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Ning-fu Peng - One of the best experts on this subject based on the ideXlab platform.
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clinicopathological characteristics and liver stem cell marker expression in hepatocellular carcinoma involving Bile Duct Tumor thrombi
Tumor Biology, 2016Co-Authors: Ye-bin Pang, Jian-hong Zhong, Xiao-ling Luo, Zhe Guo, Bang-de Xiang, Ning-fu PengAbstract:The aim of this study was to analyze the clinicopathological characteristics and expression of liver stem cell markers of hepatocellular carcinoma (HCC) involving Bile Duct Tumor thrombi (BDTT). A total of 35 patients with HCC and BDTT in a consecutive series of HCC patients who underwent surgical treatment were studied retrospectively and compared with 916 patients without BDTT from the same series. Clinicopathological characteristics, overall survival (OS), and Tumor expression of liver stem cell markers CD133, CD90, EpCAM, CK19, VEGF, and C-kit were compared between the two patient groups. Analysis was performed for the entire patient groups as well as for 35 pairs of patients with or without BDTT matched by propensity score. HCC patients with BDTT tended to have smaller Tumors than those without BDTT, as well as a higher probability of having poorly differentiated Tumor, Child-Pugh class B, liver cirrhosis, and microvascular invasion. Tumor tissue in patients with BDTT showed significantly higher expression rates of all liver stem cell markers examined. OS was significantly lower for patients with BDTT at 1 year (69 vs 84 %), 3 years (37 vs 64 %), and 5 years (20 vs 55 %) (P < 0.001). Patients with HCC and BDTT show lower OS than patients without BDTT. The higher frequency of liver stem cell marker expression in the presence of BDTT suggests that such stem cells may play a role in the pathogenesis of this form of HCC.
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Clinicopathological characteristics and liver stem cell marker expression in hepatocellular carcinoma involving Bile Duct Tumor thrombi.
Tumor Biology, 2015Co-Authors: Ye-bin Pang, Jian-hong Zhong, Xiao-ling Luo, Zhe Guo, Bang-de Xiang, Ning-fu PengAbstract:The aim of this study was to analyze the clinicopathological characteristics and expression of liver stem cell markers of hepatocellular carcinoma (HCC) involving Bile Duct Tumor thrombi (BDTT). A total of 35 patients with HCC and BDTT in a consecutive series of HCC patients who underwent surgical treatment were studied retrospectively and compared with 916 patients without BDTT from the same series. Clinicopathological characteristics, overall survival (OS), and Tumor expression of liver stem cell markers CD133, CD90, EpCAM, CK19, VEGF, and C-kit were compared between the two patient groups. Analysis was performed for the entire patient groups as well as for 35 pairs of patients with or without BDTT matched by propensity score. HCC patients with BDTT tended to have smaller Tumors than those without BDTT, as well as a higher probability of having poorly differentiated Tumor, Child-Pugh class B, liver cirrhosis, and microvascular invasion. Tumor tissue in patients with BDTT showed significantly higher expression rates of all liver stem cell markers examined. OS was significantly lower for patients with BDTT at 1 year (69 vs 84 %), 3 years (37 vs 64 %), and 5 years (20 vs 55 %) (P
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Liver Stem/Progenitor Cells in the Canals of Hering: Cellular Origin of Hepatocellular Carcinoma with Bile Duct Tumor Thrombi?
Stem Cell Reviews and Reports, 2010Co-Authors: Ning-fu Peng, Xiang Cai, Shaozao TanAbstract:It is generally believed that the invasion of hepatocellular carcinoma (HCC) into the biliary tree ultimately leads to the formation of Bile Duct Tumor thrombi (BDTT). However, recent studies revealed that primary Tumor might be small, even undetectable, and there was no histopathologic evidence of direct Tumor invasion into Bile Duct wall in some patients. During the last decade, efforts on stem cell biology may shed light on the pathogenesis of BDTT. Presently, accumulating evidence supports the following notions: (1) the canals of Hering (CoH) are the most likely origin of liver stem/progenitor cells (LSPCs) in adult livers; (2) similar signalling pathways may regulate self-renewal in LSPCs and liver cancer cells, and a substantial proportion of liver Tumors may often originate from the transformation of LSPCs; and (3) liver cancer contains rare cells with stem cell-like properties, which could derive from malignant transformation of LSPCs. Herein, we propose that HCC with BDTT, especially with small or undetectable primary lesion and/or no histopathologic evidence for Bile Duct invasion, might arise from LSPCs residing in the CoH and, possibly, some primary lesions are formed firstly within the intrahepatic biliary tree. When “Tumor thrombi” extends mainly along Bile Duct, there might be “BDTT” alone; when it invades into surrounding parenchyma, there might often be small “primary Tumor” with “BDTT”. If this holds true, the putative type may be a particular subset of HCC, and most importantly it would facilitate our understanding of stem-cell origin of HCC.
