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Bachra Alamir - One of the best experts on this subject based on the ideXlab platform.
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Déterminations des teneurs en atractyloside dans les racines d’
EDP Sciences, 2012Co-Authors: Islam Amine Larabi, Mohamed Azzouz, Rania Abtroun, Mohamed Reggabi, Bachra AlamirAbstract:Objectif : Atractylis gummifera L., ou chardon à glu, est une plante d’Afrique du Nord appartenant à la famille des Astéracées. Elle se caractérise par la production d’un métabolite hautement toxique, appelé atractyloside (ATR). L’objectif de cette étude est d’apprécier la toxicité de cette espèce par la détermination des teneurs en atractyloside des échantillons de racine récoltés dans différentes régions d’Algérie. Méthode : Des échantillons de racine sont récoltés dans six régions différentes du pays (Alger, Tizi-Ouzou, Médéa, Béjaïa, Guelma et Tlemcen, et les extraits méthanoliques de ces derniers sont analysés par chromatographie liquide haute performance couplée à un détecteur à barrette de diode (HPLC-DAD). Résultats : La concentration en atractyloside varie de 0,1 % à 0,27 % d’atractyloside en fonction de la région avec une moyenne de 0,17 %. Discussion : Les teneurs les plus faibles sont retrouvées dans les échantillons provenant des villes côtières, et les teneurs les plus élevées proviennent de régions plus éloignées de la côte. Les quantités d’atractyloside retrouvées sont rapportées aux doses létales 50 (DL50). Le calcul montre que 260 g de racine sèche correspondent à la DL50 chez le rat par voie orale, et que pour les voies intra-péritonéale, intramusculaire, et sous-cutanée, seulement 10 à 35 g permettent d’atteindre la DL50. Chez l’Homme, il n’existe pas de données concernant les doses létales de l’atractyloside et la transposition des données animales à l’Homme ne peut être appliquée en raison de l’absence de données relatives à son volume de distribution dans l’organisme. Conclusion : L’évaluation des teneurs en atractyloside a fait l’objet de quelques études sporadiques. La plupart de ces études sont anciennes et reposent sur l’emploi de techniques d’analyse qui manquent de sensibilité et de spécificité. Ainsi, nous avons jugé utile d’initier un travail permettant d’enrichir et d’actualiser les données sur les teneurs en atractyloside du chardon à glu. Il ressort de notre étude que les teneurs en atractyloside dans la racine varient selon la région, et par conséquent, en fonction des conditions climatiques du milieu. Les chiffres obtenus présentent un appui solide pour le clinicien dans l’appréciation du degré d’imprégnation toxique lors des intoxications aiguës. Enfin, à travers ce travail, il convient d’attirer l’attention sur la nécessité d’entreprendre des travaux de recherche visant à évaluer les doses létales de l’atractyloside chez l’Homme, qui restent toujours méconnues dans la littérature scientifique
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article original original article determinations des teneurs en atractyloside dans les racines d Atractylis gummifera l provenant de six regions d algerie determinations of levels of atractyloside in the roots of Atractylis gummifera l collected from
2012Co-Authors: Islam Amine Larabi, Mohamed Azzouz, Rania Abtroun, Mohamed Reggabi, Bachra AlamirAbstract:Objective: Atractylis gummifera or glue thistle is a plant of North Africa belonging to the Asteraceae's family. It is characterized by the production of a highly toxic metabolite, called atractyloside (ATR). The purpose of this study is to assess the toxicity of this species by the determination of levels of atractyloside in root samples collected from different regions of Algeria. Method: Root samples were collected from six different regions of the country and methanol extracts were analyzed by high-performance