The Experts below are selected from a list of 75 Experts worldwide ranked by ideXlab platform
Torsten Seuberlich - One of the best experts on this subject based on the ideXlab platform.
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Parainfluenza Virus 5 Infection in Neurological Disease and Encephalitis of Cattle
International Journal of Molecular Sciences, 2020Co-Authors: Melanie M. Hierweger, Simea Werder, Torsten SeuberlichAbstract:The etiology of viral encephalitis in cattle often remains unresolved, posing a potential risk for animal and human health. In metagenomics studies of cattle with Bovine non-suppurative encephalitis, parainfluenza virus 5 (PIV5) was identified in three brain samples. Interestingly, in two of these animals, Bovine herpesvirus 6 and Bovine Astrovirus CH13 were additionally found. We investigated the role of PIV5 in Bovine non-suppurative encephalitis and further characterized the three cases. With traditional sequencing methods, we completed the three PIV5 genomes, which were compared to one another. However, in comparison to already described PIV5 strains, unique features were revealed, like an 81 nucleotide longer open reading frame encoding the small hydrophobic (SH) protein. With in situ techniques, we demonstrated PIV5 antigen and RNA in one animal and found a broad cell tropism of PIV5 in the brain. Comparative quantitative analyses revealed a high viral load of PIV5 in the in situ positive animal and therefore, we propose that PIV5 was probably the cause of the disease. With this study, we clearly show that PIV5 is capable of naturally infecting different brain cell types in cattle in vivo and therefore it is a probable cause of encephalitis and neurological disease in cattle.
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frequency and pathological phenotype of Bovine Astrovirus ch13 neuros1 infection in neurologically diseased cattle towards assessment of causality
Viruses, 2017Co-Authors: Senija Selimovichamza, Anna Oevermann, Céline Louise Boujon, Monika Hilbe, Torsten SeuberlichAbstract:Next-generation sequencing (NGS) has opened up the possibility of detecting new viruses in unresolved diseases. Recently, Astrovirus brain infections have been identified in neurologically diseased humans and animals by NGS, among them Bovine Astrovirus (BoAstV) CH13/NeuroS1, which has been found in brain tissues of cattle with non-suppurative encephalitis. Only a few studies are available on neurotropic Astroviruses and a causal relationship between BoAstV CH13/NeuroS1 infections and neurological disease has been postulated, but remains unproven. Aiming at making a step forward towards assessing the causality, we collected brain samples of 97 cases of cattle diagnosed with unresolved non-suppurative encephalitis, and analyzed them by in situ hybridization and immunohistochemistry, to determine the frequency and neuropathological distribution of the BoAstV CH13/NeuroS1 and its topographical correlation to the pathology. We detected BoAstV CH13/NeuroS1 RNA or proteins in neurons throughout all parts of the central nervous system (CNS) in 34% of all cases, but none were detected in cattle of the control group. In general, brain lesions had a high correlation with the presence of the virus. These findings show that a substantial proportion of cattle with non-suppurative encephalitis are infected with BoAstV CH13/NeuroS1 and further substantiate the causal relationship between neurological disease and Astrovirus infections.
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Bovine Astrovirus infection in feedlot cattle with neurological disease in western Canada.
The Canadian veterinary journal = La revue veterinaire canadienne, 2017Co-Authors: Senija Selimovic-hamza, Sergio Sanchez, Helene Philibert, Edward G. Clark, Torsten SeuberlichAbstract:A novel Bovine Astrovirus (BoAstV CH13/NeuroS1) has been associated with encephalitis in cattle in Europe and the USA. We retrospectively analyzed feedlot cattle with encephalitis of unknown etiology for this virus by in-situ hybridization. Results suggest that BoAstV CH13/NeuroS1 is a major cause of encephalitis in western Canadian feedlot cattle.
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detection of Astrovirus in historical cases of european sporadic Bovine encephalitis switzerland 1958 1976
Frontiers in Veterinary Science, 2016Co-Authors: Senija Selimovichamza, Ilias G. Bouzalas, Anna Oevermann, M Vandevelde, Torsten SeuberlichAbstract:European sporadic Bovine encephalitis is a frequent diagnosis in neurologically diseased cattle, but its etiology remained unresolved. Using in situ hybridization, we have detected a recently discovered neurotropic Bovine Astrovirus in historical tissues in a high proportion of brain samples of affected cattle. Our results suggest that Astroviruses were already involved in the pathogenesis of the disease several decades ago, but have gone undetected.
