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Jiang Chang - One of the best experts on this subject based on the ideXlab platform.

  • regulation of immune response by bioactive ions released from silicate Bioceramics for bone regeneration
    Acta Biomaterialia, 2018
    Co-Authors: Yan Huang, Jiang Chang, Xiaoling Zhang, Kerong Dai
    Abstract:

    Abstract Silicate Bioceramics have been considered to possess a wide prospect of clinical application for orthopedic tissue regeneration due to their excellent osteogenesis and angiogenesis. However, the mechanism for silicate Bioceramics stimulating bone formation is not fully understood. The host immune defense to implants is proved to greatly influence the osteogenesis and new bone formation, but up to now, few studies are focused on the silicate Bioceramics modulated host immune responses. In our present study, two representative silicate Bioceramics, akermanite (AKT) and nagelschmidtite (NAGEL) were used as model materials to investigate the inflammation responses in vitro and in vivo, and β-tricalcium phosphate (β-TCP) Bioceramics were used as a control. It was found that the mouse macrophage cell RAW264.7 that cultured on AKT and NAGEL Bioceramics displayed not only less viability and proliferation, but also a significant less inflammatory cytokine secretion than those on β-TCP in vitro. The formation of foreign body giant cells and fibrous capsules, the invasion of macrophages, as well as the detected inflammatory cytokines around the implanted materials were much lower in both AKT and NAGEL bioceramic groups as compared with those in the β-TCP controls in vivo. Furthermore, it was found that not just the certain concentration of extracellular Si-containing ionic products released from the silicate Bioceramics, but also the separate Si, Mg and Ca ions revealed the activity to inhibit the macrophage inflammatory responses by the way of suppressing the activated inflammatory MAPK and NF-κB signaling pathway and promoting the caspase-dependent apoptosis of macrophages. In general, our study suggests that the silicate Bioceramics could regulate immune responses by altering the ionic microenvironment between the implants and hosts, which may offer new insight about the mechanism of the bioactivity of silicate Bioceramics in bone regeneration and provide profitable guidance for designing new biomaterials for bone tissue engineering. Statement of Significance Silicate Bioceramics have been widely used for orthopedic tissue regeneration because of their excellent characteristics in bone formation. However, there are few studies concerning their interrelationships with the host immune defense that has been proved to greatly influence osteogenesis. In our present study, the akermanite and nagelschmidtite were used as two representative silicate Bioceramics to investigate the inflammation responses in vitro and in vivo; and for the first time, the bioactive ions released from the silicate Bioceramics were discovered to regulate the macrophage immune responses through both inhibiting the inflammatory signaling and activating apoptosis of macrophages. Our findings in this study may not only increase the understanding in osteogenic activity of silicate Bioceramics, but also provide profitable guidance for designing and manufacturing new biomaterials for bone tissue engineering.

  • silicate based Bioceramics regulating osteoblast differentiation through a bmp2 signalling pathway
    Journal of Materials Chemistry B, 2017
    Co-Authors: Dong Zhai, Liqi Liu, Jiang Chang
    Abstract:

    Bioactive materials with osteostimulation properties have the potential to promote bone regeneration. We have found that silicate-based biomaterials have the osteostimulation ability for regeneration of large bone defects; however, the corresponding mechanism is unclear. In this study, we set out to elucidate the potential mechanism of silicate-based biomaterials with osteostimulation ability. A model silicate bioceramic, nagelschmidtite (NAGEL, Ca7P2Si2O16), was applied to study their ionic products on the effect of the Bone morphogenic protein (BMP) signaling pathway for osteoblast MC3T3-E1 as NAGEL has been previously shown to have excellent in vitro and in vivo bone-forming activity. BMP signaling, especially BMP2, is involved in bone formation during mammalian development and exhibits versatile regulatory functions in the body. It is found that NAGEL Bioceramics significantly enhance the migration and osteoblastic differentiation of MC3T3-E1. mRNA and protein expression of BMP2 is enhanced by NAGEL Bioceramics in a dose-dependent manner. Moreover, NAGEL Bioceramics activate the Smad-dependent BMP signaling pathway and induce the activation of the BMP downstream cascade (OCN, OPN and Runx2). The accumulation of phosphorylated-Smad1/5 is induced by NAGEL Bioceramics in the MC3T3-E1 cell nucleus. It is further found that NAGEL bioceramic-mediated migration, osteoblastic differentiation and the activation of the BMP downstream cascade are significantly downregulated by inhibition of BMP2 activity. Our results suggest that silicate-based NAGEL Bioceramics possess excellent in vitro osteostimulation properties and the possible mechanism of silicate-based biomaterials with distinct osteostimulation may be directly related to the activation of the BMP2 signaling pathway of osteoblasts by release of Si-containing bioactive ionic products.

