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Lidija Senerovic - One of the best experts on this subject based on the ideXlab platform.
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Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts.
Journal of ethnopharmacology, 2018Co-Authors: Ivana Aleksic, Ivana D. Radojević, Petar Ristivojević, Aleksandar Pavic, Ljiljana R. Čomić, Branka Vasiljevic, Dejan Opsenica, Dušanka Milojković-opsenica, Lidija SenerovicAbstract:Abstract Ethnopharmacological relevance Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts. Materials and methods The anti-quorum sensing activity of Tn extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans leafs extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results Subinhibitory concentrations of Tn leaf extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions This study demonstrates anti-virulence potential of Tn leaf extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.
Šenerović Lidija - One of the best experts on this subject based on the ideXlab platform.
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Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts
Elsevier Ireland Ltd Clare, 2018Co-Authors: Aleksić Ivana, Ristivojević Petar, Pavić Aleksandar, Radojević Ivana, Čomić, Ljiljana R., Vasiljević Branka, Opsenica, Dejan M., Milojković-opsenica Dušanka, Šenerović LidijaAbstract:Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts. Materials and methods: The anti-quorum sensing activity of Tn extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans leafs extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn leaf extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn leaf extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.This is the peer-reviewed version of the following article: Aleksic, I.; Ristivojevic, P.; Pavic, A.; Radojević, I.; Čomić, L. R.; Vasiljevic, B.; Opsenica, D.; Milojković-Opsenica, D.; Senerovic, L. Anti-Quorum Sensing Activity, Toxicity in Zebrafish (Danio Rerio) Embryos and Phytochemical Characterization of Trapa natans Leaf Extracts. Journal of Ethnopharmacology 2018, 222, 148–158. [https://doi.org/10.1016/j.jep.2018.05.005]Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/2933
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Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts
'Elsevier BV', 2018Co-Authors: Aleksić Ivana, Ristivojević Petar, Pavić Aleksandar, Radojević Ivana, Čomić, Ljiljana R., Vasiljević Branka, Milojković-opsenica Dušanka, Opsenica Dejan, Šenerović LidijaAbstract:Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts. Materials and methods: The anti-quorum sensing activity of Tn extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans leafs extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn leaf extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn leaf extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.This is the peer-reviewed version of the following article: Aleksic, I.; Ristivojevic, P.; Pavic, A.; Radojević, I.; Čomić, L. R.; Vasiljevic, B.; Opsenica, D.; Milojković-Opsenica, D.; Senerovic, L. Anti-Quorum Sensing Activity, Toxicity in Zebrafish (Danio Rerio) Embryos and Phytochemical Characterization of Trapa natans Leaf Extracts. Journal of Ethnopharmacology 2018, 222, 148–158. [https://doi.org/10.1016/j.jep.2018.05.005][http://cer.ihtm.bg.ac.rs/handle/123456789/2310
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Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts
Elsevier Ireland Ltd Clare, 2018Co-Authors: Aleksić Ivana, Ristivojević Petar, Pavić Aleksandar, Vasiljević Branka, Milojković-opsenica Dušanka, Opsenica Dejan, Radojevic Ivana, Comic, Ljiljana R., Šenerović LidijaAbstract:Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts. Materials and methods: The anti-quorum sensing activity of Tn extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans leafs extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn leaf extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn leaf extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.The peer-reviewed version: [http://cer.ihtm.bg.ac.rs/handle/123456789/3139
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Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts
Elsevier Ireland Ltd Clare, 2018Co-Authors: Aleksić Ivana, Ristivojević Petar, Pavić Aleksandar, Radojević Ivana, Čomić, Ljiljana R., Vasiljević Branka, Opsenica, Dejan M., Milojković-opsenica Dušanka, Šenerović LidijaAbstract:Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts. Materials and methods: The anti-quorum sensing activity of Tn extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans leafs extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn leaf extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn leaf extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.Peer-reviewed manuscript: [http://cherry.chem.bg.ac.rs/handle/123456789/2932]Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/2933
Ivana Aleksic - One of the best experts on this subject based on the ideXlab platform.
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Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts.
Journal of ethnopharmacology, 2018Co-Authors: Ivana Aleksic, Ivana D. Radojević, Petar Ristivojević, Aleksandar Pavic, Ljiljana R. Čomić, Branka Vasiljevic, Dejan Opsenica, Dušanka Milojković-opsenica, Lidija SenerovicAbstract:Abstract Ethnopharmacological relevance Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts. Materials and methods The anti-quorum sensing activity of Tn extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans leafs extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results Subinhibitory concentrations of Tn leaf extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions This study demonstrates anti-virulence potential of Tn leaf extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.