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Exploring the virome of cattle with non-suppurative encephalitis of unknown etiology by metagenomics
Virology, 2016Co-Authors: Daniel Wüthrich, Céline Louise Boujon, Laura Rosyne Hedwig Truchet, Senija Selimovic-hamza, Anna Oevermann, Ilias G. Bouzalas, Rémy Bruggmann, Torsten SeuberlichAbstract:Non-suppurative encephalitis is one of the most frequent pathological diagnosis in cattle with neurological disease, but there is a gap in the knowledge on disease-associated pathogens. In order to identify viruses that are associated with non-suppurative encephalitis in cattle, we used a viral metagenomics approach on a sample set of 16 neurologically-diseased cows. We detected six virus candidates: parainfluenza virus 5 (PIV-5), Bovine Astrovirus CH13/NeuroS1 (BoAstV-CH13/NeuroS1), Bovine polyomavirus 2 (BPyV-2 SF), ovine herpesvirus 2 (OvHV-2), Bovine herpesvirus 6 (BHV-6) and a novel Bovine betaretrovirus termed BoRV-CH15. In a case-control study using PCR, BoAstV-CH13 (p=0.046), BoPV-2 SF (p=0.005) and BoHV-6 (p=4.3E-05) were statistically associated with the disease. These data expand our knowledge on encephalitis-associated pathogens in cattle and point to the value of NGS in resolving complex infection scenarios in a clinical disease setting.
Mireille Meylan - One of the best experts on this subject based on the ideXlab platform.
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fecal shedding of Bovine Astrovirus ch13 neuros1 in veal calves
Journal of Clinical Microbiology, 2020Co-Authors: Ronja V Kauer, Melanie M. Hierweger, Simea Werder, Michel C Koch, Lutz Schonecker, Jens Becker, Melle Holwerda, Anna N Glaus, Ronald Dijkman, Mireille MeylanAbstract:Specific Astrovirus genotype species are associated with neurological disease and encephalitis in humans, mink, pigs, sheep, and cattle ([1][1]). In humans ([2][2]) and pigs ([3][3]), these encephalitis-associated Astroviruses were also detected in fecal and/or nasopharyngeal samples, suggesting
Melanie M. Hierweger - One of the best experts on this subject based on the ideXlab platform.
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fecal shedding of Bovine Astrovirus ch13 neuros1 in veal calves
Journal of Clinical Microbiology, 2020Co-Authors: Ronja V Kauer, Melanie M. Hierweger, Simea Werder, Michel C Koch, Lutz Schonecker, Jens Becker, Melle Holwerda, Anna N Glaus, Ronald Dijkman, Mireille MeylanAbstract:Specific Astrovirus genotype species are associated with neurological disease and encephalitis in humans, mink, pigs, sheep, and cattle ([1][1]). In humans ([2][2]) and pigs ([3][3]), these encephalitis-associated Astroviruses were also detected in fecal and/or nasopharyngeal samples, suggesting
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Parainfluenza Virus 5 Infection in Neurological Disease and Encephalitis of Cattle
International Journal of Molecular Sciences, 2020Co-Authors: Melanie M. Hierweger, Simea Werder, Torsten SeuberlichAbstract:The etiology of viral encephalitis in cattle often remains unresolved, posing a potential risk for animal and human health. In metagenomics studies of cattle with Bovine non-suppurative encephalitis, parainfluenza virus 5 (PIV5) was identified in three brain samples. Interestingly, in two of these animals, Bovine herpesvirus 6 and Bovine Astrovirus CH13 were additionally found. We investigated the role of PIV5 in Bovine non-suppurative encephalitis and further characterized the three cases. With traditional sequencing methods, we completed the three PIV5 genomes, which were compared to one another. However, in comparison to already described PIV5 strains, unique features were revealed, like an 81 nucleotide longer open reading frame encoding the small hydrophobic (SH) protein. With in situ techniques, we demonstrated PIV5 antigen and RNA in one animal and found a broad cell tropism of PIV5 in the brain. Comparative quantitative analyses revealed a high viral load of PIV5 in the in situ positive animal and therefore, we propose that PIV5 was probably the cause of the disease. With this study, we clearly show that PIV5 is capable of naturally infecting different brain cell types in cattle in vivo and therefore it is a probable cause of encephalitis and neurological disease in cattle.
