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Robert J Miller - One of the best experts on this subject based on the ideXlab platform.
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Preliminary assessment of Acaricide Resistance in cattle tick Rhipicephalus (Boophilus) microplus populations from the Caribbean island of Martinique. [p081]
2016Co-Authors: Stéphanie Depraz, Robert J Miller, Leonore Lovis, Manon Hamon, Philippe Pelonde, Lionel Felixine, Marie-claire Timir, Christophe Dalibard, Adalberto A. Pérez De León, Linroy ChristianAbstract:Context: Ticks and Tick-borne diseases (T&TBD) remain major constraints to livestock development in the Caribbean. The continuous use of Acaricides to control ticks is costly for livestock producers. Chemicals were used extensively in the Caribbean during Tropical Bont Tick programs between 1995 and 2005. The Cattle Fever Tick (CFT), Rhipicephalus (Boophilus) microplus, is known to develop multiResistance to Acaricides. However little is known about Acaricide Resistance epidemiology in the Caribbean, where CFT is endemic. Recently, several countries reported to the T&TBD working group of the Caribbean Animal Health Network (CaribVET) increasing cases of lack of acaracide efficacy on ruminants, especially in Martinique. Purpose: A 2-year project “ResisT” involving researchers, tick experts, veterinary services and farmer associations has been established to address Resistance in CFT Caribbean populations. Results of a pilot study conducted in Martinique are reported here. Methods: The Larval Tarsal Test, an innovative test developed by Novartis, was implemented at CIRAD laboratory in Guadeloupe. An exploratory survey was conducted in early 2015 to collect engorged female ticks from cattle, and to study tick control practices of 50 volunteer farmers. Five Acaricides from 3 major classes commonly used in the French West Indies were tested: synthetic pyrethroids, amidines and organophosphates. A susceptible strain maintained at the USDA-ARS CFTRL in Texas was used as reference to calculate Resistance ratios. Results: Preliminary results will be reported during this presentation. Conclusions: Study results and other epidemiological data on Acaricide Resistance and information on control practices will be integrated to develop tools and awareness materials for Caribbean farmers. Relevance: Studies like the one reported are needed to formulate strategies to prevent Acaricide Resistance development by adopting integrated tick control strategies. Knowledge gaps identified during the study enabled CaribVET to prioritize relevant avenues of research and to build collaborations in support of the veterinary services in the region to improve T&TBD surveillance and control. (Texte integral)
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Acaricide Resistance of rhipicephalus boophilus microplus in state of mato grosso do sul brazil
Revista Brasileira De Parasitologia Veterinaria, 2011Co-Authors: Renato Andreotti, Robert J Miller, Felix D Guerrero, Mariana Aparecida Soares, Jacqueline Cavalacante Barros, Adalberto Perez A De LeonAbstract:This study was conducted to obtain an epidemiological view of Acaricide Resistance in populations of Rhipicephalus (Boophilus) microplus in the State of Mato Grosso do Sul. Twenty-four tick samples were collected from municipalities in the State where farmers had reported concerns about Resistance to or failure of tick control. These ticks were subjected to in vitro Resistance detection assays using the adult immersion test (AIT). The efficacy of alpha-cypermethrin, cypermethrin and amitraz treatments on samples collected throughout the State was generally poor. AIT showed efficacy ≥ 90% from the use of DDVP + chlorfenvinphos) (20 out of 21 municipalities), dichlorvos + cypermethrin (10 out of 16 municipalities) and cypermethrin + citronella + chlorpyrifos + piperonyl butoxide (20 out of 21 municipalities). PCR assays were used to detect the presence of pyrethroid Resistance-associated sodium channel gene mutation. Larvae from three different populations that had previously been diagnosed as pyrethroid-resistant, through AIT, were evaluated. The PCR assays showed that the pyrethroid Resistance-associated gene mutation was absent from these three populations. This study confirms that the emergence of Resistance is a constant challenge for the livestock industry, and that development of Resistance continues to be a major driver for new antiparasitic drugs to be developed.
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Acaricide Resistance of rhipicephalus boophilus microplus in state of mato grosso do sul brazil resistencia do rhipicephalus boophilus microplus aos acaricidas no estado de mato grosso do sul brasil
2011Co-Authors: Renato Andreotti, Robert J Miller, Felix D Guerrero, Mariana Aparecida Soares, Adalberto Perez A De Leon, Jacqueline Cavalcante Barros, Usdaars KniplingbushlandAbstract:This study was conducted to obtain an epidemiological view of Acaricide Resistance in populations of Rhipicephalus (Boophilus) microplus in the State of Mato Grosso do Sul. Twenty-four tick samples were collected from municipalities in the State where farmers had reported concerns about Resistance to or failure of tick control. These ticks were subjected to in vitro Resistance detection assays using the adult immersion test (AIT). The efficacy of alpha-cypermethrin, cypermethrin and amitraz treatments on samples collected throughout the State was generally poor. AIT showed efficacy ≥ 90% from the use of DDVP + chlorfenvinphos) (20 out of 21 municipalities), dichlorvos + cypermethrin (10 out of 16 municipalities) and cypermethrin + citronella + chlorpyrifos + piperonyl butoxide (20 out of 21 municipalities). PCR assays were used to detect the presence of pyrethroid Resistance-associated sodium channel gene mutation. Larvae from three different populations that had previously been diagnosed as pyrethroid-resistant, through AIT, were evaluated. The PCR assays showed that the pyrethroid Resistance-associated gene mutation was absent from these three populations. This study confirms that the emergence of Resistance is a constant challenge for the livestock industry, and that development of Resistance continues to be a major driver for new antiparasitic drugs to be developed.
