The Experts below are selected from a list of 249 Experts worldwide ranked by ideXlab platform

Alissara Reungsang - One of the best experts on this subject based on the ideXlab platform.

  • two stage thermophilic bio hydrogen and methane production from oil palm trunk hydrolysate using Thermoanaerobacterium thermosaccharolyticum kku19
    International Journal of Hydrogen Energy, 2017
    Co-Authors: Saruda Sitthikitpanya, Alissara Reungsang, P Prasertsan, Samir Kumar Khanal
    Abstract:

    Abstract The two-stage process for thermophilic bio-hydrogen production followed by methane production was examined on a hydrolysate obtained from oil palm trunk (OPT). The optimum conditions for lime pretreatment and enzymatic hydrolysis of OPT was a lime loading of 0.2 g Ca(OH)2/g-OPT, pretreatment time of 60 min, temperature of 121 °C and enzyme loading of 35 filter paper units/g-OPT. A maximum total reducing sugar yield of 473 mg/g-OPT was obtained, which was 2.7-fold higher than that of untreated OPT. The OPT hydrolysate was used as a substrate for bio-hydrogen production by Thermoanaerobacterium thermosaccharolyticum KKU19 in the first stage. The maximum hydrogen production potential of 2179 mL H2/Lsubstrate was obtained under the optimum conditions of 16.5 g/L initial substrate concentration, initial pH of 6.7 and 54.5 °C, respectively. Acidic effluent was used to produce methane in the second stage in which the methane yield of 272.4 mL/g-chemical oxygen demand (COD) was achieved. The two-stage hydrogen and methane production resulted in energy yield of 10.6 kJ/g-CODadded with 83% COD removal.

  • co digestion of oil palm trunk hydrolysate with slaughterhouse wastewater for thermophilic bio hydrogen production by Thermoanaerobacterium thermosaccharolyticm kku19
    International Journal of Hydrogen Energy, 2014
    Co-Authors: Sontaya Khamtib, Alissara Reungsang
    Abstract:

    Abstract The key factors influencing a co-digestion of the oil palm trunk (OPT) hydrolysate with a slaughterhouse wastewater (SHW) to produce hydrogen by Thermoanaerobacterium thermosaccharolyticum KKU19 were investigated. The OPT hydrolysate was obtained by the hydrolysis of OPT by microwave-H 2 SO 4 method using 1.56% (w/v) H 2 SO 4 and 7.50 min reaction time at 450 W. The Plackett–Burman method was used to screen the key factors that influenced the hydrogen production potential ( P s ). Results indicated that initial cell concentration, tCOD/TN (total COD/total nitrogen) ratio and CuSO 4 concentration influenced the P s . These factors were further optimized using response surface methodology (RSM) with central composite design (CCD). A maximum P s of 2604 ± 86 mL H 2 /L substrate was achieved at an initial cell concentration of 224 mg dry cell/L, tCOD/TN ratio of 49.87 and CuSO 4 concentration of 13.33 mg/L. The main soluble metabolite products were butyric and acetic acids. The P s obtained when the hydrolysate was supplemented with SHW (2604mL ± 86 mL H 2 /L substrate) was comparable to the P s obtained when it was supplemented with yeast extract at the same tCOD/TN (2802 ± 87 mL H 2 /L substrate). This result suggests that SHW can be used to replace the costly nitrogen source.

  • thermophilic biohydrogen production from the enzymatic hydrolysate of cellulose fraction of sweet sorghum bagasse by Thermoanaerobacterium thermosaccharolyticum kku19 optimization of media composition
    International Journal of Hydrogen Energy, 2013
    Co-Authors: Onwita Boonsayompoo, Alissara Reungsang
    Abstract:

    Abstract The composition of media for thermophilic biohydrogen production from the enzymatic hydrolysate of cellulose fraction of sweet sorghum bagasse by Thermoanaerobacterium thermosaccharolyticum KKU19 were optimized in order to maximize the hydrogen production potential (Ps). Results from Plackett-Burman design indicated that FeSO4, CaCl2, NaHCO3, and MgCl2 had a significantly effect (P ≤ 0.05) on Ps. The optimum media composition obtained from the response surface methodology (RSM) with central composite design (CCD), using the hydrolysate at a total sugar concentration of 8.98 g/L, were (all in mg/L): FeSO4, 1454.65; MgCl2, 511.36; CaCl2, 278.62; and NaHCO3, 2186.41 in which the Ps of 2397 mL H2/L were obtained. Verification experiment using the optimum media composition in a continuous stirred tank reactor indicated a highly reproducible result in which the Ps of 2608 mL H2/L was achieved at a hydraulic retention time of 32 h. The results demonstrated that the media composition obtained from the batch experiment using RSM with CCD can be practically applied to continuously produce hydrogen from the hydrolysate with the least error.

