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Albumin Conjugate

The Experts below are selected from a list of 273 Experts worldwide ranked by ideXlab platform

Hae-sim Park – 1st expert on this subject based on the ideXlab platform

  • Occupational asthma induced by the reactive dye synozol red-K 3BS
    Allergy Asthma and Immunology Research, 2011
    Co-Authors: Hyun Jung Jin, Joo Hee Kim, Young-min Ye, Hae-sim Park

    Abstract:

    Various reactive dyes can elicit occupational asthma in exposed textile industry workers. To date, there has been no report of occupational asthma caused by the red dye Synozol Red-K 3BS (Red-K). Here, we report a 38-year-old male textile worker with occupational asthma and rhinitis induced by inhalation of Red-K. He showed positive responses to Red-K extract on skin-prick testing and serum specific IgE antibodies to Red-K-human serum Albumin Conjugate were detected using an enzyme-linked immunosorbent assay. A bronchoprovocation test with Red-K extract resulted in significant bronchoconstriction. These findings suggest that the inhalation of the reactive dye Red-K can induce IgE-mediated occupational asthma and rhinitis in exposed workers.

  • longitudinal study of specific antibodies to toluene diisocyanate tdi human serum Albumin hsa Conjugate in patients with tdi induced asthma
    The Korean Journal of Internal Medicine, 2002
    Co-Authors: Hae-sim Park, Dongho Nahm

    Abstract:

    Background :
    An appreciable number of patients with toluene diisocyanate (TDI)-induced asthma have high serum levels of specific IgE (sIgE) antibody to TDI-human serum Albumin Conjugate (HSA). A recent investigation suggested a role of specific IgG (sIgG) in the development of TDI asthma.

  • diverse profiles of specific ige response to toluene diisocyanate tdi human serum Albumin Conjugate in tdi induced asthma patients
    Journal of Korean Medical Science, 2001
    Co-Authors: Hae-sim Park, Dongho Nahm

    Abstract:

    The prevalence studies on specific IgE to toluene diisocyanate (TDI)-human serum Albumin (HSA) Conjugate in TDI-induced asthma have shown variable results. In this study, we attempted to compare specific IgE bindings to TDI-HSA Conjugate and its specificity using 3 different Conjugates. Sera were collected from 20 TDI-induced asthma and 10 controls. Specific IgE were measured by ELISA using three TDI-HSA Conjugates; two from Carnegie Mellon (CM; 98 and 99 CM Conjugates) and one from Ajou University. To evaluate specificity and cross-reactivity, ELISA inhibition tests were applied. Positive and negative predictive values between Ajou Conjugate and 98 CM Conjugate were 75% and 100%. Those between Ajou and 99 CM were 100% and 93.8%. One patient showed an isolated positive response to the Ajou with negative responses to the other two Conjugates. ELISA inhibition test using this patient’s serum revealed the significant inhibitions by the Ajou and minimal inhibitions by the others. On the other hand, another patient showed an isolated positive response to 99 CM with negative responses to the others, and ELISA inhibition test showed significant inhibition by 99 CM with minimal inhibitions by the others. These results suggest that specific IgE bindings to a new antigenic determinant of TDI-HSA Conjugate can be heterogeneous and differ from one individual to another.

Na Li – 2nd expert on this subject based on the ideXlab platform

  • a new enzyme immobilization technique based on thionine bovine serum Albumin Conjugate and gold colloidal nanoparticles for reagentless amperometric biosensor applications
    Electroanalysis, 2008
    Co-Authors: Shihong Chen, Ruo Yuan, Yaqin Chai, Na Li

    Abstract:

    A novel enzyme immobilization technique based on thionine-bovine serum Albumin Conjugate (Th-BSA) and gold colloidal nanoparticles (nano-Au) was developed. Thionine was covalently bound onto the BSA film with glutaraldehyde(GA) as cross-linker to achieve Th-BSA Conjugate. The free amino groups of thionine were then used to attach nano-Au for the immobilization of horseradish peroxidase (HRP). Such nano-Au/Th-BSA matrix shows a favorable microenvironment for retaining the native activity of the immobilized HRP and thionine immobilized in this way can effectively shuttle electrons between the electrode and the enzyme. The proposed biosensor displays excellent catalytic activity and rapid response for H2O2. The linear range for the determination of H2O2 is from 4.9×10−7 to 1.6×10−3 M with a detection limit of 2.1×10−7 M at 3σ and a Michaelies-Menten constant K value of 0.023 mM.