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Genetic basis and impact of tick Acaricide Resistance.
Frontiers in bioscience (Landmark edition), 2009Co-Authors: R. Rosario-cruz, Ruben Hernandez-ortiz, Robert J Miller, Consuelo Almazán, Delia Ines Dominguez-garcia, José De La FuenteAbstract:Acaricide Resistance in Boophilus microplus has been studied for the last 20 years from the toxicology, metabolic and genomic points of view, however, only few methods for molecular detection of Resistance have been developed. Despite the relatively poor sensitivity for Resistance detection, bioassays remain the method of choice for susceptibility evaluation of tick populations, based on their toxicological response after exposure to Acaricides. Metabolic detoxification of Acaricides is known to be mediated by multigene- families of enzymes such as GST, Esterases and Mixed Function Oxidases (cytochrome P450). In addition, target site insensitivity has been studied on the sodium channel and acetylcholinesterase genes. The use of genomics to understand Acaricide Resistance in B. microplus will play a major role in unraveling the molecular mechanisms of Resistance. Advances in genomics, will accelerate the development of new diagnostic and immunoprophylactic tools based on new vaccine candidates, and new molecular targets for Acaricide Resistance detection and improvement of strategies for the control of ticks and tick-borne diseases in tropical and subtropical areas of Mexico.
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Inheritance of pyrethroid Resistance and a sodium channel gene mutation in the cattle tick Boophilus microplus
Parasitology Research, 2008Co-Authors: G. Aguilar-tipacamú, Roger Iván Rodríguez-vivas, Z. García-vázquez, Ruben Hernandez-ortiz, C. Vásquez-peláez, F. Olvera-valencia, Robert J Miller, R. Rosario-cruzAbstract:A substitution (Phe→Ile) within the sodium channel gene sequence has been associated with pyrethroid Resistance in Boophilus microplus . The aim of the present study was to analyze the inheritance of pyrethroid Resistance and the mutant allele, on reciprocal crosses of a susceptible (SS) and a resistant (RR) strain. Bioassays and genotypes were determined to evaluate pyrethroid Resistance. The Resistance allele frequency of both parental strains were 100% and 2.27% for RR and SS, respectively. The reciprocal crosses show a predominance of the heterozygote genotype, in agreement with the significant decrease of the Acaricide Resistance to cypermethrin, deltamethrin, and flumethrin. However, the RS progeny showed a complete recessive survival ( D _ML = 0) for deltamethrin and flumethrin, suggesting a complete dominance of the susceptible allele and incomplete dominance for cypermethrin ( D _ML = 0.169). On the other hand, SR progeny showed a partially recessive survival for cypermethrin ( D _ML = 0.380), deltamethrin ( D _ML = 0.319), and flumethrin ( D _ML = 0.258), indicative of a partially dominant inheritance of the Resistance. A possible maternal strain effect should be considered for practical purposes and prediction of Acaricide Resistance and further work needs to be done to elucidate the underlying inheritance of pyrethroid Resistance and the sodium channel mutation in B. microplus .
Fang Zhu - One of the best experts on this subject based on the ideXlab platform.
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Mechanisms and management of Acaricide Resistance for Tetranychus urticae in agroecosystems
Journal of Pest Science, 2021Co-Authors: Adekunle W. Adesanya, Mark D. Lavine, Fang Zhu, Timothy W. Moural, Laura C. Lavine, Douglas B WalshAbstract:The two-spotted spider mite, Tetranychus urticae Koch, is a constant threat to sustainable production of numerous economically important crops globally. Management of T. urticae is heavily reliant on the application of synthetic Acaricides. However, T. urticae has rapidly developed Resistance to most of the Acaricides available for its control due to its very broad host plant range, extremely short lifecycle, high fecundity, arrhenotokous parthenogenesis, and overwintering strategy. Despite the recent progress in identifying genetic changes/markers associated with Resistance toward some commonly used Acaricides for T. urticae , there is still limited studies that select and apply these markers in field populations to guide sustainable pest management strategy design. Temporal and spatial characterization of Acaricide-resistant phenotypes and their underlying mechanisms are crucial for the design and implementation of successful and sustainable integrated mite management programs. This review highlights the current Acaricide Resistance status of field-collected T. urticae populations and the underlying molecular mechanisms of Resistance. Our review found that some genetic mutations in target sites and/or overexpression of metabolic genes confer Resistance in geographically exclusive populations, while some Resistance markers appear to be specific to populations at biogeographical areas. Thus, there is a need for locally based coordinated efforts to understand the mechanisms of Resistance present in endemic T. urticae populations. Moreover, we discuss a prospective template for designing an effective Acaricide Resistance management program within various agroecosystems.
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rna interference of nadph cytochrome p450 reductase increases susceptibilities to multiple Acaricides in tetranychus urticae
Pesticide Biochemistry and Physiology, 2020Co-Authors: Adekunle W. Adesanya, Mark D. Lavine, Laura Corley Lavine, Antonio Cardenas, Douglas B Walsh, Fang ZhuAbstract:The two-spotted spider mite, Tetranychus urticae, is a polyphagous pest feeding on over 1100 plant species, including numerous highly valued economic crops. The control of T. urticae largely depends on the use of Acaricides, which leads to pervasive development of Acaricide Resistance. Cytochrome P450-mediated metabolic detoxification is one of the major mechanisms of Acaricide Resistance in T. urticae. NADPH-cytochrome P450 reductase (CPR) plays as a crucial co-factor protein that donates electron(s) to microsomal cytochrome P450s to complete their catalytic cycle. This study seeks to understand the involvement of CPR/P450 in Acaricide Resistance in T. urticae. The full-length cDNA sequence of T. urticae's CPR (TuCPR) was cloned and characterized. TuCPR was ubiquitously transcribed in different life stages of T. urticae and the highest transcription was observed in the nymph and adult stages. TuCPR was constitutively over-expressed in six Acaricide resistant populations compared to a susceptible one. TuCPR transcriptional expression was also induced by multiple Acaricides in a time-dependent manner. Down-regulation of TuCPR via RNA interference (RNAi) in T. urticae led to reduced enzymatic activities of TuCPR and cytochrome P450s, as well as a reduction of Resistance to multiple Acaricides, abamectin, bifenthrin, and fenpyroximate. The outcome of this study highlights CPR as a potential novel target for eco-friendly control of T. urticae and other related plant-feeding pests.
