The Experts below are selected from a list of 192 Experts worldwide ranked by ideXlab platform
D. M. Sylvia - One of the best experts on this subject based on the ideXlab platform.
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Comparisons of vesicular-arbuscular mycorrhizal species and inocula formulations in a commercial nursery and on diverse Florida beaches
Biology and Fertility of Soils, 1993Co-Authors: D. M. Sylvia, A. G. Jarstfer, M. VosátkaAbstract:For efficient use of mycorrhizal inoculum the effectiveness of the isolate used and the rate of application required for maximum colonization must be known. The objectives of this research were to (1) define the lower limit of inoculum density required for maximum colonization of Uniola paniculata in a commercial nursery and (2) evaluate the performance of a selected native dune vesicular-arbuscular mycorrhizal (VAM) isolate versus a commercially available non-dune VAM (foreign) isolate on three diverse Florida beaches. An inoculum-dilution study was conducted in a commercial nursery with cutroot inoculum of a Glomus sp. that had been isolated from a Florida dune. Maximum colonization was achieved with approximately 1 propagule ml^-1 of growth medium. In a separate nursery study, 10 inoculation treatments (combinations of inoculum source and level) were established in the commercial nursery. Treatments included cut-root and sheared-root inoculum of the native dune isolate, and Nutri-Link, a commercial inoculum of G. intraradices . Colonized plants from selected treatments were transplanted to beach sites around Florida. At Miami Beach, after one growing season, the shoot mass of plants inoculated with the native isolate was approximately twice that of plants inoculated with the foreign isolate. At Katherine Abbey Hanna Park and Eglin Air Force Base there were no significant inoculum source effects on shoot mass or root length after one growing season. However, the native isolate produced a greater colonized root length than the foreign isolate in all plantings. The soil hyphal density was measured at Eglin Air Force Base, and the results showed that plants inoculated with the native isolate had more soil hyphae (4.33 mg^-1) than plants inoculated with the foreign isolate (3.65 mg^-1) or the non-inoculated plants (2.12 mg^-1). Even where there were no obvious shoot growth responses, mycorrhizal inoculation may have an important effect on dune stabilization, as soil hyphae are known to bind sand grains and improve dune stability.
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Interaction of rhizosphere bacteria, fertilizer, and vesicular-arbuscular mycorrhizal fungi with sea oats.
Applied and Environmental Microbiology, 1990Co-Authors: M. E. Will, D. M. SylviaAbstract:Plants must be established quickly on replenished beaches in order to stabilize the sand and begin the dune-building process. The objective of this research was to determine whether inoculation of sea oats (Uniola paniculata L.) with bacteria (indigenous rhizosphere bacteria and N2 fixers) alone or in combination with vesicular-arbuscular mycorrhizal fungi would enhance plant growth in beach sand. At two fertilizer-N levels, Klebsiella pneumoniae and two Azospirillum spp. did not provide the plants with fixed atmospheric N; however, K. pneumoniae increased root and shoot growth. When a sparingly soluble P source (CaHPO4) was added to two sands, K. pneumoniae increased plant growth in sand with a high P content. The phosphorus content of shoots was not affected by bacterial inoculation, indicating that a mechanism other than bacterially enhanced P availability to plants was responsible for the growth increases. When sea oats were inoculated with either K. pneumoniae or Acaligenes denitrificans and a mixed Glomus inoculum, there was no consistent evidence of a synergistic effect on plant growth. Nonetheless, bacterial inoculation increased root colonization by vesicular-arbuscular mycorrhizal fungi when the fungal inoculum consisted of colonized roots but had no effect on colonization when the inoculum consisted of spores alone. K. pneumoniae was found to increase spore germination and hyphal growth of Glomus deserticola compared with the control. The use of bacterial inoculants to enhance establishment of pioneer dune plants warrants further study.
Lisa A. Donovan - One of the best experts on this subject based on the ideXlab platform.
