The Experts below are selected from a list of 123 Experts worldwide ranked by ideXlab platform
Felgueiras, Helena Prado - One of the best experts on this subject based on the ideXlab platform.
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Spun bioTextiles in tissue engineering and biomolecules delivery systems
'MDPI AG', 2020Co-Authors: Miranda, Catarina S., Ribeiro, Ana R. M., Homem, Natália Cândido, Felgueiras, Helena PradoAbstract:Nowadays, tissue engineering is described as an interdisciplinary field that combines engineering principles and life Sciences to generate implantable devices to repair, restore and/or improve functions of injured tissues. Such devices are designed to induce the interaction and integration of tissue and cells within the implantable matrices and are manufactured to meet the appropriate physical, mechanical and physiological local demands. Biodegradable constructs based on polymeric fibers are desirable for tissue engineering due to their large surface area, interconnectivity, open pore structure, and controlled mechanical strength. Additionally, biodegradable constructs are also very sought-out for biomolecule delivery systems with a target-directed action. In the present review, we explore the properties of some of the most common biodegradable polymers used in tissue engineering applications and biomolecule delivery systems and highlight their most important uses.Authors acknowledge the Portuguese Foundation for Science and Technology (FCT), FEDER funds by means of Portugal 2020 Competitive Factors Operational Program (POCI) and the Portuguese Government (OE) for funding the project PEPTEX with reference PTDC/CTM-TEX/28074/2017 (POCI-01-0145- FEDER-028074). Authors also acknowledge project UID/CTM/00264/2020 of Centre for Textile Science and Technology (2C2T), funded by national funds through FCT/MCTES
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New method to produce poly(vinyl alcohol)/cellulose acetate films with improved antibacterial action
Elsevier, 2020Co-Authors: Felgueiras, Helena Prado, Teixeira, Marta Albertina, Tavares, Tânia Daniela Eugénio, M. Pessoa T. De ,amorimAbstract:New alternatives to the conventional wound dressings are being engineered. Here, we propose the processing of two biodegradable polymers, poly(vinyl alcohol) (PVA) and cellulose acetate (CA), in the form of films via a new method that combines principles of solvent casting and phase-inversion. Highly flexible and mechanically resistant films were obtained. PVA/CA films were then treated with the antibiotic vancomycin via two methods, blending and physisorption (via dopamine). Immobilization of vancomycin was proven efficient in promoting the films' antibacterial action against Staphylococcus epidermidis bacteria. Data demonstrates the potentialities of PVA/CA films for prospective wound healing applications.Authors acknowledge the Portuguese Foundation for Science and Technology (FCT), FEDER funds by means of Portugal 2020 Competitive Factors Operational Program (POCI) and the Portuguese Government (OE) for funding the project PEPTEX with reference POCI-01-0145-FEDER-028074. Authors also acknowledge project UID/CTM/00264/2019 of Centre for Textile Science and Technology (2C2T), funded by national funds through FCT/MCTE
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Dog wool microparticles/polyurethane composite for thermal insulation
'MDPI AG', 2020Co-Authors: Francisco Claudivan Da ,silva, Felgueiras, Helena Prado, Ladchumananandasivam Rasiah, Mendes, José Ubiragi L., Silva, Késia Karina De O. Souto, Zille AndreaAbstract:A polyurethane (PU)-based eco-composite foam was prepared using dog wool fibers as a filler. Fibers were acquired from pet shops and alkaline treated prior to use. The influence of their incorporation on the PU foams’ morphological, thermal, and mechanical properties was investigated. The random and disorganized presence of the microfibers along the foam influence their mechanical performance. Tensile and compression strengths were improved with the increased amount of dog wool microparticles on the eco-composites. The same occurred with the foams’ hydration capacity. The thermal capacity was also slightly enhanced with the incorporation of the fillers. The fillers also increased the thermal stability of the foams, reducing their dilatation with heating. The best structural stability was obtained using up to 120 °C with a maximum of 15% of filler. In the end, the dog wool waste was rationally valorized as a filler in PU foams, demonstrating its potential for insulation applications, with a low cost and minimal environmental impact.Authors acknowledge the Fundação de Apoio a Pesquisa do Estado do Rio Grande do Norte (FAPERN) for financing this work. They thank the pet shops from Natal city for donating the dog wool fibers used in the experiments. H.P. Felgueiras and A. Zille also acknowledge project UID/CTM/00264/2019 of Centre for Textile Science and Technology (2C2T), funded by national funds through FCT/MCTES
