Vitis Amurensis

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Jianmin Tao - One of the best experts on this subject based on the ideXlab platform.

  • Stress-responsive gene ICE1 from Vitis Amurensis increases cold tolerance in tobacco
    Plant physiology and biochemistry : PPB, 2013
    Co-Authors: Chang Dong, Zhen Zhang, Junpeng Ren, Yang Qin, Jinfeng Huang, Yan Wang, Binhua Cai, Bailin Wang, Jianmin Tao
    Abstract:

    We report the identification of the inducer of CBF expression 1 (ICE1) from Vitis Amurensis, an upstream transcription factor that regulates the transcription of CBF-like genes. The structure of the basic helix-loop-helix domain of VaICE1 is closely related to that of ICE1 in woody plants. This gene is strongly induced in leaves, roots, stems, and petioles by cold temperature. With longer duration of exposure to cold treatments, the expression patterns of organs exhibit differences, which are not observed in normal condition. Transgenic tobacco over-expressing VaICE1 has higher chilling tolerance and survival ability by improving the activities of superoxide dismutase, peroxidase, and catalase, as well as the chlorophyll yield.

  • VaCBF1 from Vitis Amurensis associated with cold acclimation and cold tolerance
    Acta Physiologiae Plantarum, 2013
    Co-Authors: Chang Dong, Zhen Zhang, Junpeng Ren, Yang Qin, Jinfeng Huang, Binhua Cai, Bailin Wang, Lu Huiling, Jianmin Tao
    Abstract:

    To understand cold acclimation and cold tolerance in Vitis, we isolated a C-repeat binding factor 1 (CBF1) transcriptional activator from cold-sensitive Vitis vinifera ‘Manicure Finger’ and cold-tolerant wild Vitis Amurensis. Under cold stress, the CBF1 transcript accumulation of V. Amurensis increased, whereas that of V. vinifera showed no significant change. The transcript levels of VaCBF1 in the roots, stems, leaves, and petioles under cold stress were up-regulated in a time-dependent manner. The transcript level of VaCBF1 in the leaves was induced by salinity stress or by exogenous abscisic acid and salicylic acid. The presence of the cis-elements MBS, MYB, and MYC in the VaCBF1 promoter suggests that this promoter is a component of the CBF transduction pathway, which is involved in plant response to cold stress. The overexpression of VaCBF1 increased the cold tolerance of transgenic tobacco at −4 °C. The transcript level of the downstream target gene NtERD10D appeared in the transgenic lines under normal conditions, whereas that of NtERD10D and NtDREB3 improved under low temperature. We suggest that VaCBF1 enhances stress tolerance by increasing antioxidant activities and promoting downstream target gene expression.

  • Isolation and expression analysis of CBF4 from Vitis Amurensis associated with stress
    Agricultural Sciences, 2013
    Co-Authors: Chang Dong, Zhen Zhang, Junpeng Ren, Yang Qin, Bailin Wang, Meng Zhang, Xiao Lizhen, Jianmin Tao
    Abstract:

    The C-repeat Binding Factor/Dehydration Responsive Element Binding factor (CBF/DREB) responsive pathway is involved in plant response to abiotic stress. In this study, we report the isolation of VaCBF4 (a complete cDNA) and its promoter from Vitis Amurensis through rapid amplification of cDNA ends and genome walking techniques, respectively. The CBF4 transcript accumulation of V. Amurensis increased under cold, salinity, and abscisic acid (ABA) and salicylic acid (SA) treatments, whereas that of Vitis vinifera showed a different change. The transcript levels of VaCBF4 inthe roots, stems, leaves, and petioles under cold, salinity, and ABA and SA treatments were up-regulated in a timedependent manner. The presence of the cis-elements MBC, ABRE, and as-2-box in the VaCBF4 promoter further confirmed that this promoter is a component of the CBF transduction pathway, which is involved in plant response to multistress.

  • Isolation and expression characterization of CBF2 in Vitis Amurensis with stress
    Agricultural Sciences, 2013
    Co-Authors: Chang Dong, Yang Qin, Binhua Cai, Bailin Wang, Jianmin Tao, Meng Zhang, Zhen Zhang
    Abstract:

    The transcription factor VaCBF2, which interacts with C-repeat/DRE and its promoter, was isolated from Vitis Amurensis. The VaCBF2 amino acid sequence contained a conserved AP2 domain of 56 amino acids and a potential nuclear localization sequence. The sequence of VaCBF2 showed a high level of homology with other CBF2 family members. Phylogenetic analysis showed that the amino acid sequences may be CBF2 proteins with evolutionary relationship. Quantitative reverse-transcription polymerase chain reaction analysis indicated that the expression of VaCBF2 gene in tissues (roots, stems, leaves, and petioles) was induced by low temperature, high salinity, and application of abscisic acid and salicylic acid in a time-dependent manner but to different extents in the cold-hardy V. Amurensis and the less cold-hardy Vitis vinifera. The presence of cis-elements such as MYC and ABRE in VaCBF2 promoter further confirmed that this promoter was a component of the CBF transduction pathway involved in plant response to multiple stresses.

