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Marc R. Knight - One of the best experts on this subject based on the ideXlab platform.
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A C-repeat binding factor transcriptional activator (CBF/DREB1) from European bilberry (Vaccinium myrtillus) induces freezing tolerance when expressed in Arabidopsis thaliana.
PloS one, 2013Co-Authors: Rachael J. Oakenfull, Robert Baxter, Marc R. KnightAbstract:Freezing stress affects all plants from temperate zones to the poles. Global climate change means such freezing events are becoming less predictable. This in turn reduces the ability of plants to predict the approaching low temperatures and cold acclimate. This has consequences for crop yields and distribution of wild plant species. C-repeat binding factors (CBFs) are transcription factors previously shown to play a vital role in the acclimation process of Arabidopsis thaliana, controlling the expression of hundreds of genes whose products are necessary for freezing tolerance. Work in other plant species cements CBFs as key determinants in the trait of freezing tolerance in higher plants. To test the function of CBFs from highly freezing tolerant plants species we cloned and sequenced CBF transcription factors from three Vaccinium species (Vaccinium myrtillus, Vaccinium uliginosum and Vaccinium vitis-idaea) which we collected in the Arctic. We tested the activity of CBF transcription factors from the three Vaccinium species by producing transgenic Arabidopsis lines overexpressing them. Only the Vaccinium myrtillus CBF was able to substantially activate COR (CBF-target) gene expression in the absence of cold. Correspondingly, only the lines expressing the Vaccinium myrtillus CBF were constitutively freezing tolerant. The basis for the differences in potency of the three Vaccinium CBFs was tested by observing cellular localisation and protein levels. All three CBFs were correctly targeted to the nucleus, but Vaccinium uliginosum CBF appeared to be relatively unstable. The reasons for lack of potency for Vaccinium vitis-idaea CBF were not due to stability or targeting, and we speculate that this was due to altered transcription factor function.
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a c repeat binding factor transcriptional activator cbf dreb1 from european bilberry Vaccinium myrtillus induces freezing tolerance when expressed in arabidopsis thaliana
PLOS ONE, 2013Co-Authors: Rachael J. Oakenfull, Robert Baxter, Marc R. KnightAbstract:Freezing stress affects all plants from temperate zones to the poles. Global climate change means such freezing events are becoming less predictable. This in turn reduces the ability of plants to predict the approaching low temperatures and cold acclimate. This has consequences for crop yields and distribution of wild plant species. C-repeat binding factors (CBFs) are transcription factors previously shown to play a vital role in the acclimation process of Arabidopsis thaliana, controlling the expression of hundreds of genes whose products are necessary for freezing tolerance. Work in other plant species cements CBFs as key determinants in the trait of freezing tolerance in higher plants. To test the function of CBFs from highly freezing tolerant plants species we cloned and sequenced CBF transcription factors from three Vaccinium species (Vaccinium myrtillus, Vaccinium uliginosum and Vaccinium vitis-idaea) which we collected in the Arctic. We tested the activity of CBF transcription factors from the three Vaccinium species by producing transgenic Arabidopsis lines overexpressing them. Only the Vaccinium myrtillus CBF was able to substantially activate COR (CBF-target) gene expression in the absence of cold. Correspondingly, only the lines expressing the Vaccinium myrtillus CBF were constitutively freezing tolerant. The basis for the differences in potency of the three Vaccinium CBFs was tested by observing cellular localisation and protein levels. All three CBFs were correctly targeted to the nucleus, but Vaccinium uliginosum CBF appeared to be relatively unstable. The reasons for lack of potency for Vaccinium vitis-idaea CBF were not due to stability or targeting, and we speculate that this was due to altered transcription factor function.
Rachael J. Oakenfull - One of the best experts on this subject based on the ideXlab platform.
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A C-repeat binding factor transcriptional activator (CBF/DREB1) from European bilberry (Vaccinium myrtillus) induces freezing tolerance when expressed in Arabidopsis thaliana.
