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Emerson Luiz Botelho Lourenço - One of the best experts on this subject based on the ideXlab platform.
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hydroethanolic extract of Tropaeolum majus promotes anxiolytic effects on rats
Revista Brasileira De Farmacognosia-brazilian Journal of Pharmacognosy, 2018Co-Authors: Ailton Da Cruz Melo, Emerson Luiz Botelho Lourenço, Simone Castagna Angelim Costa, Alanna Fernandes De Castro, Amanda Nascimento Vasques De Souza, Samantha Wietzikoski Sato, Francislaine A R Livero, Irineia Paulina Baretta, Evellyn Claudia Wietzikoski LovatoAbstract:Abstract Tropaeolum majus L., Tropaeolaceae, popularly known in Brazil as ‘capuchinha’ is widely used due its anti-inflammatory, antiseptic, anti-hypertensive and anti-depressive properties. However, scientific investigations about its effects on the central nervous system are still scarce. This study investigated the central pharmacological actions of the prolonged treatment with a hydroethanolic extract of T. majus in male Wistar rats in the elevated plus maze and hole-board behavioral models. For this, rats were daily treated with distillated water (negative control); diazepam (1 mg/kg) or hydroethanolic extract of T. majus (75, 150 and 300 mg/kg), for 29 days (by gavage) and were submitted to elevated plus maze and hole-board. Animals treated with all hydroethanolic extract of T. majus or diazepam doses increased the percentage of entries in open arms when compared to control group. However, only treatment with diazepam increased the length of time spent in the open arms of the elevated plus maze. No differences between all groups were observed regardless rearing, grooming, stretched-attend postures and defecation rates. In the HB test, in opposite to diazepam, treatment with hydroethanolic extract of T. majus did not interfere in the exploratory activity of rats. The hydroethanolic extract of T. majus promotes anxiolytic-like effects when orally administered in rats.
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fetopathies associated with exposure to angiotensin converting enzyme inhibitor from Tropaeolum majus l
Drug and Chemical Toxicology, 2017Co-Authors: Emerson Luiz Botelho Lourenço, Anderson Joel Martinoandrade, Paulo Roberto Dalsenter, Rita De Cassia Lima Ribeiro, Valdinei De Oliveira Araujo, Arquimedes GasparottoAbstract:AbstractThe prevalence of the use of herbal medicines is on the rise across the world, especially amongst pregnant women. A fact that draws attention is that many species commonly used by pregnant women, including the Tropaeolum majus L. (Tropaeolaceae), also present inhibitory activity on the angiotensin-converting enzyme (ACE). Herein, we have investigated the effects of T. majus extract (HETM) on fetal development, evaluating its relationship with possible ACE inhibitory activity. Pregnant Wistar rats were treated with different HETM doses (3, 30 and 300 mg/kg/day) from gestational days 8–20. Rats were sacrificed on the day 20 of pregnancy and the following parameters were evaluated: clinical symptoms of maternal toxicity; maternal body weight; feed and water intake; maternal liver, kidney, and ovary weights, maternal ACE activity and aldosterone levels, live fetuses mean; dead fetuses percentage, fetus weight, and fetal malformation. All pregnant rats treated with high HETM doses showed significant re...
