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Méndez Gabriela - One of the best experts on this subject based on the ideXlab platform.
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BIODEGRADACIÓN DE LOS FENOLES PRESENTES EN EL EXTRACTO DE GUARANGO(Caesalpinia Spinosa) Y EN LOS COLIRANTES ÍNDIGO CARMÍN, NARANJA II Y ROJO FENOL A TRAVÉZ DE Trametes Versicolor AND Aspergillus Niger.
2014Co-Authors: Huachi Laura, Macas, Ángela Macas, Méndez GabrielaAbstract:Esta investigación se la realizó con el fin de evaluar la capacidad de biodegradación de los fenoles presentes en el extracto de tánico de guarango((Caesalpina spinosa) y en los colorantes índigo carmín, naranja II y el rojo fenol, presentes en los desechos de la industria textil. Se utilizaron dos tipos de hongos, el de podredumbr blanca Trametes Versicolor y filamentos Aspergillus niger : Los tratamientos bológicos se aplicaron por triplicado, reaoizados por el método expectrofotométrico Uv por 15 días; los resultados obtenidos al aplicar el hongo Trametes Versicolor en el extracto titáico fue del 69.45% de degradación; y en el colorante índigo carmín fue del 100% de decoloración. Los resultados de la degradación de Trametes Versicolor y Aspergillus niger sobre el colorante rojo fenol se obtuvieron porcentajes de decoloración no mayores al 26%. Los hongos aplicados en los tratamientos biológicos presentaron tolerancia tanto a la composición del extracto tánico como a las concentraciones de cada uno de los colorantes usados, esto fue comprobado con la cinética de crecimiento para cada hongo.The research was done to evaluate phenol biodregadation capacity in Guarango(Caesalpina spinosa) tannic extract phenols and in indigo carmine, orange II and red phenols, common residues in the textile industry. Two types of fungi were used, white-rot Trametes Versicolor fungus and the filamentous Aspergillus niger. Biological treatments were applied in triplicate trough the spectrophotometric UV method for 15 days. When applying the Trametes Versicolor fungis to the tannic extract, there was 69.45% degradation, and a 100% discoloration in the indigo carmine. When applying Aspergillus niger fungus to the tannic extract and orange II dye, there was a 63.45% and 32.78% degradatión, respectively. There was a discoloration no greater than 26% when applying Trametes Versicolor and Aspergillus niger to the red phenol. The fungi applied during the biological treatement presented tolerance to both the tannic extract composition as well as the concentration of each dye used, verified through growing kinetics of each fungu
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GUARANGO EXTRACT (Caesalpinia Spinosa) AND INDIGO CARMINE, ORANGE II, AND RED DYES PHENOL BIODEGRADATION THROUGH Trametes Versicolor AND Aspergillus Niger
'Salesian Polytechnic University of Ecuador', 2014Co-Authors: Huachi Laura, Macas Ángela, Méndez GabrielaAbstract:Esta investigación se la realizó con el fin de evaluar la capacidad de biodegradación de los fenoles presentes en el extracto de tánico de guarango (Caesalpinia spinosa) y en los colorantes índigo carmín, naranja II y rojo fenol, presentes en los desechos de la industria textil. Se utilizaron dos tipos de hongos, el de podredumbre blanca Trametes Versicolor y filamentoso Aspergillus niger. Los tratamientos biológicos se aplicaron por triplicado, realizados por el método espectrofotométrico UV por 15 días; los resultados obtenidos al aplicar el hongo Trametes Versicolor en el extracto tánico fue del 69,45 % de degradación; y en el colorante índigo carmín fue del 100 % de decoloración. Los resultados de la degradación con el hongo Aspergillus niger sobre el extracto tánico fue del 63,45 % y en el colorante naranja II fue del 32,78 %. En la aplicación de Trametes Versicolor y Aspergillus niger sobre el colorante rojo fenol se obtuvieron porcentajes de decoloración no mayores al 26 %. Los hongos aplicados en los