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Michael J Wingfield - One of the best experts on this subject based on the ideXlab platform.
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2004: Lasiodiplodia gonubiensis sp. nov., a new Botryosphaeria anamorph from native Syzygium cordatum
2016Co-Authors: Draginja Pavlic, Michael J Wingfield, Teresa A Coutinho, Marieka Gryzenhout, Botryosphaeria CesAbstract:Abstract: Botryosphaeria spp. are common and widely distributed pathogens on many economically important crops, includ-ing forest tree species. These fungi cause a wide variety of symptoms on trees of all ages, but are mostly associated with canker and die-back of branches and main stems. As disease agents, Botryosphaeria spp. are often encountered in their anamorph state, namely species of Fusicoccum, Diplodia or Lasiodiplodia. During a recent survey of Botryosphaeriaceous fungi from native Syzygium cordatum in South Africa, an unfamiliar Lasiodiplodia sp. was isolated. The aim of this study was to compare this apparently undescribed species with other species of Botryosphaeria using morphological characteristics and DNA sequence data of the rDNA internal transcribed spacers, ITS1 and ITS2. Based on sequence data, the isolates from S. cordatum were more closely related to B. rhodina (anamorph Lasiodiplodia theobromae) than to other Botryosphaeria spp., but also phylogenetically distinct from this species. Conidia of the species from S. cordatum were also different to those of L. theobromae. We conclude that the isolates from S. cordatum represent an undescribed Lasiodiplodia sp. and provide the name Lasiodiplodia gonubiensis for it
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Identification of Botryosphaeriaceae from Eucalyptus, Acacia and Pinus in Venezuela
2016Co-Authors: Sari R. Mohali, Michael J WingfieldAbstract:Species of the Botryosphaeriaceae are pathogens of many plantation trees, including species of Acacia, Eucalyptus and Pinus. Some anamorphs associated with the Botryosphaeriaceae have been reported from Venezuela, but their identification is not certain. The aim of this study was to identify Botryosphaeriaceae affecting plantations of Acacia, Eucalyptus and Pinus in Venezuela. Identifications were made using a combination of morphological characteristics and DNA based molecular techniques, namely comparisons of DNA sequence data and restriction digestion (PCR-RFLP) patterns of ITS rDNA amplicons. From a total of 204 isolates from Venezuela, Botryosphaeria dothidea, B. mamane, the Neofusicoccum ribis / N. parvum complex, N. andinum, Lasiodiplodia theobromae, and Pseudofusicoccum stromaticum were identified. To discriminate between isolates residing in the Neofusicoccum ribis-N. parvum complex, PCR-RFLP patterns for an unidentified DNA region, that were characterised previously, were used. This technique showed that both these species are present in Venezuela. This study represents the first report of Botryosphaeria mamane outside Hawaii and the first records of B. dothidea, Neofusicoccum parvum and N. ribis in Venezuela
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STUDIES IN MYCOLOGY 55: 235–253. 2006. Phylogenetic lineages in the Botryosphaeriaceae
2013Co-Authors: Pedro W. Crous, Michael J Wingfield, Artur Alves, John Rheeder, Walter F. O. Marasas, Alan J. L, Treena Burgess, Paul Barber, Johannes Z GroenewaldAbstract:Abstract: Botryosphaeria is a species-rich genus with a cosmopolitan distribution, commonly associated with dieback and cankers of woody plants. As many as 18 anamorph genera have been associated with Botryosphaeria, most of which have been reduced to synonymy under Diplodia (conidia mostly ovoid, pigmented, thick-walled), or Fusicoccum (conidia mostly fusoid, hyaline, thin-walled). However, there are numerous conidial anamorphs having morphological characteristics intermediate between Diplodia and Fusicoccum, and there are several records of species outside the Botryosphaeriaceae that have anamorphs apparently typical of Botryosphaeria s.str. Recent studies have also linked Botryosphaeria to species with pigmented, septate ascospores, and Dothiorella anamorphs, or Fusicoccum anamorphs with Dichomera synanamorphs. The aim of this study was to employ DNA sequence data of the 28S rDNA to resolve apparent lineages within the Botryosphaeriaceae. From these data, 12 clades are recognised. Two of these lineages clustered outside the Botryosphaeriaceae, namely Diplodia-like anamorphs occurring on maize, which are best accommodated in Stenocarpella (Diaporthales), as well as an unresolved clade including species of Camarosporium/Microdiplodia. We recognise 10 lineages within the Botryosphaeriaceae, including an unresolved clade (Diplodia/Lasiodiplodia/Tiarosporella), Botryosphaeri