PloS one, 2013Co-Authors: Rachael J. Oakenfull, Robert Baxter, Marc R. KnightAbstract:Freezing stress affects all plants from temperate zones to the poles. Global climate change means such freezing events are becoming less predictable. This in turn reduces the ability of plants to predict the approaching low temperatures and cold acclimate. This has consequences for crop yields and distribution of wild plant species. C-repeat binding factors (CBFs) are transcription factors previously shown to play a vital role in the acclimation process of Arabidopsis thaliana, controlling the expression of hundreds of genes whose products are necessary for freezing tolerance. Work in other plant species cements CBFs as key determinants in the trait of freezing tolerance in higher plants. To test the function of CBFs from highly freezing tolerant plants species we cloned and sequenced CBF transcription factors from three Vaccinium species (Vaccinium myrtillus, Vaccinium uliginosum and Vaccinium vitis-idaea) which we collected in the Arctic. We tested the activity of CBF transcription factors from the three Vaccinium species by producing transgenic Arabidopsis lines overexpressing them. Only the Vaccinium myrtillus CBF was able to substantially activate COR (CBF-target) gene expression in the absence of cold. Correspondingly, only the lines expressing the Vaccinium myrtillus CBF were constitutively freezing tolerant. The basis for the differences in potency of the three Vaccinium CBFs was tested by observing cellular localisation and protein levels. All three CBFs were correctly targeted to the nucleus, but Vaccinium uliginosum CBF appeared to be relatively unstable. The reasons for lack of potency for Vaccinium vitis-idaea CBF were not due to stability or targeting, and we speculate that this was due to altered transcription factor function.
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a c repeat binding factor transcriptional activator cbf dreb1 from european bilberry Vaccinium myrtillus induces freezing tolerance when expressed in arabidopsis thaliana
PLOS ONE, 2013Co-Authors: Rachael J. Oakenfull, Robert Baxter, Marc R. KnightAbstract:Freezing stress affects all plants from temperate zones to the poles. Global climate change means such freezing events are becoming less predictable. This in turn reduces the ability of plants to predict the approaching low temperatures and cold acclimate. This has consequences for crop yields and distribution of wild plant species. C-repeat binding factors (CBFs) are transcription factors previously shown to play a vital role in the acclimation process of Arabidopsis thaliana, controlling the expression of hundreds of genes whose products are necessary for freezing tolerance. Work in other plant species cements CBFs as key determinants in the trait of freezing tolerance in higher plants. To test the function of CBFs from highly freezing tolerant plants species we cloned and sequenced CBF transcription factors from three Vaccinium species (Vaccinium myrtillus, Vaccinium uliginosum and Vaccinium vitis-idaea) which we collected in the Arctic. We tested the activity of CBF transcription factors from the three Vaccinium species by producing transgenic Arabidopsis lines overexpressing them. Only the Vaccinium myrtillus CBF was able to substantially activate COR (CBF-target) gene expression in the absence of cold. Correspondingly, only the lines expressing the Vaccinium myrtillus CBF were constitutively freezing tolerant. The basis for the differences in potency of the three Vaccinium CBFs was tested by observing cellular localisation and protein levels. All three CBFs were correctly targeted to the nucleus, but Vaccinium uliginosum CBF appeared to be relatively unstable. The reasons for lack of potency for Vaccinium vitis-idaea CBF were not due to stability or targeting, and we speculate that this was due to altered transcription factor function.
Robert Baxter - One of the best experts on this subject based on the ideXlab platform.
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A C-repeat binding factor transcriptional activator (CBF/DREB1) from European bilberry (Vaccinium myrtillus) induces freezing tolerance when expressed in Arabidopsis thaliana.
