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Yasir Hasan Siddique - One of the best experts on this subject based on the ideXlab platform.
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Effect of luteolin on the Transgenic Drosophila model of Huntington’s disease
Computational Toxicology, 2021Co-Authors: Yasir Hasan Siddique, Rahul, Himanshi Varshney, I. Mantasha, Mohd. ShahidAbstract:Abstract Huntington disease (HD) is a progressive neurodegenerative disorder which deteriorates the physical and mental abilities of the patients. It is an autosomal dominant disorder and is mainly caused by the expansion of a repeating CAG triplet. The abnormal expansion of CAG trinucleotide (polyQ repeats) on the N-terminus of the Htt gene (coding the protein huntingtin, HTT) is considered to be the one of the most important factor for this disease. The mutant form of huntingtin (mHTT) not only leads to the neuronal dysfunction but also increase the oxidative stress in the neurons. In this context plant derivatives could be good therapeutic agents with minimum side effects. Luteolin (3′,4′,5,7-tetrahydroxyflavone) is abundantly present in many fruits and vegetables. It is a well-known anti-oxidant and has been reported to reduce oxidative stress in various models of neurodegenerative diseases. The final doses of luteolin i.e. 25, 50, 75 and 100 µM were established in the diet and the HD flies were allowed to feed on it for 33 days. The exposure of luteolin to HD flies showed not only a dose dependent delay in the loss of climbing ability but also a dose dependent significant decrease in the oxidative stress compared to unexposed HD flies. The molecular docking studies suggest a positive interaction between mHTT and luteolin. The results suggest that the luteolin is effective in reducing the HD symptoms in Transgenic Drosophila model of HD.
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Effect of polyherbal drug Majun falasfa on the Transgenic Drosophila model of Parkinson’s Disease
Current Traditional Medicine, 2020Co-Authors: Yasir Hasan Siddique, Falaq Naz, Mohammad RashidAbstract:Aim: The effect of Majun Falasfa (MF) was studied on the Transgenic Drosophila expressing human alpha synuclein panneurally. Background: MF is a Unani medicine used for enhancing mental power and treating kidney, joint pains and urinary tract diseases. It is also use for phlegmatic diseases. It is also being used in age related dementia and to counter the effects of ageing. Methods: The equivalents of recommended dose for human were established for 20g of fly food i.e. 0.0014, 0.0028, 0.0042 and 0.0056g per 20g of diet. The PD flies were allowed to feed on it for 24 days before studying its effect on cognitive and oxidative stress parameters. Immunohistochemistry was also performed study the effect of MF on human alpha synuclein expression. Results: The exposure to MF increased the life span and improves the activity of PD flies. MF delayed the loss of climbing ability of PD flies. The exposure of PD flies to MF significantly reduced the oxidative stress and improves the antioxidant enzymes homeostasis compared to unexposed PD flies. The exposure to MF reduces the formation of Lewy bodies as is evident by immunohistochemistry. Conclusion: MF is potent in reducing the PD (Parkinson’s disease) symptoms being mimicked in the Transgenic flies.
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Effect of Cabergoline on Cognitive Impairments in Transgenic Drosophila Model of Parkinson’s Disease
Letters in Drug Design & Discovery, 2020Co-Authors: Yasir Hasan Siddique, Rahul, Mantasha Idrisi, Mohd. ShahidAbstract:Background: Parkinson’s disease is a common neurodegenerative disorder characterized by selective loss of dopaminergic neurons in the substantia nigra pars compacta. Introduction: The effects of alpha synuclein, parkin mutation and pharmacological agents have been studied in the Drosophila model. Methods: The effect of cabergoline was studied on the cognitive impairments exhibited by the Transgenic Drosophila expressing human alpha-synuclein in the neurons. The PD flies were allowed to feed on the diet having 0.5, 1 and 1.5 μM of cabergoline. Results and Discussion: The exposure of cabergoline not only showed a dose-dependent significant delay in the cognitive impairments but also prevented the loss of dopaminergic neurons. Molecular docking studies showed the positive interaction between cabergoline and alpha-synuclein. Conclusion: The results suggest a protective effect of cabergoline against the cognitive impairments.