liquid chromatography coupled with a diode array detector (HPLC-DAD). Results: The concentration of atractyloside ranges from 0.1 percent to 0.27 percent depending on the region, with an average of 0.17 percent. Discussion: The lowest levels were found in samples from coastal cities, and the highest levels from areas farther from the coast. The amounts of atractyloside found were compared with the lethal dose 50 (LD50). The results show that 260 g of dry root corresponds to the LD50 in rats orally, and for intraperitoneal, intramuscular and subcutaneous routes, only 10 to 35 g can reach the LD50. In humans, there are no data on lethal doses of atractyloside, and the transposing of animal data in humans cannot be applied because of the lack of data on the distribution volume of atractyloside. Conclusion: The assessment of levels of atractyloside has been the subject of some
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determinations des teneurs en atractyloside dans les racines d Atractylis gummifera l provenant de six regions d algerie
Annales De Toxicologie Analytique, 2012Co-Authors: Islam Amine Larabi, Mohamed Azzouz, Rania Abtroun, Mohamed Reggabi, Bachra AlamirAbstract:Objectif : Atractylis gummifera L. , ou chardon a glu, est une plante d’Afrique du Nord appartenant a la famille des Asteracees. Elle se caracterise par la production d’un metabolite hautement toxique, appele atractyloside (ATR). L’objectif de cette etude est d’apprecier la toxicite de cette espece par la determination des teneurs en atractyloside des echantillons de racine recoltes dans differentes regions d’Algerie. Methode : Des echantillons de racine sont recoltes dans six regions differentes du pays (Alger, Tizi-Ouzou, Medea, Bejaia, Guelma et Tlemcen, et les extraits methanoliques de ces derniers sont analyses par chromatographie liquide haute performance couplee a un detecteur a barrette de diode (HPLC-DAD). Resultats : La concentration en atractyloside varie de 0,1 % a 0,27 % d’atractyloside en fonction de la region avec une moyenne de 0,17 %. Discussion : Les teneurs les plus faibles sont retrouvees dans les echantillons provenant des villes cotieres, et les teneurs les plus elevees proviennent de regions plus eloignees de la cote. Les quantites d’atractyloside retrouvees sont rapportees aux doses letales 50 (DL50). Le calcul montre que 260 g de racine seche correspondent a la DL50 chez le rat par voie orale, et que pour les voies intra-peritoneale, intramusculaire, et sous-cutanee, seulement 10 a 35 g permettent d’atteindre la DL50. Chez l’Homme, il n’existe pas de donnees concernant les doses letales de l’atractyloside et la transposition des donnees animales a l’Homme ne peut etre appliquee en raison de l’absence de donnees relatives a son volume de distribution dans l’organisme. Conclusion : L’evaluation des teneurs en atractyloside a fait l’objet de quelques etudes sporadiques. La plupart de ces etudes sont anciennes et reposent sur l’emploi de techniques d’analyse qui manquent de sensibilite et de specificite. Ainsi, nous avons juge utile d’initier un travail permettant d’enrichir et d’actualiser les donnees sur les teneurs en atractyloside du chardon a glu. Il ressort de notre etude que les teneurs en atractyloside dans la racine varient selon la region, et par consequent, en fonction des conditions climatiques du milieu. Les chiffres obtenus presentent un appui solide pour le clinicien dans l’appreciation du degre d’impregnation toxique lors des intoxications aigues. Enfin, a travers ce travail, il convient d’attirer l’attention sur la necessite d’entreprendre des travaux de recherche visant a evaluer les doses letales de l’atractyloside chez l’Homme, qui restent toujours meconnues dans la litterature scientifique.
Khadija Bouabid - One of the best experts on this subject based on the ideXlab platform.