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Inter-island but not intra-island divergence among populations of sea oats, Uniola paniculata L. (Poaceae)
Conservation Genetics, 2013Co-Authors: Cara L. Gormally, J. L. Hamrick, Lisa A. DonovanAbstract:Understanding the underlying causes of phenotypic trait variation among populations is important for informing conservation decisions. This knowledge can be used to determine whether locality matters when sourcing populations for habitat restoration. Uniola paniculata is a federally protected coastal dune grass native to the southeastern Atlantic and the Gulf coasts of the USA that is often used to stabilize restored dune habitats. This study uses neutral genetic markers (allozymes) and a greenhouse common garden study to determine the relative contributions of neutral evolutionary processes and natural selection to patterns of phenotypic variation among natural populations of U. paniculata . Seeds were sourced from foredune and backdune populations spanning shoreline-to-landward environmental gradients on each of four Georgia barrier islands. Based on previous work, we expected to find evidence of divergent selection among populations located on the shoreline-to-landward environmental gradient. However, differences among islands, rather than intra-island habitat differences, drive divergent selection on aboveground and total biomass. The lack of evidence for divergent selection across the shoreline-to-landward gradient suggests that previously documented intra-island trait variation is likely due to phenotypic plasticity. Our findings have implications for conservation and restoration efforts involving U. paniculata , as there is evidence for divergent selection among populations located on neighboring islands.
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No Evidence of Local Adaptation in Uniola paniculata L. (Poaceae), a Coastal Dune Grass
Southeastern Naturalist, 2011Co-Authors: Cara Gormally, Lisa A. DonovanAbstract:Abstract Studies of local adaptation generally investigate plants growing in relatively stable habitats. We asked whether populations of the long-lived clonal grass Uniola paniculata (Sea Oats) are locally adapted to microhabitats in the southeastern US coastal dunes, a habitat characterized by dynamic environmental gradients spanning relatively small distances. Although vegetative zonation is well characterized across these gradients, little is known about intraspecific evolutionary responses of species spanning the gradients. Plants from the foredune and backdune areas of the gradient (
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no evidence of local adaptation in Uniola paniculata l poaceae a coastal dune grass
Southeastern Naturalist, 2011Co-Authors: Cara Gormally, Lisa A. DonovanAbstract:Abstract Studies of local adaptation generally investigate plants growing in relatively stable habitats. We asked whether populations of the long-lived clonal grass Uniola paniculata (Sea Oats) are locally adapted to microhabitats in the southeastern US coastal dunes, a habitat characterized by dynamic environmental gradients spanning relatively small distances. Although vegetative zonation is well characterized across these gradients, little is known about intraspecific evolutionary responses of species spanning the gradients. Plants from the foredune and backdune areas of the gradient (<10 m and 40–60 m from the shoreline, respectively) were reciprocally transplanted into experimental plots in both habitats. Although foredune plots were washed away by storms before harvest, the foredune plants demonstrated no early advantage in stem diameter or height growth, and thus there was no support for local adaptation in foredune plants. In the backdune plots, the backdune plants demonstrated no early growth adv...
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Responses of Uniola paniculata L. (Poaceae), an Essential Dune-Building Grass, to Complex Changing Environmental Gradients on the Coastal Dunes
Estuaries and Coasts, 2010Co-Authors: Cara L. Gormally, Lisa A. DonovanAbstract:Coastal dunes are well known for plant species zonation but less is known about species-specific responses to underlying environmental gradients. We investigated variation in morphological traits and tissue nutrient concentration in Uniola paniculata , along a shoreline-to-landward gradient (transects spanning from the dunes directly behind the high tide mark to 40–100 m inland) in the southeast USA. Several environmental factors decreased with distance from the shoreline (soil B, K, Mg, Na; salinity, pH, and sand accretion), and differences were most pronounced between the 10 m closest to the shoreline and the remainder of the transect. In the 10 m closest to the shoreline, 94% more sand accumulated, which was 31% more saline. Additionally, plants here were taller, contained higher aboveground tissue N and K, and a higher percentage tended to flower. This contrasts with patterns found in salt marshes and saline desert dunes, where plant size is often negatively correlated with salinity. During the 2 years following the planned study, storms washed out ≤25 m of the transects. Resampling of the remaining sites demonstrated that even after erosion of the dune profile, a higher percentage of the plants in the 10 m closest to the shoreline plants tended to flower, relative to populations located further from the shore. Our findings suggested that the environment and plant response in the shoreward 10 m can re-establish relatively quickly.
David L. Nash - One of the best experts on this subject based on the ideXlab platform.
