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Artemia franciscana

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Leandro Sastre – One of the best experts on this subject based on the ideXlab platform.

  • Characterization of a functional serum response element in the Actin403 gene promoter from the crustacean Artemia franciscana
    FEBS Journal, 2001
    Co-Authors: Marie-carmen Casero, Leandro Sastre

    Abstract:

    The serum response factor (SRF) activates expression of several genes in response to growth factors present in serum. SRF also regulates the expression of tissue-specific genes, including those in vertebrate muscles. An SRF-binding site (CArG box) present in the Artemia franciscana Actin403 promoter was shown to be necessary for transcriptional activity in cultured cells from Drosophila melanogaster and mammals. This DNA region bound mammalian and Drosophila SRFs in vitro and mediated transcriptional activation of the Actin403 promoter in response to serum, phorbol esters and lysophosphatidic acid in transfected cultured mammalian cells. Mutations in the CArG box greatly reduced promoter activity and stimulation by extracellular compounds.

  • A serum response factor homologue is expressed in ectodermal tissues during development of the crustacean Artemia franciscana.
    Mechanisms of Development, 2000
    Co-Authors: Marie-carmen Casero, Leandro Sastre

    Abstract:

    Abstract Complementary DNA clones have been isolated from the crustacean Artemia franciscana coding for a serum response factor (SRF)-homologue that is more than 96% identical to human and Drosophila melanogaster SRFs in their MADS boxes. The SRF homologue is expressed in ectodermal tissues, as determined by in situ hybridization experiments. A SRF-binding site has been identified in the promoter region of the Actin403 gene that is also expressed in ectodermal tissues, in accordance with its transcriptional regulation by the SRF homologue. The mRNA coding for A. franciscana SRF is present at similar levels in cryptobiotic encysted embryos and in developing nauplii. However, there is a significant increase in CArG-binding activity at the later developmental stage, indicating a postranscriptional regulation of SRF during A. franciscana embryonic development.

  • Tissue-specific expression of two Artemia franciscana sarco/endoplasmic reticulum Ca-ATPase isoforms.
    Journal of Histochemistry and Cytochemistry, 1996
    Co-Authors: Ricardo Escalante, Leandro Sastre

    Abstract:

    The sarco/endoplasmic reticulum Ca-ATPase (SERCA) gene from Artemia franciscana is transcribed into two mRNAs that code for two different enzyme isoforms. We investigated the tissue-specific expression of each mRNA by in situ hybridization of larval tissue sections. One of the isoforms is expressed in the muscle fibers of the appendages. The other isoform is generally expressed throughout all tissues of the larvae. The tissue distribution of these two isoforms is very similar to the one described for the two homologous isoforms generated from the vertebrate SERCA 2 gene, and shows the evolutionarily conserved nature of their tissue-specific expression.

Erko Stackebrandt – One of the best experts on this subject based on the ideXlab platform.

  • exiguobacterium mexicanum sp nov and exiguobacterium Artemiae sp nov isolated from the brine shrimp Artemia franciscana
    Systematic and Applied Microbiology, 2006
    Co-Authors: Alejandro Lopezcortes, Peter Schumann, Rudiger Pukall, Erko Stackebrandt

    Abstract:

    Abstract Two Gram-positive strains isolated from cysts of the brine shrimp Artemia franciscana were subjected to a polyphasic taxonomic analysis. Based on 16S rRNA gene sequence comparison and composition of isoprenoid quinones, peptidoglycan and fatty acids, these organisms are members of the genus Exiguobacterium. Both strains showed 95.9% 16S rRNA gene sequence similarity to one another. The 16S rRNA gene sequences of strain 8NT and 9ANT were 97.5% and 98.9% similar to those of Exiguobacterium aurantiacum DSM 6208T and Exiguobacterium undae DSM 14481T, respectively. Based on differences in chemotaxonomic and physiological characteristics, results of DNA–DNA hybridization and automated riboprinting, two novel species of the genus Exiguobacterium are proposed, Exiguobacterium mexicanum sp. nov. (type strain 8NT=DSM 16483T=CIP 108859T) and Exiguobacterium Artemiae sp. nov. (type strain 9ANT=DSM 16484T=CIP 108858T).