  • injectable bioactive akermanite alginate composite hydrogels for in situ skin tissue engineering
    Journal of Materials Chemistry B, 2017
    Co-Authors: Yan Han, Jiang Chang, Qiongyu Zeng, Jinliang Peng
    Abstract:

    In situ tissue engineering holds great promise in regenerative medicine owing to the utilization of the body's own regenerative capacity via recruiting host endogenous stem cells or tissue-specific progenitor cells to the site of injury. In this study, an injectable bioactive akermanite/alginate composite hydrogel was prepared for in situ tissue engineering using an akermanite bioceramic as a bioactive cross-linking component to provide bioactive ions such as Ca, Mg and Si. These bioactive ions on the one hand cross-link alginate to form injectable hydrogels in the presence of acidic amino acids and on the other hand function as bioactive stimuli to activate the wound healing process. The bioactive hydrogel exhibits specific activity in regulating cell behavior such as migration, proliferation and differentiation both in vitro and in vivo. Most interestingly, using a chronic wound healing model, we demonstrated for the first time that the composite hydrogel significantly enhances the healing of chronic wounds by recruiting stem cells, stimulating cell proliferation, and enhancing blood vessel formation and re-epithelialization. Our results indicate that the injectable bioactive composite hydrogels act as in situ tissue engineering scaffolds to stimulate the regeneration of skin tissue, and utilizing the interaction between the bioactive Bioceramics and biopolymers, in which Bioceramics function as both cross-linking agents and bioactive factors, is a versatile strategy for designing multifunctional bioactive biomaterials for wound healing and tissue engineering applications.

  • Preparation and in vitro osteogenic, angiogenic and antibacterial properties of cuprorivaite (CaCuSi4O10, Cup) Bioceramics
    RSC Advances, 2016
    Co-Authors: Tian Tian, Chengtie Wu, Jiang Chang
    Abstract:

    For better tissue regeneration, the combination of beneficial inorganic ions may provide a more effective and safer strategy to couple with multi-functions (such as osteogenesis, angiogenesis and antibacterial activity) as compared with growth factors. Previous studies have demonstrated that Si ions released from silicate Bioceramics could promote osteogenesis and angiogenesis. Divalent copper ions (Cu2+) were widely reported to enhance angiogenesis and possess antibacterial activity. Therefore, we hypothesize that the combination of Cu and Si ions may reveal better osteogenic, angiogenic and antibacterial activity. In this study, we synthesized a Cu-containing silicate bioceramic, cuprorivaite (CaCuSi4O10, Cup) and investigated its in vitro osteogenic, angiogenic and antibacterial capacities. We, for the first time, successfully synthesized Cup powders with high-purity by sol–gel method. In vitro biological evaluation indicated that ionic dissolution products of Cup ceramics in the concentration range of 0.195–0.78 mg mL−1 were able to significantly promote angiogenesis. More importantly, Si and Cu ions had a synergistic effect on angiogenesis at lower concentrations compared with those reported in the literature. Moreover, Cup ceramics had good antibacterial activity. However, ionic dissolution products of Cup ceramics at 0.195–0.78 mg mL−1 notably suppressed ALP activity of osteoblast-like cells. Therefore, Cup Bioceramics may have a potential application in wound healing, but may not be suitable for bone regeneration.