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Antimicrobial action and clotting time of thin, hydrated poly(Vinyl Alcohol)/cellulose acetate films functionalized with LL37 for prospective wound-healing applications
'Wiley', 2020Co-Authors: Felgueiras, Helena Prado, Homem, Natália Cândido, Teixeira, Marta Albertina, Tavares, Tânia Daniela Eugénio, Zille Andrea, M. Pessoa T. De ,amorimAbstract:Poly(vinyl alcohol)/cellulose acetate (PVA/CA) films were prepared via a new method that combines principles from solvent casting and phase inversion. To guarantee some degree of flexibility, films were produced with a higher percentage of PVA compared to CA, from 90/10 to 50/50. The antimicrobial peptide (AMP) LL37 was then anchored using dopamine as a binding agent. Films were characterized in terms of functional groups, thermal stability, tensile strength, porosity, swelling and degradation (stability in physiological media at different pHs). The antimicrobial performance of LL37 surface-modified films was tested against Staphylococcus aureus, Staphylococcus epidermidis and Escherichia coli in dynamic environment and in the presence and absence of an albumin interface. LL37 treated films demonstrated great antibacterial efficacy against the three kinds of bacteria, ≈ 75% inhibition for S. aureus, ≈ 85% for S. epidermidis and ≈ 60% for E. coli, regardless of PVA/CA ratio. Presence of albumin reduced bacteria inhibition in all tested groups, most likely due to the binding of the protein molecules to the antimicrobial agents, reducing the free fraction available for bacterial killing. Films treated with LL37 accelerated clotting time (≈ 10 min) above vancomycin and bare surfaces, demonstrating great capacity to activate the intrinsic coagulation cascade.Portuguese Foundation for Science and Technology (FCT), FEDER funds by means of Portugal 2020 Competitive Factors Operational Program (POCI) and the Portuguese Government (OE) for funding the project PEPTEX with reference POCI-01-0145-FEDER-028074. Authors also acknowledge project UID/CTM/00264/2019 of Centre for Textile Science and Technology (2C2T), funded by national funds through FCT/MCTE
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Electrospun nanocomposites containing cellulose and Its derivatives modified with specialized biomolecules for an enhanced wound healing
'MDPI AG', 2020Co-Authors: Teixeira, Marta A., M. Pessoa T. De ,amorim, Paiva M. C., Felgueiras, Helena PradoAbstract:Wound healing requires careful, directed, and effective therapies to prevent infections and accelerate tissue regeneration. In light of these demands, active biomolecules with antibacterial properties and/or healing capacities have been functionalized onto nanostructured polymeric dressings and their synergistic effect examined. In this work, various antibiotics, nanoparticles, and natural extract-derived products that were used in association with electrospun nanocomposites containing cellulose, cellulose acetate and different types of nanocellulose (cellulose nanocrystals, cellulose nanofibrils, and bacterial cellulose) have been reviewed. Renewable, natural-origin compounds are gaining more relevance each day as potential alternatives to synthetic materials, since the former undesirable footprints in biomedicine, the environment, and the ecosystems are reaching concerning levels. Therefore, cellulose and its derivatives have been the object of numerous biomedical studies, in which their biocompatibility, biodegradability, and, most importantly, sustainability and abundance, have been determinant. A complete overview of the recently produced cellulose-containing nanofibrous meshes for wound healing applications was provided. Moreover, the current challenges that are faced by cellulose acetate- and nanocellulose-containing wound dressing formulations, processed by electrospinning, were also enumerated.This research received funding from the Portuguese Foundation for Science and Technology (FCT) under the scope of the projects PTDC/CTM-TEX/28074/2017 (POCI-01-0145-FEDER-028074) and UID/CTM/00264/2020.Authors acknowledge the Portuguese Foundation for Science and Technology (FCT), FEDER funds by means of Portugal 2020 Competitive Factors Operational Program (POCI) and the Portuguese Government (OE) for funding the project PEPTEX with reference PTDC/CTM-TEX/28074/2017 (POCI-01-0145-FEDER-028074). Authors also acknowledge project UID/CTM/00264/2020 of Centre for Textile Science and Technology (2C2T), funded by national funds through FCT/MCTES. M.A.T. also acknowledges FCT for the PhD grant SFRH/BD/148930/2019
