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Michael Goodfellow - One of the best experts on this subject based on the ideXlab platform.
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Amycolatopsis granulosa sp. nov., Amycolatopsis ruanii sp. nov. and Amycolatopsis thermalba sp. nov., Thermophilic Actinomycetes isolated from arid soils.
International journal of systematic and evolutionary microbiology, 2011Co-Authors: Tiago Domingues Zucchi, Geok Yuan Annie Tan, Avinash Naga Venkata Bonda, Sarah Frank, Jenileima Devi Kshetrimayum, Michael GoodfellowAbstract:The taxonomic positions of three Thermophilic Actinomycetes isolated from arid soil samples were established by using a polyphasic approach. The organisms had chemical and morphological features that were consistent with their classification in the genus Amycolatopsis. 16S rRNA gene sequence data supported the classification of the isolates in the genus Amycolatopsis and showed that they formed distinct branches in the Amycolatopsis methanolica subclade. DNA-DNA relatedness studies between the isolates and their phylogenetic neighbours showed that they belonged to distinct genomic species. The three isolates were readily distinguished from one another and from the type strains of species classified in the A. methanolica subclade based on a combination of phenotypic properties and by genomic fingerprinting. Consequently, it is proposed that the three isolates be classified in the genus Amycolatopsis as representatives of Amycolatopsis granulosa sp. nov. (type strain GY307(T) = NCIMB 14709(T) = NRRL B-24844(T)), Amycolatopsis ruanii sp. nov. (type strain NMG112(T) = NCIMB 14711(T) = NRRL B-24848(T)) and Amycolatopsis thermalba sp. nov. (type strain SF45(T) = NCIMB 14705(T) = NRRL B-24845(T)).
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Amycolatopsis thermophila sp. nov. and Amycolatopsis viridis sp. nov., Thermophilic Actinomycetes isolated from arid soil.
International Journal of Systematic and Evolutionary Microbiology, 2011Co-Authors: Tiago Domingues Zucchi, Geok Yuan Annie Tan, Michael GoodfellowAbstract:The taxonomic positions of two Thermophilic Actinomycetes isolated from an arid Australian soil sample were established based on an investigation using a polyphasic taxonomic approach. The organisms had chemical and morphological properties typical of members of the genus Amycolatopsis and formed distinct phyletic lines in the Amycolatopsis methanolica 16S rRNA subclade. The two organisms were distinguished from one another and from the type strains of related species of the genus Amycolatopsis using a range of phenotypic properties. Based on the combined genotypic and phenotypic data, it is proposed that the two isolates be classified in the genus Amycolatopsis as Amycolatopsis thermophila sp. nov. (type strain GY088 T 5NCIMB 14699 T 5NRRL B-24836 T ) and Amycolatopsis viridis sp. nov. (type strain GY115 T 5NCIMB 14700 T 5NRRL B-24837 T ).
Goodfellow Michael - One of the best experts on this subject based on the ideXlab platform.
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Amycolatopsis thermophila sp nov and Amycolatopsis viridis sp nov., Thermophilic Actinomycetes isolated from arid soil
READING, 2012Co-Authors: Zucchi, Tiago Domingues, Tan, Geok Yuan Annie, Goodfellow MichaelAbstract:The taxonomic positions of two Thermophilic Actinomycetes isolated from an arid Australian soil sample were established based on an investigation using a polyphasic taxonomic approach. The organisms had chemical and morphological properties typical of members of the genus Amycolatopsis and formed distinct phyletic lines in the Amycolatopsis methanolica 16S rRNA subclade. The two organisms were distinguished from one another and from the type strains of related species of the genus Amycolatopsis using a range of phenotypic properties. Based on the combined genotypic and phenotypic data, it is proposed that the two isolates be classified in the genus Amycolatopsis as Amycolatopsis thermophila sp. nov. (type strain GY088(T)=NCIMB 14699(T)=NRRL B-24836(T)) and Amycolatopsis viridis sp. nov. (type strain GY115(T)=NCIMB 14700(T)= NRRL B-24837(T)).Conselho Nacional de Desenvolvimento Cientifico e Tecnologico in the UK [201066/2009-2
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Amycolatopsis granulosa sp nov., Amycolatopsis ruanii sp nov and Amycolatopsis thermalba sp nov., Thermophilic Actinomycetes isolated from arid soils
READING, 2012Co-Authors: Zucchi, Tiago Domingues, Tan, Geok Yuan Annie, Bonda, Avinash Naga Venkata, Frank Sarah, Kshetrimayum, Jenileima Devi, Goodfellow MichaelAbstract:The taxonomic positions of three Thermophilic Actinomycetes isolated from arid soil samples were established by using a polyphasic approach. The organisms had chemical and morphological features that were consistent with their classification in the genus Amycolatopsis. 16S rRNA gene sequence data supported the classification of the isolates in the genus Amycolatopsis and showed that they formed distinct branches in the Amycolatopsis methanolica subclade. DNA-DNA relatedness studies between the isolates and their phylogenetic neighbours showed that they belonged to distinct genomic species. The three isolates were readily distinguished from one another and from the type strains of species classified in the A. methanolica subclade based on a combination of phenotypic properties and by genomic fingerprinting. Consequently, it is proposed that the three isolates be classified in the genus Amycolatopsis as representatives of Amycolatopsis granulosa sp. nov. (type strain GY307(T)=NCIMB 14709(T)=NRRL B-24844(T)), Amycolatopsis ruanii sp. nov. (type strain NMG112(T)=NCIMB 14711(T)=NRRL B-24848(T)) and Amycolatopsis thermalba sp. nov. (type strain SF45(T)=NCIMB 14705(T)=NRRL B-24845(T)).Conselho Nacional de Desenvolvimento Cientifico e Tecnologico [201066/2009-2
Peter Kämpfer - One of the best experts on this subject based on the ideXlab platform.