tratamientos biológicos presentaron tolerancia tanto a la composición del extracto tánico como a las concentraciones de cada uno de los colorantes usados, esto fue comprobado con la cinética de crecimiento para cada hongo.This research was done to evaluate phenol biodegradation capacity in Guarango (Caesalpinia spinosa) tannic extract phenols and in indigo carmine, orange II and red phenols, common residues in the textile industry. Two types of fungi were used, white-rot Trametes Versicolor fungus and the filamentous Aspergillus niger. Biological treatments were applied in triplicate through the spectrophotometric UV method for 15 days. When applying the Trametes Versicolor fungus to the tannic extract, there was 69.45 % degradation, and a 100 % discoloration in the indigo carmine. When applying the Aspergillus niger fungus to the tannic extract and orange II dye, there was a 63,45 % and 32,78 % degradation, respectively. There was a discoloration no greater than 26 % when applying Trametes Versicolor and Aspergillus niger to the red phenol. The fungi applied during the biological treatment presented tolerance to both the tannic extract composition as well as the concentration of each dye used, verified through growing kinetics of each fungus
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Biodegradación de los fenoles presentes en el extracto de guarango (caesalpinia spinosa) y en los colorantes índigo carmín, naranja ii y rojo fenol a través de Trametes Versicolor y aspergillus niger
2014Co-Authors: Huachi Laura, Macías Ángel, Méndez GabrielaAbstract:This research was done to evaluate phenol biodegradation capacity in Guarango (Caesalpinia spinosa) tannic extract phenols and in indigo carmine, orange II and red phenols, common residues in the textile industry. Two types of fungi were used, white-rot Trametes Versicolor fungus and the filamentous Aspergillus niger. Biological treatments were applied in triplicate through the spectrophotometric UV method for 15 days. When applying the Trametes Versicolor fungus to the tannic extract, there was 69.45 % degradation, and a 100 % discoloration in the indigo carmine. When applying the Aspergillus niger fungus to the tannic extract and orange II dye, there was a 63,45 % and 32,78 % degradation, respectively. There was a discoloration no greater than 26 % when applying Trametes Versicolor and Aspergillus niger to the red phenol. The fungi applied during the biological treatment presented tolerance to both the tannic extract composition as well as the concentration of each dye used, verified through growing kinetics of each fungus.Esta investigación se la realizó con el fin de evaluar la capacidad de biodegradación de los fenoles presentes en el extracto de tánico de guarango (Caesalpinia spinosa) y en los colorantes índigo carmín, naranja II y rojo fenol, presentes en los desechos de la industria textil. Se utilizaron dos tipos de hongos, el de podredumbre blanca Trametes Versicolor y filamentoso Aspergillus niger. Los tratamientos biológicos se aplicaron por triplicado, realizados por el método espectrofotométrico UV por 15 días; los resultados obtenidos al aplicar el hongo Trametes Versicolor en el extracto tánico fue del 69,45 % de degradación; y en el colorante índigo carmín fue del 100 % de decoloración. Los resultados de la degradación con el hongo Aspergillus niger sobre el extracto tánico fue del 63,45 % y en el colorante naranja II fue del 32,78 %. En la aplicación de Trametes Versicolor y Aspergillus niger sobre el colorante rojo fenol se obtuvieron porcentajes de decoloración no mayores al 26 %. Los hongos aplicados en los tratamientos biológicos presentaron tolerancia tanto a la composición del extracto tánico como a las concentraciones de cada uno de los colorantes usados, esto fue comprobado con la cinética de crecimiento para cada hongo
Gloria Caminal - One of the best experts on this subject based on the ideXlab platform.