M. Pessoa T. De ,amorim - One of the best experts on this subject based on the ideXlab platform.
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New method to produce poly(vinyl alcohol)/cellulose acetate films with improved antibacterial action
Elsevier, 2020Co-Authors: Felgueiras, Helena Prado, Teixeira, Marta Albertina, Tavares, Tânia Daniela Eugénio, M. Pessoa T. De ,amorimAbstract:New alternatives to the conventional wound dressings are being engineered. Here, we propose the processing of two biodegradable polymers, poly(vinyl alcohol) (PVA) and cellulose acetate (CA), in the form of films via a new method that combines principles of solvent casting and phase-inversion. Highly flexible and mechanically resistant films were obtained. PVA/CA films were then treated with the antibiotic vancomycin via two methods, blending and physisorption (via dopamine). Immobilization of vancomycin was proven efficient in promoting the films' antibacterial action against Staphylococcus epidermidis bacteria. Data demonstrates the potentialities of PVA/CA films for prospective wound healing applications.Authors acknowledge the Portuguese Foundation for Science and Technology (FCT), FEDER funds by means of Portugal 2020 Competitive Factors Operational Program (POCI) and the Portuguese Government (OE) for funding the project PEPTEX with reference POCI-01-0145-FEDER-028074. Authors also acknowledge project UID/CTM/00264/2019 of Centre for Textile Science and Technology (2C2T), funded by national funds through FCT/MCTE
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Antimicrobial action and clotting time of thin, hydrated poly(Vinyl Alcohol)/cellulose acetate films functionalized with LL37 for prospective wound-healing applications
'Wiley', 2020Co-Authors: Felgueiras, Helena Prado, Homem, Natália Cândido, Teixeira, Marta Albertina, Tavares, Tânia Daniela Eugénio, Zille Andrea, M. Pessoa T. De ,amorimAbstract:Poly(vinyl alcohol)/cellulose acetate (PVA/CA) films were prepared via a new method that combines principles from solvent casting and phase inversion. To guarantee some degree of flexibility, films were produced with a higher percentage of PVA compared to CA, from 90/10 to 50/50. The antimicrobial peptide (AMP) LL37 was then anchored using dopamine as a binding agent. Films were characterized in terms of functional groups, thermal stability, tensile strength, porosity, swelling and degradation (stability in physiological media at different pHs). The antimicrobial performance of LL37 surface-modified films was tested against Staphylococcus aureus, Staphylococcus epidermidis and Escherichia coli in dynamic environment and in the presence and absence of an albumin interface. LL37 treated films demonstrated great antibacterial efficacy against the three kinds of bacteria, ≈ 75% inhibition for S. aureus, ≈ 85% for S. epidermidis and ≈ 60% for E. coli, regardless of PVA/CA ratio. Presence of albumin reduced bacteria inhibition in all tested groups, most likely due to the binding of the protein molecules to the antimicrobial agents, reducing the free fraction available for bacterial killing. Films treated with LL37 accelerated clotting time (≈ 10 min) above vancomycin and bare surfaces, demonstrating great capacity to activate the intrinsic coagulation cascade.Portuguese Foundation for Science and Technology (FCT), FEDER funds by means of Portugal 2020 Competitive Factors Operational Program (POCI) and the Portuguese Government (OE) for funding the project PEPTEX with reference POCI-01-0145-FEDER-028074. Authors also acknowledge project UID/CTM/00264/2019 of Centre for Textile Science and Technology (2C2T), funded by national funds through FCT/MCTE