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Effect of kaempferol on the Transgenic Drosophila model of Parkinson's disease.
Scientific reports, 2020Co-Authors: Rahul, Smita Jyoti, Falaq Naz, Yasir Hasan SiddiqueAbstract:The present study was aimed to study the effect of kaempferol, on the Transgenic Drosophila model of Parkinson’s disease. Kaempferol was added in the diet at final concentration of 10, 20, 30 and 40 µM and the effect was studied on various cognitive and oxidative stress markers. The results of the study showed that kaempferol, delayed the loss of climbing ability as well as the activity of PD flies in a dose dependent manner compared to unexposed PD flies. A dose-dependent reduction in oxidative stress markers was also observed. Histopathological examination of fly brains using anti-tyrosine hydroxylase immunostaining has revealed a significant dose-dependent increase in the expression of tyrosine hydroxylase in PD flies exposed to kaempferol. Molecular docking results revealed that kaempferol binds to human alpha synuclein at specific sites that might results in the inhibition of alpha synuclein aggregation and prevents the formation of Lewy bodies.
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Effect of geraniol against arecoline induced toxicity in the third instar larvae of Transgenic Drosophila melanogaster (hsp70-lacZ) Bg9.
Toxicology mechanisms and methods, 2018Co-Authors: Barkha Shakya, Sonam Shakya, Yasir Hasan SiddiqueAbstract:In the present study geraniol at the final concentration of 10, 20, 30, and 40 µM was mixed in the diet along with 80 µM of arecoline and the third instar larvae of Transgenic Drosophila melanogaster (hsp70-lacZ) Bg9 were allowed to feed on it for 24 hrs. After the exposure of 24 hrs the larvae were subjected to ONPG, X-gal, trypan blue exclusion test, oxidative stress markers and apoptotic and comet assays. The exposure of larvae to geraniol showed a dose dependent decrease in the activity of β-galactosidase, tissue damage and oxidative stress markers. A dose dependent decrease in apoptosis and DNA damage was also observed. Molecular docking studies also support the protective role of geraniol against the arecoline induced toxicity. The results suggest that geraniol is potent in reducing the toxicity induced by arecoline in the third instar larvae of Transgenic Drosophila melanogaster (hsp70-lacZ) Bg9.
Roel Nusse - One of the best experts on this subject based on the ideXlab platform.
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Creating Transgenic Drosophila by microinjecting the site-specific φC31 integrase mRNA and a transgene-containing donor plasmid
Nature Protocols, 2007Co-Authors: Matthew P Fish, Amy C. Groth, Michele P. Calos, Roel NusseAbstract:We describe a microinjection-based φC31 integrase mRNA-mediated method for creating Transgenic Drosophila strains. This approach is more efficient than traditional methods and ensures that the transgene is targeted to a precise genomic position. The method involves targeting integration of an exogenous plasmid (containing the transgene and sequences to facilitate integration) to a preplaced recipient site in the Drosophila genome. The plasmid is coinjected into embryos with mRNA encoding the φC31 integrase, the enzyme that catalyzes the integration reaction. Using the protocol described here, Transgenic lines can be established from, on average, 46% of fertile adults obtained after injection, and all integrations should be targeted to the chosen genomic insertion site. The whole procedure, from injection to established Transgenic stocks, can be completed in three generations (approximately 1 month) and can be adapted for other types of transgenesis and mRNA injections in Drosophila .
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creating Transgenic Drosophila by microinjecting the site specific phi c31 integrase mrna and a transgene containing donor plasmid
Nature Protocols, 2007Co-Authors: Matt Fish, Amy C. Groth, Michele P. Calos, Roel NusseAbstract:Creating Transgenic Drosophila by microinjecting the site-specific φC31 integrase mRNA and a transgene-containing donor plasmid
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Creating Transgenic Drosophila by microinjecting the site-specific |[phi]|C31 integrase mRNA and a transgene-containing donor plasmid
Nature protocols, 2007Co-Authors: Matt Fish, Amy C. Groth, Michele P. Calos, Roel NusseAbstract:Creating Transgenic Drosophila by microinjecting the site-specific φC31 integrase mRNA and a transgene-containing donor plasmid
Smita Jyoti - One of the best experts on this subject based on the ideXlab platform.
