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Mark A Reynolds - One of the best experts on this subject based on the ideXlab platform.
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alveolar ridge and sinus augmentation utilizing platelet rich plasma in combination with freeze dried Bone Allograft case series
Journal of Periodontology, 2000Co-Authors: James D Kassolis, Paul S Rosen, Mark A ReynoldsAbstract:Background: Alveolar Bone regeneration is frequently necessary prior to placement of implants. Efforts to improve wound healing have focused on factors that may enhance Bone formation following guided Bone regeneration (GBR) techniques alone or in combination with Bone replacement graft materials. Recent reports suggest that platelet-rich plasma (PRP), presumably high in levels of peptide growth factors, may enhance the formation of new Bone when used in combination with autogenous graft material. Methods: In this report, the clinical and radiographic results are presented on 15 consecutively treated patients using autologous PRP in combination with freeze-dried Bone Allograft (FDBA) for sinus elevation and/or ridge augmentation. FDBA and PRP (0.5 g/2cc PRP) were mixed and placed as a composite graft material. A gel formed by mixing autologous thrombin-rich plasma with PRP (1:4 ratio) was used to cover the graft material. Core biopsies of grafted areas were obtained in several patients as part of implant ...
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alveolar ridge and sinus augmentation utilizing platelet rich plasma in combination with freeze dried Bone Allograft case series
Journal of Periodontology, 2000Co-Authors: James D Kassolis, Paul S Rosen, Mark A ReynoldsAbstract:BACKGROUND: Alveolar Bone regeneration is frequently necessary prior to placement of implants. Efforts to improve wound healing have focused on factors that may enhance Bone formation following guided Bone regeneration (GBR) techniques alone or in combination with Bone replacement graft materials. Recent reports suggest that platelet-rich plasma (PRP), presumably high in levels of peptide growth factors, may enhance the formation of new Bone when used in combination with autogenous graft material. METHODS: In this report, the clinical and radiographic results are presented on 15 consecutively treated patients using autologous PRP in combination with freeze-dried Bone Allograft (FDBA) for sinus elevation and/or ridge augmentation. FDBA and PRP (0.5 g/2cc PRP) were mixed and placed as a composite graft material. A gel formed by mixing autologous thrombin-rich plasma with PRP (1:4 ratio) was used to cover the graft material. Core biopsies of grafted areas were obtained in several patients as part of implant site preparation and were evaluated histologically to determine site maturation. RESULTS: Of 36 implant fixtures, 32 (89%) were considered clinically successful demonstrating complete Bone coverage of the implant, no mobility, and a normal radiographic appearance at the time of re-entry and 12 months post-implant exposure. Four implants were removed due to mobility at the time of surgical exposure. Histologic evaluation of biopsy specimens revealed numerous areas of osteoid and Bone formation around FDBA particles, with no evidence of inflammatory cell infiltrate. CONCLUSIONS: These clinical and histological findings suggest that ridge augmentation and sinus grafting with FDBA in combination with PRP provide a viable therapeutic alternative for implant placements. Future studies are necessary to determine whether PRP enhances new Bone formation or maturation with Bone replacement Allografts.
Brian L Mealey - One of the best experts on this subject based on the ideXlab platform.
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evaluation of healing following tooth extraction with ridge preservation using cortical versus cancellous freeze dried Bone Allograft
Journal of Periodontology, 2014Co-Authors: Adam J Eskow, Brian L MealeyAbstract:Background: The objective of this study is to compare histologic and clinical healing following tooth extraction and ridge preservation with either cortical or cancellous freeze-dried Bone Allograft (FDBA) in non-molar extraction sockets.Methods: Forty patients requiring implant placement were enrolled, with 20 patients randomly assigned to each group (cortical versus cancellous FDBA). All of the Allograft materials were obtained from the same donor to control for variability between donors and processing. Patients returned after 17 to 21 weeks (average: 18.2 weeks), and a 2-mm-diameter core biopsy was obtained before implant placement. Histomorphometric analysis was performed to determine percentage of new Bone formation, residual graft material, and non-mineralized connective tissue (CT)/other material. Clinical measurements of ridge dimensions were taken at the time of tooth extraction and again at implant placement.Results: There was no significant difference in new Bone formation between the cortical...