  • effect of micro nano hybrid structured hydroxyapatite Bioceramics on osteogenic and cementogenic differentiation of human periodontal ligament stem cell via wnt signaling pathway
    International Journal of Nanomedicine, 2015
    Co-Authors: Lixia Mao, Lingyong Jiang, Jiang Chang, Jiaqiang Liu, Lunguo Xia, Jinglei Zhao, Xiuhui Wang, Kaili Lin, Bing Fang
    Abstract:

    The surface structure of bioceramic scaffolds is crucial for its bioactivity and osteoinductive ability, and in recent years, human periodontal ligament stem cells have been certified to possess high osteogenic and cementogenic differential ability. In the present study, hydroxyapatite (HA) Bioceramics with micro-nano-hybrid surface (mnHA [the hybrid of nanorods and microrods]) were fabricated via hydrothermal reaction of the α-tricalcium phosphate granules as precursors in aqueous solution, and the effects of mnHA on the attachment, proliferation, osteogenic and cementogenic differentiations of human periodontal ligament stem cells as well as the related mechanisms were systematically investigated. The results showed that mnHA Bioceramics could promote cell adhesion, proliferation, alkaline phosphatase (ALP) activity, and expression of osteogenic/cementogenic-related markers including runt-related transcription factor 2 (Runx2), ALP, osteocalcin (OCN), cementum attachment protein (CAP), and cementum protein (CEMP) as compared to the HA Bioceramics with flat and dense surface. Moreover, mnHA Bioceramics stimulated gene expression of low-density lipoprotein receptor-related protein 5 (LRP5) and β-catenin, which are the key genes of canonical Wnt signaling. Moreover, the stimulatory effect on ALP activity and osteogenic and cementogenic gene expression, including that of ALP, OCN, CAP, CEMP, and Runx2 of mnHA Bioceramics could be repressed by canonical Wnt signaling inhibitor dickkopf1 (Dkk1). The results suggested that the HA Bioceramics with mnHA could act as promising grafts for periodontal tissue regeneration.

Bing Fang - One of the best experts on this subject based on the ideXlab platform.

  • the synergistic effects of sr and si bioactive ions on osteogenesis osteoclastogenesis and angiogenesis for osteoporotic bone regeneration
    Acta Biomaterialia, 2017
    Co-Authors: Lingyong Jiang, Chengtie Wu, Bing Fang
    Abstract:

