The Experts below are selected from a list of 531 Experts worldwide ranked by ideXlab platform
Hideaki Kakeya - One of the best experts on this subject based on the ideXlab platform.
-
longicatenamides a d two diastereomeric pairs of cyclic hexapeptides produced by combined culture of streptomyces sp kusc_f05 and Tsukamurella pulmonis tp b0596
The Journal of Antibiotics, 2021Co-Authors: Yulu Jiang, Hiroyasu Onaka, Takefumi Kuranaga, Takumi Matsumoto, Weicheng Wang, Hideaki KakeyaAbstract:Longicatenamides A-D, two diastereomeric pairs of new cyclic hexapeptides, were isolated from the combined-culture of Streptomyces sp. KUSC_F05 and Tsukamurella pulmonis TP-B0596. Their planar structures were determined by spectroscopic analysis including extensive 2D NMR and MS analysis. The absolute configurations of their component amino acids were determined by the use of highly sensitive reagents we recently developed; the highly sensitive-advanced Marfey's method (HS-advanced Marfey's method), which led us to reduce the sample loss and prevent incorrect structural determination. Particularly, the Cβ-stereochemistry of hyGlu in longicatenamides A and C was assigned without any use of Cβ-Marfey's methods. Longicatenamide A exhibited weak but preferential antimicrobial activity against Bacillus subtilis.
-
amycolapeptins a and b cyclic nonadepsipeptides produced by combined culture of amycolatopsis sp and Tsukamurella pulmonis
Journal of Organic Chemistry, 2021Co-Authors: Chengqian Pan, Hiroyasu Onaka, Takefumi Kuranaga, Xun Cao, Takehiro Suzuki, Naoshi Dohmae, Naoya Shinzato, Hideaki KakeyaAbstract:Two nonapeptide natural products, amycolapeptins A (1) and B (2) with a 22-membered cyclic depsipeptide skeleton, β-hydroxytyrosine, and a highly modified side chain, which were not produced in a monoculture of the rare actinomycete Amycolatopsis sp. 26-4, were discovered in broth of its combined-culture with Tsukamurella pulmonis TP-B0596. The planar structures were elucidated by spectroscopic analyses (extensive 2D-NMR and MALDI-TOF MS/MS). The absolute configurations of component amino acids were unambiguously determined by the highly sensitive advanced Marfey's method we recently developed. Additionally, the structures of unstable/unusual moieties were corroborated by chemical synthesis and CD analysis.
-
enhancement of saccharothriolide production and discovery of a new metabolite saccharothriolide c2 by combined culture of saccharothrix sp and Tsukamurella pulmonis
Tetrahedron Letters, 2019Co-Authors: Yulu Jiang, Hiroyasu Onaka, Go Hirai, Taira Kato, Hideaki KakeyaAbstract:Abstract A combined-culture method was applied to saccharothriolide-producing strain Saccharothrix sp. A1506 with the mycolic acid-containing bacterium (MACB) Tsukamurella pulmonis TP-B0596. The combined-culture not only enhanced saccharothriolide production, but also produced saccharothriolide C2, a new C-2 epimer of saccharothriolide C, thereby indicating its potential for increasing the chemical diversity of microbial metabolites.
-
discovery and total synthesis of streptoaminals antimicrobial 5 5 spirohemiaminals from the combined culture of streptomyces nigrescens and Tsukamurella pulmonis
ChemInform, 2016Co-Authors: Ryosuke Sugiyama, Hiroyasu Onaka, Shumpei Asamizu, Shinichi Nishimura, Taro Ozaki, Hideaki KakeyaAbstract:A series of lipidic spirohemiaminals, designated streptoaminals, is reported. These were discovered by surveying the unique molecular signatures identified in the mass spectrometry data of the combined-culture broth of Streptomyces nigrescens HEK616 and Tsukamurella pulmonis TP-B0596. Mass spectrometry analysis showed that streptoaminals appeared as a cluster of ion peaks, which were separated by 14 mass unit intervals, implying the presence of alkyl chains of different lengths. The chemical structures of these compounds were elucidated by spectroscopic analysis and total synthesis. Streptoaminals with globular structures showed broad antimicrobial activities, whereas the planar structures of the 5-alkyl-1,2,3,4-tetrahydroquinolines found in the same combined-culture did not. This work shows the application of microbes as reservoirs for a range of chemical scaffolds.
