The Experts below are selected from a list of 18315 Experts worldwide ranked by ideXlab platform

Changsheng Zhao - One of the best experts on this subject based on the ideXlab platform.

  • improving the blood compatibility of material surfaces via Biomolecule immobilized mussel inspired coatings
    Journal of Biomedical Materials Research Part A, 2011
    Co-Authors: Beijia Li, Nan Yi, Baihai Su, Fulong Zhang, Jie Li, Changsheng Zhao
    Abstract:

    In this article, we presented a general protocol to prepare Biomolecule-immobilized mussel-inspired polydopamine (PDA) coatings to improve the blood compatibility of broad ranges of material surfaces. It needs only a simple immersion of substrates in dopamine solution at alkaline pH to form mussel-inspired PDA coating, and then immersing the PDA coated substrates into Biomolecule solution to conjugate Biomolecules. XPS, water contact angle analysis, and protein assay confirmed that Biomolecules could be successfully coated on several material surfaces, including nylon, cellulose, and polyethersulfone membrane surfaces. For the protein fouling resistance, the bovine serum albumin (BSA) modified surfaces were more effective than the amino acid modified surfaces. And the platelet adhesion on the BSA-modified material surfaces was obviously depressed. These results indicated that the blood compatibility of the surfaces was improved by the biomacromolecule-immobilized mussel-inspired coating which might be considered as a universal coating to modify a wide variety of materials. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.

  • improving the blood compatibility of material surfaces via Biomolecule immobilized mussel inspired coatings
    Journal of Biomedical Materials Research Part A, 2011
    Co-Authors: Qiang Wei, Fulong Zhang, Zehua Yin, Changsheng Zhao
    Abstract:

    In this article, we presented a general protocol to prepare Biomolecule-immobilized mussel-inspired polydopamine (PDA) coatings to improve the blood compatibility of broad ranges of material surfaces. It needs only a simple immersion of substrates in dopamine solution at alkaline pH to form mussel-inspired PDA coating, and then immersing the PDA coated substrates into Biomolecule solution to conjugate Biomolecules. XPS, water contact angle analysis, and protein assay confirmed that Biomolecules could be successfully coated on several material surfaces, including nylon, cellulose, and polyethersulfone membrane surfaces. For the protein fouling resistance, the bovine serum albumin (BSA) modified surfaces were more effective than the amino acid modified surfaces. And the platelet adhesion on the BSA-modified material surfaces was obviously depressed. These results indicated that the blood compatibility of the surfaces was improved by the biomacromolecule-immobilized mussel-inspired coating which might be considered as a universal coating to modify a wide variety of materials.

Itamar Willner - One of the best experts on this subject based on the ideXlab platform.

  • Biomolecule nanomaterial hybrid systems for nanobiotechnology
    Advances in Experimental Medicine and Biology, 2012
    Co-Authors: Ran Telvered, Omer Yehezkeli, Itamar Willner
    Abstract:

    The integration of Biomolecules with metallic or semiconductor nanoparticles or carbon nanotubes yields new hybrid nanostructures of unique features that combine the properties of the Biomolecules and of the nano-elements. These unique features of the hybrid Biomolecule/nanoparticle systems provide the basis for the rapid development of the area of nanobiotechnology. Recent advances in the implementation of hybrid materials consisting of Biomolecules and metallic nanoparticles or semiconductor quantum dots will be discussed. The following topics will be exemplified: (i) The electrical wiring of redox enzymes with electrodes by means of metallic nanoparticles or carbon nanotubes, and the application of the modified electrodes as amperometric biosensors or for the construction of biofuel cells. (ii) The biocatalytic growth of metallic nanoparticles as a means to construct optical or electrical sensors. (iii) The functionalization of semiconductor quantum dots with Biomolecules and the application of the hybrid nanostructures for developing different optical sensors, including intracellular sensor systems. (iv) The use of Biomolecule-metallic nanoparticle nanostructures as templates for growing metallic nanowires, and the construction of fuel-driven nano-transporters.