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phylogenetic and morphological re evaluation of the Botryosphaeria species causing diseases of mangifera indica
Mycologia, 2005Co-Authors: Bernard Slippers, Teresa A Coutinho, Brenda D Wingfield, Pedro W. Crous, G I Johnson, Michael J WingfieldAbstract:Species of Botryosphaeria are among the most serious pathogens that affect mango trees and fruit. Several species occur on mangoes, and these are identified mainly on the morphology of the an- amorphs. Common taxa include Dothiorella domini- cana, D. mangiferae (5 Natrassia mangiferae), D. aro- matica and an unidentified species, Dothiorella 'long'. The genus name Dothiorella, however, is acknowl- edged as a synonym of Diplodia. This study aimed to characterize and name the Botryosphaeria spp. asso- ciated with disease symptoms on mangoes. To achieve this isolates representing all four Dothiorella spp. mentioned above were compared with the ana- morphs of known Botryosphaeria spp., based on co- nidial morphology and DNA sequence data. Two ge- nomic regions were analyzed, namely the ITS rDNA and b-tubulin regions. The morphological and mo- lecular results confirmed that the fungi previously identified from mango as species of Dothiorella be- long to Fusicoccum. Dothiorella dominicana isolates were identical to isolates of F. parvum (teleomorph 5 B. parva). A new epithet, namely F. mangiferum, is proposed for isolates previously treated as D. man- giferae or N. mangiferae. Isolates of D. aromatica were
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Lasiodiplodia gonubiensis sp. nov., a new Botryosphaeria anamorph from native Syzygium cordatum in South Africa
2004Co-Authors: Draginja Pavlic, Teresa A Coutinho, Bernard Slippers, Marieka Gryzenhout, Michael J WingfieldAbstract:Botryosphaeria spp. are common and widely distributed pathogens on many economically important crops, includ- ing forest tree species. These fungi cause a wide variety of symptoms on trees of all ages, but are mostly associated with canker and die-back of branches and main stems. As disease agents, Botryosphaeria spp. are often encountered in their anamorph state, namely species of Fusicoccum, Diplodia or Lasiodiplodia. During a recent survey of Botryosphaeriaceous fungi from native Syzygium cordatum in South Africa, an unfamiliar Lasiodiplodia sp. was isolated. The aim of this study was to compare this apparently undescribed species with other species of Botryosphaeria using morphological characteristics and DNA sequence data of the rDNA internal transcribed spacers, ITS1 and ITS2. Based on sequence data, the isolates from S. cordatum were more closely related to B. rhodina (anamorph Lasiodiplodia theobromae) than to other Botryosphaeria spp., but also phylogenetically distinct from this species. Conidia of the species from S. cordatum were also different to those of L. theobromae. We conclude that the isolates from S. cordatum represent an undescribed Lasiodiplodia sp. and provide the name Lasiodiplodia gonubiensis for it.
Bernard Slippers - One of the best experts on this subject based on the ideXlab platform.
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seed borne Botryosphaeria spp from native prunus and podocarpus trees in ethiopia with a description of the anamorph diplodia rosulata sp nov
Fungal Biology, 2005Co-Authors: Abdella Gure, Bernard Slippers, Jan StenlidAbstract:Botryosphaeria spp. from seeds of the native afromontane forest tree species, Podocarpus falcatus and Prunus africana in Ethiopia have been identified. This is achieved by combining anamorph morphological characters and ITS sequence data. From a relatively small sample, four Botryosphaeria spp. were encountered. Two of the species from P. falcatus and the one from P. africana have not been previously described. The two species from P. falcatus were represented by only one isolate each and are not named here. The morphology of their Diplodia and Dothiorella anamorphs is, however, characterised. The anamorph of the other Botryosphaeria sp. from P. africana is described here as Diplodia rosulata sp. nov. Furthermore, B. parva is identified from P. falcatus based on ITS phylogeny. This species is also an important pathogen of various commercially important tree species in Ethiopia and elsewhere. This study highlights the ability of Botryosphaeria spp. to infect seeds and the possibility that they might be distributed in this way. The study also contributes to recent attempts to stabilize the taxonomy of Botryosphaeria anamorphs, especially regarding Diplodia which is currently in taxonomic disarray.
