The Experts below are selected from a list of 273 Experts worldwide ranked by ideXlab platform
Hae-sim Park - One of the best experts on this subject based on the ideXlab platform.
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Occupational asthma induced by the reactive dye synozol red-K 3BS
Allergy Asthma and Immunology Research, 2011Co-Authors: Hyun Jung Jin, Joo Hee Kim, Young-min Ye, Hae-sim ParkAbstract:Various reactive dyes can elicit occupational asthma in exposed textile industry workers. To date, there has been no report of occupational asthma caused by the red dye Synozol Red-K 3BS (Red-K). Here, we report a 38-year-old male textile worker with occupational asthma and rhinitis induced by inhalation of Red-K. He showed positive responses to Red-K extract on skin-prick testing and serum specific IgE antibodies to Red-K-human serum Albumin Conjugate were detected using an enzyme-linked immunosorbent assay. A bronchoprovocation test with Red-K extract resulted in significant bronchoconstriction. These findings suggest that the inhalation of the reactive dye Red-K can induce IgE-mediated occupational asthma and rhinitis in exposed workers.
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longitudinal study of specific antibodies to toluene diisocyanate tdi human serum Albumin hsa Conjugate in patients with tdi induced asthma
The Korean Journal of Internal Medicine, 2002Co-Authors: Hae-sim Park, Dongho NahmAbstract:Background : An appreciable number of patients with toluene diisocyanate (TDI)-induced asthma have high serum levels of specific IgE (sIgE) antibody to TDI-human serum Albumin Conjugate (HSA). A recent investigation suggested a role of specific IgG (sIgG) in the development of TDI asthma.
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diverse profiles of specific ige response to toluene diisocyanate tdi human serum Albumin Conjugate in tdi induced asthma patients
Journal of Korean Medical Science, 2001Co-Authors: Hae-sim Park, Dongho NahmAbstract:The prevalence studies on specific IgE to toluene diisocyanate (TDI)-human serum Albumin (HSA) Conjugate in TDI-induced asthma have shown variable results. In this study, we attempted to compare specific IgE bindings to TDI-HSA Conjugate and its specificity using 3 different Conjugates. Sera were collected from 20 TDI-induced asthma and 10 controls. Specific IgE were measured by ELISA using three TDI-HSA Conjugates; two from Carnegie Mellon (CM; 98 and 99 CM Conjugates) and one from Ajou University. To evaluate specificity and cross-reactivity, ELISA inhibition tests were applied. Positive and negative predictive values between Ajou Conjugate and 98 CM Conjugate were 75% and 100%. Those between Ajou and 99 CM were 100% and 93.8%. One patient showed an isolated positive response to the Ajou with negative responses to the other two Conjugates. ELISA inhibition test using this patient's serum revealed the significant inhibitions by the Ajou and minimal inhibitions by the others. On the other hand, another patient showed an isolated positive response to 99 CM with negative responses to the others, and ELISA inhibition test showed significant inhibition by 99 CM with minimal inhibitions by the others. These results suggest that specific IgE bindings to a new antigenic determinant of TDI-HSA Conjugate can be heterogeneous and differ from one individual to another.
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Clinical and immunologic evaluations of reactive dye-exposed workers
The Journal of Allergy and Clinical Immunology, 1991Co-Authors: Hae-sim Park, Jae Hoon Roh, Yong Han Moon, Kyung Jong Lee, Byung Ok Kim, Mi-kyung Lee, Chein-soo HongAbstract:To evaluate type I hypersensitivity to reactive dyes, its prevalence, and its relationship to respiratory dysfunction, we studied clinical and immunologic features, including skin prick tests, RAST, and bronchoprovocation tests, of 309 employees working in a reactive-dye industry. Our survey revealed that 78 (25.2%) employees had work-related lower respiratory symptoms associated with or without nasal, skin, or eye symptoms. Among these employees, 38 (48.7%) had nonspecific bronchial reactivity. Thirteen demonstrated immediate (6), dual (6), or late only (1) asthmatic responses after inhalation of four kinds of reactive-dye solutions. Twenty-five employees demonstrated immediate skin responses to black GR dye, and 21 reacted to orange 3R. Fifty-three employees (17%) had specific serum IgE antibody against black GR and orange 3R-human serum Albumin Conjugate. Specific IgE was detected more frequently in symptomatic employees (30%) and smokers (100%). No association was found between atopy and specific IgE binding. The RAST-inhibition tests of black GR revealed significant inhibitions by black GR-human serum Albumin Conjugate and minimal inhibitions by unConjugated black GR. Orange 3R RAST-inhibition tests revealed significant inhibitions by Conjugated forms of black GR and orange 3R and some inhibitions by two unConjugated dyes, suggesting an immunologic cross-reactivity between these dyes. These findings suggested that reactive dyes could induce immunologic responses, most likely IgE-mediated. © 1991.