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protective cardiorenal effects of Tropaeolum majus l in rats with renovascular hypertension
Journal of Young Pharmacists, 2017Co-Authors: Arquimedes Gasparotto, Francielly Mourao Gasparotto, Thiago Bruno Lima Prando, Karimi Sater Gebara, Francislaine Aparecida Dos Reis Livero, Denis Pires De Lima, Roberto Da Silva Gomes, Emerson Luiz Botelho LourençoAbstract:Background: Tropaeolum majus L. (Tropaeolaceae) is an important medicinal plant belonging to the Tropaeolaceae family, which has 80 species distributed in South and Central America. In Brazil, it is distributed practically throughout the entire territory and it is popularly known as “capuchinha”. In folk medicine its leaves are widely used for treatment of cardiovascular disorders. Despite consistent data showing some acute effects on renal and cardiovascular system, no study has investigated whether Tropaeolum majus extracts is able to exert cardiorenal protective effects after 30 days of treatment, i.e. in a sustained manner. Objective: Investigate the prolonged cardiorenal protective effects of ethanolic extract obtained from Tropaeolum majus (EETM) in rats with renovascular hypertension. Material and Methods: First, EETM was obtained and chemically characterized. Then, we investigated the possible antihypertensive and diuretics effects, including effects on renal haemodynamics, after prolonged treatment (30 days) with EETM (3, 30 and 300 mg/kg). Finally, we examined whether treatment with EETM may affect the angiotensin-converting enzyme (ACE) and oxidative stress, preventing heart and kidney damage in two-kidney, one-clip (2K1C) Goldblatt hypertensive rats. Results: Prolonged treatment with EETM was able to prevent the evolution of renovascular hypertension in 2K1C rats, inducing important renoprotective effects and reducing systemic blood pressure and cardiac hypertrophy. Moreover, it was also identified that these effects may be directly related to significant ACE inhibitory activity and reduction of oxidative stress. Conclusion: This study has brought new scientific evidence of preclinical efficacy of EETM as a cardiorenal protective agent in rats with renovascular hypertension. Key words: Antihypertensive, Cardioprotective, Diuretic, Renoprotective, Tropaeolum majus L.
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Safety Assessment and Botanical Standardization of an Edible Species from South America.
Journal of Medicinal Food, 2017Co-Authors: Giseli Karenina Traesel, Emerson Luiz Botelho Lourenço, Francislaine Aparecida Dos Reis Livero, Camila Dias Machado, Cleide Adriane Signor Tirloni, Jacqueline Vergutz Menetrier, Silvia Aparecida Oesterreich, Jane Manfron Budel, Arquimedes Gasparotto JuniorAbstract:Abstract Tropaeolum majus L. (Tropaeolaceae), commonly known as nasturtium, is an important edible plant native to the Andean States and widely disseminated throughout South America. Despite the use of this species is quite widespread, there are no minimum quality control standards or data on its genotoxicity. So, the aim of this study was to present a detailed anatomical and histochemical study for Tropaeolum majus and provide genotoxicity parameters of a preparation routinely used in South American countries. First, three different Tropaeolum majus aqueous extracts (TMAEs) at concentrations of 1.5%, 7%, and 15% were prepared according to the popular use. Then, genetic toxicity of TMAE was evaluated on bacterial reverse mutation, genomic lesions, and micronucleus formation in male rats. Furthermore, a detailed anatomical and histochemical study of the leaves and stems of Tropaeolum majus were performed. No revertant colonies were found in any bacterial cultures examined. In the comet assay, TMAE showed n...
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levantamento de informacoes etnobotânicas etnofarmacologicas e farmacologicas registradas na literatura sobre Tropaeolum majus l chaguinha
Arquivos de Ciências da Saúde da UNIPAR, 2017Co-Authors: Andressa Zardo, Arquimedes Gasparotto, Emerson Luiz Botelho Lourenço, Joice Karina Otenio, Ezilda JacomassiAbstract:O uso popular de plantas e uma arte muito antiga fundamentada no acumulo de informacoes repassadas por meio de sucessivas geracoes. A etnobotânica e a etnofarmacologia sao considerados ferramentas importantes no resgate de saberes tradicionais das sociedades humanas, passadas e presentes, e suas interacoes com as plantas e a geracao de conhecimento cientifico e tecnologico voltados para o uso sustentavel dos recursos naturais, e estao diretamente ligados com o mercado fitoterapico. A Tropaeolum majus L. conhecida popularmente como capuchinha, chaguinha ou nasturcio pertence a familia Tropaeolaceae e uma especie amplamente cultivada no Brasil, tanto para fins ornamentais, medicinais e alimenticios. A Empresa Brasileira de Pesquisa Agropecuaria (EMBRAPA) orienta e recomenda o cultivo desta especie para fins alimentares e medicinais. Diante dessa realidade, inumeros estudos foram realizados com essa especie no intuito de comprovar sua eficacia, seguranca e qualidade. A partir do levantamento bibliografico sobre a etnobotânica, etnofarmacologia, farmacologia e toxicologia da especie em questao, observou-se que existem varios trabalhos publicados que justificam a utilizacao desta especie, tanto no uso medicinal quanto alimenticio, podendo-se lancar no mercado de produtos naturais um novo fitoterapico promissor, cujos estudos encontram-se bem definidos e justificados.