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phylogenetic and morphological re evaluation of the Botryosphaeria species causing diseases of mangifera indica
Mycologia, 2005Co-Authors: Bernard Slippers, Teresa A Coutinho, Brenda D Wingfield, Pedro W. Crous, G I Johnson, Michael J WingfieldAbstract:Species of Botryosphaeria are among the most serious pathogens that affect mango trees and fruit. Several species occur on mangoes, and these are identified mainly on the morphology of the an- amorphs. Common taxa include Dothiorella domini- cana, D. mangiferae (5 Natrassia mangiferae), D. aro- matica and an unidentified species, Dothiorella 'long'. The genus name Dothiorella, however, is acknowl- edged as a synonym of Diplodia. This study aimed to characterize and name the Botryosphaeria spp. asso- ciated with disease symptoms on mangoes. To achieve this isolates representing all four Dothiorella spp. mentioned above were compared with the ana- morphs of known Botryosphaeria spp., based on co- nidial morphology and DNA sequence data. Two ge- nomic regions were analyzed, namely the ITS rDNA and b-tubulin regions. The morphological and mo- lecular results confirmed that the fungi previously identified from mango as species of Dothiorella be- long to Fusicoccum. Dothiorella dominicana isolates were identical to isolates of F. parvum (teleomorph 5 B. parva). A new epithet, namely F. mangiferum, is proposed for isolates previously treated as D. man- giferae or N. mangiferae. Isolates of D. aromatica were
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Identification of the causal agent of Botryosphaeria stem canker in Ethiopian Eucalyptus plantations
South African Journal of Botany, 2004Co-Authors: Alemu Gezahgne, Bernard SlippersAbstract:Plantations of exotic Eucalyptus make up more than 30% of Ethiopia's plantations, providing fuel and construction timber to the country. Species such as E. camaldulensis, E. saligna, E. grandis, E. citriodora and E. globulus are most commonly planted. During a survey of Eucalyptus diseases in 2000 and 2001, Botryosphaeria stem canker was observed in most plantations. The disease symptoms included tip die- back, coppice failure and stem cankers characterised by kino exudation. The aim of this study was to identify the species responsible for Botryosphaeria stem canker in Ethiopia. Culture and conidial morphology, as well as DNA-based identification involving Restriction Fragment Length Polymorphisms (RFLPs) and sequencing of the Internal Transcribed Spacer regions (ITS) of the ribosomal RNA gene and the elongation factor 1-alpha (EF1-α) gene, were used to identify isolates. Pathogenicity studies were conducted in the greenhouse and under field conditions. Results showed that Botryosphaeria parva is responsible for Botryosphaeria stem canker of Eucalyptus in Ethiopia. This is the first report of the fungus from this country. Greenhouse and field inoculation studies showed that the Ethiopian isolates are highly virulent. Careful site species selection and breeding trials are thus needed to reduce the impact of this disease in Ethiopia.
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Lasiodiplodia gonubiensis sp. nov., a new Botryosphaeria anamorph from native Syzygium cordatum in South Africa
2004Co-Authors: Draginja Pavlic, Teresa A Coutinho, Bernard Slippers, Marieka Gryzenhout, Michael J WingfieldAbstract:Botryosphaeria spp. are common and widely distributed pathogens on many economically important crops, includ- ing forest tree species. These fungi cause a wide variety of symptoms on trees of all ages, but are mostly associated with canker and die-back of branches and main stems. As disease agents, Botryosphaeria spp. are often encountered in their anamorph state, namely species of Fusicoccum, Diplodia or Lasiodiplodia. During a recent survey of Botryosphaeriaceous fungi from native Syzygium cordatum in South Africa, an unfamiliar Lasiodiplodia sp. was isolated. The aim of this study was to compare this apparently undescribed species with other species of Botryosphaeria using morphological characteristics and DNA sequence data of the rDNA internal transcribed spacers, ITS1 and ITS2. Based on sequence data, the isolates from S. cordatum were more closely related to B. rhodina (anamorph Lasiodiplodia theobromae) than to other Botryosphaeria spp., but also phylogenetically distinct from this species. Conidia of the species from S. cordatum were also different to those of L. theobromae. We conclude that the isolates from S. cordatum represent an undescribed Lasiodiplodia sp. and provide the name Lasiodiplodia gonubiensis for it.