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phytochemical investigation in vitro and in vivo antioxidant properties of aqueous and organic extracts of toxic plant Atractylis gummifera l
Journal of Ethnopharmacology, 2020Co-Authors: Khadija Bouabid, Fatima Lamchouri, Hamid Toufik, My El Abbes FaouziAbstract:Abstract Ethnopharmacological relevance Atractylis gummifera is a toxic plant widely used in Mediterranean traditional medicine against colds, dizziness, and headaches, as an antisyphilitic, against boils, as a purgative, emetic and deworming. All studies reported on this plant have been carried out either on the plant and its traditional uses, or on cases of poisoning by this plant. However, few pharmacological studies have readjusted the traditional uses of this plant. Aim of the study The purpose of this article is to carry out a preliminary phytochemical study of Atractylis gummifera and to evaluate in vitro and in vivo antioxidant activity of its aqueous and organic extracts and to provide a complementary analysis of the mechanisms of action of the different antioxidant activity tests studied. Methods The phytochemical study consisted of the hot and cold preparation of aqueous extracts: (decocted, infused, macerated), organic extracts: (methanolic, methanolic macerated, chloroformic, ethyl acetate, petroleum ether) and the determination of the secondary metabolites of these extracts. In addition, the biological study consisted of evaluating antioxidant activity in vitro by five different methods (H2O2 radical reduction, DPPH, ABTS, FRAP and RP) and in vivo by SOD and MDA assays. Results The methanolic macerated is the richest in total polyphenols (102 ± 1.38 mg EAG/gE), tannins (144.09 ± 3.96 mg EC/gE) and flavonoids (17.25 ± 0.06 mg ER/gE). The same extract has the highest percentage to inhibit hydrogen peroxide (19.24 ± 1.10%) and the most potent reducing power of the ABTS radical (122.6 ± 0.63 mg ET/gE). We also noted that aqueous macerated has the most potent anti-radical activity of DPPH with an IC50 of 2.78 ± 1.03 μg/mL, the strongest reducing power of iron 96.15 ± 1.12 mg EAA/gE and which was confirmed by the FRAP test (102.5 ± 1.66 mg ET/gE). These results are in agreement with the in vivo study which showed an increase in SOD secretion in diabetic mice treated with aqueous macerated extract (904.26 ± 29.10 units/g liver and 714.16 ± 24.83 units/g kidney) and methanol macerated extract (813.61 ± 24.03 units/g liver and 719.46 ± 42.10 units/g kidney) with a statistically insignificant difference between these two extracts. Furthermore, we observed a return to normal MDA levels in mice treated with aqueous macerated extract (128.61 ± 15.76 nM/g liver and 103.18 ± 12.67 nM/g kidney) and methanol macerated extract (130.73 ± 10.73 nM/g liver and 34.28 ± 5.73 nM/g kidney). Conclusion The aqueous and organic extracts more particularly those prepared by aqueous and methanolic macerations are rich in polyphenols, flavonoids and tannins, and they represent a rich source of natural antioxidants, also they prevent lipid peroxidation and stimulate the secretion of the enzymatic antioxidant SOD
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in vivo anti diabetic effect of aqueous and methanolic macerated extracts of Atractylis gummifera
Bangladesh Journal of Pharmacology, 2019Co-Authors: Khadija Bouabid, Fatima Lamchouri, Hamid Toufik, Mohamed Boulfia, Souad Senhaji, My El Abbes FaouziAbstract:The anti-diabetic effect of Atractylis gummifera (plant used in traditional Moroccan medicine) has been evaluated in type 2 diabetic mice model. The mice were divided into five groups: Normal control, diabetic control, diabetic treated with aqueous macerate (500 mg/kg), diabetic treated with methanol macerate (500 mg/kg) and diabetic treated with metformin (300 mg/kg). The treatment of the mice was performed by daily gastric gavage for 5 weeks. The monitoring of the mice was carried out weekly by fasting glucose and measurement of biochemical parameters at the end of treatment. The aqueous macerate of A. gummifera was most effective that reduced the fasting blood glucose with 62.7%. In addition, this extract restored the biochemical parameters of diabetic mice to normal.
