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Aliivibrio fischeri

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Ágnes M. Móricz – 1st expert on this subject based on the ideXlab platform

  • Investigation of antibacterial and cytotoxic potential of phenolics derived from Cistus incanus L. by means of thin-layer chromatography-direct bioautography and cytotoxicity assay
    Journal of Liquid Chromatography & Related Technologies, 2018
    Co-Authors: Dariusz Szeremeta, Magdalena Knaś, Ewa Długosz, Klaudia Krzykała, Anna Mrozek-wilczkiewicz, Robert Musiol, Teresa Kowalska, Mieczysław Sajewicz, Ágnes M. Móricz

    Abstract:

    ABSTRACTCistus incanus L. (hairy rockrose) is a medicinal plant which belongs to the Cistaceae family and the Cistus genus, with a well established position in traditional medicine of the Mediterranean basin and the Middle East. It was the aim of this study to compare antibacterial activity of the phenolics derived from fourteen C. incanus samples of different origin (Turkey, Albania, Greece, and an unknown geographical location) obtained as herbal teas from a local market of diet supplements. This activity was assessed with the use of thin-layer chromatography–direct bioautography (TLC-DB) applied to crude extracts against the Gram negative naturally luminescent marine bacterium Aliivibrio fischeri and the Gram positive soil bacterium Bacillus subtilis as the test microorganisms. It was established that in spite of different origin of the investigated herbal samples, in qualitative terms their antibacterial activity was closely comparable and more strongly pronounced against the Gram positive than the Gr…

  • Effect-directed analysis via hyphenated high-performance thin-layer chromatography for bioanalytical profiling of sunflower leaves
    Journal of Chromatography A, 2017
    Co-Authors: Ágnes M. Móricz, Imanuel Yüce, András Darcsi, Szabolcs Béni, Gertrud E. Morlock

    Abstract:

    Abstract High-performance thin-layer chromatography (HPTLC) coupled with effect-directed analysis was used for non-targeted screening of sunflower leaf extract for components exhibiting antioxidant, antibacterial and/or cholinesterase enzyme inhibitory effects. The active compounds were characterized by HPTLC-electrospray ionization-high resolution mass spectrometry (ESI-HRMS) and HPTLC-Direct Analysis in Real Time (DART)-MS/MS. The latter ambient ionization technique (less soft than ESI) resulted in oxidation and fragmentation products and characteristic fragment ions. NMR spectroscopy after targeted isolation via preparative normal phase flash chromatography and semi-preparative reversed phase high-performance liquid chromatography supported the identification of two diterpenes to be (-)-kaur-16-en-19-oic acid and 15-α-angeloyloxy-ent-kaur-16-en-19-oic acid. Both compounds found to be multi-potent as they inhibited acetylcholinesterase and butyrylcholinesterase and showed antibacterial effects against Gram-positive Bacillus subtilis and Gram-negative Aliivibrio fischeri bacteria. Kaurenoic acid was also active against the Gram-negative pepper pathogenic Xanthomonas euvesicatoria bacteria.

  • Screening of antibacterial compounds in thymus vulgaris l. tincture using thin-layer chromatography-direct bioautography and liquid chromatography-tandem mass spectrometry techniques
    Jpc-journal of Planar Chromatography-modern Tlc, 2017
    Co-Authors: Wioleta Jesionek, Ágnes M. Móricz, Györgyi Horváth, Barbara Majer-dziedzic, Irena M Choma

    Abstract:

    In this study, thin-layer chromatography—direct bioautography (TLC—DB) was used for guiding the isolation and identification of antibacterial constituents of Thymus vulgaris L. ethanol extract. This TLC—bioassay method enables the separation and detection of active components directly on the surface of chromatographic plates. They can be identified by comparison with reference substances or using physicochemical methods, preferably spectroscopic ones (liquid chromatography—tandem mass spectrometry [LC—MS/MS], in the presented paper). The described method belongs to the effect-directed analyses (EDA). Seven bacterial strains were used as test organisms, both pathogenic and nonpathogenic, including methicillin-resistant Staphylococcus aureus as well as luminescent bacteria like Aliivibrio fischeri. Five fractions with the widest antimicrobial spectra were detected using TLC—DB, isolated by semi-preparative TLC and subjected to LC—MS/MS analyses. Finally, two bioactive components were tentatively identified,…