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Development of simple sequence repeat markers for Botryosphaeria spp. with Fusicoccum anamorphs
Molecular Ecology Notes, 2004Co-Authors: Bernard Slippers, Treena I. Burgess, Teresa A Coutinho, Brenda D Wingfield, Michael J WingfieldAbstract:We report the development of eight sets of microsatellite markers for the ascomycete fungus and tree pathogen, Botryosphaeria parva . The primers were identified after cloning and sequencing of fragments amplified using simple sequence repeat (SSR) primers. Genome walking was used to determine unknown sequences on either side of new SSRs. The primers were tested and proved useful in nine other Botryosphaeria species that all have Fusicoccum anamorphs, similar to B. parva .
Teresa A Coutinho - One of the best experts on this subject based on the ideXlab platform.
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2004: Lasiodiplodia gonubiensis sp. nov., a new Botryosphaeria anamorph from native Syzygium cordatum
2016Co-Authors: Draginja Pavlic, Michael J Wingfield, Teresa A Coutinho, Marieka Gryzenhout, Botryosphaeria CesAbstract:Abstract: Botryosphaeria spp. are common and widely distributed pathogens on many economically important crops, includ-ing forest tree species. These fungi cause a wide variety of symptoms on trees of all ages, but are mostly associated with canker and die-back of branches and main stems. As disease agents, Botryosphaeria spp. are often encountered in their anamorph state, namely species of Fusicoccum, Diplodia or Lasiodiplodia. During a recent survey of Botryosphaeriaceous fungi from native Syzygium cordatum in South Africa, an unfamiliar Lasiodiplodia sp. was isolated. The aim of this study was to compare this apparently undescribed species with other species of Botryosphaeria using morphological characteristics and DNA sequence data of the rDNA internal transcribed spacers, ITS1 and ITS2. Based on sequence data, the isolates from S. cordatum were more closely related to B. rhodina (anamorph Lasiodiplodia theobromae) than to other Botryosphaeria spp., but also phylogenetically distinct from this species. Conidia of the species from S. cordatum were also different to those of L. theobromae. We conclude that the isolates from S. cordatum represent an undescribed Lasiodiplodia sp. and provide the name Lasiodiplodia gonubiensis for it
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phylogenetic and morphological re evaluation of the Botryosphaeria species causing diseases of mangifera indica
Mycologia, 2005Co-Authors: Bernard Slippers, Teresa A Coutinho, Brenda D Wingfield, Pedro W. Crous, G I Johnson, Michael J WingfieldAbstract:Species of Botryosphaeria are among the most serious pathogens that affect mango trees and fruit. Several species occur on mangoes, and these are identified mainly on the morphology of the an- amorphs. Common taxa include Dothiorella domini- cana, D. mangiferae (5 Natrassia mangiferae), D. aro- matica and an unidentified species, Dothiorella 'long'. The genus name Dothiorella, however, is acknowl- edged as a synonym of Diplodia. This study aimed to characterize and name the Botryosphaeria spp. asso- ciated with disease symptoms on mangoes. To achieve this isolates representing all four Dothiorella spp. mentioned above were compared with the ana- morphs of known Botryosphaeria spp., based on co- nidial morphology and DNA sequence data. Two ge- nomic regions were analyzed, namely the ITS rDNA and b-tubulin regions. The morphological and mo- lecular results confirmed that the fungi previously identified from mango as species of Dothiorella be- long to Fusicoccum. Dothiorella dominicana isolates were identical to isolates of F. parvum (teleomorph 5 B. parva). A new epithet, namely F. mangiferum, is proposed for isolates previously treated as D. man- giferae or N. mangiferae. Isolates of D. aromatica were
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Lasiodiplodia gonubiensis sp. nov., a new Botryosphaeria anamorph from native Syzygium cordatum in South Africa
2004Co-Authors: Draginja Pavlic, Teresa A Coutinho, Bernard Slippers, Marieka Gryzenhout, Michael J WingfieldAbstract:Botryosphaeria spp. are common and widely distributed pathogens on many economically important crops, includ- ing forest tree species. These fungi cause a wide variety of symptoms on trees of all ages, but are mostly associated with canker and die-back of branches and main stems. As disease agents, Botryosphaeria spp. are often encountered in their anamorph state, namely species of Fusicoccum, Diplodia or Lasiodiplodia. During a recent survey of Botryosphaeriaceous fungi from native Syzygium cordatum in South Africa, an unfamiliar Lasiodiplodia sp. was isolated. The aim of this study was to compare this apparently undescribed species with other species of Botryosphaeria using morphological characteristics and DNA sequence data of the rDNA internal transcribed spacers, ITS1 and ITS2. Based on sequence data, the isolates from S. cordatum were more closely related to B. rhodina (anamorph Lasiodiplodia theobromae) than to other Botryosphaeria spp., but also phylogenetically distinct from this species. Conidia of the species from S. cordatum were also different to those of L. theobromae. We conclude that the isolates from S. cordatum represent an undescribed Lasiodiplodia sp. and provide the name Lasiodiplodia gonubiensis for it.