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Influence of Selected Surface Disinfestants, Fungicides, and Temperature on Seed Germination and Initial Growth of Southern Seaoats (Uniola paniculata)
Journal of environmental horticulture, 2017Co-Authors: Tyler L. Burgess, Frank A. Blazich, David L. Nash, Betsy Randall-schadelAbstract:Abstract Seeds of southern seaoats (Uniola paniculata L.) were removed from storage at 4C (39F) and treated with the following selected surface disinfestants, fungicides, or combinations of these c...
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nitrogen nutrition of southern seaoats Uniola paniculata grown in the float system
Journal of environmental horticulture, 2017Co-Authors: Daniel S Norden, Frank A. Blazich, Stuart L Warren, David L. NashAbstract:Abstract Seeds of southern seaoats (Uniola paniculata L.) were removed from storage in July 2004, surface disinfested with 2.6% sodium hypochlorite (NaOCl) for 15 min, and sown in styrofoam tobacco (Nicotiana tabacum L.) float trays (flats) filled with a vermiculite-based hydroponic substrate. Trays were floated in plastic tubs (one tray per tub) containing a complete nutrient solution with nitrogen (N) at 10, 60, 120, 180, or 240 mg·L−1 (ppm) from a 2N–3.5P–1K ratio (8N–32P2O5–5K2O) liquid slow-release fertilizer. After 10 weeks the study was terminated and data recorded. Total plant, top, leaf, stem, and root dry weights increased quadratically with increasing nitrogen application rate (NAR) with maximum dry weights calculated to occur with N at 140 to 150 mg-L−1, respectively. Other growth indexes of leaf area, root length, root area, plant height, crown growth index, tiller number, and leaf number also increased quadratically with increasing NAR similar to dry weight data. Leaf area, root length, and ...
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seed germination of southern seaoats Uniola paniculata as influenced by stratification temperature and light
Journal of environmental horticulture, 2017Co-Authors: Tyler L. Burgess, Frank A. Blazich, David L. NashAbstract:Abstract Seeds of southern seaoats (Uniola paniculata L.) were removed from storage at 4C (39F) and stratified (moist-prechilled) for 0, 15, or 30 days at 4C (39F). Following stratification, seeds were germinated at 25C (77F) or 30C (86F) or at 8/16 hr thermoperiods of 30/20C (86/68F) or 35/25C (95/77F) with daily photoperiods at each temperature of 0 (total darkness), 2, 4, 8, 12, or 24 hr. Germination was recorded every 3 days for 30 days. Light had no effect on germination. Regardless of photoperiod the influence of light was nonsignificant (P = 0.45). On the other hand, temperature and stratification were significant (P = 0.0001) and there was a significant interaction (P = 0.001) between the two parameters. Averaged across all treatments, the highest total germination was realized at 35/25C (95/77F) (60%) followed by 30/20C (86/68F) (48%), 30C (86F) (37%), and 25C (77F) (31 %). Stratification was not a requirement for germination but stratification for 15 days increased the rate of germination but no...
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influence of selected surface disinfestants fungicides and temperature on seed germination and initial growth of southern seaoats Uniola paniculata
Journal of environmental horticulture, 2017Co-Authors: Tyler L. Burgess, Frank A. Blazich, David L. Nash, Betsy RandallschadelAbstract:Abstract Seeds of southern seaoats (Uniola paniculata L.) were removed from storage at 4C (39F) and treated with the following selected surface disinfestants, fungicides, or combinations of these chemicals: nontreated (control), 1.3% sodium hypochlorite [NaOCl (chlorine bleach)], 2.6% sodium hypochlorite, RTU® (12.6% thiram + 0.34% thiabendazole), RTU®-PCNB (24% pentachloronitrobenzene), 1.3% sodium hypochlorite and RTU®, 2.6% sodium hypochlorite and RTU®, 1.3% sodium hypochlorite and RTU®-PCNB, or 2.6% sodium hypochlorite and RTU®-PCNB. Following treatment, seeds were germinated at an 8/16 hr thermoperiod of 35/20C (95/68F). The seed treatments and germination thermoperiod utilized were based on three preliminary trials that investigated the influence of selected surface disinfestants, fungicides, and temperature on seed germination of the species. Germination was recorded every 3 days for 30 days. Seed treatment was highly significant (P = 0.0001) for both total percentage germination and total percenta...
Tyler L. Burgess - One of the best experts on this subject based on the ideXlab platform.
