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Alice K Inouenagata - One of the best experts on this subject based on the ideXlab platform.
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survey of Begomoviruses and the crinivirus tomato chlorosis virus in solanaceous in southeast midwest of brazil
Tropical Plant Pathology, 2019Co-Authors: Tatiana Mituti, Mônika F. Moura, Mônica A. Macedo, Talita N. Z. Silva, Luiz R. Pinto, Hélcio Costa, Gabriela G. Nunes, Renate Krausesakate, Alice K Inouenagata, Mirtes F. LimaAbstract:The golden mosaic (Begomovirus) and the yellowing (crinivirus) diseases are among the main viral diseases occurring in solanaceous crops in Brazil. A survey of viruses associated with both diseases was conducted on cultivated solanaceous plants from 2013 to 2017 to study their diversity and distribution in the Southeast/Midwest regions of Brazil. Samples from potato, eggplant, sweet pepper and tomato plants were collected in fields of seven Brazilian states (Bahia, Espirito Santo, Goias, Minas Gerais, Parana, Rio de Janeiro and Sao Paulo) and in the Federal District. Total RNA/DNA was extracted and tested by RT-PCR/PCR to detect the crinivirus tomato chlorosis virus (ToCV) and Begomoviruses, respectively. Representative amplicons were directly sequenced for virus identification. Out of 343 samples, 54 were positive for ToCV: 38.6% in potato, 0.9% in sweet pepper, and 20.9% in tomato. For Begomovirus detection, 234 samples were positive. In potato and sweet pepper plants, only tomato severe rugose virus (ToSRV) was detected, while four Begomoviruses were detected in tomato plants. ToSRV was detected in 80.1% of the tomato samples, and was the predominant Begomovirus. These results indicate a low diversity of crinivirus and Begomovirus species infecting cultivated solanaceous crops in Brazil during the survey period.
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characterization of tomato leaf curl purple vein virus a new monopartite new world Begomovirus infecting tomato in northeast brazil
Archives of Virology, 2018Co-Authors: Alice K Inouenagata, Leonardo C. Albuquerque, Maria R Rojas, M A Macedo, Minor R Maliano, J O Souza, Robert L GilbertsonAbstract:A new Begomovirus species was identified from tomato plants with upward leaf curling and purple vein symptoms, which was first identified in the Piaui state of Northeast (NE) Brazil in 2014. Tomato leaf samples were collected in 2014 and 2016, and PCR with degenerate primers revealed Begomovirus infection. Rolling circle amplification and restriction enzyme digestion indicated a single genomic DNA of ~ 2.6 kb. Cloning and sequencing revealed a genome organization similar to DNA-A components of New World (NW) bipartite Begomoviruses, with no DNA-B. The complete nucleotide sequence had the highest identity (80%) with the DNA-A of Macroptilium yellow spot virus (MacYSV), and phylogenetic analyses showed it is a NW Begomovirus that clusters with MacYSV and Blainvillea yellow spot virus, also from NE Brazil. Tomato plants agroinoculated with a dimeric clone of this genomic DNA developed upward leaf curling and purple vein symptoms, indistinguishable from those observed in the field. Based on agroinoculation, this virus has a narrow host range, mainly within the family Solanaceae. Co-inoculation experiments with tomato severe rugose virus and tomato mottle leaf curl virus, the two predominant Begomoviruses infecting tomato in Brazil, revealed a synergistic interaction among these Begomoviruses. The name Tomato leaf curl purple vein virus (ToLCPVV) is proposed for this new Begomovirus.
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temporal and spatial dynamics of Begomovirus disease in tomatoes in central brazil
Plant Pathology, 2017Co-Authors: Alice K Inouenagata, Mônica Alves De ,macedo, T M Costa, J C Barbosa, J L Pereira, M Micherefffilho, R L Gilbertson, Bergamin A FilhoAbstract:Over the last two decades, Begomovirus diseases have increased in importance in Brazilian tomato crops. The major management strategy in Brazil is the application of insecticides to control the whitefly vector, but this is often unsuccessful. The objective of this work was to study the spatial and temporal progression of the disease in two processing tomato production areas in Central Brazil. A total of 24 plots (225 plants each) in six fields were evaluated weekly by visual inspection for plants with Begomovirus symptoms. The predominant Begomovirus in symptomatic tomato plants in all fields was Tomato severe rugose virus, and it was also detected in weeds and other crop plants. No correlation between incidence of Begomovirus disease and whitefly population was found. The disease progression was rapid, with a slightly aggregated distribution of symptomatic plants. No relevant differences were observed in the temporal and spatial analyses, although an important difference was detected between plots located at the centre (PC) and the edge (PE) of the fields. In the temporal analysis, the Begomovirus incidence and area under disease progress curve values were lower in PC than in PE. In the spatial analysis, plants with Begomovirus symptoms were more aggregated in PC than in PE. These results suggest that the distribution of symptomatic plants in PC and PE could be a result of three dissemination mechanisms: one random (primary) and two aggregated, a real secondary spread, and a false secondary spread. The implications of these differences on disease management are discussed. This article is protected by copyright. All rights reserved.
