The Experts below are selected from a list of 273 Experts worldwide ranked by ideXlab platform
John R. Asplin - One of the best experts on this subject based on the ideXlab platform.
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Fecal transplant modifies Urine Chemistry risk factors for urinary stone disease
Physiological reports, 2019Co-Authors: Joshua M. Stern, John R. Asplin, Ignacio Granja, Marcia Urban-maldonado, Mykhaylo Usyk, Daniel Schoenfeld, Kelvin P. Davies, Ilir Agalliu, Robert D. Burk, Sylvia O. SuadicaniAbstract:Urinary stone disease (USD) is a major health concern. There is a need for new treatment modalities. Recently, our group provided evidence for an association between the GMB composition and USD. The accessibility of the Gut Microbiome (GMB) makes it an attractive target for investigation and therefore, in these studies we have evaluated the extent to which the whole gut microbial community in fecal transplants can affect urinary stone risk parameters in an animal model. Fresh fecal pellets were collected from Zucker lean rats, homogenized in PBS (100 mg/mL), filtered through a 70 μm strainer and then orally gavaged into C57BL/6NTac germ-free mice. Twenty-four hours Urine collections and GMB analysis were performed over time for 1 month. Kidney and gut tissue were harvested from transplanted mice for western blot analysis of expression levels of the Slc26a6 transporter involved in oxalate balance. Urinary calcium decreased after fecal transplant by 55% (P < 0.001). Urinary oxalate levels were on average 24% lower than baseline levels (P < 0.001). Clostridiaceae family was negatively correlated with urinary oxalate at 4 weeks after transplant (r = -0.83, P < 0.01). There was a 0.6 unit average increase in urinary pH from a baseline of 5.85 (SE ± 0.028) to 6.49 (SE ± 0.04) (P < 0.001) after transplant. There was a concomitant 29% increase in gastrointestinal alkali absorption (P < 0.001) 4-weeks after fecal transplant. Slc26a6 expression increased by 90% in the cecum after transplant. Our results suggest that the gut microbiome may impact metabolism, alters urinary Chemistry, and thereby may influence USD; the accessibility of the GMB can potentially be leveraged for therapeutic interventions.
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A Pilot Study of the Effect of Sodium Thiosulfate on Urinary Lithogenicity and Associated Metabolic Acid Load in Non-Stone
2016Co-Authors: Stone Formers, John R. Asplin, Onyeka W Okonkwo, Ruchika Batwara, Ignacio Granja, David S. GoldfarbAbstract:Background and Objectives: Sodium thiosulfate (STS) reduced calcium stone formation in both humans and genetic hypercalciuric stone forming (GHS) rats. We sought to measure Urine Chemistry changes resulting from STS administration in people. Design, Setting, Participants & Measurements: STS was given to healthy and hypercalciuric stone forming adults. Five normal non-stone forming adults (mean age 33 years), and 5 people with idiopathic hypercalciuria and calcium kidney stones (mean age 66 years) participated. Two baseline 24-hour Urine collections were performed on days 2 and 3 of 3 days of self-selected diets. Subjects then drank STS 10 mmol twice a day for 7 days and did Urine collections while repeating the self-selected diet. Results were compared by non-parametric Wilcoxon signed rank test. The primary outcome was the resulting change in Urine Chemistry. Results: STS administration did not cause a significant change in urinary calcium excretion in either group. In both groups, 24 hour urinary ammonium (P = 0.005) and sulfate excretion (P = 0.007) increased, and urinary pH fell (P = 0.005); citrate excretion fell (P,0.05) in hypercalciuric participants but not in non-stone formers. Among stone formers with hypercalciuria, 3 of 5 patients had measurement of serum HCO3 concentration after the STS period: it did not change. The net effect was an increase in supersaturation of uric acid, and no change in supersaturation of calcium oxalate or calcium phosphate
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How Much Information is Lost When You Only Collect One 24-Hour Urine Sample during the Initial Metabolic Evaluation?
