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Coby Schal - One of the best experts on this subject based on the ideXlab platform.
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Intracellular Localization of Blattella germanica Densovirus (BgDV1) Capsid Proteins.
Viruses, 2018Co-Authors: E. N. Kozlov, Coby Schal, Elena Martynova, Vladimir I. Popenko, D. V. MukhaAbstract:Densovirus genome replication and capsid assembly take place in the nucleus of the infected cells. However, the mechanisms underlying such processes as the delivery of virus proteins to the nucleus and the export of progeny virus from the nucleus remain elusive. It is evident that nuclear transport signals should be involved in these processes. We performed an in silico search for the putative nuclear localization signal (NLS) and nuclear export signal (NES) motifs in the capsid proteins of the Blattella germanica Densovirus 1 (BgDV1) densovirus. A high probability NLS motif was found in the common C-terminal of capsid proteins together with a NES motif in the unique N-terminal of VP2. We also performed a global search for the nuclear traffic signals in the densoviruses belonging to five Densovirinae genera, which revealed high diversity in the patterns of NLSs and NESs. Using a heterologous system, the HeLa mammalian cell line expressing GFP-fused BgDV1 capsid proteins, we demonstrated that both signals are functionally active. We suggest that the NLS shared by all three BgDV1 capsid proteins drives the trafficking of the newly-synthesized proteins into the nucleus, while the NES may play a role in the export of the newly-assembled BgDV1 particles into the cytoplasm through nuclear pore complexes.
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laboratory and field evaluation of zyrox fly granular bait against asian and german cockroaches dictyoptera blattellidae
Journal of Economic Entomology, 2016Co-Authors: Yvonne K Matos, Coby SchalAbstract:The Asian cockroach (Blattella asahinai Mizukubo) was introduced to Florida in 1986 and has since spread throughout the Southeastern United States. Blattella asahinai is a peridomestic pest and high population densities in residential areas can become a nuisance, especially when adults fly into homes. Few studies to date have been conducted on Asian cockroach control, and we evaluated the efficacy of Zyrox Fly Granular Bait and Maxforce Complete Granular Insect Bait against this species in the laboratory compared with the closely related German cockroach (Blattella germanica (L.)). In no-choice and two-choice assays with both species, Zyrox bait and Maxforce bait achieved nearly 100% mortality within two and five days, respectively. We also tested Zyrox bait against B. asahinai in an invasive field population in North Carolina at the label rate (2 g/m(2)) and at approximately three times the label rate (6.9 g/m(2)), and found that broadcast applications at both rates reduced populations by an average of 64 and 92%, respectively, for 35 d after the initial application. Zyrox Fly Bait appears to be effective against the Asian and German cockroaches, and could be another tool in an integrated pest management program, if its label could be extended or the active ingredient (cyantraniliprole) formulated into a cockroach bait.
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Intracellular localization of regulatory proteins of the German cockroach Blattella germanica densovirus
Molecular Biology, 2014Co-Authors: E. U. Martynova, Coby Schal, T. V. Kapelinskaya, D. V. MukhaAbstract:The use of western blot analysis of nuclear and cytoplasmic extracts of BgDNV densovirusinfected German cockroach, Blattella germanica , the intracellular localization of the regulatory proteins of the corresponding densovirus was investigated in cell culture. It was demonstrated that two proteins, namely NS1 and NS3, were predominantly localized in the nucleus, whereas NS2 protein was equally distributed in the nuclei and the cytoplasm. The data obtained are important for understanding the potential functions of densovirus regulatory proteins. The intracellular localization of NS3 protein was determined for the first time for any densovirus.
