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J. A. Teixeira - One of the best experts on this subject based on the ideXlab platform.
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Fermentation pH in stirred tank and air-lift bioreactors affects phytase secretion by Aspergillus japonicus differently but not the particle size
Biocatalysis and Biotransformation, 2014Co-Authors: Alexandre Maller, J. A. Teixeira, André Mota, Daniel Pereira Da Silva, António A. Vicente, Maria De Lourdes Teixeira De Moraes PolizeliAbstract:AbstractPhytases are mainly produced by filamentous fungi and have great potential for biotechnological use in animal feed treatment, because this enzyme hydrolyzes ester bonds of the phytic acid releasing inositol and inorganic phosphate. The aim of this work was to evaluate the effect of pH on the production of phytase by Aspergillus japonicus in two different bioreactors, known to have different mixing patterns—stirred tank and air-lift bioreactors. The maximum phytase production—53 U/mL—was obtained at 120 h in the stirred tank while in the air-lift the maximum value was 41 U/mL, observed at 144 h. In fermentations evaluated at controlled pH values (3.5, 6.0, and 7.5), the stirred tank was more efficient for production of phytase than the air-lift. Under these conditions, the highest value was measured at 24 h and pH 3.5. These results were not closely related to fungi particle size, because hyphae with a similar diameter (0.51–0.63 mm) and sphericity (0.78–0.87 mm) secreted different amounts of phyta...
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maximization of fructooligosaccharides and β fructofuranosidase production by Aspergillus japonicus under solid state fermentation conditions
Food and Bioprocess Technology, 2013Co-Authors: Solange Inês Mussatto, Lina F Ballesteros, Silvia Martins, Cristobal N Aguilar, Dulce A F Maltos, J. A. TeixeiraAbstract:The conditions of temperature, moisture content, and inoculum rate able to maximize the production of fructooligosaccharides (FOS) and β-fructofuranosidase (FFase) enzyme by solid-state fermentation were established. Fermentation assays were performed using the support material (coffee silverskin) moistened to 60, 70, or 80 % with a 240-g/l sucrose solution and inoculated with a spore suspension of Aspergillus japonicus to obtain 2 × 105, 2 × 106, or 2 × 107 spores/gram dry material. The fermentation runs were maintained under static conditions at 26, 30, or 34 °C during 20 h. The moisture content did not influence the FOS and FFase production; however, temperature between 26 and 30 °C and inoculum rate of approximately 2 × 107 spores/gram dry material maximized the results (FOS = 208.8 g/l with productivity of 10.44 g/l h; FFase = 64.12 units U/ml with productivity of 4.0 U/ml h). These results are considerably higher than those obtained under no optimized fermentation conditions and represent an important contribution for the establishment of a new industrial process for FOS and FFase production.
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process conditions that maximize the fructooligosaccharides and β fructofuranosidase production by Aspergillus japonicus under solid state fermentation conditions
XIV Congreso Nacional de Biotecnología y Bioingeniería, 2011Co-Authors: Solange Inês Mussatto, Lina F Ballesteros, Silvia Martins, Dulce A Floresmaltos, Cristobal N Aguilar, J. A. TeixeiraAbstract:Introduction. Several factors must be considered for the development of a successful bioprocess under solid-state fermentation (SSF) conditions. Some of them include the selection of the solid support, and determination of the operational conditions to be used. In a recent study, coffee silverskin (CS) was demonstrated to be a material with great potential for use as support and nutrient source for fructooligosaccharides (FOS) and β-fructofuranosidase (FFase) production by Aspergillus japonicus under SSF conditions (1).