The Journal of urology, 2016Co-Authors: Abdulrahman F. Alruwaily, John R. Asplin, Casey A. Dauw, Maggie Bierlein, Phyllis Yan, Khurshid R. Ghani, J. Stuart Wolf, John M. HollingsworthAbstract:Purpose: During the initial metabolic evaluation the need for 1 vs 2, 24-hour Urine collections is debated. While data suggest that mean Urine Chemistry measures are similar on consecutive samples, it remains unclear how much, if any, information is lost when only 1 sample is collected.Materials and Methods: Using analytical files from Litholink Corporation® (1995 to 2013) we identified adults with kidney stones who underwent initial metabolic testing. Next we determined the subset of patients who collected 2, 24-hour Urine samples with Urine creatinine varying by 10% or less during a 7-day time window. We then examined the degree of variability in Urine Chemistry profiles. Specifically we calculated the mean absolute value of the difference between samples as well as the percent difference for individual Urine parameters.Results: We identified 70,192 patients meeting our eligibility criteria. While the overall means for individual Urine parameters did not vary between samples, the percent difference betw...
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Effects of Sex on Intra-Individual Variance in Urinary Solutes in Stone-Formers Collected from a Single Clinical Laboratory.
PloS one, 2013Co-Authors: Guy M. L. Perry, Steven J. Scheinman, John R. AsplinAbstract:Background/Aims Our work in a rodent model of urinary calcium suggests genetic and gender effects on increased residual variability in Urine chemistries. Based on these findings, we hypothesized that sex would similarly be associated with residual variation in human Urine solutes. Sex-related effects on residuals might affect the establishment of physiological baselines and error in medical assays. Methods We tested the effects of sex on residual variation in Urine Chemistry by estimating coefficients of variation (CV) for urinary solutes in paired sequential 24-h Urines (≤72 hour interval) in 6,758 females and 9,024 males aged 16–80 submitted to a clinical laboratory. Results Females had higher CVs than males for urinary phosphorus overall at the False Discovery Rate (P 0.3). Males had higher CVs for citrate (P
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a pilot study of the effect of sodium thiosulfate on urinary lithogenicity and associated metabolic acid load in non stone formers and stone formers with hypercalciuria
PLOS ONE, 2013Co-Authors: Onyeka W Okonkwo, John R. Asplin, Ruchika Batwara, Ignacio Granja, David S. GoldfarbAbstract:Background and Objectives Sodium thiosulfate (STS) reduced calcium stone formation in both humans and genetic hypercalciuric stone forming (GHS) rats. We sought to measure Urine Chemistry changes resulting from STS administration in people. Design, Setting, Participants & Measurements STS was given to healthy and hypercalciuric stone forming adults. Five normal non-stone forming adults (mean age 33 years), and 5 people with idiopathic hypercalciuria and calcium kidney stones (mean age 66 years) participated. Two baseline 24-hour Urine collections were performed on days 2 and 3 of 3 days of self-selected diets. Subjects then drank STS 10 mmol twice a day for 7 days and did Urine collections while repeating the self-selected diet. Results were compared by non-parametric Wilcoxon signed rank test. The primary outcome was the resulting change in Urine Chemistry. Results STS administration did not cause a significant change in urinary calcium excretion in either group. In both groups, 24 hour urinary ammonium (P = 0.005) and sulfate excretion (P = 0.007) increased, and urinary pH fell (P = 0.005); citrate excretion fell (P<0.05) in hypercalciuric participants but not in non-stone formers. Among stone formers with hypercalciuria, 3 of 5 patients had measurement of serum HCO3 concentration after the STS period: it did not change. The net effect was an increase in supersaturation of uric acid, and no change in supersaturation of calcium oxalate or calcium phosphate. Conclusions The basis for studies demonstrating that STS prevented stones in rats and people was not reflected by the changes in Urine Chemistry reported here. Although serum HCO3 did not change, Urine tests suggested an acid load in both non-stone forming and hypercalciuric stone-forming participants. The long term safety of STS needs to be determined before the drug can be tested in humans for long-term prevention of stone recurrence.
Jui-yi Yang - One of the best experts on this subject based on the ideXlab platform.
