Ascorbic Acid Deficiency

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Fumihiko Horio - One of the best experts on this subject based on the ideXlab platform.

  • Ascorbic Acid Deficiency decreases hepatic cytochrome p 450 especially cyp2b1 2b2 and simultaneously induces heme oxygenase 1 gene expression in scurvy prone ods rats
    Bioscience Biotechnology and Biochemistry, 2014
    Co-Authors: Misato Kobayashi, Yukiko Hoshinaga, Natsuko Miura, Yuki Tokuda, Shigeru Shigeoka, Atsushi Murai, Fumihiko Horio
    Abstract:

    The mechanisms underlying the decrease in hepatic cytochrome P-450 (CYP) content in Ascorbic Acid Deficiency was investigated in scurvy-prone ODS rats. First, male ODS rats were fed a diet containing sufficient Ascorbic Acid (control) or a diet without Ascorbic Acid (deficient) for 18 days, with or without the intraperitoneal injection of phenobarbital. Ascorbic Acid Deficiency decreased hepatic microsomal total CYP content, CYP2B1/2B2 protein, and mitochondrial cytochrome oxidase (COX) complex IV subunit I protein, and simultaneously increased heme oxygenase-1 protein in microsomes and mitochondria. Next, heme oxygenase-1 inducers, that is lipopolysaccharide and hemin, were administered to phenobaribital-treated ODS rats fed sufficient Ascorbic Acid. The administration of these inducers decreased hepatic microsomal total CYP content, CYP2B1/2B2 protein, and mitochondrial COX complex IV subunit I protein. These results suggested that the stimulation of hepatic heme oxygenase-1 expression by Ascorbic Acid ...

  • Ascorbic Acid Deficiency decreases hepatic cytochrome P-450, especially CYP2B1/2B2, and simultaneously induces heme oxygenase-1 gene expression in scurvy-prone ODS rats
    Bioscience biotechnology and biochemistry, 2014
    Co-Authors: Misato Kobayashi, Yukiko Hoshinaga, Natsuko Miura, Yuki Tokuda, Shigeru Shigeoka, Atsushi Murai, Fumihiko Horio
    Abstract:

    The mechanisms underlying the decrease in hepatic cytochrome P-450 (CYP) content in Ascorbic Acid Deficiency was investigated in scurvy-prone ODS rats. First, male ODS rats were fed a diet containing sufficient Ascorbic Acid (control) or a diet without Ascorbic Acid (deficient) for 18 days, with or without the intraperitoneal injection of phenobarbital. Ascorbic Acid Deficiency decreased hepatic microsomal total CYP content, CYP2B1/2B2 protein, and mitochondrial cytochrome oxidase (COX) complex IV subunit I protein, and simultaneously increased heme oxygenase-1 protein in microsomes and mitochondria. Next, heme oxygenase-1 inducers, that is lipopolysaccharide and hemin, were administered to phenobaribital-treated ODS rats fed sufficient Ascorbic Acid. The administration of these inducers decreased hepatic microsomal total CYP content, CYP2B1/2B2 protein, and mitochondrial COX complex IV subunit I protein. These results suggested that the stimulation of hepatic heme oxygenase-1 expression by Ascorbic Acid ...

  • Ascorbic Acid Deficiency stimulates hepatic expression of inflammatory chemokine, cytokine-induced neutrophil chemoattractant-1, in scurvy-prone ODS rats.
    Journal of nutritional science and vitaminology, 2006
    Co-Authors: Fumihiko Horio, Keiichiro Kiyama, Misato Kobayashi, Kaori Kawai, Takanori Tsuda
    Abstract:

    ODS rat has a hereditary defect in Ascorbic Acid biosynthesis and is a useful animal model for elucidating the physiological role of Ascorbic Acid. We previously demonstrated by using ODS rats that Ascorbic Acid Deficiency changes the hepatic gene expression of acute phase proteins, as seen in acute inflammation. In this study, we investigated the effects of Ascorbic Acid Deficiency on the production of inflammatory chemokine, cytokine-induced neutrophil chemoattractant-1 (CINC-1), in ODS rats. Male ODS rats (6 wk of age) were fed a basal diet containing Ascorbic Acid (300 mg/kg diet) or a diet without Ascorbic Acid for 14 d. Obvious symptoms of scurvy were not observed in the Ascorbic Acid-deficient rats. Ascorbic Acid Deficiency significantly elevated the serum concentration of CINC-1 on d 14. The liver and spleen CINC-1 concentrations in the Ascorbic Acid-deficient rats were significantly elevated to 600% and 180% of the respective values in the control rats. However, the lung concentration of CINC-1 was not affected by Ascorbic Acid Deficiency. Ascorbic Acid Deficiency significantly elevated the hepatic mRNA level of CINC-1 (to 480% of the value in the control rats), but not the lung mRNA level. These results demonstrate that Ascorbic Acid Deficiency elevates the serum, liver and spleen concentrations of CINC-1 as seen in acute inflammation, and suggest that Ascorbic Acid Deficiency stimulate the hepatic CINC-1 gene expression.

  • A newly established strain of spontaneously hypertensive rat with a defect of Ascorbic Acid biosynthesis.
    Life sciences, 2001
    Co-Authors: Fumihiko Horio, Susumu Makino, Atsushi Kakinuma, Kaori Hayashi, Takashi Mishima, Kumiko Takemori, Itsuki Oshima, Hiroyuki Ito
    Abstract:

    Abstract To investigate the effects of Ascorbic Acid Deficiency on the pathogenesis of hypertension and/or its complications, we established a rat strain with both genetic hypertension and a defect of Ascorbic Acid biosynthesis. The od gene (L-gulono-γ-lactone oxidase gene) of the ODS (Osteogenic Disorder Shionogi) rat, which is a rat mutant unable to synthesize Ascorbic Acid, was introduced into spontaneously hypertensive rats (SHR), and a novel congenic strain, SHR-od, was established. SHR-od showed scurvy when fed an Ascorbic Acid-free diet. Systolic blood pressure of male SHR-od began to increase at 9 weeks of age and reached 190–200 mmHg at 20 weeks of age. In 25-week-old SHR-od, Ascorbic Acid Deficiency when fed an Ascorbic Acid-free diet for 6 weeks caused a remarkable reduction of blood pressure to lower than 110 mmHg. The wall to lumen ratio of the testicular artery in Ascorbic Acid-deficient SHR-od was lower than that of the control rats. When rats were fed a diet supplemented with Ascorbic Acid (300 mg/kg), Ascorbic Acid concentration in SHR-od was lower in the serum and liver than that in ODS rats. These results indicate that Ascorbic Acid could be closely related to the development of hypertension in SHR-od. We believe that SHR-od will be a useful model for experimental studies on hypertension and its complications, since all of them suffer from hypertension spontaneously and the level of Ascorbic Acid Deficiency in these rats could be controlled at will both in concentration and duration.

  • Ascorbic Acid Deficiency changes hepatic gene expression of acute phase proteins in scurvy-prone ODS rats
    The Journal of nutrition, 1998
    Co-Authors: Saiko Ikeda, Fumihiko Horio, Atsushi Kakinuma
    Abstract:

    The ODS rat (genotype od/od), which has a hereditary defect in Ascorbic Acid biosynthesis, was used to investigate the effects of Ascorbic Acid Deficiency on the hepatic gene expression of both the positive acute phase proteins, haptoglobin and alpha1-Acid glycoprotein, and the negative acute phase proteins, apolipoprotein A-I and albumin. Male ODS rats (6 wk old, body weight approximately 140 g) were fed a basal diet containing Ascorbic Acid (300 mg/kg diet) or a diet without Ascorbic Acid for 14 d. Ascorbic Acid Deficiency significantly elevated the serum concentration of haptoglobin and significantly lowered those of apolipoprotein A-I and albumin. The hepatic mRNA levels of haptoglobin and alpha1-Acid glycoprotein in the Ascorbic Acid-deficient rats were significantly elevated on d 12, and reached 260 (P < 0.05) and 360% (P < 0.01) of respective values in the control rats on d 14. On the contrary, the hepatic mRNA levels of apolipoprotein A-I and albumin in the Ascorbic Acid-deficient rats were lowered to 68 (P < 0.01) and 71% (P < 0.05) of respective values in the control rats on d 14. Although Ascorbic Acid Deficiency significantly elevated the serum corticosterone concentration on d 14, the changes in mRNA levels of haptoglobin, alpha1-Acid glycoprotein, apolipoprotein A-I and albumin due to Ascorbic Acid Deficiency were not affected by adrenalectomy, as assessed in a separate experiment. The serum concentration of interleukin-6, an inflammatory cytokine that stimulates gene expression of some acute phase proteins, was significantly higher in the Ascorbic Acid-deficient rats on d 14 than in the control rats. These results suggest that Ascorbic Acid Deficiency causes physiologic changes similar to those that occur in the acute phase response.

Yoshiji Ohta - One of the best experts on this subject based on the ideXlab platform.

  • Short-term Ascorbic Acid Deficiency induced oxidative stress in the retinas of young guinea pigs
    Journal of Biomedical Science, 2004
    Co-Authors: Yoshiji Ohta, Takafumi Niwa, Takashi Yamasaki, Taku Okubo, Masayuki Horiguchi
    Abstract:

    We examined whether short-term Ascorbic Acid Deficiency induces oxidative stress in the retinas of young guinea pigs. Four-week-old guinea pigs were given a scorbutic diet (20 g/animal/day) with and without adequate Ascorbic Acid (400 mg/animal/day) in drinking water for 3 weeks. The serum concentrations of the reduced form of Ascorbic Acid and the oxidized form of Ascorbic Acid in the deficient group were 14.1 and 4.1%, respectively, of those in the adequate group. The retinal contents of the reduced form of Ascorbic Acid and the oxidized form of Ascorbic Acid in the deficient group were 6.4 and 27.3%, respectively, of those in the adequate group. The retinal content of thiobarbituric Acid-reactive substances, an index of lipid peroxidation, was 1.9-fold higher in the deficient group than in the adequate group. Retinal reduced glutathione and vitamin E contents in the deficient group were 70.1 and 69.4%, respectively, of those in the adequate group. This Ascorbic Acid Deficiency did not affect serum thiobarbituric Acid-reactive substances and reduced glutathione concentrations but increased serum vitamin E concentration. These results indicate that short-term Ascorbic Acid Deficiency induces oxidative stress in the retinas of young guinea pigs without disrupting systemic antioxidant status.

  • Short-term Ascorbic Acid Deficiency does not impair antioxidant status in lens of guinea pigs.
    Journal of nutritional science and vitaminology, 2004
    Co-Authors: Yoshiji Ohta, Takafumi Niwa, Takashi Yamasaki
    Abstract:

    We examined whether short-term Ascorbic Acid Deficiency impairs antioxidant status in the lens of guinea pigs. Male guinea pigs aged 4 wk were given a scorbutic diet (20 g/animal per day) with and without Ascorbic Acid (400 mg/animal per day) in drinking water for 3 wk. The Ascorbic Acid-deficient group showed no lens opacity. The Ascorbic Acid-deficient group had 14% of serum Ascorbic Acid concentration, 6% of aqueous humor Ascorbic Acid concentration, and 18% of lens Ascorbic Acid content in the Ascorbic Acid-adequate group. There were no differences in the contents of lens reduced glutathione and thiobarbituric Acid reactive substances, an index of lipid peroxidation, between the Ascorbic Acid-deficient and adequate groups, while the deficient group had higher lens vitamin E content than the adequate group. The Ascorbic Acid-deficient group had higher serum vitamin E concentration than the Ascorbic Acid adequate group, while there were no differences in the concentrations of serum reduced glutathione and tiobarbituric Acid reactive substances between the deficient and adequate groups. These results indicate that short-term Ascorbic Acid Deficiency does not impair antioxidant status in the lens of guinea pigs despite induction of severe Ascorbic Acid depletion in the tissue, which may result in no cataract formation.

