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Pablo Castillo - One of the best experts on this subject based on the ideXlab platform.
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Integrative taxonomy of Xiphinema histriae and Xiphinema lapidosum from Spain
Journal of Nematology, 2019Co-Authors: Antonio Archidona-yuste, Carolina Cantalapiedra-navarrete, Juan E. Palomares-rius, Jingwu Zheng, Pablo CastilloAbstract:Three populations of Xiphinema non-americanum group species were detected in agricultural and natural ecosystems, during routine surveys for plant-parasitic nematodes in Spain. Based on morphological and molecular analyses, the species were identified as Xiphinema histriae and Xiphinema lapidosum, being this the first record and molecular characterization of both species in Spain. The morphometrics and morphology of the Spanish populations agree with those of the original description and paratype specimens and the present study provided a first description of the second to fourth juvenile stages of both species. A detailed study on the morphology in the Spanish populations of X. histriae, as well as in paratypes, showed a pseudo-Z-organ with weakly muscularized wall and containing numerous small dense granular bodies, which was different to the original description by Lamberti et al. (1993). This new finding suggests that X. histriae must be considered a member of the morphospecies Group 5 of X. non-americanum. Phylogenetic analysis based on D2 to D3 expansion segments of 28S gene, ITS1 and partial CoxI gene indicated that X. histriae and X. lapidosum are phylogenetically related with other Xiphinema non-americanum group spp. reported from Spain. Considering the pathological and economic importance of this group of nematodes, the combination of morphological characters, measurements, and molecular analysis is crucial for accurate identification of these species.
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a new dagger nematode Xiphinema poasense n sp nematoda longidoridae from costa rica
Nematology, 2018Co-Authors: Ingrid Varelabenavides, Carolina Cantalapiedranavarrete, Pablo Castillo, Juan E Palomaresrius, Walter Perazapadilla, Antonio ArchidonayusteAbstract:A new dagger nematode, Xiphinema poasense n. sp., is described and illustrated from three populations extracted from soil associated with a combined plantation of Eucalyptus sp., Cupressus sp. and Pennisetum sp. and wild plants from a tropical pre-montane forest in Costa Rica. The new dagger nematode is characterised by a moderate body size 2612 (2416-3042) μ m long, a rounded lip region 15.0 (13.5-16.5) μ m broad, separated from the body contour by a shallow depression, amphidial fovea large, stirrup-shaped, a very long odontostyle (175 (164-188) μ m), stylet guiding ring located 167 (136-181) μ m from anterior end, vulva situated anterior to mid-body (36-40%), anterior genital branch complete but strongly reduced, without uterine differentiation, female tail short, hemispherical to convex-conoid with a c′ ratio = 0.7 (0.6-0.8) and bearing two pairs of caudal pores, and male absent. Integrative diagnosis was completed with molecular data using D2-D3 expansion segments of 28S rRNA, ITS1 region, partial 18S-rRNA and the partial mitochondrial gene cytochrome c oxidase subunit 1 ( coxI ). The phylogenetic relationships based on D2-D3 segments of this species with other Xiphinema spp. of the X. non- americanum group indicated that X. poasense n. sp. clustered with other species with a reduced anterior genital branch from the morphospecies Group 2, viz. , X. costaricense and X. krugi . However, the phylogeny of coxI and partial 18S rRNA gene revealed that the new species did not cluster with Xiphinema species having the anterior genital branch absent or reduced ( i.e. , morphospecies Groups 1 and 2, respectively).
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a new dagger nematode Xiphinema tica n sp nematoda longidoridae from costa rica with updating of the polytomous key of loof and luc 1990
European Journal of Plant Pathology, 2018Co-Authors: Walter Perazapadilla, Carolina Cantalapiedranavarrete, Pablo Castillo, Juan E Palomaresrius, Tatiana Zamoraaraya, Antonio ArchidonayusteAbstract:A new dagger nematode, Xiphinema tica n. sp., is described and illustrated from several populations extracted from soil associated with several crops and wild plants in Costa Rica. The new dagger nematode is characterised by a moderate body size (3276–4240 μm), a rounded lip region, ca 13.5 μm wide, separated from body contour by a shallow depression, amphidial fovea large, stirrup-shaped, a moderately long odontostyle ca 135 μm long, stylet guiding ring located at ca 122 μm from anterior end, vulva almost equatorial (50–54%), well-developed Z-organ, with heavy muscularised wall containing in the most of specimens observed two moderately refractive inclusions variable in shape (from round to star-shaped), with uterine spines and crystalloid bodies; female tail short, dorsally convex-conoid, with rounded end and a small peg, with a c’ ratio ca 0.8, bearing two or three pairs of caudal pores and male absent. The unique and novel uterine differentiation based on the coexistence of a well-developed Z-organ mixed with uterine spines and crystalloid bodies in Xiphinema prompted us to update and include this combination of characters in the polytomous key of Loof and Luc (1990). Integrative diagnosis was completed with molecular data obtained, using D2-D3 expansion segments of 28S rDNA, ITS1-rDNA, partial 18S–rDNA and the partial mitochondrial gene cytochrome c oxidase subunit 1 (coxI). The phylogenetic relationships of this species with other Xiphinema spp. indicated that X. tica n. sp. was monophyletic to the other species from the morphospecies Group 4, Xiphinema oleae.
