The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Raja Ghosh - One of the best experts on this subject based on the ideXlab platform.
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environment responsive hydrogel based ultrafiltration membranes for protein Bioseparation
Journal of Membrane Science, 2009Co-Authors: Ruixiang Huang, L K Kostanski, Carlos D M Filipe, Raja GhoshAbstract:Environment-responsive ultrafiltration membranes were created by modification of a commercial microporous membrane with a hydrogel composed of poly-N-vinyllactams cross-linked with bisacrylamide. The modified membranes were then characterized by their percentage mass gains as well as by their valve effect in response to change in salt concentration in the feed solution. The transmission behavior of model proteins through these membranes was studied using a parameter scanning ultrafiltration technique. A set of membranes selected for highest retention of intermediately sized proteins were examined for protein Bioseparation applications. The parameter scanning ultrafiltration technique was then used to examine the feasibility of separating a binary model protein mixture of equine ferritin and human immunoglobulin G by sequential transmission through the environment-responsive membrane. The results obtained demonstrated the potential for using these membranes for multi-component protein separation.
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purification of human igg using membrane based hybrid Bioseparation technique and its variants a comparative study
Separation and Purification Technology, 2009Co-Authors: Lu Wang, Kor Zheng Mah, Raja GhoshAbstract:Abstract This paper compares three different techniques for IgG purification from human serum, all of which are based on a recently developed membrane based hybrid Bioseparation strategy, i.e. combination of salt induced precipitation, microfiltration and membrane adsorption as one unit operation [R. Ghosh, Separation of human albumin and IgG by a membrane-based integrated Bioseparation technique involving simultaneous precipitation, microfiltration and membrane adsorption, J. Membr. Sci. 237 (2004) 109–117]. When used on its own, the standard hybrid Bioseparation technique gave 93% recovery of IgG from human serum with 85% purity. In order to obtain higher IgG purity, caprylic acid based precipitation was investigated as a second purification step. As an alternative approach to this, caprylic acid precipitation was integrated with the standard hybrid Bioseparation technique, resulting in a single step process. The antibody obtained using the three above mentioned techniques were then diafiltered to remove salts and caprylic acid and the overall purity and recovery were compared. The integrated hybrid–caprylic acid purification technique gave the highest IgG purity (95%) with the recovery being 55% while the purity and recovery using the two-step approach were 93% and 73% respectively. The trade off between purity, recovery and membrane fouling are discussed.
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purification of equine igg using membrane based enhanced hybrid Bioseparation technique a potential method for manufacturing hyperimmune antibody
Biotechnology and Bioengineering, 2008Co-Authors: Lu Wang, Raja GhoshAbstract:Hyperimmune equine IgG is widely used as antivenom and anti-rabies agents. This article discusses a membrane based enhanced hybrid Bioseparation technique for efficient and scalable purification of equine immunoglobulin G (IgG) from horse serum. This technique is an improved version of a standard hybrid Bioseparation technique developed within our group earlier for fractionation of human plasma proteins (Ghosh. 2004. J Membr Sci 237: 109–117). In the presence of a high antichaotropic salt concentration, equine IgG is selectively and reversibly captured within a stirred cell membrane module from horse serum, partly due to precipitation and microfiltration, and partly due to hydrophobic interaction based membrane adsorption, while the impurities are washed out from the device. The reversibly sequestered IgG is then released by lowering the salt concentration which favor both dissolution of the precipitated IgG and desorption of the membrane bound IgG. The enhanced hybrid Bioseparation technique improves the IgG recovery from the membrane module by switching from a stirring to non-stirring mode during the IgG release phase. It also reduces membrane fouling by an appropriate pH switch. The effects of operating conditions on equine IgG capture were first systematically studied. The enhanced hybrid Bioseparation technique was followed by an ultrafiltration step to remove ammonium sulfate and low molecular weight impurities. The equine IgG purity obtained under optimized conditions was 88% and its recovery was over 90%, both being significantly higher than corresponding values obtained using currently used purification techniques. Biotechnol. Bioeng. 2008;99: 625–633. © 2007 Wiley Periodicals, Inc.