    Abstract Bioactive ions released from Bioceramics play important roles in bone regeneration; however, it is unclear how each ionic composition in complex Bioceramics exerts its specific effect on bone regeneration. The aim of this study is to elucidate the functional effects of Sr and Si ions in Bioceramics on the regeneration of osteoporotic bone. A model bioceramic with Sr- and Si-containing components (SMS) was successfully fabricated and the effects of ionic products from SMS Bioceramics on the osteogenic, osteoclastic and angiogenic differentiation of rBMSCs-OVX and RANKL-induced osteoclasts were investigated. The results showed that SMS Bioceramics could enhance ALP activity and expression of Col 1, OCN, Runx2, and angiogenic factors including VEGF and Ang-1. SMS Bioceramics not only rebalanced the OPG/RANKL ratio of rBMSCs-OVX at early stage, but also repressed RANKL-induced osteoclast formation and expression of TRAP, DC-STAMP, V-ATPase a3, and NFATc1. The synergistic effects of Sr and Si ions were further investigated as compared with those of similar concentrations of Sr and Si ions alone. Sr and Si ions possessed synergistic effects on osteogenesis, osteoclastogenesis, and angiogenesis, attributed to the dominant effects of Sr ions on enhancing angiogenesis and repressing osteoclastogenesis, and the dominant effects of Si ions on stimulating osteogenesis. The in vivo study using critical-size mandibular defects of OVX rat models showed that SMS Bioceramics could significantly enhance bone formation and mineralization compared with β-TCP Bioceramics. Our results are the first to elucidate the specific effect of each ion from Bioceramics on osteogenesis, osteoclastogenesis and angiogenesis for osteoporotic bone regeneration, paving the way for the design of functional biomaterials with complex compositions for tissue engineering and regenerative medicine. Statement of significance Bioactive ions released from Bioceramics play important roles for bone regeneration; however, it is unclear how each of ionic compositions in complex Bioceramics exerts its specific effect on bone regeneration. The aim of present study is to elucidate the functional effects of Sr and Si ions in complex Bioceramics on the regeneration of osteoporotic bone. A model bioceramic with Sr and Si-containing components (SMS) was successfully fabricated and the effects of ionic products from SMS Bioceramics on the osteogenic, osteoclastic and angiogenic differentiation of rBMSCs-OVX and RANKL-induced osteoclasts were investigated. The results showed that SMS Bioceramics could enhance ALP activity and expression of Col 1, OCN, Runx2 and angiogenic factors including VEGF and Ang-1. SMS Bioceramics not only rebalanced the ratio of OPG/RANKL of OVX-BMSCs at early stage, but also repressed RANKL-induced osteoclast formation and expression of TRAP, DC-STAMP, V-ATPase a3, and NFATc1. The synergistic effects of Sr and Si ions were further investigated as compared with the similar concentration of Sr and Si ions alone. It was found that Sr and Si ions possessed synergistic effects on osteogenesis, osteoclastogenesis and angiogenesis, attributed to the dominant effects of Sr ions on enhancing angiogenesis and repressing osteoclastogenesis, and the dominant effects of Si ions on stimulating osteogenesis. The in vivo study using critical-size mandibular defects of OVX rat models showed that SMS Bioceramics could significantly enhance bone formation and mineralization as compared with β-TCP Bioceramics. It is suggested that SMS Bioceramics may be a promising biomaterial for osteoporotic bone regeneration. To our knowledge, this is the first time to elucidate the specific effect of each ion from Bioceramics on osteogenesis, osteoclastogenesis and angiogenesis for osteoporotic bone regeneration, paving the way to design functional biomaterials with complex compositions for tissue engineering and regenerative medicine.

  • effect of micro nano hybrid structured hydroxyapatite Bioceramics on osteogenic and cementogenic differentiation of human periodontal ligament stem cell via wnt signaling pathway
    International Journal of Nanomedicine, 2015
    Co-Authors: Lixia Mao, Lingyong Jiang, Jiang Chang, Jiaqiang Liu, Lunguo Xia, Jinglei Zhao, Xiuhui Wang, Kaili Lin, Bing Fang
    Abstract:

    The surface structure of bioceramic scaffolds is crucial for its bioactivity and osteoinductive ability, and in recent years, human periodontal ligament stem cells have been certified to possess high osteogenic and cementogenic differential ability. In the present study, hydroxyapatite (HA) Bioceramics with micro-nano-hybrid surface (mnHA [the hybrid of nanorods and microrods]) were fabricated via hydrothermal reaction of the α-tricalcium phosphate granules as precursors in aqueous solution, and the effects of mnHA on the attachment, proliferation, osteogenic and cementogenic differentiations of human periodontal ligament stem cells as well as the related mechanisms were systematically investigated. The results showed that mnHA Bioceramics could promote cell adhesion, proliferation, alkaline phosphatase (ALP) activity, and expression of osteogenic/cementogenic-related markers including runt-related transcription factor 2 (Runx2), ALP, osteocalcin (OCN), cementum attachment protein (CAP), and cementum protein (CEMP) as compared to the HA Bioceramics with flat and dense surface. Moreover, mnHA Bioceramics stimulated gene expression of low-density lipoprotein receptor-related protein 5 (LRP5) and β-catenin, which are the key genes of canonical Wnt signaling. Moreover, the stimulatory effect on ALP activity and osteogenic and cementogenic gene expression, including that of ALP, OCN, CAP, CEMP, and Runx2 of mnHA Bioceramics could be repressed by canonical Wnt signaling inhibitor dickkopf1 (Dkk1). The results suggested that the HA Bioceramics with mnHA could act as promising grafts for periodontal tissue regeneration.