Arquimedes Gasparotto - One of the best experts on this subject based on the ideXlab platform.
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90 day oral toxicity assessment of Tropaeolum majus l in rodents and lagomorphs
Journal of Medicinal Food, 2018Co-Authors: Valdinei De Oliveira Araujo, Caroline Gomes, Carlos Eduardo Linhares Andreotti, Michelle De Paula Reis, Daniely Alves De Lima, Karoline Bach Pauli, Bruna Caroline Nunes, Ricardo De Melo Germano, Euclides Lara Cardozo, Arquimedes GasparottoAbstract:Abstract Tropaeolum majus L., popularly known as nasturtium, is a species widely used in the form of infusions and salads. In the last years, the antihypertensive, diuretic, and calcium and potassium sparing activities of T. majus preparations were shown. Moreover, no preclinical 90-day oral toxicity studies were conducted. Thus, this study evaluated the toxicity of the hydroethanolic extract obtained from T. majus (HETM) leaves in female and male mice, rats, and rabbits. Swiss mice and Wistar rats were treated with HETM (75, 375, and 750 mg/kg). The doses of rabbits (30, 150, and 300 mg/kg) were calculated by allometric extrapolation. The control groups received vehicle. The animals were orally treated, daily, for 90 days. At the end, the animals were anesthetized, and body weight gain, relative weight of liver, kidney, and spleen, and histopathological changes were evaluated. Serum hematological and biochemical parameters were also analyzed. No alterations were found in body and organ weights or in hist...
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fetopathies associated with exposure to angiotensin converting enzyme inhibitor from Tropaeolum majus l
Drug and Chemical Toxicology, 2017Co-Authors: Emerson Luiz Botelho Lourenço, Anderson Joel Martinoandrade, Paulo Roberto Dalsenter, Rita De Cassia Lima Ribeiro, Valdinei De Oliveira Araujo, Arquimedes GasparottoAbstract:AbstractThe prevalence of the use of herbal medicines is on the rise across the world, especially amongst pregnant women. A fact that draws attention is that many species commonly used by pregnant women, including the Tropaeolum majus L. (Tropaeolaceae), also present inhibitory activity on the angiotensin-converting enzyme (ACE). Herein, we have investigated the effects of T. majus extract (HETM) on fetal development, evaluating its relationship with possible ACE inhibitory activity. Pregnant Wistar rats were treated with different HETM doses (3, 30 and 300 mg/kg/day) from gestational days 8–20. Rats were sacrificed on the day 20 of pregnancy and the following parameters were evaluated: clinical symptoms of maternal toxicity; maternal body weight; feed and water intake; maternal liver, kidney, and ovary weights, maternal ACE activity and aldosterone levels, live fetuses mean; dead fetuses percentage, fetus weight, and fetal malformation. All pregnant rats treated with high HETM doses showed significant re...