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Effect of kaempferol on the Transgenic Drosophila model of Parkinson's disease.
Scientific reports, 2020Co-Authors: Rahul, Smita Jyoti, Falaq Naz, Yasir Hasan SiddiqueAbstract:The present study was aimed to study the effect of kaempferol, on the Transgenic Drosophila model of Parkinson’s disease. Kaempferol was added in the diet at final concentration of 10, 20, 30 and 40 µM and the effect was studied on various cognitive and oxidative stress markers. The results of the study showed that kaempferol, delayed the loss of climbing ability as well as the activity of PD flies in a dose dependent manner compared to unexposed PD flies. A dose-dependent reduction in oxidative stress markers was also observed. Histopathological examination of fly brains using anti-tyrosine hydroxylase immunostaining has revealed a significant dose-dependent increase in the expression of tyrosine hydroxylase in PD flies exposed to kaempferol. Molecular docking results revealed that kaempferol binds to human alpha synuclein at specific sites that might results in the inhibition of alpha synuclein aggregation and prevents the formation of Lewy bodies.
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Protective Effect of Kaempferol on the Transgenic Drosophila Model of Alzheimer's Disease.
CNS & neurological disorders drug targets, 2018Co-Authors: Tanveer Beg, Falaq Naz, Smita Jyoti, Fahad Ali, Rahul, Syed Kashif Ali, Ahmed Mohamed Reyad, Yasir Hasan SiddiqueAbstract:BACKGROUND Alzheimer's disease (AD) is characterized by the accumulation and deposition of β-amyloid peptides leading to a progressive neuronal damage and cell loss. Besides several hypotheses for explaining the neurodegenerative mechanisms, oxidative stress has been considered to be one of them. Till date, there is no cure for AD, but the pathogenesis of the disease could be delayed by the use of natural antioxidants. In this context, we decided to study the effect of kaempferol against the Transgenic Drosophila expressing human amyloid beta-42. METHOD The AD flies were allowed to feed on the diet having 10, 20, 30 and 40µM of kaempferol for 30 days. After 30 days of exposure, the amyloid beta flies were studied for their climbing ability and Aversive Phototaxis Suppression assay. Amyloid beta flies head homogenate was prepared for estimating the oxidative stress markers, Caspase and acetylcholinesterase activity. RESULTS The results of the present study reveal that the exposure of AD flies to kaempferol delayed the loss of climbing ability, memory, reduced the oxidative stress and acetylcholinesterase activity. CONCLUSION Kaempferol could be used as a possible therapeutic agent against the progression of the Alzheimer's disease.
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Effect of pramipexole alginate nanodispersion (PAND) on the Transgenic Drosophila expressing human alpha synuclein in the brain
Journal of Applied Biomedicine, 2018Co-Authors: Yasir Hasan Siddique, Falaq Naz, Smita Jyoti, Wasi Khan, Braj Raj Singh, Alim H. NaqviAbstract:In the present study the effect of pramipexole alginate nanodispersion (PAND) was studied on the Transgenic Drosophila exhibiting the PD symptoms. The PD flies were allowed to feed on the diet having PAND at final concentration of 1, 2 and 3 μM. A dose dependent significant delay in the loss of climbing ability and improvement in the activity was observed in PD flies. A dose dependent significant change in the oxidative stress markers and dopamine content was also observed in the PD flies exposed to various doses PAND. The improvement in the PD symptoms was more in PD flies exposed to PAND compared to PD flies exposed to pramipexole alone.