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comparison of three automated urinalysis systems bayer clinitek atlas roche urisys 2400 and arkray aution max for testing Urine Chemistry and detection of bacteriuria
Clinica Chimica Acta, 2007Co-Authors: Tzu-i Chien, Jau-tsuen Kao, Tai-fen Lee, Chia-yu Chang, Chao-wei Liu, Chun-hsien Lee, Shiao-ni Yan, Jui-yi Yang, Pochuang Lin, Meiju WangAbstract:Abstract Background Our study is aimed to determine the performance of 3 automated urinalysis systems—Clinitek Atlas, Urisys 2400 and Aution Max. Methods One thousand Urine specimens were analyzed with the 3 automated systems. The results of the 3 assays were compared for testing Urine Chemistry and evaluating the capacity of leukocyte esterase and nitrite to detect bacteriuria. Results The correlation between the 3 instruments represented as within 1 grading difference was better between the Atlas and Aution Max systems for pH, blood, glucose, urobilinogen, ketone and specific gravity. For protein and nitrite, better correlation was observed between the Atlas and Urisys 2400, while the Aution Max and Urisys 2400 conveyed better correlation for bilirubin and white blood cells. The sensitivity and specificity of both the leukocyte esterase and nitrite in screening for significant bacteriuria were 71.7, 58.9, 70.8% and 99.1, 99.1 and 97.2%, for the Clinitek Atlas, Aution Max and Urisys 2400, respectively. Conclusions The automated urinalysis systems demonstrate acceptable correlations with each other in Urine chemistries, especially between the Clinitek Atlas and Aution Max systems on the majority of items. The specificity and negative predictive value of leukocyte esterase and nitrite of the 3 instruments for screening of significant bacteriuria were sufficient to avoid unnecessary Urine culture.
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Comparison of three automated urinalysis systems--Bayer Clinitek Atlas, Roche Urisys 2400 and Arkray Aution Max for testing Urine Chemistry and detection of bacteriuria.
Clinica chimica acta; international journal of clinical chemistry, 2006Co-Authors: Tzu-i Chien, Jau-tsuen Kao, Tai-fen Lee, Chia-yu Chang, Chao-wei Liu, Chun-hsien Lee, Shiao-ni Yan, Jui-yi YangAbstract:Our study is aimed to determine the performance of 3 automated urinalysis systems-Clinitek Atlas, Urisys 2400 and Aution Max. One thousand Urine specimens were analyzed with the 3 automated systems. The results of the 3 assays were compared for testing Urine Chemistry and evaluating the capacity of leukocyte esterase and nitrite to detect bacteriuria. The correlation between the 3 instruments represented as within 1 grading difference was better between the Atlas and Aution Max systems for pH, blood, glucose, urobilinogen, ketone and specific gravity. For protein and nitrite, better correlation was observed between the Atlas and Urisys 2400, while the Aution Max and Urisys 2400 conveyed better correlation for bilirubin and white blood cells. The sensitivity and specificity of both the leukocyte esterase and nitrite in screening for significant bacteriuria were 71.7, 58.9, 70.8% and 99.1, 99.1 and 97.2%, for the Clinitek Atlas, Aution Max and Urisys 2400, respectively. The automated urinalysis systems demonstrate acceptable correlations with each other in Urine chemistries, especially between the Clinitek Atlas and Aution Max systems on the majority of items. The specificity and negative predictive value of leukocyte esterase and nitrite of the 3 instruments for screening of significant bacteriuria were sufficient to avoid unnecessary Urine culture.
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Comparison of three automated urinalysis systems—Bayer Clinitek Atlas, Roche Urisys 2400 and Arkray Aution Max for testing Urine Chemistry and detection of bacteriuria
Clinica Chimica Acta, 2006Co-Authors: Tzu-i Chien, Jau-tsuen Kao, Tai-fen Lee, Chia-yu Chang, Chao-wei Liu, Chun-hsien Lee, Shiao-ni Yan, Jui-yi YangAbstract:Abstract Background Our study is aimed to determine the performance of 3 automated urinalysis systems—Clinitek Atlas, Urisys 2400 and Aution Max. Methods One thousand Urine specimens were analyzed with the 3 automated systems. The results of the 3 assays were compared for testing Urine Chemistry and evaluating the capacity of leukocyte esterase and nitrite to detect bacteriuria. Results The correlation between the 3 instruments represented as within 1 grading difference was better between the Atlas and Aution Max systems for pH, blood, glucose, urobilinogen, ketone and specific gravity. For protein and nitrite, better correlation was observed between the Atlas and Urisys 2400, while the Aution Max and Urisys 2400 conveyed better correlation for bilirubin and white blood cells. The sensitivity and specificity of both the leukocyte esterase and nitrite in screening for significant bacteriuria were 71.7, 58.9, 70.8% and 99.1, 99.1 and 97.2%, for the Clinitek Atlas, Aution Max and Urisys 2400, respectively. Conclusions The automated urinalysis systems demonstrate acceptable correlations with each other in Urine chemistries, especially between the Clinitek Atlas and Aution Max systems on the majority of items. The specificity and negative predictive value of leukocyte esterase and nitrite of the 3 instruments for screening of significant bacteriuria were sufficient to avoid unnecessary Urine culture.