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protective cardiorenal effects of Tropaeolum majus l in rats with renovascular hypertension
Journal of Young Pharmacists, 2017Co-Authors: Arquimedes Gasparotto, Francielly Mourao Gasparotto, Thiago Bruno Lima Prando, Karimi Sater Gebara, Francislaine Aparecida Dos Reis Livero, Denis Pires De Lima, Roberto Da Silva Gomes, Emerson Luiz Botelho LourençoAbstract:Background: Tropaeolum majus L. (Tropaeolaceae) is an important medicinal plant belonging to the Tropaeolaceae family, which has 80 species distributed in South and Central America. In Brazil, it is distributed practically throughout the entire territory and it is popularly known as “capuchinha”. In folk medicine its leaves are widely used for treatment of cardiovascular disorders. Despite consistent data showing some acute effects on renal and cardiovascular system, no study has investigated whether Tropaeolum majus extracts is able to exert cardiorenal protective effects after 30 days of treatment, i.e. in a sustained manner. Objective: Investigate the prolonged cardiorenal protective effects of ethanolic extract obtained from Tropaeolum majus (EETM) in rats with renovascular hypertension. Material and Methods: First, EETM was obtained and chemically characterized. Then, we investigated the possible antihypertensive and diuretics effects, including effects on renal haemodynamics, after prolonged treatment (30 days) with EETM (3, 30 and 300 mg/kg). Finally, we examined whether treatment with EETM may affect the angiotensin-converting enzyme (ACE) and oxidative stress, preventing heart and kidney damage in two-kidney, one-clip (2K1C) Goldblatt hypertensive rats. Results: Prolonged treatment with EETM was able to prevent the evolution of renovascular hypertension in 2K1C rats, inducing important renoprotective effects and reducing systemic blood pressure and cardiac hypertrophy. Moreover, it was also identified that these effects may be directly related to significant ACE inhibitory activity and reduction of oxidative stress. Conclusion: This study has brought new scientific evidence of preclinical efficacy of EETM as a cardiorenal protective agent in rats with renovascular hypertension. Key words: Antihypertensive, Cardioprotective, Diuretic, Renoprotective, Tropaeolum majus L.
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levantamento de informacoes etnobotânicas etnofarmacologicas e farmacologicas registradas na literatura sobre Tropaeolum majus l chaguinha
Arquivos de Ciências da Saúde da UNIPAR, 2017Co-Authors: Andressa Zardo, Arquimedes Gasparotto, Emerson Luiz Botelho Lourenço, Joice Karina Otenio, Ezilda JacomassiAbstract:O uso popular de plantas e uma arte muito antiga fundamentada no acumulo de informacoes repassadas por meio de sucessivas geracoes. A etnobotânica e a etnofarmacologia sao considerados ferramentas importantes no resgate de saberes tradicionais das sociedades humanas, passadas e presentes, e suas interacoes com as plantas e a geracao de conhecimento cientifico e tecnologico voltados para o uso sustentavel dos recursos naturais, e estao diretamente ligados com o mercado fitoterapico. A Tropaeolum majus L. conhecida popularmente como capuchinha, chaguinha ou nasturcio pertence a familia Tropaeolaceae e uma especie amplamente cultivada no Brasil, tanto para fins ornamentais, medicinais e alimenticios. A Empresa Brasileira de Pesquisa Agropecuaria (EMBRAPA) orienta e recomenda o cultivo desta especie para fins alimentares e medicinais. Diante dessa realidade, inumeros estudos foram realizados com essa especie no intuito de comprovar sua eficacia, seguranca e qualidade. A partir do levantamento bibliografico sobre a etnobotânica, etnofarmacologia, farmacologia e toxicologia da especie em questao, observou-se que existem varios trabalhos publicados que justificam a utilizacao desta especie, tanto no uso medicinal quanto alimenticio, podendo-se lancar no mercado de produtos naturais um novo fitoterapico promissor, cujos estudos encontram-se bem definidos e justificados.