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Development of simple sequence repeat markers for Botryosphaeria spp. with Fusicoccum anamorphs
Molecular Ecology Notes, 2004Co-Authors: Bernard Slippers, Treena I. Burgess, Teresa A Coutinho, Brenda D Wingfield, Michael J WingfieldAbstract:We report the development of eight sets of microsatellite markers for the ascomycete fungus and tree pathogen, Botryosphaeria parva . The primers were identified after cloning and sequencing of fragments amplified using simple sequence repeat (SSR) primers. Genome walking was used to determine unknown sequences on either side of new SSRs. The primers were tested and proved useful in nine other Botryosphaeria species that all have Fusicoccum anamorphs, similar to B. parva .
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combined multiple gene genealogies and phenotypic characters differentiate several species previously identified as Botryosphaeria dothidea
Mycologia, 2004Co-Authors: Bernard Slippers, Teresa A Coutinho, Brenda D Wingfield, Pedro W. Crous, S Denman, Michael J WingfieldAbstract:Botryosphaeria dothidea is one of the most commonly reported species in a genus of important pathogens of woody plants. This taxon generally is accepted to represent a species complex, and hence its identity remains unclear. Previous studies either have treated B. dothidea as the valid name for B. ribis and B. berengeriana or argued for them to be separate entities. To add to the confusion, no ex-type cultures are available for either B. dothidea or B. ribis. The aim of the present study, therefore, was to recollect and characterize these fungi and designate a set of reference cultures that can be used in future studies. To this end, morphological, cultural and multi-allelic DNA sequence datasets from the rDNA (ITS 1, 5.8S, and ITS 2), b-tubulin and EF1-a genes were used to fully characterize these species. Botryosphaeria dothi- dea was found to be distinct from B. ribis, while B. berengeriana was retained as synonym of the former name. Furthermore, Fusicoccum aesculi is accepted as anamorph of B. dothidea, while the anamorph of B. ribis is newly described as F. ribis sp. nov. Botryosphaer- ia ribis could be distinguished from B. parva based on b-tubulin and EF1-a sequence data. A combined phylogeny of the three gene regions used in this study also showed that the genus Botryosphaeria rep-
Guoping Wang - One of the best experts on this subject based on the ideXlab platform.
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the full length genome sequence of a novel mitovirus from Botryosphaeria dothidea the causal agent of pear ring rot disease
Archives of Virology, 2021Co-Authors: Qi Zou, Ni Hong, Guoping Wang, Yunjing Gao, Qiong Wang, Yuekun Yang, Fang Wang, Liping WangAbstract:Here, we describe a novel mycovirus, tentatively designated as "Botryosphaeria dothidea mitovirus 3" (BdMV3), isolated from Botryosphaeria dothidea strain FJ, which causes pear ring rot disease in Fujian Province, China. The complete genome nucleotide sequence of BdMV3 is 2538 nt in length and contains a single 2070-nt open reading frame (ORF) encoding a putative RNA-dependent RNA polymerase (RdRp) of 689 amino acids (aa) using the fungal mitochondrial genetic code. BLASTp analysis revealed that the RdRp of BdMV3 shares 28.91%–69.36% sequence identity (query sequence coverage more than 90%) with those of members of the genus Mitovirus, with the highest sequence identity of 69.36% and 68.79% to the corresponding RdRp aa sequences of Rhizoctonia solani mitovirus 10 and Macrophomina phaseolina mitovirus 4, respectively. Phylogenetic analysis based on RdRp aa sequences indicated that BdMV3 is a new member of the genus Mitovirus in the family Mitoviridae.