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degradation of pharmaceuticals from membrane biological reactor sludge with Trametes Versicolor
Environmental Science: Processes & Impacts, 2015Co-Authors: Guillem Llorensblanch, Gloria Caminal, Marina Badiafabregat, Daniel Lucas, Sara Rodriguezmozaz, Damia Barcelo, Taina Pennanen, Paqui BlanquezAbstract:Emerging contaminants are a wide group of chemical products that are found at low concentrations in the environment. These contaminants can be either natural, e.g., estrogens, or synthetics, such as pesticides and pharmaceuticals, which can enter the environment through the water and sludge from wastewater treatment plants (WWTP). The growth of Trametes Versicolor on membrane biological reactor (MBR) sludge in bioslurry systems at the Erlenmeyer scale was assessed and its capacity for removing pharmaceutical and personal care products (PPCPs) was evaluated. The ability of the fungus to remove hydrochlorothiazide (HZT) from liquid media cultures was initially assessed. Consequently, different bioslurry media (complete nutrient, glucose and no-nutrient addition) and conditions (sterile and non-sterile) were tested, and the removal of spiked HZT was monitored under each condition. The highest spiked HZT removal was assessed under non-sterile conditions without nutrient addition (93.2%). Finally, the removal assessment of a broad set of pharmaceuticals was performed in non-spiked bioslurry. Under non-sterile conditions, the fungus was able to completely degrade 12 out of the 28 drugs initially detected in the MBR sludge, achieving an overall degradation of 66.9%. Subsequent microbial analysis showed that the microbial diversity increased after 15 days of treatment, but there was still some T. Versicolor in the bioslurry. Results showed that T. Versicolor can be used to remove PPCPs in bioslurry systems under non-sterile conditions, without extra nutrients in the media, and in matrices as complex as an MBR sludge.
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soil colonization by Trametes Versicolor grown on lignocellulosic materials substrate selection and naproxen degradation
International Biodeterioration & Biodegradation, 2011Co-Authors: Eduard Borras, Carlos E Rodriguezrodriguez, Montserrat Sarrà, Guillem Llorensblanch, Gloria CaminalAbstract:Abstract The ability of Trametes Versicolor to degrade soil pollutants has been widely studied. However, the use of such fungus in real soil applications has lead to dissimilar results mainly due to soil colonization limitations. Therefore, it is important to investigate techniques to improve the survival of this white rot fungus in soils. In the present study, several processed and unprocessed low-cost lignocellulosic substrates were employed as inoculum carriers for fungal growth prior to application in soil for bioaugmentation. The fungal growth was determined by means of laccase activity and ergosterol determinations; additionally, the degrading capacity was measured by the naproxen degradation test (ND24). Although T. Versicolor was able to colonize all materials, the colonization and enzymatic production was higher on processed agricultural wastes with relative low C/N ratios than in raw lignocellulosic substrates. Soil colonization was successful under both sterile and non-sterile conditions when amended with processed agricultural wastes, yielding even higher laccase production in non-sterile conditions. Moreover, T. Versicolor was able to degrade significant amounts of spiked naproxen after 24 h in sterile and non-sterile soil cultures, showing the best results when using a material based on wheat straw as carrier.
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solid phase treatment with the fungus Trametes Versicolor substantially reduces pharmaceutical concentrations and toxicity from sewage sludge
Bioresource Technology, 2011Co-Authors: Carlos E Rodriguezrodriguez, Gloria Caminal, Aleksandra Jelic, Marta Llorca, Marinella Farre, Mira Petrovic, Teresa VicentAbstract:For safe biosolid-land-applying, sludge should be contaminant-free. However, it may contain important amounts of micropollutants, not removed in the wastewater-treatment-processes. An alternative treatment with the fungus Trametes Versicolor was applied in sterile solid-phase systems consisting of sludge and a lignocellulosic substrate. Fungal colonization and activity were demonstrated during the process, according to monitoring of ergosterol, laccase activity and the naproxen-degradation test (ND24). Fourteen out of 43 analyzed pharmaceuticals were found in the raw sludge. After treatment, phenazone, bezafibrate, fenofibrate, cimetidine, clarithromycin, sulfamethazine and atenolol were completely removed, while removals between 42% and 80% were obtained for the remaining pharmaceuticals. Toxicological analyses (Daphnia magna, Vibrio fischeri and seed germination) showed an important reduction in sludge toxicity after treatment. Results suggest that a solid-phase treatment with T. Versicolor may reduce the ecotoxicological impact of micropollutants present in sewage sludge. This is the first report of a fungal-approach for elimination of emerging pollutants from biosolids.