  • Prolonged marginal Ascorbic Acid Deficiency induces oxidative stress in retina of guinea pigs.
    International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vi, 2002
    Co-Authors: Yoshiji Ohta, Takafumi Niwa, Takashi Yamasaki, Taku Okubo, Masayuki Horiguchi
    Abstract:

    We examined whether prolonged marginal Ascorbic Acid Deficiency induces oxidative stress in the retina of guinea pigs. Male guinea pigs aged four weeks were given a scorbutic diet (20 g/animal per day) with either marginally deficient Ascorbic Acid (0.5 mg/animal per day) or adequate Ascorbic Acid (1 g/animal per day) in drinking water for three and six months. The retinal contents of the reduced form of Ascorbic Acid in the deficient group at three and six months were 68.1 and 43.5%, respectively, of that in the corresponding adequate group. The retinal contents of the oxidized form of Ascorbic Acid in the deficient group at three and six months were 1.9- and 2.7-fold, respectively, higher than that in the corresponding adequate group. The content of retinal thiobarbituric Acid reactive substances (TBARS), an index of lipid peroxidation, in the deficient group was 2.5-fold higher than that in the adequate group at six months. The retinal contents of reduced glutathione (GSH) in the deficient group at three and six months were 84.8 and 66.7%, respectively, of that in the corresponding adequate group. The deficient group had 37.5% of retinal vitamin E content of the adequate group at six months. The deficient group had higher serum vitamin E concentration than the adequate group in both experimental periods. There were no differences in serum TBARS and GSH concentrations between the groups at both periods. These results indicate that prolonged marginal Ascorbic Acid Deficiency induces oxidative stress in the retina of guinea pigs without systemic oxidative stress.

  • Effect of prolonged marginal Ascorbic Acid Deficiency on lenticular levels of antioxidants and lipid peroxide in guinea pigs.
    International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vi, 2001
    Co-Authors: Yoshiji Ohta, Takafumi Niwa, Takashi Yamasaki
    Abstract:

    We examined the effect of prolonged marginal Ascorbic Acid Deficiency of the levels of antioxidants and lipid peroxide in lenses of guinea pigs in order to clarify lenticular antioxidant status under Ascorbic Acid Deficiency. Male guinea pigs aged 4 weeks were given a scorbutic diet (20 g/animal per day) with either marginally deficient Ascorbic Acid (0.5 mg/animal per day) or sufficient Ascorbic Acid (1 g/animal per day) in drinking water for 3 and 6 months. The deficient group showed no lens opacity during the administration period. The deficient group had 62.3 and 53.9% of lenticular Ascorbic Acid content in the sufficient group at 3 and 6 months of Ascorbic Acid Deficiency, respectively. There were no differences in lenticular contents of reduced glutathione and thiobarbituric Acid reactive substances, an index of lipid peroxidation, between both groups at 3 and 6 months of Ascorbic Acid Deficiency, while the deficient group tended to have higher lenticular vitamin E content than the sufficient group. The deficient group had higher serum vitamin E concentration than the sufficient group at 3 and 6 months of Ascorbic Acid Deficiency. These results indicate that lenticular antioxidant status is maintained well in guinea pigs with prolonged marginal Ascorbic Acid Deficiency, which may result in no cataract formation.

Masayuki Horiguchi - One of the best experts on this subject based on the ideXlab platform.