Shen Yu-jie - One of the best experts on this subject based on the ideXlab platform.

  • Effects of Exogenous Nitric Oxide on Chlorophyll Fluorescence and Antioxidant Enzymes Activity in Amur Grape (Vitis Amurensis Rupr) Leaves under Salt Stress
    Journal of Nuclear Agricultural Sciences, 2013
    Co-Authors: Shen Yu-jie
    Abstract:

    To evaluate the role of nitric oxide(NO) in plant salt tolerance,this study was conducted in nutrient solution to investigate the effects of exogenous sodium nitroprusside(SNP),an NO donor,on chlorophyll content,chlorophyll fluorescence parameters and antioxidant enzymes activity in Vitis Amurensis 'Shuangfeng' 1-year cutting seedling under 50 mmol·L-1 NaCl stress.The results showed that exogenous NO significantly promoted the activity of superoxide dismutase(SOD) and ascorbate peroxidase(APX) in Vitis Amurensis leavs,which led to the decrease of malondialdehyde(MDA),thus protected chloroplast memberance stability and increased chlorophyll content.On the other hand,exogenous NO increased photochemical efficiency of photosystem II(Fv/Fm),actual photochemical efficiency(ΦPSII),photochemical quenching coefficient(qP) and regulated thermal energy dissipation(ΦNPQ) under NaCl stress.However,the minimal fluorescence(Fo) and decreased photo damage(ΦNO) in Vitis Amurensis leaves were decreased.These results indicated that NO enhanced the tolerance of Vitis Amurensis seedlings to NaCl stress through the protecting mechanism of thermal dissipation which alleviated photo inhibition caused by salt stress,improving photochemical electron transport efficiency and maintaining the normal function of PSII to some extent.

  • The Distribution and Dynamic Changes of Procyanidin Contents in Vitis Amurensis Germplasm Resources
    Northern Horticulture, 2011
    Co-Authors: Shen Yu-jie
    Abstract:

    The contents of procyanidin were detected by Folin-Ciocalteu method in combination with HPLC.The characteristics of distribution and contents of procyanidin in 39 Vitis Amurensis germplasm resources and 'Beta' were determined to reveal the differences and characteristics among different germplasm resources.And dynamic changes of procyanidin contents in leaves,stem bark,peel and seed of 4 Vitis Amurensis varieties 'Shuangyou','Shuanghong', 'Siangfeng','Shuangqing' were studied to reveal the law of changes of the procyanidin contents in different organs.The results showed that the contents of procyanidin in seeds and peel varied greatly,the value ranged from 0.42~8 mg/g FW and 1.2~14.7 mg/g FW,respectively.The difference of contents of procyanidin was not significant among different flowers sex germplasm,among different regions germplasm.The contents of anthocyanidins in Peel were significantly greater than seeds.The dynamic changes characteristics of Procyanidin contents in different organs of 4 main cultivars: The contents of anthocyanins in leaves increased in young fruiting period,and declined in turning-color period,and increased slightly in maturing stage;The contents of anthocyanins in stem bark increased,and declined in turning-color period and maturing stage;The contents of anthocyanins in peel increased slowly in young fruiting period and turning-color period,and increased rapidly in maturing stage;It increased in young fruiting period and hading period,and declined in maturing stage.

  • Present Research Status and Countermeasures of Downy Mildew in Vitis Amurensis Rupr.
    Northern Horticulture, 2010
    Co-Authors: Shen Yu-jie
    Abstract:

    Downy mildew is one of most serious diseases in Vitis Amurensis Rupr..With the expansion of cultivated area,the hazard of downy mildew increasing year by year,and directly affect the grape fruit quality and yield which used for wine-making.In China,downy mildew began to spread in the production regions of Vitis Amurensis Rupr.In late June,and into the peak till early August.Researchers had not yet found immune varieties or types and there was no pesticide to eradicate the disease.Authors want to discuss some problems within the downy mildew occurrence,damage,present research situations and protection,and made countermeasures to how to research and control the downy mildew.