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molecular and biological characterization of tomato chlorotic mottle virus suggests that recombination underlies the evolution and diversity of brazilian tomato Begomoviruses
Phytopathology, 2007Co-Authors: S G Ribeiro, Darren P Martin, Cristiano Lacorte, Isabella C Simoes, Deborah R S Orlandini, Alice K InouenagataAbstract:ABSTRACT Tomato chlorotic mottle virus (ToCMoV) is an emerging Begomovirus species widely distributed throughout tomato-growing regions of Brazil. ToCMoV appears to have expanded its geographic range recently, invading tomato-growing areas that were free of Begomovirus infection before 2004. We have determined the first complete genome sequence of an infectious ToCMoV genome (isolate BA-Se1), which is the first Begomovirus species isolated in the northeast of Brazil. When introduced by particle bombardment into tomato, the cloned ToCMoV-[BA-Se1] DNA-A and DNA-B components caused typical chlorotic mottle symptoms. The cloned virus was whitefly-transmissible and, although it was infectious in hosts such as Nicotiana benthamiana, pepper, tobacco, and Nicandra physaloides, it was unable to infect Arabidopsis thaliana, bean, N. glutinosa, and Datura metel. Sequence and biological analyses indicate that ToCMoV-[BA-Se1] is a typical New World Begomovirus sp. requiring both DNA-A and DNA-B components to establish systemic infections. Although evidence of multiple recombination events was detected within the ToCMoV-[BA-Se1] DNA-A, they apparently occurred relatively long ago, implying that recombination probably has not contributed to the recent emergence of this species.
Robert L Gilbertson - One of the best experts on this subject based on the ideXlab platform.
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characterization of tomato leaf curl purple vein virus a new monopartite new world Begomovirus infecting tomato in northeast brazil
Archives of Virology, 2018Co-Authors: Alice K Inouenagata, Leonardo C. Albuquerque, Maria R Rojas, M A Macedo, Minor R Maliano, J O Souza, Robert L GilbertsonAbstract:A new Begomovirus species was identified from tomato plants with upward leaf curling and purple vein symptoms, which was first identified in the Piaui state of Northeast (NE) Brazil in 2014. Tomato leaf samples were collected in 2014 and 2016, and PCR with degenerate primers revealed Begomovirus infection. Rolling circle amplification and restriction enzyme digestion indicated a single genomic DNA of ~ 2.6 kb. Cloning and sequencing revealed a genome organization similar to DNA-A components of New World (NW) bipartite Begomoviruses, with no DNA-B. The complete nucleotide sequence had the highest identity (80%) with the DNA-A of Macroptilium yellow spot virus (MacYSV), and phylogenetic analyses showed it is a NW Begomovirus that clusters with MacYSV and Blainvillea yellow spot virus, also from NE Brazil. Tomato plants agroinoculated with a dimeric clone of this genomic DNA developed upward leaf curling and purple vein symptoms, indistinguishable from those observed in the field. Based on agroinoculation, this virus has a narrow host range, mainly within the family Solanaceae. Co-inoculation experiments with tomato severe rugose virus and tomato mottle leaf curl virus, the two predominant Begomoviruses infecting tomato in Brazil, revealed a synergistic interaction among these Begomoviruses. The name Tomato leaf curl purple vein virus (ToLCPVV) is proposed for this new Begomovirus.
