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Isobel A P Parkin – 1st expert on this subject based on the ideXlab platform
the pangenome of an agronomically important crop plant Brassica OleraceaNature Communications, 2016Co-Authors: Agnieszka A Golicz, Isobel A P Parkin, Philipp E Bayer, Guy C Barker, Patrick P Edger, Paula Martinez, Chonkit Kenneth Chan, Anita Severnellis, Richard W Mccombie, Andrew H PatersonAbstract:
There is an increasing awareness that as a result of structural variation, a reference sequence representing a genome of a single individual is unable to capture all of the gene repertoire found in the species. A large number of genes affected by presence/absence and copy number variation suggest that it may contribute to phenotypic and agronomic trait diversity. Here we show by analysis of the Brassica Oleracea pangenome that nearly 20% of genes are affected by presence/absence variation. Several genes displaying presence/absence variation are annotated with functions related to major agronomic traits, including disease resistance, flowering time, glucosinolate metabolism and vitamin biosynthesis.
the Brassica Oleracea genome reveals the asymmetrical evolution of polyploid genomesNature Communications, 2014Co-Authors: Xinhua Yang, Chaobo Tong, David Edwards, Isobel A P Parkin, Meixia Zhao, Jingyin Yu, Shunmou Huang, Xiyin Wang, Junyi WangAbstract:
Brassica Oleracea is plant species comprising economically important vegetable crops. Here, the authors report the draft genome sequence of B. Oleracea and, through a comparative analysis with the closely related B. rapa, reveal insights into Brassica evolution and divergence of interspecific genomes and intraspecific subgenomes.
alignment of the conserved c genomes of Brassica Oleracea and Brassica napusTheoretical and Applied Genetics, 1996Co-Authors: E. J. R. Bohuon, Isobel A P Parkin, D J Keith, Andrew G Sharpe, D. J. LydiateAbstract:
A population of 169 microspore-derived doubled-haploid lines was produced from a highly polymorphic Brassica Oleracea cross. A dense genetic linkage map of B. Oleracea was then developed based on the segregation of 303 RFLP-defined loci. It is hoped that these lines will be used by other geneticists to facilitate the construction of a unified genetic map of B. Oleracea. When the B. Oleracea map was compared to one ofB. napus (Parkin et al. 1995), based on the same RFLP probes (Sharpe et al. 1995), good collinearity between the C-genome linkage groups of the two species was observed.
Honghao Lv – 2nd expert on this subject based on the ideXlab platform
chromosome doubling of microspore derived plants from cabbage Brassica Oleracea var capitata l and broccoli Brassica Oleracea var italica lFrontiers in Plant Science, 2015Co-Authors: Suxia Yuan, Yanbin Su, Zhansheng Li, Zhiyuan Fang, Limei Yang, Mu Zhuang, Yangyong Zhang, Honghao LvAbstract:
Chromosome doubling of microspore-derived plants is an important factor in the practical application of microspore culture technology because breeding programs require a large number of genetically stable, homozygous doubled haploid plants with a high level of fertility. In the present paper, 29 populations of microspore-derived plantlets from cabbage (Brassica Oleracea var. capitata) and broccoli (Brassica Oleracea var. italica) were used to study the ploidy level and spontaneous chromosome doubling of these populations, the artificial chromosome doubling induced by colchicine, and the influence of tissue culture duration on the chromosomal ploidy of the microspore-derived regenerants. Spontaneous chromosome doubling occurred randomly and was genotype dependent. In the plant populations derived from microspores, there were haploids, diploids, and even a low frequency of polyploids and mixed-ploidy plantlets. The total spontaneous doubling in the 14 cabbage populations ranged from 0 – 76.9%, compared with 52.2 – 100% in the 15 broccoli populations. To improve the rate of chromosome doubling, an efficient and reliable artificial chromosome doubling protocol (i.e., the immersion of haploid plantlet roots in a colchicine solution) was developed for cabbage and broccoli microspore-derived haploids. The optimal chromosome doubling of the haploids was obtained with a solution of 0.2% colchicine for 9 – 12 h or 0.4% colchicine for 3 – 9 h for cabbage and 0.05% colchicine for 6 – 12 h for broccoli. This protocol produced chromosome doubling in over 50% of the haploid genotypes for most of the populations derived from cabbage and broccoli. Notably, after 1 or more years in tissue culture, the chromosomes of the haploids were doubled, and most of the haploids turned into doubled haploid or mixed-ploidy plants. This is the first report indicating that tissue culture duration can change the chromosomal ploidy of microspore-derived regenerants.
Ramar Thangam – 3rd expert on this subject based on the ideXlab platform
antioxidant and in vitro anticancer effect of 2 pyrrolidinone rich fraction of Brassica Oleracea var capitata through induction of apoptosis in human cancer cellsPhytotherapy Research, 2013Co-Authors: Ramar Thangam, Veeraperumal Suresh, Mayan Rajkumar, Jally Damien Vincent, Palani Gunasekaran, Chinnathambi Anbazhagan, Krishnasamy Kaveri, S KannanAbstract:
The aim of this study was to analyze if the 2-pyrrolidinone rich fraction of Brassica Oleracea var. capitata exhibiting antioxidant and in vitro anticancer activities. 2-Pyrrolidinone is an active compound present in Brassica Oleracea var. capitata. Our findings explored the potential use of 2-pyrrolidinone in cancer treatment. This compound was identified and isolated by gas chromatography–mass spectrometry and high-performance liquid chromatography from the leaf of Brassica Oleracea var. capitata. The resultant rich active compound exhibited in vitro cytotoxicity in HeLa and PC-3 human cancer cell lines, and it also exhibited antioxidant activity in cell free assays. DAPI staining, an apoptotic analysis and cell cycle analysis were performed to evaluate the anticancer activity of 2-pyrrolidinone against the above cell lines. The IC50 value of 2-pyrrolidinone was determined to be of 2.5 µg/ml for HeLa, 3 µg/ml for PC-3 cells at 24 h and 1.5 µg/ml for HeLa and 2 µg/ml for PC-3 cells at 48 h, respectively. However, cell cycle analysis revealed that the anti-proliferative effects of the 2-pyrrolidinone were mediated through cell cycle arrest in the G0/G1 phase. These results from the current study suggest that the 2-pyrrolidinone have potential anticancer effects, which will lead to the development of new anticancer agents for arresting cancer cells growth in vitro. Copyright © 2012 John Wiley & Sons, Ltd.