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liver stem progenitor cells in the canals of hering cellular origin of hepatocellular carcinoma with Bile Duct Tumor thrombi
Stem Cell Reviews and Reports, 2010Co-Authors: Ning-fu Peng, Xiang Cai, Shaozao TanAbstract:It is generally believed that the invasion of hepatocellular carcinoma (HCC) into the biliary tree ultimately leads to the formation of Bile Duct Tumor thrombi (BDTT). However, recent studies revealed that primary Tumor might be small, even undetectable, and there was no histopathologic evidence of direct Tumor invasion into Bile Duct wall in some patients. During the last decade, efforts on stem cell biology may shed light on the pathogenesis of BDTT. Presently, accumulating evidence supports the following notions: (1) the canals of Hering (CoH) are the most likely origin of liver stem/progenitor cells (LSPCs) in adult livers; (2) similar signalling pathways may regulate self-renewal in LSPCs and liver cancer cells, and a substantial proportion of liver Tumors may often originate from the transformation of LSPCs; and (3) liver cancer contains rare cells with stem cell-like properties, which could derive from malignant transformation of LSPCs. Herein, we propose that HCC with BDTT, especially with small or undetectable primary lesion and/or no histopathologic evidence for Bile Duct invasion, might arise from LSPCs residing in the CoH and, possibly, some primary lesions are formed firstly within the intrahepatic biliary tree. When “Tumor thrombi” extends mainly along Bile Duct, there might be “BDTT” alone; when it invades into surrounding parenchyma, there might often be small “primary Tumor” with “BDTT”. If this holds true, the putative type may be a particular subset of HCC, and most importantly it would facilitate our understanding of stem-cell origin of HCC.
Shi-ting Feng - One of the best experts on this subject based on the ideXlab platform.
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Hepatocellular carcinoma with hilar Bile Duct Tumor thrombus versus hilar Cholangiocarcinoma on enhanced computed tomography: a diagnostic challenge.
BMC Cancer, 2020Co-Authors: Xiaoqi Zhou, Jifei Wang, Mimi Tang, Mengqi Huang, Zhenpeng Peng, Shi-ting FengAbstract:Hepatocellular carcinoma (HCC) with hilar Bile Duct Tumor thrombus (HBDTT) often mimic hilar cholangiocarcinoma (hilar CC). The purpose of this study is to analyze the Computed Tomography (CT) characteristics of HCC with HBDTT and to identify imaging features to aid its differentiation from hilar CC on enhanced CT. We retrospectively identified 58 cases with pathologically proved HCC with HBDTT between 2011 and 2018. Seventy-seven cases of pathologically proven hilar CCs were selected during the same period. The clinical features and CT findings of the two groups were reviewed and compared. HCC with HBDTTs are more commonly found in men (87.9% vs 63.6%, p = 0.001) with lower age of onset (49.84 vs 58.61 years; p
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hepatocellular carcinoma with hilar Bile Duct Tumor thrombus versus hilar cholangiocarcinoma on enhanced computed tomography a diagnostic challenge