-
5 alkyl 1 2 3 4 tetrahydroquinolines new membrane interacting lipophilic metabolites produced by combined culture of streptomyces nigrescens and Tsukamurella pulmonis
Organic Letters, 2015Co-Authors: Ryosuke Sugiyama, Hiroyasu Onaka, Shumpei Asamizu, Shinichi Nishimura, Taro Ozaki, Hideaki KakeyaAbstract:Eight novel 5-alkyl-1,2,3,4-tetrahydroquinolines (5aTHQs) bearing different side chains have been isolated from a combined culture of Streptomyces nigrescens HEK616 and Tsukamurella pulmonis TP-B0596. The chemical structures including the absolute configuration were elucidated by spectroscopic analysis and total synthesis. 5aTHQs inhibited the growth of wild-type fission yeast while only weakly inhibiting the growth of several mutant strains synthesizing premature ergosterol. These results demonstrate that 5aTHQs are novel antifungals that may target cell membranes.
Hiroyasu Onaka - One of the best experts on this subject based on the ideXlab platform.
-
nmr characterization of streptogramin b and l 156 587 a non synergistic pair of the streptogramin family antibiotic complexes produced inductively by a combined culture of streptomyces albogriseolus and Tsukamurella pulmonis
Magnetic Resonance in Chemistry, 2021Co-Authors: Naoya Oku, Hiroyasu Onaka, Shion Takemura, Yasuhiro IgarashiAbstract:The complete 1 H and 13 C NMR characterization of streptogramin B (1), the major component of a clinically important synergistic antibiotic complex, was presented for the first time, along with those of L-156,587 (2), a dehydrated congener of streptogramin A (3). Compounds 1 and 2 were not synergistic and produced by Streptomyces albogriseolus in co-culture with Tsukamurella pulmonis, which poses a question on the adaptive significance of the induced production of this antibiotic pair.
-
longicatenamides a d two diastereomeric pairs of cyclic hexapeptides produced by combined culture of streptomyces sp kusc_f05 and Tsukamurella pulmonis tp b0596
The Journal of Antibiotics, 2021Co-Authors: Yulu Jiang, Hiroyasu Onaka, Takefumi Kuranaga, Takumi Matsumoto, Weicheng Wang, Hideaki KakeyaAbstract:Longicatenamides A-D, two diastereomeric pairs of new cyclic hexapeptides, were isolated from the combined-culture of Streptomyces sp. KUSC_F05 and Tsukamurella pulmonis TP-B0596. Their planar structures were determined by spectroscopic analysis including extensive 2D NMR and MS analysis. The absolute configurations of their component amino acids were determined by the use of highly sensitive reagents we recently developed; the highly sensitive-advanced Marfey's method (HS-advanced Marfey's method), which led us to reduce the sample loss and prevent incorrect structural determination. Particularly, the Cβ-stereochemistry of hyGlu in longicatenamides A and C was assigned without any use of Cβ-Marfey's methods. Longicatenamide A exhibited weak but preferential antimicrobial activity against Bacillus subtilis.
-
amycolapeptins a and b cyclic nonadepsipeptides produced by combined culture of amycolatopsis sp and Tsukamurella pulmonis
Journal of Organic Chemistry, 2021Co-Authors: Chengqian Pan, Hiroyasu Onaka, Takefumi Kuranaga, Xun Cao, Takehiro Suzuki, Naoshi Dohmae, Naoya Shinzato, Hideaki KakeyaAbstract:Two nonapeptide natural products, amycolapeptins A (1) and B (2) with a 22-membered cyclic depsipeptide skeleton, β-hydroxytyrosine, and a highly modified side chain, which were not produced in a monoculture of the rare actinomycete Amycolatopsis sp. 26-4, were discovered in broth of its combined-culture with Tsukamurella pulmonis TP-B0596. The planar structures were elucidated by spectroscopic analyses (extensive 2D-NMR and MALDI-TOF MS/MS). The absolute configurations of component amino acids were unambiguously determined by the highly sensitive advanced Marfey's method we recently developed. Additionally, the structures of unstable/unusual moieties were corroborated by chemical synthesis and CD analysis.
-
enhancement of saccharothriolide production and discovery of a new metabolite saccharothriolide c2 by combined culture of saccharothrix sp and Tsukamurella pulmonis
Tetrahedron Letters, 2019Co-Authors: Yulu Jiang, Hiroyasu Onaka, Go Hirai, Taira Kato, Hideaki KakeyaAbstract:Abstract A combined-culture method was applied to saccharothriolide-producing strain Saccharothrix sp. A1506 with the mycolic acid-containing bacterium (MACB) Tsukamurella pulmonis TP-B0596. The combined-culture not only enhanced saccharothriolide production, but also produced saccharothriolide C2, a new C-2 epimer of saccharothriolide C, thereby indicating its potential for increasing the chemical diversity of microbial metabolites.