  • exiguobacterium mexicanum sp nov and exiguobacterium Artemiae sp nov isolated from the brine shrimp Artemia franciscana
    Systematic and Applied Microbiology, 2006
    Co-Authors: Alejandro Lopezcortes, Peter Schumann, Rudiger Pukall, Erko Stackebrandt

    Abstract:

    Abstract Two Gram-positive strains isolated from cysts of the brine shrimp Artemia franciscana were subjected to a polyphasic taxonomic analysis. Based on 16S rRNA gene sequence comparison and composition of isoprenoid quinones, peptidoglycan and fatty acids, these organisms are members of the genus Exiguobacterium. Both strains showed 95.9% 16S rRNA gene sequence similarity to one another. The 16S rRNA gene sequences of strain 8NT and 9ANT were 97.5% and 98.9% similar to those of Exiguobacterium aurantiacum DSM 6208T and Exiguobacterium undae DSM 14481T, respectively. Based on differences in chemotaxonomic and physiological characteristics, results of DNA–DNA hybridization and automated riboprinting, two novel species of the genus Exiguobacterium are proposed, Exiguobacterium mexicanum sp. nov. (type strain 8NT=DSM 16483T=CIP 108859T) and Exiguobacterium Artemiae sp. nov. (type strain 9ANT=DSM 16484T=CIP 108858T).

Patrick Sorgeloos – One of the best experts on this subject based on the ideXlab platform.

  • Impact of brine acidification on hatchability, survival and reproduction of Artemia parthenogenetica and Artemia franciscana in salt ponds, Bohai Bay, China
    Chinese Journal of Oceanology and Limnology, 2014
    Co-Authors: Yuangao Deng, Patrick Sorgeloos, Jing Wang, Gilbert Van Stappen

    Abstract:

    We studied the effect of pH (pH 5, 6, 7 and 8) on the hatching percentage, survival and reproduction of Artemia strains in Bohai Bay salt ponds. Strains included parthenogenetic Artemia from Bohai Bay (BHB), Artemia franciscana from San Francisco Bay, and A. franciscana artificially produced in salt ponds in Vietnam. The latter was included as a potential inoculum for biological management of salt ponds. The hatching percentage of cysts after 24 h and the survival rate of the tested Artemia strains were significantly reduced when exposed to a culture medium at pH 5 for 18 d (P

  • Morphological characteristics of the digestive tract of gnotobiotic Artemia franciscana nauplii
    Aquaculture, 2011
    Co-Authors: R.a.y.s. Asanka Gunasekara, Peter Bossier, Patrick Sorgeloos, Anamaria Rekecki, Pieter Cornillie, Maria Cornelissen, Paul Simoens, Wim Van Den Broeck

    Abstract:

    Abstract Cysts of Artemia franciscana were hatched and nauplii were reared under gnotobiotic conditions (gnotobiotic Artemia rearing system). Stereomicroscopy, computer assisted three-dimensional reconstruction, light microscopy, and transmission electron microscopy were used to study the structural and cellular morphology of their digestive tracts. The alimentary tract of gnotobiotic Artemia nauplii, fed with dead Aeromonas hydrophila and wild type strain of Saccharomyces cerevisiae , is a hooked, tubular structure which is composed of three clearly distinguishable parts, i.e. the foregut, midgut and hindgut that are freely suspended in haemolymph. The epithelium lining of the entire gut consists of a single cell layer. Enterocytes of the foregut and hindgut are cuboidal and lined by a thin cuticle, whereas midgut enterocytes are cuboidal to columnar and possess an apical brush border. The fore- and hindgut mainly display characteristics suggestive for mechanical functions, whereas the midgut shows characteristics of absorption, storage and secretion. The gnotobiotic Artemia rearing system is most useful to investigate the effects of micro-organisms on the development of nauplii. The knowledge acquired in this study potentially facilitates the evaluation of gut morphology when specific micro-organisms are introduced into the culture system, as compared to the gnotobiotic counterparts.

  • short chain fatty acids protect gnotobiotic Artemia franciscana from pathogenic vibrio campbellii
    Aquaculture, 2006
    Co-Authors: Tom Defoirdt, Peter Bossier, Patrick Sorgeloos, Dirk Halet, Willy Verstraete

    Abstract:

    Infections caused by antibiotic resistant luminescent vibrios can cause considerable losses in aquaculture. In this study, different short-chain fatty acids were investigated as possible alternative biocontrol agents. The addition of 100 mM formic, acetic, propionic, butyric or valeric acid to the growth medium of a pathogenic Vibrio campbellii strain completely inhibited its growth at pH 6. At 10 mM, the growth of the pathogen was delayed, whereas at 1 mM, no effect could be observed. The growth-inhibitory effect was clearly pH-dependent and decreased with increasing pH. An in vivo challenge test with gnotobiotic Artemia franciscana nauplii revealed that all five short-chain fatty acids protected the shrimp from the pathogenic V. campbellii strain. The addition of 20 mM of the short-chain fatty acids to the culture water resulted in a significantly increased survival of infected nauplii, with no difference between the different fatty acids. In conclusion, our data indicate that short-chain fatty acids might be useful as alternative biocontrol agents to treat luminescent vibriosis