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Evaluation of the toxic potential of aspartame in third instar larvae of Transgenic Drosophila melanogaster (hsp70-lacZ) Bg9
2017Co-Authors: Yasir Hasan Siddique, Simmi Anjum, Rahul X, Smita JyotiAbstract:Aspartame is commonly used as a sweetener in prepared foods, beverages and in recipes. In the present study the possible toxic effects of aspartame were studied in third instar larvae of Transgenic Drosophila melanogaster (hsp70-lacZ) Bg 9 . The third instar larvae were allowed to feed on the diet having different doses of aspartame. The results obtained in the present study suggest that the exposure of third instar larvae to the diet having 20, 40, 80, 160 and 320 mM of aspartame did not show any significant the toxic effects after 24 hours of the exposure compared to control (p
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Alteration in biochemical parameters in the brain of Transgenic Drosophila melanogaster model of Parkinson's disease exposed to apigenin.
Integrative medicine research, 2017Co-Authors: Yasir Hasan Siddique, Smita JyotiAbstract:Abstract Background Oxidative stress is one of the key components of the pathology of various neurodegenerative disorders. Parkinson's disease (PD) is characterized by the progressive loss of dopaminergic neurons owing to the aggregation of alpha-synuclein (αS) in the brain. A number of polyphenols have been reported to inhibit the αS aggregation resulting in the possible prevention of PD. The involvement of free radicals in mediating the neuronal death in PD has also been implicated. Methods In the present study, the Transgenic flies expressing human αS in the brain were exposed to 10μM, 20μM, 40μM, and 80μM of apigenin established in diet for 24 days. Results The flies showed an increase in life span, glutathione, and dopamine content. The exposure of PD flies to various doses of apigenin also results in the reduction of glutathione- S -transferase activity, lipid peroxidation, monoamine oxidase, caspase-3, and caspase-9 activity in a dose-dependent manner. Conclusion The results of the present study reveal that apigenin is potent in increasing the life span, dopamine content, reduced the oxidative stress as well as apoptosis in Transgenic Drosophila model of PD.
Rajindar S. Sohal - One of the best experts on this subject based on the ideXlab platform.
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enhanced catabolism of mitochondrial superoxide hydrogen peroxide and aging in Transgenic Drosophila
Biochemical Journal, 2005Co-Authors: Anne-cécile V. Bayne, Robin J Mockett, William C. Orr, Rajindar S. SohalAbstract:The generation of superoxide anion radicals (O2•−) and hydrogen peroxide (H2O2) during mitochondrial respiration has been widely postulated to be causally linked to the aging process. The hypothesis that a specific enhancement of mitochondrial O2•−/H2O2 catabolism would delay age-associated physiological changes and extend the lifespan was tested by simultaneous overexpression of MnSOD (manganese superoxide dismutase) and catalase, ectopically targeted to the mitochondrial matrix of Transgenic Drosophila melanogaster. The increased activities of these antioxidative enzymes resulted in a decrease of mitochondrial H2O2 release and enhancement of free methionine content. The MnSOD/mitochondrial catalase Transgenic flies displayed an enhanced resistance to experimental oxidative stress, induced by dietary H2O2 administration or by exposure to 100% ambient oxygen. However, the lifespan of the flies was decreased, by up to 43%, and this effect coincided with (i) an overall decrease in physical fitness, as measured by the speed of walking, and (ii) an agerelated decrease in mitochondrial state 3 (ADP-stimulated) respiration. These findings support the notion that mitochondrial O2•−/H2O2 production at physiological levels is essential for normal biological processes leading to the attainment of a normal lifespan.