  • Study on Improving Fruit Setting Rate of Vitis Amurensis Rupr
    Special Wild Economic Animal and Plant Research, 2009
    Co-Authors: Shen Yu-jie
    Abstract:

    An experiment conducted for increasing fruit-setting of the new breed "BeiBingHong" and the new strains 94-7-75、 98-8-165、2001-6-135 indicated:PP333 1 000mg/L + 0.3% boric acid aqueous solution,0.3% boric acid aqueous solution,male pollens of Vitis Amurensis 25g+0.3% boric acid+0.5% urea aqueous solution and 0.3% boric acid+0.5% sugar solution could increase the fruit setting rate significantly and increased respectively by 11.3%,12.2%,14.1% and 16.1% compared to the control.Effect of 0.3% boric acid + 0.5% sugar solution was the best.The treatment would help to increase yield and was suggested to promote application in production.

  • Industrial Development and Countermeasure Vitis Amurensis Rupr in China
    2002
    Co-Authors: Shen Yu-jie
    Abstract:

    productive current status and existing problems were maiorly summed up in productive cultivation and wine ofVitis AmurensisRupr in this paper And countermeasure were also put forward about improving the continuous industrialdevelopment of Vitis Amurensis Rupr.

Feng Yu-cai - One of the best experts on this subject based on the ideXlab platform.

  • Effect of Saline-Alkali Stress on Photosynthesis Characteristic of Vitis Amurensis Rupr
    Northern Horticulture, 2008
    Co-Authors: Feng Yu-cai
    Abstract:

    3 cultivars of Vitis Amurensis Rupr were selected,effect of saline-alkali Stress on photosynthesis、transpiration rate and stomatal resistance were studied.The result indicated that:Saline-Alkali Stress has obvious inhibition on all of three,and transpiration rate and stomatal resistance of different cultivar were different under the same treatment;transpiration rate and stomatal resistance of a cultivar were different under the different treatment.

  • The Harm of Saline-Alkali Stress on Vitis Amurensis Rupr
    Northern Horticulture, 2007
    Co-Authors: Feng Yu-cai
    Abstract:

    Studied survival rates of plant、harm ratio、appear time and grade of harm of 3 cultivars of Vitis Amurensis Rupr under saline-alkali stress,then the tolerance to salt and alkaline of different cultivars were concluded.The results indicated that: "Gong-Ning I" cultivar,which has the highest tolerance to salt and alkaline,"Zuo Shan I"was secondary,"Hong Bao Shi" was the lowest.

Chang Dong - One of the best experts on this subject based on the ideXlab platform.

  • Stress-responsive gene ICE1 from Vitis Amurensis increases cold tolerance in tobacco
    Plant physiology and biochemistry : PPB, 2013
    Co-Authors: Chang Dong, Zhen Zhang, Junpeng Ren, Yang Qin, Jinfeng Huang, Yan Wang, Binhua Cai, Bailin Wang, Jianmin Tao
    Abstract:

    We report the identification of the inducer of CBF expression 1 (ICE1) from Vitis Amurensis, an upstream transcription factor that regulates the transcription of CBF-like genes. The structure of the basic helix-loop-helix domain of VaICE1 is closely related to that of ICE1 in woody plants. This gene is strongly induced in leaves, roots, stems, and petioles by cold temperature. With longer duration of exposure to cold treatments, the expression patterns of organs exhibit differences, which are not observed in normal condition. Transgenic tobacco over-expressing VaICE1 has higher chilling tolerance and survival ability by improving the activities of superoxide dismutase, peroxidase, and catalase, as well as the chlorophyll yield.

  • VaCBF1 from Vitis Amurensis associated with cold acclimation and cold tolerance
    Acta Physiologiae Plantarum, 2013
    Co-Authors: Chang Dong, Zhen Zhang, Junpeng Ren, Yang Qin, Jinfeng Huang, Binhua Cai, Bailin Wang, Lu Huiling, Jianmin Tao
    Abstract:

    To understand cold acclimation and cold tolerance in Vitis, we isolated a C-repeat binding factor 1 (CBF1) transcriptional activator from cold-sensitive Vitis vinifera ‘Manicure Finger’ and cold-tolerant wild Vitis Amurensis. Under cold stress, the CBF1 transcript accumulation of V. Amurensis increased, whereas that of V. vinifera showed no significant change. The transcript levels of VaCBF1 in the roots, stems, leaves, and petioles under cold stress were up-regulated in a time-dependent manner. The transcript level of VaCBF1 in the leaves was induced by salinity stress or by exogenous abscisic acid and salicylic acid. The presence of the cis-elements MBS, MYB, and MYC in the VaCBF1 promoter suggests that this promoter is a component of the CBF transduction pathway, which is involved in plant response to cold stress. The overexpression of VaCBF1 increased the cold tolerance of transgenic tobacco at −4 °C. The transcript level of the downstream target gene NtERD10D appeared in the transgenic lines under normal conditions, whereas that of NtERD10D and NtDREB3 improved under low temperature. We suggest that VaCBF1 enhances stress tolerance by increasing antioxidant activities and promoting downstream target gene expression.