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Influence of Selected Surface Disinfestants, Fungicides, and Temperature on Seed Germination and Initial Growth of Southern Seaoats (Uniola paniculata)
Journal of environmental horticulture, 2017Co-Authors: Tyler L. Burgess, Frank A. Blazich, David L. Nash, Betsy Randall-schadelAbstract:Abstract Seeds of southern seaoats (Uniola paniculata L.) were removed from storage at 4C (39F) and treated with the following selected surface disinfestants, fungicides, or combinations of these c...
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seed germination of southern seaoats Uniola paniculata as influenced by stratification temperature and light
Journal of environmental horticulture, 2017Co-Authors: Tyler L. Burgess, Frank A. Blazich, David L. NashAbstract:Abstract Seeds of southern seaoats (Uniola paniculata L.) were removed from storage at 4C (39F) and stratified (moist-prechilled) for 0, 15, or 30 days at 4C (39F). Following stratification, seeds were germinated at 25C (77F) or 30C (86F) or at 8/16 hr thermoperiods of 30/20C (86/68F) or 35/25C (95/77F) with daily photoperiods at each temperature of 0 (total darkness), 2, 4, 8, 12, or 24 hr. Germination was recorded every 3 days for 30 days. Light had no effect on germination. Regardless of photoperiod the influence of light was nonsignificant (P = 0.45). On the other hand, temperature and stratification were significant (P = 0.0001) and there was a significant interaction (P = 0.001) between the two parameters. Averaged across all treatments, the highest total germination was realized at 35/25C (95/77F) (60%) followed by 30/20C (86/68F) (48%), 30C (86F) (37%), and 25C (77F) (31 %). Stratification was not a requirement for germination but stratification for 15 days increased the rate of germination but no...
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influence of selected surface disinfestants fungicides and temperature on seed germination and initial growth of southern seaoats Uniola paniculata
Journal of environmental horticulture, 2017Co-Authors: Tyler L. Burgess, Frank A. Blazich, David L. Nash, Betsy RandallschadelAbstract:Abstract Seeds of southern seaoats (Uniola paniculata L.) were removed from storage at 4C (39F) and treated with the following selected surface disinfestants, fungicides, or combinations of these chemicals: nontreated (control), 1.3% sodium hypochlorite [NaOCl (chlorine bleach)], 2.6% sodium hypochlorite, RTU® (12.6% thiram + 0.34% thiabendazole), RTU®-PCNB (24% pentachloronitrobenzene), 1.3% sodium hypochlorite and RTU®, 2.6% sodium hypochlorite and RTU®, 1.3% sodium hypochlorite and RTU®-PCNB, or 2.6% sodium hypochlorite and RTU®-PCNB. Following treatment, seeds were germinated at an 8/16 hr thermoperiod of 35/20C (95/68F). The seed treatments and germination thermoperiod utilized were based on three preliminary trials that investigated the influence of selected surface disinfestants, fungicides, and temperature on seed germination of the species. Germination was recorded every 3 days for 30 days. Seed treatment was highly significant (P = 0.0001) for both total percentage germination and total percenta...
Michael E. Kane - One of the best experts on this subject based on the ideXlab platform.
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Different plant provenance same seed tolerance to abiotic stress: implications for ex situ germplasm conservation of a widely distributed coastal dune grass (Uniola paniculata L.)
Plant Growth Regulation, 2017Co-Authors: Héctor E. Pérez, Michael E. KaneAbstract:Preservation of genetic diversity within germplasm repositories represents an important tool for plant conservation. However, seeds must tolerate extreme levels of post-harvest desiccation and cold to realize benefits of ex situ storage. Factors including local climate and habitat impact expression of desiccation and freezing tolerance especially for widely distributed species. Our aim here was to understand the influence of a latitudinal gradient on seed desiccation and cryo-freezing tolerance. We sampled mature U. paniculata seeds from two geographically and genetically distinct populations then examined seed-water relations and germination following desiccation via equilibrium drying assays (0.5 to 91% RH; −797 to −12.9 MPa). Germination ability after drying and subsequent cryo-freezing treatments (−196 °C, 1 to 1440 min) was also evaluated. Seeds of both populations displayed similar reverse sigmoid moisture sorption isotherms characteristic of desiccation tolerant tissues. Furthermore, initial seed water potential (−63 and −90 MPa) was considerably lower than the lethal limit (−20 MPa) identified for desiccation sensitive tissues. Final germination (range 58–93%) and temporal patterns differed significantly between populations following desiccation and cryo-freezing stress, but these germination responses were similar to initial germination. A higher proportion of non-germinated, yet viable seeds remained for the northern compared to southern population. Location does influence germination response, but differential germination is related to seed dormancy rather than desiccation or cryo-freezing sensitivity. Ex situ conservation of U. paniculata is therefore feasible across the latitudinal gradient studied here.