  • biohydrogen production by Thermoanaerobacterium thermosaccharolyticum kku ed1 culture conditions optimization using xylan as the substrate
    International Journal of Hydrogen Energy, 2013
    Co-Authors: Arunsri Fangkum Saripan, Alissara Reungsang
    Abstract:

    Abstract Thermophilic hydrogen production from xylan by Thermoanaerobacterium thermosaccharolyticum KKU-ED1 isolated from elephant dung was investigated using batch fermentation. The optimum conditions for hydrogen production from xylan by the strain KKU-ED1 were an initial pH of 7.0, temperature of 55 °C and xylan concentration of 15 g/L. Under the optimum conditions, the hydrogen yield (HY), hydrogen production rate (HPR) and xylanase activity were 120.05 ± 15.07 mL H 2 /g xylan, 11.53 ± 0.19 mL H 2 /L h and 0.41 units/mL, respectively. The optimum conditions were then used to produce hydrogen from 62.5 g/L sugarcane bagasse (SCB) (equivalent to 15 g/L xylan) in which the HY and HPR of 1.39 ± 0.10 mL H 2 /g SCB (5.77 ± 0.41 mL H 2 /g xylan) and 0.66 ± 0.04 mL H 2 /L h, respectively, were achieved. In comparison to the other strains, the HY of the strain KKU-ED1 (120.05 ± 15.07 mL H 2 /g xylan) was close to that of Clostridium sp. strain X53 (125.40 mL H 2 /g xylan) and Clostridium butyricum CGS5 (90.70 mL H 2 /g xylan hydrolysate).

  • biohydrogen production by Thermoanaerobacterium thermosaccharolyticum kku ed1 culture conditions optimization using mixed xylose arabinose as substrate
    Electronic Journal of Biotechnology, 2013
    Co-Authors: Arunsri Fangkum Saripan, Alissara Reungsang
    Abstract:

    Background: Biological hydrogen production by microorganisms can be divided into two main categories i.e. photosynthetic organisms that produce hydrogen using light as energy source and anaerobic bacteria that produce hydrogen via dark fermentation. Dark fermentative hydrogen production by anaerobic bacteria has the advantages of a higher HPR without illumination and of the capability to convert various kinds of substrate. Results: Thermophilic hydrogen producer was isolated from elephant dung and identified as Thermoanaerobacterium thermosaccharolyticum KKU-ED1 by 16S rRNA gene analysis, which was further used to produce hydrogen from mixed pentose sugar i.e., xylose/arabinose. The optimum conditions for hydrogen production from mixed xylose/arabinose by KKU-ED1 were a 1:1 xylose/arabinose mixture at the total concentration of 5 g/L, initial pH of 6.5 and temperature of 55oC. Under the optimum conditions, hydrogen from sugar derived from acid-hydrolyzed sugarcane bagasse at a reducing sugar concentration were achieved. Soluble metabolite product (SMP) was predominantly acetic acid indicating the acetate-type fermentation. Conclusions: The strain KKU-ED1 appeared to be a suitable candidate for thermophilic fermentative hydrogen production from hemicellulosic fraction of lignocellulosic materials due to its ability to use various types of carbon sources.

Nanqi Ren - One of the best experts on this subject based on the ideXlab platform.

  • alkali urea pretreatment of rice straw at low temperature for enhanced biological hydrogen production
    Bioresource Technology, 2018
    Co-Authors: Lili Dong, Lei Zhao, Guang-li Cao, Bing-feng Liu, Nanqi Ren
    Abstract:

    A pretreatment process using alkali/urea solution at low temperature was proposed for enhanced cellulosic biohydrogen production. Different alkaline solutions with both presence and absence of urea were studied. It can be found NaOH/Urea pretreatment exhibited excellent pretreatment performance at temperature from -8 °C to -20 °C. Microscopic structure observation combined FTIR analysis further demonstrated that NaOH/Urea pretreatment at low temperature could effectively disrupt the structure of rice straw and made more cellulose and hemicellulose available. The pretreated materials were then subjected for biohydrogen production by Thermoanaerobacterium thermosaccharolyticum M18. The maximum hydrogen production and energy conversion efficiency of 22.08 mmol/L and 9.76% were obtained from NaOH/Urea pretreated rice straw at low temperature. The results were 161.92% and 56.91% higher than the counterpart without pretreatment, respectively. This study provides a new direction to pretreat lignocellulose efficiently for enhanced biohydrogen production at cold climate region.

  • Consolidated bioprocessing performance of Thermoanaerobacterium thermosaccharolyticum M18 on fungal pretreated cornstalk for enhanced hydrogen production.
    Biotechnology for Biofuels, 2014
    Co-Authors: Lei Zhao, Guang-li Cao, Aijie Wang, Hong Yu Ren, Kun Zhang, Nanqi Ren
    Abstract:

    Background Biological hydrogen production from lignocellulosic biomass shows great potential as a promising alternative to conventional hydrogen production methods, such as electrolysis of water and coal gasification. Currently, most researches on biohydrogen production from lignocellulose concentrate on consolidated bioprocessing, which has the advantages of simpler operation and lower cost over processes featuring dedicated cellulase production. However, the recalcitrance of the lignin structure induces a low cellulase activity, making the carbohydrates in the hetero-matrix more unapproachable. Pretreatment of lignocellulosic biomass is consequently an extremely important step in the commercialization of biohydrogen, and for massive realization of lignocellulosic biomass as alternative fuel feedstock. Thus, development of a pretreatment method which is cost efficient, environmentally benign, and highly efficient for enhanced consolidated bioprocessing of lignocellulosic biomass to hydrogen is essential.