David S. Goldfarb - One of the best experts on this subject based on the ideXlab platform.
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The use of antibiotics and risk of kidney stones.
Current opinion in nephrology and hypertension, 2019Co-Authors: Shivam Joshi, David S. GoldfarbAbstract:Purpose of reviewThe effect of the intestinal microbiome on Urine Chemistry and lithogenicity has been a popular topic. Here we review the evidence for exposure to antibiotics increasing the risk of nephrolithiasis.Recent findingsStudies of the intestinal microbiome have focused on Oxalobacter formi
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Empiric therapy for kidney stones.
Urolithiasis, 2018Co-Authors: David S. GoldfarbAbstract:Careful phenotyping of patients to classify those with kidney stones has a long and important history in revealing the chemical basis for stone formation. Advances in our genetic understanding of kidney stones will lead to incredible insights regarding the pathophysiology of this common disorder. At this time, both evaluation of Urine Chemistry and genotyping of patients are extremely useful in the setting of a university and research-based kidney stone clinic. For much of the world, in a more clinically focused setting, these techniques are neither available nor absolutely necessary. Careful implementation of an empiric prescription based on stone composition would have an important effect to reduce stone recurrence in the world's many stone formers. Increased fluid intake, generic dietary manipulations, and prescription of potassium citrate and thiazides are all appropriate empiric therapies for people with calcium and uric acid kidney stones.
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A Pilot Study of the Effect of Sodium Thiosulfate on Urinary Lithogenicity and Associated Metabolic Acid Load in Non-Stone
2016Co-Authors: Stone Formers, John R. Asplin, Onyeka W Okonkwo, Ruchika Batwara, Ignacio Granja, David S. GoldfarbAbstract:Background and Objectives: Sodium thiosulfate (STS) reduced calcium stone formation in both humans and genetic hypercalciuric stone forming (GHS) rats. We sought to measure Urine Chemistry changes resulting from STS administration in people. Design, Setting, Participants & Measurements: STS was given to healthy and hypercalciuric stone forming adults. Five normal non-stone forming adults (mean age 33 years), and 5 people with idiopathic hypercalciuria and calcium kidney stones (mean age 66 years) participated. Two baseline 24-hour Urine collections were performed on days 2 and 3 of 3 days of self-selected diets. Subjects then drank STS 10 mmol twice a day for 7 days and did Urine collections while repeating the self-selected diet. Results were compared by non-parametric Wilcoxon signed rank test. The primary outcome was the resulting change in Urine Chemistry. Results: STS administration did not cause a significant change in urinary calcium excretion in either group. In both groups, 24 hour urinary ammonium (P = 0.005) and sulfate excretion (P = 0.007) increased, and urinary pH fell (P = 0.005); citrate excretion fell (P,0.05) in hypercalciuric participants but not in non-stone formers. Among stone formers with hypercalciuria, 3 of 5 patients had measurement of serum HCO3 concentration after the STS period: it did not change. The net effect was an increase in supersaturation of uric acid, and no change in supersaturation of calcium oxalate or calcium phosphate
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a pilot study of the effect of sodium thiosulfate on urinary lithogenicity and associated metabolic acid load in non stone formers and stone formers with hypercalciuria
PLOS ONE, 2013Co-Authors: Onyeka W Okonkwo, John R. Asplin, Ruchika Batwara, Ignacio Granja, David S. GoldfarbAbstract:Background and Objectives Sodium thiosulfate (STS) reduced calcium stone formation in both humans and genetic hypercalciuric stone forming (GHS) rats. We sought to measure Urine Chemistry changes resulting from STS administration in people. Design, Setting, Participants & Measurements STS was given to healthy and hypercalciuric stone forming adults. Five normal non-stone forming adults (mean age 33 years), and 5 people with idiopathic hypercalciuria and calcium kidney stones (mean age 66 years) participated. Two baseline 24-hour Urine collections were performed on days 2 and 3 of 3 days of self-selected diets. Subjects then drank STS 10 mmol twice a day for 7 days and did Urine collections while repeating the self-selected diet. Results were compared by non-parametric Wilcoxon signed rank test. The primary outcome was the resulting change in Urine Chemistry. Results STS administration did not cause a significant change in urinary calcium excretion in either group. In both groups, 24 hour urinary ammonium (P = 0.005) and sulfate excretion (P = 0.007) increased, and urinary pH fell (P = 0.005); citrate excretion fell (P<0.05) in hypercalciuric participants but not in non-stone formers. Among stone formers with hypercalciuria, 3 of 5 patients had measurement of serum HCO3 concentration after the STS period: it did not change. The net effect was an increase in supersaturation of uric acid, and no change in supersaturation of calcium oxalate or calcium phosphate. Conclusions The basis for studies demonstrating that STS prevented stones in rats and people was not reflected by the changes in Urine Chemistry reported here. Although serum HCO3 did not change, Urine tests suggested an acid load in both non-stone forming and hypercalciuric stone-forming participants. The long term safety of STS needs to be determined before the drug can be tested in humans for long-term prevention of stone recurrence.