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In vivo anti-diabetic effect of aqueous and methanolic macerated extracts of Atractylis gummifera
'Bangladesh Journals Online (JOL)', 2019Co-Authors: Khadija Bouabid, Fatima Lamchouri, Hamid Toufik, Mohamed Boulfia, Souad Senhaji, My El Abbes FaouziAbstract:The anti-diabetic effect of Atractylis gummifera (plant used in traditional Moroccan medicine) has been evaluated in type 2 diabetic mice model. The mice were divided into five groups: Normal control, diabetic control, diabetic treated with aqueous macerate (500 mg/kg), diabetic treated with methanol macerate (500 mg/kg) and diabetic treated with metformin (300 mg/kg). The treatment of the mice was performed by daily gastric gavage for 5 weeks. The monitoring of the mice was carried out weekly by fasting glucose and measurement of biochemical parameters at the end of treatment. The aqueous macerate of A. gummifera was most effective that reduced the fasting blood glucose with 62.7%. In addition, this extract restored the biochemical parameters of diabetic mice to normal
Yvan Gaillard - One of the best experts on this subject based on the ideXlab platform.
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a validated method for quantifying atractyloside and carboxyatractyloside in blood by hplc hrms ms a non fatal case of intoxication with Atractylis gummifera l
Journal of Analytical Toxicology, 2014Co-Authors: Jeremy Carlier, Ludovic Romeuf, Jerome Guitton, Cedric Priezbarallon, Fabien Bevalot, Laurent Fanton, Yvan GaillardAbstract:: Atractyloside (ATR) and carboxyatractyloside (CATR) are diterpene glycosides that are responsible for the toxicity of several Asteraceae plants around the world. Mediterranean gum thistle (Atractylis gummifera L.) and Zulu impila (Callilepis laureola DC.), in particular, are notoriously poisonous and the cause of many accidental deaths, some suicides and even some murders. There is no current method for measuring the two toxins in biological samples that meet the criteria of specificity required in forensic medicine. We have endeavored to fill this analytical gap. Analysis was carried out using a solid-phase extraction and a high-performance liquid chromatography coupled with high-resolution tandem mass spectrometry detection. The method was validated in the whole blood with quantification limits of 0.17 and 0.15 µg/L for ATR and CATR, respectively. The method was applied to a non-fatal case of intoxication with A. gummifera. To the best of the authors' knowledge, this is the first time that a concentration of ATR and CATR in blood (883.1 and 119.0 µg/L, respectively) and urine (230.4 and 140.3 µg/L, respectively) is reported. ATR and CATR were quantified in A. gummifera roots by the standard method addition (3.7 and 5.4 mg/g, respectively).
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A Validated Method for Quantifying Atractyloside and Carboxyatractyloside in Blood by HPLC-HRMS/MS, a Non-Fatal Case of Intoxication with Atractylis gummifera L.
Journal of Analytical Toxicology, 2014Co-Authors: Jeremy Carlier, Ludovic Romeuf, Jerome Guitton, Fabien Bevalot, Laurent Fanton, Cédric Priez-barallon, Yvan GaillardAbstract:: Atractyloside (ATR) and carboxyatractyloside (CATR) are diterpene glycosides that are responsible for the toxicity of several Asteraceae plants around the world. Mediterranean gum thistle (Atractylis gummifera L.) and Zulu impila (Callilepis laureola DC.), in particular, are notoriously poisonous and the cause of many accidental deaths, some suicides and even some murders. There is no current method for measuring the two toxins in biological samples that meet the criteria of specificity required in forensic medicine. We have endeavored to fill this analytical gap. Analysis was carried out using a solid-phase extraction and a high-performance liquid chromatography coupled with high-resolution tandem mass spectrometry detection. The method was validated in the whole blood with quantification limits of 0.17 and 0.15 µg/L for ATR and CATR, respectively. The method was applied to a non-fatal case of intoxication with A. gummifera. To the best of the authors' knowledge, this is the first time that a concentration of ATR and CATR in blood (883.1 and 119.0 µg/L, respectively) and urine (230.4 and 140.3 µg/L, respectively) is reported. ATR and CATR were quantified in A. gummifera roots by the standard method addition (3.7 and 5.4 mg/g, respectively).