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recommendations for study design and sampling strategies for airborne microorganisms mvoc and odours in the surrounding of composting facilities
International Journal of Hygiene and Environmental Health, 2008Co-Authors: Andreas Albrecht, Gefion Brunnemannstubbe, Guido Fischer, Udo Jäckel, Peter KämpferAbstract:Abstract Microorganisms and odour emissions from composting plants often lead to complaints by residents, especially by people living close to such plants. Both parameters were studied in a systematic approach under specific local meteorological conditions at nine different composting plants in Germany with emphasis on dispersal of microorganisms. Measurements were done at emission points and at sampling sites in the downwind and upwind directions of the facilities under ‘normal case’ (i.e. weather conditions typical for the location in combination with working activities at the plants) and ‘real worst case’ conditions (dispersal of bioaerosols into the surroundings expected to occur with high probability). Airborne microorganisms were sampled using filtration and impingement. Subsequent cultivation on four different culture media allowed quantification and identification of the culturable microflora. It turned out that a general assessment of emissions and dispersal of bioaerosols from composting plants is not possible because of the coherences of various factors influencing the dispersal. The site-specific meteorological situations must be considered carefully, whenever sampling locations are selected and need to be recorded in any sampling protocol. Air inversions in particular can lead to high concentrations of microorganisms (>104–105 cfu m−3 of Thermophilic Actinomycetes and thermotolerant fungi) in the surroundings of composting plants. Finally, it was shown that both thermotolerant fungi and Thermophilic Actinomycetes can serve as indicator organisms.
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analysis of airborne microorganisms mvoc and odour in the surrounding of composting facilities and implications for future investigations
International Journal of Hygiene and Environmental Health, 2008Co-Authors: Guido Fischer, Udo Jäckel, Andreas Albrecht, Peter KämpferAbstract:Abstract Emission and dispersal of microorganisms and odours from composting facilities were studied in a 3-year project at nine different composting facilities in Germany. Measurements were carried out under so-called ‘normal-case’, i.e. typical local climate conditions and working activities within the facilities, and ‘real worst-case’ conditions (‘drainage flow’ conditions) being characterized by the translocation of cold air mostly at night, and containing large amounts of bioaerosols. Highest concentrations of microorganisms were observed during turning of compost with a maximum of 2.4×106 cfu m−3 for Thermophilic Actinomycetes. Other groups of microorganisms were detected in concentrations of about 105 cfu m−3. During shredding of fresh organic material, the concentrations of all microorganisms reached 104 cfu m−3. Here, odour concentrations turned out to be highest (up to 1,367 odour units (OU) m−3). At facilities equipped with a biofilter (odour reduction), a decrease in OU by a factor of 10 was observed. In the surrounding of the facilities, highest concentrations ranged between 101–103 cfu m−3 upwind and from 101–104 cfu m−3 downwind. The specific local meteorological situations must be considered carefully in advance and during sampling. Especially ‘drainage flow’ situations can lead to high microorganism concentrations (>104–105 cfu m−3 of Thermophilic Actinomycetes and Thermophilic fungi) in the surroundings of composting facilities.
Chao Hsun Yang - One of the best experts on this subject based on the ideXlab platform.
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Production and Antioxidant Properties of the Ferulic Acid-Rich Destarched Wheat Bran Hydrolysate by Feruloyl Esterases from Thermophilic Actinomycetes
Bioresources, 2013Co-Authors: Yu-chun Huang, Cheng Yu Chen, Wei-lin Chen, Yong-ping Ciou, Ting-ya Yang, Chao Hsun YangAbstract:Ferulic acid is present at relatively high concentrations in the cell walls of several plants. Agricultural lignocelluloses are now used as bioresources in industry. This study attempted to increase the free ferulic acid content present in lignocellulose by using thermostable esterase produced from Thermophilic Actinomycetes to hydrolyze ester bonds. Destarched wheat bran was used as a carbon source for the production of esterases from the newly isolated Thermophilic Actinomycetes species Thermobifida fusca. After 96 h of cultivation, the esterase activity that accumulated in the culture broth was 946.0 U/mL. Two percent of the destarched wheat bran was then hydrolyzed by crude esterase preparation for 16 h. The ferulic acid was accumulated in the culture broth at a concentration of 310.0 M. The hydrolysate had better radical-scavenging ability for both 1,1-diphenyl-2-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical-scavenging ability, as well as reducing power than ferulic acid. These results showed that the ferulic acid-rich destarched wheat bran hydrolysate had good antioxidant properties. It is suggested that this process can be advantageous for the industrial production of antioxidants derived from agricultural bioresources.