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purification of equine igg using membrane based enhanced hybrid Bioseparation technique a potential method for manufacturing hyperimmune antibody
Biotechnology and Bioengineering, 2008Co-Authors: Lu Wang, Xinghua Sun, Raja GhoshAbstract:Hyperimmune equine IgG is widely used as antivenom and anti-rabies agents. This article discusses a membrane based enhanced hybrid Bioseparation technique for efficient and scalable purification of equine immunoglobulin G (IgG) from horse serum. This technique is an improved version of a standard hybrid Bioseparation technique developed within our group earlier for fractionation of human plasma proteins (Ghosh. 2004. J Membr Sci 237: 109-117). In the presence of a high antichaotropic salt concentration, equine IgG is selectively and reversibly captured within a stirred cell membrane module from horse serum, partly due to precipitation and microfiltration, and partly due to hydrophobic interaction based membrane adsorption, while the impurities are washed out from the device. The reversibly sequestered IgG is then released by lowering the salt concentration which favor both dissolution of the precipitated IgG and desorption of the membrane bound IgG. The enhanced hybrid Bioseparation technique improves the IgG recovery from the membrane module by switching from a stirring to non-stirring mode during the IgG release phase. It also reduces membrane fouling by an appropriate pH switch. The effects of operating conditions on equine IgG capture were first systematically studied. The enhanced hybrid Bioseparation technique was followed by an ultrafiltration step to remove ammonium sulfate and low molecular weight impurities. The equine IgG purity obtained under optimized conditions was 88% and its recovery was over 90%, both being significantly higher than corresponding values obtained using currently used purification techniques.
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purification of humanized monoclonal antibodies by membrane based hybrid Bioseparation technique
Journal of Immunological Methods, 2006Co-Authors: Lu Wang, Dharmesh M Kanani, Raja GhoshAbstract:We describe a rapid, inexpensive and scalable hybrid Bioseparation technique for one-step purification of humanized monoclonal antibodies from mammalian cell culture supernatant. It involves the selective and reversible retention of the monoclonal antibody within a membrane module utilizing a combination of microfiltration of precipitated antibody and simultaneous hydrophobic interaction-based membrane chromatography of soluble antibody on the same microfiltration membrane. The retained monoclonal antibody is subsequently recovered from the membrane module in a highly pure form by changing the solution condition to that which favours simultaneous antibody dissolution and elution from the membrane. This combination of separation principles results in high-capacity, high-resolution separation. The monoclonal antibody purities and recoveries obtained in the experiments described in this paper were very high.
Abul Hossain Molla - One of the best experts on this subject based on the ideXlab platform.
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mycoremediation a prospective environmental friendly technique of Bioseparation and dewatering of domestic wastewater sludge
Environmental Science and Pollution Research, 2012Co-Authors: Abul Hossain Molla, A FakhrulraziAbstract:Environmental safe and friendly management and disposal of wastewater sludge is a problem of every treatment plant throughout the world. Bioseparation and dewaterability of raw domestic wastewater sludge were evaluated for proper management and disposal by mycoremediation, i.e., using prior grown 2% (v/v) spore suspension of filamentous fungal (Mucor hiemalis Wehmer) broth inoculation, which were grown in 2% (w/v) solution of malt extract and wheat flour for 48–60 h in orbital shaker. Within 2–3 days of treatment application, encouraging results were achieved in total dry solids (TDS), total suspended solid (TSS), turbidity, chemical oxygen demand (COD), specific resistance to filtration (SRF), and pH due to fungal treatment in recognition of Bioseparation and dewaterability of wastewater sludge compared to control. The significant reduction of TDS was remarked at fungal biomass (FB) in wheat flour (WF) treatment. The removal of TSS, turbidity, COD, and SRF were observed 96.0%, 99.4%, 92.6%, and 97.6%, respectively, in supernatant at 5 days by FB in WF. The SRF measuring the dewaterability was decreased with maximum (0.26 × 10−12 mg/kg) equivalent to 95.5% at 2 days in FB in WF also. FB in WF broth is a potential, environmental friendly, comparatively low-cost biological technique which might play the significant role for bioremediation and Bioseparation of domestic wastewater sludge. The present technique may bring a dynamic change in treatment of wastewater in future.