  • enzymatic saccharification of cornstalk by onsite cellulases produced by trichoderma viride for enhanced biohydrogen production
    Gcb Bioenergy, 2013
    Co-Authors: Lei Zhao, Guang-li Cao, Aijie Wang, Hong Yu Ren, Nanqi Ren
    Abstract:

    Lignocellulosic biomass, if properly saccharified, could be an ideal feedstock for biohydrogen production. However, the high cellulases cost is the key obstacle to its development. In this work, cost-effective enzyme produced by Trichoderma viride was used to saccharify cornstalk. To obtain high sugar yield, a central composite design of response surface method was used to optimize enzymatic saccharification process. Experimental results showed that the enzymatic saccharification rate reached the highest of 81.2% when pH, temperature, cellulases and substrate concentration were 5, 49.7 °C, 35.7 IU g � 1 , and 38.5 g L � 1 , respectively. The cornstalk hydrolysate was subsequently introduced to fermentation by Thermoanaerobacterium thermosaccharolyticum W16, the yield of hydrogen reached the highest level of 90.6 ml H2 g � 1 pretreated cornstalk. The present results indicate the potential of using T. thermosaccharolyticum W16 for high yield conversion of cornstalk hydrolysate, which was saccharified by onsite enzyme produced by T. viride.

  • Simultaneous saccharification and fermentation of fungal pretreated cornstalk for hydrogen production using Thermoanaerobacterium thermosaccharolyticum W16.
    Bioresource technology, 2013
    Co-Authors: Lei Zhao, Guang-li Cao, Aijie Wang, Hong Yu Ren, Wan-qian Guo, Nanqi Ren
    Abstract:

    Abstract In this research, environmentally friendly fungal pretreatment was first adopted for deconstruction of cornstalk. Then the fungal-pretreated cornstalk was employed to produce hydrogen in simultaneous saccharification and fermentation (SSF) using crude enzyme from Trichoderma viride and Thermoanaerobacterium thermosaccharolyticum W16. The influence of various factors including substrate concentration, initial pH, and enzyme loading on hydrogen production were evaluated. The highest hydrogen yield of 89.3 ml/g-cornstalk was obtained with an initial pH 6.5, 0.75% substrate concentration, and 34 FPU/g cellulose. Compared the result with SSF of physical or chemical pretreated lignocellulosic materials, this research suggested an economic and efficient way for hydrogen production from lignocellulosic biomass.

  • fungal pretreatment of cornstalk with phanerochaete chrysosporium for enhancing enzymatic saccharification and hydrogen production
    Bioresource Technology, 2012
    Co-Authors: Lei Zhao, Guang-li Cao, Aijie Wang, Hong Yu Ren, De Dong, Zinan Liu, Xiaoyu Guan, Nanqi Ren
    Abstract:

    Abstract The feasibility of fungal pretreatment of cornstalk with Phanerochaete chrysosporium for enzymatic saccharification and H2 production was investigated in this study. Firstly, cornstalk was pretreated with P. chrysosporium at 29 °C under static condition for 15 d, lignin reduction was up to 34.3% with holocellulose loss less than 10%. Microscopic structure observation combined FTIR analysis further demonstrated that the lignin and crystallinity were decreased. Subsequently, the fungal-pretreated cornstalk was subjected to enzymatic hydrolysis by the crude cellulase from Trichoderma viride to produce fermentable sugars which were then fermented to bio-H2 using Thermoanaerobacterium thermosaccharolyticum W16. The maximum enzymatic saccharification was found to be 47.3% which was 20.3% higher than the control without pretreatment. Upon fermentation of enzymatic hydrolysate, the yield of H2 was calculated to be 80.3 ml/g-pretreated cornstalk. The present results suggested the potential of using hydrogen-producing bacteria for high-yield conversion of cornstalk into bio-H2 integrate with biological pretreatment and enzymatic saccharification.

Sompong Othong - One of the best experts on this subject based on the ideXlab platform.

