The Experts below are selected from a list of 249 Experts worldwide ranked by ideXlab platform
Hans-peter Klenk - One of the best experts on this subject based on the ideXlab platform.
-
High quality draft genome sequence of Corynebacterium ulceribovis type strain IMMIB-L1395^T (DSM 45146^T)
Standards in Genomic Sciences, 2015Co-Authors: Atteyet F. Yassin, Alla Lapidus, James Han, T.b.k. Reddy, Marcel Huntemann, Amrita Pati, Natalia Ivanova, Victor Markowitz, Tanja Woyke, Hans-peter KlenkAbstract:Corynebacterium ulceribovis strain IMMIB L-1395^T (= DSM 45146^T) is an aerobic to facultative anaerobic, Gram-positive, non-spore-forming, non-motile rod-shaped bacterium that was isolated from the skin of the udder of a cow, in Schleswig Holstein, Germany. The cell wall of C. ulceribovis contains corynemycolic Acids. The cellular fatty Acids are those described for the genus Corynebacterium , but Tuberculostearic Acid is not present. Here we describe the features of C. ulceribovis strain IMMIB L-1395^T, together with genome sequence information and its annotation. The 2,300,451 bp long genome containing 2,104 protein-coding genes and 54 RNA-encoding genes and is part of the Genomic Encyclopedia of Type Strains, Phase I: the one thousand microbial genomes (KMG) project.
-
High quality draft genome sequence of Corynebacterium ulceribovis type strain IMMIB-L1395(T) (DSM 45146(T)).
Standards in genomic sciences, 2015Co-Authors: A F Yassin, Alla Lapidus, James Han, T.b.k. Reddy, Marcel Huntemann, Amrita Pati, Natalia Ivanova, Victor Markowitz, Tanja Woyke, Hans-peter KlenkAbstract:Corynebacterium ulceribovis strain IMMIB L-1395(T) (= DSM 45146(T)) is an aerobic to facultative anaerobic, Gram-positive, non-spore-forming, non-motile rod-shaped bacterium that was isolated from the skin of the udder of a cow, in Schleswig Holstein, Germany. The cell wall of C. ulceribovis contains corynemycolic Acids. The cellular fatty Acids are those described for the genus Corynebacterium, but Tuberculostearic Acid is not present. Here we describe the features of C. ulceribovis strain IMMIB L-1395(T), together with genome sequence information and its annotation. The 2,300,451 bp long genome containing 2,104 protein-coding genes and 54 RNA-encoding genes and is part of the Genomic Encyclopedia of Type Strains, Phase I: the one thousand microbial genomes (KMG) project.
G L French - One of the best experts on this subject based on the ideXlab platform.
-
A Tuberculostearic Acid Assay in the Diagnosis of Sputum Smear-Negative Pulmonary Tuberculosis
Annals of Internal Medicine, 2020Co-Authors: Joseph Pang, Hok Sum Chan, Chiu Yeung Chan, Siu Wai Cheung, G L FrenchAbstract:Abstract Objective:To determine whether the detection of Tuberculostearic Acid (TBSA) in bronchial aspirate and bronchoalveolar lavage specimens is useful for the rapid diagnosis of active pulmonar...
-
Diagnosis of tuberculous pleural effusion by the detection of Tuberculostearic Acid in pleural aspirates.
Chest, 1991Co-Authors: Wai Yew, Siu Wai Cheung, Chiu Yeung Chan, Susan Yuk-lin Kwan, G L FrenchAbstract:Detection of TBSA was attempted in pleural aspirates of 74 patients with tuberculous and 44 patients with nontuberculous pleural effusion by gas chromatography/mass spectrometry with selected ion monitoring. The results were disappointing with a test sensitivity of 67.6 percent and a specificity of 52.3 percent. In contrast, histologic examination of pleural biopsies gave a diagnostic sensitivity of 71.0 percent. Pleural biopsy remains a better investigational procedure for the diagnosis of tuberculous pleural effusion.
A F Yassin - One of the best experts on this subject based on the ideXlab platform.
-
High quality draft genome sequence of Corynebacterium ulceribovis type strain IMMIB-L1395(T) (DSM 45146(T)).
