The Experts below are selected from a list of 49977 Experts worldwide ranked by ideXlab platform
Martin Pumera - One of the best experts on this subject based on the ideXlab platform.
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graphene in Biosensing
Materials Today, 2011Co-Authors: Martin PumeraAbstract:Biosensing is paramount for improving the quality of human life. Biosensors and Biosensing protocols are able to detect a wide range of compounds, sensitively and selectively, with applications in security, health care for point-of-care analyses of diseases, and environmental safety. Here, we describe biosensors and Biosensing systems employing graphene. Graphene is a zero-gap semiconductor material, which is electroactive and transparent. Because of its interesting properties, graphene has found its way into a wide variety of Biosensing schemes. It has been used as a transducer in bio-field-effect transistors, electrochemical biosensors, impedance biosensors, electrochemiluminescence, and fluorescence biosensors, as well as biomolecular labels. In our review, we describe the application of graphene for enzymatic Biosensing, DNA sensing, and immunosensing. We compare different techniques and present our views on the future development of the field.
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Graphene in Biosensing
Materials Today, 2011Co-Authors: Martin PumeraAbstract:Biosensing is paramount for improving the quality of human life. Biosensors and Biosensing protocols are able to detect a wide range of compounds, sensitively and selectively, with applications in security, health care for point-of-care analyses of diseases, and environmental safety. Here, we describe biosensors and Biosensing systems employing graphene. Graphene is a zero-gap semiconductor material, which is electroactive and transparent. Because of its interesting properties, graphene has found its way into a wide variety of Biosensing schemes. It has been used as a transducer in bio-field-effect transistors, electrochemical biosensors, impedance biosensors, electrochemiluminescence, and fluorescence biosensors, as well as biomolecular labels. In our review, we describe the application of graphene for enzymatic Biosensing, DNA sensing, and immunosensing. We compare different techniques and present our views on the future development of the field. © 2011 Elsevier Ltd.
Yongkang Ye - One of the best experts on this subject based on the ideXlab platform.
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gold nanoparticle based signal amplification for Biosensing
Analytical Biochemistry, 2011Co-Authors: Yongkang YeAbstract:Colloidal gold nanoparticles (AuNPs), with unique properties such as highly resonant particle plasmons, direct visualization of single nanoclusters by scattering of light, catalytic size enhancement by silver deposition, conductivity, and electrochemical properties, are very attractive materials for several applications in biotechnology. Furthermore, as excellent biological tags, AuNPs can be easily conjugated with biomolecules and retain the biochemical activity of the tagged biomolecules, making AuNPs ideal transducers for several biorecognition applications. The goal of this article is to review recent advances of using AuNPs as labels for signal amplification in Biosensing applications. We focus on the signal amplification strategies of AuNPs in Biosensing/biorecognition, more specifically, on the main optical and electrochemical detection methods that involve AuNP-based Biosensing. Particular attention is given to recent advances and trends in sensing applications.
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Gold nanoparticle-based signal amplification for Biosensing
Analytical Biochemistry, 2011Co-Authors: Xiaodong Cao, Yongkang Ye, Songqin LiuAbstract:Colloidal gold nanoparticles (AuNPs), with unique properties such as highly resonant particle plasmons, direct visualization of single nanoclusters by scattering of light, catalytic size enhancement by silver deposition, conductivity, and electrochemical properties, are very attractive materials for several applications in biotechnology. Furthermore, as excellent biological tags, AuNPs can be easily conjugated with biomolecules and retain the biochemical activity of the tagged biomolecules, making AuNPs ideal transducers for several biorecognition applications. The goal of this article is to review recent advances of using AuNPs as labels for signal amplification in Biosensing applications. We focus on the signal amplification strategies of AuNPs in Biosensing/biorecognition, more specifically, on the main optical and electrochemical detection methods that involve AuNP-based Biosensing. Particular attention is given to recent advances and trends in sensing applications. © 2011 Elsevier Inc. All rights reserved.
Frank Vollmer - One of the best experts on this subject based on the ideXlab platform.
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Advances in Single Molecule Biosensing
Sensors, 2015Co-Authors: Frank VollmerAbstract:In this presentation, we will report on advancing chip-scale Biosensing capabilities with optical microresonators. His laboratory has developed a microcavity Biosensing platform that is capable of monitoring single DNA molecules and their interaction kinetics, hence achieving an unprecedented sensitivity for label-free detection with light. By thermal stabilization of the sensor it is furthermore possible to characterize the structural change of biopolymers in response to temperature. Stand-off biodetection capabilities are demonstrated with asymmetric microspheres. Article not available.