  • effects of volatile fatty acids in biohydrogen effluent on biohythane production from palm oil mill effluent under thermophilic condition
    Electronic Journal of Biotechnology, 2017
    Co-Authors: Chonticha Mamimin, Prawit Kongjan, P Prasertsan, Sompong Othong
    Abstract:

    Background: Biohydrogen effluent contains a high concentration of volatile fatty acid (VFA) mainly as butyric, acetic, lactic and propionic acids. The presence of various VFAs (mixture VFAs) and their cooperative effects on two-stage biohythane production need to be further studied. The effect of VFA concentrations in biohydrogen effluent of palm oil mill effluent (POME) on methane yield in methane stage of biohythane production was investigated. Results: The methane yield obtained in low VFA loading (0.9 and 1.8 g/L) was 15–20% times greater than that of high VFA loading (3.6 and 4.7 g/L). Butyric acid at high concentrations (8 g/L) has the individual significantly negative effect the methane production process ( P P Geobacillus sp., Thermoanaerobacterium thermosaccharolyticum , Methanoculleus thermophilus and Methanothermobacter delfuvii resulting in low methane yield. Conclusion: Preventing the high concentration of butyric acid, and propionic acid in the hydrogenic effluent could enhance methane production in two-stage anaerobic digestion for biohythane production. Normal 0 false false false ES-CL X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabla normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:8.0pt; mso-para-margin-left:0cm; line-height:107%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri",sans-serif; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi; mso-ansi-language:ES-CL;} Normal 0 false false false EN-US X-NONE TH

  • two stage thermophilic fermentation and mesophilic methanogen process for biohythane production from palm oil mill effluent
    International Journal of Hydrogen Energy, 2015
    Co-Authors: Chonticha Mamimin, Prawit Kongjan, P Prasertsan, Apinya Singkhala, Benjaporn Suraraksa, Tsuyoshi Imai, Sompong Othong
    Abstract:

    Abstract A two-stage thermophilic fermentation and mesophilic methanogenic process for biohythane production from palm oil mill effluent (POME) was investigated. The hydrogen and methane potential from POME were 170–200 L H 2  kgCOD −1 and 210–292 L CH 4  kgCOD −1 , respectively. A continuous two-stage process with hydraulic retention time (HRT) of 2 d for hydrogen reactor and 15 d for methane reactor, obtained 34% higher energy yield than single stage methane production. The hydrogen and methane yields from two-stage were 210 L H 2  kgCOD −1 and 315 L CH 4  kgCOD −1 , respectively with total energy yield of 15.34 MJ kgCOD −1 . Mixed hydrogen and methane (biohythane) production rate was 4.4 L biogas L −1 d −1 with containing of 51% CH 4 , 14% H 2 and 35% CO 2 . Hydrogen reactor was dominated with hydrogen producing bacteria of Thermoanaerobacterium thermosaccharolyticum , while acetoclastic Methanoculleus sp. was the dominant methanogen in methane reactor. Two-stage process for biohythane production could efficiently for energy recovery from POME.

  • effect of temperature and initial ph on biohydrogen production from palm oil mill effluent long term evaluation and microbial community analysis
    Electronic Journal of Biotechnology, 2011
    Co-Authors: Sompong Othong, Chonticha Mamimin, P Prasertsan
    Abstract:

    Anaerobic sludge from palm oil mill effluent (POME) treatment plant was used as a source of inocula for the conversion of POME into hydrogen. Optimization of temperature and initial pH for biohydrogen production from POME was investigated by response surface methodology. Temperature of 60oC and initial pHof 5.5 was optimized for anaerobic microflora which gave a maximum hydrogen production of 4820 ml H 2 /l-POME corresponding to hydrogen yield of 243 ml H 2 /g-sugar. Total sugar consumption and chemical oxygen demand (COD) removal efficiency were 98.7% and 46%, respectively. Long-term hydrogen production in continuous reactor at HRT of 2 days, 1 day and 12 hrs were 4850 ± 90, 4660 ± 99 and 2590 ± 120 ml H 2 /l-POME, respectively. Phylogenetic analysis of the mixed culture revealed that members involved hydrogen producers in both batch and continuous reactors were phylogenetically related to the Thermoanaerobacterium thermosaccharolyticum . Batch reactor showed more diversity of microorganisms than continuous reactor. Microbial community structure of batch reactor was comprised of T. thermosaccharolyticum , T. bryantii, Thermoanaerobacterium sp., Clostridium thermopalmarium and Clostridium NS5-4, while continuous reactor was comprised of T. thermosaccharolyticum , T. bryantii and Thermoanaerobacterium sp. POME is good substrate for biohydrogen production under thermophilic condition with Thermoanaerobacterium species play an important role in hydrogen fermentation.

  • optimization and microbial community analysis for production of biohydrogen from palm oil mill effluent by thermophilic fermentative process
    International Journal of Hydrogen Energy, 2009
    Co-Authors: P Prasertsan, Sompong Othong, Nilskare Birkeland
    Abstract:

    Abstract The optimum values of hydraulic retention time (HRT) and organic loading rate (OLR) of an anaerobic sequencing batch reactor (ASBR) for biohydrogen production from palm oil mill effluent (POME) under thermophilic conditions (60 °C) were investigated in order to achieve the maximum process stability. Microbial community structure dynamics in the ASBR was studied by denaturing gradient gel electrophoresis (DGGE) aiming at improved insight into the hydrogen fermentation microorganisms. The optimum values of 2-d HRT with an OLR of 60 gCOD l −1  d −1 gave a maximum hydrogen yield of 0.27 l H 2  g COD −1 with a volumetric hydrogen production rate of 9.1 l H 2  l −1  d −1 (16.9 mmol l −1  h −1 ). The hydrogen content, total carbohydrate consumption, COD (chemical oxygen demand) removal and suspended solids removal were 55 ± 3.5%, 92 ± 3%, 57 ± 2.5% and 78 ± 2%, respectively. Acetic acid and butyric acid were the major soluble end-products. The microbial community structure was strongly dependent on the HRT and OLR. DGGE profiling illustrated that Thermoanaerobacterium spp., such as Thermoanaerobacterium thermosaccharolyticum and Thermoanaerobacterium bryantii , were dominant and probably played an important role in hydrogen production under the optimum conditions. The shift in the microbial community from a dominance of T. thermosaccharolyticum to a community where also Caloramator proteoclasticus constituted a major component occurred at suboptimal HRT (1 d) and OLR (80 gCOD l −1  d −1 ) conditions. The results showed that the hydrogen production performance was closely correlated with the bacterial community structure. This is the first report of a successful ASBR operation achieving a high hydrogen production rate from real wastewater (POME).

  • optimization and microbial community analysis for production of biohydrogen from palm oil mill effluent by thermophilic fermentative process
    International Journal of Hydrogen Energy, 2009
    Co-Authors: P Prasertsan, Sompong Othong, Nilskare Birkeland
    Abstract:

    Abstract The optimum values of hydraulic retention time (HRT) and organic loading rate (OLR) of an anaerobic sequencing batch reactor (ASBR) for biohydrogen production from palm oil mill effluent (POME) under thermophilic conditions (60 °C) were investigated in order to achieve the maximum process stability. Microbial community structure dynamics in the ASBR was studied by denaturing gradient gel electrophoresis (DGGE) aiming at improved insight into the hydrogen fermentation microorganisms. The optimum values of 2-d HRT with an OLR of 60 gCOD l −1  d −1 gave a maximum hydrogen yield of 0.27 l H 2  g COD −1 with a volumetric hydrogen production rate of 9.1 l H 2  l −1  d −1 (16.9 mmol l −1  h −1 ). The hydrogen content, total carbohydrate consumption, COD (chemical oxygen demand) removal and suspended solids removal were 55 ± 3.5%, 92 ± 3%, 57 ± 2.5% and 78 ± 2%, respectively. Acetic acid and butyric acid were the major soluble end-products. The microbial community structure was strongly dependent on the HRT and OLR. DGGE profiling illustrated that Thermoanaerobacterium spp., such as Thermoanaerobacterium thermosaccharolyticum and Thermoanaerobacterium bryantii , were dominant and probably played an important role in hydrogen production under the optimum conditions. The shift in the microbial community from a dominance of T. thermosaccharolyticum to a community where also Caloramator proteoclasticus constituted a major component occurred at suboptimal HRT (1 d) and OLR (80 gCOD l −1  d −1 ) conditions. The results showed that the hydrogen production performance was closely correlated with the bacterial community structure. This is the first report of a successful ASBR operation achieving a high hydrogen production rate from real wastewater (POME).

P Prasertsan - One of the best experts on this subject based on the ideXlab platform.

  • two stage thermophilic bio hydrogen and methane production from oil palm trunk hydrolysate using Thermoanaerobacterium thermosaccharolyticum kku19
    International Journal of Hydrogen Energy, 2017
    Co-Authors: Saruda Sitthikitpanya, Alissara Reungsang, P Prasertsan, Samir Kumar Khanal
    Abstract:

    Abstract The two-stage process for thermophilic bio-hydrogen production followed by methane production was examined on a hydrolysate obtained from oil palm trunk (OPT). The optimum conditions for lime pretreatment and enzymatic hydrolysis of OPT was a lime loading of 0.2 g Ca(OH)2/g-OPT, pretreatment time of 60 min, temperature of 121 °C and enzyme loading of 35 filter paper units/g-OPT. A maximum total reducing sugar yield of 473 mg/g-OPT was obtained, which was 2.7-fold higher than that of untreated OPT. The OPT hydrolysate was used as a substrate for bio-hydrogen production by Thermoanaerobacterium thermosaccharolyticum KKU19 in the first stage. The maximum hydrogen production potential of 2179 mL H2/Lsubstrate was obtained under the optimum conditions of 16.5 g/L initial substrate concentration, initial pH of 6.7 and 54.5 °C, respectively. Acidic effluent was used to produce methane in the second stage in which the methane yield of 272.4 mL/g-chemical oxygen demand (COD) was achieved. The two-stage hydrogen and methane production resulted in energy yield of 10.6 kJ/g-CODadded with 83% COD removal.