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increase in the fructooligosaccharides yield and productivity by solid state fermentation with Aspergillus japonicus using agro industrial residues as support and nutrient source
Biochemical Engineering Journal, 2010Co-Authors: Solange Inês Mussatto, J. A. TeixeiraAbstract:Corn cobs, coffee silverskin, and cork oak were used as support and nutrient source during the fructooligosaccharides (FOS) production by Aspergillus japonicus, under solid-state fermentation (SSF) conditions. The objectives of this study consisted in evaluating the possibility of improving the FOS yield and productivity, besides to finding an alternative to reduce the production costs, and add value to these agro-industrial residues. Fermentation assays were performed by using the materials as solid support, supplemented or not with nutrients. For comparison, assays were also performed using a synthetic material as solid support, under the same operational conditions. All the material residues acted as nutrient source for the microorganism, since FOS production occurred when all of them were used without nutrient supplementation, but not when the synthetic material was used. Among the evaluated materials, coffee silverskin gave the most interesting fermentation results, with a FOS production similar in both supplemented and non-supplemented media. The elevated FOS production (128.7 g/l) and -fructofuranosidase activity (71.3 U/ml) obtained by using this material suggest SSF of coffee silverskin with A. japonicus as an interesting and promising strategy to synthesize both products at the industrial level.
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fructooligosaccharides and β fructofuranosidase production by Aspergillus japonicus immobilized on lignocellulosic materials
Journal of Molecular Catalysis B-enzymatic, 2009Co-Authors: Solange Inês Mussatto, Lígia R. Rodrigues, Cristobal N Aguilar, J. A. TeixeiraAbstract:Abstract This work describes the fructooligosaccharides (FOS) and β-fructofuranosidase (FFase) production from sucrose (200 g/l) by Aspergillus japonicus ATCC 20236 immobilized on different lignocellulosic materials including brewer's spent grain, wheat straw, corn cobs, coffee husks, cork oak, and loofa sponge. Transfructosylating (U t ) and hydrolyzing (U h ) activities of FFase were also determined. The FOS production and FFase activity ranged from 128.35 to 138.73 g/l, and 26.83 to 44.81 U/ml, respectively, for cells immobilized in the different carriers. Corn cobs was the best support material since gave the highest results of microorganism immobilization (1.49 g/g carrier), FOS and FFase production, with FOS productivity (6.61 g/l h) and yield (0.66 g/g based on total substrate; 0.73 g/g based on consumed substrate) higher than those obtained by free cells system. Moreover, the ratio U t /U h of FFase, parameter of importance for elevated FOS production, was greater for cells immobilized in corn cobs than for free cells. Such results demonstrated that corn cobs can be successfully used as carrier for immobilization of the fungus A. japonicus , for the production of FOS and FFase.
Solange Inês Mussatto - One of the best experts on this subject based on the ideXlab platform.
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maximization of fructooligosaccharides and β fructofuranosidase production by Aspergillus japonicus under solid state fermentation conditions
Food and Bioprocess Technology, 2013Co-Authors: Solange Inês Mussatto, Lina F Ballesteros, Silvia Martins, Cristobal N Aguilar, Dulce A F Maltos, J. A. TeixeiraAbstract:The conditions of temperature, moisture content, and inoculum rate able to maximize the production of fructooligosaccharides (FOS) and β-fructofuranosidase (FFase) enzyme by solid-state fermentation were established. Fermentation assays were performed using the support material (coffee silverskin) moistened to 60, 70, or 80 % with a 240-g/l sucrose solution and inoculated with a spore suspension of Aspergillus japonicus to obtain 2 × 105, 2 × 106, or 2 × 107 spores/gram dry material. The fermentation runs were maintained under static conditions at 26, 30, or 34 °C during 20 h. The moisture content did not influence the FOS and FFase production; however, temperature between 26 and 30 °C and inoculum rate of approximately 2 × 107 spores/gram dry material maximized the results (FOS = 208.8 g/l with productivity of 10.44 g/l h; FFase = 64.12 units U/ml with productivity of 4.0 U/ml h). These results are considerably higher than those obtained under no optimized fermentation conditions and represent an important contribution for the establishment of a new industrial process for FOS and FFase production.
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process conditions that maximize the fructooligosaccharides and β fructofuranosidase production by Aspergillus japonicus under solid state fermentation conditions
XIV Congreso Nacional de Biotecnología y Bioingeniería, 2011Co-Authors: Solange Inês Mussatto, Lina F Ballesteros, Silvia Martins, Dulce A Floresmaltos, Cristobal N Aguilar, J. A. TeixeiraAbstract:Introduction. Several factors must be considered for the development of a successful bioprocess under solid-state fermentation (SSF) conditions. Some of them include the selection of the solid support, and determination of the operational conditions to be used. In a recent study, coffee silverskin (CS) was demonstrated to be a material with great potential for use as support and nutrient source for fructooligosaccharides (FOS) and β-fructofuranosidase (FFase) production by Aspergillus japonicus under SSF conditions (1).