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Metabolites from the biodegradation of triphenylmethane dyes by Trametes Versicolor or laccase
Chemosphere, 2009Co-Authors: N. Casas, Teodor Parella, Teresa Vicent, Gloria Caminal, Montserrat SarràAbstract:The feasibility of degrading triphenylmethane dyes by Trametes Versicolor and laccase has been investigated for the following dyes: Acid Fuchsin, Brilliant Green 1, Basic Fuchsin, Methyl Green or Acid Green 16. The toxicity level of triphenylmethane dyes is linked to their basic character, but significant detoxification is obtained when there is biodegradation. Identification of enzymatic degradation products by (1)H NMR made it possible to propose a general rule for the laccase attack on triphenylmethane compounds. The enzyme completely degrades the molecular part of the canonical resonance substructures of dyes, because no N-substituted, mono-N and di-N,N substituted p-amine aromatic residues seem to be wholly degraded. No enzymatic degradation is observed in the cases of either the non-substituted or trisubstituted-N,N,Np-amine aromatic residues. On the other hand, for all the dyes tested, no aromatic residues are detected after fungal treatment; this means that T. Versicolor is more capable of performing further degradation than is laccase. The results of this study demonstrated that compounds with a triphenylmethane structure can be degraded by T. Versicolor even if they are highly toxic. The enzyme laccase plays an important role in the attack on the structure and a general rule for predicting which products would be obtained after the enzymatic treatment is suggested.
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mechanistics of trichloroethylene mineralization by the white rot fungus Trametes Versicolor
Chemosphere, 2008Co-Authors: Ernest Marcourrea, Teodor Parella, Teresa Vicent, Gloria Caminal, Xavier Gabarrell, Adinarayana C ReddyAbstract:Abstract The white-rot fungus Trametes Versicolor degraded trichloroethylene (TCE), a highly oxidized chloroethene, and produced 2,2,2-trichloroethanol and carbon dioxide as the main products of degradation, based on the results obtained using [13C]-TCE as the substrate. For a range of concentrations of TCE between 2 and 20 mg l−1, 53% of the theoretical maximum chloride expected from complete degradation of TCE was observed. Laccase was shown to be induced by TCE, but did not appear to play a role in TCE degradation. Cytochrome P-450 appears to be involved in TCE degradation, as evidenced by marked inhibition of degradation of TCE in the presence of 1-aminobenzotriazole, a known inhibitor of cytochrome P-450. Our results suggested that chloral (trichloroacetaldehyde) was an intermediate of the TCE degradation pathway. The results indicate that the TCE degradation pathway in T. Versicolor appears to be similar to that previously reported in mammals and is mechanistically quite different from bacterial TCE degradation.
Huachi Laura - One of the best experts on this subject based on the ideXlab platform.
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BIODEGRADACIÓN DE LOS FENOLES PRESENTES EN EL EXTRACTO DE GUARANGO(Caesalpinia Spinosa) Y EN LOS COLIRANTES ÍNDIGO CARMÍN, NARANJA II Y ROJO FENOL A TRAVÉZ DE Trametes Versicolor AND Aspergillus Niger.