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Social interaction facilitates reproduction in male German cockroaches, Blattella germanica
Animal Behaviour, 2013Co-Authors: Adrienn Uzsák, Coby SchalAbstract:Sociality can be beneficial for individuals because it may increase access to resources, provide greater protection from predators and create opportunities for mating. In some species, social conditions and experience also influence the physiology and behavioural responses of individuals. Although social facilitation of sexual maturation is poorly understood in insects, the German cockroach has served as an important model system for this phenomenon as group-housed females mature faster than solitary females. However, social modulation of male reproduction has not been investigated in this or any other nonpolyphenic insect species. We investigated the relative effects of social interactions and isolation on endocrine, reproductive and behavioural events during sexual maturation of B. germanica males. Results show that social interactions significantly facilitate juvenile hormone biosynthesis as well as protein production by the accessory reproductive glands, whereas isolation suppresses these processes. Other processes, such as accumulation of cuticular hydrocarbons, maturation of the male's courtship readiness and his readiness to copulate, appear to be relatively independent of social experience. However, both courtship and copulation latencies declined with age more in socially paired males than in isolated males. Our study is the first to reveal that social interactions have a profound effect on certain aspects of male reproductive physiology and behaviour in an insect species that does not exhibit phase or developmental polyphenism.
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Blattella asahinai dictyoptera blattellidae a new predator of lepidopteran eggs in south texas soybean
Annals of The Entomological Society of America, 2008Co-Authors: Robert S Pfannenstiel, Edward L Vargo, Warren Booth, Coby SchalAbstract:Abstract Large numbers of a cockroach that resembled the German cockroach, Blattella germanica (L.), were observed during winter 2005–2006 in home turf settings in Weslaco, TX, 11 km from the U.S. border with northeastern Mexico. In June and July 2006, this cockroach was observed at night on the foliage of soybean, Glycine max (L.) Merr., in numbers often exceeding 50 per m row. Because of its similarity to B. germanica, but with the characteristic of flying frequently, we suspected that our observations might be of the Asian cockroach, Blattella asahinai Mizukubo. Using a combination of morphological characters, cuticular hydrocarbons, and sequences of the mitochondrial cytochrome oxidase II gene, we definitively identified this cockroach as B. asahinai. B. asahinai was frequently observed feeding on sentinel eggs of Helicoverpa zea (Boddie) and Spodoptera exigua (Hubner). This cockroach was the dominant egg predator in soybean during 2006, making up 36.4% of all predators observed feeding on eggs. B. as...
Jose L Maestro - One of the best experts on this subject based on the ideXlab platform.
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sirna enrichment in argonaute 2 depleted Blattella germanica
Biochimica et Biophysica Acta, 2021Co-Authors: Jose Carlos Montanes, Maria-dolors Piulachs, Guillem Ylla, Carlos Rojano, Jose L MaestroAbstract:Abstract Background RNA interference (RNAi) is a cellular mechanism used to fight various threats, including transposons, aberrant RNAs, and some types of viruses. This mechanism relies on the detection of dsRNA molecules, which through a pathway involving Dicer-2 (Dcr-2) and Argonaute 2 (AGO2), produces small interfering RNAs (siRNAs) that bind to the complementary RNAs triggering their degradation. Methods Using the cockroach Blattella germanica as a model, we examined AGO2 activity by depleting its mRNA using RNAi and analyzing the phenotypes produced. Results Depleting AGO2 expression had no remarkable effect on nymphal development or reproduction. dsRNA treatment triggered an immediate and transitory increase in AGO2 expression, independently of Dcr-2 action. In addition, we analyzed the siRNAs generated after injecting a heterologous dsRNA in control and AGO2 -depleted animals. The results revealed that obtained siRNAs mapped non-uniformly along the dsRNA sequence . In AGO2 -depleted animals, the proportion of 22 nucleotide reads was higher and accumulations of reads appeared in areas less well-represented in the controls. We also detected a preference for cytosine as the first nucleotide in controls that was significantly attenuated in AGO2-depleted individuals. Conclusions/general significance The siRNAs produced from a dsRNA mapped heterogeneously along the length of the dsRNA and this arrangement depends on the dsRNA sequence. AGO2 exerts its role as nuclease on the siRNA duplexes independently of its action on the corresponding mRNA. This study sheds light on an extremely useful process for reverse genetics in laboratories, in addition to the design of more effective, specific, and eco-friendly pest-control strategies.