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increase in the fructooligosaccharides yield and productivity by solid state fermentation with Aspergillus japonicus using agro industrial residues as support and nutrient source
Biochemical Engineering Journal, 2010Co-Authors: Solange Inês Mussatto, J. A. TeixeiraAbstract:Corn cobs, coffee silverskin, and cork oak were used as support and nutrient source during the fructooligosaccharides (FOS) production by Aspergillus japonicus, under solid-state fermentation (SSF) conditions. The objectives of this study consisted in evaluating the possibility of improving the FOS yield and productivity, besides to finding an alternative to reduce the production costs, and add value to these agro-industrial residues. Fermentation assays were performed by using the materials as solid support, supplemented or not with nutrients. For comparison, assays were also performed using a synthetic material as solid support, under the same operational conditions. All the material residues acted as nutrient source for the microorganism, since FOS production occurred when all of them were used without nutrient supplementation, but not when the synthetic material was used. Among the evaluated materials, coffee silverskin gave the most interesting fermentation results, with a FOS production similar in both supplemented and non-supplemented media. The elevated FOS production (128.7 g/l) and -fructofuranosidase activity (71.3 U/ml) obtained by using this material suggest SSF of coffee silverskin with A. japonicus as an interesting and promising strategy to synthesize both products at the industrial level.
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fructooligosaccharides and β fructofuranosidase production by Aspergillus japonicus immobilized on lignocellulosic materials
Journal of Molecular Catalysis B-enzymatic, 2009Co-Authors: Solange Inês Mussatto, Lígia R. Rodrigues, Cristobal N Aguilar, J. A. TeixeiraAbstract:Abstract This work describes the fructooligosaccharides (FOS) and β-fructofuranosidase (FFase) production from sucrose (200 g/l) by Aspergillus japonicus ATCC 20236 immobilized on different lignocellulosic materials including brewer's spent grain, wheat straw, corn cobs, coffee husks, cork oak, and loofa sponge. Transfructosylating (U t ) and hydrolyzing (U h ) activities of FFase were also determined. The FOS production and FFase activity ranged from 128.35 to 138.73 g/l, and 26.83 to 44.81 U/ml, respectively, for cells immobilized in the different carriers. Corn cobs was the best support material since gave the highest results of microorganism immobilization (1.49 g/g carrier), FOS and FFase production, with FOS productivity (6.61 g/l h) and yield (0.66 g/g based on total substrate; 0.73 g/g based on consumed substrate) higher than those obtained by free cells system. Moreover, the ratio U t /U h of FFase, parameter of importance for elevated FOS production, was greater for cells immobilized in corn cobs than for free cells. Such results demonstrated that corn cobs can be successfully used as carrier for immobilization of the fungus A. japonicus , for the production of FOS and FFase.
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β-Fructofuranosidase production by repeated batch fermentation with immobilized Aspergillus japonicus
Journal of Industrial Microbiology & Biotechnology, 2009Co-Authors: Solange Inês Mussatto, L. R. Rodrigues, J. A. TeixeiraAbstract:The fungus Aspergillus japonicus ATCC 20236 was immobilized in vegetal fiber and used in repeated batch fermentations of sucrose (200 g/l) for the production of β-fructofuranosidases (FFase). The assays were performed during eight consecutive cycles that were completed in a total period of 216 h. After each 24-h cycle of fermentation (except for the first cycle, which lasted 48 h), the fermented broth was replaced by fresh medium, and the FFase activity was determined in the replaced medium. The average value of FFase activity was a constant 40.6 U/ml at the end of the initial seven cycles, but had decreased by 22% at the end of the eighth cycle. Concurrent with these high and constant FFase values, the hydrolyzing activity of this enzyme increased during the cycles, while the transfructosylating activity decreased. As a consequence, the maximum production of fructooligosaccharides of 134.60 g/l observed in the initial 30 h of fermentation (first cycle) had gradually decreased by the end of the subsequent cycles, reaching approximately 23% of this value during cycles 4–8. Based on these results, we conclude that the present immobilization system has a great potential for application in a semi-continuous process for the production of FFase, but further studies are necessary to maintain the FFase transfructosylation activity at high levels during the overall process.