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molecular characterization of pseudomonodelphic dagger nematodes of the genus Xiphinema cobb 1913 nematoda longidoridae in costa rica with notes on Xiphinema setariae tarjan 1964
European Journal of Plant Pathology, 2017Co-Authors: Walter Perazapadilla, Carolina Cantalapiedranavarrete, Antonio Archidonayuste, Juan E Palomaresrius, H Ferris, Tatiana Zamoraaraya, Sergei A Subbotin, Pablo CastilloAbstract:Pseudomonodelphic dagger nematodes of the genus Xiphinema are characterized by having one of the genital branches reduced and lacking an ovary. They are usually reported from tropical regions. Nematode surveys conducted during rainy seasons in Costa Rica resulted in detection of several Xiphinema costaricense populations, but also other pseudomonodelphic and didelphic species of Xiphinema. We undertook detailed integrative morphometric and molecular studies using D2-D3 expansion segments of 28S rDNA, and ITS1-rDNA. From those studies, we also identified several populations of Xiphinema krugi and two populations of Xiphinema setariae with characteristics in agreement with those of the original and later descriptions of these species. The phylogenetic analyses of these species with other representatives of Xiphinema spp. indicated that pseudomonodelphic species are phylogenetically related (X. costaricense and X. krugi). On the basis of ITS1 sequences of X. costaricense and X. variegatum from Brazil, as well as similar morphology and morphometrics of both species, the latter is proposed here as a junior synonym of the former. Also, our morphometric data showed some intraspecific variability within X. setariae, which in combination with the molecular evidence, suggests that X. setariae and X. vulgare need to be considered as a single taxon.
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molecular phylogenetic analysis and comparative morphology resolve two new species of olive tree soil related dagger nematodes of the genus Xiphinema dorylaimida longidoridae from spain
Invertebrate Systematics, 2016Co-Authors: Antonio Archidonayuste, Carolina Cantalapiedranavarrete, Juan A Navascortes, Juan E Palomaresrius, Pablo CastilloAbstract:The genus Xiphinema constitutes a large group of ~275 species of polyphagous, plant-ectoparasitic nematodes that are distributed almost worldwide. Some species affect agricultural crops by feeding directly on root cells as well as by transmitting nepoviruses. Despite their agricultural importance, species discrimination in Xiphinema is difficult, leading to potential misidentification. Integrative taxonomy, based on the combination of molecular analyses and morphology, constitutes a new insight into Xiphinema species identification. In this study we describe two new species of Xiphinema from the Iberian Peninsula (X. macrodora, sp. nov. and X. oleae, sp. nov.) associated with cultivated and wild olive trees. Both species have specific rRNA sequences. Morphologically, Xiphinema macrodora, sp. nov. is characterised by a very long body (7.2–8.7 mm), a very long odontostyle and odontophore (190–206 and 105–120 µm, respectively), and a well developed pseudo-Z-organ, comprising 8 to 12 sclerotised bodies. Xiphinema oleae, sp. nov. is characterised by an odontostyle and an odontophore 136–149 and 65–80 µm long, respectively, and a well developed Z-organ with refractive inclusions (3–5), variable in shape. Additionally, X. macrodora, sp. nov. has the longest body size, and the longest odontostyle and odontophore of any Xiphinema, whereas X. oleae, sp. nov. is the first species with a well developed Z-organ from the Iberian Peninsula. http://zoobank.org/urn:lsid:zoobank.org:pub:E4CE8711-22EF-40E4-9D39-6FE962F25225
Carolina Cantalapiedranavarrete - One of the best experts on this subject based on the ideXlab platform.
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a new dagger nematode Xiphinema poasense n sp nematoda longidoridae from costa rica
Nematology, 2018Co-Authors: Ingrid Varelabenavides, Carolina Cantalapiedranavarrete, Pablo Castillo, Juan E Palomaresrius, Walter Perazapadilla, Antonio ArchidonayusteAbstract:A new dagger nematode, Xiphinema poasense n. sp., is described and illustrated from three populations extracted from soil associated with a combined plantation of Eucalyptus sp., Cupressus sp. and Pennisetum sp. and wild plants from a tropical pre-montane forest in Costa Rica. The new dagger nematode is characterised by a moderate body size 2612 (2416-3042) μ m long, a rounded lip region 15.0 (13.5-16.5) μ m broad, separated from the body contour by a shallow depression, amphidial fovea large, stirrup-shaped, a very long odontostyle (175 (164-188) μ m), stylet guiding ring located 167 (136-181) μ m from anterior end, vulva situated anterior to mid-body (36-40%), anterior genital branch complete but strongly reduced, without uterine differentiation, female tail short, hemispherical to convex-conoid with a c′ ratio = 0.7 (0.6-0.8) and bearing two pairs of caudal pores, and male absent. Integrative diagnosis was completed with molecular data using D2-D3 expansion segments of 28S rRNA, ITS1 region, partial 18S-rRNA and the partial mitochondrial gene cytochrome c oxidase subunit 1 ( coxI ). The phylogenetic relationships based on D2-D3 segments of this species with other Xiphinema spp. of the X. non- americanum group indicated that X. poasense n. sp. clustered with other species with a reduced anterior genital branch from the morphospecies Group 2, viz. , X. costaricense and X. krugi . However, the phylogeny of coxI and partial 18S rRNA gene revealed that the new species did not cluster with Xiphinema species having the anterior genital branch absent or reduced ( i.e. , morphospecies Groups 1 and 2, respectively).