2014Co-Authors: Huachi Laura, Macas, Ángela Macas, Méndez GabrielaAbstract:Esta investigación se la realizó con el fin de evaluar la capacidad de biodegradación de los fenoles presentes en el extracto de tánico de guarango((Caesalpina spinosa) y en los colorantes índigo carmín, naranja II y el rojo fenol, presentes en los desechos de la industria textil. Se utilizaron dos tipos de hongos, el de podredumbr blanca Trametes Versicolor y filamentos Aspergillus niger : Los tratamientos bológicos se aplicaron por triplicado, reaoizados por el método expectrofotométrico Uv por 15 días; los resultados obtenidos al aplicar el hongo Trametes Versicolor en el extracto titáico fue del 69.45% de degradación; y en el colorante índigo carmín fue del 100% de decoloración. Los resultados de la degradación de Trametes Versicolor y Aspergillus niger sobre el colorante rojo fenol se obtuvieron porcentajes de decoloración no mayores al 26%. Los hongos aplicados en los tratamientos biológicos presentaron tolerancia tanto a la composición del extracto tánico como a las concentraciones de cada uno de los colorantes usados, esto fue comprobado con la cinética de crecimiento para cada hongo.The research was done to evaluate phenol biodregadation capacity in Guarango(Caesalpina spinosa) tannic extract phenols and in indigo carmine, orange II and red phenols, common residues in the textile industry. Two types of fungi were used, white-rot Trametes Versicolor fungus and the filamentous Aspergillus niger. Biological treatments were applied in triplicate trough the spectrophotometric UV method for 15 days. When applying the Trametes Versicolor fungis to the tannic extract, there was 69.45% degradation, and a 100% discoloration in the indigo carmine. When applying Aspergillus niger fungus to the tannic extract and orange II dye, there was a 63.45% and 32.78% degradatión, respectively. There was a discoloration no greater than 26% when applying Trametes Versicolor and Aspergillus niger to the red phenol. The fungi applied during the biological treatement presented tolerance to both the tannic extract composition as well as the concentration of each dye used, verified through growing kinetics of each fungu
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GUARANGO EXTRACT (Caesalpinia Spinosa) AND INDIGO CARMINE, ORANGE II, AND RED DYES PHENOL BIODEGRADATION THROUGH Trametes Versicolor AND Aspergillus Niger
'Salesian Polytechnic University of Ecuador', 2014Co-Authors: Huachi Laura, Macas Ángela, Méndez GabrielaAbstract:Esta investigación se la realizó con el fin de evaluar la capacidad de biodegradación de los fenoles presentes en el extracto de tánico de guarango (Caesalpinia spinosa) y en los colorantes índigo carmín, naranja II y rojo fenol, presentes en los desechos de la industria textil. Se utilizaron dos tipos de hongos, el de podredumbre blanca Trametes Versicolor y filamentoso Aspergillus niger. Los tratamientos biológicos se aplicaron por triplicado, realizados por el método espectrofotométrico UV por 15 días; los resultados obtenidos al aplicar el hongo Trametes Versicolor en el extracto tánico fue del 69,45 % de degradación; y en el colorante índigo carmín fue del 100 % de decoloración. Los resultados de la degradación con el hongo Aspergillus niger sobre el extracto tánico fue del 63,45 % y en el colorante naranja II fue del 32,78 %. En la aplicación de Trametes Versicolor y Aspergillus niger sobre el colorante rojo fenol se obtuvieron porcentajes de decoloración no mayores al 26 %. Los hongos aplicados en los tratamientos biológicos presentaron tolerancia tanto a la composición del extracto tánico como a las concentraciones de cada uno de los colorantes usados, esto fue comprobado con la cinética de crecimiento para cada hongo.This research was done to evaluate phenol biodegradation capacity in Guarango (Caesalpinia spinosa) tannic extract phenols and in indigo carmine, orange II and red phenols, common residues in the textile industry. Two types of fungi were used, white-rot Trametes Versicolor fungus and the filamentous Aspergillus niger. Biological treatments were applied in triplicate through the spectrophotometric UV method for 15 days. When applying the Trametes Versicolor fungus to the tannic extract, there was 69.45 % degradation, and a 100 % discoloration in the indigo carmine. When applying the Aspergillus niger fungus to the tannic extract and orange II dye, there was a 63,45 % and 32,78 % degradation, respectively. There was a discoloration no greater than 26 % when applying Trametes Versicolor and Aspergillus niger to the red phenol. The fungi applied during the biological treatment presented tolerance to both the tannic extract composition as well as the concentration of each dye used, verified through growing kinetics of each fungus