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prolonged diuretic activity and calcium sparing effect of Tropaeolum majus evidence in the prevention of osteoporosis
Evidence-based Complementary and Alternative Medicine, 2014Co-Authors: Lorena Neris Barboza, Francielly Mourao Gasparotto, Emerson Luiz Botelho Lourenço, Thiago Bruno Lima Prando, Paulo Roberto Dalsenter, Valdinei De Oliveira Araujo, Ezilda Jacomassi, Arquimedes GasparottoAbstract:Although several studies indicate high effectiveness in the use of the hydroethanolic extract from Tropaeolum majus (HETM) as a diuretic, the impact of its prolonged use in the presence of low estrogen levels remains unclear. Thus, the aim of this study was to investigate the diuretic effects of prolonged administration of HETM in ovariectomized rats and their interrelationship between calcium excretion and bone turnover. Forty-two female Wistar rats were ovariectomized (OVX) and treated orally with different doses of HETM (3, 30, and 300 mg/kg) for 4 weeks. On the first day of treatment and at weekly intervals for four weeks the diuretic activity was evaluated. Electrolyte concentrations and creatinine levels were estimated from urine sample of each rat. The serum lipids, urea, creatinine, and osteocalcin were also measured at the end of the experiment. The data revealed that the HETM was able to sustain its diuretic effect after prolonged treatment. Moreover, its use has not affected the urinary calcium or potassium excretion, reduces lipid levels, and maintains osteocalcin levels similarly to untreated rats. These findings support the potential of HETM as a candidate to be used in clinical conditions in which the renal loss of calcium is not desired.
Vladimir Farkas - One of the best experts on this subject based on the ideXlab platform.
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Engineering the acceptor substrate specificity in the xyloglucan endotransglycosylase TmXET6.3 from nasturtium seeds (Tropaeolum majus L.).
Plant molecular biology, 2019Co-Authors: Barbora Stratilová, Zuzana Firáková, Jaroslav Klaudiny, Sergej Šesták, Stanislav Kozmon, Dana Strouhalová, Soňa Garajová, Fairouz Ait-mohand, Ágnes Horváthová, Vladimir FarkasAbstract:The knowledge of substrate specificity of XET enzymes is important for the general understanding of metabolic pathways to challenge the established notion that these enzymes operate uniquely on cellulose-xyloglucan networks. Xyloglucan xyloglucosyl transferases (XETs) (EC 2.4.1.207) play a central role in loosening and re-arranging the cellulose-xyloglucan network, which is assumed to be the primary load-bearing structural component of plant cell walls. The sequence of mature TmXET6.3 from Tropaeolum majus (280 residues) was deduced by the nucleotide sequence analysis of complete cDNA by Rapid Amplification of cDNA Ends, based on tryptic and chymotryptic peptide sequences. Partly purified TmXET6.3, expressed in Pichia occurred in N-glycosylated and unglycosylated forms. The quantification of hetero-transglycosylation activities of TmXET6.3 revealed that (1,3;1,4)-, (1,6)- and (1,4)-β-D-glucooligosaccharides were the preferred acceptor substrates, while (1,4)-β-D-xylooligosaccharides, and arabinoxylo- and glucomanno-oligosaccharides were less preferred. The 3D model of TmXET6.3, and bioinformatics analyses of identified and putative plant xyloglucan endotransglycosylases (XETs)/hydrolases (XEHs) of the GH16 family revealed that H94, A104, Q108, K234 and K237 were the key residues that underpinned the acceptor substrate specificity of TmXET6.3. Compared to the wild-type enzyme, the single Q108R and K237T, and double-K234T/K237T and triple-H94Q/A104D/Q108R variants exhibited enhanced hetero-transglycosylation activities with xyloglucan and (1,4)-β-D-glucooligosaccharides, while those with (1,3;1,4)- and (1,6)-β-D-glucooligosaccharides were suppressed; the incorporation of xyloglucan to (1,4)-β-D-glucooligosaccharides by the H94Q variant was influenced most extensively. Structural and biochemical data of non-specific TmXET6.3 presented here extend the classic XET reaction mechanism by which these enzymes operate in plant cell walls. The evaluations of TmXET6.3 transglycosylation activities and the incidence of investigated residues in other members of the GH16 family suggest that a broad acceptor substrate specificity in plant XET enzymes could be more widespread than previously anticipated.