-
mirilactams c e novel polycyclic macrolactams isolated from combined culture of actinosynnema mirum nbrc 14064 and mycolic acid containing bacterium
Chemical & Pharmaceutical Bulletin, 2018Co-Authors: Shotaro Hoshino, Takayoshi Awakawa, Huiping Zhang, Fumiaki Hayashi, Masahiro Ozeki, Chin Piow Wong, Hiroyuki Morita, Hiroyasu OnakaAbstract:: Mycolic acid-containing bacteria (MACB) are known to activate cryptic natural product biosynthesis in co-cultures with actinobacteria. We cultured Actinosynnema mirum NBRC 14064, a producer of the mono-cyclic polyene macrolactam mirilactam A (6), with the MACB Tsukamurella pulmonis TP-B0596. As a result, three novel compounds (mirilactams C-E, 1-3) were produced in the co-culture conditions. Compounds 1-3 were likely derived from 6 by epoxidation and subsequent spontaneous cyclization. The chemical structures and stereochemistries of 1-3 were determined by spectroscopic analyses (NMR and MS), conformational searches in the optimized potentials for liquid simulations-3 (OPLS3) force field, and calculations of electronic circular dichroism (ECD).
Patrick C Y Woo - One of the best experts on this subject based on the ideXlab platform.
-
Tsukamurella ocularis sp nov and Tsukamurella hominis sp nov isolated from patients with conjunctivitis in hong kong
International Journal of Systematic and Evolutionary Microbiology, 2018Co-Authors: Jade L. L. Teng, Antonio H. Y. Ngan, Ying Tang, Samson S Y Wong, Susanna K P Lau, Tsz Ho Chiu, Zhe Zhao, Elaine Chan, Patrick C Y WooAbstract:Three bacterial strains, HKU63T, HKU64 and HKU65T, were isolated from the conjunctival swabs of three patients with conjunctivitis in Hong Kong. The three strains were aerobic, Gram-stain-positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from closely related Tsukamurella species. 16S rRNA gene sequence analysis revealed that the three strains shared identical sequences with each other, being most closely related to Tsukamurella tyrosinosolvens and Tsukamurella pulmonis, sharing 99.9 % sequence identity. Sequence analysis of three additional housekeeping genes, groEL, secA and rpoB, revealed 100 % nucleotide sequence identity between HKU63T and HKU64, 94.2–97.0 % nucleotide sequence identities between HKU63T/HKU64 and HKU65T and the three strains shared 82.9–98.9 % sequence identities with other currently recognized Tsukamurella species. DNA–DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (23.0±4.2 to 50.7±3.7 % DNA–DNA relatedness), of which HKU63T and HKU64 represented the same species (≥95.2±4.8 % DNA–DNA relatedness) while HKU65T represented another species. Fatty acid, mycolic acid, cell-wall sugar and peptidoglycan analyses showed that they were typical of members of Tsukamurella . The G+C content of strains HKU63T, HKU64 and HKU65T were 71.3±1.9, 71.3±2.0 and 71.2±2.3 mol% (mean±sd; n=3), respectively. A novel species, Tsukamurella ocularis sp. nov. is proposed to accommodate strains HKU63T and HKU64, with HKU63T (=JCM 31969T=DSM 105034T) designated as the type strain whilst another novel species, Tsukamurella hominis sp. nov., is proposed to accommodate the third strain, HKU65T, which is designated as the type strain (=JCM 31971T=DSM 105036T).
-
Tsukamurella serpentis sp nov isolated from the oral cavity of chinese cobras naja atra
International Journal of Systematic and Evolutionary Microbiology, 2016Co-Authors: Ying Tang, Antonio H. Y. Ngan, Jade L. L. Teng, Candy L W Cheung, Yi Huang, Samson S Y Wong, Eric K T Yip, Taklun Que, Susanna K P Lau, Patrick C Y WooAbstract:Two bacterial strains, HKU54T and HKU55, were isolated from the oral cavity of two Chinese cobras (Naja atra) in Hong Kong. 16S rRNA gene sequence analysis revealed 100 % sequence identity between HKU54T and HKU55, and the two strains shared 99.0 % sequence identities with T sukamurella inchonensis ATCC 700082T. The two strains had unique biochemical profiles distinguishable from closely related species of the genus Tsukamurella . DNA–DNA hybridization confirmed that they belonged to the same species (≥92.1±7.9 % DNA–DNA relatedness) but were distinct from all other known species of the genus Tsukamurella (≤52.6±5.3 % DNA–DNA relatedness). Chemotaxonomic and morphological analyses of the two strains also demonstrated results consistent with their classification in the genus Tsukamurella . The DNA G+C contents of strains HKU54T and HKU55 were 69.2±1.5 mol% and 69.2±1.3 mol% (mean±sd; n=3) respectively. A novel species, Tsukamurella serpentis sp. nov., is proposed to accommodate strains HKU54T and HKU55, with HKU54T (=JCM 31017T=DSM 100915T) designated as the type strain.