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Electrospun nanocomposites containing cellulose and Its derivatives modified with specialized biomolecules for an enhanced wound healing
'MDPI AG', 2020Co-Authors: Teixeira, Marta A., M. Pessoa T. De ,amorim, Paiva M. C., Felgueiras, Helena PradoAbstract:Wound healing requires careful, directed, and effective therapies to prevent infections and accelerate tissue regeneration. In light of these demands, active biomolecules with antibacterial properties and/or healing capacities have been functionalized onto nanostructured polymeric dressings and their synergistic effect examined. In this work, various antibiotics, nanoparticles, and natural extract-derived products that were used in association with electrospun nanocomposites containing cellulose, cellulose acetate and different types of nanocellulose (cellulose nanocrystals, cellulose nanofibrils, and bacterial cellulose) have been reviewed. Renewable, natural-origin compounds are gaining more relevance each day as potential alternatives to synthetic materials, since the former undesirable footprints in biomedicine, the environment, and the ecosystems are reaching concerning levels. Therefore, cellulose and its derivatives have been the object of numerous biomedical studies, in which their biocompatibility, biodegradability, and, most importantly, sustainability and abundance, have been determinant. A complete overview of the recently produced cellulose-containing nanofibrous meshes for wound healing applications was provided. Moreover, the current challenges that are faced by cellulose acetate- and nanocellulose-containing wound dressing formulations, processed by electrospinning, were also enumerated.This research received funding from the Portuguese Foundation for Science and Technology (FCT) under the scope of the projects PTDC/CTM-TEX/28074/2017 (POCI-01-0145-FEDER-028074) and UID/CTM/00264/2020.Authors acknowledge the Portuguese Foundation for Science and Technology (FCT), FEDER funds by means of Portugal 2020 Competitive Factors Operational Program (POCI) and the Portuguese Government (OE) for funding the project PEPTEX with reference PTDC/CTM-TEX/28074/2017 (POCI-01-0145-FEDER-028074). Authors also acknowledge project UID/CTM/00264/2020 of Centre for Textile Science and Technology (2C2T), funded by national funds through FCT/MCTES. M.A.T. also acknowledges FCT for the PhD grant SFRH/BD/148930/2019
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Physical, thermal, and antibacterial effects of active essential oils with potential for biomedical applications loaded onto cellulose acetate/polycaprolactone wet-spun microfibers
'MDPI AG', 2020Co-Authors: Felgueiras, Helena Prado, Ribeiro, Ana R. M., Homem, Natália Cândido, Teixeira, Marta A., Antunes, Joana C., M. Pessoa T. De ,amorimAbstract:New approaches to deal with the growing concern associated with antibiotic-resistant bacteria are emerging daily. Essential oils (EOs) are natural antimicrobial substances with great potential to mitigate this situation. However, their volatile nature, in their liquid-free form, has restricted their generalized application in biomedicine. Here, we propose the use of cellulose acetate (CA)/polycaprolactone (PCL) wet-spun fibers as potential delivery platforms of selected EOs to fight infections caused by Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). Twenty EOs were selected