  • designing ordered micropatterned hydroxyapatite Bioceramics to promote the growth and osteogenic differentiation of bone marrow stromal cells
    Journal of Materials Chemistry B, 2015
    Co-Authors: Cancan Zhao, Dong Zhai, Jiang Chang, Jiaqiang Liu, Lunguo Xia, Bing Fang, Na Zhang, Kaili Lin
    Abstract:

    Patterned structured surfaces are very useful to control a cell's microenvironment and to modulate certain cellular responses, such as cell adhesion, migration, proliferation, and differentiation. Herein, ordered micropatterns constructed by a quadrate convex with different sizes were fabricated on a hydroxyapatite [Ca10(PO4)6(OH)2, HAp] bioceramic surface using an ordered micropatterned nylon sieve as templates. The height, width and space of the convex for the patterns could be facilely regulated via simply tailoring the meshes of the template. Compared with traditional samples with flat surfaces, the fabricated HAp Bioceramics with micropatterned surfaces possessed better wettability and higher surface energy, which significantly promoted the adhesion, proliferation, and osteogenic differentiation of rat bone marrow stromal cells (bMSCs). Furthermore, using a pattern size close to that of the cell size showed a better stimulation of cell response compared with larger pattern sizes. Our study suggests that the fabrication of micropatterned structured HAp Bioceramics is critical for designing optimal biomaterials for bone regeneration and cell culture substrate applications.

Kerong Dai - One of the best experts on this subject based on the ideXlab platform.

  • regulation of immune response by bioactive ions released from silicate Bioceramics for bone regeneration
    Acta Biomaterialia, 2018
    Co-Authors: Yan Huang, Jiang Chang, Xiaoling Zhang, Kerong Dai
    Abstract:

    Abstract Silicate Bioceramics have been considered to possess a wide prospect of clinical application for orthopedic tissue regeneration due to their excellent osteogenesis and angiogenesis. However, the mechanism for silicate Bioceramics stimulating bone formation is not fully understood. The host immune defense to implants is proved to greatly influence the osteogenesis and new bone formation, but up to now, few studies are focused on the silicate Bioceramics modulated host immune responses. In our present study, two representative silicate Bioceramics, akermanite (AKT) and nagelschmidtite (NAGEL) were used as model materials to investigate the inflammation responses in vitro and in vivo, and β-tricalcium phosphate (β-TCP) Bioceramics were used as a control. It was found that the mouse macrophage cell RAW264.7 that cultured on AKT and NAGEL Bioceramics displayed not only less viability and proliferation, but also a significant less inflammatory cytokine secretion than those on β-TCP in vitro. The formation of foreign body giant cells and fibrous capsules, the invasion of macrophages, as well as the detected inflammatory cytokines around the implanted materials were much lower in both AKT and NAGEL bioceramic groups as compared with those in the β-TCP controls in vivo. Furthermore, it was found that not just the certain concentration of extracellular Si-containing ionic products released from the silicate Bioceramics, but also the separate Si, Mg and Ca ions revealed the activity to inhibit the macrophage inflammatory responses by the way of suppressing the activated inflammatory MAPK and NF-κB signaling pathway and promoting the caspase-dependent apoptosis of macrophages. In general, our study suggests that the silicate Bioceramics could regulate immune responses by altering the ionic microenvironment between the implants and hosts, which may offer new insight about the mechanism of the bioactivity of silicate Bioceramics in bone regeneration and provide profitable guidance for designing new biomaterials for bone tissue engineering. Statement of Significance Silicate Bioceramics have been widely used for orthopedic tissue regeneration because of their excellent characteristics in bone formation. However, there are few studies concerning their interrelationships with the host immune defense that has been proved to greatly influence osteogenesis. In our present study, the akermanite and nagelschmidtite were used as two representative silicate Bioceramics to investigate the inflammation responses in vitro and in vivo; and for the first time, the bioactive ions released from the silicate Bioceramics were discovered to regulate the macrophage immune responses through both inhibiting the inflammatory signaling and activating apoptosis of macrophages. Our findings in this study may not only increase the understanding in osteogenic activity of silicate Bioceramics, but also provide profitable guidance for designing and manufacturing new biomaterials for bone tissue engineering.