  • A New Enzyme Immobilization Technique Based on Thionine‐Bovine Serum Albumin Conjugate and Gold Colloidal Nanoparticles for Reagentless Amperometric Biosensor Applications
    Electroanalysis, 2008
    Co-Authors: Shihong Chen, Ruo Yuan, Yaqin Chai, Na Li

    Abstract:

    A novel enzyme immobilization technique based on thionine-bovine serum Albumin Conjugate (Th-BSA) and gold colloidal nanoparticles (nano-Au) was developed. Thionine was covalently bound onto the BSA film with glutaraldehyde(GA) as cross-linker to achieve Th-BSA Conjugate. The free amino groups of thionine were then used to attach nano-Au for the immobilization of horseradish peroxidase (HRP). Such nano-Au/Th-BSA matrix shows a favorable microenvironment for retaining the native activity of the immobilized HRP and thionine immobilized in this way can effectively shuttle electrons between the electrode and the enzyme. The proposed biosensor displays excellent catalytic activity and rapid response for H2O2. The linear range for the determination of H2O2 is from 4.9×10−7 to 1.6×10−3 M with a detection limit of 2.1×10−7 M at 3σ and a Michaelies-Menten constant K value of 0.023 mM.

Dongho Nahm – 3rd expert on this subject based on the ideXlab platform

  • longitudinal study of specific antibodies to toluene diisocyanate tdi human serum Albumin hsa Conjugate in patients with tdi induced asthma
    The Korean Journal of Internal Medicine, 2002
    Co-Authors: Hae-sim Park, Dongho Nahm

    Abstract:

    Background :
    An appreciable number of patients with toluene diisocyanate (TDI)-induced asthma have high serum levels of specific IgE (sIgE) antibody to TDI-human serum Albumin Conjugate (HSA). A recent investigation suggested a role of specific IgG (sIgG) in the development of TDI asthma.

  • diverse profiles of specific ige response to toluene diisocyanate tdi human serum Albumin Conjugate in tdi induced asthma patients
    Journal of Korean Medical Science, 2001
    Co-Authors: Hae-sim Park, Dongho Nahm

    Abstract:

    The prevalence studies on specific IgE to toluene diisocyanate (TDI)-human serum Albumin (HSA) Conjugate in TDI-induced asthma have shown variable results. In this study, we attempted to compare specific IgE bindings to TDI-HSA Conjugate and its specificity using 3 different Conjugates. Sera were collected from 20 TDI-induced asthma and 10 controls. Specific IgE were measured by ELISA using three TDI-HSA Conjugates; two from Carnegie Mellon (CM; 98 and 99 CM Conjugates) and one from Ajou University. To evaluate specificity and cross-reactivity, ELISA inhibition tests were applied. Positive and negative predictive values between Ajou Conjugate and 98 CM Conjugate were 75% and 100%. Those between Ajou and 99 CM were 100% and 93.8%. One patient showed an isolated positive response to the Ajou with negative responses to the other two Conjugates. ELISA inhibition test using this patient’s serum revealed the significant inhibitions by the Ajou and minimal inhibitions by the others. On the other hand, another patient showed an isolated positive response to 99 CM with negative responses to the others, and ELISA inhibition test showed significant inhibition by 99 CM with minimal inhibitions by the others. These results suggest that specific IgE bindings to a new antigenic determinant of TDI-HSA Conjugate can be heterogeneous and differ from one individual to another.