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a new dagger nematode Xiphinema tica n sp nematoda longidoridae from costa rica with updating of the polytomous key of loof and luc 1990
European Journal of Plant Pathology, 2018Co-Authors: Walter Perazapadilla, Carolina Cantalapiedranavarrete, Pablo Castillo, Juan E Palomaresrius, Tatiana Zamoraaraya, Antonio ArchidonayusteAbstract:A new dagger nematode, Xiphinema tica n. sp., is described and illustrated from several populations extracted from soil associated with several crops and wild plants in Costa Rica. The new dagger nematode is characterised by a moderate body size (3276–4240 μm), a rounded lip region, ca 13.5 μm wide, separated from body contour by a shallow depression, amphidial fovea large, stirrup-shaped, a moderately long odontostyle ca 135 μm long, stylet guiding ring located at ca 122 μm from anterior end, vulva almost equatorial (50–54%), well-developed Z-organ, with heavy muscularised wall containing in the most of specimens observed two moderately refractive inclusions variable in shape (from round to star-shaped), with uterine spines and crystalloid bodies; female tail short, dorsally convex-conoid, with rounded end and a small peg, with a c’ ratio ca 0.8, bearing two or three pairs of caudal pores and male absent. The unique and novel uterine differentiation based on the coexistence of a well-developed Z-organ mixed with uterine spines and crystalloid bodies in Xiphinema prompted us to update and include this combination of characters in the polytomous key of Loof and Luc (1990). Integrative diagnosis was completed with molecular data obtained, using D2-D3 expansion segments of 28S rDNA, ITS1-rDNA, partial 18S–rDNA and the partial mitochondrial gene cytochrome c oxidase subunit 1 (coxI). The phylogenetic relationships of this species with other Xiphinema spp. indicated that X. tica n. sp. was monophyletic to the other species from the morphospecies Group 4, Xiphinema oleae.
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molecular characterization of pseudomonodelphic dagger nematodes of the genus Xiphinema cobb 1913 nematoda longidoridae in costa rica with notes on Xiphinema setariae tarjan 1964
European Journal of Plant Pathology, 2017Co-Authors: Walter Perazapadilla, Carolina Cantalapiedranavarrete, Antonio Archidonayuste, Juan E Palomaresrius, H Ferris, Tatiana Zamoraaraya, Sergei A Subbotin, Pablo CastilloAbstract:Pseudomonodelphic dagger nematodes of the genus Xiphinema are characterized by having one of the genital branches reduced and lacking an ovary. They are usually reported from tropical regions. Nematode surveys conducted during rainy seasons in Costa Rica resulted in detection of several Xiphinema costaricense populations, but also other pseudomonodelphic and didelphic species of Xiphinema. We undertook detailed integrative morphometric and molecular studies using D2-D3 expansion segments of 28S rDNA, and ITS1-rDNA. From those studies, we also identified several populations of Xiphinema krugi and two populations of Xiphinema setariae with characteristics in agreement with those of the original and later descriptions of these species. The phylogenetic analyses of these species with other representatives of Xiphinema spp. indicated that pseudomonodelphic species are phylogenetically related (X. costaricense and X. krugi). On the basis of ITS1 sequences of X. costaricense and X. variegatum from Brazil, as well as similar morphology and morphometrics of both species, the latter is proposed here as a junior synonym of the former. Also, our morphometric data showed some intraspecific variability within X. setariae, which in combination with the molecular evidence, suggests that X. setariae and X. vulgare need to be considered as a single taxon.
