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Blood Serum

The Experts below are selected from a list of 180 Experts worldwide ranked by ideXlab platform

Shaoyi Jiang – 1st expert on this subject based on the ideXlab platform

  • functionalizable and ultra stable nanoparticles coated with zwitterionic poly carboxybetaine in undiluted Blood Serum
    Biomaterials, 2009
    Co-Authors: Wei Yang, Lei Zhang, Shanlin Wang, Andrew D White, Shaoyi Jiang

    Abstract:

    A new surface chemistry presenting an abundance of functional groups for ligand immobilization in an ultra-low fouling background all in one material for nanoparticles was introduced. This surface platform, as demonstrated by zwitterionic poly(carboxybetaine acrylamide) (polyCBAA) coated nanoparticles, is not only ultra stable in undiluted human Blood Serum, but also can be conjugated to biomolecules conveniently and effectively. Thus, this surface chemistry is ideal to create multi-functional nanoparticles for targeted delivery and diagnostics. In addition, this work clearly shows that 10% Blood Serum commonly used to evaluate the stability of nanoparticles is insufficient and a new evaluation criterion with undiluted Blood Serum is recommended.

  • pursuing zero protein adsorption of poly carboxybetaine from undiluted Blood Serum and plasma
    Langmuir, 2009
    Co-Authors: Wei Yang, Wei Li, Jinli Zhang, Shaoyi Jiang

    Abstract:

    Human Blood Serum and plasma pose significant challenges to Blood-contacting devices and implanted materials because of their high nonspecific adsorption onto surfaces. In this work, we investigated nonspecific protein adsorption from single protein solutions and complex media such as undiluted human Blood Serum and plasma onto poly(carboxybetaine acrylamide) (polyCBAA)-grafted surfaces at different temperatures. The polyCBAA grafting was done via atom-transfer radical polymerization (ATRP) with varying film thicknesses. The objective is to create a surface that experiences “zero” protein adsorption from complex undiluted human Blood Serum and plasma. Results show that protein adsorption from undiluted human Blood Serum, plasma, and aged Serum on the polyCBAA-grafted surface is undetectable at both 25 and 37 °C by a surface plasmon resonance (SPR) sensor. This was achieved with a film thickness of ∼21 nm. Furthermore, it is demonstrated that the polyCBAA surfaces after antibody immobilization maintain und…

  • film thickness dependence of protein adsorption from Blood Serum and plasma onto poly sulfobetaine grafted surfaces
    Langmuir, 2008
    Co-Authors: Wei Yang, Wei Li, Jinli Zhang, Shengfu Chen, Gang Cheng, Hana Vaisocherova, Shaoyi Jiang

    Abstract:

    In this work, we investigate protein adsorption from single protein solutions and complex media such as 100% Blood Serum and plasma onto poly(sulfobetaine methacrylate) (polySBMA)-grafted surfaces via atom transfer radical polymerization (ATRP) at varying film thicknesses. It is interesting to observe that protein adsorption exhibits a minimum at a medium film thickness. Results show that the surface with 62 nm polySBMA brushes presents the best nonfouling character in 100% Blood Serum and plasma although all of these surfaces are highly resistant to nonspecific protein adsorption from single fibrinogen and lysozyme solutions. Surface resistance to 100% Blood Serum or plasma is necessary for many applications from Blood-contacting devices to drug delivery. This work provides a new in vitro evaluation standard for the application of biomaterials in vivo.

Wei Yang – 2nd expert on this subject based on the ideXlab platform

  • functionalizable and ultra stable nanoparticles coated with zwitterionic poly carboxybetaine in undiluted Blood Serum
    Biomaterials, 2009
    Co-Authors: Wei Yang, Lei Zhang, Shanlin Wang, Andrew D White, Shaoyi Jiang

    Abstract:

    A new surface chemistry presenting an abundance of functional groups for ligand immobilization in an ultra-low fouling background all in one material for nanoparticles was introduced. This surface platform, as demonstrated by zwitterionic poly(carboxybetaine acrylamide) (polyCBAA) coated nanoparticles, is not only ultra stable in undiluted human Blood Serum, but also can be conjugated to biomolecules conveniently and effectively. Thus, this surface chemistry is ideal to create multi-functional nanoparticles for targeted delivery and diagnostics. In addition, this work clearly shows that 10% Blood Serum commonly used to evaluate the stability of nanoparticles is insufficient and a new evaluation criterion with undiluted Blood Serum is recommended.