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Physiological Resistance alters behavioral response of Tetranychus urticae to Acaricides.
Scientific Reports, 2019Co-Authors: Adekunle W. Adesanya, Michael J. Beauchamp, Mark D. Lavine, Laura Corley Lavine, Fang Zhu, Doug B. WalshAbstract:Multiple Acaricide Resistance in Tetranychus urticae continues to threaten crop production globally, justifying the need to adequately study Resistance for sustainable pest management. Most studies on Acaricide Resistance have focused on the acute contact toxicity of Acaricides with little or no information on the behavioral responses elicited after Acaricide exposure. Furthermore, the impact of physiological Resistance on these behavioral responses remains unknown in most pest species, including T. urticae. We tested the effect of Acaricide Resistance on contact toxicity, irritancy and repellency of mitochondrial electron transport inhibitor of complex I (MET-I) and mite growth inhibitor (MGI) Acaricides on multiple T. urticae strains. We also tested whether Acaricides with similar physiological target site/mode of action also elicit similar behavioral effects on T. urticae strains. MET-I Acaricides (fenazaquin, fenpyroximate, and pyrabiden) and MGIs (clofentezine, hexythiazox and etoxazole) elicited a dose-dependent irritant and repellent effect on T. urticae. Selection of strains for physiological Resistance to these Acaricides affected the behavioral response of T. urticae, especially in MET-I resistant strains, that showed reduced irritancy and repellency to MET-I Acaricides. Behavioral response also affected the oviposition of T. urticae, where strains generally showed preferential oviposition away from the Acaricides. The outcome of this study highlights negative consequences of Acaricide Resistance that can potentially affect T. urticae management.
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rna interference of nadph cytochrome p450 reductase increases susceptibilities to multiple Acaricides in tetranychus urticae
bioRxiv, 2019Co-Authors: Adekunle W. Adesanya, Mark D. Lavine, Laura Corley Lavine, Antonio Cardenas, Douglas B Walsh, Fang ZhuAbstract:The two-spotted spider mite, Tetranychus urticae, is a polyphagous pest feeding on over 1,100 plant species, including numerous highly valued economic crops. The control of T. urticae largely depends on the use of Acaricides, which leads to pervasive development of Acaricide Resistance. Cytochrome P450-mediated metabolic detoxification is one of the major mechanisms of Acaricide Resistance in T. urticae. NADPH-cytochrome P450 reductase (CPR) plays as a crucial co-factor protein that donates electron(s) to microsomal cytochrome P450s to complete their catalytic cycle. This study seeks to understand the involvement of CPR in Acaricide Resistance in urticae. The full-length cDNA sequence of T. urticaes CPR (TuCPR) was cloned and characterized. TuCPR was ubiquitously transcribed in different life stages of T. urticae and the highest transcription was observed in the nymph and adult stages. TuCPR was constitutively over-expressed in six Acaricide resistant populations compared to a susceptible one. TuCPR transcriptional expression was also induced by multiple Acaricides in a time-dependent manner. Down-regulation of TuCPR via RNA interference (RNAi) in T. urticae led to reduced enzymatic activities of TuCPR and cytochrome P450s, as well as a significant reduction of Resistance to multiple Acaricides, abamectin, bifenthrin, and fenpyroximate. The outcome of this study highlights CPR as a potential novel target for eco-friendly control of T. urticae and other related plant-feeding pests.nnHighlightsO_LIPipernoyl butoxide significantly reduced abamectin, bifenthrin, and fenpyroximate Resistance in T. urticae populationsnC_LIO_LIT. urticaes cytochrome P450 reductase (TuCPR) was cloned, sequenced and phylogenetically analyzednC_LIO_LIAbamectin, bifenthrin and fenpyroximate treatment induced TuCPR gene expressionnC_LIO_LISilencing of TuCPR in T. urticae caused a reduction in Acaricide ResistancenC_LI
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phenotypic and genotypic plasticity of Acaricide Resistance in populations of tetranychus urticae acari tetranychidae on peppermint and silage corn in the pacific northwest
Journal of Economic Entomology, 2018Co-Authors: Adekunle W. Adesanya, Mark D. Lavine, Laura Corley Lavine, Douglas B Walsh, Elizabeth Franco, Fang ZhuAbstract:Tetranychus urticae Koch is a generalist pest of economic crops and is notorious for its rapid development of Acaricide Resistance. This poses a significant threat to the sustainability of integrated pest management (IPM) in cropping systems plagued by T. urticae. It is critical to evaluate the Resistance status of T. urticae populations on crops and identify any underlying Resistance mechanisms. This study investigated the efficacy of five major Acaricides on T. urticae populations on peppermint and silage corn in the Pacific Northwestern United States and identified the underlying Resistance mechanisms. Significant variations in Acaricide Resistance status of T. urticae populations were identified to abamectin, bifenthrin, fenpyroximate, hexythiazox, and spirodiclofen. In most cases, T. urticae populations from silage corn exhibited greater levels of Acaricide Resistance relative to peppermint populations. We detected known target-site mutations: F1534S and F1538I (conferring Resistance to bifenthrin), G126S (linked with Resistance to bifenazate), and I1017 (conferring Resistance to hexythiazox and etoxazole) in 10, 90, and 90% of the populations, respectively, from peppermint fields. These four mutations were identified in all the populations collected from silage corn fields. Significantly higher transcript levels of metabolic genes associated with Resistance to abamectin, fenpyroximate, and spirodiclofen were observed in some T. urticae populations collected from both peppermint and silage corn fields. This study provides evidence of multiple Resistance to diverse active ingredients in field populations of T. urticae and the reliability of known molecular markers for active Acaricide Resistance monitoring. The observed Resistance pattern will help in designing a sustainable IPM program for T. urticae.