Ricardo Luiz Moro Sousa - One of the best experts on this subject based on the ideXlab platform.
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Molecular detection and phylogenetic analysis of Bovine Astrovirus in Brazil
Archives of Virology, 2015Co-Authors: Marcelo Candido, Anna Luiza Farias Alencar, Sabrina R. Almeida-queiroz, Maria Da Glória Buzinaro, Flavia Simone Munin, Silvia Helena Seraphin Godoy, Marcia Cristina Livonesi, Andrezza Maria Fernandes, Ricardo Luiz Moro SousaAbstract:Bovine Astrovirus (BoAstV) is associated with gastroenterical disorders such as diarrhea, particularly in neonates and immunocompromised animals. Its prevalence is >60 % in the first five weeks of the animal’s life. The aim of this study was to detect and perform a phylogenetic analysis of BoAstV in Brazilian cattle. A prevalence of 14.3 % of BoAstV in fecal samples from 272 head of cattle from different Brazilian states was detected, and 11 samples were analyzed by nucleotide sequencing. The majority of positive samples were obtained from diarrheic animals ( p 74.8 % when compared with reference samples for enteric BoAstV. Our results indicate, for the first time, the occurrence of BoAstV circulation in cattle from different regions of Brazil, prevalently in diarrheic calves.
Makoto Nagai - One of the best experts on this subject based on the ideXlab platform.
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Whole genome analysis of a novel neurotropic Bovine Astrovirus detected in a Japanese black steer with non-suppurative encephalomyelitis in Japan
Archives of Virology, 2018Co-Authors: Yoshimasa Hirashima, Tsutomu Omatsu, Daisuke Okada, Shoichi Shibata, Shu Yoshida, Shoichiro Fujisono, Tetsuya Mizutani, Makoto NagaiAbstract:While neurotropic Bovine Astroviruses (BoAstVs) have been identified in North America and Europe, their presence has never been reported in Asia. In this study, we detected BoAstV in the brain of a steer showing neurological signs. Phylogenetic analysis revealed that the identified virus belongs to the Virginia/Human-Mink-Ovine clade, which contains most of the neurotropic Astroviruses including the neurotropic BoAstVs. Similarity plot analysis showed that the virus was closely related to the American BoAstV NeuroS1 strain with respect to the ORF regions and to the European BoAstV CH13 strain in the 3′ untranslated region, suggesting the occurrence of intra-genotypic recombination events.
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Full genome analysis of Bovine Astrovirus from fecal samples of cattle in Japan: identification of possible interspecies transmission of Bovine Astrovirus
Archives of Virology, 2015Co-Authors: Makoto Nagai, Tsutomu Omatsu, Hiroshi Aoki, Konosuke Otomaru, Takehiko Uto, Motoya Koizumi, Fujiko Minami-fukuda, Hikaru Takai, Toshiaki Murakami, Tsuneyuki MasudaAbstract:A viral metagenomics approach was used to investigate fecal samples of Japanese calves with and without diarrhea. Of the different viral pathogens detected, read counts gave nearly complete Astrovirus-related RNA sequences in 15 of the 146 fecal samples collected in three distinct areas (Hokkaido, Ishikawa, and Kagoshima Prefectures) between 2009 and 2015. Due to the lack of genetic information about Bovine Astroviruses (BoAstVs) in Japan, these sequences were analyzed in this study. Nine of the 15 Japanese BoAstVs were closely related to Chinese BoAstVs and clustered into a lineage (tentatively named lineage 1) in all phylogenetic trees. Three of 15 strains were phylogenetically separate from lineage 1, showing low sequence identities, and clustered instead with an American strain isolated from cattle with respiratory disease (tentatively named lineage 2). Interestingly, two of 15 strains clustered with lineage 1 in the open reading frame (ORF)1a and ORF1b regions, while they clustered with lineage 2 in the ORF2 region. Remarkably, one of 15 strains exhibited low amino acid sequence similarity to other BoAstVs and was clustered separately with porcine Astrovirus type 5 in all trees, and ovine Astrovirus in the ORF2 region, suggesting past interspecies transmission.