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histologic comparison of healing after tooth extraction with ridge preservation using mineralized versus demineralized freeze dried Bone Allograft
Journal of Periodontology, 2012Co-Authors: Robert Wood, Brian L MealeyAbstract:Background: Allografts, such as demineralized freeze-dried Bone Allograft (DFDBA) and mineralized freeze-dried Bone Allograft (FDBA) are commonly used by clinicians for ridge preservation procedures. The primary objective of this study is to histologically evaluate and compare the healing of non-molar extraction sockets grafted with DFDBA versus FDBA for ridge preservation. The secondary aim of this study is to compare dimensional changes in ridge height and width after grafting with these two materials.Materials: Forty patients were randomly divided into two groups of 20. Extraction sockets were filled with either FDBA or DFDBA. To minimize variables associated with the organ donor and with tissue processing, all of the graft material was procured from a single donor; the only difference in the two materials was the percentage mineralization of the final Bone graft. A 2-mm-diameter core biopsy was taken from each grafted site ≈19 weeks after grafting. Histomorphometric analysis was performed to determine...
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histologic analysis of healing after tooth extraction with ridge preservation using mineralized human Bone Allograft
Journal of Periodontology, 2010Co-Authors: Tina M Beck, Brian L MealeyAbstract:Background: Ridge preservation was developed as a therapy to prevent severe Bone resorption after tooth extraction. The purpose of this study is to determine if there is any difference in the amount of new Bone formation 3 months after extraction and ridge preservation compared to that after 6 months. Methods: Minimally traumatic extraction with ridge preservation using mineralized human Bone Allograft was performed at 38 single-rooted tooth sites in 33 subjects. Sixteen sites healed for an average of 14 weeks (early healing), whereas 22 sites were allowed to heal for an average of 27 weeks (delayedhealing)beforeharvestingBonecoresamples.Histomorphometric analysis was performed to determine the percent of new Bone formation, residual graft particles, and connective tissue/non-mineralized structures for each site. Results: All specimens showed evidence of new Bone formation, with most of the residual graft particles surrounded intimately bywovenBone.Nostatisticallysignificant differences in the amount of newly formed Bone or residual graft particles were found between the two groups. Overall, the early healing groupdemonstratedameanof45.8%newBone,14.6%residual graft material, and 39.6% connective tissue/non-mineralized tissue. The delayed healing group showed mean values of 45%, 13.5%, and 41.3%, respectively. Conclusion: The results of this study suggest that waiting 6 months after tooth extraction and ridge preservation using mineralized Bone Allograft does not provide a greater amount ofnew Bone formationorlessresidualBoneparticlescompared to that after only 3 months. J Periodontol 2010;81:1765-1772.
James D Kassolis - One of the best experts on this subject based on the ideXlab platform.
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alveolar ridge and sinus augmentation utilizing platelet rich plasma in combination with freeze dried Bone Allograft case series
Journal of Periodontology, 2000Co-Authors: James D Kassolis, Paul S Rosen, Mark A ReynoldsAbstract:Background: Alveolar Bone regeneration is frequently necessary prior to placement of implants. Efforts to improve wound healing have focused on factors that may enhance Bone formation following guided Bone regeneration (GBR) techniques alone or in combination with Bone replacement graft materials. Recent reports suggest that platelet-rich plasma (PRP), presumably high in levels of peptide growth factors, may enhance the formation of new Bone when used in combination with autogenous graft material. Methods: In this report, the clinical and radiographic results are presented on 15 consecutively treated patients using autologous PRP in combination with freeze-dried Bone Allograft (FDBA) for sinus elevation and/or ridge augmentation. FDBA and PRP (0.5 g/2cc PRP) were mixed and placed as a composite graft material. A gel formed by mixing autologous thrombin-rich plasma with PRP (1:4 ratio) was used to cover the graft material. Core biopsies of grafted areas were obtained in several patients as part of implant ...