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Substitution of benzyladenine with meta-topolin during shoot multiplication increases acclimatization of difficult- and easy-to-acclimatize sea oats (Uniola paniculata L.) genotypes
Plant Growth Regulation, 2009Co-Authors: Carmen Valero-aracama, Michael E. Kane, Sandra B. Wilson, Nancy L. PhilmanAbstract:Benzyladenine (BA) is the only cytokinin to effectively induce shoot multiplication in vitro between genotypes of the important dune grass species Uniola paniculata (sea oats). However, a significant genotype-specific negative carryover effect of BA on ex vitro acclimatization has been observed. In the present study, the effects of multiplication media supplemented with meta -topolin ( m T), a BA-analog, BA or no plant growth regulator, were compared on in vitro multiplication, rooting and ex vitro acclimatization using easy- and difficult-to-acclimatize sea oats genotypes. Both genotypes exhibited similar in vitro shoot dry weight, number of harvestable shoots and percent rooting when cultured under standard conditions (with 2.2 μM BA) or with an equimolar concentration of m T. In addition, both genotypes exhibited similar ex vitro leaf length and shoot production under these two culture conditions. However, ex vitro acclimatization of rooted microcuttings of the difficult-to-acclimatize genotype significantly increased when produced on shoot multiplication medium containing m T rather than BA. Meta -topolin concentrations 10 μM or greater were inhibitory to in vitro rooting and acclimatization ex vitro of both genotypes. Nevertheless, survival of the difficult-to-acclimatize genotype was significantly greater when cultured in the presence of 2.2 μM–30 μM m T, compared to 2.2 μM BA. Therefore, a potential solution to overcome the detrimental BA carryover effect on ex vitro survival in sea oats is the substitution of BA with 2.2 μM m T for Stage II shoot multiplication. Use of m T may provide an efficient method to ensure in vitro propagation of a large number of diverse sea oats genotypes for dune restoration.
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comparative growth morphology and anatomy of easy and difficult to acclimatize sea oats Uniola paniculata genotypes during in vitro culture and ex vitro acclimatization
Journal of the American Society for Horticultural Science, 2008Co-Authors: Carmen Valero Aracama, Michael E. Kane, Sandra B. Wilson, Nancy L. PhilmanAbstract:ADDITIONAL INDEX WORDS. micropropagation, native grass, transmission electron microscopy, leaf histology, dune restoration ABSTRACT. Growth and development of two sea oats (Uniola paniculata L.) genotypes with differing acclimatization capacities when transferred from in vitro to ex vitro greenhouse conditions were compared as a function of the duration of shoot multiplication and rooting stages. Anatomical and morphological development differed between genotypes in vitro. After 4, 8, and 12 weeks of Stage II culture, leaf length and shoot number were significantly greater for the easy-to-acclimatize genotype (EK 16-3) than the difficult-to-acclimatize genotype (EK 11-1).Shoot dry weights in both genotypes were greatest after 4 weeks culture. Browning and dying of tissue steadily increased with time. Shoot number per plantlet increased from Week 4 to 8 in both genotypes but decreased after 12 weeks. Once transferredtoStageIIIculturefor6weeks,significantdifferencesinrootarchitecture andmorphologywereobserved between the two genotypes. EK 16-3 plantlets developed short but numerous roots and ''grass-like'' leaves with fully expanded blades. Conversely, EK 11-1 plantlets developed few long roots and ''lance-like'' leaves, which were short, thick, and without expanded blades. Anatomical and morphological development during Stage III differed between culture duration and genotypes. Shoot and root dry weights of both genotypes increased during 3, 6, and 9 weeks of culture. Shoot dry weights of EK 16-3 plantlets were lower at 3 weeks but higher at 9 weeks than EK 11-1. Conversely, root dry weights were higher for EK 11-1 than for EK 16-3 plantlets throughout Stage III culture. Anatomical observations of EK 11-1 plantlets using light and electron microscopy correlated poor ex vitro acclimatization and poor survival with abnormal tissue organization, stomatal aperture blockage, and thylakoid membrane disruption.