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A pilot study of the effect of sodium thiosulfate on urinary lithogenicity and associated metabolic acid load in non-stone formers and stone formers with hypercalciuria.
PloS one, 2013Co-Authors: Onyeka W Okonkwo, John R. Asplin, Ruchika Batwara, Ignacio Granja, David S. GoldfarbAbstract:Background and Objectives Sodium thiosulfate (STS) reduced calcium stone formation in both humans and genetic hypercalciuric stone forming (GHS) rats. We sought to measure Urine Chemistry changes resulting from STS administration in people. Design, Setting, Participants & Measurements STS was given to healthy and hypercalciuric stone forming adults. Five normal non-stone forming adults (mean age 33 years), and 5 people with idiopathic hypercalciuria and calcium kidney stones (mean age 66 years) participated. Two baseline 24-hour Urine collections were performed on days 2 and 3 of 3 days of self-selected diets. Subjects then drank STS 10 mmol twice a day for 7 days and did Urine collections while repeating the self-selected diet. Results were compared by non-parametric Wilcoxon signed rank test. The primary outcome was the resulting change in Urine Chemistry. Results STS administration did not cause a significant change in urinary calcium excretion in either group. In both groups, 24 hour urinary ammonium (P = 0.005) and sulfate excretion (P = 0.007) increased, and urinary pH fell (P = 0.005); citrate excretion fell (P
Tzu-i Chien - One of the best experts on this subject based on the ideXlab platform.
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comparison of three automated urinalysis systems bayer clinitek atlas roche urisys 2400 and arkray aution max for testing Urine Chemistry and detection of bacteriuria
Clinica Chimica Acta, 2007Co-Authors: Tzu-i Chien, Jau-tsuen Kao, Tai-fen Lee, Chia-yu Chang, Chao-wei Liu, Chun-hsien Lee, Shiao-ni Yan, Jui-yi Yang, Pochuang Lin, Meiju WangAbstract:Abstract Background Our study is aimed to determine the performance of 3 automated urinalysis systems—Clinitek Atlas, Urisys 2400 and Aution Max. Methods One thousand Urine specimens were analyzed with the 3 automated systems. The results of the 3 assays were compared for testing Urine Chemistry and evaluating the capacity of leukocyte esterase and nitrite to detect bacteriuria. Results The correlation between the 3 instruments represented as within 1 grading difference was better between the Atlas and Aution Max systems for pH, blood, glucose, urobilinogen, ketone and specific gravity. For protein and nitrite, better correlation was observed between the Atlas and Urisys 2400, while the Aution Max and Urisys 2400 conveyed better correlation for bilirubin and white blood cells. The sensitivity and specificity of both the leukocyte esterase and nitrite in screening for significant bacteriuria were 71.7, 58.9, 70.8% and 99.1, 99.1 and 97.2%, for the Clinitek Atlas, Aution Max and Urisys 2400, respectively. Conclusions The automated urinalysis systems demonstrate acceptable correlations with each other in Urine chemistries, especially between the Clinitek Atlas and Aution Max systems on the majority of items. The specificity and negative predictive value of leukocyte esterase and nitrite of the 3 instruments for screening of significant bacteriuria were sufficient to avoid unnecessary Urine culture.
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Comparison of three automated urinalysis systems--Bayer Clinitek Atlas, Roche Urisys 2400 and Arkray Aution Max for testing Urine Chemistry and detection of bacteriuria.