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molecular characterization of a novel mycovirus infecting the phytopathogenic fungus Botryosphaeria dothidea
Archives of Virology, 2020Co-Authors: Mengmeng Yang, Lifeng Zhai, Xiaoqi Zhou, Ni Hong, Feng Xiao, Guoping WangAbstract:In this study, we report the molecular characterization of a novel positive-sense single-stranded RNA (+ssRNA) mycovirus from Botryosphaeria dothidea strain G91, which we name "Botryosphaeria dothidea botourmiavirus 1" (BdBOV-1). The complete genome of BdBOV-1 is 2547 nucleotides (nt) long, contains one open reading frame (ORF) potentially encoding an RNA-dependent RNA polymerase (RdRp), and shows a close relationship to mycoviruses of the family Botourmiaviridae. Phylogenetic analysis based on the RdRp sequences confirmed that BdBOV-1 clustered together with the members of the family Botourmiaviridae. To the best of our knowledge, this is the first report of a +ssRNA mycovirus infecting B. dothidea.
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characterization of a novel victorivirus isolated from the phytopathogenic fungus Botryosphaeria dothidea
Archives of Virology, 2019Co-Authors: Mengmeng Yang, Lifeng Zhai, Ni Hong, Min Fu, Feng Xiao, Guoping WangAbstract:Botryosphaeria dothidea is an important pathogenic fungus that causes serious diseases in fruits and trunks of many wood plant species worldwide. In this study, 28 B. dothidea strains isolated from pear trunk samples showing stem wart or canker symptoms were used to detect double-stranded RNA (dsRNA) viruses. The dsRNA bands with the size of ~ 1.0 to ~ 6.0 kbp were examined from ten strains. Here, we reported a novel dsRNA mycovirus, tentatively named as Botryosphaeria dothidea victorivirus 2 (BdVV2), isolated from the B. dothidea strain MSD53. BdVV2 contained spherical virions that were ~ 38 nm in diameter consisting of a single linear dsRNA genome of 5,090 bp in length. The BdVV2 genome contained two overlapping open reading frames (ORFs) encoding a putative coat protein (CP) and an RNA-dependent RNA polymerase (RdRp), which shared significant amino acid identities of 68% and 60% with the corresponding proteins of Sphaeropsis sapinea RNA viruses 1 (SsRV1). Phylogenetic analyses based on the aa sequences of CP and RdRp both showed that BdVV2 was phylogenetically related to the members of the genus Victorivirus in the family Totiviridae. BdVV2 is thus a novel victorivirus isolated from the phytopathogenic fungus B. dothidea.
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characterization of a novel double stranded rna mycovirus conferring hypovirulence from the phytopathogenic fungus Botryosphaeria dothidea
Virology, 2016Co-Authors: Lifeng Zhai, Zuokun Yang, Ni Hong, Min Fu, Meixin Zhang, Jun Xiang, Guoping WangAbstract:A novel double-stranded RNA (dsRNA) virus, designated as Botryosphaeria dothidea RNA virus 1 (BdRV1), isolated from a hypovirulent strain YZN115 of Botryosphaeria dothidea was biologically and molecularly characterized. The genome of BdRV1 comprises of five dsRNAs. Each dsRNA contains a single open reading frame. The proteins encoded by dsRNA1-4 shared significant amino acid identities of 55%, 47%, 43% and 53% with the corresponding proteins of Aspergillus fumigatus tetramycovirus-1. DsRNA1, 3, and 4 of BdRV1 encoded an RNA-dependent RNA polymerase, a viral methyltransferase, and a P-A-S-rich protein, respectively. Function of proteins encoded by the dsRNA2 and dsRNA5 were unknown. BdRV1 conferred hypovirulence for its host and could be transmitted through conidia and hyphae contact.