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Enhanced catabolism of mitochondrial superoxide/hydrogen peroxide and aging in Transgenic Drosophila
Biochemical Journal, 2005Co-Authors: Anne-cécile V. Bayne, Robin J Mockett, William C. Orr, Rajindar S. SohalAbstract:The generation of superoxide anion radicals (O2•−) and hydrogen peroxide (H2O2) during mitochondrial respiration has been widely postulated to be causally linked to the aging process. The hypothesis that a specific enhancement of mitochondrial O2•−/H2O2 catabolism would delay age-associated physiological changes and extend the lifespan was tested by simultaneous overexpression of MnSOD (manganese superoxide dismutase) and catalase, ectopically targeted to the mitochondrial matrix of Transgenic Drosophila melanogaster. The increased activities of these antioxidative enzymes resulted in a decrease of mitochondrial H2O2 release and enhancement of free methionine content. The MnSOD/mitochondrial catalase Transgenic flies displayed an enhanced resistance to experimental oxidative stress, induced by dietary H2O2 administration or by exposure to 100% ambient oxygen. However, the lifespan of the flies was decreased, by up to 43%, and this effect coincided with (i) an overall decrease in physical fitness, as measured by the speed of walking, and (ii) an agerelated decrease in mitochondrial state 3 (ADP-stimulated) respiration. These findings support the notion that mitochondrial O2•−/H2O2 production at physiological levels is essential for normal biological processes leading to the attainment of a normal lifespan.
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Decreased mitochondrial hydrogen peroxide release in Transgenic Drosophila melanogaster expressing intramitochondrial catalase.
Archives of biochemistry and biophysics, 2000Co-Authors: Linda K. Kwong, Robin J Mockett, Anne-cécile V. Bayne, William C. Orr, Rajindar S. SohalAbstract:The objective of this study was to develop strategies for manipulating oxidative stress Transgenically in a multicellular organism. Ectopic catalase was introduced into the mitochondrial matrix, which is the main intracellular site of H2O2 formation and where catalase is normally absent. Transgenic Drosophila melanogaster were generated by microinjection of a P element construct, containing the genomic catalase sequence of Drosophila, with the mitochondrial leader sequence of ornithine aminotransferase inserted upstream of the coding region. Total catalase activities in whole-body homogenates of 10-day-old flies from four Transgenic lines were approximately 30-160% higher than those from the parental and four vector-only control lines. Expression of catalase in the mitochondrial matrix was confirmed by immunoblotting and catalase activity assays. Mitochondrial release of H2O2 was decreased by approximately 90% in the Transgenic lines when compared to levels in vector-only controls. This in vivo system provides a novel model for examining the functional significance of decreased mitochondrial H2O2 release.
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overexpression of glutathione reductase extends survival in Transgenic Drosophila melanogaster under hyperoxia but not normoxia
The FASEB Journal, 1999Co-Authors: Robin J Mockett, Rajindar S. SohalAbstract:The purpose of this study was to test the hypothesis that overexpression of glutathione reductase in Transgenic Drosophila melanogaster increases resistance to oxidative stress and retards the aging process. Transgenic flies were generated by microinjection and subsequent mobilization of a P element construct containing the genomic glutathione reductase gene of Drosophila, with 4 kb upstream and 1.5 kb downstream of the coding region. Transgenic animals stably overexpressed glutathione reductase by up to 100% throughout adult life and under continuous exposure to 100% oxygen or air. Under hyperoxic conditions, overexpressors had increased longevity, decreased accrual of protein carbonyls, and dramatically increased survival rates after recovery from a semi-lethal dose of 100% oxygen. Under normoxic conditions, overexpression of glutathione reductase had no effect on longevity, protein carbonyl content, reduced glutathione, or glutathione disulfide content, although the total consumption of oxygen was slig...
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The effects of catalase gene overexpression on life span and resistance to oxidative stress in Transgenic Drosophila melanogaster.
Archives of biochemistry and biophysics, 1992Co-Authors: William C. Orr, Rajindar S. SohalAbstract:Oxygen free radicals and hydroperoxides have been postulated to play a causal role in the aging process, implying that antioxidant enzymes may act as longevity determinants. Catalase (H2O2:H2O2 oxidoreductase; EC1.11.1.6) is the sole enzyme involved in the elimination of H2O2 in Drosophila melanogaster; glutathione peroxidase being absent. A genomic fragment containing the Drosophila catalase gene was used to construct Transgenic Drosophila lines by means of P element-mediated transformation. Enhanced levels of catalase (up to 80%) did not prolong the life span of flies, nor did they provide improved protection against oxidative stress induced by hyperoxia or paraquat treatment. However, enhanced resistance to hydrogen peroxide was observed in the overexpressors.