and screened for their minimal inhibitory concentration (MIC), using the antibiotic ampicillin as positive control. The cinnamon leaf oil (CLO), cajeput oil (CJO), and the clove oil (CO) were the most effective EOs, against the Gram-positive (MIC < 22.38 mg/mL) and the Gram-negative (MIC < 11.19 mg/mL) bacteria. Uniform microfibers were successfully wet-spun from CA/PCL with an averaged diameter of 53.9 ± 4.5 µm, and then modified by immersion with CLO, CJO and CO at 2 × MIC value. EOs incorporation was confirmed by UV-visible spectroscopy, Fourier-transformed infrared spectroscopy, and thermal gravimetric analysis. However, while microfibers contained ampicillin at MIC (control) after the 72 h modification, the CLO, CO and CJO-loaded fibers registered ≈ 14%, 66%, and 76% of their MIC value, respectively. Data showed that even at small amounts the EO-modified microfibers were effective against the tested bacteria, both by killing bacteria more quickly or by disrupting more easily their cytoplasmic membrane than ampicillin. Considering the amount immobilized, CLO-modified fibers were deemed the most effective from the EOs group. These results indicate that CA/PCL microfibers loaded with EOs can be easily produced with increased antibacterial action, envisioning their use as scaffolding materials for the treatment of infections.Authors acknowledge the Portuguese Foundation for Science and Technology (FCT), FEDER funds by means of Portugal 2020 Competitive Factors Operational Program (POCI) and the Portuguese Government(OE) for funding the project PEPTEX with reference PTDC/CTM-TEX/28074/2017 (POCI-01-0145-FEDER-028074).Authors also acknowledge project UID/CTM/00264/2020 of Centre for Textile Science and Technology (2C2T),funded by national funds through FCT/MCTES. SEM studies were performed at the Materials CharacterizationServices of the University of Minho (SEMAT/UM)
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PVA/CA based electrospun nanofibers: influence of processing parameters in the fiber diameter
'IOP Publishing', 2019Co-Authors: Teixeira, Marta Albertina, M. Pessoa T. De ,amorim, Felgueiras, Helena PradoAbstract:Recently, the electrospinning technique has been explored as a natural and synthetic polymer processing tool due to its versatility and potential to generate complex structures at a nanoscale. In this work, non-woven nanofibrous mats were electrospun, with a structure resembling the extracellular matrix, for prospective biomedical uses. Poly (vinyl alcohol) (PVA) and cellulose acetate (CA) based electrospun nanofibrous meshes were prepared at different ratios 100/0, 90/10, 80/20 and 70/30 and characterized in terms of fiber diameter. The process was kept as green as possible by resorting to a combination of acetic acid and distilled water as solvents. Optimal conditions for PVA/CA processing were established at 29 kV, feeding rate of 0.8 mL/h and distance between needle and collector of 17 cm. These allowed for the most uniform fibers with the smallest diameters to be produced.Authors acknowledge the Portuguese Foundation for Science and Technology (FCT), FEDER funds by means of Portugal 2020 Competitive Factors Operational Program (POCI) and the Portuguese Government (OE) for funding the project PEPTEX with reference POCI-01-0145-FEDER-028074. Authors also acknowledge project UID/CTM/00264/2019 of Centre for Textile Science and Technology (2C2T), funded by national funds through FCT/MCTES
Teixeira, Marta Albertina - One of the best experts on this subject based on the ideXlab platform.