Na Li - One of the best experts on this subject based on the ideXlab platform.
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a new enzyme immobilization technique based on thionine bovine serum Albumin Conjugate and gold colloidal nanoparticles for reagentless amperometric biosensor applications
Electroanalysis, 2008Co-Authors: Shihong Chen, Ruo Yuan, Yaqin Chai, Na LiAbstract:A novel enzyme immobilization technique based on thionine-bovine serum Albumin Conjugate (Th-BSA) and gold colloidal nanoparticles (nano-Au) was developed. Thionine was covalently bound onto the BSA film with glutaraldehyde(GA) as cross-linker to achieve Th-BSA Conjugate. The free amino groups of thionine were then used to attach nano-Au for the immobilization of horseradish peroxidase (HRP). Such nano-Au/Th-BSA matrix shows a favorable microenvironment for retaining the native activity of the immobilized HRP and thionine immobilized in this way can effectively shuttle electrons between the electrode and the enzyme. The proposed biosensor displays excellent catalytic activity and rapid response for H2O2. The linear range for the determination of H2O2 is from 4.9×10−7 to 1.6×10−3 M with a detection limit of 2.1×10−7 M at 3σ and a Michaelies-Menten constant K value of 0.023 mM.
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A New Enzyme Immobilization Technique Based on Thionine‐Bovine Serum Albumin Conjugate and Gold Colloidal Nanoparticles for Reagentless Amperometric Biosensor Applications
Electroanalysis, 2008Co-Authors: Shihong Chen, Ruo Yuan, Yaqin Chai, Na LiAbstract:A novel enzyme immobilization technique based on thionine-bovine serum Albumin Conjugate (Th-BSA) and gold colloidal nanoparticles (nano-Au) was developed. Thionine was covalently bound onto the BSA film with glutaraldehyde(GA) as cross-linker to achieve Th-BSA Conjugate. The free amino groups of thionine were then used to attach nano-Au for the immobilization of horseradish peroxidase (HRP). Such nano-Au/Th-BSA matrix shows a favorable microenvironment for retaining the native activity of the immobilized HRP and thionine immobilized in this way can effectively shuttle electrons between the electrode and the enzyme. The proposed biosensor displays excellent catalytic activity and rapid response for H2O2. The linear range for the determination of H2O2 is from 4.9×10−7 to 1.6×10−3 M with a detection limit of 2.1×10−7 M at 3σ and a Michaelies-Menten constant K value of 0.023 mM.
Dongho Nahm - One of the best experts on this subject based on the ideXlab platform.
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longitudinal study of specific antibodies to toluene diisocyanate tdi human serum Albumin hsa Conjugate in patients with tdi induced asthma
The Korean Journal of Internal Medicine, 2002Co-Authors: Hae-sim Park, Dongho NahmAbstract:Background : An appreciable number of patients with toluene diisocyanate (TDI)-induced asthma have high serum levels of specific IgE (sIgE) antibody to TDI-human serum Albumin Conjugate (HSA). A recent investigation suggested a role of specific IgG (sIgG) in the development of TDI asthma.