Adekunle W. Adesanya - One of the best experts on this subject based on the ideXlab platform.
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Mechanisms and management of Acaricide Resistance for Tetranychus urticae in agroecosystems
Journal of Pest Science, 2021Co-Authors: Adekunle W. Adesanya, Mark D. Lavine, Fang Zhu, Timothy W. Moural, Laura C. Lavine, Douglas B WalshAbstract:The two-spotted spider mite, Tetranychus urticae Koch, is a constant threat to sustainable production of numerous economically important crops globally. Management of T. urticae is heavily reliant on the application of synthetic Acaricides. However, T. urticae has rapidly developed Resistance to most of the Acaricides available for its control due to its very broad host plant range, extremely short lifecycle, high fecundity, arrhenotokous parthenogenesis, and overwintering strategy. Despite the recent progress in identifying genetic changes/markers associated with Resistance toward some commonly used Acaricides for T. urticae , there is still limited studies that select and apply these markers in field populations to guide sustainable pest management strategy design. Temporal and spatial characterization of Acaricide-resistant phenotypes and their underlying mechanisms are crucial for the design and implementation of successful and sustainable integrated mite management programs. This review highlights the current Acaricide Resistance status of field-collected T. urticae populations and the underlying molecular mechanisms of Resistance. Our review found that some genetic mutations in target sites and/or overexpression of metabolic genes confer Resistance in geographically exclusive populations, while some Resistance markers appear to be specific to populations at biogeographical areas. Thus, there is a need for locally based coordinated efforts to understand the mechanisms of Resistance present in endemic T. urticae populations. Moreover, we discuss a prospective template for designing an effective Acaricide Resistance management program within various agroecosystems.
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rna interference of nadph cytochrome p450 reductase increases susceptibilities to multiple Acaricides in tetranychus urticae
Pesticide Biochemistry and Physiology, 2020Co-Authors: Adekunle W. Adesanya, Mark D. Lavine, Laura Corley Lavine, Antonio Cardenas, Douglas B Walsh, Fang ZhuAbstract:The two-spotted spider mite, Tetranychus urticae, is a polyphagous pest feeding on over 1100 plant species, including numerous highly valued economic crops. The control of T. urticae largely depends on the use of Acaricides, which leads to pervasive development of Acaricide Resistance. Cytochrome P450-mediated metabolic detoxification is one of the major mechanisms of Acaricide Resistance in T. urticae. NADPH-cytochrome P450 reductase (CPR) plays as a crucial co-factor protein that donates electron(s) to microsomal cytochrome P450s to complete their catalytic cycle. This study seeks to understand the involvement of CPR/P450 in Acaricide Resistance in T. urticae. The full-length cDNA sequence of T. urticae's CPR (TuCPR) was cloned and characterized. TuCPR was ubiquitously transcribed in different life stages of T. urticae and the highest transcription was observed in the nymph and adult stages. TuCPR was constitutively over-expressed in six Acaricide resistant populations compared to a susceptible one. TuCPR transcriptional expression was also induced by multiple Acaricides in a time-dependent manner. Down-regulation of TuCPR via RNA interference (RNAi) in T. urticae led to reduced enzymatic activities of TuCPR and cytochrome P450s, as well as a reduction of Resistance to multiple Acaricides, abamectin, bifenthrin, and fenpyroximate. The outcome of this study highlights CPR as a potential novel target for eco-friendly control of T. urticae and other related plant-feeding pests.
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Physiological Resistance alters behavioral response of Tetranychus urticae to Acaricides.
Scientific Reports, 2019Co-Authors: Adekunle W. Adesanya, Michael J. Beauchamp, Mark D. Lavine, Laura Corley Lavine, Fang Zhu, Doug B. WalshAbstract:Multiple Acaricide Resistance in Tetranychus urticae continues to threaten crop production globally, justifying the need to adequately study Resistance for sustainable pest management. Most studies on Acaricide Resistance have focused on the acute contact toxicity of Acaricides with little or no information on the behavioral responses elicited after Acaricide exposure. Furthermore, the impact of physiological Resistance on these behavioral responses remains unknown in most pest species, including T. urticae. We tested the effect of Acaricide Resistance on contact toxicity, irritancy and repellency of mitochondrial electron transport inhibitor of complex I (MET-I) and mite growth inhibitor (MGI) Acaricides on multiple T. urticae strains. We also tested whether Acaricides with similar physiological target site/mode of action also elicit similar behavioral effects on T. urticae strains. MET-I Acaricides (fenazaquin, fenpyroximate, and pyrabiden) and MGIs (clofentezine, hexythiazox and etoxazole) elicited a dose-dependent irritant and repellent effect on T. urticae. Selection of strains for physiological Resistance to these Acaricides affected the behavioral response of T. urticae, especially in MET-I resistant strains, that showed reduced irritancy and repellency to MET-I Acaricides. Behavioral response also affected the oviposition of T. urticae, where strains generally showed preferential oviposition away from the Acaricides. The outcome of this study highlights negative consequences of Acaricide Resistance that can potentially affect T. urticae management.