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o27 a validated method for quantifying atractyloside and carboxyatractyloside in blood and urine by hplc hrms ms a non fatal case of intoxication with Atractylis gummifera l
Toxicologie Analytique et Clinique, 2014Co-Authors: Jeremy Carlier, Ludovic Romeuf, Jerome Guitton, Fabien Bevalot, Laurent Fanton, Yvan GaillardAbstract:Introduction Atractyloside (ATR) and carboxyatractyloside (CATR) are diterpene glycosides that are responsible for the toxicity of several Asteraceae plants around the world. Mediterranean gum thistle ( Atractylis gummifera L.) and Zulu impila ( Callilepis laureola DC.), in particular, are notoriously poisonous and the cause of many accidental deaths, some suicides and even some murders. When a case history is not available, it is not easy to determine ATR or CATR poisoning anatomically or histologically. There is no current method for measuring the two toxins in biological samples that meet the criteria of specificity required in forensic medicine. We have endeavoured to fill this analytical gap. Methods Analysis was carried out using an original technique of high-performance liquid chromatography coupled with highresolution mass spectrometry detection (HPLC-HRMS). Given the singular structure of ATR and CATR, it is difficult to achieve and maintain the conditions required for their chromatographic separation and perfect ionization for the mass spectrometry. Separation was thus performed using an XTerra ® phenyl column (length: 150 mm; internal diameter: 2,1 mm; particle size: 3,5 μm) (Waters) [Steenkamp et al. , Forensic Sci Int, 2006, 163: 81–92] with a gradient mobile phase composed of acetonitrile containing 10% of isopropyl alcohol and a 5 mM ammonium acetate buffer at pH = 4.5. The chromatographic run time was 12.5 min. Spectrometric detection was performed using a quadrupole-Orbitrap high-resolution detector (Q Exactive™; Thermo Scientific) after ionization by heated electrospray in negative-ion mode. The mass spectrometer operated in full-scan mode and targeted-MS 2 mode alternately. MS scans (288 – 292, 723 – 727 and 767 – 771 amu) were acquired with a mass resolution of 140000. The [M. – H] − and [M – H+1] − ions of ATR (725.2154 and 726.2188 amu ± 5 ppm) and CATR (769.2053 and 770.2086 amu ± 5 ppm) were used for quantification. The fullscan product ion spectrum of the compounds (resolution of 17500) was used to confirm the identity of the toxins. The processing of the biological sample (1 mL) consisted of a protein precipitation followed by solid phase extraction on Oasis ® HLB cartridges (Waters) at pH = 4.5. Results The method was validated in the whole blood with between- and within-day RSD (relative standard deviation) less than 5.8% and 5.2% for ATR (accuracy between 95.9% and 98.6%) and 5.4% and 9.8% for CATR (accuracy between 92.0% and 107.4%). The calibration curves were linear for concentrations ranging from 0.17 to 200 μg/L for ATR and 0.15 to 200 μg/L for CATR. The detection limits were 0.066 and 0,055 μg/L respectively. ATR and CATR were quantified in blood and urine samples from a non-fatal case of intoxication by A. gummifera . The concentrations were 883.1 and 119,0 μg/L respectively in peripheral blood and 230.4 and 140,3 mg/L in urine. ATR and CATR were quantified in dried A. gummifera roots by the standard addition method. The concentrations were 3.7 and 5,4 mg/g respectively. Conclusion We present the first validated method, applicable in forensic toxicology, for quantifying ATR and CATR in whole blood. The analysis is sensitive and quick.
My El Abbes Faouzi - One of the best experts on this subject based on the ideXlab platform.