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New method to produce poly(vinyl alcohol)/cellulose acetate films with improved antibacterial action
Elsevier, 2020Co-Authors: Felgueiras, Helena Prado, Teixeira, Marta Albertina, Tavares, Tânia Daniela Eugénio, M. Pessoa T. De ,amorimAbstract:New alternatives to the conventional wound dressings are being engineered. Here, we propose the processing of two biodegradable polymers, poly(vinyl alcohol) (PVA) and cellulose acetate (CA), in the form of films via a new method that combines principles of solvent casting and phase-inversion. Highly flexible and mechanically resistant films were obtained. PVA/CA films were then treated with the antibiotic vancomycin via two methods, blending and physisorption (via dopamine). Immobilization of vancomycin was proven efficient in promoting the films' antibacterial action against Staphylococcus epidermidis bacteria. Data demonstrates the potentialities of PVA/CA films for prospective wound healing applications.Authors acknowledge the Portuguese Foundation for Science and Technology (FCT), FEDER funds by means of Portugal 2020 Competitive Factors Operational Program (POCI) and the Portuguese Government (OE) for funding the project PEPTEX with reference POCI-01-0145-FEDER-028074. Authors also acknowledge project UID/CTM/00264/2019 of Centre for Textile Science and Technology (2C2T), funded by national funds through FCT/MCTE
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Antimicrobial action and clotting time of thin, hydrated poly(Vinyl Alcohol)/cellulose acetate films functionalized with LL37 for prospective wound-healing applications
'Wiley', 2020Co-Authors: Felgueiras, Helena Prado, Homem, Natália Cândido, Teixeira, Marta Albertina, Tavares, Tânia Daniela Eugénio, Zille Andrea, M. Pessoa T. De ,amorimAbstract:Poly(vinyl alcohol)/cellulose acetate (PVA/CA) films were prepared via a new method that combines principles from solvent casting and phase inversion. To guarantee some degree of flexibility, films were produced with a higher percentage of PVA compared to CA, from 90/10 to 50/50. The antimicrobial peptide (AMP) LL37 was then anchored using dopamine as a binding agent. Films were characterized in terms of functional groups, thermal stability, tensile strength, porosity, swelling and degradation (stability in physiological media at different pHs). The antimicrobial performance of LL37 surface-modified films was tested against Staphylococcus aureus, Staphylococcus epidermidis and Escherichia coli in dynamic environment and in the presence and absence of an albumin interface. LL37 treated films demonstrated great antibacterial efficacy against the three kinds of bacteria, ≈ 75% inhibition for S. aureus, ≈ 85% for S. epidermidis and ≈ 60% for E. coli, regardless of PVA/CA ratio. Presence of albumin reduced bacteria inhibition in all tested groups, most likely due to the binding of the protein molecules to the antimicrobial agents, reducing the free fraction available for bacterial killing. Films treated with LL37 accelerated clotting time (≈ 10 min) above vancomycin and bare surfaces, demonstrating great capacity to activate the intrinsic coagulation cascade.Portuguese Foundation for Science and Technology (FCT), FEDER funds by means of Portugal 2020 Competitive Factors Operational Program (POCI) and the Portuguese Government (OE) for funding the project PEPTEX with reference POCI-01-0145-FEDER-028074. Authors also acknowledge project UID/CTM/00264/2019 of Centre for Textile Science and Technology (2C2T), funded by national funds through FCT/MCTE
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PVA/CA based electrospun nanofibers: influence of processing parameters in the fiber diameter
'IOP Publishing', 2019Co-Authors: Teixeira, Marta Albertina, M. Pessoa T. De ,amorim, Felgueiras, Helena PradoAbstract:Recently, the electrospinning technique has been explored as a natural and synthetic polymer processing tool due to its versatility and potential to generate complex structures at a nanoscale. In this work, non-woven nanofibrous mats were electrospun, with a structure resembling the extracellular matrix, for prospective biomedical uses. Poly (vinyl alcohol) (PVA) and cellulose acetate (CA) based electrospun nanofibrous meshes were prepared at different ratios 100/0, 90/10, 80/20 and 70/30 and characterized in terms of fiber diameter. The process was kept as green as possible by resorting to a combination of acetic acid and distilled water as solvents. Optimal conditions for PVA/CA processing were established at 29 kV, feeding rate of 0.8 mL/h and distance between needle and collector of 17 cm. These allowed for the most uniform fibers with the smallest diameters to be produced.Authors acknowledge the Portuguese Foundation for Science and Technology (FCT), FEDER funds by means of Portugal 2020 Competitive Factors Operational Program (POCI) and the Portuguese Government (OE) for funding the project PEPTEX with reference POCI-01-0145-FEDER-028074. Authors also acknowledge project UID/CTM/00264/2019 of Centre for Textile Science and Technology (2C2T), funded by national funds through FCT/MCTES
Tavares, Tânia Daniela Eugénio - One of the best experts on this subject based on the ideXlab platform.