BMC Cancer, 2020Co-Authors: Xiaoqi Zhou, Jifei Wang, Mimi Tang, Mengqi Huang, Zhenpeng Peng, Shi-ting FengAbstract:Hepatocellular carcinoma (HCC) with hilar Bile Duct Tumor thrombus (HBDTT) often mimic hilar cholangiocarcinoma (hilar CC). The purpose of this study is to analyze the Computed Tomography (CT) characteristics of HCC with HBDTT and to identify imaging features to aid its differentiation from hilar CC on enhanced CT. We retrospectively identified 58 cases with pathologically proved HCC with HBDTT between 2011 and 2018. Seventy-seven cases of pathologically proven hilar CCs were selected during the same period. The clinical features and CT findings of the two groups were reviewed and compared. HCC with HBDTTs are more commonly found in men (87.9% vs 63.6%, p = 0.001) with lower age of onset (49.84 vs 58.61 years; p < 0.001) in comparison to hilar CCs. Positive correlation were identified between HCC with HBDTTs and chronic HBV infection (72.4% vs 11.7%; p < 0.001), increased serum AFP (67.2% vs 1.3%; p < 0.001), CA19–9 level (58.6% vs 85.7%; p < 0.001) and CEA level (3.4% vs 29.9%; p = 0.001), parenchymal lesion with intraDuctal lesion (100% vs 18.2%; p < 0.001), washout during the portal venous phase (84.5% vs 6.5%; p < 0.001), thickened Bile Duct wall (8.6% vs 93.5%; p < 0.001), intrahepatic vascular embolus (44.8% vs 7.8%; p < 0.001), splenomegaly (34.5% vs 2.6%, p < 0.001). A scoring system consisting of the five parameters obtained from characteristics mentioned above was trialed. The sensitivity and specificity for diagnosing HCC with HBDTT were 96.39, 100 and 92.5% respectively when the total score was 2 or more. HCC with HBDTTs are often distinguishable from hilar CCs based on washout during portal venous phase without thickened Bile Duct wall. HBV infection and serum AFP level facilitate the differentiation.
Xiaoqi Zhou - One of the best experts on this subject based on the ideXlab platform.
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hepatocellular carcinoma with hilar Bile Duct Tumor thrombus versus hilar cholangiocarcinoma on enhanced computed tomography a diagnostic challenge
BMC Cancer, 2020Co-Authors: Xiaoqi Zhou, Jifei Wang, Mimi Tang, Mengqi Huang, Zhenpeng Peng, Shi-ting FengAbstract:Hepatocellular carcinoma (HCC) with hilar Bile Duct Tumor thrombus (HBDTT) often mimic hilar cholangiocarcinoma (hilar CC). The purpose of this study is to analyze the Computed Tomography (CT) characteristics of HCC with HBDTT and to identify imaging features to aid its differentiation from hilar CC on enhanced CT. We retrospectively identified 58 cases with pathologically proved HCC with HBDTT between 2011 and 2018. Seventy-seven cases of pathologically proven hilar CCs were selected during the same period. The clinical features and CT findings of the two groups were reviewed and compared. HCC with HBDTTs are more commonly found in men (87.9% vs 63.6%, p = 0.001) with lower age of onset (49.84 vs 58.61 years; p < 0.001) in comparison to hilar CCs. Positive correlation were identified between HCC with HBDTTs and chronic HBV infection (72.4% vs 11.7%; p < 0.001), increased serum AFP (67.2% vs 1.3%; p < 0.001), CA19–9 level (58.6% vs 85.7%; p < 0.001) and CEA level (3.4% vs 29.9%; p = 0.001), parenchymal lesion with intraDuctal lesion (100% vs 18.2%; p < 0.001), washout during the portal venous phase (84.5% vs 6.5%; p < 0.001), thickened Bile Duct wall (8.6% vs 93.5%; p < 0.001), intrahepatic vascular embolus (44.8% vs 7.8%; p < 0.001), splenomegaly (34.5% vs 2.6%, p < 0.001). A scoring system consisting of the five parameters obtained from characteristics mentioned above was trialed. The sensitivity and specificity for diagnosing HCC with HBDTT were 96.39, 100 and 92.5% respectively when the total score was 2 or more. HCC with HBDTTs are often distinguishable from hilar CCs based on washout during portal venous phase without thickened Bile Duct wall. HBV infection and serum AFP level facilitate the differentiation.
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Hepatocellular carcinoma with hilar Bile Duct Tumor thrombus versus hilar Cholangiocarcinoma on enhanced computed tomography: a diagnostic challenge.