Vinod Kumar - One of the best experts on this subject based on the ideXlab platform.
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Phytoremediation potential of water caltrop (Trapa natans L.) using municipal wastewater of the activated sludge process-based municipal wastewater treatment plant.
Environmental technology, 2017Co-Authors: Vinod Kumar, A K ChopraAbstract:ABSTRACTPhytoremediation experiments were carried out to assess the phytoremediation potential of water caltrop (Trapa natans L.) using municipal wastewater collected from the activated sludge process- (ASP) based municipal wastewater treatment plant. The results revealed that T. natans significantly (P ≤ .05/P ≤ .01/P ≤ .001) reduced the contents of total dissolved solids (TDS), electrical conductivity (EC), biochemical oxygen demand (BOD5), chemical oxygen demand, total Kjeldahl nitrogen, phosphate (), sodium (Na+), potassium (K+), calcium (Ca2+), magnesium (Mg2+), cadmium (Cd), chromium (Cr), copper (Cu), iron (Fe), manganese (Mn), lead (Pb), zinc (Zn), standard plate count, and most probable number of the municipal wastewater after phytoremediation experiments. The maximum removal of these parameters was obtained at 60 days of the phytoremediation experiments, but the removal rate of these parameters was gradually increased from 15 to 45 days and it was slightly decreased at 60 days. Most contents of ...
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Comparative assessment of phytoremediation feasibility of water caltrop (Trapa natans L.) and water hyacinth (Eichhornia crassipes Solms.) using pulp and paper mill effluent
2016Co-Authors: Vinod KumarAbstract:Experiments for the comparative assessment of phytoremediation feasibility of water caltrop (Trapa natans L.) and water hyacinth (Eichhornia crassipes Solms.) using paper mill effluent were carried out for 60 days. The results revealed that the pulp and paper mill effluent was varied in characteristics and highly loaded with TDS, EC, BOD5, COD, TKN, PO4 3- , Na+ , K+ , Ca2+, Mg2+, Cd, Cr, Cu, Fe, Mn, Ni, Pb, Zn, SPC and MPN. It was observed that and both the plant species T. natansand E. crassipes significantly (P
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reduction of pollution load of paper mill effluent by phytoremediation technique using water caltrop Trapa natans l
Cogent Environmental Science, 2016Co-Authors: Vinod Kumar, A K ChopraAbstract:AbstractLaboratory experiments were conducted to investigate the reduction of pollution load of paper mill effluent through phytoremediation technique using water caltrop (Trapa natans). Different concentrations, viz. 0% (control), 25, 50, 75, and 100% (absolute) were treated using T. natans for 60 days. Results showed that sewage effluent was loaded with various parameters, viz. total dissolved solids (TDS), electrical conductivity, total Kjeldhal nitrogen (TKN), phosphate (PO43-), calcium (Ca2+), magnesium (Mg2+), potassium (K+), cadmium (Cd), copper (Cu), iron (Fe), nickel (Ni), lead (Pb), and zinc (Zn). The maximum removal of different parameters was recorded after 60 days of phytoremediation experiments. Water caltrop (T. natans) significantly (p < 0.05/p < 0.01) removed TDS, BOD, COD, TKN, PO43-, Ca2+, Mg2+ and K+, Cd, Cu, Fe, Ni, Pb, and Zn of the paper mill effluent after the phytoremediation. The removal of different pollutants from the paper mill effluent was gradually increased at lower concent...
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Reduction of pollution load of paper mill effluent by phytoremediation technique using water caltrop (Trapa natans L.)
Cogent Environmental Science, 2016Co-Authors: Vinod Kumar, A K ChopraAbstract:AbstractLaboratory experiments were conducted to investigate the reduction of pollution load of paper mill effluent through phytoremediation technique using water caltrop (Trapa natans). Different concentrations, viz. 0% (control), 25, 50, 75, and 100% (absolute) were treated using T. natans for 60 days. Results showed that sewage effluent was loaded with various parameters, viz. total dissolved solids (TDS), electrical conductivity, total Kjeldhal nitrogen (TKN), phosphate (PO43-), calcium (Ca2+), magnesium (Mg2+), potassium (K+), cadmium (Cd), copper (Cu), iron (Fe), nickel (Ni), lead (Pb), and zinc (Zn). The maximum removal of different parameters was recorded after 60 days of phytoremediation experiments. Water caltrop (T. natans) significantly (p
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Reduction of pollution load of paper mill effluent by phytoremediation technique using water caltrop (Trapa natans L.)