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sirna enrichment in argonaute 2 depleted Blattella germanica
bioRxiv, 2021Co-Authors: Jose Carlos Montanes, Maria-dolors Piulachs, Guillem Ylla, Carlos Rojano, Jose L MaestroAbstract:BackgroundRNA interference (RNAi) is a cellular mechanism used to fight various threats, including transposons, aberrant RNA, and some types of viruses. It relies on dsRNA detection and, through a mechanism involving Dicer-2 (Dcr-2) and Argonaute 2 (AGO2), together with a small RNA fragment (siRNA) as a complementary guide, binds to an RNA and cleaves it. MethodsUsing the cockroach Blattella germanica as a model, we examined AGO2 activity by depleting its mRNA levels using RNAi and analyzing the phenotypes produced. ResultsSilencing AGO2 expression had no remarkable effect on nymphal development or reproduction. dsRNA treatment triggered an immediate and transitory increase in AGO2 expression, independently of Dcr-2 action. In addition, we analyzed the siRNAs generated after injecting a heterologous dsRNA in control and AGO2-depleted animals. The results revealed that siRNAs were distributed non-uniformly along the dsRNA sequence. In AGO2-depleted animals, the proportion of 22 nucleotide reads was higher and accumulations of reads appeared in areas less well-represented in the controls. We also detected a slight preference for cytosine as the first nucleotide in controls to be lost in AGO2-depleted individuals. Conclusions/General significanceThe siRNAs produced from a dsRNA are heterogeneously distributed along the length of the dsRNA and this arrangement depends on the sequence. AGO2 exerts its role as nuclease on the siRNA duplexes independently of its action on the homologous mRNA. This study sheds light on an extremely useful process for reverse genetics in laboratories, in addition to the design of more effective, specific, and eco-friendly pest-control strategies. Highlights- RNA interference is used to fight cellular threats including transposons and viruses - Silencing AGO2 in Blattella germanica does not affect development or reproduction - siRNA distribution along the dsRNA sequence is non-uniform and sequence dependent - AGO2 depletion increased the proportion of 22 nt reads in certain areas
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leucomyosuppressin modulates cardiac rhythm in the cockroach Blattella germanica
Journal of Insect Physiology, 2011Co-Authors: Jose L Maestro, Stephen S Tobe, Xavier BellesAbstract:Several lines of evidence point to leucomyosuppressin (LMS) and myosuppressin-related peptides as inhibitory modulators of heartbeat frequency in arthropods. Previous studies in Blattella germanica demonstrated that heartbeat frequency decreases after ootheca formation, and remains low during the period of ootheca transport. Subsequent work in this cockroach resulted in the characterization of LMS and the cloning and sequencing of its precursor. The present paper describes the activity of LMS on modulation of heartbeat in B. germanica. Assays using semi-isolated heart preparations revealed that LMS reduces heartbeat frequency in a dose dependent manner, at physiological concentrations. Additional experiments showed that LMS inhibits heartbeat rates in vivo. Finally, injection of dsRNA for LMS elicited a decrease in LMS mRNA to virtually undetectable levels and heartbeat frequency increased significantly in females carrying oothecae. These data suggest that LMS contributes to the modulation of cardiac rhythm in B. germanica during the reproductive cycle.
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juvenile hormone production and accessory reproductive gland development during sexual maturation of male Blattella germanica l dictyoptera blattellidae
Comparative Biochemistry and Physiology Part A: Physiology, 1992Co-Authors: Maria-dolors Piulachs, Jose L Maestro, Xavier BellesAbstract:Abstract 1. 1. A study of the release of juvenile hormone (JH) by in vitro -incubated corpora allata (CA) and on the development of the accessory reproductive glands (ARG) during sexual maturation of male Blattella germanica has been performed. 2. 2. JH release rates are comparable with those observed in previtellogenic females. 3. 3. The ARG undergo a progressive increase in size and protein content during sexual maturation. 4. 4. After CA removal, ARG protein accumulation is reduced (by 50%) in comparison with controls, and administration of exogenous JH restores normal development. 5. 5. The involvement of JH in ARG growth is discussed.