-
phylogenomic analyses and reclassification of species within the genus Tsukamurella insights to species definition in the post genomic era
Frontiers in Microbiology, 2016Co-Authors: Jade L. L. Teng, Ying Tang, Yi Huang, Samson S Y Wong, Susanna K P Lau, Fengbiao Guo, Wen Wei, Jonathan H K Chen, Patrick C Y WooAbstract:Owing to the highly similar phenotypic profiles, protein spectra and 16S rRNA gene sequences observed between three pairs of Tsukamurella species (Tsukamurella pulmonis/Tsukamurella spongiae, Tsukamurella tyrosinosolvens/Tsukamurella carboxy-divorans, and Tsukamurella pseudospumae/Tsukamurella sunchonensis), we hypothesize that and the six Tsukamurella species may have been misclassified and that there may only be three Tsukamurella species. In this study, we characterized the type strains of these six Tsukamurella species by tradition DNA-DNA hybridization (DDH) and "digital DDH" after genome sequencing to determine their exact taxonomic positions. Traditional DDH showed 81.2 ± 0.6% to 99.7 ± 1.0% DNA-DNA relatedness between the two Tsukamurella species in each of the three pairs, which was above the threshold for same species designation. "Digital DDH" based on Genome-To-Genome Distance Calculator and Average Nucleotide Identity for the three pairs also showed similarity results in the range of 82.3-92.9 and 98.1-99.1%, respectively, in line with results of traditional DDH. Based on these evidence and according to Rules 23a and 42 of the Bacteriological Code, we propose that T. spongiae Olson et al. 2007, should be reclassified as a later heterotypic synonym of T. pulmonis Yassin et al. 1996, T. carboxydivorans Park et al. 2009, as a later heterotypic synonym of T. tyrosinosolvens Yassin et al. 1997, and T. sunchonensis Seong et al. 2008 as a later heterotypic synonym of T. pseudospumae Nam et al. 2004. With the advancement of genome sequencing technologies, classification of bacterial species can be readily achieved by "digital DDH" than traditional DDH.
-
Tsukamurella hongkongensis sp nov and Tsukamurella sinensis sp nov isolated from patients with keratitis catheter related bacteraemia and conjunctivitis
International Journal of Systematic and Evolutionary Microbiology, 2016Co-Authors: Jade L. L. Teng, Antonio H. Y. Ngan, Ying Tang, Yi Huang, Samson S Y Wong, Susanna K P Lau, Chiching Tsang, Garnet K Y Choi, Patrick C Y WooAbstract:Three bacterial strains, HKU51T, HKU52T and HKU53, were isolated from a conjunctival swab, corneal scraping and blood culture of three patients in Hong Kong with conjunctivitis, keratitis and catheter-related bacteraemia, respectively. Cells were Gram-stain-positive, aerobic, catalase-positive, non-sporulating and non-motile bacilli. The three strains had unique biochemical profiles that were distinguishable from those of closely related species of the genus Tsukamurella. Fatty acids, mycolic acids, cell-wall sugars and peptidoglycan analyses showed that they were typical of members of Tsukamurella. 16S rRNA gene sequence analysis revealed 100 % sequence identity between HKU52T and HKU53, and the two strains shared 99.5 % sequence identity with Tsukamurella sunchonensis JCM 15929T and Tsukamurella pseudospumae JCM 13375T; HKU51T shared 99.6 % sequence identity with Tsukamurella pulmonis CCUG 35732T. The DNA G+C contents of strains HKU51T, HKU52T and HKU53 were 70.9 ± 2.2, 71.3 ± 2.1 and 71.2 ± 2.3 mol% (mean ± sd; n = 3), respectively. DNA–DNA hybridization confirmed that the novel strains were distinct from other known species of the genus Tsukamurella ( ≤ 50.1 ± 3.7 % DNA–DNA relatedness); two of the isolates, HKU52T and HKU53, represented the same species ( ≥ 94.6 ± 5.6 % DNA–DNA relatedness), while the third isolate, HKU51T, represented another species. The novel species Tsukamurella hongkongensis sp. nov. is proposed to accommodate strains HKU52T and HKU53, with HKU52T ( = JCM 30715T = DSM 100208T) as the type strain; whilst another novel species, Tsukamurella sinensis sp. nov., is proposed to accommodate the third isolate, HKU51T ( = JCM 30714T = DSM 100207T), which is designated the type strain.