  • pursuing zero protein adsorption of poly carboxybetaine from undiluted Blood Serum and plasma
    Langmuir, 2009
    Co-Authors: Wei Yang, Wei Li, Jinli Zhang, Shaoyi Jiang

    Abstract:

    Human Blood Serum and plasma pose significant challenges to Blood-contacting devices and implanted materials because of their high nonspecific adsorption onto surfaces. In this work, we investigated nonspecific protein adsorption from single protein solutions and complex media such as undiluted human Blood Serum and plasma onto poly(carboxybetaine acrylamide) (polyCBAA)-grafted surfaces at different temperatures. The polyCBAA grafting was done via atom-transfer radical polymerization (ATRP) with varying film thicknesses. The objective is to create a surface that experiences “zero” protein adsorption from complex undiluted human Blood Serum and plasma. Results show that protein adsorption from undiluted human Blood Serum, plasma, and aged Serum on the polyCBAA-grafted surface is undetectable at both 25 and 37 °C by a surface plasmon resonance (SPR) sensor. This was achieved with a film thickness of ∼21 nm. Furthermore, it is demonstrated that the polyCBAA surfaces after antibody immobilization maintain und…

  • film thickness dependence of protein adsorption from Blood Serum and plasma onto poly sulfobetaine grafted surfaces
    Langmuir, 2008
    Co-Authors: Wei Yang, Wei Li, Jinli Zhang, Shengfu Chen, Gang Cheng, Hana Vaisocherova, Shaoyi Jiang

    Abstract:

    In this work, we investigate protein adsorption from single protein solutions and complex media such as 100% Blood Serum and plasma onto poly(sulfobetaine methacrylate) (polySBMA)-grafted surfaces via atom transfer radical polymerization (ATRP) at varying film thicknesses. It is interesting to observe that protein adsorption exhibits a minimum at a medium film thickness. Results show that the surface with 62 nm polySBMA brushes presents the best nonfouling character in 100% Blood Serum and plasma although all of these surfaces are highly resistant to nonspecific protein adsorption from single fibrinogen and lysozyme solutions. Surface resistance to 100% Blood Serum or plasma is necessary for many applications from Blood-contacting devices to drug delivery. This work provides a new in vitro evaluation standard for the application of biomaterials in vivo.

Stefan M. Waliszewski – 3rd expert on this subject based on the ideXlab platform

  • Organochlorine Pesticide Gradient Levels Among Maternal Adipose Tissue, Maternal Blood Serum and Umbilical Blood Serum
    Bulletin of Environmental Contamination and Toxicology, 2011
    Co-Authors: Margarita Herrero-mercado, Stefan M. Waliszewski, Mario Caba, Carmen Martínez-valenzuela, S. Gomez Arroyo, R. Villalobos Pietrini, P. C. Cantú Martínez, F. Hernández-chalate

    Abstract:

    The objective of the present study was to determine levels and calculate ratios of copartition coefficients among organochlorine pesticides β-HCH, pp′DDE, op′DDT and pp′DDT in maternal adipose tissue, maternal Blood Serum and umbilical Blood Serum of mother-infant pairs from Veracruz, Mexico. Organochlorine pesticides were analyzed in 70 binomials: maternal adipose tissue, maternal Serum and umbilical cord Serum samples, using gas chromatography with electron capture detection (GC-ECD). The results were expressed as mg/kg on fat basis. p,p′-DDE was the major organochlorine component, detected in every maternal adipose tissue (0.770 mg/kg), maternal Serum sample (5.8 mg/kg on fat basis) and umbilical cord Blood sample (6.9 mg/kg on fat basis). p,p′-DDT was detected at 0.101 mg/kg, 2.2 mg/kg and 5.9 mg/kg respectively, according to the order given above. β-HCH was detected at 0.027 mg/kg, 4.2 mg/kg and 28.0 mg/kg respectively. op′DDT was detected only in maternal adipose tissue at 0.011 mg/kg. The copartition coefficients among samples identify significant increases in concentrations from adipose tissue to maternal Blood Serum and to umbilical Blood Serum. The increase indicated that maternal adipose tissue released organochlorine pesticides to Blood Serum and that they are carried over to umbilical cord Blood.

  • Organochlorine Pesticide Levels in Maternal Adipose Tissue, Maternal Blood Serum, Umbilical Blood Serum, and Milk from Inhabitants of Veracruz, Mexico
    Archives of Environmental Contamination and Toxicology, 2001
    Co-Authors: Stefan M. Waliszewski, A. A. Aguirre, Rosa M. Infanzón, C. S. Silva, José Siliceo

    Abstract:

    Organochlorine pesticides, due to their persistence, accumulate in food chains and cause elevated contamination in human beings. These residues bioconcentrate in lipid-rich tissues according to the equilibrium pattern of internal transport and lipid tissue content. The analyses of maternal adipose tissue, maternal Blood Serum, umbilical Blood Serum, colostrum, and mature milk indicate circulation of these compounds through all compartments of the maternal body, including their crossover of the placental barrier. The greatest residue levels found correspond to DDTs, with highest levels determined in colostrum (5.71 mg/kg of DDT total), followed by adipose tissue with 5.66 mg/kg and in mature milk with 4.70 mg/kg. Among DDTs, pp’DDE is the most predominant compound. The paired analyses of organochlorine pesticide residue levels between mother Blood Serum and umbilical Blood Serum demonstrate significant correlation and their transfer from mother to fetus through the placenta. The paired analyses of adipose tissue and colostrum and mature milk contamination levels indicate a high degree of coherence, principally of DDT, in the body and lactation as a decontamination means.