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complete dsrna sequence of a novel victorivirus isolated from the pear stem wart fungus Botryosphaeria dothidea
Archives of Virology, 2015Co-Authors: Lifeng Zhai, Ni Hong, Meixin Zhang, Guoping WangAbstract:Botryosphaeria dothidea is a pathogen that causes many important diseases of deciduous fruit trees worldwide. In this study, a double-stranded (ds) RNA of 5322 base pairs (bp) in length from strain GY25 of B. dothidea was isolated and sequenced. The dsRNA potentially contained two overlapping open reading frames (ORFs). The predicted gene product of ORF1 had the highest amino acid sequence similarity, 37 % (E-value, 8e -101 ), to the capsid protein of Rosellinia necatrix victo- rivirus 1 (RnV1), and ORF2 had the highest similarity, 44 % (E-value, 0.0), to the RNA-directed RNA polymerase of Beauveria bassiana victorivirus NZL/1980(BbV-NZL/ 1980). Both of these viruses are members of the genus Victorivirus in the family Totiviridae. There was a small ORF at the 5 0 terminus whose predicted gene product did not show homology to any known proteins. The dsRNA from B. dothidea strain GY25 appears to be a genomic component of a victorivirus, which we have tentatively named ''Botryosphaeria dothidea victorivirus 1'' (BdV1). This is the first full-length genome sequence of a putative victorivirus infecting B. dothidea.
Florence Fontaine - One of the best experts on this subject based on the ideXlab platform.
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cultivar and wood area dependent metabolomic fingerprints of grapevine infected by Botryosphaeria dieback
Phytopathology, 2020Co-Authors: Christelle Lemaitreguillier, Christophe Clément, Florence Fontaine, Chloe Roulliergall, Mourad Harir, Maryline Magninrobert, Sophie Trouvelot, Regis D Gougeon, Philippe Schmittkopplin, Marielle AdrianAbstract:Botryosphaeria dieback is one of the most significant grapevine trunk diseases that affects the sustainability of the vineyards and provokes economic losses. The causal agents, Botryosphaeriaceae species, live in and colonize the wood of the perennial organs causing wood necrosis. Diseased vines show foliar symptoms, chlorosis, or apoplexy, associated to a characteristic brown stripe under the bark. According to the susceptibility of the cultivars, specific proteins such as PR-proteins and other defense-related proteins are accumulated in the brown stripe compared with the healthy woody tissues. In this study, we enhanced the characterization of the brown stripe and the healthy wood by obtaining a metabolite profiling for the three cultivars Chardonnay, Gewurztraminer, and Mourvedre to deeper understand the interaction between the Botryosphaeria dieback pathogens and grapevine. The study confirmed a specific pattern according to the cultivar and revealed significant differences between the brown stripe and the healthy wood, especially for phytochemical and lipid compounds. This is the first time that such chemical discrimination was made and that lipids were so remarkably highlighted in the interaction of Botryosphaeriaceae species and grapevine. Their role in the disease development is discussed.
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Image_1_Bacillus subtilis PTA-271 Counteracts Botryosphaeria Dieback in Grapevine, Triggering Immune Responses and Detoxification of Fungal Phytotoxins.JPEG
2019Co-Authors: Patricia Trotel-aziz, Eliane Abou-mansour, Barbara Courteaux, Fanja Rabenoelina, Christophe Clément, Florence Fontaine, Aziz AzizAbstract:Plant pathogens have evolved various strategies to enter hosts and cause diseases. Particularly Neofusicoccum parvum, a member of Botryosphaeria dieback consortium, can secrete the phytotoxins (-)-terremutin and (R)-mellein during grapevine colonization. The contribution of phytotoxins to Botryosphaeria dieback symptoms still remains unknown. Moreover, there are currently no efficient control strategies of this disease, and agro-environmental concerns have raised increasing interest in biocontrol strategies to limit disease spread in vineyards, especially by using some promising beneficial bacteria. Here, we first examined in planta the biocontrol capacity of Bacillus subtilis PTA-271 against N. parvum Np-Bt67 strain producing both (-)-terremutin and (R)-mellein. We then focused on the direct effects of PTA-271 on pathogen growth and the fate of pure phytotoxins, and explored the capacity of PTA-271 to induce or prime grapevine immunity upon pathogen infection or phytotoxin exposure. Results provided evidence that PTA-271 significantly protects grapevine cuttings against N. parvum and significantly primes the expression of PR2 (encoding a β-1,3-glucanase) and NCED2 (9-cis-epoxycarotenoid dioxygenase involved in abscisic acid biosynthesis) genes upon pathogen challenge. Using in vitro plantlets, we also showed that PTA-271 triggers the expression of salicylic acid- and jasmonic acid-responsive genes, including GST1 (encoding a glutathione-S-transferase) involved in detoxification process. However, in PTA-271-pretreated plantlets, exogenous (-)-terremutin strongly lowered the expression of most of upregulated genes, except GST1. Data also indicated that PTA-271 can detoxify both (-)-terremutin and (R)-mellein and antagonize N. parvum under in vitro conditions. Our findings highlight (-)-terremutin and (R)-mellein as key aggressive molecules produced by N. parvum that may weaken grapevine immunity to promote Botryosphaeria dieback symptoms. However, PTA-271 can efficiently attenuate Botryosphaeria dieback by enhancing some host immune responses and detoxifying both phytotoxins produced by N. parvum.