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molecular phylogenetic analysis and comparative morphology resolve two new species of olive tree soil related dagger nematodes of the genus Xiphinema dorylaimida longidoridae from spain
Invertebrate Systematics, 2016Co-Authors: Antonio Archidonayuste, Carolina Cantalapiedranavarrete, Juan A Navascortes, Juan E Palomaresrius, Pablo CastilloAbstract:The genus Xiphinema constitutes a large group of ~275 species of polyphagous, plant-ectoparasitic nematodes that are distributed almost worldwide. Some species affect agricultural crops by feeding directly on root cells as well as by transmitting nepoviruses. Despite their agricultural importance, species discrimination in Xiphinema is difficult, leading to potential misidentification. Integrative taxonomy, based on the combination of molecular analyses and morphology, constitutes a new insight into Xiphinema species identification. In this study we describe two new species of Xiphinema from the Iberian Peninsula (X. macrodora, sp. nov. and X. oleae, sp. nov.) associated with cultivated and wild olive trees. Both species have specific rRNA sequences. Morphologically, Xiphinema macrodora, sp. nov. is characterised by a very long body (7.2–8.7 mm), a very long odontostyle and odontophore (190–206 and 105–120 µm, respectively), and a well developed pseudo-Z-organ, comprising 8 to 12 sclerotised bodies. Xiphinema oleae, sp. nov. is characterised by an odontostyle and an odontophore 136–149 and 65–80 µm long, respectively, and a well developed Z-organ with refractive inclusions (3–5), variable in shape. Additionally, X. macrodora, sp. nov. has the longest body size, and the longest odontostyle and odontophore of any Xiphinema, whereas X. oleae, sp. nov. is the first species with a well developed Z-organ from the Iberian Peninsula. http://zoobank.org/urn:lsid:zoobank.org:pub:E4CE8711-22EF-40E4-9D39-6FE962F25225
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remarkable diversity and prevalence of dagger nematodes of the genus Xiphinema cobb 1913 nematoda longidoridae in olives revealed by integrative approaches
PLOS ONE, 2016Co-Authors: Antonio Archidonayuste, Carolina Cantalapiedranavarrete, Juan A Navascortes, Juan E Palomaresrius, Pablo CastilloAbstract:: The genus Xiphinema includes a remarkable group of invertebrates of the phylum Nematoda comprising ectoparasitic animals of many wild and cultivated plants. Damage is caused by direct feeding on root cells and by vectoring nepoviruses that cause diseases on several crops. Precise identification of Xiphinema species is critical for launching appropriate control measures. We make available the first detailed information on the diversity and distribution of Xiphinema species infesting wild and cultivated olive in a wide-region in southern Spain that included 211 locations from which 453 sampling sites were analyzed. The present study identified thirty-two Xiphinema spp. in the rhizosphere of olive trees, ten species belonging to Xiphinema americanum-group, whereas twenty-two were attributed to Xiphinema non-americanum-group. These results increase our current knowledge on the biodiversity of Xiphinema species identified in olives and include the description of four new species (Xiphinema andalusiense sp. nov., Xiphinema celtiense sp. nov., Xiphinema iznajarense sp. nov., and Xiphinema mengibarense sp. nov.), and two new records for cultivate olives (X. cadavalense and X. conurum). We also found evidence of remarkable prevalence of Xiphinema spp. in olive trees, viz. 85.0% (385 out of 453 sampling sites), and they were widely distributed in both wild and cultivated olives, with 26 and 17 Xiphinema spp., respectively. Diversity indexes (Richness, Hill´s diversity, Hill´s reciprocal of D and Hill´s evenness) were significantly affected by olive type. We also developed a comparative morphological and morphometrical study together with molecular data from three nuclear ribosomal RNA genes (D2-D3 expansion segments of 28S, ITS1, and partial 18S). Molecular characterization and phylogenetic analyses allowed the delimitation and discrimination of four new species of the genus described herein and three known species. Phylogenetic analyses of Xiphinema spp. resulted in a general consensus of these species groups. This study is the most complete phylogenetic analysis for Xiphinema non-americanum-group species to date.
Juan E Palomaresrius - One of the best experts on this subject based on the ideXlab platform.
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a new dagger nematode Xiphinema poasense n sp nematoda longidoridae from costa rica
Nematology, 2018Co-Authors: Ingrid Varelabenavides, Carolina Cantalapiedranavarrete, Pablo Castillo, Juan E Palomaresrius, Walter Perazapadilla, Antonio ArchidonayusteAbstract:A new dagger nematode, Xiphinema poasense n. sp., is described and illustrated from three populations extracted from soil associated with a combined plantation of Eucalyptus sp., Cupressus sp. and Pennisetum sp. and wild plants from a tropical pre-montane forest in Costa Rica. The new dagger nematode is characterised by a moderate body size 2612 (2416-3042) μ m long, a rounded lip region 15.0 (13.5-16.5) μ m broad, separated from the body contour by a shallow depression, amphidial fovea large, stirrup-shaped, a very long odontostyle (175 (164-188) μ m), stylet guiding ring located 167 (136-181) μ m from anterior end, vulva situated anterior to mid-body (36-40%), anterior genital branch complete but strongly reduced, without uterine differentiation, female tail short, hemispherical to convex-conoid with a c′ ratio = 0.7 (0.6-0.8) and bearing two pairs of caudal pores, and male absent. Integrative diagnosis was completed with molecular data using D2-D3 expansion segments of 28S rRNA, ITS1 region, partial 18S-rRNA and the partial mitochondrial gene cytochrome c oxidase subunit 1 ( coxI ). The phylogenetic relationships based on D2-D3 segments of this species with other Xiphinema spp. of the X. non- americanum group indicated that X. poasense n. sp. clustered with other species with a reduced anterior genital branch from the morphospecies Group 2, viz. , X. costaricense and X. krugi . However, the phylogeny of coxI and partial 18S rRNA gene revealed that the new species did not cluster with Xiphinema species having the anterior genital branch absent or reduced ( i.e. , morphospecies Groups 1 and 2, respectively).