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enhancement of Bioseparation and dewaterability of domestic wastewater sludge by fungal treated dewatered sludge
Journal of Hazardous Materials, 2007Co-Authors: A Fakhrulrazi, Abul Hossain MollaAbstract:Abstract A promising biological, sustainable, non-hazardous, safe and environmental friendly management and disposal technique of domestic wastewater sludge is global expectation. Fungal entrapped biosolids as a result of prior fungal treated raw wastewater sludge was recycled to evaluate its performance as inoculum for Bioseparation/bioconversion of supplemented sludge in view of continuous as well as scale up wastewater sludge treatment. Encouraging results were achieved in Bioseparation of suspended solids and in dewaterability/filterability of treated domestic wastewater sludge. Fungal entrapped biosolids offered 98% removal of total suspended solids (TSS) in supplemented sludge treatment at 6-day without nutrient (wheat flour, WF) supply. Consequently, 99% removal of turbidity and 87% removal of chemical oxygen demand (COD) were achieved in supernatant of treated sludge. The lowest value (1.75 × 1012 m/kg) of specific resistance to filtration (SRF) was observed at 6-day after treatment, which was equivalent to the 70% decrease of SRF. The all results except SRF were not influenced further in treatments accompanied with WF supplementation. The present treatments offered significant (P ≤ 0.01) improvement in all results except SRF of treated wastewater sludge compared to the control. Furthermore, the present result is addressing a potential avenue of probable solution for expected management and disposal of domestic wastewater sludge in future.
Lu Wang - One of the best experts on this subject based on the ideXlab platform.
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purification of human igg using membrane based hybrid Bioseparation technique and its variants a comparative study
Separation and Purification Technology, 2009Co-Authors: Lu Wang, Kor Zheng Mah, Raja GhoshAbstract:Abstract This paper compares three different techniques for IgG purification from human serum, all of which are based on a recently developed membrane based hybrid Bioseparation strategy, i.e. combination of salt induced precipitation, microfiltration and membrane adsorption as one unit operation [R. Ghosh, Separation of human albumin and IgG by a membrane-based integrated Bioseparation technique involving simultaneous precipitation, microfiltration and membrane adsorption, J. Membr. Sci. 237 (2004) 109–117]. When used on its own, the standard hybrid Bioseparation technique gave 93% recovery of IgG from human serum with 85% purity. In order to obtain higher IgG purity, caprylic acid based precipitation was investigated as a second purification step. As an alternative approach to this, caprylic acid precipitation was integrated with the standard hybrid Bioseparation technique, resulting in a single step process. The antibody obtained using the three above mentioned techniques were then diafiltered to remove salts and caprylic acid and the overall purity and recovery were compared. The integrated hybrid–caprylic acid purification technique gave the highest IgG purity (95%) with the recovery being 55% while the purity and recovery using the two-step approach were 93% and 73% respectively. The trade off between purity, recovery and membrane fouling are discussed.