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functional analysis of proteins involved in movement of the monopartite Begomovirus tomato yellow leaf curl virus
Virology, 2001Co-Authors: Maria R Rojas, Hao Jiang, Raquel Salati, Beatriz Xoconostlecazares, Mysore R Sudarshana, William J Lucas, Robert L GilbertsonAbstract:The functional properties of proteins [capsid protein (CP), V1, and C4] potentially involved with movement of the monopartite Begomovirus, Tomato yellow leaf curl virus (TYLCV), were investigated using microinjection of Escherichia coli expressed proteins and transient expression of GFP fusion proteins. The TYLCV CP localized to the nucleus and nucleolus and acted as a nuclear shuttle, facilitating import and export of DNA. Thus, the CP serves as the functional homolog of the bipartite Begomovirus BV1. The TYLCV V1 localized around the nucleus and at the cell periphery and colocalized with the endoplasmic reticulum, whereas C4 was localized to the cell periphery. Together, these patterns of localization were similar to that of the bipartite Begomovirus BC1, known to mediate cell-to-cell movement. However, in contrast to BC1, V1 and C4, alone or in combination, had a limited capacity to move and mediate macromolecular trafficking through mesophyll or epidermal plasmodesmata. Immunolocalization and in situ PCR experiments, conducted with tomato plants at three stages of development, established that TYLCV infection was limited to phloem cells of shoot apical, leaf, stem, and floral tissues. Thus, the V1 and/or C4 may be analogs of the bipartite Begomovirus BC1 that have evolved to mediate TYLCV movement within phloem tissue.
M A Macedo - One of the best experts on this subject based on the ideXlab platform.
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characterization of tomato leaf curl purple vein virus a new monopartite new world Begomovirus infecting tomato in northeast brazil
Archives of Virology, 2018Co-Authors: Alice K Inouenagata, Leonardo C. Albuquerque, Maria R Rojas, M A Macedo, Minor R Maliano, J O Souza, Robert L GilbertsonAbstract:A new Begomovirus species was identified from tomato plants with upward leaf curling and purple vein symptoms, which was first identified in the Piaui state of Northeast (NE) Brazil in 2014. Tomato leaf samples were collected in 2014 and 2016, and PCR with degenerate primers revealed Begomovirus infection. Rolling circle amplification and restriction enzyme digestion indicated a single genomic DNA of ~ 2.6 kb. Cloning and sequencing revealed a genome organization similar to DNA-A components of New World (NW) bipartite Begomoviruses, with no DNA-B. The complete nucleotide sequence had the highest identity (80%) with the DNA-A of Macroptilium yellow spot virus (MacYSV), and phylogenetic analyses showed it is a NW Begomovirus that clusters with MacYSV and Blainvillea yellow spot virus, also from NE Brazil. Tomato plants agroinoculated with a dimeric clone of this genomic DNA developed upward leaf curling and purple vein symptoms, indistinguishable from those observed in the field. Based on agroinoculation, this virus has a narrow host range, mainly within the family Solanaceae. Co-inoculation experiments with tomato severe rugose virus and tomato mottle leaf curl virus, the two predominant Begomoviruses infecting tomato in Brazil, revealed a synergistic interaction among these Begomoviruses. The name Tomato leaf curl purple vein virus (ToLCPVV) is proposed for this new Begomovirus.
Shahid Mansoor - One of the best experts on this subject based on the ideXlab platform.
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Association of cotton leaf curl Multan betasatellite and Ageratum conyzoides symptomless alphasatellite with tomato leaf curl New Delhi virus in Luffa cylindrica in Pakistan
Australasian Plant Pathology, 2020Co-Authors: Ifrah Anwar, Imran Amin, Hanan Arif Bukhari, Nazia Nahid, Kamran Rashid, Shabnum Shaheen, Khadim Hussain, Shahid MansoorAbstract:Luffa plants showing symptoms of Begomovirus infection were collected from Vehari, Punjab in 2016. RCA and PCR amplifications of Begomovirus genomic components showed the presence of a typical Old World bipartite Begomovirus Tomato leaf curl New Delhi virus (ToLCNDV). Interestingly, Ageratum conyzoides symptomless alphasatellite and CLCuMB were also found associated with bipartite Begomoviruses in the same host, however, no monopartite Begomovirus was detected. To the best of our knowledge, this is the first report of ToLCNDV with associated satellites infecting L. cylindrica in Pakistan. The importance of Begomovirus extended host range with different combinations of associated satellites has been discussed.