  • Biomolecule-based nanomaterials and nanostructures
    Nano Letters, 2010
    Co-Authors: Itamar Willner, Bilha Willner
    Abstract:

    Biomolecule-nanoparticle (or carbon nanotube) hybrid systems provide new materials that combine the unique optical, electronic, or catalytic properties of the nanoelements with the recognition or biocatalytic functions of Biomolecules. This article summarizes recent applications of Biomolecule-nanoparticle (or carbon nanotubes) hybrid systems for sensing, synthesis of nanostructures, and for the fabrication of nanoscale devices. The use of metallic nanoparticles for the electrical contacting of redox enzymes with electrodes, and as catalytic labels for the development of electrochemical biosensors is discussed. Similarly, Biomolecule-quantum dot hybrid systems are implemented for optical biosensing, and for monitoring intracellular metabolic processes. Also, the self-assembly of Biomolecule-metal nanoparticle hybrids into nanostructures and functional nanodevices is presented. The future perspectives of the field are addressed by discussing future challenges and highlighting different potential applications.

  • nanoparticle enzyme hybrid systems for nanobiotechnology
    FEBS Journal, 2007
    Co-Authors: Itamar Willner, Bernhard Basnar, Bilha Willner
    Abstract:

    Biomolecule–nanoparticle (NP) [or quantum-dot (QD)] hybrid systems combine the recognition and biocatalytic properties of Biomolecules with the unique electronic, optical, and catalytic features of NPs and yield composite materials with new functionalities. The Biomolecule–NP hybrid systems allow the development of new biosensors, the synthesis of metallic nanowires, and the fabrication of nanostructured patterns of metallic or magnetic NPs on surfaces. These advances in nanobiotechnology are exemplified by the development of amperometric glucose sensors by the electrical contacting of redox enzymes by means of AuNPs, and the design of an optical glucose sensor by the biocatalytic growth of AuNPs. The biocatalytic growth of metallic NPs is used to fabricate Au and Ag nanowires on surfaces. The fluorescence properties of semiconductor QDs are used to develop competitive maltose biosensors and to probe the biocatalytic functions of proteases. Similarly, semiconductor NPs, associated with electrodes, are used to photoactivate bioelectrocatalytic cascades while generating photocurrents.

  • Integrated nanoparticle-Biomolecule hybrid systems: synthesis, properties, and applications
    Angewandte Chemie-International Edition, 2004
    Co-Authors: Eugenii Katz, Itamar Willner
    Abstract:

    Nanomaterials, such as metal or semiconductor nanoparticles and nanorods, exhibit similar dimensions to those of Biomolecules, such as proteins (enzymes, antigens, antibodies) or DNA. The integration of nanoparticles, which exhibit unique electronic, photonic, and catalytic properties, with biomaterials, which display unique recognition, catalytic, and inhibition properties, yields novel hybrid nanobiomaterials of synergetic properties and functions. This review describes recent advances in the synthesis of Biomolecule-nanoparticle/nanorod hybrid systems and the application of such assemblies in the generation of 2D and 3D ordered structures in solutions and on surfaces. Particular emphasis is directed to the use of Biomolecule-nanoparticle (metallic or semiconductive) assemblies for bioanalytical applications and for the fabrication of bioelectronic devices.

Molly S Shoichet - One of the best experts on this subject based on the ideXlab platform.

  • a hyaluronan methylcellulose based hydrogel for local cell and Biomolecule delivery to the central nervous system
    Brain Research Bulletin, 2019
    Co-Authors: Carter J. Teal, Molly S Shoichet
    Abstract:

    Abstract Regenerative medicine strategies rely on exogenous cell transplantation and/or endogenous cell stimulation. Biomaterials can help to increase the regenerative potential of cells and Biomolecules by controlling transplanted cell fate and provide a local, sustained release of Biomolecules. In this review, we describe the use of a hyaluronan/methylcellulose (HAMC)-based hydrogel as a delivery vehicle to the brain, spinal cord, and retina to promote cellular survival and tissue repair. We discuss various controlled release strategies to prolong the delivery of factors for neuroprotection. The versatility of this hydrogel for a diversity of applications highlights its potential to enhance cell- and Biomolecule-based treatment strategies.

  • cell and Biomolecule delivery for tissue repair and regeneration in the central nervous system
    Journal of Controlled Release, 2014
    Co-Authors: Irja Elliott Donaghue, Michael V Sefton, Molly S Shoichet
    Abstract:

    Abstract Tissue engineering frequently involves cells and scaffolds to replace damaged or diseased tissue. It originated, in part, as a means of effecting the delivery of Biomolecules such as insulin or neurotrophic factors, given that cells are constitutive producers of such therapeutic agents. Thus cell delivery is intrinsic to tissue engineering. Controlled release of Biomolecules is also an important tool for enabling cell delivery since the Biomolecules can enable cell engraftment, modulate inflammatory response or otherwise benefit the behavior of the delivered cells. We describe advances in cell and Biomolecule delivery for tissue regeneration, with emphasis on the central nervous system (CNS). In the first section, the focus is on encapsulated cell therapy. In the second section, the focus is on Biomolecule delivery in polymeric nano/microspheres and hydrogels for the nerve regeneration and endogenous cell stimulation. In the third section, the focus is on combination strategies of neural stem/progenitor cell or mesenchymal stem cell and Biomolecule delivery for tissue regeneration and repair. In each section, the challenges and potential solutions associated with delivery to the CNS are highlighted.