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a new dagger nematode Xiphinema tica n sp nematoda longidoridae from costa rica with updating of the polytomous key of loof and luc 1990
European Journal of Plant Pathology, 2018Co-Authors: Walter Perazapadilla, Carolina Cantalapiedranavarrete, Pablo Castillo, Juan E Palomaresrius, Tatiana Zamoraaraya, Antonio ArchidonayusteAbstract:A new dagger nematode, Xiphinema tica n. sp., is described and illustrated from several populations extracted from soil associated with several crops and wild plants in Costa Rica. The new dagger nematode is characterised by a moderate body size (3276–4240 μm), a rounded lip region, ca 13.5 μm wide, separated from body contour by a shallow depression, amphidial fovea large, stirrup-shaped, a moderately long odontostyle ca 135 μm long, stylet guiding ring located at ca 122 μm from anterior end, vulva almost equatorial (50–54%), well-developed Z-organ, with heavy muscularised wall containing in the most of specimens observed two moderately refractive inclusions variable in shape (from round to star-shaped), with uterine spines and crystalloid bodies; female tail short, dorsally convex-conoid, with rounded end and a small peg, with a c’ ratio ca 0.8, bearing two or three pairs of caudal pores and male absent. The unique and novel uterine differentiation based on the coexistence of a well-developed Z-organ mixed with uterine spines and crystalloid bodies in Xiphinema prompted us to update and include this combination of characters in the polytomous key of Loof and Luc (1990). Integrative diagnosis was completed with molecular data obtained, using D2-D3 expansion segments of 28S rDNA, ITS1-rDNA, partial 18S–rDNA and the partial mitochondrial gene cytochrome c oxidase subunit 1 (coxI). The phylogenetic relationships of this species with other Xiphinema spp. indicated that X. tica n. sp. was monophyletic to the other species from the morphospecies Group 4, Xiphinema oleae.
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molecular characterization of pseudomonodelphic dagger nematodes of the genus Xiphinema cobb 1913 nematoda longidoridae in costa rica with notes on Xiphinema setariae tarjan 1964
European Journal of Plant Pathology, 2017Co-Authors: Walter Perazapadilla, Carolina Cantalapiedranavarrete, Antonio Archidonayuste, Juan E Palomaresrius, H Ferris, Tatiana Zamoraaraya, Sergei A Subbotin, Pablo CastilloAbstract:Pseudomonodelphic dagger nematodes of the genus Xiphinema are characterized by having one of the genital branches reduced and lacking an ovary. They are usually reported from tropical regions. Nematode surveys conducted during rainy seasons in Costa Rica resulted in detection of several Xiphinema costaricense populations, but also other pseudomonodelphic and didelphic species of Xiphinema. We undertook detailed integrative morphometric and molecular studies using D2-D3 expansion segments of 28S rDNA, and ITS1-rDNA. From those studies, we also identified several populations of Xiphinema krugi and two populations of Xiphinema setariae with characteristics in agreement with those of the original and later descriptions of these species. The phylogenetic analyses of these species with other representatives of Xiphinema spp. indicated that pseudomonodelphic species are phylogenetically related (X. costaricense and X. krugi). On the basis of ITS1 sequences of X. costaricense and X. variegatum from Brazil, as well as similar morphology and morphometrics of both species, the latter is proposed here as a junior synonym of the former. Also, our morphometric data showed some intraspecific variability within X. setariae, which in combination with the molecular evidence, suggests that X. setariae and X. vulgare need to be considered as a single taxon.