Xavier Belles - One of the best experts on this subject based on the ideXlab platform.
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Additional file 3: Table S3. of Comparative analysis of miRNA expression during the development of insects of different metamorphosis modes and germ-band types
2017Co-Authors: Guillem Ylla, Maria-dolors Piulachs, Xavier BellesAbstract:Read counts of each miRNA gene in the Blattella germanica stage-libraries studied. (XLS 56Â kb
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leucomyosuppressin modulates cardiac rhythm in the cockroach Blattella germanica
Journal of Insect Physiology, 2011Co-Authors: Jose L Maestro, Stephen S Tobe, Xavier BellesAbstract:Several lines of evidence point to leucomyosuppressin (LMS) and myosuppressin-related peptides as inhibitory modulators of heartbeat frequency in arthropods. Previous studies in Blattella germanica demonstrated that heartbeat frequency decreases after ootheca formation, and remains low during the period of ootheca transport. Subsequent work in this cockroach resulted in the characterization of LMS and the cloning and sequencing of its precursor. The present paper describes the activity of LMS on modulation of heartbeat in B. germanica. Assays using semi-isolated heart preparations revealed that LMS reduces heartbeat frequency in a dose dependent manner, at physiological concentrations. Additional experiments showed that LMS inhibits heartbeat rates in vivo. Finally, injection of dsRNA for LMS elicited a decrease in LMS mRNA to virtually undetectable levels and heartbeat frequency increased significantly in females carrying oothecae. These data suggest that LMS contributes to the modulation of cardiac rhythm in B. germanica during the reproductive cycle.
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deep sequencing of organ and stage specific micrornas in the evolutionarily basal insect Blattella germanica l dictyoptera blattellidae
PLOS ONE, 2011Co-Authors: Alexandre S Cristino, Maria-dolors Piulachs, Erica D Tanaka, Mercedes Rubio, Xavier BellesAbstract:Background: microRNAs (miRNAs) have been reported as key regulators at post-transcriptional level in eukaryotic cells. In insects, most of the studies have focused in holometabolans while only recently two hemimetabolans (Locusta migratoria and Acyrthosiphon pisum) have had their miRNAs identified. Therefore, the study of the miRNAs of the evolutionarily basal hemimetabolan Blattella germanica may provide valuable insights on the structural and functional evolution of miRNAs.
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allatostatic neuropeptides from the cockroach Blattella germanica l dictyoptera blattellidae identification immunolocalization and activity
Regulatory Peptides, 1994Co-Authors: Xavier Belles, Maria-dolors Piulachs, Joseluis Maestro, Anders H Johnsen, Hanne Duve, Alan ThorpeAbstract:Four allatostatic neuropeptides were isolated from extracts of the brain of the cockroach Blattella germanica. The primary structures of these peptides were assigned as Leu-Tyr-Asp-Phe-Gly-Leu-NH2 (BLAST-1), Asp-Arg-Leu-Tyr-Ser-Phe-Gly-Leu-NH2 (BLAST-2), Ala-Gly-Ser-Asp-Gly-Arg-Leu-Tyr-Ser-Phe-Gly-Leu-NH2 (BLAST-3) and Ala-Pro-Ser-Ser-Ala-Gln-Arg-Leu-Tyr-Gly-Phe-Gly-Leu-NH2 (BLAST-4). Each of the peptides showed C-terminal amino acid sequence similarity to cockroach allatostatins and blowfly callatostatins. The four peptides inhibited in vitro juvenile hormone production by corpora allata from virgin females of B. germanica. Immunoreactivity against allatostatins was seen in the lateral neurosecretory neurons and in the axonal pathway leading to the corpora allata.