  • effects of volatile fatty acids in biohydrogen effluent on biohythane production from palm oil mill effluent under thermophilic condition
    Electronic Journal of Biotechnology, 2017
    Co-Authors: Chonticha Mamimin, Prawit Kongjan, P Prasertsan, Sompong Othong
    Abstract:

    Background: Biohydrogen effluent contains a high concentration of volatile fatty acid (VFA) mainly as butyric, acetic, lactic and propionic acids. The presence of various VFAs (mixture VFAs) and their cooperative effects on two-stage biohythane production need to be further studied. The effect of VFA concentrations in biohydrogen effluent of palm oil mill effluent (POME) on methane yield in methane stage of biohythane production was investigated. Results: The methane yield obtained in low VFA loading (0.9 and 1.8 g/L) was 15–20% times greater than that of high VFA loading (3.6 and 4.7 g/L). Butyric acid at high concentrations (8 g/L) has the individual significantly negative effect the methane production process ( P P Geobacillus sp., Thermoanaerobacterium thermosaccharolyticum , Methanoculleus thermophilus and Methanothermobacter delfuvii resulting in low methane yield. Conclusion: Preventing the high concentration of butyric acid, and propionic acid in the hydrogenic effluent could enhance methane production in two-stage anaerobic digestion for biohythane production. Normal 0 false false false ES-CL X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabla normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:8.0pt; mso-para-margin-left:0cm; line-height:107%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri",sans-serif; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi; mso-ansi-language:ES-CL;} Normal 0 false false false EN-US X-NONE TH

  • two stage thermophilic fermentation and mesophilic methanogen process for biohythane production from palm oil mill effluent
    International Journal of Hydrogen Energy, 2015
    Co-Authors: Chonticha Mamimin, Prawit Kongjan, P Prasertsan, Apinya Singkhala, Benjaporn Suraraksa, Tsuyoshi Imai, Sompong Othong
    Abstract:

    Abstract A two-stage thermophilic fermentation and mesophilic methanogenic process for biohythane production from palm oil mill effluent (POME) was investigated. The hydrogen and methane potential from POME were 170–200 L H 2  kgCOD −1 and 210–292 L CH 4  kgCOD −1 , respectively. A continuous two-stage process with hydraulic retention time (HRT) of 2 d for hydrogen reactor and 15 d for methane reactor, obtained 34% higher energy yield than single stage methane production. The hydrogen and methane yields from two-stage were 210 L H 2  kgCOD −1 and 315 L CH 4  kgCOD −1 , respectively with total energy yield of 15.34 MJ kgCOD −1 . Mixed hydrogen and methane (biohythane) production rate was 4.4 L biogas L −1 d −1 with containing of 51% CH 4 , 14% H 2 and 35% CO 2 . Hydrogen reactor was dominated with hydrogen producing bacteria of Thermoanaerobacterium thermosaccharolyticum , while acetoclastic Methanoculleus sp. was the dominant methanogen in methane reactor. Two-stage process for biohythane production could efficiently for energy recovery from POME.

  • effect of temperature and initial ph on biohydrogen production from palm oil mill effluent long term evaluation and microbial community analysis
    Electronic Journal of Biotechnology, 2011
    Co-Authors: Sompong Othong, Chonticha Mamimin, P Prasertsan
    Abstract:

    Anaerobic sludge from palm oil mill effluent (POME) treatment plant was used as a source of inocula for the conversion of POME into hydrogen. Optimization of temperature and initial pH for biohydrogen production from POME was investigated by response surface methodology. Temperature of 60oC and initial pHof 5.5 was optimized for anaerobic microflora which gave a maximum hydrogen production of 4820 ml H 2 /l-POME corresponding to hydrogen yield of 243 ml H 2 /g-sugar. Total sugar consumption and chemical oxygen demand (COD) removal efficiency were 98.7% and 46%, respectively. Long-term hydrogen production in continuous reactor at HRT of 2 days, 1 day and 12 hrs were 4850 ± 90, 4660 ± 99 and 2590 ± 120 ml H 2 /l-POME, respectively. Phylogenetic analysis of the mixed culture revealed that members involved hydrogen producers in both batch and continuous reactors were phylogenetically related to the Thermoanaerobacterium thermosaccharolyticum . Batch reactor showed more diversity of microorganisms than continuous reactor. Microbial community structure of batch reactor was comprised of T. thermosaccharolyticum , T. bryantii, Thermoanaerobacterium sp., Clostridium thermopalmarium and Clostridium NS5-4, while continuous reactor was comprised of T. thermosaccharolyticum , T. bryantii and Thermoanaerobacterium sp. POME is good substrate for biohydrogen production under thermophilic condition with Thermoanaerobacterium species play an important role in hydrogen fermentation.

  • optimization and microbial community analysis for production of biohydrogen from palm oil mill effluent by thermophilic fermentative process
    International Journal of Hydrogen Energy, 2009
    Co-Authors: P Prasertsan, Sompong Othong, Nilskare Birkeland
    Abstract:

    Abstract The optimum values of hydraulic retention time (HRT) and organic loading rate (OLR) of an anaerobic sequencing batch reactor (ASBR) for biohydrogen production from palm oil mill effluent (POME) under thermophilic conditions (60 °C) were investigated in order to achieve the maximum process stability. Microbial community structure dynamics in the ASBR was studied by denaturing gradient gel electrophoresis (DGGE) aiming at improved insight into the hydrogen fermentation microorganisms. The optimum values of 2-d HRT with an OLR of 60 gCOD l −1  d −1 gave a maximum hydrogen yield of 0.27 l H 2  g COD −1 with a volumetric hydrogen production rate of 9.1 l H 2  l −1  d −1 (16.9 mmol l −1  h −1 ). The hydrogen content, total carbohydrate consumption, COD (chemical oxygen demand) removal and suspended solids removal were 55 ± 3.5%, 92 ± 3%, 57 ± 2.5% and 78 ± 2%, respectively. Acetic acid and butyric acid were the major soluble end-products. The microbial community structure was strongly dependent on the HRT and OLR. DGGE profiling illustrated that Thermoanaerobacterium spp., such as Thermoanaerobacterium thermosaccharolyticum and Thermoanaerobacterium bryantii , were dominant and probably played an important role in hydrogen production under the optimum conditions. The shift in the microbial community from a dominance of T. thermosaccharolyticum to a community where also Caloramator proteoclasticus constituted a major component occurred at suboptimal HRT (1 d) and OLR (80 gCOD l −1  d −1 ) conditions. The results showed that the hydrogen production performance was closely correlated with the bacterial community structure. This is the first report of a successful ASBR operation achieving a high hydrogen production rate from real wastewater (POME).

Irini Angelidaki - One of the best experts on this subject based on the ideXlab platform.

  • enhancement of bioenergy production from organic wastes by two stage anaerobic hydrogen and methane production process
    Bioresource Technology, 2011
    Co-Authors: Gang Luo, Qi Zhou, Li Xie, Irini Angelidaki
    Abstract:

    Abstract The present study investigated a two-stage anaerobic hydrogen and methane process for increasing bioenergy production from organic wastes. A two-stage process with hydraulic retention time (HRT) 3 d for hydrogen reactor and 12 d for methane reactor, obtained 11% higher energy compared to a single-stage methanogenic process (HRT 15 d) under organic loading rate (OLR) 3 gVS/(L d). The two-stage process was still stable when the OLR was increased to 4.5 gVS/(L d), while the single-stage process failed. The study further revealed that by changing the HRThydrogen:HRTmethane ratio of the two-stage process from 3:12 to 1:14, 6.7%, more energy could be obtained. Microbial community analysis indicated that the dominant bacterial species were different in the hydrogen reactors (Thermoanaerobacterium thermosaccharolyticum-like species) and methane reactors (Clostridium thermocellum-like species). The changes of substrates and HRT did not change the dominant species. The archaeal community structures in methane reactors were similar both in single- and two- stage reactors, with acetoclastic methanogens Methanosarcina acetivorans-like organisms as the dominant species.

  • long term effect of inoculum pretreatment on fermentative hydrogen production by repeated batch cultivations homoacetogenesis and methanogenesis as competitors to hydrogen production
    Biotechnology and Bioengineering, 2011
    Co-Authors: Dimitar Borisov Karakashev, Qi Zhou, Irini Angelidaki
    Abstract:

    : Long-term effects of inoculum pretreatments (heat, acid, loading-shock) on hydrogen production from glucose under different temperatures (37 °C, 55 °C) and initial pH (7 and 5.5) were studied by repeated batch cultivations. Results obtained showed that it was necessary to investigate the long-term effect of inoculum pretreatment on hydrogen production since pretreatments may just temporarily inhibit the hydrogen consuming processes. After long-term cultivation, pretreated inocula did not enhance hydrogen production compared to untreated inocula under mesophilic conditions (initial pH 7 and pH 5.5) and thermophilic conditions (initial pH 7). However, pretreatment could inhibit lactate production and lead to higher hydrogen yield under thermophilic conditions at initial pH 5.5. The results further demonstrated that inoculum pretreatment could not permanently inhibit either methanogenesis or homoacetogenesis, and methanogenesis and homoacetogenesis could only be inhibited by proper control of fermentation pH and temperature. Methanogenic activity could be inhibited at pH lower than 6, both under mesophilic and thermophilic conditions, while homoacetogenic activity could only be inhibited under thermophilic condition at initial pH 5.5. Microbial community analysis showed that pretreatment did not affect the dominant bacteria. The dominant bacteria were Clostridium butyricum related organisms under mesophilic condition (initial pH 7 and 5.5), Thermoanaerobacterium sp. related organisms under thermophilic condition (initial pH 7), and Thermoanaerobacterium thermosaccharolyticum related organisms under thermophilic condition (initial pH 5.5). Results from this study clearly indicated that the long-term effects of inoculum pretreatments on hydrogen production, methanogenesis, homoacetogenesis and dominant bacteria were dependent on fermentation temperature and pH.