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INTERNATIONAL JOURNAL OF AGRICULTURE & BIOLOGY Chili Leaf Curl Betasatellite Enhances Symptoms Induced by Tomato Leaf Curl New Delhi Virus, a Bipartite Begomovirus
2020Co-Authors: Adnan Akhter, Sohail Akhtar, Muhammad Saeed, Shahid MansoorAbstract:Abstract Begomovirus disease complexes are the major limiting factor on chilies throughout the Indian subcontinent. The severe symptoms in chilies are associated with multiple Begomovirus components. Infectivity assays of Tomato leaf curl New Delhi virus (ToLCNDV) and Chili leaf curl betasatellite (ChLCB) were conducted. DNA A and DNA B of ToLCNDV isolated from chilies and tomato were infectious and produced leaf curl symptoms when inoculated on Nicotiana benthamiana by biolistic gun method. Co-inoculation of ToLCNDV with ChLCB resulted in the severity of disease symptoms. These results show that interaction of betasatellite with bipartite Begomoviruses may enhance symptoms induced by bipartite viruses. Thus interactions of betasatellites and symptom enhancement are not limited to monopartite Begomoviruses and bipartite Begomovirus-betasatellite interaction presents yet another example of rapid changes in Begomovirus complexes that infect important crops in the region
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Tomato leaf curl New Delhi virus: a widespread bipartite Begomovirus in the territory of monopartite Begomoviruses.
Molecular plant pathology, 2016Co-Authors: Syed Shan-e-ali Zaidi, Darren P Martin, Imran Amin, Muhammad Farooq, Shahid MansoorAbstract:ummary Tomato leaf curl New Delhi virus (ToLCNDV) is an exceptional Old World bipartite Begomovirus. On the Indian subcontinent, a region in which monopartite DNA satellite-associated Begomoviruses with mostly narrow geographical ranges predominate, it is widespread, with a geographical range also including the Far East, Middle East, North Africa and Europe. The success of ToLCNDV probably derives from its broad host range and highly flexible genomic configuration: its DNA-A component is capable of productively interacting with, and trans-replicating, diverse DNA-B components and betasatellites. An understanding of the capacity of ToLCNDV to infect a variety of hosts and spread across a broad and ecologically variable geographical range could illuminate the potential economic threats associated with similar begomoviral invasions. Towards this end, we used available ToLCNDV sequences to reconstruct the history of ToLCNDV spread. Taxonomy Family Geminiviridae, Genus Begomovirus. ToLCNDV is a bipartite Begomovirus. Following the revised Begomovirus taxonomic criteria of 91% and 94% nucleotide identity for species and strain demarcation, respectively, ToLCNDV is a distinct species with two strains: ToLCNDV and ToLCNDV-Spain. Host range The primary cultivated host of ToLCNDV is tomato (Solanum lycopersicum), but the virus is also known to infect 43 other plant species from a range of families, including Cucurbitaceae, Euphorbiaceae, Solanaceae, Malvaceae and Fabaceae. Disease symptoms Typical symptoms of ToLCNDV infection in its various hosts include leaf curling, vein thickening, puckering, purpling/darkening of leaf margins, leaf area reduction, internode shortening and severe stunting.
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xanthium strumarium a weed host of components of Begomovirus betasatellite complexes affecting crops
Virus Genes, 2012Co-Authors: M Mubin, Rob W. Briddon, Imran Amin, Sohail Akhtar, Shahid MansoorAbstract:Xanthium strumarium is a common weed that often shows symptoms typical of Begomovirus infection, such as leaf curling and vein thickening. The virus complex isolated from the weed consisted of two Begomoviruses along with a betasatellite and an alphasatellite. The first Begomovirus was shown to be an isolate of Cotton leaf curl Burewala virus, a new recombinant Begomovirus species that is associated with resistance breaking in previously resistant cotton varieties in Pakistan, whereas the second was shown to be an isolate of Tomato leaf curl Gujarat virus (ToLCGV), a Begomovirus previously reported to be bipartite. However, there was no evidence for the presence of the second genomic component, DNA B, of ToLCGV in X. strumarium. The betasatellite was shown to be an isolate of Tomato yellow leaf curl Thailand betasatellite, the first time this satellite has been identified in Pakistan. The alphasatellite associated with infection of X. strumarium was shown to be a species recently identified in potato and various weeds; Potato leaf curl alphasatellite. Although each component has been identified previously, this is the first time they have been identified in a single host. These findings reinforce the hypothesis that weeds are reservoirs of crop-infecting Begomoviruses that may contribute to virus diversity by virtue of harboring multiple viruses and virus associated components, which may lead to interspecific recombination and component exchange.