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induction of choriogenesis by 20 hydroxyecdysone in the german cockroach
Tissue & Cell, 1993Co-Authors: Xavier Belles, Nuria Pascual, Y Rosso, Xim Cerdá, Philippe Cassier, M. André, Maria-dolors PiulachsAbstract:Abstract Experiments in vitro have shown that 20-hydroxyecdysone (at a concentration of 0.2 and 2 μM and after 12 and 24 hr of incubation) is able to induce the precocious deposition of chorion materials by the follicular epithelium of young oocytes of the cockroach Blattella germanica. Since previous studies had shown that 20-hydroxyecdysone levels in B. germanica ovaries increase with oocyte maturation to reach a peak just before oviposition, we therefore hypothesize that ovarian ecdysteroids trigger Choriogenesis in this species through an autocrine action.
Maria-dolors Piulachs - One of the best experts on this subject based on the ideXlab platform.
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sirna enrichment in argonaute 2 depleted Blattella germanica
Biochimica et Biophysica Acta, 2021Co-Authors: Jose Carlos Montanes, Maria-dolors Piulachs, Guillem Ylla, Carlos Rojano, Jose L MaestroAbstract:Abstract Background RNA interference (RNAi) is a cellular mechanism used to fight various threats, including transposons, aberrant RNAs, and some types of viruses. This mechanism relies on the detection of dsRNA molecules, which through a pathway involving Dicer-2 (Dcr-2) and Argonaute 2 (AGO2), produces small interfering RNAs (siRNAs) that bind to the complementary RNAs triggering their degradation. Methods Using the cockroach Blattella germanica as a model, we examined AGO2 activity by depleting its mRNA using RNAi and analyzing the phenotypes produced. Results Depleting AGO2 expression had no remarkable effect on nymphal development or reproduction. dsRNA treatment triggered an immediate and transitory increase in AGO2 expression, independently of Dcr-2 action. In addition, we analyzed the siRNAs generated after injecting a heterologous dsRNA in control and AGO2 -depleted animals. The results revealed that obtained siRNAs mapped non-uniformly along the dsRNA sequence . In AGO2 -depleted animals, the proportion of 22 nucleotide reads was higher and accumulations of reads appeared in areas less well-represented in the controls. We also detected a preference for cytosine as the first nucleotide in controls that was significantly attenuated in AGO2-depleted individuals. Conclusions/general significance The siRNAs produced from a dsRNA mapped heterogeneously along the length of the dsRNA and this arrangement depends on the dsRNA sequence. AGO2 exerts its role as nuclease on the siRNA duplexes independently of its action on the corresponding mRNA. This study sheds light on an extremely useful process for reverse genetics in laboratories, in addition to the design of more effective, specific, and eco-friendly pest-control strategies.
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sirna enrichment in argonaute 2 depleted Blattella germanica
bioRxiv, 2021Co-Authors: Jose Carlos Montanes, Maria-dolors Piulachs, Guillem Ylla, Carlos Rojano, Jose L MaestroAbstract:BackgroundRNA interference (RNAi) is a cellular mechanism used to fight various threats, including transposons, aberrant RNA, and some types of viruses. It relies on dsRNA detection and, through a mechanism involving Dicer-2 (Dcr-2) and Argonaute 2 (AGO2), together with a small RNA fragment (siRNA) as a complementary guide, binds to an RNA and cleaves it. MethodsUsing the cockroach Blattella germanica as a model, we examined AGO2 activity by depleting its mRNA levels using RNAi and analyzing the phenotypes produced. ResultsSilencing AGO2 expression had no remarkable effect on nymphal development or reproduction. dsRNA