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screening for hetero transglycosylating activities in extracts from nasturtium Tropaeolum majus
Carbohydrate Research, 2006Co-Authors: Fairouz Ait Mohand, Vladimir FarkasAbstract:Using combinations of different polysaccharides as glycosyl donors and of oligosaccharides fluorescently labeled by sulforhodamine (SR) as glycosyl acceptors, we screened for the presence of transglycosylating activities in extracts from nasturtium (Tropaeolum majus). Besides xyloglucan endotransglycosylase/hydrolase (XTH/XET, EC 2.4.1.207) activity, which transfers xyloglucanosyl residues from xyloglucan (XG) to XG-derived oligosaccharides (XGOs), a glycosyl transfer from XG to SR-labeled cellooligosaccharides and laminarioligosaccharides has been detected. The XGOs also served as acceptors for the glycosyl transfer from soluble cellulose derivatives carboxymethyl cellulose and hydroxyethylcellulose. The effectivity of these polysaccharides as glycosyl donors for transfer to XG-derived octasaccharide [1-3H]XXLGol decreased in the order XG > HEC > CMC. Isoelectric focusing in polyacrylamide gels showed that bands corresponding to hetero-transglycosylase activities coincided with zones corresponding to XTH/XET. These results can be explained as due either to substrate non-specificity of certain isoenzymes of XTH/XET or to existence of enzymes catalyzing a hetero-transfer, that is the formation of covalent linkages between different types of carbohydrate polymers.
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divergent modes of action on xyloglucan of two isoenzymes of xyloglucan endo transglycosylase from Tropaeolum majus
Plant Physiology and Biochemistry, 2003Co-Authors: Zdena Sulova, Richard Baran, Vladimir FarkasAbstract:Abstract Two isoenzymes of xyloglucan endo-transglycosylase (XET, EC 2.4.1.207) were identified in nasturtium (Tropaeolum majus L.), so far. One is located in seeds (sXET) and is expressed during germination. The other enzyme (eXET) is confined to epicotyls and other growing regions. In this work, we examined catalytic properties of the two XETs and tried to find a correlation with their presumed functions. The two enzymes had similar isoelectric points at about pH 6.5 but had different pH-activity profiles and differed in Km values for xyloglucan-derived oligosaccharides (XGOS) as acceptor substrates. Moreover, they showed clearly distinct preferences in selecting the site of attack on xyloglucan (XG) molecules. While sXET selected the site of cleavage on XG molecules stochastically along the length of their polyglucose main chain and preferred low-molecular mass (MM) XGOS as glycosyl acceptors, eXET attacked the substrate molecule predominantly near the reducing end and showed no preference as to the size of XGOS acceptors. These properties corroborate well with the proposed functions of the two isoenzymes: the sXET plays a role in degrading XG reserves in seeds during germination, whereas the eXET is engaged in cell wall rearrangement and integration of new XG molecules into the preexisting cell wall structure during growth.
Jacob Kruger Jensen - One of the best experts on this subject based on the ideXlab platform.
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rna seq analysis of developing nasturtium seeds Tropaeolum majus identification and characterization of an additional galactosyltransferase involved in xyloglucan biosynthesis
Molecular Plant, 2012Co-Authors: Jacob Kruger Jensen, Alex Schultink, Kenneth Keegstra, Curtis G Wilkerson, Markus PaulyAbstract:A deep-sequencing approach was pursued utilizing 454 and Illumina sequencing methods to discover new genes involved in xyloglucan biosynthesis. cDNA sequences were generated from developing nasturtium (Tropaeolum majus) seeds, which produce large amounts of non-fucosylated xyloglucan as a seed storage polymer. In addition to known xyloglucan biosynthetic genes, a previously uncharacterized putative xyloglucan galactosyltransferase was identified. Analysis of an Arabidopsis thaliana mutant line defective in the corresponding ortholog (AT5G62220) revealed that this gene shows no redundancy with the previously characterized xyloglucan galactosyltransferase, MUR3, but is required for galactosyl-substitution of xyloglucan at a different position. The gene was termed XLT2 for Xyloglucan L-side chain galactosylTransferase position 2. It represents an enzyme in the same subclade of glycosyltransferase family 47 as MUR3. A double mutant defective in both MUR3 (mur3.1) and XLT2 led to an Arabidopsis plant with xyloglucan that consists essentially of only xylosylated glucosyl units, with no further substitutions.