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rna interference of nadph cytochrome p450 reductase increases susceptibilities to multiple Acaricides in tetranychus urticae
bioRxiv, 2019Co-Authors: Adekunle W. Adesanya, Mark D. Lavine, Laura Corley Lavine, Antonio Cardenas, Douglas B Walsh, Fang ZhuAbstract:The two-spotted spider mite, Tetranychus urticae, is a polyphagous pest feeding on over 1,100 plant species, including numerous highly valued economic crops. The control of T. urticae largely depends on the use of Acaricides, which leads to pervasive development of Acaricide Resistance. Cytochrome P450-mediated metabolic detoxification is one of the major mechanisms of Acaricide Resistance in T. urticae. NADPH-cytochrome P450 reductase (CPR) plays as a crucial co-factor protein that donates electron(s) to microsomal cytochrome P450s to complete their catalytic cycle. This study seeks to understand the involvement of CPR in Acaricide Resistance in urticae. The full-length cDNA sequence of T. urticaes CPR (TuCPR) was cloned and characterized. TuCPR was ubiquitously transcribed in different life stages of T. urticae and the highest transcription was observed in the nymph and adult stages. TuCPR was constitutively over-expressed in six Acaricide resistant populations compared to a susceptible one. TuCPR transcriptional expression was also induced by multiple Acaricides in a time-dependent manner. Down-regulation of TuCPR via RNA interference (RNAi) in T. urticae led to reduced enzymatic activities of TuCPR and cytochrome P450s, as well as a significant reduction of Resistance to multiple Acaricides, abamectin, bifenthrin, and fenpyroximate. The outcome of this study highlights CPR as a potential novel target for eco-friendly control of T. urticae and other related plant-feeding pests.nnHighlightsO_LIPipernoyl butoxide significantly reduced abamectin, bifenthrin, and fenpyroximate Resistance in T. urticae populationsnC_LIO_LIT. urticaes cytochrome P450 reductase (TuCPR) was cloned, sequenced and phylogenetically analyzednC_LIO_LIAbamectin, bifenthrin and fenpyroximate treatment induced TuCPR gene expressionnC_LIO_LISilencing of TuCPR in T. urticae caused a reduction in Acaricide ResistancenC_LI
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phenotypic and genotypic plasticity of Acaricide Resistance in populations of tetranychus urticae acari tetranychidae on peppermint and silage corn in the pacific northwest
Journal of Economic Entomology, 2018Co-Authors: Adekunle W. Adesanya, Mark D. Lavine, Laura Corley Lavine, Douglas B Walsh, Elizabeth Franco, Fang ZhuAbstract:Tetranychus urticae Koch is a generalist pest of economic crops and is notorious for its rapid development of Acaricide Resistance. This poses a significant threat to the sustainability of integrated pest management (IPM) in cropping systems plagued by T. urticae. It is critical to evaluate the Resistance status of T. urticae populations on crops and identify any underlying Resistance mechanisms. This study investigated the efficacy of five major Acaricides on T. urticae populations on peppermint and silage corn in the Pacific Northwestern United States and identified the underlying Resistance mechanisms. Significant variations in Acaricide Resistance status of T. urticae populations were identified to abamectin, bifenthrin, fenpyroximate, hexythiazox, and spirodiclofen. In most cases, T. urticae populations from silage corn exhibited greater levels of Acaricide Resistance relative to peppermint populations. We detected known target-site mutations: F1534S and F1538I (conferring Resistance to bifenthrin), G126S (linked with Resistance to bifenazate), and I1017 (conferring Resistance to hexythiazox and etoxazole) in 10, 90, and 90% of the populations, respectively, from peppermint fields. These four mutations were identified in all the populations collected from silage corn fields. Significantly higher transcript levels of metabolic genes associated with Resistance to abamectin, fenpyroximate, and spirodiclofen were observed in some T. urticae populations collected from both peppermint and silage corn fields. This study provides evidence of multiple Resistance to diverse active ingredients in field populations of T. urticae and the reliability of known molecular markers for active Acaricide Resistance monitoring. The observed Resistance pattern will help in designing a sustainable IPM program for T. urticae.
Mark D. Lavine - One of the best experts on this subject based on the ideXlab platform.
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Mechanisms and management of Acaricide Resistance for Tetranychus urticae in agroecosystems
Journal of Pest Science, 2021Co-Authors: Adekunle W. Adesanya, Mark D. Lavine, Fang Zhu, Timothy W. Moural, Laura C. Lavine, Douglas B WalshAbstract:The two-spotted spider mite, Tetranychus urticae Koch, is a constant threat to sustainable production of numerous economically important crops globally. Management of T. urticae is heavily reliant on the application of synthetic Acaricides. However, T. urticae has rapidly developed Resistance to most of the Acaricides available for its control due to its very broad host plant range, extremely short lifecycle, high fecundity, arrhenotokous parthenogenesis, and overwintering strategy. Despite the recent progress in identifying genetic changes/markers associated with Resistance toward some commonly used Acaricides for T. urticae , there is still limited studies that select and apply these markers in field populations to guide sustainable pest management strategy design. Temporal and spatial characterization of Acaricide-resistant phenotypes and their underlying mechanisms are crucial for the design and implementation of successful and sustainable integrated mite management programs. This review highlights the current Acaricide Resistance status of field-collected T. urticae populations and the underlying molecular mechanisms of Resistance. Our review found that some genetic mutations in target sites and/or overexpression of metabolic genes confer Resistance in geographically exclusive populations, while some Resistance markers appear to be specific to populations at biogeographical areas. Thus, there is a need for locally based coordinated efforts to understand the mechanisms of Resistance present in endemic T. urticae populations. Moreover, we discuss a prospective template for designing an effective Acaricide Resistance management program within various agroecosystems.