treatment triggered an immediate and transitory increase in AGO2 expression, independently of Dcr-2 action. In addition, we analyzed the siRNAs generated after injecting a heterologous dsRNA in control and AGO2-depleted animals. The results revealed that siRNAs were distributed non-uniformly along the dsRNA sequence. In AGO2-depleted animals, the proportion of 22 nucleotide reads was higher and accumulations of reads appeared in areas less well-represented in the controls. We also detected a slight preference for cytosine as the first nucleotide in controls to be lost in AGO2-depleted individuals. Conclusions/General significanceThe siRNAs produced from a dsRNA are heterogeneously distributed along the length of the dsRNA and this arrangement depends on the sequence. AGO2 exerts its role as nuclease on the siRNA duplexes independently of its action on the homologous mRNA. This study sheds light on an extremely useful process for reverse genetics in laboratories, in addition to the design of more effective, specific, and eco-friendly pest-control strategies. Highlights- RNA interference is used to fight cellular threats including transposons and viruses - Silencing AGO2 in Blattella germanica does not affect development or reproduction - siRNA distribution along the dsRNA sequence is non-uniform and sequence dependent - AGO2 depletion increased the proportion of 22 nt reads in certain areas
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Additional file 3: Table S3. of Comparative analysis of miRNA expression during the development of insects of different metamorphosis modes and germ-band types
2017Co-Authors: Guillem Ylla, Maria-dolors Piulachs, Xavier BellesAbstract:Read counts of each miRNA gene in the Blattella germanica stage-libraries studied. (XLS 56Â kb
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deep sequencing of organ and stage specific micrornas in the evolutionarily basal insect Blattella germanica l dictyoptera blattellidae
PLOS ONE, 2011Co-Authors: Alexandre S Cristino, Maria-dolors Piulachs, Erica D Tanaka, Mercedes Rubio, Xavier BellesAbstract:Background: microRNAs (miRNAs) have been reported as key regulators at post-transcriptional level in eukaryotic cells. In insects, most of the studies have focused in holometabolans while only recently two hemimetabolans (Locusta migratoria and Acyrthosiphon pisum) have had their miRNAs identified. Therefore, the study of the miRNAs of the evolutionarily basal hemimetabolan Blattella germanica may provide valuable insights on the structural and functional evolution of miRNAs.
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allatostatic neuropeptides from the cockroach Blattella germanica l dictyoptera blattellidae identification immunolocalization and activity
Regulatory Peptides, 1994Co-Authors: Xavier Belles, Maria-dolors Piulachs, Joseluis Maestro, Anders H Johnsen, Hanne Duve, Alan ThorpeAbstract:Four allatostatic neuropeptides were isolated from extracts of the brain of the cockroach Blattella germanica. The primary structures of these peptides were assigned as Leu-Tyr-Asp-Phe-Gly-Leu-NH2 (BLAST-1), Asp-Arg-Leu-Tyr-Ser-Phe-Gly-Leu-NH2 (BLAST-2), Ala-Gly-Ser-Asp-Gly-Arg-Leu-Tyr-Ser-Phe-Gly-Leu-NH2 (BLAST-3) and Ala-Pro-Ser-Ser-Ala-Gln-Arg-Leu-Tyr-Gly-Phe-Gly-Leu-NH2 (BLAST-4). Each of the peptides showed C-terminal amino acid sequence similarity to cockroach allatostatins and blowfly callatostatins. The four peptides inhibited in vitro juvenile hormone production by corpora allata from virgin females of B. germanica. Immunoreactivity against allatostatins was seen in the lateral neurosecretory neurons and in the axonal pathway leading to the corpora allata.
Raul Adolfo Alzogaray - One of the best experts on this subject based on the ideXlab platform.