Paulo Roberto Dalsenter - One of the best experts on this subject based on the ideXlab platform.
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fetopathies associated with exposure to angiotensin converting enzyme inhibitor from Tropaeolum majus l
Drug and Chemical Toxicology, 2017Co-Authors: Emerson Luiz Botelho Lourenço, Anderson Joel Martinoandrade, Paulo Roberto Dalsenter, Rita De Cassia Lima Ribeiro, Valdinei De Oliveira Araujo, Arquimedes GasparottoAbstract:AbstractThe prevalence of the use of herbal medicines is on the rise across the world, especially amongst pregnant women. A fact that draws attention is that many species commonly used by pregnant women, including the Tropaeolum majus L. (Tropaeolaceae), also present inhibitory activity on the angiotensin-converting enzyme (ACE). Herein, we have investigated the effects of T. majus extract (HETM) on fetal development, evaluating its relationship with possible ACE inhibitory activity. Pregnant Wistar rats were treated with different HETM doses (3, 30 and 300 mg/kg/day) from gestational days 8–20. Rats were sacrificed on the day 20 of pregnancy and the following parameters were evaluated: clinical symptoms of maternal toxicity; maternal body weight; feed and water intake; maternal liver, kidney, and ovary weights, maternal ACE activity and aldosterone levels, live fetuses mean; dead fetuses percentage, fetus weight, and fetal malformation. All pregnant rats treated with high HETM doses showed significant re...
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prolonged diuretic activity and calcium sparing effect of Tropaeolum majus evidence in the prevention of osteoporosis
Evidence-based Complementary and Alternative Medicine, 2014Co-Authors: Lorena Neris Barboza, Francielly Mourao Gasparotto, Emerson Luiz Botelho Lourenço, Thiago Bruno Lima Prando, Paulo Roberto Dalsenter, Valdinei De Oliveira Araujo, Ezilda Jacomassi, Arquimedes GasparottoAbstract:Although several studies indicate high effectiveness in the use of the hydroethanolic extract from Tropaeolum majus (HETM) as a diuretic, the impact of its prolonged use in the presence of low estrogen levels remains unclear. Thus, the aim of this study was to investigate the diuretic effects of prolonged administration of HETM in ovariectomized rats and their interrelationship between calcium excretion and bone turnover. Forty-two female Wistar rats were ovariectomized (OVX) and treated orally with different doses of HETM (3, 30, and 300 mg/kg) for 4 weeks. On the first day of treatment and at weekly intervals for four weeks the diuretic activity was evaluated. Electrolyte concentrations and creatinine levels were estimated from urine sample of each rat. The serum lipids, urea, creatinine, and osteocalcin were also measured at the end of the experiment. The data revealed that the HETM was able to sustain its diuretic effect after prolonged treatment. Moreover, its use has not affected the urinary calcium or potassium excretion, reduces lipid levels, and maintains osteocalcin levels similarly to untreated rats. These findings support the potential of HETM as a candidate to be used in clinical conditions in which the renal loss of calcium is not desired.
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effects of angiotensin converting enzyme inhibitor derived from Tropaeolum majus l in rat preimplantation embryos evidence for the dehydroepiandrosterone and estradiol role
Evidence-based Complementary and Alternative Medicine, 2014Co-Authors: Emerson Luiz Botelho Lourenço, Arquimedes Gasparotto, Thiago Bruno Lima Prando, Juliane Centeno Muller, Ana Claudia Boareto, Caroline Gomes, Ana Carolina Lourenco, Rhanany Alan Calloi Palozi, Paulo Roberto DalsenterAbstract:Although several studies have shown the inhibitory effects of Tropaeolum majus extracts (HETM) on angiotensin-converting enzyme (ACE) activity, no studies have been carried out during the beginning of pregnancy, when humoral and hormonal imbalance may affect zygote and early embryo transport. This study investigates whether HETM can affect embryonic development when administered during the one-cell-blastocyst period. Pregnant Wistar rats received orally the HETM (3, 30, and 300 mg/kg/day) from the 1st to the 7th gestational day. Rats were killed on the 8th day of pregnancy and the following parameters were evaluated: clinical symptoms of toxicity (including organ weights), number of corpora lutea, implants per group, preimplantation losses ratio, and the serum levels of dehydroepiandrosterone (DHEA), estradiol, and progesterone. No clinical symptoms of maternal toxicity were evidenced. On the 8th day of pregnancy, the levels of DHEA and estradiol were increased and significant preimplantation losses were observed at all doses used. The present study reveals that the HETM can raise levels of DHEA and estradiol and induce difficulty in the embryo implantation in the early stages of pregnancy. The data contributes significantly to the safety aspects of using this natural product when trying to get pregnant or during pregnancy.