-
characterization of a Tsukamurella pseudo outbreak by phenotypic tests 16s rrna sequencing pulsed field gel electrophoresis and metabolic footprinting
Journal of Clinical Microbiology, 2013Co-Authors: Ami M Y Fung, Jade L. L. Teng, Kwok-yung Yuen, Susanna K P Lau, Ching-wan Lam, Shirly O T Curreem, Kimchung Lee, Patrick C Y WooAbstract:We report a pseudo-outbreak of Tsukamurella due to improperly wrapped scissors used for processing of tissue specimens. A polyphasic approach, involving biochemical, genetic, and metabolomic techniques, was used in the laboratory investigation. This report highlights that early recognition of pseudo-outbreaks is important in preventing unnecessary and incorrect treatment of patients.
Jade L. L. Teng - One of the best experts on this subject based on the ideXlab platform.
-
Tsukamurella asaccharolytica sp nov Tsukamurella conjunctivitidis sp nov and Tsukamurella sputi sp nov isolated from patients with bacteraemia conjunctivitis and respiratory infection in hong kong
International Journal of Systematic and Evolutionary Microbiology, 2020Co-Authors: Antonio H. Y. Ngan, Jade L. L. Teng, Ying Tang, Samson S Y Wong, Tsz Ho Chiu, Jordan Y H Fong, Kenny M N Fok, Hwei Huih LeeAbstract:Three bacterial strains, HKU70T, HKU71T and HKU72T, were isolated from the conjunctival swab, blood and sputum samples of three patients with conjunctivitis, bacteraemia and respiratory infection, respectively, in Hong Kong. The three strains were aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from currently recognized Tsukamurella species. 16S rRNA, secA, rpoB and groEL gene sequence analyses revealed that the three strains shared 99.6-99.9, 94.5-96.8, 95.7-97.8 and 97.7-98.9 % nucleotide identities with their corresponding closest Tsukamurella species respectively. DNA–DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA–DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2–40.9 % Genome-to-Genome Distance Calculator and 86.3–88.9 % average nucleotide identity values]. Fatty acids, mycolic acids, cell-wall sugars and peptidoglycan analyses showed that they were typical of members of Tsukamurella . The G+C content determined based on the genome sequence of strains HKU70T, HKU71T and HKU72T were 69.9, 70.2 and 70.5 mol%, respectively. Taken together, our results supported the proposition and description of three new species, i.e. Tsukamurella sputi HKU70T (=JCM 33387T=DSM 109106T) sp. nov., Tsukamurella asaccharolytica HKU71T (=JCM 33388T=DSM 109107T) sp. nov. and Tsukamurella conjunctivitidis HKU72T (=JCM 33389T=DSM 109108T) sp. nov.
-
maldi tof ms for identification of Tsukamurella species Tsukamurella tyrosinosolvens as the predominant species associated with ocular infections
Emerging microbes & infections, 2018Co-Authors: Jade L. L. Teng, Antonio H. Y. Ngan, Ying Tang, Samson S Y Wong, Jonathan H K Chen, Zhe Zhao, Jordan Y H Fong, C P Wong, Kitty S C Fung, Taklun QueAbstract:Although Tsukamurella infections have been increasingly reported in Europe, Asia, America, and Africa, indicating that diseases caused by this group of bacteria are emerging in a global scale, species identification within this genus is difficult in most clinical microbiology laboratories. Recently, we showed that groEL gene sequencing is useful for identification of all existing Tsukamurella species. Nevertheless, PCR sequencing is still considered expensive, time-consuming, and technically demanding, and therefore is yet to be incorporated as a routine identification method in clinical laboratories. Using groEL gene sequencing as the reference method, 60 Tsukamurella isolates were identified as five different Tsukamurella species [T. tyrosinosolvens (n = 31), T. pulmonis (n = 25), T. hongkongensis (n = 2), T. strandjordii (n = 1), and T. sinensis (n = 1)]. The most common source of the patient isolates were the eye (n = 18), sputum (n = 6), and blood (n = 6). None of the 60 isolates were identified correctly to species level by MALDI-TOF MS with the original Bruker database V.6.0.0.0. Using the Bruker database extended with 15 type and reference strains which covered all the currently recognized 11 Tsukamurella species, 59 of the 60 isolates were correctly identified to the species level with score ≥2.0. MALDI-TOF MS should be useful for routine species identification of Tsukamurella in clinical microbiology laboratories after optimization of the database. T. tyrosinosolvens was the most common species observed in patients with Tsukamurella infections and the predominant species associated with ocular infections.