BMC Cancer, 2020Co-Authors: Xiaoqi Zhou, Jifei Wang, Mimi Tang, Mengqi Huang, Zhenpeng Peng, Shi-ting FengAbstract:Hepatocellular carcinoma (HCC) with hilar Bile Duct Tumor thrombus (HBDTT) often mimic hilar cholangiocarcinoma (hilar CC). The purpose of this study is to analyze the Computed Tomography (CT) characteristics of HCC with HBDTT and to identify imaging features to aid its differentiation from hilar CC on enhanced CT. We retrospectively identified 58 cases with pathologically proved HCC with HBDTT between 2011 and 2018. Seventy-seven cases of pathologically proven hilar CCs were selected during the same period. The clinical features and CT findings of the two groups were reviewed and compared. HCC with HBDTTs are more commonly found in men (87.9% vs 63.6%, p = 0.001) with lower age of onset (49.84 vs 58.61 years; p
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CT findings and clinical application of hepatocellular carcinoma with Bile Duct Tumor thrombus
2019Co-Authors: Weiqiang Liang, Huasong Cai, Xiaoqi Zhou, Meng WangAbstract:Objective To analyze the CT findings of hepatocellular carcinoma (HCC) with Bile Duct Tumor thrombus (BDTT). Methods From January 2012 to September 2018, twenty-six cases of HCC with BDTT were collected from the First Affiliated Hospital of Sun Yat-sen University and Xinhui Traditional Chinese Medicine Hospital. All cases were confirmed by pathology after operation, 64-slice spiral CT plain scan and dual-phase enhanced scan (arterial phase and portal phase) were performed. The imaging features, including distribution, density and enhancement patterns of HCC and BDTT, relationship of HCC with BDTT and situation of the Bile Duct wall, were retrospectively analyzed. Based on the results of operation and pathology as "golden standard", the accuracy of CT in localization and qualitative analysis of HCC with BDTT was analyzed. Results All the 26 cases were diagnosed as intrahepatic and intraDuctal masses, HCC and BDTT clearly displayed on CT. On contrast-enhanced CT images, HCC with BDTT showed three types of enhancement pattern: fast in and fast out (18 cases, 69.2%), fast in and slow out (4 cases, 15.4%), and all low density (4 cases, 15.4%). BDTT showed dilated Bile Ducts filled and soft tissue masses, with distal Bile Duct dilation, without Bile Duct wall thickening. HCC was directly associated with BDTT in 24 cases (92.3%), but not associated in 2 cases (7.7%). The enhancement type of BDTT was similar with HCC lesion in 25 cases (96.2%), but different in 1 case (3.8%). The accuracy of CT in detecting HCC with BDTT was 100.0% (26/26). Conclusion The CT imaging of HCC with BDTT has certain characteristics, which is helpful for the diagnosis of the disease. Key words: Carcinoma, hepatocellular; Neoplastic cells, circulating; Tomography, spiral computed; Bile Duct Tumor thrombus
Shaozao Tan - One of the best experts on this subject based on the ideXlab platform.
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Liver Stem/Progenitor Cells in the Canals of Hering: Cellular Origin of Hepatocellular Carcinoma with Bile Duct Tumor Thrombi?
Stem Cell Reviews and Reports, 2010Co-Authors: Ning-fu Peng, Xiang Cai, Shaozao TanAbstract:It is generally believed that the invasion of hepatocellular carcinoma (HCC) into the biliary tree ultimately leads to the formation of Bile Duct Tumor thrombi (BDTT). However, recent studies revealed that primary Tumor might be small, even undetectable, and there was no histopathologic evidence of direct Tumor invasion into Bile Duct wall in some patients. During the last decade, efforts on stem cell biology may shed light on the pathogenesis of BDTT. Presently, accumulating evidence supports the following notions: (1) the canals of Hering (CoH) are the most likely origin of liver stem/progenitor cells (LSPCs) in adult livers; (2) similar signalling pathways may regulate self-renewal in LSPCs and liver cancer cells, and a substantial proportion of liver Tumors may often originate from the transformation of LSPCs; and (3) liver cancer contains rare cells with stem cell-like properties, which could derive from malignant transformation of LSPCs. Herein, we propose that HCC with BDTT, especially with small or undetectable primary lesion and/or no histopathologic evidence for Bile Duct invasion, might arise from LSPCs residing in the CoH and, possibly, some primary lesions are formed firstly within the intrahepatic biliary tree. When “Tumor thrombi” extends mainly along Bile Duct, there might be “BDTT” alone; when it invades into surrounding parenchyma, there might often be small “primary Tumor” with “BDTT”. If this holds true, the putative type may be a particular subset of HCC, and most importantly it would facilitate our understanding of stem-cell origin of HCC.