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diverse profiles of specific ige response to toluene diisocyanate tdi human serum Albumin Conjugate in tdi induced asthma patients
Journal of Korean Medical Science, 2001Co-Authors: Hae-sim Park, Dongho NahmAbstract:The prevalence studies on specific IgE to toluene diisocyanate (TDI)-human serum Albumin (HSA) Conjugate in TDI-induced asthma have shown variable results. In this study, we attempted to compare specific IgE bindings to TDI-HSA Conjugate and its specificity using 3 different Conjugates. Sera were collected from 20 TDI-induced asthma and 10 controls. Specific IgE were measured by ELISA using three TDI-HSA Conjugates; two from Carnegie Mellon (CM; 98 and 99 CM Conjugates) and one from Ajou University. To evaluate specificity and cross-reactivity, ELISA inhibition tests were applied. Positive and negative predictive values between Ajou Conjugate and 98 CM Conjugate were 75% and 100%. Those between Ajou and 99 CM were 100% and 93.8%. One patient showed an isolated positive response to the Ajou with negative responses to the other two Conjugates. ELISA inhibition test using this patient's serum revealed the significant inhibitions by the Ajou and minimal inhibitions by the others. On the other hand, another patient showed an isolated positive response to 99 CM with negative responses to the others, and ELISA inhibition test showed significant inhibition by 99 CM with minimal inhibitions by the others. These results suggest that specific IgE bindings to a new antigenic determinant of TDI-HSA Conjugate can be heterogeneous and differ from one individual to another.
Shihong Chen - One of the best experts on this subject based on the ideXlab platform.
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a new enzyme immobilization technique based on thionine bovine serum Albumin Conjugate and gold colloidal nanoparticles for reagentless amperometric biosensor applications
Electroanalysis, 2008Co-Authors: Shihong Chen, Ruo Yuan, Yaqin Chai, Na LiAbstract:A novel enzyme immobilization technique based on thionine-bovine serum Albumin Conjugate (Th-BSA) and gold colloidal nanoparticles (nano-Au) was developed. Thionine was covalently bound onto the BSA film with glutaraldehyde(GA) as cross-linker to achieve Th-BSA Conjugate. The free amino groups of thionine were then used to attach nano-Au for the immobilization of horseradish peroxidase (HRP). Such nano-Au/Th-BSA matrix shows a favorable microenvironment for retaining the native activity of the immobilized HRP and thionine immobilized in this way can effectively shuttle electrons between the electrode and the enzyme. The proposed biosensor displays excellent catalytic activity and rapid response for H2O2. The linear range for the determination of H2O2 is from 4.9×10−7 to 1.6×10−3 M with a detection limit of 2.1×10−7 M at 3σ and a Michaelies-Menten constant K value of 0.023 mM.
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A New Enzyme Immobilization Technique Based on Thionine‐Bovine Serum Albumin Conjugate and Gold Colloidal Nanoparticles for Reagentless Amperometric Biosensor Applications
Electroanalysis, 2008Co-Authors: Shihong Chen, Ruo Yuan, Yaqin Chai, Na LiAbstract:A novel enzyme immobilization technique based on thionine-bovine serum Albumin Conjugate (Th-BSA) and gold colloidal nanoparticles (nano-Au) was developed. Thionine was covalently bound onto the BSA film with glutaraldehyde(GA) as cross-linker to achieve Th-BSA Conjugate. The free amino groups of thionine were then used to attach nano-Au for the immobilization of horseradish peroxidase (HRP). Such nano-Au/Th-BSA matrix shows a favorable microenvironment for retaining the native activity of the immobilized HRP and thionine immobilized in this way can effectively shuttle electrons between the electrode and the enzyme. The proposed biosensor displays excellent catalytic activity and rapid response for H2O2. The linear range for the determination of H2O2 is from 4.9×10−7 to 1.6×10−3 M with a detection limit of 2.1×10−7 M at 3σ and a Michaelies-Menten constant K value of 0.023 mM.
Felix Kratz - One of the best experts on this subject based on the ideXlab platform.