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single molecule nucleic acid interactions monitored on a label free microcavity biosensor platform
Nature Nanotechnology, 2014Co-Authors: Martin D Baaske, Matthew R Foreman, Frank VollmerAbstract:Biosensing relies on the detection of molecules and their specific interactions. It is therefore highly desirable to develop transducers exhibiting ultimate detection limits. Microcavities are an exemplary candidate technology for demonstrating such a capability in the optical domain and in a label-free fashion. Additional sensitivity gains, achievable by exploiting plasmon resonances, promise Biosensing down to the single-molecule level. Here, we introduce a Biosensing platform using optical microcavity-based sensors that exhibits single-molecule sensitivity and is selective to specific single binding events. Whispering gallery modes in glass microspheres are used to leverage plasmonic enhancements in gold nanorods for the specific detection of nucleic acid hybridization, down to single 8-mer oligonucleotides. Detection of single intercalating small molecules confirms the observation of single-molecule hybridization. Matched and mismatched strands are discriminated by their interaction kinetics. Our platform allows us to monitor specific molecular interactions transiently, hence mitigating the need for high binding affinity and avoiding permanent binding of target molecules to the receptors. Sensor lifetime is therefore increased, allowing interaction kinetics to be statistically analysed.
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Microcavity Biosensing
Frontiers in Ultrafast Optics: Biomedical Scientific and Industrial Applications XI, 2011Co-Authors: Frank VollmerAbstract:A microcavity biosensor monitors optical resonances in micro-and nanostrucrtures for label-free detection of molecules and their interactions. I will give an introduction to the field of microcavity Biosensing and present an overview on the current state-of-the art. I will emphasize recent applications of optical microcavities for nanoparticle detection, trapping and manipulation, and I will highlight different modalities for ultra-sensitve label-free Biosensing
Arben Merkoçi - One of the best experts on this subject based on the ideXlab platform.
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Graphene oxide as an optical Biosensing platform
Advanced Materials, 2012Co-Authors: Eden Morales-narv??ez, Arben Merko??i, E. Morales-narváez, Arben MerkoçiAbstract:Since graphene exhibits innovative mechanical, electrical, thermal, and optical properties, this 2D material is increasingly attracting attention and is under active research. Among the various graphene forms with lattice-like nanostructure, graphene oxide (GO) displays advantageous characteristics as a Biosensing platform due to its excellent capabilities for direct wiring with biomolecules, a heterogeneous chemical and electronic structure, the possibility to be processed in solution and the ability to be tuned as insulator, semiconductor or semi-metal. Moreover, GO photoluminescences with energy transfer donor/acceptor molecules exposed in a planar surface and is even proposed as a universal highly efficient long-range quencher, which is opening the way to several unprecedented Biosensing strategies. Here, the rationale behind the use of GO in optical Biosensing applications is discussed by describing different potentially exploitable properties of GO, and an overview of the current approaches are presented along with future perspectives and challenges.
Xianqiang Mi - One of the best experts on this subject based on the ideXlab platform.
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Fluorescent biosensors enabled by graphene and graphene oxide
Biosensors and Bioelectronics, 2017Co-Authors: Huan Zhang, Honglu Zhang, Xiaolei Zuo, Chunhai Fan, Ali Aldalbahi, Xianqiang MiAbstract:During the past few years, graphene and graphene oxide (GO) have attracted numerous attentions for the potential applications in various fields from energy technology, Biosensing to biomedical diagnosis and therapy due to their various functionalization, high volume surface ratio, unique physical and electrical properties. Among which, graphene and graphene oxide based fluorescent biosensors enabled by their fluorescence-quenching properties have attracted great interests. The fluorescence of fluorophore or dye labeled on probes (such as molecular beacon, aptamer, DNAzymes and so on) was quenched after adsorbed on to the surface of graphene. While in the present of the targets, due to the strong interactions between probes and targets, the probes were detached from the surface of graphene, generating dramatic fluorescence, which could be used as signals for detection of the targets. This strategy was simple and economy, together with great programmable abilities of probes; we could realize detection of different kinds of species. In this review, we first briefly introduced the history of graphene and graphene oxide, and then summarized the fluorescent biosensors enabled by graphene and GO, with a detailed account of the design mechanism and comparison with other nanomaterials (e.g. carbon nanotubes and gold nanoparticles). Following that, different sensing platforms for detection of DNAs, ions, biomolecules and pathogens or cells as well as the cytotoxicity issue of graphene and GO based in vivo Biosensing were further discussed. We hope that this review would do some help to researchers who are interested in graphene related biosening research work.