Fulong Zhang - One of the best experts on this subject based on the ideXlab platform.

  • improving the blood compatibility of material surfaces via Biomolecule immobilized mussel inspired coatings
    Journal of Biomedical Materials Research Part A, 2011
    Co-Authors: Beijia Li, Nan Yi, Baihai Su, Fulong Zhang, Jie Li, Changsheng Zhao
    Abstract:

    In this article, we presented a general protocol to prepare Biomolecule-immobilized mussel-inspired polydopamine (PDA) coatings to improve the blood compatibility of broad ranges of material surfaces. It needs only a simple immersion of substrates in dopamine solution at alkaline pH to form mussel-inspired PDA coating, and then immersing the PDA coated substrates into Biomolecule solution to conjugate Biomolecules. XPS, water contact angle analysis, and protein assay confirmed that Biomolecules could be successfully coated on several material surfaces, including nylon, cellulose, and polyethersulfone membrane surfaces. For the protein fouling resistance, the bovine serum albumin (BSA) modified surfaces were more effective than the amino acid modified surfaces. And the platelet adhesion on the BSA-modified material surfaces was obviously depressed. These results indicated that the blood compatibility of the surfaces was improved by the biomacromolecule-immobilized mussel-inspired coating which might be considered as a universal coating to modify a wide variety of materials. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.

  • improving the blood compatibility of material surfaces via Biomolecule immobilized mussel inspired coatings
    Journal of Biomedical Materials Research Part A, 2011
    Co-Authors: Qiang Wei, Fulong Zhang, Zehua Yin, Changsheng Zhao
    Abstract:

    In this article, we presented a general protocol to prepare Biomolecule-immobilized mussel-inspired polydopamine (PDA) coatings to improve the blood compatibility of broad ranges of material surfaces. It needs only a simple immersion of substrates in dopamine solution at alkaline pH to form mussel-inspired PDA coating, and then immersing the PDA coated substrates into Biomolecule solution to conjugate Biomolecules. XPS, water contact angle analysis, and protein assay confirmed that Biomolecules could be successfully coated on several material surfaces, including nylon, cellulose, and polyethersulfone membrane surfaces. For the protein fouling resistance, the bovine serum albumin (BSA) modified surfaces were more effective than the amino acid modified surfaces. And the platelet adhesion on the BSA-modified material surfaces was obviously depressed. These results indicated that the blood compatibility of the surfaces was improved by the biomacromolecule-immobilized mussel-inspired coating which might be considered as a universal coating to modify a wide variety of materials.

Amitava Bhattacharyya - One of the best experts on this subject based on the ideXlab platform.

  • effect of nanocomposite coating and Biomolecule functionalization on silk fibroin based conducting 3d braided scaffolds for peripheral nerve tissue engineering
    Nanomedicine: Nanotechnology Biology and Medicine, 2020
    Co-Authors: Mamatha M Pillai, Sathish G Kumar, Shadi Houshyar, Rajiv Padhye, Amitava Bhattacharyya
    Abstract:

    Abstract In this work, the effects of carbon nanofiber (CNF) dispersed poly-e-caprolactone (PCL) nanocomposite coatings and Biomolecules functionalization on silk fibroin based conducting braided nerve conduits were studied for enhancing Neuro 2a cellular activities. A unique combination of Biomolecules (UCM) and varying concentrations of CNF (5, 7.5, 10% w/w) were dispersed in 10% (w/v) PCL solution for coating on degummed silk threads. The coated silk threads were braided to develop the scaffold structure. As the concentration of CNF increased in the coating, the electrical impedance decreased up to 400 Ω indicating better conductivity. The tensile and dynamic mechanical property analysis showed better mechanical properties in CNF coated samples. In vitro cytocompatibility analysis proved the non-toxicity of the developed braided conduits. Cell attachment, growth and proliferation were significantly enhanced on the Biomolecule functionalized nanocomposite coated silk braided structure, exhibiting their potential for peripheral nerve regeneration and recovery.