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purification of equine igg using membrane based enhanced hybrid Bioseparation technique a potential method for manufacturing hyperimmune antibody
Biotechnology and Bioengineering, 2008Co-Authors: Lu Wang, Raja GhoshAbstract:Hyperimmune equine IgG is widely used as antivenom and anti-rabies agents. This article discusses a membrane based enhanced hybrid Bioseparation technique for efficient and scalable purification of equine immunoglobulin G (IgG) from horse serum. This technique is an improved version of a standard hybrid Bioseparation technique developed within our group earlier for fractionation of human plasma proteins (Ghosh. 2004. J Membr Sci 237: 109–117). In the presence of a high antichaotropic salt concentration, equine IgG is selectively and reversibly captured within a stirred cell membrane module from horse serum, partly due to precipitation and microfiltration, and partly due to hydrophobic interaction based membrane adsorption, while the impurities are washed out from the device. The reversibly sequestered IgG is then released by lowering the salt concentration which favor both dissolution of the precipitated IgG and desorption of the membrane bound IgG. The enhanced hybrid Bioseparation technique improves the IgG recovery from the membrane module by switching from a stirring to non-stirring mode during the IgG release phase. It also reduces membrane fouling by an appropriate pH switch. The effects of operating conditions on equine IgG capture were first systematically studied. The enhanced hybrid Bioseparation technique was followed by an ultrafiltration step to remove ammonium sulfate and low molecular weight impurities. The equine IgG purity obtained under optimized conditions was 88% and its recovery was over 90%, both being significantly higher than corresponding values obtained using currently used purification techniques. Biotechnol. Bioeng. 2008;99: 625–633. © 2007 Wiley Periodicals, Inc.
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purification of equine igg using membrane based enhanced hybrid Bioseparation technique a potential method for manufacturing hyperimmune antibody
Biotechnology and Bioengineering, 2008Co-Authors: Lu Wang, Xinghua Sun, Raja GhoshAbstract:Hyperimmune equine IgG is widely used as antivenom and anti-rabies agents. This article discusses a membrane based enhanced hybrid Bioseparation technique for efficient and scalable purification of equine immunoglobulin G (IgG) from horse serum. This technique is an improved version of a standard hybrid Bioseparation technique developed within our group earlier for fractionation of human plasma proteins (Ghosh. 2004. J Membr Sci 237: 109-117). In the presence of a high antichaotropic salt concentration, equine IgG is selectively and reversibly captured within a stirred cell membrane module from horse serum, partly due to precipitation and microfiltration, and partly due to hydrophobic interaction based membrane adsorption, while the impurities are washed out from the device. The reversibly sequestered IgG is then released by lowering the salt concentration which favor both dissolution of the precipitated IgG and desorption of the membrane bound IgG. The enhanced hybrid Bioseparation technique improves the IgG recovery from the membrane module by switching from a stirring to non-stirring mode during the IgG release phase. It also reduces membrane fouling by an appropriate pH switch. The effects of operating conditions on equine IgG capture were first systematically studied. The enhanced hybrid Bioseparation technique was followed by an ultrafiltration step to remove ammonium sulfate and low molecular weight impurities. The equine IgG purity obtained under optimized conditions was 88% and its recovery was over 90%, both being significantly higher than corresponding values obtained using currently used purification techniques.
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purification of humanized monoclonal antibodies by membrane based hybrid Bioseparation technique
Journal of Immunological Methods, 2006Co-Authors: Lu Wang, Dharmesh M Kanani, Raja GhoshAbstract:We describe a rapid, inexpensive and scalable hybrid Bioseparation technique for one-step purification of humanized monoclonal antibodies from mammalian cell culture supernatant. It involves the selective and reversible retention of the monoclonal antibody within a membrane module utilizing a combination of microfiltration of precipitated antibody and simultaneous hydrophobic interaction-based membrane chromatography of soluble antibody on the same microfiltration membrane. The retained monoclonal antibody is subsequently recovered from the membrane module in a highly pure form by changing the solution condition to that which favours simultaneous antibody dissolution and elution from the membrane. This combination of separation principles results in high-capacity, high-resolution separation. The monoclonal antibody purities and recoveries obtained in the experiments described in this paper were very high.
Pau Loke Show - One of the best experts on this subject based on the ideXlab platform.