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liver stem progenitor cells in the canals of hering cellular origin of hepatocellular carcinoma with Bile Duct Tumor thrombi
Stem Cell Reviews and Reports, 2010Co-Authors: Ning-fu Peng, Xiang Cai, Shaozao TanAbstract:It is generally believed that the invasion of hepatocellular carcinoma (HCC) into the biliary tree ultimately leads to the formation of Bile Duct Tumor thrombi (BDTT). However, recent studies revealed that primary Tumor might be small, even undetectable, and there was no histopathologic evidence of direct Tumor invasion into Bile Duct wall in some patients. During the last decade, efforts on stem cell biology may shed light on the pathogenesis of BDTT. Presently, accumulating evidence supports the following notions: (1) the canals of Hering (CoH) are the most likely origin of liver stem/progenitor cells (LSPCs) in adult livers; (2) similar signalling pathways may regulate self-renewal in LSPCs and liver cancer cells, and a substantial proportion of liver Tumors may often originate from the transformation of LSPCs; and (3) liver cancer contains rare cells with stem cell-like properties, which could derive from malignant transformation of LSPCs. Herein, we propose that HCC with BDTT, especially with small or undetectable primary lesion and/or no histopathologic evidence for Bile Duct invasion, might arise from LSPCs residing in the CoH and, possibly, some primary lesions are formed firstly within the intrahepatic biliary tree. When “Tumor thrombi” extends mainly along Bile Duct, there might be “BDTT” alone; when it invades into surrounding parenchyma, there might often be small “primary Tumor” with “BDTT”. If this holds true, the putative type may be a particular subset of HCC, and most importantly it would facilitate our understanding of stem-cell origin of HCC.
Hong Zeng - One of the best experts on this subject based on the ideXlab platform.
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dysregulation of bmi1 promotes malignant transformation of hepatic progenitor cells
Oncogenesis, 2016Co-Authors: Rui Zhang, Leibo Xu, Wenrui Wu, Hong ZengAbstract:Adult hepatic progenitor cells (HPCs) are involved in a wide range of human liver diseases, including hepatocellular carcinoma (HCC). Bmi1 has been reported to have vital roles in stem cell self-renewal and carcinogenesis. We have previously demonstrated that Bmi1 is upregulated in HCC with Bile Duct Tumor thrombi, a subtype of HCC characterized by profuse expression of hepatic stem cell markers. However, the function of Bmi1 in HPCs has not yet been well elucidated. The current study was designed to investigate the effects of Bmi1 on the biological properties of rat HPCs. To accomplish this, Bmi1 was silenced or enhanced in two HPC cell lines (WB-F344 and OC3) by, respectively, using either small interfering RNA against Bmi1 or a forced Bmi1 expression retroviral vector. The biological functions of Bmi1 in HPCs were investigated through cell proliferation assays, colony-formation assays, cell cycle analysis and invasion assays, as well as through xenograft-formation assays. In this study, genetic depletion of Bmi1 repressed cell proliferation, colony formation and invasion in both assessed HPC cell lines relative to controls. Conversely, forced expression of Bmi1 in two HPCs cell lines promoted cell proliferation, colony formation and invasion in vitro. Aldehyde dehydrogenase (ALDH) assay revealed a significant increase in the number of ALDH-positive cells following the forced expression of Bmi1 in HPCs. Most importantly, transplantation of forced Bmi1 expression HPCs into nude mice resulted in the formation of Tumors with histological features of poorly differentiated HCC. Taken together, our findings indicate that forced expression of Bmi1 promotes the malignant transformation of HPCs, suggesting Bmi1 might be a potential molecular target for the treatment of HCC.