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rna interference of nadph cytochrome p450 reductase increases susceptibilities to multiple Acaricides in tetranychus urticae
Pesticide Biochemistry and Physiology, 2020Co-Authors: Adekunle W. Adesanya, Mark D. Lavine, Laura Corley Lavine, Antonio Cardenas, Douglas B Walsh, Fang ZhuAbstract:The two-spotted spider mite, Tetranychus urticae, is a polyphagous pest feeding on over 1100 plant species, including numerous highly valued economic crops. The control of T. urticae largely depends on the use of Acaricides, which leads to pervasive development of Acaricide Resistance. Cytochrome P450-mediated metabolic detoxification is one of the major mechanisms of Acaricide Resistance in T. urticae. NADPH-cytochrome P450 reductase (CPR) plays as a crucial co-factor protein that donates electron(s) to microsomal cytochrome P450s to complete their catalytic cycle. This study seeks to understand the involvement of CPR/P450 in Acaricide Resistance in T. urticae. The full-length cDNA sequence of T. urticae's CPR (TuCPR) was cloned and characterized. TuCPR was ubiquitously transcribed in different life stages of T. urticae and the highest transcription was observed in the nymph and adult stages. TuCPR was constitutively over-expressed in six Acaricide resistant populations compared to a susceptible one. TuCPR transcriptional expression was also induced by multiple Acaricides in a time-dependent manner. Down-regulation of TuCPR via RNA interference (RNAi) in T. urticae led to reduced enzymatic activities of TuCPR and cytochrome P450s, as well as a reduction of Resistance to multiple Acaricides, abamectin, bifenthrin, and fenpyroximate. The outcome of this study highlights CPR as a potential novel target for eco-friendly control of T. urticae and other related plant-feeding pests.
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Physiological Resistance alters behavioral response of Tetranychus urticae to Acaricides.
Scientific Reports, 2019Co-Authors: Adekunle W. Adesanya, Michael J. Beauchamp, Mark D. Lavine, Laura Corley Lavine, Fang Zhu, Doug B. WalshAbstract:Multiple Acaricide Resistance in Tetranychus urticae continues to threaten crop production globally, justifying the need to adequately study Resistance for sustainable pest management. Most studies on Acaricide Resistance have focused on the acute contact toxicity of Acaricides with little or no information on the behavioral responses elicited after Acaricide exposure. Furthermore, the impact of physiological Resistance on these behavioral responses remains unknown in most pest species, including T. urticae. We tested the effect of Acaricide Resistance on contact toxicity, irritancy and repellency of mitochondrial electron transport inhibitor of complex I (MET-I) and mite growth inhibitor (MGI) Acaricides on multiple T. urticae strains. We also tested whether Acaricides with similar physiological target site/mode of action also elicit similar behavioral effects on T. urticae strains. MET-I Acaricides (fenazaquin, fenpyroximate, and pyrabiden) and MGIs (clofentezine, hexythiazox and etoxazole) elicited a dose-dependent irritant and repellent effect on T. urticae. Selection of strains for physiological Resistance to these Acaricides affected the behavioral response of T. urticae, especially in MET-I resistant strains, that showed reduced irritancy and repellency to MET-I Acaricides. Behavioral response also affected the oviposition of T. urticae, where strains generally showed preferential oviposition away from the Acaricides. The outcome of this study highlights negative consequences of Acaricide Resistance that can potentially affect T. urticae management.
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rna interference of nadph cytochrome p450 reductase increases susceptibilities to multiple Acaricides in tetranychus urticae
bioRxiv, 2019Co-Authors: Adekunle W. Adesanya, Mark D. Lavine, Laura Corley Lavine, Antonio Cardenas, Douglas B Walsh, Fang ZhuAbstract:The two-spotted spider mite, Tetranychus urticae, is a polyphagous pest feeding on over 1,100 plant species, including numerous highly valued economic crops. The control of T. urticae largely depends on the use of Acaricides, which leads to pervasive development of Acaricide Resistance. Cytochrome P450-mediated metabolic detoxification is one of the major mechanisms of Acaricide Resistance in T. urticae. NADPH-cytochrome P450 reductase (CPR) plays as a crucial co-factor protein that donates electron(s) to microsomal cytochrome P450s to complete their catalytic cycle. This study seeks to understand the involvement of CPR in Acaricide Resistance in urticae. The full-length cDNA sequence of T. urticaes CPR (TuCPR) was cloned and characterized. TuCPR was ubiquitously transcribed in different life stages of T. urticae and the highest transcription was observed in the nymph and adult stages. TuCPR was constitutively over-expressed in six Acaricide resistant populations compared to a susceptible one. TuCPR transcriptional expression was also induced by multiple Acaricides in a time-dependent manner. Down-regulation of TuCPR via RNA interference (RNAi) in T. urticae led to reduced enzymatic activities of TuCPR and cytochrome P450s, as well as a significant reduction of Resistance to multiple Acaricides, abamectin, bifenthrin, and fenpyroximate. The outcome of this study highlights CPR as a potential novel target for eco-friendly control of T. urticae and other related plant-feeding pests.nnHighlightsO_LIPipernoyl butoxide significantly reduced abamectin, bifenthrin, and fenpyroximate Resistance in T. urticae populationsnC_LIO_LIT. urticaes cytochrome P450 reductase (TuCPR) was cloned, sequenced and phylogenetically analyzednC_LIO_LIAbamectin, bifenthrin and fenpyroximate treatment induced TuCPR gene expressionnC_LIO_LISilencing of TuCPR in T. urticae caused a reduction in Acaricide ResistancenC_LI