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the antitumor activity of a lactosaminated Albumin Conjugate of doxorubicin in a chemically induced hepatocellular carcinoma rat model compared to sorafenib
Digestive and Liver Disease, 2017Co-Authors: Bakheet E M Elsadek, André Warnecke, Ahmed M Mansour, Tahia H Saleem, Felix KratzAbstract:Abstract Background Worldwide, consistent survival benefit for chemotherapy in hepatocellular carcinoma (HCC) is a golden goal for concerned researchers. Nexavar® (sorafenib) is the only approved agent that achieved touchable successes in this regard. Thus, there is a pressing medical need for new promising drugs to improve HCC therapy. Aims our designed lactosaminated Albumin Conjugate of doxorubicin (L-HSA–DOXO) that rapidly and preferentially accumulates in the liver is compared, for the first time at its MTD, with doxorubicin and sorafenib, not only for antitumor efficacy but also for overall survival. Methods HCC was induced in male Wistar rats with N-nitrosodiethylamine added to drinking water (100 mg/L) for 8 weeks. Endpoints were antitumor efficacy, tolerability and overall survival. Results L-HSA–DOXO proved to be superior at least over doxorubicin in the majority of assessed endpoints. Circulating AFP-L3% was diminished in L-HSA–DOXO (14.5%) and sorafenib (18.4%) groups compared to DENA (31.1%) and doxorubicin (29.5%) groups. This superiority was further confirmed by Western blot analyses of some novel HCC biomarkers. Survival study reinforced consistent benefits of both L-HSA–DOXO and sorafenib. Conclusions L-HSA–DOXO shows at least comparable activity to sorafenib which clinically achieves only ∼3 months overall survival benefit. Combination of these two agents could act beneficially or synergistically via two different modes of action to fight HCC.
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Anticancer carrier-linked prodrugs in clinical trials
Expert Opinion on Investigational Drugs, 2007Co-Authors: Felix Kratz, Khalid Abu Ajaj, André WarneckeAbstract:Coupling of low molecular weight anticancer drugs to antibodies, serum proteins or polymers through a cleavable linker has been an effective method for improving the therapeutic index of cytotoxic established agents. Modern drug-antibody Conjugates that have recently entered clinical trials have primarily used highly potent drugs such as calicheamicin or maytansins. Gemtuzumab ozogamicin, a Conjugate of calicheamicin and an anti-CD33 humanized antibody, is the first drug-antibody Conjugate to receive market approval. Drug Conjugates that have undergone clinical assessment include N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer Conjugates with doxorubicin, camptothecin, paclitaxel and Pt(II) complexes, poly(ethylene glycol) Conjugates with camptothecin and paclitaxel, polyglutamate Conjugates with paclitaxel and camptothecin, a methotrexate-Albumin Conjugate and an Albumin-binding doxorubicin prodrug. This review summarizes the Phase I-III studies that have been performed with these macromolecular prodrugs.
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in vivo and in vitro efficacy of an acid sensitive Albumin Conjugate of adriamycin compared to the parent compound in murine renal cell carcinoma
Drug Delivery, 1999Co-Authors: Joachim Drevs, Inga Hofmann, Dieter Marme, Clemens Unger, Felix KratzAbstract:This investigation is part of a continuing effort to develop effective drug delivery systems for anticancer agents using human serum proteins as drug carriers. The purpose of the present study was to compare the in vitro and in vivo activity of Adriamycin and an acid-sensitive Adriamycin-Albumin Conjugate (A-HYD2) in murine renal-cell carcinoma (RENCA). The inhibitory efficacy of A-HYD in RENCA cells in vitro was approximately fourfold lower as compared to Adriamycin. In a first in vivo experiment the efficacy of AHYD2 was compared to free Adriamycin at equitoxic doses (doses: 2 x 12 mg/kg for Adriamycin; 4 x 12 mg/kg for A-HYD2, iv over a period of 2 weeks; beginning therapy at day 7 after tumor inoculation: 10 Balb/ c mice per group). Seven of the eight surviving mice in the Adriamycin-treated group manifested clearly visible kidney tumors and ascites at the end of the experiment. In contrast, the 10 mice treated with A-HYD2 showed a complete remission in all cases, with no visible primary tumor. In a s...