Debapratim Kar Chowdhuri - One of the best experts on this subject based on the ideXlab platform.
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Corrigendum to “Adverse effect of organophosphate compounds, dichlorvos and chlorpyrifos in the reproductive tissues of Transgenic Drosophila melanogaster: 70 kDa heat shock protein as a marker of cellular damage” [Toxicology 238 (2007) 1–14]
Toxicology, 2008Co-Authors: Subash C. Gupta, Daya K. Saxena, Neeraj Mathur, Hifzur R. Siddique, Ranjit K. Mishra, Kalyan Mitra, Debapratim Kar ChowdhuriAbstract:Corrigendum to “Adverse effect of organophosphate compounds, dichlorvos and chlorpyrifos in the reproductive tissues of Transgenic Drosophila melanogaster: 70 kDa heat shock protein as a marker of cellular damage” [Toxicology 238 (2007) 1–14] Subash C. Gupta a,1, Hifzur R. Siddique a, Neeraj Mathur b, Ranjit K. Mishra c, Kalyan Mitra d, Daya K. Saxena a, Debapratim K. Chowdhuri a,∗ a Embryotoxicology Section, Industrial Toxicology Research Centre, Lucknow 226001, India b Epidemiology Section, Industrial Toxicology Research Centre, Lucknow 226001, India c Department of Biochemistry, University of Lucknow, Lucknow 226007, India d Electron Microscopy Unit, Central Drug Research Institute, Chattar Manzil, M.G. Marg, Lucknow 226001, Uttar Pradesh, India
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Toxic potential of municipal solid waste leachates in Transgenic Drosophila melanogaster (hsp70-lacZ): hsp70 as a marker of cellular damage.
Ecotoxicology and environmental safety, 2007Co-Authors: Devyani Bhargav, Daya K. Saxena, Mahendra Pratap Singh, Ramesh C. Murthy, Neeraj Mathur, Divya Misra, Debapratim Kar ChowdhuriAbstract:Municipal solid wastes (MSWs) are one of the major sources of environmental pollution. Leachates from these wastes might contaminate the water sources and affect quality of environment. The study was carried out to determine the possible toxic effects of leachates from MSW in Transgenic Drosophila melanogaster (hsp70-lacZ). Third instar larvae exposed to 1.0-3.0% of these leachates at different time intervals were examined for hsp70 expression, oxidative stress enzyme activities, proteotoxicity, tissue damage along with effect on emergence and reproduction. Maximum hsp70 expression was observed in the larvae exposed to highly acidic leachates. Overwhelming of hsp70 expression in the exposed larvae caused a concomitant decline in total protein content and a significant elevation in oxidative stress enzymes and lipid peroxidation (LPO) product. The leachates caused a significant delay in emergence of flies and affected the reproductive performance of the flies at the tested concentrations. The present study highlights the toxic potential of MSW leachates and the advantage of Drosophila as a model to evaluate the impact of leachates at organismal and cellular levels, also advocating Hsp70 as the first tier indicator of toxicity.
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hazardous effects of effluent from the chrome plating industry 70 kda heat shock protein expression as a marker of cellular damage in Transgenic Drosophila melanogaster hsp70 lacz
Environmental Health Perspectives, 2003Co-Authors: Indranil Mukhopadhyay, D K Saxena, Debapratim Kar ChowdhuriAbstract:Hazardous effects of an effluent from the chrome plating industry were examined by exposing Transgenic Drosophila melanogaster (hsp70-lacZ) to various concentrations (0.05, 0.1, 1.0, 10.0, and 100.0 micro L/mL) of the effluent through diet. The emergence pattern of adult flies was affected, along with impaired reproductive performance at the higher dietary concentrations of the effluent. Interestingly, the effect of the effluent was more pronounced in male than in female flies. The effect of the effluent on development of adult flies was concurrent with the expression pattern of the heat shock protein 70 gene (hsp70), both in larval tissues and in the reproductive organs of adult flies. We observed a dose- and time-dependent expression of hsp70 in third instar larvae exposed for different time intervals. Absence of hsp70 expression in larvae exposed to 0.1 micro L/mL of the effluent indicated that this is the highest nontoxic concentration for Drosophila. The stress gene assay in the reproductive organs of adult flies revealed hsp70 expression in the testis of male flies only. However, trypan blue dye exclusion tests in these tissues indicate tissue damage in the male accessory gland of adult flies, which was further confirmed by ultrastructural observations. In the present study we demonstrate the utility of Transgenic Drosophila as an alternative animal model for evaluating hazardous effects of the effluent from the chrome plating industry and further reveal the cytoprotective role of hsp70 and its expression as an early marker in environmental risk assessment.