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evaluation of subchronic toxicity of the hydroethanolic extract of Tropaeolum majus in wistar rats
Journal of Ethnopharmacology, 2012Co-Authors: Caroline Gomes, Arquimedes Gasparotto, Emerson Luiz Botelho Lourenço, Ana Carolina Lourenco, Erica Barbosa Liuti, Aline Oliveira Duque, Fabiola Nihi, Tatiane Camacho Mendes, Paulo Roberto DalsenterAbstract:Abstract Ethnopharmacological relevance The use of medicinal plants for the treatment of diseases usually comes from the belief that they present low toxicity due their natural origin. However, it is necessary a toxicological and pharmacological evaluation for these plants. Tropaeolum majus is a medicinal plant used in popular medicine to treat several diseases, including cardiovascular disorders, urinary tract infections and asthma. Even though several studies proved its therapeutic effects, there are few toxicological studies with this species. Aim of the study The present study was carried out to evaluate the subchronic toxicity of the hydroethanolic extract obtained from leaves of T. majus (HETM) in Wistar rats. Material and methods Male and female Wistar rats received three doses of HETM (75, 375 and 750 mg/kg) for 28 days. After the treatments biochemical, hematological and histopathological parameters were analyzed. Results No significant alterations in the animal's body weight gain, relative organs weight, serum biochemical analysis, hematological or histopathological analyses of liver, kidneys and spleen were observed. Conclusions These results demonstrate the absence of subchronic toxicity due to oral treatment with HETM for 28 days in Wistar rats. However, other toxicological studies are necessary to evaluate the total safety of this plant.
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screening for in vivo anti estrogenic and anti androgenic activities of Tropaeolum majus l and its effect on uterine contractility
Journal of Ethnopharmacology, 2012Co-Authors: Emerson Luiz Botelho Lourenço, Arquimedes Gasparotto, Juliane Centeno Muller, Ana Claudia Boareto, Caroline Gomes, Ana Carolina Lourenco, Bruna Minatovicz, Sandra Crestani, Anderson Joel Martinoandrade, Paulo Roberto DalsenterAbstract:Abstract Ethnopharmacological relevance Tropaeolum majus L. (Tropaeolaceae) is a medicinal herb popularly used in Brazil for treatment of inflammatory and cardiovascular diseases. Despite some published data on its efficacy, there are still few toxicological data describing the safety of this plant. The aim of this study was to evaluate the (anti)estrogenic and (anti)androgenic activity of the hydroethanolic extract obtained from Tropaeolum majus L. (HETM), as well as its possible effects on uterine contractility. Materials and methods Three experimental protocols were performed, (a) uterotrophic assay, (b) Hershberger assay and (c) an ex vivo test to investigate the effects of maternal administration of HETM on uterine contractility at the end of pregnancy. In all protocols three doses of the HETM were administered to Wistar rats: 3, 30 and 300 mg/kg. Results In vivo tests for detection of (anti)androgenic and (anti)estrogenic activities did not show any significant alterations. Similarly, no alterations were observed on uterine contractility induced by oxytocin and arachidonic acid. Conclusions HETM was unable to produce (anti)estrogenic or (anti)androgenic activities in the short-term in vivo screening assays performed. In addition, there was no evidence that HETM can affect uterine contractility following gestational exposure of rats.