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molecular phylogenetic analysis and comparative morphology resolve two new species of olive tree soil related dagger nematodes of the genus Xiphinema dorylaimida longidoridae from spain
Invertebrate Systematics, 2016Co-Authors: Antonio Archidonayuste, Carolina Cantalapiedranavarrete, Juan A Navascortes, Juan E Palomaresrius, Pablo CastilloAbstract:The genus Xiphinema constitutes a large group of ~275 species of polyphagous, plant-ectoparasitic nematodes that are distributed almost worldwide. Some species affect agricultural crops by feeding directly on root cells as well as by transmitting nepoviruses. Despite their agricultural importance, species discrimination in Xiphinema is difficult, leading to potential misidentification. Integrative taxonomy, based on the combination of molecular analyses and morphology, constitutes a new insight into Xiphinema species identification. In this study we describe two new species of Xiphinema from the Iberian Peninsula (X. macrodora, sp. nov. and X. oleae, sp. nov.) associated with cultivated and wild olive trees. Both species have specific rRNA sequences. Morphologically, Xiphinema macrodora, sp. nov. is characterised by a very long body (7.2–8.7 mm), a very long odontostyle and odontophore (190–206 and 105–120 µm, respectively), and a well developed pseudo-Z-organ, comprising 8 to 12 sclerotised bodies. Xiphinema oleae, sp. nov. is characterised by an odontostyle and an odontophore 136–149 and 65–80 µm long, respectively, and a well developed Z-organ with refractive inclusions (3–5), variable in shape. Additionally, X. macrodora, sp. nov. has the longest body size, and the longest odontostyle and odontophore of any Xiphinema, whereas X. oleae, sp. nov. is the first species with a well developed Z-organ from the Iberian Peninsula. http://zoobank.org/urn:lsid:zoobank.org:pub:E4CE8711-22EF-40E4-9D39-6FE962F25225
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remarkable diversity and prevalence of dagger nematodes of the genus Xiphinema cobb 1913 nematoda longidoridae in olives revealed by integrative approaches
PLOS ONE, 2016Co-Authors: Antonio Archidonayuste, Carolina Cantalapiedranavarrete, Juan A Navascortes, Juan E Palomaresrius, Pablo CastilloAbstract:: The genus Xiphinema includes a remarkable group of invertebrates of the phylum Nematoda comprising ectoparasitic animals of many wild and cultivated plants. Damage is caused by direct feeding on root cells and by vectoring nepoviruses that cause diseases on several crops. Precise identification of Xiphinema species is critical for launching appropriate control measures. We make available the first detailed information on the diversity and distribution of Xiphinema species infesting wild and cultivated olive in a wide-region in southern Spain that included 211 locations from which 453 sampling sites were analyzed. The present study identified thirty-two Xiphinema spp. in the rhizosphere of olive trees, ten species belonging to Xiphinema americanum-group, whereas twenty-two were attributed to Xiphinema non-americanum-group. These results increase our current knowledge on the biodiversity of Xiphinema species identified in olives and include the description of four new species (Xiphinema andalusiense sp. nov., Xiphinema celtiense sp. nov., Xiphinema iznajarense sp. nov., and Xiphinema mengibarense sp. nov.), and two new records for cultivate olives (X. cadavalense and X. conurum). We also found evidence of remarkable prevalence of Xiphinema spp. in olive trees, viz. 85.0% (385 out of 453 sampling sites), and they were widely distributed in both wild and cultivated olives, with 26 and 17 Xiphinema spp., respectively. Diversity indexes (Richness, Hill´s diversity, Hill´s reciprocal of D and Hill´s evenness) were significantly affected by olive type. We also developed a comparative morphological and morphometrical study together with molecular data from three nuclear ribosomal RNA genes (D2-D3 expansion segments of 28S, ITS1, and partial 18S). Molecular characterization and phylogenetic analyses allowed the delimitation and discrimination of four new species of the genus described herein and three known species. Phylogenetic analyses of Xiphinema spp. resulted in a general consensus of these species groups. This study is the most complete phylogenetic analysis for Xiphinema non-americanum-group species to date.
Wilfrida Decraemer - One of the best experts on this subject based on the ideXlab platform.
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first report morphological and molecular characterization of Xiphinema elongatum and x pachtaicum nematoda longidoridae from ethiopia
ZooKeys, 2015Co-Authors: Gezahegne Getaneh, Wim Bert, Wilfrida DecraemerAbstract:A total of six soil samples were collected around rhizosphere of citrus plants during 2010 from Melkassa Agricultural Research Center experimental station, Ethiopia. From these samples two most important ecto-plant parasitic nematodes of the genus Xiphinema were found and analysed. The genus Xiphinema is a large group of the phylum nematoda which constitutes more than 260 species. They are polyphagous root- ectoparasites of many crop plants and some species of this genus cause damage by direct feeding on root tips and transmit nepoviruses. The delimitation and discrimination of two species in the genus is presented, described herein as Xiphinema elongatum and Xiphinema pachtaicum. Morphological and morphometric data were done using light microscopy and results of both species were fit within the previously described nematode species of X. elongatum and X. pachtaicum. 18S rDNA were analysed using Bayesian inference (BI) method to reconstruct phylogenetic relationships of the studied Xiphinema sp. (KP407872 X. elongatum and KP407873 X. pachtaicum) with other Xiphinema species. The 18S rDNA sequence of X. pachtaicum was alike to previously described species from the GenBank but X. elongatum exhibited very small levels of nucleotides differences (0.4%) which might be possible intra-specific divergence. Though this region of rDNA has less resolution on complex species, its combination with morphological and morphometric analyses, suggests these species as X. elongatum and X. pachtaicum with the GenBank ac
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phylogeny diversity and species delimitation in some species of the Xiphinema americanum group complex nematoda longidoridae as inferred from nuclear and mitochondrial dna sequences and morphology
European Journal of Plant Pathology, 2012Co-Authors: Carlos Gutierrezgutierrez, Wilfrida Decraemer, Carolina Cantalapiedranavarrete, Nicola Vovlas, Tom Prior, Juan Palomares E Rius, Pablo CastilloAbstract:During nematode surveys in southern Spain and Italy 14 populations of Xiphinema species tentatively identified as Xiphinema americanum-group were detected. Morphological and morphometrical studies identified three new species and six known Xiphinema americanum-group species, viz.: Xiphinema parabrevicolle n. sp., Xiphinema parapachydermum n. sp., Xiphinema paratenuicutis n. sp., Xiphinema duriense, Xiphinema incertum, Xiphinema opisthohysterum, Xiphinema pachtaicum, Xiphinema rivesi, and Xiphinema santos. The Xiphinema americanum-group is the most difficult Xiphinema species group for diagnosis since the morphology is very conservative and morphometric characters often overlap. This group includes vectors of several important plant pathogenic viruses that cause significant damage to a wide range of agricultural crops. Molecular characterisation of these species using D2-D3 expansion regions of 28S rRNA, 18S rRNA, ITS1-rRNA and the protein-coding mitochondrial gene, cytochrome oxidase c subunit 1 was carried out and maximum likelihood and Bayesian inference analysis were used to reconstruct phylogenetic relationships among these species and with other Xiphinema americanum-group species.