-
Tsukamurella ocularis sp nov and Tsukamurella hominis sp nov isolated from patients with conjunctivitis in hong kong
International Journal of Systematic and Evolutionary Microbiology, 2018Co-Authors: Jade L. L. Teng, Antonio H. Y. Ngan, Ying Tang, Samson S Y Wong, Susanna K P Lau, Tsz Ho Chiu, Zhe Zhao, Elaine Chan, Patrick C Y WooAbstract:Three bacterial strains, HKU63T, HKU64 and HKU65T, were isolated from the conjunctival swabs of three patients with conjunctivitis in Hong Kong. The three strains were aerobic, Gram-stain-positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from closely related Tsukamurella species. 16S rRNA gene sequence analysis revealed that the three strains shared identical sequences with each other, being most closely related to Tsukamurella tyrosinosolvens and Tsukamurella pulmonis, sharing 99.9 % sequence identity. Sequence analysis of three additional housekeeping genes, groEL, secA and rpoB, revealed 100 % nucleotide sequence identity between HKU63T and HKU64, 94.2–97.0 % nucleotide sequence identities between HKU63T/HKU64 and HKU65T and the three strains shared 82.9–98.9 % sequence identities with other currently recognized Tsukamurella species. DNA–DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (23.0±4.2 to 50.7±3.7 % DNA–DNA relatedness), of which HKU63T and HKU64 represented the same species (≥95.2±4.8 % DNA–DNA relatedness) while HKU65T represented another species. Fatty acid, mycolic acid, cell-wall sugar and peptidoglycan analyses showed that they were typical of members of Tsukamurella . The G+C content of strains HKU63T, HKU64 and HKU65T were 71.3±1.9, 71.3±2.0 and 71.2±2.3 mol% (mean±sd; n=3), respectively. A novel species, Tsukamurella ocularis sp. nov. is proposed to accommodate strains HKU63T and HKU64, with HKU63T (=JCM 31969T=DSM 105034T) designated as the type strain whilst another novel species, Tsukamurella hominis sp. nov., is proposed to accommodate the third strain, HKU65T, which is designated as the type strain (=JCM 31971T=DSM 105036T).
-
the groel gene is a promising target for species level identification of Tsukamurella
Journal of Clinical Microbiology, 2017Co-Authors: Jade L. L. Teng, Antonio H. Y. Ngan, Ying Tang, Candy L W Cheung, Tsz Ho Chiu, Cheung Ngai, Samson S Y WongAbstract:Differentiation of Tsukamurella from the other related genera, such as Nocardia, Rhodococcus, and Gordonia, and species-level identification within these genera have been difficult in most clinical microbiology laboratories, as they share similar phenotypic properties. Among various molecular
-
Tsukamurella serpentis sp nov isolated from the oral cavity of chinese cobras naja atra
International Journal of Systematic and Evolutionary Microbiology, 2016Co-Authors: Ying Tang, Antonio H. Y. Ngan, Jade L. L. Teng, Candy L W Cheung, Yi Huang, Samson S Y Wong, Eric K T Yip, Taklun Que, Susanna K P Lau, Patrick C Y WooAbstract:Two bacterial strains, HKU54T and HKU55, were isolated from the oral cavity of two Chinese cobras (Naja atra) in Hong Kong. 16S rRNA gene sequence analysis revealed 100 % sequence identity between HKU54T and HKU55, and the two strains shared 99.0 % sequence identities with T sukamurella inchonensis ATCC 700082T. The two strains had unique biochemical profiles distinguishable from closely related species of the genus Tsukamurella . DNA–DNA hybridization confirmed that they belonged to the same species (≥92.1±7.9 % DNA–DNA relatedness) but were distinct from all other known species of the genus Tsukamurella (≤52.6±5.3 % DNA–DNA relatedness). Chemotaxonomic and morphological analyses of the two strains also demonstrated results consistent with their classification in the genus Tsukamurella . The DNA G+C contents of strains HKU54T and HKU55 were 69.2±1.5 mol% and 69.2±1.3 mol% (mean±sd; n=3) respectively. A novel species, Tsukamurella serpentis sp. nov., is proposed to accommodate strains HKU54T and HKU55, with HKU54T (=JCM 31017T=DSM 100915T) designated as the type strain.