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New method to produce poly(vinyl alcohol)/cellulose acetate films with improved antibacterial action
Elsevier, 2020Co-Authors: Felgueiras, Helena Prado, Teixeira, Marta Albertina, Tavares, Tânia Daniela Eugénio, M. Pessoa T. De ,amorimAbstract:New alternatives to the conventional wound dressings are being engineered. Here, we propose the processing of two biodegradable polymers, poly(vinyl alcohol) (PVA) and cellulose acetate (CA), in the form of films via a new method that combines principles of solvent casting and phase-inversion. Highly flexible and mechanically resistant films were obtained. PVA/CA films were then treated with the antibiotic vancomycin via two methods, blending and physisorption (via dopamine). Immobilization of vancomycin was proven efficient in promoting the films' antibacterial action against Staphylococcus epidermidis bacteria. Data demonstrates the potentialities of PVA/CA films for prospective wound healing applications.Authors acknowledge the Portuguese Foundation for Science and Technology (FCT), FEDER funds by means of Portugal 2020 Competitive Factors Operational Program (POCI) and the Portuguese Government (OE) for funding the project PEPTEX with reference POCI-01-0145-FEDER-028074. Authors also acknowledge project UID/CTM/00264/2019 of Centre for Textile Science and Technology (2C2T), funded by national funds through FCT/MCTE
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Antimicrobial action and clotting time of thin, hydrated poly(Vinyl Alcohol)/cellulose acetate films functionalized with LL37 for prospective wound-healing applications
'Wiley', 2020Co-Authors: Felgueiras, Helena Prado, Homem, Natália Cândido, Teixeira, Marta Albertina, Tavares, Tânia Daniela Eugénio, Zille Andrea, M. Pessoa T. De ,amorimAbstract:Poly(vinyl alcohol)/cellulose acetate (PVA/CA) films were prepared via a new method that combines principles from solvent casting and phase inversion. To guarantee some degree of flexibility, films were produced with a higher percentage of PVA compared to CA, from 90/10 to 50/50. The antimicrobial peptide (AMP) LL37 was then anchored using dopamine as a binding agent. Films were characterized in terms of functional groups, thermal stability, tensile strength, porosity, swelling and degradation (stability in physiological media at different pHs). The antimicrobial performance of LL37 surface-modified films was tested against Staphylococcus aureus, Staphylococcus epidermidis and Escherichia coli in dynamic environment and in the presence and absence of an albumin interface. LL37 treated films demonstrated great antibacterial efficacy against the three kinds of bacteria, ≈ 75% inhibition for S. aureus, ≈ 85% for S. epidermidis and ≈ 60% for E. coli, regardless of PVA/CA ratio. Presence of albumin reduced bacteria inhibition in all tested groups, most likely due to the binding of the protein molecules to the antimicrobial agents, reducing the free fraction available for bacterial killing. Films treated with LL37 accelerated clotting time (≈ 10 min) above vancomycin and bare surfaces, demonstrating great capacity to activate the intrinsic coagulation cascade.Portuguese Foundation for Science and Technology (FCT), FEDER funds by means of Portugal 2020 Competitive Factors Operational Program (POCI) and the Portuguese Government (OE) for funding the project PEPTEX with reference POCI-01-0145-FEDER-028074. Authors also acknowledge project UID/CTM/00264/2019 of Centre for Textile Science and Technology (2C2T), funded by national funds through FCT/MCTE
T. Matsuo - One of the best experts on this subject based on the ideXlab platform.
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Material design and Textile Science for specialty Textiles technologies
International Journal of Clothing Science and Technology, 2004Co-Authors: T. MatsuoAbstract:The relation of material design and Textile Science to the technologies of Textile specialty products are discussed. Concept of material design is explained. The method of differential total material design is presented with an application example. The relation of material design to specialty Textiles technology is analyzed. The relation of Textile Science to material design in terms of specialty Textiles technology is also discussed. Some examples of scientific knowledge in terms of data base for material design are presented.