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molecular characterization of Xiphinema brevicollum nematoda longidoridae from the czech republic
European Journal of Plant Pathology, 2010Co-Authors: Shesh Kumari, Wilfrida Decraemer, Francesca De LucaAbstract:Populations of Xiphinema brevicollum occurring in the Czech Republic were described morphologically and molecularly. Published species-specific primer set BL18 and BV3 was used to amplify three populations of X. brevicollum from the Czech Republic. These primers were tested against 9 species of Xiphinema and 11 species of Longidorus. Amplification was also observed for X. inaequale, X. italiae and X. lambertii. Three additional markers, mitochondrial cytochrome c oxidase subunit 1, ribosomal D2/D3 expansion segment of the 28S gene and 18S gene, were amplified and sequenced for X. brevicollum, X. inaequale and X. lambertii belonging to X. americanum-group. Comparison of cox1 sequences of X. brevicollum from the Czech Republic with X. taylori from the Slovak Republic (accession number AM086702) suggested that these populations represent the same species.
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A single bivulval female of Xiphinema diversicaudatum and two bivulval females of Xiphinema vuittenezi (Nematoda: Longidoridae)
Helminthologia, 2009Co-Authors: Shesh Kumari, Wilfrida DecraemerAbstract:A population of Xiphinema vuittenezi from an apple orchard and a population of X. diversicaudatum from a peach orchard yielded bivulval females. Morphometrics of these females are presented.
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first report of the dagger nematode Xiphinema dentatum nematoda longidoridae in a deciduous forest in the czech republic
Plant Disease, 2008Co-Authors: S. Kumari, Wilfrida DecraemerAbstract:Xiphinema species are migratory ectoparasitic nematodes that feed on an extensive range of hosts and several species are vectors of nepoviruses. These long nematodes are readily distinguished from most other plant parasitic nematodes by a long stylet with forked odontostyle and flanged odontophore. In May of 2005, a sample from the rhizosphere of Carpinus betulus and Acer platanoides in a forest near Silnicna, South Moravia yielded a population of Xiphinema dentatum Sturhan, 1978. X. dentatum previously has been reported to be associated with several forest and grassland species in Germany, the former Yugoslavia, and Slovakia. Specimens were extracted from soil by decanting-sieving. A few female specimens were stored at –20°C in 1 M NaCl, and the rest of the specimens were heat killed, fixed in triethanolamine formalin, and mounted in anhydrous glycerin. In 2007, nematodes from permanent slides were identified by morphological and morphometrical characters (3): female body C shaped in fixed specimens, lip...
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distribution and morphometrical characterization of Xiphinema pachtaicum x simile and x brevicollum from hungary
Helminthologia, 2008Co-Authors: V Repasi, A. Agostinelli, P Nagy, M I Coiro, K Hecker, F LambertiAbstract:The distribution of species of the Xiphinema americanumgroup in Hungary was studied by collecting 272 samples from 53 localities. Samples have been taken from soil in the rhizosphere of 70 plant species. In total, 12.86 % of the samples contained at least one species from the Xiphinema americanum-group. Three species were found: Xiphinema brevicollum, X. pachtaicum and X. simile. Xiphinema brevicollum occurred in 4.41 %, X. pachtaicum in 3.67 % and X. simile in 4.77 % of the samples. New data on plants associated with the reported species and developmental patterns for X. brevicollum and X. pachtaicum are also presented.
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On the occurence of Xiphinema index Thorne et Allen in Chile
2004Co-Authors: J. C. Magunacelaya, F Lamberti, M. T. AhumadaAbstract:A nematode survey was carried out during the years 1998-2004 in Chile to assess the occurrence of the dagger nematode, Xiphinema index, in vineyards and on fig trees grown in backyards around dwellings. Approximately 45% of samples were positive for the nematode. The nematode occurred more frequently in the northern and central Regions than in the southern ones. Xiphinema index was not detected in Regions IX and X.