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phytochemical investigation in vitro and in vivo antioxidant properties of aqueous and organic extracts of toxic plant Atractylis gummifera l
Journal of Ethnopharmacology, 2020Co-Authors: Khadija Bouabid, Fatima Lamchouri, Hamid Toufik, My El Abbes FaouziAbstract:Abstract Ethnopharmacological relevance Atractylis gummifera is a toxic plant widely used in Mediterranean traditional medicine against colds, dizziness, and headaches, as an antisyphilitic, against boils, as a purgative, emetic and deworming. All studies reported on this plant have been carried out either on the plant and its traditional uses, or on cases of poisoning by this plant. However, few pharmacological studies have readjusted the traditional uses of this plant. Aim of the study The purpose of this article is to carry out a preliminary phytochemical study of Atractylis gummifera and to evaluate in vitro and in vivo antioxidant activity of its aqueous and organic extracts and to provide a complementary analysis of the mechanisms of action of the different antioxidant activity tests studied. Methods The phytochemical study consisted of the hot and cold preparation of aqueous extracts: (decocted, infused, macerated), organic extracts: (methanolic, methanolic macerated, chloroformic, ethyl acetate, petroleum ether) and the determination of the secondary metabolites of these extracts. In addition, the biological study consisted of evaluating antioxidant activity in vitro by five different methods (H2O2 radical reduction, DPPH, ABTS, FRAP and RP) and in vivo by SOD and MDA assays. Results The methanolic macerated is the richest in total polyphenols (102 ± 1.38 mg EAG/gE), tannins (144.09 ± 3.96 mg EC/gE) and flavonoids (17.25 ± 0.06 mg ER/gE). The same extract has the highest percentage to inhibit hydrogen peroxide (19.24 ± 1.10%) and the most potent reducing power of the ABTS radical (122.6 ± 0.63 mg ET/gE). We also noted that aqueous macerated has the most potent anti-radical activity of DPPH with an IC50 of 2.78 ± 1.03 μg/mL, the strongest reducing power of iron 96.15 ± 1.12 mg EAA/gE and which was confirmed by the FRAP test (102.5 ± 1.66 mg ET/gE). These results are in agreement with the in vivo study which showed an increase in SOD secretion in diabetic mice treated with aqueous macerated extract (904.26 ± 29.10 units/g liver and 714.16 ± 24.83 units/g kidney) and methanol macerated extract (813.61 ± 24.03 units/g liver and 719.46 ± 42.10 units/g kidney) with a statistically insignificant difference between these two extracts. Furthermore, we observed a return to normal MDA levels in mice treated with aqueous macerated extract (128.61 ± 15.76 nM/g liver and 103.18 ± 12.67 nM/g kidney) and methanol macerated extract (130.73 ± 10.73 nM/g liver and 34.28 ± 5.73 nM/g kidney). Conclusion The aqueous and organic extracts more particularly those prepared by aqueous and methanolic macerations are rich in polyphenols, flavonoids and tannins, and they represent a rich source of natural antioxidants, also they prevent lipid peroxidation and stimulate the secretion of the enzymatic antioxidant SOD
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in vivo anti diabetic effect of aqueous and methanolic macerated extracts of Atractylis gummifera
Bangladesh Journal of Pharmacology, 2019Co-Authors: Khadija Bouabid, Fatima Lamchouri, Hamid Toufik, Mohamed Boulfia, Souad Senhaji, My El Abbes FaouziAbstract:The anti-diabetic effect of Atractylis gummifera (plant used in traditional Moroccan medicine) has been evaluated in type 2 diabetic mice model. The mice were divided into five groups: Normal control, diabetic control, diabetic treated with aqueous macerate (500 mg/kg), diabetic treated with methanol macerate (500 mg/kg) and diabetic treated with metformin (300 mg/kg). The treatment of the mice was performed by daily gastric gavage for 5 weeks. The monitoring of the mice was carried out weekly by fasting glucose and measurement of biochemical parameters at the end of treatment. The aqueous macerate of A. gummifera was most effective that reduced the fasting blood glucose with 62.7%. In addition, this extract restored the biochemical parameters of diabetic mice to normal.