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Post-transcriptional gene silencing suppressor activity of two non-pathogenic alphasatellites associated with a Begomovirus
Virology, 2010Co-Authors: Muhammad Shah Nawaz-ul-rehman, Shahid Mansoor, Nazia Nahid, Rob W. Briddon, Claude M. FauquetAbstract:Abstract Alphasatellites and betasatellites are Begomovirus-associated single-stranded circular DNA molecules. Two distinct alphasatellites, Gossypium darwinii symptomless alphasatellite and Gossypium mustelinium symptomless alphasatellite, were previously isolated from Gossypium davidsonii and G. mustelinium . Here we show that the replication-associated proteins (Rep: a rolling-circle replication initiator protein) encoded by these alphasatellites interact with the Rep and C4 proteins encoded by their helper Begomovirus, Cotton leaf curl Rajasthan virus (CLCuRaV), in a yeast two-hybrid assay. Both the alphasatellite-encoded Reps were found to have strong gene silencing suppressor activity, in contrast to the betasatellite-encoded βC1 and CLCuRaV-encoded C2, C4 and V2 proteins. The presence of alphasatellites maintained suppression of gene silencing in the youngest, actively growing tissue of CLCuRaV-betasatellite-infected plants. This is the first demonstration of a rolling-circle replication initiator protein with suppressor of gene silencing activity and provides a possible explanation for the selective advantage provided by the association of alphasatellites with Begomovirus–betasatellite complexes.
Leonardo C. Albuquerque - One of the best experts on this subject based on the ideXlab platform.
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characterization of tomato leaf curl purple vein virus a new monopartite new world Begomovirus infecting tomato in northeast brazil
Archives of Virology, 2018Co-Authors: Alice K Inouenagata, Leonardo C. Albuquerque, Maria R Rojas, M A Macedo, Minor R Maliano, J O Souza, Robert L GilbertsonAbstract:A new Begomovirus species was identified from tomato plants with upward leaf curling and purple vein symptoms, which was first identified in the Piaui state of Northeast (NE) Brazil in 2014. Tomato leaf samples were collected in 2014 and 2016, and PCR with degenerate primers revealed Begomovirus infection. Rolling circle amplification and restriction enzyme digestion indicated a single genomic DNA of ~ 2.6 kb. Cloning and sequencing revealed a genome organization similar to DNA-A components of New World (NW) bipartite Begomoviruses, with no DNA-B. The complete nucleotide sequence had the highest identity (80%) with the DNA-A of Macroptilium yellow spot virus (MacYSV), and phylogenetic analyses showed it is a NW Begomovirus that clusters with MacYSV and Blainvillea yellow spot virus, also from NE Brazil. Tomato plants agroinoculated with a dimeric clone of this genomic DNA developed upward leaf curling and purple vein symptoms, indistinguishable from those observed in the field. Based on agroinoculation, this virus has a narrow host range, mainly within the family Solanaceae. Co-inoculation experiments with tomato severe rugose virus and tomato mottle leaf curl virus, the two predominant Begomoviruses infecting tomato in Brazil, revealed a synergistic interaction among these Begomoviruses. The name Tomato leaf curl purple vein virus (ToLCPVV) is proposed for this new Begomovirus.
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A novel monopartite Begomovirus infecting sweet potato in Brazil.
Archives of virology, 2011Co-Authors: Leonardo C. Albuquerque, Enrique Moriones, Alice K. Inoue-nagata, Bruna Pinheiro, Simone G. Ribeiro, Renato O. Resende, Jesús Navas-castilloAbstract:The complete genome sequences of two monopartite Begomovirus isolates (genus Begomovirus, family Geminiviridae) present in a single sweet potato (Ipomoea batatas) plant collected in Sao Paulo, Brazil, are presented. Based on the current taxonomic criteria for the genus Begomovirus, one of the isolates was shown to represent a novel species, tentatively named Sweet potato leaf curl Sao Paulo virus (SPLCSPV). The other isolate represented a new strain of sweet potato leaf curl virus, named sweet potato leaf curl virus-Sao Paulo (SPLCV-SP). The full genome sequence of the SPLCSPV isolate shared the highest nucleotide identity (87.6%) with isolates of sweet potato leaf curl Spain virus (SPLCESV). Phylogenetic and recombination analyses were used to investigate the relationships of these isolates to other monopartite Ipomoea-infecting Begomoviruses.