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alveolar ridge and sinus augmentation utilizing platelet rich plasma in combination with freeze dried Bone Allograft case series
Journal of Periodontology, 2000Co-Authors: James D Kassolis, Paul S Rosen, Mark A ReynoldsAbstract:BACKGROUND: Alveolar Bone regeneration is frequently necessary prior to placement of implants. Efforts to improve wound healing have focused on factors that may enhance Bone formation following guided Bone regeneration (GBR) techniques alone or in combination with Bone replacement graft materials. Recent reports suggest that platelet-rich plasma (PRP), presumably high in levels of peptide growth factors, may enhance the formation of new Bone when used in combination with autogenous graft material. METHODS: In this report, the clinical and radiographic results are presented on 15 consecutively treated patients using autologous PRP in combination with freeze-dried Bone Allograft (FDBA) for sinus elevation and/or ridge augmentation. FDBA and PRP (0.5 g/2cc PRP) were mixed and placed as a composite graft material. A gel formed by mixing autologous thrombin-rich plasma with PRP (1:4 ratio) was used to cover the graft material. Core biopsies of grafted areas were obtained in several patients as part of implant site preparation and were evaluated histologically to determine site maturation. RESULTS: Of 36 implant fixtures, 32 (89%) were considered clinically successful demonstrating complete Bone coverage of the implant, no mobility, and a normal radiographic appearance at the time of re-entry and 12 months post-implant exposure. Four implants were removed due to mobility at the time of surgical exposure. Histologic evaluation of biopsy specimens revealed numerous areas of osteoid and Bone formation around FDBA particles, with no evidence of inflammatory cell infiltrate. CONCLUSIONS: These clinical and histological findings suggest that ridge augmentation and sinus grafting with FDBA in combination with PRP provide a viable therapeutic alternative for implant placements. Future studies are necessary to determine whether PRP enhances new Bone formation or maturation with Bone replacement Allografts.
Zvi Schwartz - One of the best experts on this subject based on the ideXlab platform.
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clinical evaluation of demineralized Bone Allograft in a hyaluronic acid carrier for sinus lift augmentation in humans a computed tomography and histomorphometric study
Clinical Oral Implants Research, 2007Co-Authors: Zvi Schwartz, Moshe Goldstein, Einav Raviv, A Hirsch, Don M Ranly, Barbara D BoyanAbstract:Objectives: Natural and synthetic graft materials are used routinely in sinus floor augmentations to help support implants in atrophic maxillary ridges. This clinical study was based on the hypothesis that the clinical effectiveness of demineralized freeze-dried Bone Allograft/demineralized Bone matrix (DFDBA/DBM) in sinus lifts varies when used in combination Bone graft substitute materials. To test this hypothesis, DFDBA was used together with one of three materials: in saline plus anorganic Bone (DFDBA: Bio-Oss®); in hyaluronic acid (DFDBA: HY, 32 : 68, w/w; DBX®) alone; DBX® plus Bio-Oss®; and DBX® plus tricalcium phosphate granules (β-TCP). Material and methods: Thirty-two sinus lift procedures, eight per group, were performed on 26 patients. Before surgery and at 8 months post-surgery when implants were placed, ridge heights were visualized by computed tomography (CT) and measured by morphometric analysis. Cores of Bone were removed by trephine at the sites of implant placement; these biopsies from the graft sites were used for histomorphometric analysis. Results: All 32 sinus lift elevations were successful when measured by CT, increasing from an average 2.84±0.2 mm before treatment to 15.2±0.6 mm after treatment. The percent of each biopsy that was occupied by new Bone and incorporated Bone graft materials varied with each treatment: DFDBA+Bio-Oss®, DBX®+Bio-Oss®, or DBX® alone was higher than that for DBX®+β-TCP by approximately 10%. When comparing only newly formed Bone, DBX®+β-TCP treatment resulted in 50% less Bone than the other three preparations. All grafted sites received implants as per the treatment plan for each patient. Conclusions: This study confirmed the hypothesis that new Bone formation is dependent on the DFDBA formulation used and demonstrated that DBX®, alone or in combination with other materials, can be used successfully for sinus floor elevation.