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Table_1_Bacillus subtilis PTA-271 Counteracts Botryosphaeria Dieback in Grapevine, Triggering Immune Responses and Detoxification of Fungal Phytotoxins.docx
2019Co-Authors: Patricia Trotel-aziz, Eliane Abou-mansour, Barbara Courteaux, Fanja Rabenoelina, Christophe Clément, Florence Fontaine, Aziz AzizAbstract:Plant pathogens have evolved various strategies to enter hosts and cause diseases. Particularly Neofusicoccum parvum, a member of Botryosphaeria dieback consortium, can secrete the phytotoxins (-)-terremutin and (R)-mellein during grapevine colonization. The contribution of phytotoxins to Botryosphaeria dieback symptoms still remains unknown. Moreover, there are currently no efficient control strategies of this disease, and agro-environmental concerns have raised increasing interest in biocontrol strategies to limit disease spread in vineyards, especially by using some promising beneficial bacteria. Here, we first examined in planta the biocontrol capacity of Bacillus subtilis PTA-271 against N. parvum Np-Bt67 strain producing both (-)-terremutin and (R)-mellein. We then focused on the direct effects of PTA-271 on pathogen growth and the fate of pure phytotoxins, and explored the capacity of PTA-271 to induce or prime grapevine immunity upon pathogen infection or phytotoxin exposure. Results provided evidence that PTA-271 significantly protects grapevine cuttings against N. parvum and significantly primes the expression of PR2 (encoding a β-1,3-glucanase) and NCED2 (9-cis-epoxycarotenoid dioxygenase involved in abscisic acid biosynthesis) genes upon pathogen challenge. Using in vitro plantlets, we also showed that PTA-271 triggers the expression of salicylic acid- and jasmonic acid-responsive genes, including GST1 (encoding a glutathione-S-transferase) involved in detoxification process. However, in PTA-271-pretreated plantlets, exogenous (-)-terremutin strongly lowered the expression of most of upregulated genes, except GST1. Data also indicated that PTA-271 can detoxify both (-)-terremutin and (R)-mellein and antagonize N. parvum under in vitro conditions. Our findings highlight (-)-terremutin and (R)-mellein as key aggressive molecules produced by N. parvum that may weaken grapevine immunity to promote Botryosphaeria dieback symptoms. However, PTA-271 can efficiently attenuate Botryosphaeria dieback by enhancing some host immune responses and detoxifying both phytotoxins produced by N. parvum.
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defense responses in grapevine cv mourvedre after inoculation with the Botryosphaeria dieback pathogens neofusicoccum parvum and diplodia seriata and their relationship with flowering
International Journal of Molecular Sciences, 2017Co-Authors: Alessandro Spagnolo, Fanja Rabenoelina, Christophe Clément, Philippe Larignon, Vincenzo Mondello, Sandra Villaume, Florence FontaineAbstract:As a result of the increasing economic impact of grapevine trunk diseases on viticulture worldwide, efficient and viable control strategies are urgently needed. However, understanding both plant-pathogen interactions and plant physiological changes related to these diseases is fundamental to such an achievement. In this study, we analyzed the effect of inoculation with the Botryosphaeria dieback fungal agents, Neofusicoccum parvum and Diplodia seriata, with and without inflorescence removal at the onset of G stage (separated clusters), I stage (flowering) and M stage (veraison). A measure of lesion size and real-time reverse-transcription polymerase chain reaction-based analysis were carried out. The results clearly show the importance of inflorescences in the development of lesions associated with Botryosphaeria dieback pathogens inoculated on green stems of adult vines, especially at the onset of flowering. At flowering, the biggest necroses were observed with the inflorescences present, as well as an activation of the studied defense responses. Thus, an ineffective response to the pathogen could be consistent with a possible metabolic reprogramming linked to the host phenophase.