Susanna K P Lau - One of the best experts on this subject based on the ideXlab platform.
-
Tsukamurella ocularis sp nov and Tsukamurella hominis sp nov isolated from patients with conjunctivitis in hong kong
International Journal of Systematic and Evolutionary Microbiology, 2018Co-Authors: Jade L. L. Teng, Antonio H. Y. Ngan, Ying Tang, Samson S Y Wong, Susanna K P Lau, Tsz Ho Chiu, Zhe Zhao, Elaine Chan, Patrick C Y WooAbstract:Three bacterial strains, HKU63T, HKU64 and HKU65T, were isolated from the conjunctival swabs of three patients with conjunctivitis in Hong Kong. The three strains were aerobic, Gram-stain-positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from closely related Tsukamurella species. 16S rRNA gene sequence analysis revealed that the three strains shared identical sequences with each other, being most closely related to Tsukamurella tyrosinosolvens and Tsukamurella pulmonis, sharing 99.9 % sequence identity. Sequence analysis of three additional housekeeping genes, groEL, secA and rpoB, revealed 100 % nucleotide sequence identity between HKU63T and HKU64, 94.2–97.0 % nucleotide sequence identities between HKU63T/HKU64 and HKU65T and the three strains shared 82.9–98.9 % sequence identities with other currently recognized Tsukamurella species. DNA–DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (23.0±4.2 to 50.7±3.7 % DNA–DNA relatedness), of which HKU63T and HKU64 represented the same species (≥95.2±4.8 % DNA–DNA relatedness) while HKU65T represented another species. Fatty acid, mycolic acid, cell-wall sugar and peptidoglycan analyses showed that they were typical of members of Tsukamurella . The G+C content of strains HKU63T, HKU64 and HKU65T were 71.3±1.9, 71.3±2.0 and 71.2±2.3 mol% (mean±sd; n=3), respectively. A novel species, Tsukamurella ocularis sp. nov. is proposed to accommodate strains HKU63T and HKU64, with HKU63T (=JCM 31969T=DSM 105034T) designated as the type strain whilst another novel species, Tsukamurella hominis sp. nov., is proposed to accommodate the third strain, HKU65T, which is designated as the type strain (=JCM 31971T=DSM 105036T).
-
Tsukamurella serpentis sp nov isolated from the oral cavity of chinese cobras naja atra
International Journal of Systematic and Evolutionary Microbiology, 2016Co-Authors: Ying Tang, Antonio H. Y. Ngan, Jade L. L. Teng, Candy L W Cheung, Yi Huang, Samson S Y Wong, Eric K T Yip, Taklun Que, Susanna K P Lau, Patrick C Y WooAbstract:Two bacterial strains, HKU54T and HKU55, were isolated from the oral cavity of two Chinese cobras (Naja atra) in Hong Kong. 16S rRNA gene sequence analysis revealed 100 % sequence identity between HKU54T and HKU55, and the two strains shared 99.0 % sequence identities with T sukamurella inchonensis ATCC 700082T. The two strains had unique biochemical profiles distinguishable from closely related species of the genus Tsukamurella . DNA–DNA hybridization confirmed that they belonged to the same species (≥92.1±7.9 % DNA–DNA relatedness) but were distinct from all other known species of the genus Tsukamurella (≤52.6±5.3 % DNA–DNA relatedness). Chemotaxonomic and morphological analyses of the two strains also demonstrated results consistent with their classification in the genus Tsukamurella . The DNA G+C contents of strains HKU54T and HKU55 were 69.2±1.5 mol% and 69.2±1.3 mol% (mean±sd; n=3) respectively. A novel species, Tsukamurella serpentis sp. nov., is proposed to accommodate strains HKU54T and HKU55, with HKU54T (=JCM 31017T=DSM 100915T) designated as the type strain.