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liquid biphasic system a recent Bioseparation technology
Processes, 2020Co-Authors: Kuan Shiong Khoo, Hui Yi Leong, Pau Loke Show, Kit Wayne Chew, Jun Wei Lim, Tau Chuan Ling, Hongwei YenAbstract:A well-known Bioseparation technique namely liquid biphasic system (LBS) has attracted many researchers’ interest for being an alternative Bioseparation technology for various kinds of biomolecules. The present review begins with an in-depth discussion on the fundamental principle of LBS and this is followed by the discussion on further development of various phase-forming components in LBS. Additionally, the implementation of various advance technologies to the LBS that is beneficial towards the efficiency of LBS for the extraction, separation, and purification of biomolecules was discussed. The key parameters affecting the LBS were presented and evaluated. Moreover, future prospect and challenges were highlighted to be a useful guide for future development of LBS. The efforts presented in this review will provide an insight for future researches in liquid-liquid separation techniques.
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liquid biphasic electric partitioning system as a novel integration process for betacyanins extraction from red purple pitaya and antioxidant properties assessment
Frontiers in Chemistry, 2019Co-Authors: Hui Yi Leong, Chien Wei Ooi, Chung Lim Law, Advina Lizah Julkifle, Yukaung Chang, Pau Loke ShowAbstract:Nowadays, downstream bioprocessing industries inclines towards the development of a green and high efficient Bioseparation technology. Betacyanins are presently gaining higher interest in the food science as driven by their high tinctorial strength and health promoting functional properties. In this study, a novel green integration process of liquid biphasic electric partitioning system (LBEPS) was proposed for betacyanins extraction from peel and flesh of red-purple pitaya. Initially, the betacyanins extraction using LBEPS with initial settings was compared with that of liquid biphasic partitioning system (LBPS), and the results revealed that both systems demonstrated a comparable betacyanins extraction. This was followed by further optimizing the LBEPS for better betacyanins extraction. Several operating parameters including operation time, voltage applied, and position of graphitic electrodes in the system were investigated. Moreover, comparison between optimized LBEPS and LBPS with optimized conditions of electric system (as post-treatment) as well as color characterization and antioxidant properties assessment were conducted. Overall, the betacyanins extraction employing the optimized LBEPS showed the significant highest values of betacyanins concentration in alcohol-rich top phase (C t ) and partition coefficient (K) of betacyanins from peel (99.256 ± 0.014% and 133.433 ± 2.566) and flesh (97.189 ± 0.172% and 34.665 ± 2.253) of red-purple pitaya. These results inferred that an optimal betacyanins extraction was successfully achieved by this approach. Also, the LBEPS with the peel and flesh showed phase volume ratio (V r ) values of 1.667 and 2.167, respectively, and this indicated that they have a clear biphasic separation. In addition, the peel and flesh extract obtained from the optimized LBEPS demonstrated different variations of red color as well as their antioxidant properties were well-retained. This article introduces a new, reliable, and effective Bioseparation approach for the extraction of biomolecules, which is definitely worth to explore further as a Bioseparation tool in the downstream bioprocessing.
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integration process for betacyanins extraction from peel and flesh of hylocereus polyrhizus using liquid biphasic electric flotation system and antioxidant activity evaluation
Separation and Purification Technology, 2019Co-Authors: Hui Yi Leong, Chien Wei Ooi, Chung Lim Law, Advina Lizah Julkifle, Tomohisa Katsuda, Pau Loke ShowAbstract:Abstract Recently, betacyanins have added research applications in food science processing industries, owing to their health promoting functional and attractive visual properties. Additionally, red-purple pitaya ( Hylocereus polyrhizus ) is a rich source of betacyanins. Development of a green and effective Bioseparation technology is deemed favourably in the downstream bioprocessing industries. Hence, this study utilised a liquid biphasic electric flotation (LBEF) system for betacyanins extraction from peel and flesh of red-purple pitaya as well as comparison between LBF (liquid biphasic flotation) and LBEF system, color characterisation and antioxidant activity evaluation were conducted. In this study, the optimised LBF system was integrated with electric system. Collectively, the betacyanins extraction using LBEF system showed an optimal betacyanins extraction from the peel and flesh, with the significant highest values of betacyanins concentration in alcohol-rich top phase (C t ) (99.014 ± 0.074% and 96.132 ± 0.154%), separation efficiency (E) (98.383 ± 0.215% and 96.576 ± 0.083%) and partition coefficient (K) (100.814 ± 7.324 and 24.883 ± 1.052) of betacyanins. Moreover, the peel and flesh extract showed different variations of red colors and antioxidant properties. The present study introduces a new, simple and high efficient Bioseparation technology (LBEF system) which is definitely worth to study further.