PloS one, 2013Co-Authors: Rachael J. Oakenfull, Robert Baxter, Marc R. KnightAbstract:Freezing stress affects all plants from temperate zones to the poles. Global climate change means such freezing events are becoming less predictable. This in turn reduces the ability of plants to predict the approaching low temperatures and cold acclimate. This has consequences for crop yields and distribution of wild plant species. C-repeat binding factors (CBFs) are transcription factors previously shown to play a vital role in the acclimation process of Arabidopsis thaliana, controlling the expression of hundreds of genes whose products are necessary for freezing tolerance. Work in other plant species cements CBFs as key determinants in the trait of freezing tolerance in higher plants. To test the function of CBFs from highly freezing tolerant plants species we cloned and sequenced CBF transcription factors from three Vaccinium species (Vaccinium myrtillus, Vaccinium uliginosum and Vaccinium vitis-idaea) which we collected in the Arctic. We tested the activity of CBF transcription factors from the three Vaccinium species by producing transgenic Arabidopsis lines overexpressing them. Only the Vaccinium myrtillus CBF was able to substantially activate COR (CBF-target) gene expression in the absence of cold. Correspondingly, only the lines expressing the Vaccinium myrtillus CBF were constitutively freezing tolerant. The basis for the differences in potency of the three Vaccinium CBFs was tested by observing cellular localisation and protein levels. All three CBFs were correctly targeted to the nucleus, but Vaccinium uliginosum CBF appeared to be relatively unstable. The reasons for lack of potency for Vaccinium vitis-idaea CBF were not due to stability or targeting, and we speculate that this was due to altered transcription factor function.
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a c repeat binding factor transcriptional activator cbf dreb1 from european bilberry Vaccinium myrtillus induces freezing tolerance when expressed in arabidopsis thaliana
PLOS ONE, 2013Co-Authors: Rachael J. Oakenfull, Robert Baxter, Marc R. KnightAbstract:Freezing stress affects all plants from temperate zones to the poles. Global climate change means such freezing events are becoming less predictable. This in turn reduces the ability of plants to predict the approaching low temperatures and cold acclimate. This has consequences for crop yields and distribution of wild plant species. C-repeat binding factors (CBFs) are transcription factors previously shown to play a vital role in the acclimation process of Arabidopsis thaliana, controlling the expression of hundreds of genes whose products are necessary for freezing tolerance. Work in other plant species cements CBFs as key determinants in the trait of freezing tolerance in higher plants. To test the function of CBFs from highly freezing tolerant plants species we cloned and sequenced CBF transcription factors from three Vaccinium species (Vaccinium myrtillus, Vaccinium uliginosum and Vaccinium vitis-idaea) which we collected in the Arctic. We tested the activity of CBF transcription factors from the three Vaccinium species by producing transgenic Arabidopsis lines overexpressing them. Only the Vaccinium myrtillus CBF was able to substantially activate COR (CBF-target) gene expression in the absence of cold. Correspondingly, only the lines expressing the Vaccinium myrtillus CBF were constitutively freezing tolerant. The basis for the differences in potency of the three Vaccinium CBFs was tested by observing cellular localisation and protein levels. All three CBFs were correctly targeted to the nucleus, but Vaccinium uliginosum CBF appeared to be relatively unstable. The reasons for lack of potency for Vaccinium vitis-idaea CBF were not due to stability or targeting, and we speculate that this was due to altered transcription factor function.
Baoru Yang - One of the best experts on this subject based on the ideXlab platform.
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Characterization and Quantification of Nonanthocyanin Phenolic Compounds in White and Blue Bilberry (Vaccinium myrtillus) Juices and Wines Using UHPLC-DAD-ESI-QTOF-MS and UHPLC-DAD.
Journal of agricultural and food chemistry, 2020Co-Authors: Shuxun Liu, Alexis Marsol-vall, Oskar Laaksonen, Maaria Kortesniemi, Baoru YangAbstract:The non-anthocyanin phenolic compounds in juice and wine produced from fruits of white bilberry, a non-pigmented mutant of Vaccinium myrtillus, and blue bilberry (pigmented variety) were analyzed u...
Laura Jaakola - One of the best experts on this subject based on the ideXlab platform.
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Changes in the Proanthocyanidin Composition and Related Gene Expression in Bilberry (Vaccinium myrtillus L.) Tissues
Journal of agricultural and food chemistry, 2020Co-Authors: Jussi Suvanto, Laura Jaakola, Katja Karppinen, Kaisu Riihinen, Juha-pekka SalminenAbstract:Berries of genus Vaccinium are rich in flavonoids and proanthocyanidins (PAs). We studied the PA composition and biosynthesis in bilberry (Vaccinium myrtillus L.) tissues and during fruit developme...