Irena M Choma – 2nd expert on this subject based on the ideXlab platform

  • Screening of Antibacterial Compounds in Thymus vulgaris L. Tincture Using Thin-Layer Chromatography—Direct Bioautography and Liquid Chromatography—Tandem Mass Spectrometry Techniques
    JPC – Journal of Planar Chromatography – Modern TLC, 2017
    Co-Authors: Wioleta Jesionek, Ágnes M. Móricz, Györgyi Horváth, Barbara Majer-dziedzic, Irena M Choma

    Abstract:

    In this study, thin-layer chromatography—direct bioautography (TLC—DB) was used for guiding the isolation and identification of antibacterial constituents of Thymus vulgaris L. ethanol extract. This TLC—bioassay method enables the separation and detection of active components directly on the surface of chromatographic plates. They can be identified by comparison with reference substances or using physicochemical methods, preferably spectroscopic ones (liquid chromatography—tandem mass spectrometry [LC—MS/MS], in the presented paper). The described method belongs to the effect-directed analyses (EDA). Seven bacterial strains were used as test organisms, both pathogenic and nonpathogenic, including methicillin-resistant Staphylococcus aureus as well as luminescent bacteria like Aliivibrio fischeri . Five fractions with the widest antimicrobial spectra were detected using TLC—DB, isolated by semi-preparative TLC and subjected to LC—MS/MS analyses. Finally, two bioactive components were tentatively identified, basing on their fragmentation pattern, as eriodictyol and 4,4′-dihydroxy-5,5′-diisopropyl-2,2′-dimethyl-3,6-bifenylodion.

  • Screening of antibacterial compounds in thymus vulgaris l. tincture using thin-layer chromatography-direct bioautography and liquid chromatography-tandem mass spectrometry techniques
    Jpc-journal of Planar Chromatography-modern Tlc, 2017
    Co-Authors: Wioleta Jesionek, Ágnes M. Móricz, Györgyi Horváth, Barbara Majer-dziedzic, Irena M Choma

    Abstract:

    In this study, thin-layer chromatography—direct bioautography (TLC—DB) was used for guiding the isolation and identification of antibacterial constituents of Thymus vulgaris L. ethanol extract. This TLC—bioassay method enables the separation and detection of active components directly on the surface of chromatographic plates. They can be identified by comparison with reference substances or using physicochemical methods, preferably spectroscopic ones (liquid chromatography—tandem mass spectrometry [LC—MS/MS], in the presented paper). The described method belongs to the effect-directed analyses (EDA). Seven bacterial strains were used as test organisms, both pathogenic and nonpathogenic, including methicillin-resistant Staphylococcus aureus as well as luminescent bacteria like Aliivibrio fischeri. Five fractions with the widest antimicrobial spectra were detected using TLC—DB, isolated by semi-preparative TLC and subjected to LC—MS/MS analyses. Finally, two bioactive components were tentatively identified,…

  • TLC-direct bioautography as a bioassay guided method for investigation of antibacterial compounds in Hypericum perforatum L.
    Journal of AOAC International, 2015
    Co-Authors: Wioleta Jesionek, Ágnes M. Móricz, Byula Kocsis, Györgyi Horváth, Ágnes Alberti, Irena M Choma

    Abstract:

    Fast high-throughput TLC-direct bioautography (DB) is an effect-directed analysis method that enables searching for biologically active (e.g., antimicrobial) substances in complex mixtures like plant extracts. The principle of the method is that separation and detection of biological properties of given mixture components is performed directly on a TLC plate. In searching for antibacterial activity, the developed plate is immersed in a bacterial broth, and bacteria grow directly on its layer during a proper incubation time. Inhibition zones are formed in places where antimicrobial components are located. The active compounds can be further identified using spectroscopic techniques. The aim of our study was investigation of plant components of Hypericum perforatum L. tincture by TLC-DB using nine bacterial strains: Micrococcus luteus, Bacillus subtilis, Escherichia coli, Staphylococcus aureus, methicillin-resistant S. aureus, S. epidermidis, Pseudomonas syringae pv. maculicola, Xanthomonas campestris pv. vesicatoria, and Aliivibrio fischeri. Compounds showing the widest range of antimicrobial activity were isolated using semipreparative TLC and identified as apigenin, 3,8′-biapigenin, quercetin, kaempferol, and linolenic acid by TLC, HPLC-diode array detection, and HPLC/MS/MS techniques.