  • Short-term Ascorbic Acid Deficiency induced oxidative stress in the retinas of young guinea pigs
    Journal of Biomedical Science, 2004
    Co-Authors: Yoshiji Ohta, Takafumi Niwa, Takashi Yamasaki, Taku Okubo, Masayuki Horiguchi
    Abstract:

    We examined whether short-term Ascorbic Acid Deficiency induces oxidative stress in the retinas of young guinea pigs. Four-week-old guinea pigs were given a scorbutic diet (20 g/animal/day) with and without adequate Ascorbic Acid (400 mg/animal/day) in drinking water for 3 weeks. The serum concentrations of the reduced form of Ascorbic Acid and the oxidized form of Ascorbic Acid in the deficient group were 14.1 and 4.1%, respectively, of those in the adequate group. The retinal contents of the reduced form of Ascorbic Acid and the oxidized form of Ascorbic Acid in the deficient group were 6.4 and 27.3%, respectively, of those in the adequate group. The retinal content of thiobarbituric Acid-reactive substances, an index of lipid peroxidation, was 1.9-fold higher in the deficient group than in the adequate group. Retinal reduced glutathione and vitamin E contents in the deficient group were 70.1 and 69.4%, respectively, of those in the adequate group. This Ascorbic Acid Deficiency did not affect serum thiobarbituric Acid-reactive substances and reduced glutathione concentrations but increased serum vitamin E concentration. These results indicate that short-term Ascorbic Acid Deficiency induces oxidative stress in the retinas of young guinea pigs without disrupting systemic antioxidant status.

  • Prolonged marginal Ascorbic Acid Deficiency induces oxidative stress in retina of guinea pigs.
    International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vi, 2002
    Co-Authors: Yoshiji Ohta, Takafumi Niwa, Takashi Yamasaki, Taku Okubo, Masayuki Horiguchi
    Abstract:

    We examined whether prolonged marginal Ascorbic Acid Deficiency induces oxidative stress in the retina of guinea pigs. Male guinea pigs aged four weeks were given a scorbutic diet (20 g/animal per day) with either marginally deficient Ascorbic Acid (0.5 mg/animal per day) or adequate Ascorbic Acid (1 g/animal per day) in drinking water for three and six months. The retinal contents of the reduced form of Ascorbic Acid in the deficient group at three and six months were 68.1 and 43.5%, respectively, of that in the corresponding adequate group. The retinal contents of the oxidized form of Ascorbic Acid in the deficient group at three and six months were 1.9- and 2.7-fold, respectively, higher than that in the corresponding adequate group. The content of retinal thiobarbituric Acid reactive substances (TBARS), an index of lipid peroxidation, in the deficient group was 2.5-fold higher than that in the adequate group at six months. The retinal contents of reduced glutathione (GSH) in the deficient group at three and six months were 84.8 and 66.7%, respectively, of that in the corresponding adequate group. The deficient group had 37.5% of retinal vitamin E content of the adequate group at six months. The deficient group had higher serum vitamin E concentration than the adequate group in both experimental periods. There were no differences in serum TBARS and GSH concentrations between the groups at both periods. These results indicate that prolonged marginal Ascorbic Acid Deficiency induces oxidative stress in the retina of guinea pigs without systemic oxidative stress.

Takashi Yamasaki - One of the best experts on this subject based on the ideXlab platform.

  • Short-term Ascorbic Acid Deficiency induced oxidative stress in the retinas of young guinea pigs
    Journal of Biomedical Science, 2004
    Co-Authors: Yoshiji Ohta, Takafumi Niwa, Takashi Yamasaki, Taku Okubo, Masayuki Horiguchi
    Abstract:

    We examined whether short-term Ascorbic Acid Deficiency induces oxidative stress in the retinas of young guinea pigs. Four-week-old guinea pigs were given a scorbutic diet (20 g/animal/day) with and without adequate Ascorbic Acid (400 mg/animal/day) in drinking water for 3 weeks. The serum concentrations of the reduced form of Ascorbic Acid and the oxidized form of Ascorbic Acid in the deficient group were 14.1 and 4.1%, respectively, of those in the adequate group. The retinal contents of the reduced form of Ascorbic Acid and the oxidized form of Ascorbic Acid in the deficient group were 6.4 and 27.3%, respectively, of those in the adequate group. The retinal content of thiobarbituric Acid-reactive substances, an index of lipid peroxidation, was 1.9-fold higher in the deficient group than in the adequate group. Retinal reduced glutathione and vitamin E contents in the deficient group were 70.1 and 69.4%, respectively, of those in the adequate group. This Ascorbic Acid Deficiency did not affect serum thiobarbituric Acid-reactive substances and reduced glutathione concentrations but increased serum vitamin E concentration. These results indicate that short-term Ascorbic Acid Deficiency induces oxidative stress in the retinas of young guinea pigs without disrupting systemic antioxidant status.

  • Short-term Ascorbic Acid Deficiency does not impair antioxidant status in lens of guinea pigs.
    Journal of nutritional science and vitaminology, 2004
    Co-Authors: Yoshiji Ohta, Takafumi Niwa, Takashi Yamasaki
    Abstract:

    We examined whether short-term Ascorbic Acid Deficiency impairs antioxidant status in the lens of guinea pigs. Male guinea pigs aged 4 wk were given a scorbutic diet (20 g/animal per day) with and without Ascorbic Acid (400 mg/animal per day) in drinking water for 3 wk. The Ascorbic Acid-deficient group showed no lens opacity. The Ascorbic Acid-deficient group had 14% of serum Ascorbic Acid concentration, 6% of aqueous humor Ascorbic Acid concentration, and 18% of lens Ascorbic Acid content in the Ascorbic Acid-adequate group. There were no differences in the contents of lens reduced glutathione and thiobarbituric Acid reactive substances, an index of lipid peroxidation, between the Ascorbic Acid-deficient and adequate groups, while the deficient group had higher lens vitamin E content than the adequate group. The Ascorbic Acid-deficient group had higher serum vitamin E concentration than the Ascorbic Acid adequate group, while there were no differences in the concentrations of serum reduced glutathione and tiobarbituric Acid reactive substances between the deficient and adequate groups. These results indicate that short-term Ascorbic Acid Deficiency does not impair antioxidant status in the lens of guinea pigs despite induction of severe Ascorbic Acid depletion in the tissue, which may result in no cataract formation.

  • Prolonged marginal Ascorbic Acid Deficiency induces oxidative stress in retina of guinea pigs.
    International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vi, 2002
    Co-Authors: Yoshiji Ohta, Takafumi Niwa, Takashi Yamasaki, Taku Okubo, Masayuki Horiguchi
    Abstract:

    We examined whether prolonged marginal Ascorbic Acid Deficiency induces oxidative stress in the retina of guinea pigs. Male guinea pigs aged four weeks were given a scorbutic diet (20 g/animal per day) with either marginally deficient Ascorbic Acid (0.5 mg/animal per day) or adequate Ascorbic Acid (1 g/animal per day) in drinking water for three and six months. The retinal contents of the reduced form of Ascorbic Acid in the deficient group at three and six months were 68.1 and 43.5%, respectively, of that in the corresponding adequate group. The retinal contents of the oxidized form of Ascorbic Acid in the deficient group at three and six months were 1.9- and 2.7-fold, respectively, higher than that in the corresponding adequate group. The content of retinal thiobarbituric Acid reactive substances (TBARS), an index of lipid peroxidation, in the deficient group was 2.5-fold higher than that in the adequate group at six months. The retinal contents of reduced glutathione (GSH) in the deficient group at three and six months were 84.8 and 66.7%, respectively, of that in the corresponding adequate group. The deficient group had 37.5% of retinal vitamin E content of the adequate group at six months. The deficient group had higher serum vitamin E concentration than the adequate group in both experimental periods. There were no differences in serum TBARS and GSH concentrations between the groups at both periods. These results indicate that prolonged marginal Ascorbic Acid Deficiency induces oxidative stress in the retina of guinea pigs without systemic oxidative stress.