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porcine fetal enamel matrix derivative enhances Bone formation induced by demineralized freeze dried Bone Allograft in vivo
Journal of Periodontology, 2000Co-Authors: Barbara D Boyan, Zvi Schwartz, David L Carnes, David L Cochran, David D Dean, T Weesner, C H Lohmann, D AndreacchioAbstract:Background: Embryonic enamel matrix proteins are involved in the formation of acellular cementum during development of the periodontal attachment apparatus, suggesting that these proteins might be used clinically to promote periodontal regeneration. At present, it is unknown if these proteins are osteoinductive, osteoconductive, or osteopromotive. To address this question, we examined the ability of a commercially prepared embryonic porcine enamel matrix derivative to induce new Bone formation in nude mouse calf muscle, or to enhance the Bone induction ability of a demineralized freeze-dried Bone Allograft (DFDBA). Methods: Porcine fetal enamel matrix derivative (EMD) was implanted bilaterally in the calf muscle of 4 male Nu/Nu mice per treatment group (N = 8 implants): 2 mg EMD alone; 4 mg EMD alone; inactive human DFDBA alone; inactive DFDBA + 2 mg EMD; inactive DFDBA + 4 mg EMD; active DFDBA alone; active DFDBA + 2 mg EMD; and active DFDBA + 4 mg EMD. Implants were harvested after 56 days and examined ...
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ability of commercial demineralized freeze dried Bone Allograft to induce new Bone formation
Journal of Periodontology, 1996Co-Authors: James T. Mellonig, Zvi Schwartz, David L Carnes, J De La Fontaine, David L Cochran, David D Dean, Barbara D BoyanAbstract:Demineralized freeze-dried Bone Allograft (DFDBA) has been used extensively in periodontal therapy. The rationale for use of DFDBA includes the fact that proteins capable of inducing new Bone; i.e., Bone morphogenetic proteins, can be isolated from Bone grafts. Commercial Bone banks have provided DFDBA to the dental practitioner for many years; however, these organizations have not verified the osteoinductive capacity of their DFDBA preparations. The aim of this study was to determine the ability of commercial DFDBA preparations to induce new Bone formation. DFDBA with particle sizes ranging from 200 to 500 μm was received from six Bone banks using various Bone production methods. Different lots of DFDBA from the same tissue bank were sometimes available. A total of 14 lots were examined. The surface area of Bone particles in each sample was measured morphometrically and the pH of a solution containing the particles after suspension in distilled water determined. Samples from each DFDBA lot were implanted...
Judith A Hoyland - One of the best experts on this subject based on the ideXlab platform.
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the use of a novel Bone Allograft wash process to generate a biocompatible mechanically stable and osteoinductive biological scaffold for use in Bone tissue engineering
Journal of Tissue Engineering and Regenerative Medicine, 2015Co-Authors: Christopher A Smith, Stephen M Richardson, M J Eagle, Paul Rooney, Tim N Board, Judith A HoylandAbstract:Fresh-frozen biological Allograft remains the most effective substitute for the 'gold standard' autograft, sharing many of its osteogenic properties but, conversely, lacking viable osteogenic cells. Tissue engineering offers the opportunity to improve the osseointegration of this material through the addition of mesenchymal stem cells (MSCs). However, the presence of dead, immunogenic and potentially harmful Bone marrow could hinder cell adhesion and differentiation, graft augmentation and incorporation, and wash procedures are therefore being utilized to remove the marrow, thereby improving the material's safety. To this end, we assessed the efficiency of a novel wash technique to produce a biocompatible, biological scaffold void of cellular material that was mechanically stable and had osteoinductive potential. The outcomes of our investigations demonstrated the efficient removal of marrow components (~99.6%), resulting in a biocompatible material with conserved biomechanical stability. Additionally, the scaffold was able to induce osteogenic differentiation of MSCs, with increases in osteogenic gene expression observed following extended culture. This study demonstrates the efficiency of the novel wash process and the potential of the resultant biological material to serve as a scaffold in Bone Allograft tissue engineering.