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Influence of in vitro growth conditions on in vitro and ex vitro photosynthetic rates of easy- and difficult-to-acclimatize sea oats (Uniola paniculata L.) genotypes
In Vitro Cellular & Developmental Biology - Plant, 2007Co-Authors: Carmen Valero-aracama, Michael E. Kane, Sandra B. Wilson, Nancy L. PhilmanAbstract:Net photosynthetic rates ( P _n) of easy (EK 16-3) and difficult-to-acclimatize (EK 11-1) sea oats genotypes were examined under the following culture conditions: (1) photoautotrophic [sugar-free medium, high photosynthetic photon flux (PPF), high vessel ventilation rates and CO_2 enrichment, (PA)]; (2) modified photomixotrophic [sugar-containing medium diluted with sugar-free medium over time, high PPF, and high vessel ventilation rates (PM)]; (3) modified photomixotrophic enriched [same as PM with CO_2 enrichment, (PME)]; or (4) conventional photomixotrophic [sugar-containing medium, low PPF, and low vessel ventilation rates (control)]. Regardless of genotype, plantlets cultured under PA conditions died within 2 wk, whereas under PM and PME conditions, plantlets increased their P _n. After 6 wk, P _n per gram dry weight was 1.7 times greater in EK 16-3 than EK 11-1 plantlets cultured under PME conditions. In vitro -produced leaves of EK 16-3 plantlets were elongated with expanded blades, whereas EK 11-1 produced short leaves without expanded blades, especially under control conditions. After in vitro culture, EK 16-3 PME plantlets exhibited the highest dry weights among treatments. EK 16-3 PME and EK 16-3 PM had similarly high survivability, shoot and root dry weights and leaf lengths ex vitro compared to EK 16-3 control and EK 11-1 PM and PME plantlets. Ex vitro growth, survivability and P _n per leaf area of either genotype were not affected by CO_2 enrichment under modified photomixotrophic conditions. These results suggest that growth and survivability of sea oats genotypes with different acclimatization capacities can be enhanced by optimizing culture conditions.
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Photosynthetic and carbohydrate status of easy-and difficult-to-acclimatize sea oats (Uniola paniculata L.) genotypes during In vitro culture and Ex vitro acclimatization
In Vitro Cellular & Developmental Biology - Plant, 2006Co-Authors: Carmen Valero-aracama, Michael E. Kane, Sandra B. Wilson, Joseph C. Vu, Joan Anderson, Nancy L. PhilmanAbstract:The photosynthetic and carbohydrate status of an easy-to-acclimatize (EK 16-3) and a difficult-to-acclimatize (EK 11-1) genotype of Uniola paniculata L. (sea oats), a native dune species of the southeastern US, were evaluated during in vitro culture and ex vitro acclimatization. Net photosynthetic rate was eight times greater for EK 16-3 than EK 11-1 plantlets after ex vitro transfer. In vitro -produced leaves were morphologically similar to ex vitro -produced leaves and exhibited similar photosynthetic competence. EK 11-1 plantlets exhibited greater transpiration rates at the time of ex vitro transfer than EK 16-3 plantlets. However, the small magnitude of this difference, although significant, indicated that control of water loss was probably not the main cause for poor acclimatization of EK 11-1 plantlets. Carbohydrate analysis in vitro revealed that EK 16-3 plantlets utilized leaf starch reserves more rapidly than EK 11-1 plantlets. Starch utilization correlated with the development of leaves with expanded leaf blades during in vitro rooting in EK 16-3 plantlets. After ex vitro transfer, both genotypes exhibited significant decreases of starch and soluble sugar content in shoots and roots. However, the higher photosynthetic ability of shoots in EK 16-3 resulted in greater accumulation of shoot soluble sugars than EK 11-1 after 2-wk ex vitro culture. After 6-wk in vitro rooting, there were significantly higher chlorophyll and soluble protein contents, ribulose 1,5-bisphosphate carboxylase (rubisco) and phosphoenolpyruvate carboxylase activities in EK 16-3 than EK 11-1 shoots. These differences also correlated with the development of anatomical and morphological leaf features in EK 16-3 similar to those of greenhouse-produced leaves.