  • Effect of prolonged marginal Ascorbic Acid Deficiency on lenticular levels of antioxidants and lipid peroxide in guinea pigs.
    International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vi, 2001
    Co-Authors: Yoshiji Ohta, Takafumi Niwa, Takashi Yamasaki
    Abstract:

    We examined the effect of prolonged marginal Ascorbic Acid Deficiency of the levels of antioxidants and lipid peroxide in lenses of guinea pigs in order to clarify lenticular antioxidant status under Ascorbic Acid Deficiency. Male guinea pigs aged 4 weeks were given a scorbutic diet (20 g/animal per day) with either marginally deficient Ascorbic Acid (0.5 mg/animal per day) or sufficient Ascorbic Acid (1 g/animal per day) in drinking water for 3 and 6 months. The deficient group showed no lens opacity during the administration period. The deficient group had 62.3 and 53.9% of lenticular Ascorbic Acid content in the sufficient group at 3 and 6 months of Ascorbic Acid Deficiency, respectively. There were no differences in lenticular contents of reduced glutathione and thiobarbituric Acid reactive substances, an index of lipid peroxidation, between both groups at 3 and 6 months of Ascorbic Acid Deficiency, while the deficient group tended to have higher lenticular vitamin E content than the sufficient group. The deficient group had higher serum vitamin E concentration than the sufficient group at 3 and 6 months of Ascorbic Acid Deficiency. These results indicate that lenticular antioxidant status is maintained well in guinea pigs with prolonged marginal Ascorbic Acid Deficiency, which may result in no cataract formation.

Takafumi Niwa - One of the best experts on this subject based on the ideXlab platform.

  • Short-term Ascorbic Acid Deficiency induced oxidative stress in the retinas of young guinea pigs
    Journal of Biomedical Science, 2004
    Co-Authors: Yoshiji Ohta, Takafumi Niwa, Takashi Yamasaki, Taku Okubo, Masayuki Horiguchi
    Abstract:

    We examined whether short-term Ascorbic Acid Deficiency induces oxidative stress in the retinas of young guinea pigs. Four-week-old guinea pigs were given a scorbutic diet (20 g/animal/day) with and without adequate Ascorbic Acid (400 mg/animal/day) in drinking water for 3 weeks. The serum concentrations of the reduced form of Ascorbic Acid and the oxidized form of Ascorbic Acid in the deficient group were 14.1 and 4.1%, respectively, of those in the adequate group. The retinal contents of the reduced form of Ascorbic Acid and the oxidized form of Ascorbic Acid in the deficient group were 6.4 and 27.3%, respectively, of those in the adequate group. The retinal content of thiobarbituric Acid-reactive substances, an index of lipid peroxidation, was 1.9-fold higher in the deficient group than in the adequate group. Retinal reduced glutathione and vitamin E contents in the deficient group were 70.1 and 69.4%, respectively, of those in the adequate group. This Ascorbic Acid Deficiency did not affect serum thiobarbituric Acid-reactive substances and reduced glutathione concentrations but increased serum vitamin E concentration. These results indicate that short-term Ascorbic Acid Deficiency induces oxidative stress in the retinas of young guinea pigs without disrupting systemic antioxidant status.

  • Short-term Ascorbic Acid Deficiency does not impair antioxidant status in lens of guinea pigs.
    Journal of nutritional science and vitaminology, 2004
    Co-Authors: Yoshiji Ohta, Takafumi Niwa, Takashi Yamasaki
    Abstract:

    We examined whether short-term Ascorbic Acid Deficiency impairs antioxidant status in the lens of guinea pigs. Male guinea pigs aged 4 wk were given a scorbutic diet (20 g/animal per day) with and without Ascorbic Acid (400 mg/animal per day) in drinking water for 3 wk. The Ascorbic Acid-deficient group showed no lens opacity. The Ascorbic Acid-deficient group had 14% of serum Ascorbic Acid concentration, 6% of aqueous humor Ascorbic Acid concentration, and 18% of lens Ascorbic Acid content in the Ascorbic Acid-adequate group. There were no differences in the contents of lens reduced glutathione and thiobarbituric Acid reactive substances, an index of lipid peroxidation, between the Ascorbic Acid-deficient and adequate groups, while the deficient group had higher lens vitamin E content than the adequate group. The Ascorbic Acid-deficient group had higher serum vitamin E concentration than the Ascorbic Acid adequate group, while there were no differences in the concentrations of serum reduced glutathione and tiobarbituric Acid reactive substances between the deficient and adequate groups. These results indicate that short-term Ascorbic Acid Deficiency does not impair antioxidant status in the lens of guinea pigs despite induction of severe Ascorbic Acid depletion in the tissue, which may result in no cataract formation.

  • Prolonged marginal Ascorbic Acid Deficiency induces oxidative stress in retina of guinea pigs.
    International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vi, 2002
    Co-Authors: Yoshiji Ohta, Takafumi Niwa, Takashi Yamasaki, Taku Okubo, Masayuki Horiguchi
    Abstract:

    We examined whether prolonged marginal Ascorbic Acid Deficiency induces oxidative stress in the retina of guinea pigs. Male guinea pigs aged four weeks were given a scorbutic diet (20 g/animal per day) with either marginally deficient Ascorbic Acid (0.5 mg/animal per day) or adequate Ascorbic Acid (1 g/animal per day) in drinking water for three and six months. The retinal contents of the reduced form of Ascorbic Acid in the deficient group at three and six months were 68.1 and 43.5%, respectively, of that in the corresponding adequate group. The retinal contents of the oxidized form of Ascorbic Acid in the deficient group at three and six months were 1.9- and 2.7-fold, respectively, higher than that in the corresponding adequate group. The content of retinal thiobarbituric Acid reactive substances (TBARS), an index of lipid peroxidation, in the deficient group was 2.5-fold higher than that in the adequate group at six months. The retinal contents of reduced glutathione (GSH) in the deficient group at three and six months were 84.8 and 66.7%, respectively, of that in the corresponding adequate group. The deficient group had 37.5% of retinal vitamin E content of the adequate group at six months. The deficient group had higher serum vitamin E concentration than the adequate group in both experimental periods. There were no differences in serum TBARS and GSH concentrations between the groups at both periods. These results indicate that prolonged marginal Ascorbic Acid Deficiency induces oxidative stress in the retina of guinea pigs without systemic oxidative stress.

  • Effect of prolonged marginal Ascorbic Acid Deficiency on lenticular levels of antioxidants and lipid peroxide in guinea pigs.
    International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vi, 2001
    Co-Authors: Yoshiji Ohta, Takafumi Niwa, Takashi Yamasaki
    Abstract:

    We examined the effect of prolonged marginal Ascorbic Acid Deficiency of the levels of antioxidants and lipid peroxide in lenses of guinea pigs in order to clarify lenticular antioxidant status under Ascorbic Acid Deficiency. Male guinea pigs aged 4 weeks were given a scorbutic diet (20 g/animal per day) with either marginally deficient Ascorbic Acid (0.5 mg/animal per day) or sufficient Ascorbic Acid (1 g/animal per day) in drinking water for 3 and 6 months. The deficient group showed no lens opacity during the administration period. The deficient group had 62.3 and 53.9% of lenticular Ascorbic Acid content in the sufficient group at 3 and 6 months of Ascorbic Acid Deficiency, respectively. There were no differences in lenticular contents of reduced glutathione and thiobarbituric Acid reactive substances, an index of lipid peroxidation, between both groups at 3 and 6 months of Ascorbic Acid Deficiency, while the deficient group tended to have higher lenticular vitamin E content than the sufficient group. The deficient group had higher serum vitamin E concentration than the sufficient group at 3 and 6 months of Ascorbic Acid Deficiency. These results indicate that lenticular antioxidant status is maintained well in guinea pigs with prolonged marginal Ascorbic Acid Deficiency, which may result in no cataract formation.