Clinica chimica acta; international journal of clinical chemistry, 2006Co-Authors: Tzu-i Chien, Jau-tsuen Kao, Tai-fen Lee, Chia-yu Chang, Chao-wei Liu, Chun-hsien Lee, Shiao-ni Yan, Jui-yi YangAbstract:Our study is aimed to determine the performance of 3 automated urinalysis systems-Clinitek Atlas, Urisys 2400 and Aution Max. One thousand Urine specimens were analyzed with the 3 automated systems. The results of the 3 assays were compared for testing Urine Chemistry and evaluating the capacity of leukocyte esterase and nitrite to detect bacteriuria. The correlation between the 3 instruments represented as within 1 grading difference was better between the Atlas and Aution Max systems for pH, blood, glucose, urobilinogen, ketone and specific gravity. For protein and nitrite, better correlation was observed between the Atlas and Urisys 2400, while the Aution Max and Urisys 2400 conveyed better correlation for bilirubin and white blood cells. The sensitivity and specificity of both the leukocyte esterase and nitrite in screening for significant bacteriuria were 71.7, 58.9, 70.8% and 99.1, 99.1 and 97.2%, for the Clinitek Atlas, Aution Max and Urisys 2400, respectively. The automated urinalysis systems demonstrate acceptable correlations with each other in Urine chemistries, especially between the Clinitek Atlas and Aution Max systems on the majority of items. The specificity and negative predictive value of leukocyte esterase and nitrite of the 3 instruments for screening of significant bacteriuria were sufficient to avoid unnecessary Urine culture.
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Comparison of three automated urinalysis systems—Bayer Clinitek Atlas, Roche Urisys 2400 and Arkray Aution Max for testing Urine Chemistry and detection of bacteriuria
Clinica Chimica Acta, 2006Co-Authors: Tzu-i Chien, Jau-tsuen Kao, Tai-fen Lee, Chia-yu Chang, Chao-wei Liu, Chun-hsien Lee, Shiao-ni Yan, Jui-yi YangAbstract:Abstract Background Our study is aimed to determine the performance of 3 automated urinalysis systems—Clinitek Atlas, Urisys 2400 and Aution Max. Methods One thousand Urine specimens were analyzed with the 3 automated systems. The results of the 3 assays were compared for testing Urine Chemistry and evaluating the capacity of leukocyte esterase and nitrite to detect bacteriuria. Results The correlation between the 3 instruments represented as within 1 grading difference was better between the Atlas and Aution Max systems for pH, blood, glucose, urobilinogen, ketone and specific gravity. For protein and nitrite, better correlation was observed between the Atlas and Urisys 2400, while the Aution Max and Urisys 2400 conveyed better correlation for bilirubin and white blood cells. The sensitivity and specificity of both the leukocyte esterase and nitrite in screening for significant bacteriuria were 71.7, 58.9, 70.8% and 99.1, 99.1 and 97.2%, for the Clinitek Atlas, Aution Max and Urisys 2400, respectively. Conclusions The automated urinalysis systems demonstrate acceptable correlations with each other in Urine chemistries, especially between the Clinitek Atlas and Aution Max systems on the majority of items. The specificity and negative predictive value of leukocyte esterase and nitrite of the 3 instruments for screening of significant bacteriuria were sufficient to avoid unnecessary Urine culture.
Jau-tsuen Kao - One of the best experts on this subject based on the ideXlab platform.
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comparison of three automated urinalysis systems bayer clinitek atlas roche urisys 2400 and arkray aution max for testing Urine Chemistry and detection of bacteriuria
Clinica Chimica Acta, 2007Co-Authors: Tzu-i Chien, Jau-tsuen Kao, Tai-fen Lee, Chia-yu Chang, Chao-wei Liu, Chun-hsien Lee, Shiao-ni Yan, Jui-yi Yang, Pochuang Lin, Meiju WangAbstract:Abstract Background Our study is aimed to determine the performance of 3 automated urinalysis systems—Clinitek Atlas, Urisys 2400 and Aution Max. Methods One thousand Urine specimens were analyzed with the 3 automated systems. The results of the 3 assays were compared for testing Urine Chemistry and evaluating the capacity of leukocyte esterase and nitrite to detect bacteriuria. Results The correlation between the 3 instruments represented as within 1 grading difference was better between the Atlas and Aution Max systems for pH, blood, glucose, urobilinogen, ketone and specific gravity. For protein and nitrite, better correlation was observed between the Atlas and Urisys 2400, while the Aution Max and Urisys 2400 conveyed better correlation for bilirubin and white blood cells. The sensitivity and specificity of both the leukocyte esterase and nitrite in screening for significant bacteriuria were 71.7, 58.9, 70.8% and 99.1, 99.1 and 97.2%, for the Clinitek Atlas, Aution Max and Urisys 2400, respectively. Conclusions The automated urinalysis systems demonstrate acceptable correlations with each other in Urine chemistries, especially between the Clinitek Atlas and Aution Max systems on the majority of items. The specificity and negative predictive value of leukocyte esterase and nitrite of the 3 instruments for screening of significant bacteriuria were sufficient to avoid unnecessary Urine culture.