Standards in genomic sciences, 2015Co-Authors: A F Yassin, Alla Lapidus, James Han, T.b.k. Reddy, Marcel Huntemann, Amrita Pati, Natalia Ivanova, Victor Markowitz, Tanja Woyke, Hans-peter KlenkAbstract:Corynebacterium ulceribovis strain IMMIB L-1395(T) (= DSM 45146(T)) is an aerobic to facultative anaerobic, Gram-positive, non-spore-forming, non-motile rod-shaped bacterium that was isolated from the skin of the udder of a cow, in Schleswig Holstein, Germany. The cell wall of C. ulceribovis contains corynemycolic Acids. The cellular fatty Acids are those described for the genus Corynebacterium, but Tuberculostearic Acid is not present. Here we describe the features of C. ulceribovis strain IMMIB L-1395(T), together with genome sequence information and its annotation. The 2,300,451 bp long genome containing 2,104 protein-coding genes and 54 RNA-encoding genes and is part of the Genomic Encyclopedia of Type Strains, Phase I: the one thousand microbial genomes (KMG) project.
-
Rapid Chemotaxonomic Diagnosis of Human Tuberculosis
Zentralblatt fur Bakteriologie : international journal of medical microbiology, 1996Co-Authors: A F Yassin, H Brzezinka, Ernst Molitor, K. P. SchaalAbstract:65 different clinical specimens from patients suspected of being infected with Mycobacterium tuberculosis were examined by three different diagnostic methods. Two of these methods were the conventional microscopic and cultural examinations. The third, a modern chemotaxonomical method is based upon the detection of Tuberculostearic Acid by GC-MS analysis using selected ion monitoring (GC-MS/SIM). Comparison of the results of the GC-MS analysis with those of the conventional methods has indicated that Tuberculostearic Acid analysis can be used for diagnosing tuberculosis under diagnostic routine conditions. The GC-MS method is rapid, usually providing results within 20 hours or less.
-
lentzea gen nov a new genus of the order actinomycetales
International Journal of Systematic and Evolutionary Microbiology, 1995Co-Authors: A F Yassin, H Brzezinka, K D Jahnke, H Weissbrodt, Herbert Budzikiewicz, Erko Stackebrandt, Fred A. Rainey, K. P. SchaalAbstract:We describe a new genus of mesophilic actinomycetes, for which we propose the name Lentzea. The strains of this genus form abundant aerial hyphae that fragment into rod-shaped elements. Whole-cell hydrolysates contain the meso isomer of diaminopimelic Acid and no characteristic sugar (wall chemotype III). The phospholipid pattern type is type PII (phosphatidylethanolamine is the characteristic phospholipid); the major menaquinone is MK-9. The fatty Acid profile comprises saturated, unsaturated, and branched-chain fatty Acids of the iso and anteiso types in addition to Tuberculostearic Acid (10Me-C18:0). A 16S ribosomal DNA sequence analysis revealed that the genus Lentzea is phylogenically related to the genera Actinosynnema, Saccharothrix, and Kutzneria. The type species of this genus is Lentzea albidocapillata sp. nov.; the type strain of this species is strain IMMIB D-958 (= DSM 44073).
-
Cellular fatty Acid methyl ester profiles as a tool in the differentiation of members of the genus Mycobacterium
Zentralblatt fur Bakteriologie : international journal of medical microbiology, 1993Co-Authors: A F Yassin, H Brzezinka, K. P. SchaalAbstract:The cellular fatty Acid profiles of 84 strains belonging to 53 different species of the genus Mycobacterium were determined by gas liquid chromatography-mass spectrometry (GCMS). Two main types and four subtypes of fatty Acid profiles were recognizable. The first main type is the G type, nominally referring to M. gordonae, members of which lack Tuberculostearic Acid or other 10-methyl branched-chain fatty Acids, but contain normal saturated and unsaturated fatty Acids. This type is further subdivided into the G alpha subtype that is characterized by 2-methyl tetradecanoic Acid (2-Me-C14:0) as the only 2-methyl branched fatty Acid. Strains belonging to the second main type, the T type, nominally referring to tuberculosis, contain Tuberculostearic Acid and other 10-methyl branched Acids in addition to the normal saturated and unsaturated ones. This type has been further subdivided into three subtypes: the T alpha subtype that does not contain any 2-methyl branched fatty Acids; the T beta subtype that contains both 2-methyl tetradecanoic (2-Me-C14:0) and 2,4-dimethyl tetradecanoic (2,4-DMe-C14:0) Acids as 2-methyl branched fatty Acids; the T gamma subtype which contains 2-methyl dodecanoic (2-Me-C12:0), 2,4-dimethyl dodecanoic (2,4-DMe-C12:0) and 2,4-dimethyl tetradecanoic (2,4-DMe-C14:0) Acids as 2-methyl branched-chain Acids. Fatty Acid analysis showed a great homogeneity within the genus and the profiles produced were not very helpful in distinguishing between members of the genus Mycobacterium except for the identification of M. gordonae, M. kansasii, and M. gastri.
