resistance to demeton in T. cinnabarinus reached 62 times, which resulted in control failure of this mite on cotton in most cotton growing areas in China. The genetics of omethoate-resistance and monocrotophos-resistance in T. cinnabarinus is each controlled by an incompletely dominant gene and the development of resistance is therefore rapid. The developmental time and other life table parameters of T. cinnabarinus are altered when the mite becomes pesticide-resistant: life cycle becomes shorter and reproductive rate becomes greater. This is another reason for the rapid development of pesticide resistance in T. cinnabarinus. Pesticide resistance may be managed by rotational use of pesticides, biological control and other methods.