  • in vitro and in vivo evaluation of akermanite Bioceramics for bone regeneration
    Biomaterials, 2009
    Co-Authors: Yan Huang, Jiang Chang, Xiaogang Jin, Xiaoling Zhang, Hongli Sun, Tingting Tang, Kerong Dai
    Abstract:

    Abstract This study investigated the effects of a calcium magnesium silicate bioceramic (akermanite) for bone regeneration in vitro and in vivo, with β-tricalcium phosphate (β-TCP) as a control. In vitro, the human bone marrow-derived mesenchymal stromal cells (hBMSCs) were cultured in an osteogenic medium supplemented with a certain concentration of two Bioceramics' extracts for 20 days. An MTT assay showed that akermanite extract promoted proliferation of hBMSC significantly more than did β-TCP extract. The results of alkaline phosphatase (ALP) activity test and the expression of osteogenic marker genes such as ALP, osteopontin (OPN), osteocalcin (OCN) and bone sialoprotein (BSP) demonstrated that the osteogenic differentiation of hBMSC was enhanced more by akermanite extract than by β-TCP extract. In vivo, a histomorphology analysis and histomorphometry of the two porous Bioceramics implants in rabbit femur defect models indicated that both in early- and late-stage implantations, akermanite promoted more osteogenesis and biodegradation than did β-TCP; and in late-stage implantations, the rate of new bone formation was faster in akermanite than in β-TCP. These results suggest that akermanite might be a potential and attractive bioceramic for tissue engineering.

Jiao Sun - One of the best experts on this subject based on the ideXlab platform.

  • odontogenic differentiation of human dental pulp cells induced by silicate based Bioceramics via activation of p38 mepe pathway
    RSC Advances, 2015
    Co-Authors: Xiao-meng Zhang, Jiang Chang, Jiao Sun
    Abstract:

    To investigate whether silicate-based Bioceramics have an odontogenic function, researchers mainly study the odontogenic differentiation potential of human dental pulp cells (hDPCs) cultured with the silicate-based Ca7Si2P2O16 (CSP) bioceramic extracts and the underlying mechanism. Firstly, the effects of CSP extracts on proliferation and odontogenic differentiation of hDPCs were studied. The dentine-related protein expression stimulated by CSP extracts was investigated and the influence of P38/MEPE pathway in this process was further explored. The results showed that CSP Bioceramics not only presented good cytocompatibility with hDPCs, but also promoted the odontogenic gene and protein expression (DSPP, DMP1, OPN and RUNX2) of hDPCs. Western blot results further indicated that the possible mechanism might be related to the activation of P38/MEPE pathway. In summary, our findings suggest that CSP Bioceramics can induce the odontogenic differentiation of hDPCs, offering essential evidence for the potential application of silicate-based Bioceramics as pulp capping materials or additives.