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In vivo anti-diabetic effect of aqueous and methanolic macerated extracts of Atractylis gummifera
'Bangladesh Journals Online (JOL)', 2019Co-Authors: Khadija Bouabid, Fatima Lamchouri, Hamid Toufik, Mohamed Boulfia, Souad Senhaji, My El Abbes FaouziAbstract:The anti-diabetic effect of Atractylis gummifera (plant used in traditional Moroccan medicine) has been evaluated in type 2 diabetic mice model. The mice were divided into five groups: Normal control, diabetic control, diabetic treated with aqueous macerate (500 mg/kg), diabetic treated with methanol macerate (500 mg/kg) and diabetic treated with metformin (300 mg/kg). The treatment of the mice was performed by daily gastric gavage for 5 weeks. The monitoring of the mice was carried out weekly by fasting glucose and measurement of biochemical parameters at the end of treatment. The aqueous macerate of A. gummifera was most effective that reduced the fasting blood glucose with 62.7%. In addition, this extract restored the biochemical parameters of diabetic mice to normal
Islam Amine Larabi - One of the best experts on this subject based on the ideXlab platform.
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Déterminations des teneurs en atractyloside dans les racines d’
EDP Sciences, 2012Co-Authors: Islam Amine Larabi, Mohamed Azzouz, Rania Abtroun, Mohamed Reggabi, Bachra AlamirAbstract:Objectif : Atractylis gummifera L., ou chardon à glu, est une plante d’Afrique du Nord appartenant à la famille des Astéracées. Elle se caractérise par la production d’un métabolite hautement toxique, appelé atractyloside (ATR). L’objectif de cette étude est d’apprécier la toxicité de cette espèce par la détermination des teneurs en atractyloside des échantillons de racine récoltés dans différentes régions d’Algérie. Méthode : Des échantillons de racine sont récoltés dans six régions différentes du pays (Alger, Tizi-Ouzou, Médéa, Béjaïa, Guelma et Tlemcen, et les extraits méthanoliques de ces derniers sont analysés par chromatographie liquide haute performance couplée à un détecteur à barrette de diode (HPLC-DAD). Résultats : La concentration en atractyloside varie de 0,1 % à 0,27 % d’atractyloside en fonction de la région avec une moyenne de 0,17 %. Discussion : Les teneurs les plus faibles sont retrouvées dans les échantillons provenant des villes côtières, et les teneurs les plus élevées proviennent de régions plus éloignées de la côte. Les quantités d’atractyloside retrouvées sont rapportées aux doses létales 50 (DL50). Le calcul montre que 260 g de racine sèche correspondent à la DL50 chez le rat par voie orale, et que pour les voies intra-péritonéale, intramusculaire, et sous-cutanée, seulement 10 à 35 g permettent d’atteindre la DL50. Chez l’Homme, il n’existe pas de données concernant les doses létales de l’atractyloside et la transposition des données animales à l’Homme ne peut être appliquée en raison de l’absence de données relatives à son volume de distribution dans l’organisme. Conclusion : L’évaluation des teneurs en atractyloside a fait l’objet de quelques études sporadiques. La plupart de ces études sont anciennes et reposent sur l’emploi de techniques d’analyse qui manquent de sensibilité et de spécificité. Ainsi, nous avons jugé utile d’initier un travail permettant d’enrichir et d’actualiser les données sur les teneurs en atractyloside du chardon à glu. Il ressort de notre étude que les teneurs en atractyloside dans la racine varient selon la région, et par conséquent, en fonction des conditions climatiques du milieu. Les chiffres obtenus présentent un appui solide pour le clinicien dans l’appréciation du degré d’imprégnation toxique lors des intoxications aiguës. Enfin, à travers ce travail, il convient d’attirer l’attention sur la nécessité d’entreprendre des travaux de recherche visant à évaluer les doses létales de l’atractyloside chez l’Homme, qui restent toujours méconnues dans la littérature scientifique
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article original original article determinations des teneurs en atractyloside dans les racines d Atractylis gummifera l provenant de six regions d algerie determinations of levels of atractyloside in the roots of Atractylis gummifera l collected from