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phenotypic and genotypic plasticity of Acaricide Resistance in populations of tetranychus urticae acari tetranychidae on peppermint and silage corn in the pacific northwest
Journal of Economic Entomology, 2018Co-Authors: Adekunle W. Adesanya, Mark D. Lavine, Laura Corley Lavine, Douglas B Walsh, Elizabeth Franco, Fang ZhuAbstract:Tetranychus urticae Koch is a generalist pest of economic crops and is notorious for its rapid development of Acaricide Resistance. This poses a significant threat to the sustainability of integrated pest management (IPM) in cropping systems plagued by T. urticae. It is critical to evaluate the Resistance status of T. urticae populations on crops and identify any underlying Resistance mechanisms. This study investigated the efficacy of five major Acaricides on T. urticae populations on peppermint and silage corn in the Pacific Northwestern United States and identified the underlying Resistance mechanisms. Significant variations in Acaricide Resistance status of T. urticae populations were identified to abamectin, bifenthrin, fenpyroximate, hexythiazox, and spirodiclofen. In most cases, T. urticae populations from silage corn exhibited greater levels of Acaricide Resistance relative to peppermint populations. We detected known target-site mutations: F1534S and F1538I (conferring Resistance to bifenthrin), G126S (linked with Resistance to bifenazate), and I1017 (conferring Resistance to hexythiazox and etoxazole) in 10, 90, and 90% of the populations, respectively, from peppermint fields. These four mutations were identified in all the populations collected from silage corn fields. Significantly higher transcript levels of metabolic genes associated with Resistance to abamectin, fenpyroximate, and spirodiclofen were observed in some T. urticae populations collected from both peppermint and silage corn fields. This study provides evidence of multiple Resistance to diverse active ingredients in field populations of T. urticae and the reliability of known molecular markers for active Acaricide Resistance monitoring. The observed Resistance pattern will help in designing a sustainable IPM program for T. urticae.
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Mechanisms and management of Acaricide Resistance for Tetranychus urticae in agroecosystems
Journal of Pest Science, 2021Co-Authors: Adekunle W. Adesanya, Mark D. Lavine, Fang Zhu, Timothy W. Moural, Laura C. Lavine, Douglas B WalshAbstract:The two-spotted spider mite, Tetranychus urticae Koch, is a constant threat to sustainable production of numerous economically important crops globally. Management of T. urticae is heavily reliant on the application of synthetic Acaricides. However, T. urticae has rapidly developed Resistance to most of the Acaricides available for its control due to its very broad host plant range, extremely short lifecycle, high fecundity, arrhenotokous parthenogenesis, and overwintering strategy. Despite the recent progress in identifying genetic changes/markers associated with Resistance toward some commonly used Acaricides for T. urticae , there is still limited studies that select and apply these markers in field populations to guide sustainable pest management strategy design. Temporal and spatial characterization of Acaricide-resistant phenotypes and their underlying mechanisms are crucial for the design and implementation of successful and sustainable integrated mite management programs. This review highlights the current Acaricide Resistance status of field-collected T. urticae populations and the underlying molecular mechanisms of Resistance. Our review found that some genetic mutations in target sites and/or overexpression of metabolic genes confer Resistance in geographically exclusive populations, while some Resistance markers appear to be specific to populations at biogeographical areas. Thus, there is a need for locally based coordinated efforts to understand the mechanisms of Resistance present in endemic T. urticae populations. Moreover, we discuss a prospective template for designing an effective Acaricide Resistance management program within various agroecosystems.
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rna interference of nadph cytochrome p450 reductase increases susceptibilities to multiple Acaricides in tetranychus urticae
Pesticide Biochemistry and Physiology, 2020Co-Authors: Adekunle W. Adesanya, Mark D. Lavine, Laura Corley Lavine, Antonio Cardenas, Douglas B Walsh, Fang ZhuAbstract:The two-spotted spider mite, Tetranychus urticae, is a polyphagous pest feeding on over 1100 plant species, including numerous highly valued economic crops. The control of T. urticae largely depends on the use of Acaricides, which leads to pervasive development of Acaricide Resistance. Cytochrome P450-mediated metabolic detoxification is one of the major mechanisms of Acaricide Resistance in T. urticae. NADPH-cytochrome P450 reductase (CPR) plays as a crucial co-factor protein that donates electron(s) to microsomal cytochrome P450s to complete their catalytic cycle. This study seeks to understand the involvement of CPR/P450 in Acaricide Resistance in T. urticae. The full-length cDNA sequence of T. urticae's CPR (TuCPR) was cloned and characterized. TuCPR was ubiquitously transcribed in different life stages of T. urticae and the highest transcription was observed in the nymph and adult stages. TuCPR was constitutively over-expressed in six Acaricide resistant populations compared to a susceptible one. TuCPR transcriptional expression was also induced by multiple Acaricides in a time-dependent manner. Down-regulation of TuCPR via RNA interference (RNAi) in T. urticae led to reduced enzymatic activities of TuCPR and cytochrome P450s, as well as a reduction of Resistance to multiple Acaricides, abamectin, bifenthrin, and fenpyroximate. The outcome of this study highlights CPR as a potential novel target for eco-friendly control of T. urticae and other related plant-feeding pests.