  • Odontogenic differentiation of human dental pulp cells induced by silicate-based Bioceramics via activation of P38/MEPE pathway
    RSC Advances, 2015
    Co-Authors: Xiao-meng Zhang, Jiang Chang, Jiao Sun
    Abstract:

    To investigate whether silicate-based Bioceramics have an odontogenic function, researchers mainly study the odontogenic differentiation potential of human dental pulp cells (hDPCs) cultured with the silicate-based Ca7Si2P2O16 (CSP) bioceramic extracts and the underlying mechanism. Firstly, the effects of CSP extracts on proliferation and odontogenic differentiation of hDPCs were studied. The dentine-related protein expression stimulated by CSP extracts was investigated and the influence of P38/MEPE pathway in this process was further explored. The results showed that CSP Bioceramics not only presented good cytocompatibility with hDPCs, but also promoted the odontogenic gene and protein expression (DSPP, DMP1, OPN and RUNX2) of hDPCs. Western blot results further indicated that the possible mechanism might be related to the activation of P38/MEPE pathway. In summary, our findings suggest that CSP Bioceramics can induce the odontogenic differentiation of hDPCs, offering essential evidence for the potential application of silicate-based Bioceramics as pulp capping materials or additives.

Cheng Jack Chun-yiu - One of the best experts on this subject based on the ideXlab platform.

  • Novel approach for quantification of porosity for biomaterial implants using microcomputed tomography (μCT)
    Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2005
    Co-Authors: Yeung Hiu-yan, Qin Ling, Lee Kwong-man, Zhang Ming, Leung Kwok-sui, Cheng Jack Chun-yiu
    Abstract:

    Porous Bioceramics have been widely investigated in orthopaedic tissue engineering. Attention has been given to manufacturing of a porous bioceramic that mimics the trabecular bone structure for proper bone regeneration. With the advance of biomedical imaging through microcomputed tomography (μCT), this study attempted to quantify the pore structure of different Bioceramics. Two bioceramic blocks (BSC and ChronOS) were synthesized by two methods. The specification claimed the porosity of the bioceramic ranged from 40% to 70%. Six blocks of each bioceramic were evaluated by conventional water immersion method and μCT. The pore size and connectivity were evaluated with standardized protocols. By the water immersion method, the porosity of BSC and ChronOS was 60.4% and 74.7%, respectively. The three-dimensional results of μCT showed that BSC porosity was 26.2% and ChronOS was 60.0%. The pore connectivity was evaluated to be 2.6 for BSC and 39.7 for ChronOS. ChronOS had functional pores with 200 μm to 400 μm in diameter (87.8% ± 0.5%), which is significantly more than 52.8% ± 11.5% of pores in BSC (p < 0.05). Providing information on the functional pores objectively, the μCT evaluation serves as a good standard for specification of the bioceramic-related implants.Department of Rehabilitation Science

  • Novel approach for quantification of porosity for biomaterial implants using microcomputed tomography (μCT)
    Journal of biomedical materials research. Part B Applied biomaterials, 2005
    Co-Authors: Yeung Hiu-yan, Qin Ling, Lee Kwong-man, Zhang Ming, Leung Kwok-sui, Cheng Jack Chun-yiu
    Abstract:

    Porous Bioceramics have been widely investigated in orthopaedic tissue engineering. Attention has been given to manufacturing of a porous bioceramic that mimics the trabecular bone structure for proper bone regeneration. With the advance of biomedical imaging through microcomputed tomography (μCT), this study attempted to quantify the pore structure of different Bioceramics. Two bioceramic blocks (BSC and ChronOS) were synthesized by two methods. The specification claimed the porosity of the bioceramic ranged from 40% to 70%. Six blocks of each bioceramic were evaluated by conventional water immersion method and μCT. The pore size and connectivity were evaluated with standardized protocols. By the water immersion method, the porosity of BSC and ChronOS was 60.4% and 74.7%, respectively. The three-dimensional results of μCT showed that BSC porosity was 26.2% and ChronOS was 60.0%. The pore connectivity was evaluated to be 2.6 for BSC and 39.7 for ChronOS. ChronOS had functional pores with 200 μm to 400 μm in diameter (87.8% ± 0.5%), which is significantly more than 52.8% ± 11.5% of pores in BSC (p < 0.05). Providing information on the functional pores objectively, the μCT evaluation serves as a good standard for specification of the bioceramic-related implants. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2005