A Fakhrulrazi - One of the best experts on this subject based on the ideXlab platform.
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mycoremediation a prospective environmental friendly technique of Bioseparation and dewatering of domestic wastewater sludge
Environmental Science and Pollution Research, 2012Co-Authors: Abul Hossain Molla, A FakhrulraziAbstract:Environmental safe and friendly management and disposal of wastewater sludge is a problem of every treatment plant throughout the world. Bioseparation and dewaterability of raw domestic wastewater sludge were evaluated for proper management and disposal by mycoremediation, i.e., using prior grown 2% (v/v) spore suspension of filamentous fungal (Mucor hiemalis Wehmer) broth inoculation, which were grown in 2% (w/v) solution of malt extract and wheat flour for 48–60 h in orbital shaker. Within 2–3 days of treatment application, encouraging results were achieved in total dry solids (TDS), total suspended solid (TSS), turbidity, chemical oxygen demand (COD), specific resistance to filtration (SRF), and pH due to fungal treatment in recognition of Bioseparation and dewaterability of wastewater sludge compared to control. The significant reduction of TDS was remarked at fungal biomass (FB) in wheat flour (WF) treatment. The removal of TSS, turbidity, COD, and SRF were observed 96.0%, 99.4%, 92.6%, and 97.6%, respectively, in supernatant at 5 days by FB in WF. The SRF measuring the dewaterability was decreased with maximum (0.26 × 10−12 mg/kg) equivalent to 95.5% at 2 days in FB in WF also. FB in WF broth is a potential, environmental friendly, comparatively low-cost biological technique which might play the significant role for bioremediation and Bioseparation of domestic wastewater sludge. The present technique may bring a dynamic change in treatment of wastewater in future.
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enhancement of Bioseparation and dewaterability of domestic wastewater sludge by fungal treated dewatered sludge
Journal of Hazardous Materials, 2007Co-Authors: A Fakhrulrazi, Abul Hossain MollaAbstract:Abstract A promising biological, sustainable, non-hazardous, safe and environmental friendly management and disposal technique of domestic wastewater sludge is global expectation. Fungal entrapped biosolids as a result of prior fungal treated raw wastewater sludge was recycled to evaluate its performance as inoculum for Bioseparation/bioconversion of supplemented sludge in view of continuous as well as scale up wastewater sludge treatment. Encouraging results were achieved in Bioseparation of suspended solids and in dewaterability/filterability of treated domestic wastewater sludge. Fungal entrapped biosolids offered 98% removal of total suspended solids (TSS) in supplemented sludge treatment at 6-day without nutrient (wheat flour, WF) supply. Consequently, 99% removal of turbidity and 87% removal of chemical oxygen demand (COD) were achieved in supernatant of treated sludge. The lowest value (1.75 × 1012 m/kg) of specific resistance to filtration (SRF) was observed at 6-day after treatment, which was equivalent to the 70% decrease of SRF. The all results except SRF were not influenced further in treatments accompanied with WF supplementation. The present treatments offered significant (P ≤ 0.01) improvement in all results except SRF of treated wastewater sludge compared to the control. Furthermore, the present result is addressing a potential avenue of probable solution for expected management and disposal of domestic wastewater sludge in future.