2012Co-Authors: Islam Amine Larabi, Mohamed Azzouz, Rania Abtroun, Mohamed Reggabi, Bachra AlamirAbstract:Objective: Atractylis gummifera or glue thistle is a plant of North Africa belonging to the Asteraceae's family. It is characterized by the production of a highly toxic metabolite, called atractyloside (ATR). The purpose of this study is to assess the toxicity of this species by the determination of levels of atractyloside in root samples collected from different regions of Algeria. Method: Root samples were collected from six different regions of the country and methanol extracts were analyzed by high-performance liquid chromatography coupled with a diode array detector (HPLC-DAD). Results: The concentration of atractyloside ranges from 0.1 percent to 0.27 percent depending on the region, with an average of 0.17 percent. Discussion: The lowest levels were found in samples from coastal cities, and the highest levels from areas farther from the coast. The amounts of atractyloside found were compared with the lethal dose 50 (LD50). The results show that 260 g of dry root corresponds to the LD50 in rats orally, and for intraperitoneal, intramuscular and subcutaneous routes, only 10 to 35 g can reach the LD50. In humans, there are no data on lethal doses of atractyloside, and the transposing of animal data in humans cannot be applied because of the lack of data on the distribution volume of atractyloside. Conclusion: The assessment of levels of atractyloside has been the subject of some
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determinations des teneurs en atractyloside dans les racines d Atractylis gummifera l provenant de six regions d algerie
Annales De Toxicologie Analytique, 2012Co-Authors: Islam Amine Larabi, Mohamed Azzouz, Rania Abtroun, Mohamed Reggabi, Bachra AlamirAbstract:Objectif : Atractylis gummifera L. , ou chardon a glu, est une plante d’Afrique du Nord appartenant a la famille des Asteracees. Elle se caracterise par la production d’un metabolite hautement toxique, appele atractyloside (ATR). L’objectif de cette etude est d’apprecier la toxicite de cette espece par la determination des teneurs en atractyloside des echantillons de racine recoltes dans differentes regions d’Algerie. Methode : Des echantillons de racine sont recoltes dans six regions differentes du pays (Alger, Tizi-Ouzou, Medea, Bejaia, Guelma et Tlemcen, et les extraits methanoliques de ces derniers sont analyses par chromatographie liquide haute performance couplee a un detecteur a barrette de diode (HPLC-DAD). Resultats : La concentration en atractyloside varie de 0,1 % a 0,27 % d’atractyloside en fonction de la region avec une moyenne de 0,17 %. Discussion : Les teneurs les plus faibles sont retrouvees dans les echantillons provenant des villes cotieres, et les teneurs les plus elevees proviennent de regions plus eloignees de la cote. Les quantites d’atractyloside retrouvees sont rapportees aux doses letales 50 (DL50). Le calcul montre que 260 g de racine seche correspondent a la DL50 chez le rat par voie orale, et que pour les voies intra-peritoneale, intramusculaire, et sous-cutanee, seulement 10 a 35 g permettent d’atteindre la DL50. Chez l’Homme, il n’existe pas de donnees concernant les doses letales de l’atractyloside et la transposition des donnees animales a l’Homme ne peut etre appliquee en raison de l’absence de donnees relatives a son volume de distribution dans l’organisme. Conclusion : L’evaluation des teneurs en atractyloside a fait l’objet de quelques etudes sporadiques. La plupart de ces etudes sont anciennes et reposent sur l’emploi de techniques d’analyse qui manquent de sensibilite et de specificite. Ainsi, nous avons juge utile d’initier un travail permettant d’enrichir et d’actualiser les donnees sur les teneurs en atractyloside du chardon a glu. Il ressort de notre etude que les teneurs en atractyloside dans la racine varient selon la region, et par consequent, en fonction des conditions climatiques du milieu. Les chiffres obtenus presentent un appui solide pour le clinicien dans l’appreciation du degre d’impregnation toxique lors des intoxications aigues. Enfin, a travers ce travail, il convient d’attirer l’attention sur la necessite d’entreprendre des travaux de recherche visant a evaluer les doses letales de l’atractyloside chez l’Homme, qui restent toujours meconnues dans la litterature scientifique.