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Comparison of three automated urinalysis systems--Bayer Clinitek Atlas, Roche Urisys 2400 and Arkray Aution Max for testing Urine Chemistry and detection of bacteriuria.
Clinica chimica acta; international journal of clinical chemistry, 2006Co-Authors: Tzu-i Chien, Jau-tsuen Kao, Tai-fen Lee, Chia-yu Chang, Chao-wei Liu, Chun-hsien Lee, Shiao-ni Yan, Jui-yi YangAbstract:Our study is aimed to determine the performance of 3 automated urinalysis systems-Clinitek Atlas, Urisys 2400 and Aution Max. One thousand Urine specimens were analyzed with the 3 automated systems. The results of the 3 assays were compared for testing Urine Chemistry and evaluating the capacity of leukocyte esterase and nitrite to detect bacteriuria. The correlation between the 3 instruments represented as within 1 grading difference was better between the Atlas and Aution Max systems for pH, blood, glucose, urobilinogen, ketone and specific gravity. For protein and nitrite, better correlation was observed between the Atlas and Urisys 2400, while the Aution Max and Urisys 2400 conveyed better correlation for bilirubin and white blood cells. The sensitivity and specificity of both the leukocyte esterase and nitrite in screening for significant bacteriuria were 71.7, 58.9, 70.8% and 99.1, 99.1 and 97.2%, for the Clinitek Atlas, Aution Max and Urisys 2400, respectively. The automated urinalysis systems demonstrate acceptable correlations with each other in Urine chemistries, especially between the Clinitek Atlas and Aution Max systems on the majority of items. The specificity and negative predictive value of leukocyte esterase and nitrite of the 3 instruments for screening of significant bacteriuria were sufficient to avoid unnecessary Urine culture.
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Comparison of three automated urinalysis systems—Bayer Clinitek Atlas, Roche Urisys 2400 and Arkray Aution Max for testing Urine Chemistry and detection of bacteriuria
Clinica Chimica Acta, 2006Co-Authors: Tzu-i Chien, Jau-tsuen Kao, Tai-fen Lee, Chia-yu Chang, Chao-wei Liu, Chun-hsien Lee, Shiao-ni Yan, Jui-yi YangAbstract:Abstract Background Our study is aimed to determine the performance of 3 automated urinalysis systems—Clinitek Atlas, Urisys 2400 and Aution Max. Methods One thousand Urine specimens were analyzed with the 3 automated systems. The results of the 3 assays were compared for testing Urine Chemistry and evaluating the capacity of leukocyte esterase and nitrite to detect bacteriuria. Results The correlation between the 3 instruments represented as within 1 grading difference was better between the Atlas and Aution Max systems for pH, blood, glucose, urobilinogen, ketone and specific gravity. For protein and nitrite, better correlation was observed between the Atlas and Urisys 2400, while the Aution Max and Urisys 2400 conveyed better correlation for bilirubin and white blood cells. The sensitivity and specificity of both the leukocyte esterase and nitrite in screening for significant bacteriuria were 71.7, 58.9, 70.8% and 99.1, 99.1 and 97.2%, for the Clinitek Atlas, Aution Max and Urisys 2400, respectively. Conclusions The automated urinalysis systems demonstrate acceptable correlations with each other in Urine chemistries, especially between the Clinitek Atlas and Aution Max systems on the majority of items. The specificity and negative predictive value of leukocyte esterase and nitrite of the 3 instruments for screening of significant bacteriuria were sufficient to avoid unnecessary Urine culture.