I. Nuzzo - One of the best experts on this subject based on the ideXlab platform.
-
apoptosis modulation by mycolic Acid Tuberculostearic Acid and trehalose 6 6 dimycolate
Journal of Infection, 2002Co-Authors: I. Nuzzo, Marilena Galdiero, C. Bentivoglio, R. Galdiero, Romano C CarratelliAbstract:Abstract The object of our study is to demonstrate that some components of M. tuberculosis , such as cord factor or mycolic Acid or whole bacteria can prolong cell survival compared to controls. The cells treated with cord factor or mycolic Acid at a concentration of 5 μg/ml were 65±8% viable reaching 70±8% at a concentration of 10 μg/ml. The cells treated with heat killed mycobacteria were 70±8% viable; while control cells exhibited a viability 50±7%. Conversely, Tuberculostearic Acid induced early cell death. The results also demonstrated a dose-dependent effect on the viability or induction of macrophage apoptosis. We also showed that prolonged viability of the treated cells with mycolic Acid or cord factor (+20±4% and +25±5%, respectively) was correlated with a significant increase in Bcl-2 expression. The treated cells with whole bacteria presented a Bcl-2 expression of 40±6%, while Fas expression was not changed compared to controls. This study confirm that at the site of mycobacterial infection, necrosis, apoptosis or prolonged survival of the cells depend on the quantity and quality of the molecules expressed by the mycobacteria; whether necrosis or apoptosis or prolonged survival is more or less favorable to the host likely depends on several factors regarding the inflammatory and immune response, both markedly stimulated by mycobacteria.
-
Apoptosis Modulation by Mycolic Acid, Tuberculostearic Acid and Trehalose 6,6′-Dimycolate
The Journal of infection, 2002Co-Authors: I. Nuzzo, Marilena Galdiero, C. Bentivoglio, R. Galdiero, C. Romano CarratelliAbstract:Abstract The object of our study is to demonstrate that some components of M. tuberculosis , such as cord factor or mycolic Acid or whole bacteria can prolong cell survival compared to controls. The cells treated with cord factor or mycolic Acid at a concentration of 5 μg/ml were 65±8% viable reaching 70±8% at a concentration of 10 μg/ml. The cells treated with heat killed mycobacteria were 70±8% viable; while control cells exhibited a viability 50±7%. Conversely, Tuberculostearic Acid induced early cell death. The results also demonstrated a dose-dependent effect on the viability or induction of macrophage apoptosis. We also showed that prolonged viability of the treated cells with mycolic Acid or cord factor (+20±4% and +25±5%, respectively) was correlated with a significant increase in Bcl-2 expression. The treated cells with whole bacteria presented a Bcl-2 expression of 40±6%, while Fas expression was not changed compared to controls. This study confirm that at the site of mycobacterial infection, necrosis, apoptosis or prolonged survival of the cells depend on the quantity and quality of the molecules expressed by the mycobacteria; whether necrosis or apoptosis or prolonged survival is more or less favorable to the host likely depends on several factors regarding the inflammatory and immune response, both markedly stimulated by mycobacteria.
Pat Sandra - One of the best experts on this subject based on the ideXlab platform.
-
A rapid method of diagnosing pulmonary tuberculosis using stir bar sorptive extraction-thermal desorption-gas chromatography-mass spectrometry.
Journal of chromatography. A, 2005Co-Authors: Adriana Stopforth, Andreas Tredoux, Andrew Crouch, Paul Van Helden, Pat SandraAbstract:A fast method for detection of Tuberculostearic Acid (TBSA) in sputum samples is described. The samples, obtained from patients with known or suspected pulmonary tuberculosis, were decontaminated and concentrated before being analyzed by stir bar sorptive extraction-thermal desorption-gas chromatography-mass spectrometry (SBSE-TD-GC-MS). Prior to extraction, the mycobacterial lipids were hydrolyzed and then derivatized with ethyl chloroformate to increase the sorption of the compounds by the polydimethylsiloxane (PDMS) stir bar coating. The limit of detection (LOD) is 0.2 ng ml(-1). Four sputum samples that were classified by direct microscopy as smear-positive or negative were analyzed by GC-MS. TBSA was detected at concentrations ranging from 0.47 to 2.3 ng ml(-1). The method is sufficiently sensitive to detect TBSA directly in clinical samples without the need to culture the organisms.