  • Isolation and expression analysis of CBF4 from Vitis Amurensis associated with stress
    Agricultural Sciences, 2013
    Co-Authors: Chang Dong, Zhen Zhang, Junpeng Ren, Yang Qin, Bailin Wang, Meng Zhang, Xiao Lizhen, Jianmin Tao
    Abstract:

    The C-repeat Binding Factor/Dehydration Responsive Element Binding factor (CBF/DREB) responsive pathway is involved in plant response to abiotic stress. In this study, we report the isolation of VaCBF4 (a complete cDNA) and its promoter from Vitis Amurensis through rapid amplification of cDNA ends and genome walking techniques, respectively. The CBF4 transcript accumulation of V. Amurensis increased under cold, salinity, and abscisic acid (ABA) and salicylic acid (SA) treatments, whereas that of Vitis vinifera showed a different change. The transcript levels of VaCBF4 inthe roots, stems, leaves, and petioles under cold, salinity, and ABA and SA treatments were up-regulated in a timedependent manner. The presence of the cis-elements MBC, ABRE, and as-2-box in the VaCBF4 promoter further confirmed that this promoter is a component of the CBF transduction pathway, which is involved in plant response to multistress.

  • Isolation and expression characterization of CBF2 in Vitis Amurensis with stress
    Agricultural Sciences, 2013
    Co-Authors: Chang Dong, Yang Qin, Binhua Cai, Bailin Wang, Jianmin Tao, Meng Zhang, Zhen Zhang
    Abstract:

    The transcription factor VaCBF2, which interacts with C-repeat/DRE and its promoter, was isolated from Vitis Amurensis. The VaCBF2 amino acid sequence contained a conserved AP2 domain of 56 amino acids and a potential nuclear localization sequence. The sequence of VaCBF2 showed a high level of homology with other CBF2 family members. Phylogenetic analysis showed that the amino acid sequences may be CBF2 proteins with evolutionary relationship. Quantitative reverse-transcription polymerase chain reaction analysis indicated that the expression of VaCBF2 gene in tissues (roots, stems, leaves, and petioles) was induced by low temperature, high salinity, and application of abscisic acid and salicylic acid in a time-dependent manner but to different extents in the cold-hardy V. Amurensis and the less cold-hardy Vitis vinifera. The presence of cis-elements such as MYC and ABRE in VaCBF2 promoter further confirmed that this promoter was a component of the CBF transduction pathway involved in plant response to multiple stresses.

Wang Ming - One of the best experts on this subject based on the ideXlab platform.

  • Studies on Resveratrol Content in Vitis Amurensis from Different Areas of Jilin Province
    Journal of Jilin Agricultural University, 2010
    Co-Authors: Wang Ming, Qin Lei, He Yuping
    Abstract:

    Resveratrol content in Vitis Amurensis Rupr.of different areas,such as Ji'an,Zuojia,Songyuan,Changchun,was determined with high pressure liquid chromatography(HPLC).The results showed that the berries of Shuangyou and Shuanghong in Songyuan had the highest resveratrol content than in other areas due to its great diffenence in temperature during day and night.Gongniang No.1 had the highest resveratrol content than other varieties in Ji'an.When in Songyuan,Shuanghong had the highest content than other varieties.

  • Effect of Saline-Alkali Stress on the Physiological Characteristics of Vitis Amurensis
    Journal of Jilin Agricultural University, 2008
    Co-Authors: Wang Ming
    Abstract:

    Physiological characteristics of Vitis Amurensis(Zuo shanyi,Shuanghong,Gongniangyihao) under salt stress were studied.The results showed that saline-alkali stress promoted content of proline,increased membrane permeability,and promoted activity of nitrate reductase.

  • Changes of Respiration Intensity and Main Nutritional Components of Fruit of Vitis Amurensis during Maturation
    Journal of Jilin Agricultural University, 2001
    Co-Authors: L. H. Iyan, Wang Ming, Feng Yucai, Dong Shi-liang, Huangfu Chun, Liu Hongzhang
    Abstract:

    Shuangyou”and“Zuoshanyi”, two cultivars of wild grape ( Vitis Amurensis Rupr ) were used in the determinations of respiration intensity and main nutritional components in the fruits during maturation. The results obtained were as follows: The respiration increased at the early stage and decreased later, or decreased all through the maturation. The 100 berries weight increased during the early maturation and decreased later. The contents of total sugar, vitamin C, pigment, and total free amino acids increased; while the contents of acid and tannin decreased during maturation.