  • Biohydrogen production from wheat straw hydrolysate by dark fermentation using extreme thermophilic mixed culture
    Biotechnology and Bioengineering, 2010
    Co-Authors: Prawit Kongjan, Meher Kotay, Sompong O-thong, Booki Min, Irini Angelidaki
    Abstract:

    Hydrolysate was tested as substrate for hydrogen production by extreme thermophilic mixed culture (70 degrees C) in both batch and continuously fed reactors. Hydrogen was produced at hydrolysate concentrations up to 25% (v/v), while no hydrogen was produced at hydrolysate concentration of 30% (v/v), indicating that hydrolysate at high concentrations was inhibiting the hydrogen fermentation process. In addition, the lag phase for hydrogen production was strongly influenced by the hydrolysate concentration, and was prolonged from approximately 11 h at the hydrolysate concentrations below 20% (v/v) to 38 h at the hydrolysate concentration of 25% (v/v). The maximum hydrogen yield as determined in batch assays was 318.4 +/- 5.2 mL-H(2)/g-sugars (14.2 +/- 0.2 mmol-H(2)/g-sugars) at the hydrolysate concentration of 5% (v/v). Continuously fed, and the continuously stirred tank reactor (CSTR), operating at 3 day hydraulic retention time (HRT) and fed with 20% (v/v) hydrolysate could successfully produce hydrogen. The hydrogen yield and production rate were 178.0 +/- 10.1 mL-H(2)/g-sugars (7.9 +/- 0.4 mmol H(2)/g-sugars) and 184.0 +/- 10.7 mL-H(2)/day L(reactor) (8.2 +/- 0.5 mmol-H(2)/day L(reactor)), respectively, corresponding to 12% of the chemical oxygen demand (COD) from sugars. Additionally, it was found that toxic compounds, furfural and hydroxymethylfurfural (HMF), contained in the hydrolysate were effectively degraded in the CSTR, and their concentrations were reduced from 50 and 28 mg/L, respectively, to undetectable concentrations in the effluent. Phylogenetic analysis of the mixed culture revealed that members involved hydrogen producers in both batch and CSTR reactors were phylogenetically related to the Caldanaerobacter subteraneus, Thermoanaerobacter subteraneus, and Thermoanaerobacterium thermosaccharolyticum.

  • high rate continuous hydrogen production by Thermoanaerobacterium thermosaccharolyticum psu 2 immobilized on heat pretreated methanogenic granules
    International Journal of Hydrogen Energy, 2008
    Co-Authors: P Prasertsan, Sompong Othong, Dimitar Borisov Karakashev, Irini Angelidaki
    Abstract:

    Abstract Biohydrogen production from Thermoanaerobacterium thermosaccharolyticum strain PSU-2 was examined in upflow anaerobic sludge blanket (UASB) reactor and carrier-free upflow anaerobic reactor (UA), both fed with sucrose and operating at 60 °C. Heat-pretreated methanogenic granules were used as carrier to immobilize T. thermosaccharolyticum strain PSU-2 in UASB reactor operated at a hydraulic retention time (HRT) ranging from 0.75 to 24 h and corresponding sucrose loading rate from 58.5 to 2.4 mmol sucrose l−1 h−1. In comparison with hydrogen production rate of 12.1 mmol H2 l−1 h−1 obtained by carrier-free reactor upflow anaerobic (UA) system, a greatly improved hydrogen production rate up to 152 mmol H2 l−1 h−1 was demonstrated by the granular cells in UASB system. The biofilm of T. thermosaccharolyticum strain PSU-2 developed on treated methanogenic granules in UASB reactor substantially enhanced biomass retention (3 times), and production of hydrogen (12 times) compared to carrier-free reactor. It appears to be the most preferred process for highly efficient dark fermentative hydrogen production from sugar containing wastewater under thermophilic conditions.

  • thermophilic fermentative hydrogen production by the newly isolated Thermoanaerobacterium thermosaccharolyticum psu 2
    International Journal of Hydrogen Energy, 2008
    Co-Authors: Sompong Othong, P Prasertsan, Dimitar Borisov Karakashev, Irini Angelidaki
    Abstract:

    Abstract A thermophilic H 2 -producing bacterial strain was isolated from a biohydrogen reactor fed with palm oil mill effluent (POME) and identified as Thermoanaerobacterium thermosaccharolyticum using 16S rRNA gene analysis. The isolated bacterium, designated as T. thermosaccharolyticum PSU-2, showed a high yield and production rate of H 2 . Temperature optimum, pH optimum and substrate utilization for H 2 production were investigated in batch conditions. All of tested substrate was utilized for H 2 production, while sucrose, xylose and starch were the preferred substrates. The strain produced H 2 within a wide range of pH (4.5–8) and temperature (45– 70 ∘ C ), with the optimal temperature 60 ∘ C and optimal initial pH about 6.25. Maximum of H 2 production rate was registered from hour 8 to hour 16 in late exponential phase. The H 2 production was drastically reduced in a prolonged fermentation (24 h) and stopped at pH 4.5 due to the accumulation of organic acids. The maximum H 2 production yield and rate at sucrose concentration of 20 g l - 1 , pH 6.25 and temperature 60 ∘ C were 2.53 mol H 2 mol - 1 hexose and 12.12 mmol H 2 l - 1 h - 1 , respectively. Organic nitrogen amended medium improved the H 2 production with 68% compared to inorganic nitrogen amended medium. The strain performed ethanol–acetate type fermentation in inorganic nitrogen amended medium, while it performed butyrate–acetate type fermentation in organic nitrogen amended medium.