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the Xiphinema americanum group 3 keys to species identification
Nematologia Mediterranea (Italy), 2004Co-Authors: F Lamberti, A. Agostinelli, Maurice Moens, S Hockland, D. J. F. BrownAbstract:A polytomous and a dichotomous key for specific identification of 49 putative species of the Xiphinema americanum group are presented. Priority is given to quantitative characters to minimize dependency on subjective judgements. Among the characters selected odontostyle length, ratios c and c', and V% appeared the most reliable for examining intra- and inter-population relationships.
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the occurrence and geographic distribution of Xiphinema and xiphidorus species nematoda longidoridae in brazil
Helminthologia, 2003Co-Authors: C M G Oliveira, D. J. F. Brown, Roy Neilson, Ailton Rocha Monteiro, L C C B Ferraz, F LambertiAbstract:41 Summary The occurrence and geographic distribution of Xiphinema and Xiphidorus species were investigated during a national survey of 16 Brazilian States between 1999 and 2001. Eighty-two soil samples were collected from different habitats, including crop plants, grassland, savanna (cerra-do) and typical Amazonian forest vegetation. Fourteen Xi-phinema species ( X. brasiliense, X. brevicolle, X. elonga-tum, X. ensiculiferum, X. ifacolum, X. krugi, X. longicau-datum, X. paritaliae, X. setariae/vulgare complex , X. suri-namense, X. variegatum, and three morphotypes of X. ame-ricanum sensu lato identified as representing X. diffusum, X. oxycaudatum and X. peruvianum ) were recorded. Also, Xiphidorus balcarceanus , X. minor , X. yepesara parthenus and X. yepesara yepesara were identified from three States, however recognition of X. yepesara parthenus as a subspecies of X. yepesara requires confirmation. The most frequently occurring species were X. krugi (46 % of all samples), X. brasiliense (22 %) and X. setariae/vulgare complex (22 %). Xiphinema diffusum, X. longicaudatum, X. oxycaudatum, X. peruvianum and Xiphidorus balcar-ceanus constitute new records for Brazil. A principal com-ponent analysis identified odontostyle and odontophore length and to a lesser extent vulva position as the main influencing morphological characters that could be used to separate the Xiphinema and Xiphidorus species recorded in this study. A dichotomous key for the identification of Xiphinema species reported in Brazil is presented. Keywords: identification key; survey; taxonomy; virus vector nematodes; Xiphidorus; Xiphinema Introduction Xiphinema nematodes have a world-wide distribution and cause substantial damage to an extensive range of crop plants by their direct feeding on plant root cells (Taylor and Brown, 1997; Weischer and Brown, 2000) and affect-ing plant growth (Lamberti et al. 1987a; 1992a; 1992b; 1993; Leone et al., 1997; 1999). Also, several of these spe-cies are important economically as they are efficient vec-tors of several members of the genus Nepovirus that cause diseases in a wide range of fruit and vegetable crops (Brown et al. , 1995, 1996). In a comprehensive review, Doucet et al. (1998) reported that forty-two Xiphinema species occur in Latin America, with twenty-two (X. basiri, X. brasiliense, X. brevicolle, X. californicum, X. clavicaudatum, X. costaricense, X. dimi-diatum, X. elongatum, X. ensuculiferum, X. fluminense, X. georgianum, X. guillaumeti, X. ifacolum, X. index, X. kru-gi, X. machoni, X. pachtaicum, X. parasetariae, X. parita-liae, X. paulistanum, X. setariae/X. vulgare complex and X. surinamense ), including several virus-vector species, having been recorded in Brazil. Recently, another three species of Xiphinema ( X. torvum, X. variegatum and X. vi-carium) were reported from Brazil (Siddiqi, 2000). Xiphidorus nematodes that are indigenous to Latin Ameri-ca have a more restricted distribution as compared with Xiphinema, and the economic importance of these nemato-des with respect to crop damage is unknown (Leone et al., 1999). Currently, only eight Xiphidorus species have been identified from the following South American countries: Argentina, Brazil, Uruguay and Venezuela (Decraemer et al. , 1996; Coomans et al. , 1996; Doucet et al. , 1998; Lam-berti et al. , 1999) and an undescribed species from Bolivia (Hunt, 1993). In Brazil, X. amazonensis, X. minor, X. yepe-sara parthenus, and X. yepesara yepesara have been pre-viously recorded (Doucet et al. , 1998). Here we present data from a national survey of 16 Brazi-H
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Xiphinema spp in vineyards of istra peninsula in croatia
Helminthologia, 2002Co-Authors: Marija Ivezic, Emilija Raspudic, F Lamberti, D J PribeticAbstract:In the present study sixty-one vineyards have been analyzed for presence of Xiphinema spp. on different localities of Istra Peninsula. Xiphinema spp. were found in 37, 70% of the samples. Of all examined vineyards, Xiphinema index Thorne & Allen, 1950 was present in 8, 20%, Xiphinema pachtaicum (Tulaganov, 1938) Kirjanova, 1951 in 16, 39% and Xiphinema spp. in 13, 11%.