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Argemone oil induced cellular damage in the reproductive tissues of Transgenic Drosophila melanogaster: protective role of 70 kDa heat shock protein.
Journal of biochemical and molecular toxicology, 2003Co-Authors: Indranil Mukhopadhyay, Daya K. Saxena, Virendra K. Bajpai, Debapratim Kar ChowdhuriAbstract:We explored the reproductive toxicity of argemone oil and its principal alkaloid fraction in Transgenic Drosophila melanogaster (hsp70-lacZ) Bg9. The toxicity of argemone oil has been attributed to two of its physiologically active benzophenanthridine alkaloids, sanguinarine and dihydrosanguinarine. Freshly eclosed first instar larvae of Transgenic Drosophila melanogaster were transferred to different concentrations of argemone oil and its alkaloid fraction contaminated food. Virgin flies that eclosed from the contaminated food were pair-mated to look into the effect on reproduction. The study was further extended by investigating hsp70 expression and tissue damage in larval gonads, genital discs, and reproductive organs of adult fly. Our results showed that argemone oil was more cytotoxic than its principal alkaloid fraction. Moreover, it was the male fly that was more affected compared to its opposite number. The accessory glands of male reproductive system of the fly, which did not express hsp70, exhibited severe damage as evidenced by Trypan blue staining. This prompted us to explore the ultrastructural morphology of the gland, which showed acute signs of necrosis in both the cell types as evident by necrotic nuclei, higher vacuolization, and disorganized endoplasmic reticulum, decrease in the number of Golgi vesicles and disorganized, loosely packed filamentous structures in the lumen of the accessory gland, at the higher concentrations of the adulterant. The study showed the reproductive toxicity of argemone oil and its alkaloid fraction in Transgenic Drosophila melanogaster and further confirmed the cytoprotective role of hsp70. © 2003 Wiley Periodicals, Inc. J Biochem Mol Toxicol 17:223–234, 2003; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.10082.
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Toxicity of argemone oil: Effect on hsp70expression and tissue damage in Transgenic Drosophila melanogaster(hsp70-lacZ) Bg ^9
Cell Biology and Toxicology, 2002Co-Authors: I. Mukhopadhyay, D K Saxena, A. Nazir, K. Mahmood, M. Das, S.k. Khanna, Debapratim Kar ChowdhuriAbstract:The effect of argemone oil on hsp70 expression and tissue damage was investigated by studying β-galactosidase activity, Western blotting and hybridization, and trypan blue staining in the larval tissues of Transgenic Drosophila melanogaster ( hsp70-lacZ ) Bg ^9. Different concentrations of argemone oil were mixed with food and third-instar larvae were allowed to feed on them for different time intervals (2, 4, 24, and 48 h). Argemone oil was found to induce hsp70 even in the lowest concentration of the adulterant while maximum tissue damage was observed in the higher two treatment groups. Malpighian tubules and midgut tissue reflected maximum damage as evidenced by both high β-galactosidase activity and trypan blue staining in these tissues. A prior temperature shock treatment to the larvae was enough to protect the larvae from argemone oil-induced tissue damage as evidenced by little or no trypan blue staining. The present study suggests the cytotoxic potential of argemone oil and further strengthens the evidence for the use of hsp70 as a biomarker in risk assessment.