Gertrud E. Morlock – 3rd expert on this subject based on the ideXlab platform

  • Effect-Directed Analysis of Pimpinella saxifraga L. Root Extract via HPTLC—UV/Vis/FLD—EDA—MS
    JPC – Journal of Planar Chromatography – Modern TLC, 2018
    Co-Authors: Gertrud E. Morlock, Tatjana Lapin

    Abstract:

    A method was developed for effect-directed analysis (EDA) of the root extract of Pimpinella saxifraga L. High-performance thin-layer chromatography (HPTLC) was hyphenated with microchemical, biochemical, and biological assays as well as electrospray ionization—mass spectrometry (ESI—MS). This HPTLC—UV/Vis/FLD—EDA—MS method directly pointed to multi-potent compounds in the P. saxifraga L. root extract. 2,2-Diphenyl-1-picrylhydrazyl radical scavengers, acetylcholinesterase inhibitors, estrogen-effective compounds, antimicrobials against Gram-positive Bacillus subtilis bacteria, and Gram-negative Aliivibrio fischeri bacteria were discovered in the root extract. A first targeted characterization of four unknown multi-potent compounds was performed by HPTLC—ESI—MS and microchemical derivatizations. This highly streamlined effect-directed profiling is recommended for a fast and cost-efficient natural product search.

  • Effect-directed analysis via hyphenated high-performance thin-layer chromatography for bioanalytical profiling of sunflower leaves
    Journal of Chromatography A, 2017
    Co-Authors: Ágnes M. Móricz, Imanuel Yüce, András Darcsi, Szabolcs Béni, Gertrud E. Morlock

    Abstract:

    Abstract High-performance thin-layer chromatography (HPTLC) coupled with effect-directed analysis was used for non-targeted screening of sunflower leaf extract for components exhibiting antioxidant, antibacterial and/or cholinesterase enzyme inhibitory effects. The active compounds were characterized by HPTLC-electrospray ionization-high resolution mass spectrometry (ESI-HRMS) and HPTLC-Direct Analysis in Real Time (DART)-MS/MS. The latter ambient ionization technique (less soft than ESI) resulted in oxidation and fragmentation products and characteristic fragment ions. NMR spectroscopy after targeted isolation via preparative normal phase flash chromatography and semi-preparative reversed phase high-performance liquid chromatography supported the identification of two diterpenes to be (-)-kaur-16-en-19-oic acid and 15-α-angeloyloxy-ent-kaur-16-en-19-oic acid. Both compounds found to be multi-potent as they inhibited acetylcholinesterase and butyrylcholinesterase and showed antibacterial effects against Gram-positive Bacillus subtilis and Gram-negative Aliivibrio fischeri bacteria. Kaurenoic acid was also active against the Gram-negative pepper pathogenic Xanthomonas euvesicatoria bacteria.

  • Planar chromatographic screening and quantification of coumarin in food, confirmed by mass spectrometry.
    Food Chemistry, 2017
    Co-Authors: Stephanie Krüger, Luisa Winheim, Gertrud E. Morlock

    Abstract:

    Abstract A sensitive quantitative screening of coumarin in 43 commercially available cinnamons and cinnamon-containing foods was developed via HPTLC. Complex samples like cinnamon, tea, breakfast cereals, milk rice, jam, cinnamon stars and buns were extracted with methanol only. Separation was performed on silica gel with a mixture of n- hexane, ethyl acetate and ammonia. The specific detection via derivatization with an ethanolic potassium hydroxide solution resulted in fluorescent coumarin zones, measured at 365/>400 nm after stabilization. Limits of detection and quantification were 200 and 400 pg/band, respectively. Over all different sample types, the contents ranged from 0.3 to 5129 mg/kg with a mean repeatability and mean intermediate precision of 4% each. HPTLC-MS of selected zones, eluted via the TLC-MS Interface into MS, confirmed the identity of coumarin. Effect-directed detection as bioanalytical tool for risk assessment showed coumarin to be active against Aliivibrio fischeri bacteria down to 100 ng/band.