Isao Fujii - One of the best experts on this subject based on the ideXlab platform.
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identification of the biosynthetic gene cluster for himeic acid a a ubiquitin activating enzyme e1 inhibitor in Aspergillus japonicus mf275
ChemBioChem, 2018Co-Authors: Makoto Hashimoto, Hikaru Kato, Sachiko Tsukamoto, Ayako Katsuki, Isao FujiiAbstract:Himeic acid A, which is produced by the marine fungus Aspergillus japonicus MF275, is a specific inhibitor of the ubiquitin-activating enzyme E1 in the ubiquitin-proteasome system. To elucidate the mechanism of himeic acid biosynthesis, feeding experiments with labeled precursors have been performed. The long fatty acyl side chain attached to the pyrone ring is of polyketide origin, whereas the amide substituent is derived from leucine. These results suggest that a polyketide synthase-nonribosomal peptide synthase (PKS-NRPS) is involved in himeic acid biosynthesis. A candidate gene cluster was selected from the results of genome sequencing analysis. Disruption of the PKS-NRPS gene by Agrobacterium-mediated transformation confirms that HimA PKS-NRPS is involved in himeic acid biosynthesis. Thus, the him biosynthetic gene cluster for himeic acid in A. japonicus MF275 has been identified.
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Identification of the Biosynthetic Gene Cluster for Himeic Acid A: A Ubiquitin‐Activating Enzyme (E1) Inhibitor in Aspergillus japonicus MF275
Chembiochem : a European journal of chemical biology, 2018Co-Authors: Makoto Hashimoto, Hikaru Kato, Sachiko Tsukamoto, Ayako Katsuki, Isao FujiiAbstract:Himeic acid A, which is produced by the marine fungus Aspergillus japonicus MF275, is a specific inhibitor of the ubiquitin-activating enzyme E1 in the ubiquitin-proteasome system. To elucidate the mechanism of himeic acid biosynthesis, feeding experiments with labeled precursors have been performed. The long fatty acyl side chain attached to the pyrone ring is of polyketide origin, whereas the amide substituent is derived from leucine. These results suggest that a polyketide synthase-nonribosomal peptide synthase (PKS-NRPS) is involved in himeic acid biosynthesis. A candidate gene cluster was selected from the results of genome sequencing analysis. Disruption of the PKS-NRPS gene by Agrobacterium-mediated transformation confirms that HimA PKS-NRPS is involved in himeic acid biosynthesis. Thus, the him biosynthetic gene cluster for himeic acid in A. japonicus MF275 has been identified.
Maria De Lourdes Teixeira De Moraes Polizeli - One of the best experts on this subject based on the ideXlab platform.
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increase of the phytase production by Aspergillus japonicus and its biocatalyst potential on chicken feed treatment
Journal of Basic Microbiology, 2014Co-Authors: Alexandre Maller, Ana Claudia Vici, Tony Marcio Da Silva, Eliana Setsuko Kamimura, Maria Isabel Rodrigues, João Atílio Jorge, Hector Francisco Terenzi, Fernanda Del Antonio Facchini, Maria De Lourdes Teixeira De Moraes PolizeliAbstract:Phytase hydrolyzes phytic acid from the plant components of animal feed, releasing inorganic phosphorus. The phytase production by Aspergillus japonicus was optimized using Plackett-Burman designs (PBD), composite central rotational designs (CCRD), and response surface methodology from standard Czapek medium. The enzyme was applied in broiler chicken and laying hen foods. Analysis from PBD showed that KH2 PO2, MgSO4 · 7H2O, and yeast extract had significant influences on phytase secretion (p < 0.05). The best results from the CCRD experiments were obtained using (A) 0.040% KH2 PO4, (B) 0.050% MgSO4 · 7H2O, and (C) 0.040% yeast extract, enhancing in 49-53 U mg(-1) protein. The determination coefficient (R(2)) was 0.92 and Fcalc was 7.48 times greater than Flisted . Thus, the reduced coded model: Y (U mg-1) = 50.29 + 4.30A - 3.35(A)2 - 4.80(B)2 + 5.62C - 4.26(C)2 was considered predictive and statistically significant (p < 0.05). The optimized culture medium increased the phytase yield in 250%. A. japonicus phytase released high levels of Pi from broiler chicken and laying hen food. A. japonicus is an excellent phytase producer in a culture medium using inexpensive components and agricultural wastes. Therefore, these results provide sound arguments for the formulation of a low cost culture medium for phytase production.