-
phylogenomic analyses and reclassification of species within the genus Tsukamurella insights to species definition in the post genomic era
Frontiers in Microbiology, 2016Co-Authors: Jade L. L. Teng, Ying Tang, Yi Huang, Samson S Y Wong, Susanna K P Lau, Fengbiao Guo, Wen Wei, Jonathan H K Chen, Patrick C Y WooAbstract:Owing to the highly similar phenotypic profiles, protein spectra and 16S rRNA gene sequences observed between three pairs of Tsukamurella species (Tsukamurella pulmonis/Tsukamurella spongiae, Tsukamurella tyrosinosolvens/Tsukamurella carboxy-divorans, and Tsukamurella pseudospumae/Tsukamurella sunchonensis), we hypothesize that and the six Tsukamurella species may have been misclassified and that there may only be three Tsukamurella species. In this study, we characterized the type strains of these six Tsukamurella species by tradition DNA-DNA hybridization (DDH) and "digital DDH" after genome sequencing to determine their exact taxonomic positions. Traditional DDH showed 81.2 ± 0.6% to 99.7 ± 1.0% DNA-DNA relatedness between the two Tsukamurella species in each of the three pairs, which was above the threshold for same species designation. "Digital DDH" based on Genome-To-Genome Distance Calculator and Average Nucleotide Identity for the three pairs also showed similarity results in the range of 82.3-92.9 and 98.1-99.1%, respectively, in line with results of traditional DDH. Based on these evidence and according to Rules 23a and 42 of the Bacteriological Code, we propose that T. spongiae Olson et al. 2007, should be reclassified as a later heterotypic synonym of T. pulmonis Yassin et al. 1996, T. carboxydivorans Park et al. 2009, as a later heterotypic synonym of T. tyrosinosolvens Yassin et al. 1997, and T. sunchonensis Seong et al. 2008 as a later heterotypic synonym of T. pseudospumae Nam et al. 2004. With the advancement of genome sequencing technologies, classification of bacterial species can be readily achieved by "digital DDH" than traditional DDH.
-
Tsukamurella hongkongensis sp nov and Tsukamurella sinensis sp nov isolated from patients with keratitis catheter related bacteraemia and conjunctivitis
International Journal of Systematic and Evolutionary Microbiology, 2016Co-Authors: Jade L. L. Teng, Antonio H. Y. Ngan, Ying Tang, Yi Huang, Samson S Y Wong, Susanna K P Lau, Chiching Tsang, Garnet K Y Choi, Patrick C Y WooAbstract:Three bacterial strains, HKU51T, HKU52T and HKU53, were isolated from a conjunctival swab, corneal scraping and blood culture of three patients in Hong Kong with conjunctivitis, keratitis and catheter-related bacteraemia, respectively. Cells were Gram-stain-positive, aerobic, catalase-positive, non-sporulating and non-motile bacilli. The three strains had unique biochemical profiles that were distinguishable from those of closely related species of the genus Tsukamurella. Fatty acids, mycolic acids, cell-wall sugars and peptidoglycan analyses showed that they were typical of members of Tsukamurella. 16S rRNA gene sequence analysis revealed 100 % sequence identity between HKU52T and HKU53, and the two strains shared 99.5 % sequence identity with Tsukamurella sunchonensis JCM 15929T and Tsukamurella pseudospumae JCM 13375T; HKU51T shared 99.6 % sequence identity with Tsukamurella pulmonis CCUG 35732T. The DNA G+C contents of strains HKU51T, HKU52T and HKU53 were 70.9 ± 2.2, 71.3 ± 2.1 and 71.2 ± 2.3 mol% (mean ± sd; n = 3), respectively. DNA–DNA hybridization confirmed that the novel strains were distinct from other known species of the genus Tsukamurella ( ≤ 50.1 ± 3.7 % DNA–DNA relatedness); two of the isolates, HKU52T and HKU53, represented the same species ( ≥ 94.6 ± 5.6 % DNA–DNA relatedness), while the third isolate, HKU51T, represented another species. The novel species Tsukamurella hongkongensis sp. nov. is proposed to accommodate strains HKU52T and HKU53, with HKU52T ( = JCM 30715T = DSM 100208T) as the type strain; whilst another novel species, Tsukamurella sinensis sp. nov., is proposed to accommodate the third isolate, HKU51T ( = JCM 30714T = DSM 100207T), which is designated the type strain.
-
characterization of a Tsukamurella pseudo outbreak by phenotypic tests 16s rrna sequencing pulsed field gel electrophoresis and metabolic footprinting
Journal of Clinical Microbiology, 2013Co-Authors: Ami M Y Fung, Jade L. L. Teng, Kwok-yung Yuen, Susanna K P Lau, Ching-wan Lam, Shirly O T Curreem, Kimchung Lee, Patrick C Y WooAbstract:We report a pseudo-outbreak of Tsukamurella due to improperly wrapped scissors used for processing of tissue specimens. A polyphasic approach, involving biochemical, genetic, and metabolomic techniques, was used in the laboratory investigation. This report highlights that early recognition of pseudo-outbreaks is important in preventing unnecessary and incorrect treatment of patients.