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Bilberry (Vaccinium myrtillus L.) Ecotypes
Nutritional Composition of Fruit Cultivars, 2016Co-Authors: Laura Zoratti, Hannele Klemettilä, Laura JaakolaAbstract:Abstract Bilberry (Vaccinium myrtillus L.) is an important wild berry that has long tradition as a food and medicinal plant in Europe. Nowadays, bilberries are picked commercially, especially in northern and eastern parts of Europe. These berries are among the best sources of anthocyanin pigments, which have multiple health-beneficial properties. In addition to anthocyanins, bilberries also contain high yields other phenolic compounds and carotenoids, and are a good-to-moderate source of vitamins and both macro- and micro-nutrients. Variation in the quantitative and qualitative composition of phytonutrients has been detected between different bilberry ecotypes, but more comprehensive studies on this topic are still needed. As bilberries have not been subjected to breeding or cultivation programs, the populations of this species still possess the natural variation developed as a result of long-term adaptation, under different growth conditions.
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Anthocyanin fingerprinting for authenticity studies of bilberry (Vaccinium myrtillus L.)
Food Control, 2013Co-Authors: Anja K. Primetta, Laura Jaakola, Faik Ahmet Ayaz, Huseyin Inceer, Kaisu RiihinenAbstract:Abstract The high valued bilberries of Vaccinium myrtillus L. and the products derived from this berry have been mislabelled and adulterated with substitutes such as synthetic chemical or cheaper berries. Anthocyanin profiles have been successfully used in quality control as all berries have their own typical anthocyanin profile. However, in the case of wild species, the natural variation caused by genetic and environmental differences has to be investigated in order to establish a valid anthocyanin fingerprint. This is the first study to analyse the anthocyanin (aglycone and sugar moiety) profiles of bilberries originating from ten populations from climatically diverse regions of Asian Turkey. We compare the anthocyanin profiles of Turkish bilberries with their previously studied northern European Finnish counterparts with the same optimized RP-HPLC-DAD method.The Turkish bilberries did not significantly differ from their Finnish counterparts in the aglycones, which may be considered as a consistent feature for the identification of V. myrtillus. The results also showed that the sugar moieties were grouped according to the origin. A logistic regression model based on glucoside proportions classified 96.7% of samples correctly into their geographical region of origin.This may be used as a novel discriminating criteria for distinguishing bilberries among different geographical origins. The results demonstrate a basis for a new model and provide information for quality control, label claim verification and raw material source identification of V. myrtillus and related species for industry and regulatory authorities.
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Ascorbic acid metabolism during bilberry (Vaccinium myrtillus L.) fruit development.
Journal of plant physiology, 2012Co-Authors: Giacomo Cocetta, Katja Karppinen, Marko Suokas, Anja Hohtola, Hely Häggman, Anna Spinardi, Ilaria Mignani, Laura JaakolaAbstract:Bilberry (Vaccinium myrtillus L.) possesses a high antioxidant capacity in berries due to the presence of anthocyanins and ascorbic acid (AsA). Accumulation of AsA and the expression of the genes encoding the enzymes of the main AsA biosynthetic route and of the ascorbate–glutathione cycle, as well as the activities of the enzymes involved in AsA oxidation and recycling were investigated for the first time during the development and ripening of bilberry fruit. The results showed that the AsA level remained relatively stable during fruit maturation. The expression of the genes encoding the key enzymes in the AsA main biosynthetic route showed consistent trends with each other as well as with AsA levels, especially during the first stages of fruit ripening. The expression of genes and activities of the enzyme involved in the AsA oxidation and recycling route showed more prominent developmental stage-dependent changes during the ripening process. Different patterns of activity were found among the studied enzymes and the results were, for some enzymes, in accordance with AsA levels. In fully ripe berries, both AsA content and gene expression were significantly higher in skin than in pulp.