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Biodegradación de los fenoles presentes en el extracto de guarango (caesalpinia spinosa) y en los colorantes índigo carmín, naranja ii y rojo fenol a través de Trametes Versicolor y aspergillus niger
2014Co-Authors: Huachi Laura, Macías Ángel, Méndez GabrielaAbstract:This research was done to evaluate phenol biodegradation capacity in Guarango (Caesalpinia spinosa) tannic extract phenols and in indigo carmine, orange II and red phenols, common residues in the textile industry. Two types of fungi were used, white-rot Trametes Versicolor fungus and the filamentous Aspergillus niger. Biological treatments were applied in triplicate through the spectrophotometric UV method for 15 days. When applying the Trametes Versicolor fungus to the tannic extract, there was 69.45 % degradation, and a 100 % discoloration in the indigo carmine. When applying the Aspergillus niger fungus to the tannic extract and orange II dye, there was a 63,45 % and 32,78 % degradation, respectively. There was a discoloration no greater than 26 % when applying Trametes Versicolor and Aspergillus niger to the red phenol. The fungi applied during the biological treatment presented tolerance to both the tannic extract composition as well as the concentration of each dye used, verified through growing kinetics of each fungus.Esta investigación se la realizó con el fin de evaluar la capacidad de biodegradación de los fenoles presentes en el extracto de tánico de guarango (Caesalpinia spinosa) y en los colorantes índigo carmín, naranja II y rojo fenol, presentes en los desechos de la industria textil. Se utilizaron dos tipos de hongos, el de podredumbre blanca Trametes Versicolor y filamentoso Aspergillus niger. Los tratamientos biológicos se aplicaron por triplicado, realizados por el método espectrofotométrico UV por 15 días; los resultados obtenidos al aplicar el hongo Trametes Versicolor en el extracto tánico fue del 69,45 % de degradación; y en el colorante índigo carmín fue del 100 % de decoloración. Los resultados de la degradación con el hongo Aspergillus niger sobre el extracto tánico fue del 63,45 % y en el colorante naranja II fue del 32,78 %. En la aplicación de Trametes Versicolor y Aspergillus niger sobre el colorante rojo fenol se obtuvieron porcentajes de decoloración no mayores al 26 %. Los hongos aplicados en los tratamientos biológicos presentaron tolerancia tanto a la composición del extracto tánico como a las concentraciones de cada uno de los colorantes usados, esto fue comprobado con la cinética de crecimiento para cada hongo
Yakup M Arica - One of the best experts on this subject based on the ideXlab platform.
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biosorption of heavy metal ions on immobilized white rot fungus Trametes Versicolor
Journal of Hazardous Materials, 2003Co-Authors: Gulay Bayramoglu, Sema Bektas, Yakup M AricaAbstract:Abstract Trametes Versicolor mycelia were immobilized in carboxymethylcellulose, CMC, beads via entrapment, and the bead containing immobilized fungus spores were incubated at 30 °C for 3 days to attain uniform growth on the bead surface. After incubation, the live and heat inactivated immobilized fungus on the CMC beads were used for the biosorption of Cu 2+ , Pb 2+ and Zn 2+ ions. Plain CMC beads were used as a control system. The biosorption of Cu 2+ , Pb 2+ and Zn 2+ ions by the CMC and both live and inactivated immobilized preparations increased as the initial concentration of Cu 2+ , Pb 2+ and Zn 2+ ions in the medium increased. The maximum biosorption capacities for both immobilized live and heat inactivated Trametes Versicolor were 1.51 and 1.84 mmol Cu 2+ , 0.85 and 1.11 mmol Pb 2+ and 1.33 and 1.67 mmol Zn 2+ per g of dry biosorbents, respectively. Biosorption equilibrium was established in about 1.0 h and the equilibrium was well described by Langmuir and Freundlich isotherms. A temperature change in the range of 15–45 °C did not affect the biosorption capacity. The affect of pH was also investigated and the maximum adsorption of Cu 2+ , Pb 2+ and Zn 2+ ions on the CMC and both live and inactivated immobilized fungal biomass was observed between pH 4.0 and 6.0. The CMC beads with the immobilized fungus can be regenerated using 10 mM HCl, with up to 97% recovery of the metal ions; the biosorbents reused up to five biosorption–desorption cycles without any major loss in the biosorption capacity.