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Hepatocellular Carcinoma With Bile Duct Tumor Thrombus: A Clinicopathological Analysis of Factors Predictive of Recurrence and Outcome After Surgery
Medicine, 2015Co-Authors: Hong Zeng, Rui Zhang, Jian-ming Wen, Man-sheng Zhu, Xiang-de Shi, Chao LiuAbstract:AbstractAlthough hepatocellular carcinoma (HCC) with Bile Duct Tumor thrombus (BDTT) is a rare entity, most patients experience Tumor recurrence even after curative resection and the prognosis remains dismal. This study aimed to analyze the clinicopathological risk factors for recurrence and poor ou
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Pathological characteristics of Bile Duct Tumor thrombi and its influence on the prognosis of patients with hepatocellular carcinoma after surgical treatments
2014Co-Authors: Hong Zeng, Rui Zhang, Jian-ming Wen, Man-sheng ZhuAbstract:Objective To investigate the pathological characteristics of Bile Duct Tumor thrombi (BDTT) and its influence on the prognosis of patients with hepatocellular carcinoma (HCC) after surgical treatments. Methods Clinical data of 26 patients with HCC and BDTT (23 males, 3 females, age ranging from 23 to 78 years old and the median age of 52 years old) who underwent hepatectomy in Department of Hepatopancreatobiliary Surgery, Sun Yat-sen Memorial Hospital of Sun Yat-sen University from June 2007 to June 2012 were analyzed retrospectively. The informed consents of all patients were obtained and the ethical committee approval was received. The surgical procedures included regular hepatectomy (n=12), irregular hepatectomy (n=14). The treatments for BDTT included Bile Duct thrombectomy (n=13), extrahepatic Bile Duct resection (n=8), combined resection of BDTT and Tumor (n=5). The pathological characteristics of BDTT were observed and its correlation with Tumor recurrence was analyzed. The comparison of rates was conDucted by Fisher's exact probability test. Results Two cases of pure small microscopic BDTT and 24 cases of big macroscopic BDTT (14 cases were combined with pure small microscopic BDTT) were observed. According to the Satoh's BDTT clinical classification, there were 3 cases of typeⅠ, and 21 cases of type Ⅱ in 24 cases of big macroscopic BDTT. In all 26 cases of BDTT, sub-epithelium spreading along the Bile Duct wall was observed in 23 cases, most of which were small microscopic BDTT or big macroscopic BDTT. IntraDuctal spreading was rare and was observed mostly at the end part of big macroscopic BDTT. During the follow-up, intrahepatic Tumor recurrence was observed in 10 cases, in which 8 cases were combined with BDTT recurrence. The intrahepatic Tumor recurrence rate was 3/9 in patients after regular hepatectomy, and was 58% (7/12) in patients after irregular hepatectomy. In the 13 cases receiving Bile Duct thrombectomy, 7 cases suffered from BDTT recurrence with the recurrence rate of 54% (7/13). In the 8 cases receiving extrahepatic Bile Duct resection, 1 case suffered from BDTT recurrence with the recurrence rate of 1/8, where significant difference was observed (P
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Elevated expression of Bmi1 in hepatocellular carcinoma with Bile Duct Tumor thrombi.
Hepato-gastroenterology, 2013Co-Authors: Rui Zhang, Hong Zeng, Jie Wang, Chao LiuAbstract:BACKGROUND/AIMS Hepatocellular carcinoma (HCC) with Bile Duct Tumor thrombi (BDTT) is a rare event. The Bmi1 gene has been reported to play important roles in cancer initiation and progression. In this study, the expression of Bmi1 in HCC with BDTT was investigated. METHODOLOGY The expression of Bmi1 was examined immunohistochemically in HCC patients with BDTT (B+ group), HCC patients with vascular invasion (V+ group) and combined hepatocellular carcinoma and cholangiocarcinoma (C+ group), respectively. Besides, the Bmi1 mRNA level was investigated by real time PCR in the fresh samples obtained from 13 cases in B+ group, 10 cases in V+ group, and 6 cases in C+ group, respectively. RESULTS Immunohistochemical staining for Bmi1 showed Bmi1 was highly expressed in the B+ group in comparison with the C+ group and the V+ group, respectively. The Bmi1 mRNA level by real time PCR also showed that it was significantly up-regulated in B+ group compared with those of C+ group and V+ group, respectively. However, there was no statistically significant difference in Bmi1 levels between the subjects with vascular invasion and the subjects without vascular invasion. CONCLUSIONS Bmi1 might play important roles in the development of BDTT in HCC.