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Properties of the newly isolated extracellular thermo-alkali-stable laccase from Thermophilic Actinomycetes, Thermobifida fusca and its application in dye intermediates oxidation
AMB Express, 2013Co-Authors: Cheng Yu Chen, Menghsiao Meng, Chien Mei Wei, Yu-chun Huang, Wen-hsiung Liu, Chao Hsun YangAbstract:Laccases are diphenol oxidases that have numerous applications to biotechnological processes. In this study, the laccase was produced from the Thermophilic Actinomycetes, Thermobifida fusca BCRC 19214. After 36 h of fermentation in a 5-liter fermentor, the culture broth accumulated 4.96 U/ml laccase activity. The laccase was purified 4.64-fold as measured by specific activity from crude culture filtrate by ultrafiltration concentration, Q-Sepharose FF and Sephacryl™ S-200 column chromatography. The overall yield of the purified enzyme was 7.49%. The molecular mass of purified enzyme as estimated by SDS-PAGE and by gel filtration on Sephacryl™ S-200 was found to be 73.3 kDa and 24.7 kDa, respectively, indicating that the laccase from T. fusca BCRC 19214 is a trimer. The internal amino acid sequences of the purified laccase, as determined by LC-MS/MS, had high homology with a superoxide dismutase from T. fusca YX. Approximately 95% of the original activity remained after treatment at 50°C for 3 h. and approximately 75% of the original activity remained after treatment at pH 10.0 for 24 h. This laccase could oxidize dye intermediates, especially 2,6-dimethylphenylalanine and p-aminophenol, to produce coloring. This is the first report on laccase properties from Thermophilic Actinomycetes. These properties suggest that this newly isolated laccase has potential for specific industrial applications.
Tiago Domingues Zucchi - One of the best experts on this subject based on the ideXlab platform.
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Amycolatopsis granulosa sp. nov., Amycolatopsis ruanii sp. nov. and Amycolatopsis thermalba sp. nov., Thermophilic Actinomycetes isolated from arid soils.
International journal of systematic and evolutionary microbiology, 2011Co-Authors: Tiago Domingues Zucchi, Geok Yuan Annie Tan, Avinash Naga Venkata Bonda, Sarah Frank, Jenileima Devi Kshetrimayum, Michael GoodfellowAbstract:The taxonomic positions of three Thermophilic Actinomycetes isolated from arid soil samples were established by using a polyphasic approach. The organisms had chemical and morphological features that were consistent with their classification in the genus Amycolatopsis. 16S rRNA gene sequence data supported the classification of the isolates in the genus Amycolatopsis and showed that they formed distinct branches in the Amycolatopsis methanolica subclade. DNA-DNA relatedness studies between the isolates and their phylogenetic neighbours showed that they belonged to distinct genomic species. The three isolates were readily distinguished from one another and from the type strains of species classified in the A. methanolica subclade based on a combination of phenotypic properties and by genomic fingerprinting. Consequently, it is proposed that the three isolates be classified in the genus Amycolatopsis as representatives of Amycolatopsis granulosa sp. nov. (type strain GY307(T) = NCIMB 14709(T) = NRRL B-24844(T)), Amycolatopsis ruanii sp. nov. (type strain NMG112(T) = NCIMB 14711(T) = NRRL B-24848(T)) and Amycolatopsis thermalba sp. nov. (type strain SF45(T) = NCIMB 14705(T) = NRRL B-24845(T)).
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Amycolatopsis thermophila sp. nov. and Amycolatopsis viridis sp. nov., Thermophilic Actinomycetes isolated from arid soil.
International Journal of Systematic and Evolutionary Microbiology, 2011Co-Authors: Tiago Domingues Zucchi, Geok Yuan Annie Tan, Michael GoodfellowAbstract:The taxonomic positions of two Thermophilic Actinomycetes isolated from an arid Australian soil sample were established based on an investigation using a polyphasic taxonomic approach. The organisms had chemical and morphological properties typical of members of the genus Amycolatopsis and formed distinct phyletic lines in the Amycolatopsis methanolica 16S rRNA subclade. The two organisms were distinguished from one another and from the type strains of related species of the genus Amycolatopsis using a range of phenotypic properties. Based on the combined genotypic and phenotypic data, it is proposed that the two isolates be classified in the genus Amycolatopsis as Amycolatopsis thermophila sp. nov. (type strain GY088 T 5NCIMB 14699 T 5NRRL B-24836 T ) and Amycolatopsis viridis sp. nov. (type strain GY115 T 5NCIMB 14700 T 5NRRL B-24837 T ).