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lufenuron kills deltamethrin resistant Blattella germanica blattodea
Revista de la Sociedad Entomológica Argentina, 2018Co-Authors: Emilia Seccacini, Laura Wilma Juan, Claudia Vassena, Eduardo Zerba, Raul Adolfo AlzogarayAbstract:Lufenuron is a chitin synthesis inhibitor used for controlling several pest insects. The German cockroach, Blattella germanica , is a cosmopolitan insect of medical and economic importance. Resistance to insecticides has been worldwide reported in this species. The objective of this work was to evaluate the effect of lufenuron in two colonies of B. germanica , one susceptible to insecticides (CIPEIN) and another pyrethroid-resistant (JUBA). Experimental populations from each colony were exposed to filter papers treated with 50 mg of lufenuron/m2. Controls were exposed to filter papers treated with solvent alone. After seven weeks, the average size of both lufenuron-treated populations was 1.33 ± 0.7 individuals, while the average size of control populations were 206.5 ± 39.5 (CIPEIN) and 136.5 ± 43.5 (JUBA) individuals. This drastic effect suggests that lufenuron could be used as an alternative for the control of pyrethroid-resistant German cockroaches. Future studies should explore the toxicity of lufenuron on other populations of B. germanica resistant to pyrethroids or other insecticides
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Lufenuron kills deltamethrin-resistant Blattella germanica (Blattodea)
Sociedad Entomológica Argentina, 2018Co-Authors: Emilia Seccacini, Laura Wilma Juan, Claudia Vassena, Eduardo Zerba, Raul Adolfo AlzogarayAbstract:Lufenurón es un inhibidor de la síntesis de quitina usado para controlar diversas plagas de insectos. La cucaracha alemana, Blattella germanica, es un insecto cosmopolita de importancia médica y económica. Existen numerosos reportes de resistencia a insecticidas en poblaciones de B. germanica alrededor del mundo. El objetivo de este trabajo fue evaluar el efecto de lufenurón en dos colonias de B. germanica, una susceptible a insecticidas (CIPEIN) y otra resistente a deltametrina (JUBA). Se expusieron poblaciones experimentales de ambas colonias a papeles de filtro tratados con 50 mg de lufenurón/m2. Los controles fueron expuestos a papeles de filtro tratados con solvente solo. Al cabo de siete semanas, las dos poblaciones expuestas a lufenurón presentaron el mismo tamaño promedio (1,3 ± 0,7 individuos); mientras que los tamaños promedio de las poblaciones controles fueron 206,5 ± 39,5 (CIPEIN) y 136,5 ± 43,5 individuos (JUBA). Este drástico efecto sobre el tamaño poblacional sugiere que lufenurón podría ser usado como una alternativa para controlar cucarachas alemanas resistentes a deltametrina. A la vista de estos resultados, se debería explorar la toxicidad de lufenurón en otras poblaciones de B. germanica resistentes a piretroides u otros insecticidas
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locomotor behaviour of Blattella germanica modified by deet
PLOS ONE, 2013Co-Authors: Valeria Sfara, Eduardo Zerba, Gaston Mougaburecueto, Raul Adolfo AlzogarayAbstract:N,N-diethyl-3-methylbenzamide (DEET) is the active principle of most insect repellents used worldwide. However, its toxicity on insects has not been widely studied. The aim of this work is to study the effects of DEET on the locomotor activity of Blattella germanica. DEET has a dose-dependent repellent activity on B. germanica. Locomotor activity was significantly lower when insects were pre-exposed to 700 µg/cm2 of DEET for 20 or 30 minutes, but it did not change when pre-exposure was shorter. Locomotor activity of insects that were pre-exposed to 2.000 µg/cm2 of DEET for 10 minutes was significantly lower than the movement registered in controls. No differences were observed when insects were pre-exposed to lower concentrations of DEET. A 30-minute pre-exposure to 700 µg/cm2 of DEET caused a significant decrease in locomotor activity. Movement was totally recovered 24 h later. The locomotor activity measured during the exposure to different concentrations of DEET remained unchanged. Insects with decreased locomotor activity were repelled to the same extent than control insects by the same concentration of DEET. We demonstrated that the repellency and modification of locomotor activity elicited by DEET are non-associated phenomena. We also suggested that the reduction in locomotor activity indicates toxicity of DEET, probably to insect nervous system.
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Repellence and toxicity of Schinus molle extracts on Blattella germanica.
Fitoterapia, 2007Co-Authors: Adriana Alicia Ferrero, C. Sánchez Chopa, J.o. Werdin González, Raul Adolfo AlzogarayAbstract:The biological activities of ethanol and petroleum ether extracts from leaves and fruits of Schinus molle against adults of Blattella germanica were examined by repellence test and topical application. All extracts produced significant repellent effect and mortality.