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rna interference of nadph cytochrome p450 reductase increases susceptibilities to multiple Acaricides in tetranychus urticae
bioRxiv, 2019Co-Authors: Adekunle W. Adesanya, Mark D. Lavine, Laura Corley Lavine, Antonio Cardenas, Douglas B Walsh, Fang ZhuAbstract:The two-spotted spider mite, Tetranychus urticae, is a polyphagous pest feeding on over 1,100 plant species, including numerous highly valued economic crops. The control of T. urticae largely depends on the use of Acaricides, which leads to pervasive development of Acaricide Resistance. Cytochrome P450-mediated metabolic detoxification is one of the major mechanisms of Acaricide Resistance in T. urticae. NADPH-cytochrome P450 reductase (CPR) plays as a crucial co-factor protein that donates electron(s) to microsomal cytochrome P450s to complete their catalytic cycle. This study seeks to understand the involvement of CPR in Acaricide Resistance in urticae. The full-length cDNA sequence of T. urticaes CPR (TuCPR) was cloned and characterized. TuCPR was ubiquitously transcribed in different life stages of T. urticae and the highest transcription was observed in the nymph and adult stages. TuCPR was constitutively over-expressed in six Acaricide resistant populations compared to a susceptible one. TuCPR transcriptional expression was also induced by multiple Acaricides in a time-dependent manner. Down-regulation of TuCPR via RNA interference (RNAi) in T. urticae led to reduced enzymatic activities of TuCPR and cytochrome P450s, as well as a significant reduction of Resistance to multiple Acaricides, abamectin, bifenthrin, and fenpyroximate. The outcome of this study highlights CPR as a potential novel target for eco-friendly control of T. urticae and other related plant-feeding pests.nnHighlightsO_LIPipernoyl butoxide significantly reduced abamectin, bifenthrin, and fenpyroximate Resistance in T. urticae populationsnC_LIO_LIT. urticaes cytochrome P450 reductase (TuCPR) was cloned, sequenced and phylogenetically analyzednC_LIO_LIAbamectin, bifenthrin and fenpyroximate treatment induced TuCPR gene expressionnC_LIO_LISilencing of TuCPR in T. urticae caused a reduction in Acaricide ResistancenC_LI
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phenotypic and genotypic plasticity of Acaricide Resistance in populations of tetranychus urticae acari tetranychidae on peppermint and silage corn in the pacific northwest
Journal of Economic Entomology, 2018Co-Authors: Adekunle W. Adesanya, Mark D. Lavine, Laura Corley Lavine, Douglas B Walsh, Elizabeth Franco, Fang ZhuAbstract:Tetranychus urticae Koch is a generalist pest of economic crops and is notorious for its rapid development of Acaricide Resistance. This poses a significant threat to the sustainability of integrated pest management (IPM) in cropping systems plagued by T. urticae. It is critical to evaluate the Resistance status of T. urticae populations on crops and identify any underlying Resistance mechanisms. This study investigated the efficacy of five major Acaricides on T. urticae populations on peppermint and silage corn in the Pacific Northwestern United States and identified the underlying Resistance mechanisms. Significant variations in Acaricide Resistance status of T. urticae populations were identified to abamectin, bifenthrin, fenpyroximate, hexythiazox, and spirodiclofen. In most cases, T. urticae populations from silage corn exhibited greater levels of Acaricide Resistance relative to peppermint populations. We detected known target-site mutations: F1534S and F1538I (conferring Resistance to bifenthrin), G126S (linked with Resistance to bifenazate), and I1017 (conferring Resistance to hexythiazox and etoxazole) in 10, 90, and 90% of the populations, respectively, from peppermint fields. These four mutations were identified in all the populations collected from silage corn fields. Significantly higher transcript levels of metabolic genes associated with Resistance to abamectin, fenpyroximate, and spirodiclofen were observed in some T. urticae populations collected from both peppermint and silage corn fields. This study provides evidence of multiple Resistance to diverse active ingredients in field populations of T. urticae and the reliability of known molecular markers for active Acaricide Resistance monitoring. The observed Resistance pattern will help in designing a sustainable IPM program for T. urticae.
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Multiple Acaricide Resistance and underlying mechanisms in Tetranychus urticae on hops
Journal of Pest Science, 2018Co-Authors: Adekunle W. Adesanya, Mark D. Lavine, Laura Corley Lavine, Douglas B Walsh, Mariany A. Morales, Fang ZhuAbstract:The polyphagous pest Tetranychus urticae feeds on over 1100 plant species including highly valued economic crops such as hops (Humulus lupulus). In the key hop production region of the Pacific Northwest of the USA, T. urticae is one of the major arthropod pests. Over the years, T. urticae control has been dominated by the application of various Acaricides. However, T. urticae quickly adapts to these Acaricides by developing Resistance. Here, we determined Resistance ratios of T. urticae populations in hops to three Acaricides: etoxazole, fenpyroximate, and spirodiclofen. The mechanisms underlying Resistance to these and three other Acaricides were investigated in 37 field-collected T. urticae populations using a comprehensive diagnostic approach. Our data showed that T. urticae populations exhibited complex adaptation patterns to Acaricides. Resistance to abamectin, fenpyroximate, and spirodiclofen by enhanced target metabolic detoxification gene(s) was identified in 100%, 50%, and 20% of populations tested, respectively. Resistance to bifenthrin, bifenazate, and etoxazole by target site insensitivity was pervasive among tested populations. Our study provides new information in understanding the complexity of T. urticae adaptation to multiple Acaricides, which will help in designing sustainable pest control strategies for T. urticae on hops and other economically valuable crops.