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biosorption of heavy metal ions on immobilized white rot fungus Trametes Versicolor
Journal of Hazardous Materials, 2003Co-Authors: Gulay Bayramoglu, Sema Bektas, Yakup M AricaAbstract:Trametes Versicolor mycelia were immobilized in carboxymethylcellulose, CMC, beads via entrapment, and the bead containing immobilized fungus spores were incubated at 30 degrees C for 3 days to attain uniform growth on the bead surface. After incubation, the live and heat inactivated immobilized fungus on the CMC beads were used for the biosorption of Cu(2+), Pb(2+) and Zn(2+) ions. Plain CMC beads were used as a control system. The biosorption of Cu(2+), Pb(2+) and Zn(2+) ions by the CMC and both live and inactivated immobilized preparations increased as the initial concentration of Cu(2+), Pb(2+) and Zn(2+) ions in the medium increased. The maximum biosorption capacities for both immobilized live and heat inactivated Trametes Versicolor were 1.51 and 1.84mmol Cu(2+), 0.85 and 1.11mmol Pb(2+) and 1.33 and 1.67mmol Zn(2+) per g of dry biosorbents, respectively. Biosorption equilibrium was established in about 1.0h and the equilibrium was well described by Langmuir and Freundlich isotherms. A temperature change in the range of 15-45 degrees C did not affect the biosorption capacity. The affect of pH was also investigated and the maximum adsorption of Cu(2+), Pb(2+) and Zn(2+) ions on the CMC and both live and inactivated immobilized fungal biomass was observed between pH 4.0 and 6.0. The CMC beads with the immobilized fungus can be regenerated using 10mM HCl, with up to 97% recovery of the metal ions; the biosorbents reused up to five biosorption-desorption cycles without any major loss in the biosorption capacity.
Teresa Vicent - One of the best experts on this subject based on the ideXlab platform.
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phenol and color removal in hydrous ethanol vinasse in an air pulsed bioreactor using Trametes Versicolor
Journal of Biochemical Technology, 2015Co-Authors: Elda Espanagamboa, Teresa Vicent, Xavier Font, Javier O Mijangoscortes, Blondy Cantocanche, Liliana AlzateAbstract:Vinasse is a problem in worldwide and one that is predicted to increase over the years to come. Total phenol and color removal from this type of vinasse was evaluated using an air-pulsed bioreactor inoculated with Trametes Versicolor . Batch operation of the bioreactor removed 71% of total phenol, 18% of color and 40% of chemical oxygen demand (COD). Maximum laccase activity achieved was 428 U/L. The air-pulsed bioreactor was subsequently operated in continuous mode for a period of 25 days, removing 80% of total phenol, 17% of color and 60% of COD. Laccase activity ranged from 956 to 1630 U/L. The results indicate that continuous operation of an air-pulsed bioreactor under the conditions proposed in this study favored biodegradation of vinasse.
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solid phase treatment with the fungus Trametes Versicolor substantially reduces pharmaceutical concentrations and toxicity from sewage sludge
Bioresource Technology, 2011Co-Authors: Carlos E Rodriguezrodriguez, Gloria Caminal, Aleksandra Jelic, Marta Llorca, Marinella Farre, Mira Petrovic, Teresa VicentAbstract:For safe biosolid-land-applying, sludge should be contaminant-free. However, it may contain important amounts of micropollutants, not removed in the wastewater-treatment-processes. An alternative treatment with the fungus Trametes Versicolor was applied in sterile solid-phase systems consisting of sludge and a lignocellulosic substrate. Fungal colonization and activity were demonstrated during the process, according to monitoring of ergosterol, laccase activity and the naproxen-degradation test (ND24). Fourteen out of 43 analyzed pharmaceuticals were found in the raw sludge. After treatment, phenazone, bezafibrate, fenofibrate, cimetidine, clarithromycin, sulfamethazine and atenolol were completely removed, while removals between 42% and 80% were obtained for the remaining pharmaceuticals. Toxicological analyses (Daphnia magna, Vibrio fischeri and seed germination) showed an important reduction in sludge toxicity after treatment. Results suggest that a solid-phase treatment with T. Versicolor may reduce the ecotoxicological impact of micropollutants present in sewage sludge. This is the first report of a fungal-approach for elimination of emerging pollutants from biosolids.