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Fermentation pH in stirred tank and air-lift bioreactors affects phytase secretion by Aspergillus japonicus differently but not the particle size
Biocatalysis and Biotransformation, 2014Co-Authors: Alexandre Maller, J. A. Teixeira, André Mota, Daniel Pereira Da Silva, António A. Vicente, Maria De Lourdes Teixeira De Moraes PolizeliAbstract:AbstractPhytases are mainly produced by filamentous fungi and have great potential for biotechnological use in animal feed treatment, because this enzyme hydrolyzes ester bonds of the phytic acid releasing inositol and inorganic phosphate. The aim of this work was to evaluate the effect of pH on the production of phytase by Aspergillus japonicus in two different bioreactors, known to have different mixing patterns—stirred tank and air-lift bioreactors. The maximum phytase production—53 U/mL—was obtained at 120 h in the stirred tank while in the air-lift the maximum value was 41 U/mL, observed at 144 h. In fermentations evaluated at controlled pH values (3.5, 6.0, and 7.5), the stirred tank was more efficient for production of phytase than the air-lift. Under these conditions, the highest value was measured at 24 h and pH 3.5. These results were not closely related to fungi particle size, because hyphae with a similar diameter (0.51–0.63 mm) and sphericity (0.78–0.87 mm) secreted different amounts of phyta...
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Immobilization and high stability of an extracellular β-glucosidase from Aspergillus japonicus by ionic interactions
Journal of Molecular Catalysis B: Enzymatic, 2014Co-Authors: Tony Marcio Da Silva, João Atílio Jorge, Benevides C. C. Pessela, Jean Carlos Rodrigues Da Silva, Matheus Sanitá Lima, Jose M. Guisan, Maria De Lourdes Teixeira De Moraes PolizeliAbstract:Abstract A β-glucosidase from Aspergillus japonicus was produced on submerged fermentation using sugar cane bagasse in nature as carbon source, and incubated at 30 °C, for 72 h. After that, the dialyzed crude extract containing the active β-glucosidase was purified from three successive steps using DEAE-fractogel, MANAE-agarose and octyl-sepharose chromatographic columns. The enzyme migrated as a single band on SDS-PAGE and its molecular weight was estimated to be 114 kDa. The amino acid sequence determined by mass spectrometry demonstrated a similar structure for the β-glucosidase from Aspergillus niger and A. kawachii. The purified enzyme was immobilized by entrapment with sodium alginate beads; covalent attachment using activated CNBr-agarose and ionic interaction on MANAE-agarose and DEAE-cellulose. Soluble β-glucosidase presented a half-life of 20 min, at 60 °C, while the MANAE-agarose and DEAE-cellulose derivatives presented a half-life of 25 and 48 h, respectively. The optima pH for soluble β-glucosidase, MANAE- and DEAE derivatives was 4.5. The optimal temperature for DEAE derivative and soluble enzyme was 60 °C and for MANAE-agarose derivative was 65 °C. The Km values for soluble enzyme, MANAE-agarose and DEAE-cellulose derivatives using β-pNPG as substrate were 1.4, 2.6 and 2.0 mg/mL, respectively. Using cellobiose as substrate the Km values were 2.8, 3.4 and 3.7 mg/mL, respectively. The Vmax values using β-pNPG were 24, 25.8 and 13.5 U/mg proteins for the soluble enzyme, MANAE-agarose and DEAE-cellulose derivatives, respectively. Using cellobiose as substrate the Vmax values were 146, 152 and 100.5 U/mg protein. The MANAE and DEAE derivatives presented a good operational stability being evidenced 50 and 60% residual activity after 5 cycles of reaction with the substrate pNPβ- d -glucopyranoside.