Taylor & Francis Group, 2016Co-Authors: Vinod Kumar, A K ChopraAbstract:Laboratory experiments were conducted to investigate the reduction of pollution load of paper mill effluent through phytoremediation technique using water caltrop (Trapa natans). Different concentrations, viz. 0% (control), 25, 50, 75, and 100% (absolute) were treated using T. natans for 60 days. Results showed that sewage effluent was loaded with various parameters, viz. total dissolved solids (TDS), electrical conductivity, total Kjeldhal nitrogen (TKN), phosphate ($ {\text{PO}}_{4}^{3- } $), calcium (Ca2+), magnesium (Mg2+), potassium (K+), cadmium (Cd), copper (Cu), iron (Fe), nickel (Ni), lead (Pb), and zinc (Zn). The maximum removal of different parameters was recorded after 60 days of phytoremediation experiments. Water caltrop (T. natans) significantly (p
Aleksić Ivana - One of the best experts on this subject based on the ideXlab platform.
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Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts
Elsevier Ireland Ltd Clare, 2018Co-Authors: Aleksić Ivana, Ristivojević Petar, Pavić Aleksandar, Radojević Ivana, Čomić, Ljiljana R., Vasiljević Branka, Opsenica, Dejan M., Milojković-opsenica Dušanka, Šenerović LidijaAbstract:Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts. Materials and methods: The anti-quorum sensing activity of Tn extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans leafs extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn leaf extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn leaf extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.This is the peer-reviewed version of the following article: Aleksic, I.; Ristivojevic, P.; Pavic, A.; Radojević, I.; Čomić, L. R.; Vasiljevic, B.; Opsenica, D.; Milojković-Opsenica, D.; Senerovic, L. Anti-Quorum Sensing Activity, Toxicity in Zebrafish (Danio Rerio) Embryos and Phytochemical Characterization of Trapa natans Leaf Extracts. Journal of Ethnopharmacology 2018, 222, 148–158. [https://doi.org/10.1016/j.jep.2018.05.005]Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/2933
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Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts
'Elsevier BV', 2018Co-Authors: Aleksić Ivana, Ristivojević Petar, Pavić Aleksandar, Radojević Ivana, Čomić, Ljiljana R., Vasiljević Branka, Milojković-opsenica Dušanka, Opsenica Dejan, Šenerović LidijaAbstract:Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts. Materials and methods: The anti-quorum sensing activity of Tn extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans leafs extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn leaf extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn leaf extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.This is the peer-reviewed version of the following article: Aleksic, I.; Ristivojevic, P.; Pavic, A.; Radojević, I.; Čomić, L. R.; Vasiljevic, B.; Opsenica, D.; Milojković-Opsenica, D.; Senerovic, L. Anti-Quorum Sensing Activity, Toxicity in Zebrafish (Danio Rerio) Embryos and Phytochemical Characterization of Trapa natans Leaf Extracts. Journal of Ethnopharmacology 2018, 222, 148–158. [https://doi.org/10.1016/j.jep.2018.05.005][http://cer.ihtm.bg.ac.rs/handle/123456789/2310
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Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts
Elsevier Ireland Ltd Clare, 2018Co-Authors: Aleksić Ivana, Ristivojević Petar, Pavić Aleksandar, Vasiljević Branka, Milojković-opsenica Dušanka, Opsenica Dejan, Radojevic Ivana, Comic, Ljiljana R., Šenerović LidijaAbstract:Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts. Materials and methods: The anti-quorum sensing activity of Tn extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans leafs extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn leaf extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn leaf extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.The peer-reviewed version: [http://cer.ihtm.bg.ac.rs/handle/123456789/3139
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Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts
Elsevier Ireland Ltd Clare, 2018Co-Authors: Aleksić Ivana, Ristivojević Petar, Pavić Aleksandar, Radojević Ivana, Čomić, Ljiljana R., Vasiljević Branka, Opsenica, Dejan M., Milojković-opsenica Dušanka, Šenerović LidijaAbstract:Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts. Materials and methods: The anti-quorum sensing activity of Tn extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans leafs extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn leaf extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn leaf extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.Peer-reviewed manuscript: [http://cherry.chem.bg.ac.rs/handle/123456789/2932]Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/2933