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Metabolites from the biodegradation of triphenylmethane dyes by Trametes Versicolor or laccase
Chemosphere, 2009Co-Authors: N. Casas, Teodor Parella, Teresa Vicent, Gloria Caminal, Montserrat SarràAbstract:The feasibility of degrading triphenylmethane dyes by Trametes Versicolor and laccase has been investigated for the following dyes: Acid Fuchsin, Brilliant Green 1, Basic Fuchsin, Methyl Green or Acid Green 16. The toxicity level of triphenylmethane dyes is linked to their basic character, but significant detoxification is obtained when there is biodegradation. Identification of enzymatic degradation products by (1)H NMR made it possible to propose a general rule for the laccase attack on triphenylmethane compounds. The enzyme completely degrades the molecular part of the canonical resonance substructures of dyes, because no N-substituted, mono-N and di-N,N substituted p-amine aromatic residues seem to be wholly degraded. No enzymatic degradation is observed in the cases of either the non-substituted or trisubstituted-N,N,Np-amine aromatic residues. On the other hand, for all the dyes tested, no aromatic residues are detected after fungal treatment; this means that T. Versicolor is more capable of performing further degradation than is laccase. The results of this study demonstrated that compounds with a triphenylmethane structure can be degraded by T. Versicolor even if they are highly toxic. The enzyme laccase plays an important role in the attack on the structure and a general rule for predicting which products would be obtained after the enzymatic treatment is suggested.
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mechanistics of trichloroethylene mineralization by the white rot fungus Trametes Versicolor
Chemosphere, 2008Co-Authors: Ernest Marcourrea, Teodor Parella, Teresa Vicent, Gloria Caminal, Xavier Gabarrell, Adinarayana C ReddyAbstract:Abstract The white-rot fungus Trametes Versicolor degraded trichloroethylene (TCE), a highly oxidized chloroethene, and produced 2,2,2-trichloroethanol and carbon dioxide as the main products of degradation, based on the results obtained using [13C]-TCE as the substrate. For a range of concentrations of TCE between 2 and 20 mg l−1, 53% of the theoretical maximum chloride expected from complete degradation of TCE was observed. Laccase was shown to be induced by TCE, but did not appear to play a role in TCE degradation. Cytochrome P-450 appears to be involved in TCE degradation, as evidenced by marked inhibition of degradation of TCE in the presence of 1-aminobenzotriazole, a known inhibitor of cytochrome P-450. Our results suggested that chloral (trichloroacetaldehyde) was an intermediate of the TCE degradation pathway. The results indicate that the TCE degradation pathway in T. Versicolor appears to be similar to that previously reported in mammals and is mechanistically quite different from bacterial TCE degradation.
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novel aerobic perchloroethylene degradation by the white rot fungus Trametes Versicolor
Environmental Science & Technology, 2006Co-Authors: Ernest Marcourrea, Teresa Vicent, Gloria Caminal, Xavier Gabarrell, Montserrat Sarrà, Adinarayana C ReddyAbstract:Perchloroethylene (PCE) is one of the most important groundwater pollutants around the world. It is a suspected carcinogen and is believed to be recalcitrant to microbial degradation. We report here, for the first time, aerobic degradation of PCE by the white rot fungus, Trametes Versicolor, to less hazardous products. Aerobic degradation rate of PCE was 0.20 and 0.28 nmol h(-1) mg(-1) dry weight of fungal biomass. Trichloroacetic acid (TCA) was identified as the main intermediate using [2-13C]-PCE as the substrate. Chloride released and TCA produced were stoichiometric with PCE degradation. Our studies using 1 -aminobenzotriazole (ABT), an inhibitor of cytochrome P-450, suggested that a cytochrome P-450 system may be involved in PCE degradation by T. Versicolor. These results are of particular interest because TCA production from PCE has not been reported to date in bacteria or fungi.