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Increase of the phytase production by Aspergillus japonicus and its biocatalyst potential on chicken feed treatment.
Journal of basic microbiology, 2013Co-Authors: Alexandre Maller, Ana Claudia Vici, Fernanda Del Antonio Facchini, Tony Marcio Da Silva, Eliana Setsuko Kamimura, Maria Isabel Rodrigues, João Atílio Jorge, Hector Francisco Terenzi, Maria De Lourdes Teixeira De Moraes PolizeliAbstract:Phytase hydrolyzes phytic acid from the plant components of animal feed, releasing inorganic phosphorus. The phytase production by Aspergillus japonicus was optimized using Plackett-Burman designs (PBD), composite central rotational designs (CCRD), and response surface methodology from standard Czapek medium. The enzyme was applied in broiler chicken and laying hen foods. Analysis from PBD showed that KH2 PO2, MgSO4 · 7H2O, and yeast extract had significant influences on phytase secretion (p
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Optimization of fibrolytic enzyme production by Aspergillus japonicus C03 with potential application in ruminant feed and their effects on tropical forages hydrolysis
Bioprocess and biosystems engineering, 2011Co-Authors: Fernanda Dell Antonio Facchini, Ana Claudia Vici, João Atílio Jorge, Hector Francisco Terenzi, Ricardo Andrade Reis, Vivian Machado Benassi, Luiz Alexandre Pedro De Freitas, Maria De Lourdes Teixeira De Moraes PolizeliAbstract:Fibrolytic enzyme production by Aspergillus japonicus C03 was optimized in a medium containing agro-industrial wastes, supplemented with peptone and yeast extract. A 23 full factorial composite and response surface methodology were used to design the experiments and analysis of results. Tropical forages were hydrolyzed by A. japonicus C03 enzymatic extract in different levels, and they were also tested as enzymatic substrate. Optimal production to xylanase was obtained with soybean bran added to crushed corncob (1:3), 0.01% peptone, and 0.2% yeast extract, initial pH 5.0, at 30 °C under static conditions for 5 days of incubation. Optimal endoglucanase production was obtained with wheat bran added to sugarcane bagasse (3:1), 0.01% peptone, and 0.2% yeast extract, initial pH 4.0, at 30 °C, for 6 days, under static conditions. Addition of nitrogen sources as ammonium salts either inhibited or did not influence xylanase production. This enzymatic extract had a good result on tropical forage hydrolyzes and showed better performance in the Brachiaria genera, due to their low cell wall lignin quantity. These results represent a step forward toward the use of low-cost agricultural residues for the production of valuable enzymes with potential application in animal feed, using fermentation conditions.
Tony J. Fang - One of the best experts on this subject based on the ideXlab platform.
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Fermentation of rice hull by Aspergillus japonicus under ultrasonic pretreatment
Ultrasonics sonochemistry, 2011Co-Authors: Chun-yao Yang, I.-chuan Sheih, Tony J. FangAbstract:Abstract The application of ultrasound for treating rice hull used as the fermentation substrate for xylooligosaccharides production was investigated. Aspergillus japonicus CY6-1 was selected to produce cellulolytic and xylanolytic enzymes from untreated rice hull (RH) and rice hull treated with ultrasound (USRH-M). The hemicellulose yield was increased to 1.4-fold with ultrasound, and treatment time was greatly shortened from 24 h to 1.5 h at 80 °C and 300 W/28 kHz. The morphology of RH from various pretreatments was observed with field emission scanning electron microscope (FESEM), revealing the surface structure of USRH-M smoother than that of RH. USRH-M was much easier to be utilized by fungi, to extend the stability of enzyme activity and to increase activities of CMCase, β-glucosidase, and xylanase compared with those of untreated RH. The final fermentative products were xylotetraose, xylohexaose, and higher molecular weight xylooligosaccharides, achieving xylohexaose yield for USRH-M 80% higher than that for RH group.
